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Assembly and Deformation of the Bacterial Cell Wall

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Assembly and Deformation of the Bacterial Cell Wall. Rico Rojas Huang and Theriot Labs. Cell Morphogenesis – The cell wall defines cell shape. Spirochete Bacteria. Micrasterias. Phycomyces. Acetabularia. Pollen tube growth. Pulsatile pollen tube growth. - PowerPoint PPT Presentation

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mbly and Deformation of the Bacterial Cell Rico Rojas Huang and Theriot Labs

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Page 1: Assembly and Deformation of the Bacterial Cell Wall

Assembly and Deformation of the Bacterial Cell Wall

Rico Rojas

Huang and Theriot Labs

Page 2: Assembly and Deformation of the Bacterial Cell Wall

MicrasteriasSpirochete Bacteria

PhycomycesAcetabularia

Cell Morphogenesis – The cell wall defines cell shape

Page 3: Assembly and Deformation of the Bacterial Cell Wall

Pollen tube growth

Page 4: Assembly and Deformation of the Bacterial Cell Wall

Page 5: Assembly and Deformation of the Bacterial Cell Wall

Pulsatile pollen tube growth

Page 6: Assembly and Deformation of the Bacterial Cell Wall

Page 7: Assembly and Deformation of the Bacterial Cell Wall

Actin homologs orchestrate cell wall assembly in bacteria

Jones 2001

Page 8: Assembly and Deformation of the Bacterial Cell Wall

Research Agenda

1. What is the growth rate dependence on turgor pressure?2. What determines MreB localization?

Page 9: Assembly and Deformation of the Bacterial Cell Wall

What is the growth rate dependence on turgor pressure – varying the turgor pressure

Page 10: Assembly and Deformation of the Bacterial Cell Wall

Furchgott 2011

Growth rate dependence on turgor pressure – computational framework

Page 11: Assembly and Deformation of the Bacterial Cell Wall

MreB Localization – MreB binds ATP and mutations around the nucleotide binding pocket affect MreB localization and cell shape in Caulobacter

Dye 2011

Page 12: Assembly and Deformation of the Bacterial Cell Wall

Using MreB mutants as a perturbation to study localization

E. coliC. crescentus

WT

D189G

A325P

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