49
April 2013 Volume 35 NO. 1 ISSN 0276-4776 Spring nEWSLETTER Oklahoma Microscopy Society
April 2013 Volume 35 NO. 1 ISSN 0276-4776
Spring nEWSLETTER Oklahoma Microscopy Society
i
About the cover . . .
Female Tabanus lineola Horse Fly photographed by Thomas Shahan
Thomas Shahan has an exhibit of macro photographs he makes of insects and spi-
ders on display at the Sam Noble Oklahoma Museum of Natural History as part of a large scale exhibit of Oklahoma arthropods. The display is to be shown through September 8th 2013.
Thomas who now works as an imaging special-
ist at the Oregon Department of Agriculture’s entomology lab first began photographing ―bugs‖ when he was a teenager attending Jenks High School in Oklahoma.
I'm not sure if a fly can be any more beautiful
than a female Striped Horse Fly (Tabanus lineo-la) and I doubt I could have found a more perfect specimen. I've been planning this shot for years now, and as I left the house heading out to my favorite bug spot the day that I took this photo, I thought to myself "how great would it be if I could find a cooperative T. lineola female to-day?"
And sure enough - almost as soon as I arrived
at the park, I spotted a large female cleaning her eyes on the railing of the bridge I find salticids on! She was quite battleworn and uncooperative, and I gave up chas-ing her around pretty quickly. Soon after, another female landed nearby (the fly pic-tured above and below), and instantly caught my eye - she was smaller and cleaner with brighter, more deeply saturated eyes. I proceeded to photograph her from just about every imaginable angle and magnification I am capable of, and she sat coop-eratively the entire time despite grooming her eyes and wandering about a bit.
Oklahoma Microscopy Society members who are attending the spring meet-
ing (April 12, 2013) will get free entry into the Sam Noble Museum of Natural History to view Thomas Shahans’ exhibit. Just stop by the main desk and give your name to the attendant for entry into the museum. OU, OU-HSC, OU-Tulsa students, faculty and staff will also have free entry by showing their ID cards.
For more information about Thomas Shahan see …
Thomas Shahan; Arthropod photographer. By Bradley Morris, Tulsa People, March 2013.
http://www.thomasshahan.com Images used under Creative Commons license—http://creativecommons.org/licenses/by-nc-nd/2.0/ Image rights reserved Thomas Shahan
President’s Letter ................................................................................... 1
OMS Officers for 2012-2013..................................................................... 2
Corporate Members ................................................................................3-4
Professional Members ............................................................................4-7
Student Members ....................................................................................7-8
Upcoming Microscopy Meetings .............................................................. 9
OMS Member News .......................................................................... 10-11
Ugly Bug Presentations 2012 .............................................................12-13
Spring Workshop 2013 .......................................................................14-23
Announcement .................................................................................... 14
Kids Night with a Scanning Electron Microscope ............................. 15
Program ............................................................................................... 16
Registration ......................................................................................... 17
Directions & Map—Meeting Site .................................................18-20
Abstracts ........................................................................................21-24
Student Micrograph Contest ............................................................... 25
OMS Constitution and Bylaws ...........................................................26-29
OMS Membership Application/Renewal Form ....................................... 30
List of Advertisers .................................................................................... 31
Advertisements ...................................................................................32-44
Table of Contents
Greg Strout, Editor
Samuel Roberts Noble Microscopy
Laboratory
770 Van Vleet Oval
Norman, OK 73071
ii
1
President’s letter
Dear OMS members, As a relatively fresh face in the OMS, I can’t comment overarchingly about the history and trends in OMS membership and activities. Instead, I can look with humility at the efforts of many members and that have cultivated and grown successful OMS activi-ties, while also dedicating their time to the nuts-and-bolts care and maintenance of the OMS. We should all send a big THANK YOU to all those unsung heroes of member-ships, check-cashing and writing, mailing, web updates, newsletter writing
The UglyBug project stands out as an example of exceptional commitment. The ’core’ UglyBug team really makes the magic happen. Working with sponsors such as Phillips 66, Leica, and Terminix, stuffing envelopes for mailings and sending communications, imaging dozens of bugs, designing posters, and making presentation trips to schools describe just some of the actions selflessly completed by the team. We should send another big THANK YOU to the UglyBug team for an amazing example of science and technology outreach that truly spreads across Oklahoma.
Over the last fall, updates were completed on the Ugly Bug website (uglybug.org). Take a look and send your inspired suggestions!
Another aspect of the OMS that impresses a relative newcomer is the level of support. Financial support from sponsors enables the OMS to follow through on the creative programs, endeavors, and ideas brought by its members. Sponsorship also includes personal commitment demonstrated through vendor exhibitions and participation in events such as Kids Nights.
Coming now to events that I can provide information about: the spring meeting is just ahead on our doorstep. The program highlights advances in 3D-spatial interrogation using microscopes that transcend the collection of images. In particular, the ability of scanning probe microscopes to manipulate materials and measure a variety of proper-ties will be discussed in a series of cross-disciplinary talks. Then, we will have the priv-ilege of learning about advances in confocal imaging and analysis that can be applied using technology now available at OU.
Take a moment to save the date on your calendars: the OMS fall technical meeting / Oklahoma Academy of Science Fall meeting will be at Cameron University on Novem-ber 8th, 2013.
Before signing off, I would like to single out Scott Russell with a giant ―thank you‖ for his time, energy, and experience in wisely and patiently fielding the barrage of ques-tions I have flung at him.
I look forward to seeing you all at the meetings, or elsewhere around Oklahoma! Yours sincerely,
Andy S. Elwood Madden
Officers 2012-2013
President: Elison Blancaflor Samuel Roberts Noble Foundation Plant Biology Division 2510 Sam Noble Parkway Ardmore, OK 73401 (580) 224-6687 [email protected] Past President: Scott Russell Dept. Botany and Microbiology University of Oklahoma 770 Van Vleet Oval Norman, OK 73019 (405) 325-4391 [email protected] Newsletter Editor: Greg Strout University of Oklahoma 770 Van Vleet Oval Norman, OK 73019 (405) 325-4391 [email protected] Corporate Rep: Kevin Cronyn Hitachi High Technologies America PO Box 612208 Irving, TX 75261 (972) 615-9086 [email protected] Physical Sci. Rep: Mark Curtis University of Oklahoma Petroleum & Geological Engineering Sasrkeys Energy Center (405) 325-1719 [email protected]
President-Elect: Andrew Madden Dept. of Geology and Geophysics University of Oklahoma Sarkeys Energy Center, Suite 710 Norman, OK 73019 (405) 325-5327 [email protected] Secretary-Treasurer: Bill Meek Dept. of Anatomy and Cell Biology OSU-Center for Health Sciences 1111 W. 17
th Street
Tulsa, OK 74107 (918) 561-8258 [email protected] Student Representative: Danny Maples Oklahoma State University Department of Chemistry 13 Summit Circle Stillwater, OK 74045 (405)334-6902 [email protected] Biological Sci. Rep: Lisa Whitworth OSU Microscopy Laboratory Venture 1 1110 S. Innovation Way Stillwater, OK 74074 Phone: (405) 744-6765 [email protected]
2
President: Andrew Madden Dept. of Geology and Geophysics University of Oklahoma Sarkeys Energy Center, Suite 710 Norman, OK 73019 (405) 325-5327 [email protected] Past-President: Elison Blancaflor Samuel Roberts Noble Foundation Plant Biology Division 2510 Sam Noble Parkway Ardmore, OK 73401 (580) 224-6687 [email protected] Newsletter Editor: Greg Strout University of Oklahoma 770 Van Vleet Oval Norman, OK 73019 (405) 325-4391 [email protected] Corporate Rep: Kevin Cronyn Hitachi High Technologies America PO Box 612208 Irving, TX 75261 (972) 615-9086 [email protected] Physical Sci. Rep: Dachuan Shi CBME University of Oklahoma 100 E Boyd Street Norman, OK 73019 (405) 708-8940 [email protected]
President-Elect: Jin Nakashima Core Cellular Imaging, Plant Biology Div. Samuel Roberts Noble Foundation Plant Biology Division 2510 Sam Noble Parkway Ardmore, OK 73401 (580) 224-6756 [email protected] Secretary-Treasurer: Bill Meek Dept. of Anatomy and Cell Biology OSU-Center for Health Sciences 1111 W. 17
th Street
Tulsa, OK 74107 (918) 561-8258 [email protected] Student Representative: Ernie Sanchez 1412 Garfield Norman, OK 73019 (405)812-0448 [email protected] Biological Sci. Rep: Lisa Whitworth OSU Microscopy Laboratory Venture 1 1110 S. Innovation Way Stillwater, OK 74074 Phone: (405) 744-6765 [email protected]
3
Corporate Members 2012-2013
Angelique Graves
Sales Executive
Leica Microsystems, Inc.
1700 Leider Lane
Buffalo Grove, IL
(713)823-5366
Angelique.graves@leica-
microsystems.com
John Haritos
Oxford Instruments America,
Inc.
300 Baker Avenue Suite 150
Concord, MA 01742
(978) 369-9933
Alan Hollaar
Senior Sales Engineer
Bruker Nano Inc.
12565 Spring Creek Road
Moorpark, CA 93021
(805) 523-1882
FAX: (805) 426-8052
Stacie Kirsch, EMS/Diatome
P.O. Box 550
1560 Industry Road
Hatfield, PA 19440
(215) 412-8400
David Leland
Thermo Electron Corp.
5225 Verona Road
Madison, WI 53771-4495
(970) 266-1166
James Long
Sales Manager
IXRF Systems, Inc.
3019 Alvin DeVane Blvd.
Suite 130
Austin, TX 789741
(512)386-6100
Zane Marek
JEOL U.S.A. Inc.
13610 Paisano Circle
Austin, TX 78737
(978) 495-2176
Mark T. Nelson
Microscopy Innovations
213 Air Park Rd, Suite 101
Marshfield, WI 54449
(715)384-3292
Mark.nelson@microscopyinnova
tions.com
Janice G. Pennington
Microscopy Innovations
5200 Sassafras Drive
Fitchburg, WI 53711
(317)420-3676
Mark Richardson
Carl Zeiss MicroImaging, Inc.
Thornwood, NY 10594
800-543-1033 VM Box #7275
Eugene Rodek
SPI Supplies
569 E. Gay Street
West Chester, PA 19381
(610) 436-5400 X 109
Cathy Ryan
Micro Star Technologies Inc.
511 FM 3179
Huntsville, TX 77340-2069
(936) 291-6891
800-533-2509
Chad M. Tabatt
Gatan, INC
5933 Coronodo Ln.
Pleasanton, CA 94588
(925) 224-7318
Matt Chipman
EDAX INC.
91 McKee Drive
Mahwah, NJ 07430
Fax: (201) 529-3156
(201) 529-6277
Kevin M. Cronyn
Hitachi High Technologies
American
1401 North 27th Ave.
P.O. Box 612208
Dallas (DFW Airport), TX
75261-2208
(972) 615-9086
Kevin.Cronyn@hitachi-
HHTA.com
Melissa Dubitsky
Tousimis Research Corporation
2211 Lewis Avenue
Rockville, MD 20851
(301) 881-2450
Christine Frey
Hitschfel Instruments, Inc.
2333 S Hanley Road
St. Louis, Mo 63144
(314) 644-6660
Leon Gawlick
McBain Sys./McBain Inst.
6565 MacArthur Blvd. Ste. 225
Irving, TX 75039
(214) 952-5946
Steven Goodman
Microscopy Innovations
13 Mark Twain Street
Madison, WI 53705
(608)236-0627
Ste-
ven.goodman@microscopyinnov
ations.com
4
Professional Members 2012-2013
Corporate Members 2012-2013
Tina Wolodkowicz
EDAX,/AMETEK
91 McKee Dr.
Mahwah, NJ 07430
(201) 529-6277 [email protected]
Jack Vermeulen
Ted Pella Inc.
P.O. 492477
Redding, CA 96049-2477
1-800-237-3526 Ext. 205
FAX: 530-243-3761
Lloyd Walker
Nikon Instruments Oklahoma
Okla.Bioscience/Industrial In-
str.
1955 Lakeway Dr., Suite 250B
Lewisville, TX 75057
888-424-0880
Kenny Witherspoon
IXRF Systems, Inc.
15715 Brookford Dr.
Houston TX 77059
281-286-6485
Kenneth Andrews
Department of Biology
East Central University
Ada, OK 74820
(580) 310-5496
Laura Bartley
Dept. Botany & Microbiology
770 Van Vleet Oval
University of Oklahoma
Norman, OK 73019-0245
(405) 325-1653
Elison B. Blancaflor
Samuel Roberts Noble Fnd.
Plant Biology Division
2510 Sam Noble Parkway
Ardmore, OK 73401
(580) 224-6687
Ying Chen
OUHSC
941 Stanton Young Blvd.
Oklahoma City, OK 73104
(405) 271-4629
William F. Chissoe
1849 Creekside Drive
Norman, OK 73071
(405) 329-0271
Lifetime member
Terry Colberg
OSU Microscopy Laboratory
Venture 1
1110 S. Innovation Way
Stillwater, OK 74074
Phone: (405) 744-6765
Mark E. Curtis
University of Oklahoma
Petroleum & Geological Engin.
Sasrkeys Energy Center
(405) 325-1719
XinShun Ding
Plant Biology Division
The Noble Foundation
2510 Sam Noble Parkway
P.O. Box 2180
Ardmore, OK 73401
(580) 224-6622
5
Phoebe J. Doss
EM, Alcon Research, LTD.
6201 South Freeway
Fort Worth, TX 76134-2099
(817) 568-6090
Terry Dunn
College of Medicine
Dept. of Pathology
OU Health Sciences Center
Oklahoma City, OK 73190
(405) 271-5249
Chris Edwards
Halliburton Energy Services
2600 S 2nd Street #0470
Duncan, OK 75536
(580) 251-3270
FAX: (405) 251-4745
Steve Fields
Department of Biology
East Central University
1100 E. 14th Street
Ada, OK 74820
(580) 559-5792/5606
Warren Finn
Dept. of Pharm/Physiology
OSU-Center for Health Sciences
1111 West 17th Street
Tulsa, OK 74107-1898
(918) 561-8276
Taylor Fore
University of Oklahoma
Department of Zoology
730 Van Vleet Oval
Norman, OK 73019
(405) 325-7450
Ben Fowler, OMRF
825 NE 13th Street, MS 49
Oklahoma City, OK 73106
(405)271-7245
Ginger Hendricks
8804 E. 63rd Street
Tulsa, OK 74133
(918) 294-3992
Kirby L. Jarolim
OSU-CHS
Oklahoma State University
1111 W. 17th Street
Tulsa, OK 74107
(918) 561-8265
Matthew B. Johnson
Dept. Physics & Astronomy
University of Oklahoma
440 West Brooks
Norman, OK 73019-0225
(405) 325-3961 ext. 36106 [email protected]
Paige Johnson
Dept. Chemistry & Biochemistry
University of Tulsa
600 S. College
Tulsa, OK 74104
(918) 631-5434
Naji Khoury
CEED, University of Oklahoma
202 West Boyd Street, Room 334
Norman, OK 73019
(405) 325-4236
Katherine M. Kocan
Dept. Vet. Pathobiology
OSU-Stillwater
250 McElroy Hall CVM
Stillwater, OK 74078
(405) 744-7271
Preston Larson
University of Oklahoma
Samuel Roberts Noble Electron
Microscopy Laboratory
770Van Vleet Oval
Norman, OK 73019
(405) 325-4391
Joanna Ledford
Biochemistry & Mol. Biology
246 NRC
OSU-Stillwater
Stillwater, OK 74078
(405) 744-7822
Tiffany Lenhart
Dept. Botany & Microbiology
770 Van Vleet Oval
University of Oklahoma
Norman, OK 73019-0245
(405) 325-3771
David London
School of Geology &
Geophysics
100 E. Boyd St., 810 SEC
University of Oklahoma
Norman, OK 73019
(405) 325-7626
Gary Lovell
ConocoPhillips Petroleum
245a GB
Bartlesville, OK 74004
(918) 661-9691
Jeanmarie Verchot Lubicz
OSU-Entomology/Plant Patholo-
gy
Noble Research Center, Rm. 127
Stillwater, OK 74078
(405) 744-7895
Professional Members 2012-2013
6
Professional Members 2012-2013
Andrew Madden
Dept. of Geology and Geophysics
University of Oklahoma
Sarkeys Energy Center, Suite 710
Norman, OK 73019
(405) 325-5327
Camelia Maier
Dept. of Biology, GRB 328
Texas Women’s University
Denton, TX 76204
(940) 898-2358
Leanne Wier May
Rose State College
6420 SE 15th Street
Engineering & Science Division
Midwest City, OK 73110
(405) 733-7553
Donna McCall
Halliburton Energy Services
2600 South 2nd Street
Duncan, OK 73533
(580) 251-2083
Jeff McCosh
Dept. Anatomy & Cell Biology
OSU-Center for Health Sciences
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8242
Bill Meek
Dept. of Anat. & Cell Biology
OSU-Center for Health Sciences
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8258
Wilson Merchan-Merchan
School of Aerospace & Mech Eng
University of Oklahoma
865 Asp Avenue, Room 208
Norman, OK 73019-1052
(405) 325-1754
George B. Morgan VI
Electron Microprobe Lab
School Geology & GeoPhysics
100 E. Boyd St., SEC 810
University of Oklahoma
Norman, OK 73019-1009
Jin Nakashima
The Samuel Roberts Noble Foun-
dation
2510 Sam Noble Parkway
Ardmore, OK 73401
(580)224-6756
Richard S. Nelson
Samuel Roberts Noble Founda-
tion
P.O. Box 2180
Ardmore, OK 73402
(580) 224-6625
Charlotte L. Ownby
OSU Microscopy Laboratory
1110 S. Innovation Way
Stillwater, OK 74074
(405) 744-8087
Kevin Pargeter
PO Box 177
Jenks, OK 74037
Dean Phillips
Conoco Phillips
312 South Chickasaw
Bartlesville, OK 74003
(918) 661-8733
Richard W. Portman
University of Tulsa
Dept. of Biological Sciences
600 S. College
Tulsa, OK 74104
(918) 631-3715
James Posey
Mercury Mercruiser
3003 N. Perkins Rd.
Stillwater, OK 74075
(405) 743-6763
Raul Pozner
Institute de Botanica Darwinion
C.c. 22, N1642HYD
Buenos Aires, Argentina
54-11-4743-4800
(Fax)54-11-4747-4748
Paul E. Richardson
1023 South Western Road
Stillwater, OK 74074
(405) 377-4831
Ken Roberts
University of Tulsa
600 South College Ave.
Tulsa, OK 74104
(918) 631-3090
Scott D. Russell
Dept. Botany & Microbiology
770 Van Vleet Oval
University of Oklahoma
Norman, OK 73019-0245
(405) 325-4391
Barbara Safiejko-Mroczka
Dept. of Zoology RH 310
730 Van Vleet Oval
University of Oklahoma
Norman, OK 73019
(405) 325-6192
7
Professional Members 2012-2013
Student Members 2012-2013
Varsha Shah
Texas Woman’s University
P.O. Box 425799
Denton, TX 76204-5799
(940) 898-2366
Dachuan Shi
School of Chemical, Biological
and Materials Engineering
University of Oklahoma
100 E Boyd, SEC, T-335
Norman, OK 73019
(405)708-8940
Reonna Slagell-Gossen
Redlands Community College
1300 S. Country Club Rd.
El Reno, OK 73036
(405) 422-1457
Kent S. Smith
OSU-CHS
1111 W. 17th St.
Tulsa, OK 74107
(918) 561-8246
Gregory Strout
University of Oklahoma
770 Van Vleet Oval
Norman, OK 73019
(405) 325-4391
Phillip Vanlandingham
Dept. of Zoology
University of Oklahoma
730 Van Vleet Oval
Norman, OK 73019
(405) 325-7450
Mike Veldman
Bio Systems Engineering
Room 111 Ag Hall
Oklahoma State University
Stillwater, OK 74078
(405) 744-8392
Mary R. Whitmore
5544 So. Orcas St.
Seattle, WA 98118
(Lifetime Member)
Lisa Whitworth
Oklahoma State University
Microscopy Lab—Venture 1
1110S. Inovation Way
Stillwater, OK 74074
(405) 744-3013
Brittany Bolt
OSU Center for Health Sciences
1111 W 17th Street
Tulsa, OK 74107
Daminda Hemal Dahanayaka
Dept. Physics and Astronomy
University of Oklahoma
440 W. Brooks St., Room 131
Norman, OK 73072
(405) 325-3961 X36564
Felix De La Cruz
University of Oklahoma
865 Asp Avenue, Room 212
Norman, OK 73071
(405) 812-9898
Emanuela Ene
Department of Physics
OSU-Stillwater
Stillwater, OK 74078
(405) 744-2821
Rinosh Joshua Mani
OSU College of Veterinary Health
Sciences
250 McElroy Hall
Stillwater, OK 74078
(405)612-0554
Danny Maples
Oklahoma State University
Deptartment of Chemistry
13 Summit Circle
Stillwater, OK 74045
(405)334-6902
Robert Nicholas
University of Oklahoma
OU ECE Department
1708 Southwest Drive
Norman, OK 73071
Craig Quinalty
University of Oklahoma
1100 Oak Tree Avenue, Apt G2
Norman, OK 73072
(405) 589-0734
Leslie M. Quinalty
University of Oklahoma
Dept. of Chemistry &
Biochemistry
620 Asp Avenue, Room 208
Norman, OK 73019
(405) 325-4811
8
Student Members 2012-2013
Sallie Ruskoski
OSU Center for Health Sciences
1111 W 17th Street
Tulsa, OK 740107-1898
(918)449-6471
Ernest S. Sanchez
University of Oklahoma
Dept. of Physics & Astronomy
440 W Brooks Street
Norman, OK 73019
(405)812-0448
Pranshoo Solanki
University of Oklahoma
334 Carson Engineering Center
202 W. Boyd Street
Norman, OK 73019
(405) 325-9453
J. Byron Sudbury
OSU Graduate Student
P.O. Box 2282
Ponca City, OK 74602-2282
(580) 762-3346
Wesley D. Tennyson
University of Oklahoma
CBME
100 E Boyd, SEC, T-335
Norman, OK 73019
(405)325-3957
Ting Wang
Oklahoma State University
Center for Health Sciences
1111 W 17th Street
Tulsa, OK 74107
(925)998-2512
Zijia Zhang
Oklahoma State University
Center for Health Sciences
111 W. 17th Street
Tulsa, OK 74107
(918) 852-9292
9
Multitasking in Multidimensions: 3D imaging and analysis
Talks include scanning probe microscopy, confocal microscopy,
and 3D optical microscopy. Tours will be available of major mi-
croscopy facilities on campus and entry into the Sam Roberts
Noble Museum of Natural History will be provided.
Upcoming microscopy meetings . . .
Spring Workshop 2013 Stevenson Research and Technology
Center University of Oklahoma
April 12, 2013
Indianapolis Indiana
August 4—August 8, 2013
Hartford Connecticut
August 3 August 7, 2014
OMS Member News
10
Dr. Benjamin Smith has joined the Microscopy Lab at the University of Oklahoma on
January 31, 2013 to manage the confocal microscopy suite, including our newly ac-quired Leica SP8.
Ben has a B.S. in Biotechnology with minors in Biochemistry and Physics (2004) from
the University of Southern Maine. He then went on to get his Ph.D. in Genetics (2012) from the Department of Genome Sciences at the University of Washington. Ben’s thesis research entailed extensive confocal imaging to resolve a long-standing 3-dimensional problem, which was featured in Science (9 November 2012, pp. 798-802).
Ben is now working as a Research Scientist at the Samuel Roberts Noble Microscopy
Lab. He is collaborating on several projects using our Leica SP2, Olympus FluoView 500 and Leica SP8, including projects involving live-imaging, light reflection microsco-py, multi-photon microscopy, and fluorescence correlation spectroscopy.
He also has extensive experience in image rendering, and is assisting with 3D data
presentation on both our Amira and Imaris image rendering platforms.
Ben will also be sharing his experience in an undergraduate/graduate course he will
be teaching this fall on the principles and techniques of light microscopy.
The Samuel Roberts Noble Microscopy Laboratory at the University of Oklahoma welcomes
Dr. Benjamin Smith
OMS News
11
Multi-Photon and FLIM Capable Confocal Microscope at
the Samuel Roberts Noble Microscopy Lab at the
University of Oklahoma is Up and Running
The Microscopy Lab at the University of Oklahoma has recently finished instal-
lation of a confocal laser scanning microscope, the Leica SP8 (see Figure A). The mi-
croscope is equipped with UV optics, a multi-photon laser and two NDD detectors,
FLIM with single photon detection via avalanche photodiodes, hybrid HyD detectors for
increased sensitivity, a heated stage, and a resonance scanner, making it an all
around general purpose instrument.
As part of the purchase, OU also purchased the Imaris® rendering software
from Bitplane®. In addition to rendering both 3D images (see Figures C and D) and
movies for presentations, the software can also handle any confocal file format and
can also render XYZT samples. The software also comes with an extensive image
analysis package, including and axon/dendrite arboration tracker, 3D distance and vol-
umetric measurement, particle tracking in XYZT series, and a powerful statistical anal-
ysis suite powered through MatLab. The powerful computer running all this is cutting-
edge as well, and allows for rapid rendering and analysis of data sets even gigabytes
in size.
Figure (A) Leica SP8 confocal microscope installed at OU. (B) A shadow rendering via Imaris® of a section of Lily Root that was im-aged using the multi-photon laser. (C) An isosurface rendering of the same sample at an oblique view
A
B C
12
OMS Ugly Bug Scope Deliveries
Teacher Angie Brown and student at Monroe Elementary.
Community Christian students pre-pare for their first look through their
new microscope .
Charlotte with teacher Mrs. Baustert and her 4th grade class after presenting them with their mi-croscope as winners of the Ugly Bug Contest.
Teacher Brian Simmons with student who collected Commerce Middle
School’s winning Ugly Bug.
Preston awards bug collector at Jeffer-son Elementary her winning bug photo.
OMS Ugly Bug Scope Deliveries
13
Yes! The Hitachi TM3000 portable SEM does get taken out even in the rain!
Preston with the Ugly Bug collector at Community
Christian School.
Ernie talks to students at Jefferson Elementary about using the electron
microscope.
A bottle of water does act like a lens!.
Students at Kelley elementary get a look at bugs before they go into the SEM
OMS Annual Spring Meeting
14
15
Register online at www.regonline.com/oms
OMS Spring Meeting Registration
17
18
Direction to Spring Meeting
The workshop venue is directly North from the National Weather Center.
Take highway 9 to Jenkins Ave. and turn North. The SRTC is shown as
#6 in the map below. Parking lots are located to the east of the SRTC (see map on
next page).
19
Direction to Spring Meeting
OMS meeting attendees can park in lots designated for research campus
parking directly east of the SRTC. It is not necessary to park in a designated visitor spot.
Overlflow parking in the event that parking spaces are not available directly adjacent to
the SRTC can be accommodated in the Lloyd Noble Center arena lot across Jenkins Ave.
20
Direction to Spring Meeting
21
OMS Spring Meeting Abstracts
Keynote Talk
Manipulation and nanotribology in dry and liquid environ-
ments and nanomechanics of nano-objects with using an AFM
Dave Maharaj and Bharat Bhushan
†
Nanoprobe Laboratory for Bio- & Nanotechnology and Biomimetics (NLBB) The Ohio State University, 201 W.19
th Avenue
Columbus, Ohio 43210-1142, USA †[email protected] http://www.mecheng.osu.edu/nlbb/
Nano-objects have been investigated for applications that require controlled ma-nipulation and targeting in biomedicine and the oil industry. Applications include, but are not limited to, their use in targeted drug delivery and chemical sensors in the identification of oil, removal of contaminants and enhanced oil recovery. In the-se applications, low friction in liquid medium is of interest. Manipulation studies with an atomic force microscope (AFM) submerged in low viscosity liquid environments have shown reductions in friction when compared to dry environments
1-4. Studies
were carried out in single-nano-object contact where nano-objects were pushed lat-erally using an AFM tip. These studies provide understanding of the nature of the friction mechanism
1-4.
In tribological applications on the micro/nano-scale, the initial start-up forces and torques needed become high, which can hinder device operation and reliability. The choice of a suitable lubricant on these scales becomes crucial
5-6. Reductions
in friction and wear have been shown to occur when spherical and tubular nano-objects were introduced as solid lubricants in dry and submerged in low viscosity liquid environments
1-4. Studies were carried out in multiple-nano-object contact us-
ing an AFM. These studies simulate the ensuing contacts experienced when nano-objects were introduced for the purpose of friction and wear reduction
1-4.
Nano-objects are also being used in various nanotechnology applications which are dependent on their mechanical properties. Generally, as the size decreases there is an increase in the mechanical properties of nano-objects. Results in the lab have shown this scale dependence with hardness for gold (Au) nanoparticles as well as a reduction in hardness below a critical size. Understanding and character-izing the mechanical properties of nano-objects is crucial to their continued use.
22
OMS Spring Meeting Abstracts
Keynote Talk
Nanomanipulation studies of nano-objects in dry and liquid environments will aid in better design of applications requiring controlled manipulation and targeting of nano-objects. Nanotribological studies are useful for applications requiring low fric-tion and wear. Nanomechanics studies provides fundamental understanding of the nature of mechanical properties and helps connect the macroscale to the micro/nano-scale. This talk will present research in nanomanipulation and nanotribologi-cal studies in dry and submerged in liquid envirionments and fundamental studies of mechanical properties of nano-objects.
1 Maharaj, D. and Bhushan, B. (2012), ―Effect of spherical Au nanoparticles on nanofric-
tion and wear reduction in dry and liquid environments,‖ Beilstein J. Nanotechnol. 3, 759-772. 2 Maharaj, D. and Bhushan, B. (2013), ―Effect of MoS2 and WS2 nanotubes on nanofriction
and wear reduction in dry and liquid environments,‖ Tribol. Lett. 49, 323-339. 3 Maharaj, D. and Bhushan, B. (2013), ―Effect of Carbon Nanohorns on nanofriction and
wear reduction in dry and liquid environments,‖ J. Colloid Interface Sci. doi:10.1016/j.bbr.2011.03.031 4
Palacio, M. and Bhushan, B. (2008), ―A nanoscale friction investigation during manipula-tion of nanoparticles in controlled environments,‖ Nanotechnology, 19, 315710 5 Bhushan, B., Springer Handbook of Nanotechnology, 3
rd ed., Springer, Heidelberg, Ger-
many, 2010. 6
Bhushan, B., Nanotribology and Nanomechanics I & II, 3rd
ed., Springer, Heidelberg, Germany, 2011.
23
OMS Spring Meeting Abstracts
Manipulation and nanotribology in dry and liquid environ-
ments and nanomechanics of nano-objects with using an
AFM
Dave Maharaj and Bharat Bhushan†
Nanoprobe Laboratory for Bio- & Nanotechnology and Biomimetics (NLBB) The Ohio State University, 201 W.19
th Avenue
Columbus, Ohio 43210-1142, USA †[email protected] http://www.mecheng.osu.edu/nlbb/
Bio Dave Maharaj is a Ph.D. candidate in Mechanical Engineering at The Ohio State Uni-versity, where he is a member of the Bhushan research group. He completed B.E. and M.E. degrees at the City College of the City University of New York. His primary research interests involve the application of AFM and SEM to investigations of tribolo-gy, nanoindentation, and the links between nano- and macro- scale friction and wear. To date, his work has resulted in three publications: Maharaj, D. and Bhushan, B. (2012), ―Effect of spherical Au nanoparticles on nanofric-
tion and wear reduction in dry and liquid environments,‖ Beilstein J. Nanotechnol. 3, 759-772.
Maharaj, D. and Bhushan, B. (2013), ―Effect of MoS2 and WS2 nanotubes on nanofric-tion and wear reduction in dry and liquid environments,‖ Tribol. Lett. 49, 323-339.
Maharaj, D. and Bhushan, B. (2013), ―Effect of Carbon Nanohorns on nanofriction and wear reduction in dry and liquid environments,‖ J. Colloid Interface Sci. doi:10.1016/j.bbr.2011.03.031
The Bhushan group is widely recognized as world leaders in the application of micros-copy to investigate issues of friction and wear and other nanotechnology applications. Over the last year, Dr. Bhushan and group members have authored and edited the fol-lowing published books: Introduction to Tribology, 2nd Edition (Wiley), Principles and Applications of Tribology, 2nd Edition (Wiley), Biomimetics: Bioinspired Hierarchical-Structured Surfaces for Green Science and Technology (Springer), Nanotribology and Nanomechanics – An Introduction, 3rd Edition (Springer), and Encyclopedia of Nano-technology (Springer).
24
OMS Spring Meeting Abstracts
Quantitative nanomechanical mapping with AFM
Dr. Senli Guo Bruker Nano Surfaces Division
Bio Dr. Senli Guo
Bruker Nano Application Scientist 2011-present
Oak Ridge National LabCenter for Nanophase Materials Sciences (CNMS) 2009-2011 Dr. Sergei Kalinin’s Nanoscale Functional Imaging Group, Developed methodologies to study the kinetics of force-induced conformation transition of
polymer molecules by combining the band excitation approach to the AFM based single molecule force spectroscopy.
Applied Electrochemical Strain Microscopy (ESM) to study lithium ion diffusion in battery cathode materials and near battery cathode-electrolytes interfaces to help develop bet-ter lithium ion battery.
Developed methodologies of functional recognition imaging by combining the force-volume technique to the Band Excitation Piezoresponse Force Microscopy (PFM).
Developed a dual tapping mode by combining the high mode resonant frequencies and applied this technique to characterize the mechanical, chemical and physical properties of a variety of polymer materials, such as polymer diamond composites and battery ma-terials (Nafion, Celgard, lithium salt polymer electrolytes).
Duke University Biophysical Chemistry 2004-2009 Dr. Boris Akhremitchev’s Group, Research Assistant Applied the AFM-based single molecule force spectroscopy to study the interactions be-
tween individual molecules, including protein-protein interactions, ligand-receptor inter-actions, hydrophobic interactions and the electrostatic interactions between the like-charged nanoparticles. The kinetic and thermodynamic parameters of the energy land-scape of interactions can be extracted from our results and help understand the effects of these interactions in many biological and technological processes. Moreover, a new model has been developed to improve the data analysis of single molecule force spec-troscopy measurements.
Applied the AFM-based nanoindentation and imaging techniques to investigate the mecha-nisms of protein amyloid aggregation and characterize the mechanical properties of am-yloid fibrils. The original data analysis approach applied in the AFM nanoindentation method has been improved. The AFM nanoindentation method has been extended to the study of mechanical properties of other biological aggregate systems, such as insu-lin protein crystals, retinal pigments.
Investigated the morphology and mechanical properties of human retinal pigment in collab-oration with Dr. Simon John (Duke Chemistry Department).
25
2011 OMS Spring Workshop
Article I. NAME
The name of this organization shall be the Oklahoma Microscopy Society. The acronym
shall be OMS. OMS is a non-profit organization.
Article II. PURPOSE
The purpose of OMS shall be the advancement of the science of microscopy in Oklahoma
and nationally by:
encouraging the dissemination of knowledge of microscopy including its technology and
instrumentation.
promoting the free exchange of ideas and data among interested individuals and
encouraging interdisciplinary interaction between microscopists.
Article III. MEMBERSHIP
Section 1. Types:
Regular membership shall be open to any person who has an interest in
microscopy.
Corporate membership shall be open to any commercial or non-profit
organization that has an interest in microscopy. A member organization may
designate one representative to receive all privileges of membership. Other
members of the same organization may become regular members.
Honorary membership may be given to a person named an Honorary
member by vote of the Executive Committee.
Section 2. Enrollment: Any eligible person or organization may make application for mem-
bership to the Executive Committee of OMS. Completed application forms shall be submit-
ted to the Secretary-Treasurer of OMS with one year’s dues.
Section 3. Privileges: All members have the right to vote at any business meetings held by
OMS and to hold elective office.
Constitution & Bylaws of the OMS
26
Section 4. Dues:
Annual dues shall be five dollars for Regular membership for students, fifteen dollars
for Regular membership for non-students, and fifty dollars for Corporate mem-
bership.
Dues shall become payable on July 1 of each year for the following twelve months.
Any member that is delinquent in payment of dues for a period of six months shall be
dropped from membership. Members thus dropped may be reinstated thereafter
by paying one year’s delinquent dues and the current year’s dues.
Article IV. MEETINGS
At least one business meeting per year shall be held. The time(s) and place(s) of such meet-
ings shall be designated by the Executive Committee and duly announced. Business meetings
shall be conducted according to Robert’s Rules of Order.
Article V. OFFICERS
Section 1. The officers of OMS shall be a President, a President-Elect, a Secretary-Treasurer,
a Member-at Large for Biological Sciences, a Member-at Large for Physical Sciences, and a
Member-at Large for student members. These officers shall perform the duties prescribed by
these bylaws and by the parliamentary authority adopted by the Society.
Section 2. Duties:
a. The President shall preside at all meetings of the Executive Committee and busi-
ness meetings of the OMS and promote the interests of OMS both within the
state and nationally.
b. The President-Elect shall assist the President, substitute for him/her
when necessary, perform any duties assigned by the President and be responsible
for organizing the regular spring workshop/seminar.
c. The Secretary-Treasurer shall maintain records of OMS and
communicate with members. This officer shall be custodian of OMS funds, col-
lect all dues, notify members delinquent in membership and account for OMS
funds in accordance with accepted business practice.
d. Members-at-Large shall represent their respective constituents.
Constitution & Bylaws of the OMS
27
Section 3. Term of Office:
The President, President-Elect, and Members-at-Large shall each serve for one year
beginning July 1 and ending June 30 of the following year.
The Secretary-Treasurer shall serve for two consecutive years beginning July 1 and
ending July 30 of the second following year.
Section 4. Election: Officers shall be elected as prescribed in Article VII of these bylaws.
Section 5. Vacancies: If the President cannot serve, the President-Elect shall immediately suc-
ceed to that office. If the President-Elect or any other officer cannot serve for any reason, the
Executive Committee shall appoint a person to serve pro tem in the vacant office. Any such
appointed officer shall be replaced by one duly elected at the next annual election in May.
Article VI. EXECUTIVE COMMITTEE
Section 1. Composition: The Executive Committee shall consist of the officers of OMS, plus
the Newsletter Editor ex officio who shall be without vote.
Section 2. Duties:
The Executive Committee shall conduct the business of OMS as specified herein and
otherwise as necessary, and shall advise the membership on matters concerning
the management of OMS. It shall appoint the Newsletter Editor.
The Executive Committee shall hold not fewer than two meetings annually, on call of
the President or a majority of its members.
Article VII. ELECTIONS
Section 1. Nominations of officers except the President shall be made by a nominating Com-
mittee appointed by the President and approved by the Executive Committee. This Committee
shall consist of five persons, at least one of whom is from the field of Biological Sciences and
one from the field of Physical Sciences. Nominations may be solicited from the membership
at any time.
Section 2. The Nominating Committee shall present a slate of consenting candidates (two for
each office) to the President prior to the spring general business meeting. The President and
Secretary-Treasurer shall announce this list to the membership at the spring general business
meeting. Additional nominations of persons willing to serve may be solicited from the floor at
this time.
Constitution & Bylaws of the OMS
28
Section 3. The Secretary-Treasurer shall prepare and mail ballots to all members by May 15
and shall accept ballots until May 31.
Section 4. Ballots shall be counted by at least two Executive Committee members and may be
reviewed by the entire board if deemed necessary. In each case the candidate receiving the
largest number of votes shall be declared elected. Any tie shall be resolved by vote of the
combined Executive and Nominating Committees. Results shall be announced by the Secre-
tary-Treasurer at the next business meeting or by mail to all members.
Article VIII. AD HOC COMMITTEE
The President shall appoint ad hoc committees as necessary or helpful in managing affairs of
OMS. Committee members shall be considered automatically discharged at the end of the
appointing President’s term of office unless the new President specifically requests that they
continue. The committee itself shall continue until its purpose has been fulfilled or it is dis-
solved by vote of the executive board or the membership at large.
Article IX. AMENDMENTS
Section 1. Amendments may be suggested at any OMS business meeting. However, amend-
ments to these bylaws may be formally proposed in only two methods:
By the Executive Committee or
By petition of ten percent of the members.
Section 2. The proposed amendment shall then be promptly submitted by mail to the member-
ship by the Secretary-Treasurer, along with the signed statement of reasons for support and/or
opposition. Returned ballots shall be accepted by the Secretary-Treasurer for three weeks after
the date of mailing. The Executive Committee shall count the ballots and the amendment(s)
shall be declared ratified if a two-thirds majority of the votes cast is favorable.
Section 3. Any member who so desires may be present at the counting of such ballots.
Article X. DISSOLUTION
In the event of the dissolution of the OMS, upon the discharge of all its debts and obligations,
any remaining assets shall be given to such tax-exempt scientific organization as the Executive
Committee may determine. In no case shall any assets be used for the direct benefit of any
member of OMS.
Constitution & Bylaws of the OMS
29
30
Oklahoma Microscopy Society Membership Application/ Renewal Form Name: _____________________________________ Business Phone: _____________________________________ FAX: _____________________________________ Email: _____________________________________ Institution: _____________________________________ Address: _____________________________________
_____________________________________
_____________________________________
Check here if Address is New/Revised: ___ Membership in Affiliated Societies: Microscopy Interests:
MSA Physical Sciences MAS Biological Sciences_____ OAS Other
Membership Dues: Type:
Corporate ($50.00)_____ Professional ($15.00)_____ Student ($5.00)_____
Amount Enclosed:_____
Please enclose a check for one year’s dues (July 1, 2012 - June 30, 2013) made out to: “OMS” or “Oklahoma Microscopy Society” and mail to:
Bill Meek, Secretary/Treasurer, OMS Dept. of Anatomy & Cell Bio. College of Osteopathic Medicine Oklahoma State University 1111 W. 17
th Street
Tulsa, OK 74107 Email: [email protected]
We thank the following
for their support
of the Spring 2013 Newsletter
FEI Company
Diatome Diamond Knives
Electron Microscopy Sciences
Leica Microsystems Inc.
Ted Pella
Tousimis
31
