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8/13/2019 Bacterial Diversity in Tap Drinking Water
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IN
TAP DRINKING WATERPresented by:
Tongbram Usharani Devi
M Sc.
Under the Supervision:
Dr. Indu Sharma
Department of Microbiology, Assam University, Silchar
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Introduction
Water is an indispensable
element in the maintenance
of all life form on Earth .
Adequate supply of safe
drinking water is
prerequisites for a healthy
life, but waterborne disease
is a major cause of death in
many parts of the world.
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Factors for water contamination:
Biologically- Microbes like viruses, protozoa or bacteriaPhysically- Thermal pollution
Chemically- Domestic waste, Agricultural, Municipal waste
etc.
Biological Contaminants:
Drinking water must be free from pathogens like viruses,
protozoa or bacteria which may cause diseases such as
hepatitis, giardiasis, typhoid, paratyphoid, dysentery,
cholera and many others.
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Coliform Bacteria
Enterobacteriaceae, gram-negative, non-spore forming,
oxidase-negative, rod-shaped facultative anaerobic
bacteria that ferment lactose to acid and gas within 24-48
hours at 36 ±2 ̊ c.
A potential indicator for the presence of disease-causing
organisms may be in tap water. Health Effects:
Harmless, but presence is a sign for faecal pathogens, includingnon-coliform pathogens such as other forms of bacteria, viruses,
or protozoa.
It may causes diarrhoea, cramps, nausea, jaundice, headaches,
fatigue and so on
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OBJECTIVES
Isolation of coliform organisms from tap
drinking water.
Biochemical characterization of isolated
organism for identification.
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Biochemical tests
Catalase test- (Bubble formation with Hydrogenperoxide)
Oxidase test- (Blue colour with oxidase disc)
Indole test- (Red Violet ring in Kovac’s reagent afterculturing in peptone water)
Methyl red test- (Red colour in Methyl red reagentafter culturing in peptone water)
Vogas-proskauer (VP) test- (Pink colour with VP
reagent I and II) Citrate test- (Blue colour in Simmon Citrate agar slant)
Triple sugar iron (TSI) test.
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BIOCHEMICAL TEST
TSI and Citrate Test MR Test
Microscopic
View: E.col i
Microscopic
View: Klebsiel la
sp.
Colony
Count
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The Biochemicaltest results confirm
the presence of
E.coli and Klebsiel la
sp. in the drinking
Tap water samples
collected and
shows thepercentage of
isolates.
52%
48%
Chart Title
E.Coli Klebsiella
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Discussion
In this study, the results showed that most testsamples were found the presence of coliformsbacteria in different CFU. MassCommunication
canteen’s water got highest
coliforms (700 CFU) than other samples. So,most of the University canteen’s water werecontaminated with coliform organisms andmay be health risk to the consumer.
Out of twenty seven isolates,14 (E. coli) and13 (Klebsiella sp)were detected by the help ofbiochemical tests.
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Conclusions
Out of twenty seven isolates, 14(E.coli ) and
13(Klebsiella sp) were found in the drinking tap
water.
These indicated that the proper measurementshould be taken or should be check carefully.
No single parameter can fill all the information
needs. So, Different parameters like source water
quality assessment, assessment of treatmentefficiency, monitored the quality of drinking water
leaving the treatment facility and in the distribution
system.
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THANK YOUFor your kind attention