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Atomic Force Microscopy (AFM) of Neurons

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Project: AFM test towards investigation of neurons and neuronal microtubules.
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Home Page Title Page Contents JJ II J I Page 1 of 15 Go Back Full Screen Close Quit Project: AFM test towards investigation of neurons and neuronal microtubules Presented in PosLab (C368) on May 30 th , 2007, at 15:15 by Mikael O. Bonnier for the course Experimental Biophysics, TNF030 with advisor Lars Henrik Dæhli Skjolding
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Page 1: Atomic Force Microscopy (AFM) of Neurons

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Project: AFM testtowards investigation

of neurons andneuronal microtubules

Presented in PosLab (C368) on May 30th, 2007, at 15:15

by Mikael O. Bonnier

for the course Experimental Biophysics, TNF030with advisor Lars Henrik Dæhli Skjolding

Page 2: Atomic Force Microscopy (AFM) of Neurons

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Chip with interdigitated electrodes, but these are pictures of thechip connections in the micrometer range in optical and AFMheight, respectively.

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Three contact mode cantilevers, a single intermittent contactmode cantilever, and three intermittent contact mode can-tilevers, respectively on one chip each.

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Adjusting laser beam and detector.

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QuitAFM image using worn tip. Note the missing data.

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The advanced optical microscope (Nikon TE2000-U) with theAFM mounted.

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Active vibration damper (Halcyonics Micro 40).

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Images of the marker pen cross in water using opticalmicroscope and AFM, respectively. Note the missing data.

Page 9: Atomic Force Microscopy (AFM) of Neurons

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BioCellTM.

Page 10: Atomic Force Microscopy (AFM) of Neurons

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Stem cells using x40 microscope lens.

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Invading bacteria using x10 microscope lens.

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More differentiated stem cells in optical directly overlayed withAFM height images (by Dr Rachel Owen at JPK Intruments

demonstrating the AFM in our lab).

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Conclusions

• Study the manuals and control theory

• Better if prism were made so that it could only fit one wayinto the AFM

• Many interesting experiments, that may require stem cells ge-netically modified so that they have different microtubule hairlengths, can be made using our new AFM

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Acknowledgements

• Lars Henrik Dæhli Skjolding

• Jan Tønnesen

• Jonas Tegenfeldt

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QuitHappy Summer!


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