Vitamin c concentration available in market juices

VITAMIN C CONCENTRATION IN DIFFERENT ORANGE JUICE BRANDSINTRODUCTION Vitamin c also known as ascorbic acid is one of the most effective nutrients. Benefits vitamin c may include protection against immune system deficiency, cardiovascular disease, prenatal health problems eye disease and even skin wrinkling. Vitamin c is a water soluble vitamin meaning it is not stored in our bodies. We have to get vitamin c from foods like citrus fruits, broccoli, and tomatoes. Vitamin C reacts with DCPIP in the following manner:DCPIP (blue) + H+ DCPIPH (pink)DCPIPH (pink) + VitC DCPIPH2 (colorless)

Overall Reaction:C6H8O6+ C12H7NCl2O2C6H6O6+ C12H9NCl2O2Ascorbic acid + HDCPIP dehydroascorbic acid + DCPIPH2

VARIABLESIndependent Variable Different orange fruit juices Dependent Variable Titre value of fruit juicesControlled Variable Concentration and amount of DCPIP

OUR EXPERIMENTAIMTo compare the vitamin C content in:Home-made squeezed orange juice Realorange fruit juice,Tropicana- orange fruit juice, andMinute maid pulpy orange juice.

Thereby determining which of the above is best for consumption of Vitamin C.

Hypothesis The vitamin c content would be the greatest in the homemade orange juice from orange fruit when compared to market juices. Materials UsedContainers to collect small amount of juicesMeasuring cylinder Pipette to measure 2 ml volumePipette to accurately measure volume up to1 mlTest tubes (5)SpatulaDistilled water Vitamin C (0.1% fresh: 0.1% = 1mg /ml)DCPIP dye (0.01% aqueous solution fresh)

ProcedureWash all the apparatus thoroughly with water.Prepare orange juice from orange fruit without adding any water to it.Take 2ml of 0.01% DCPIP solution in a testube. Take juice in 1ml pipette and titrate it against 2ml DCPIP solution.Shake the tube gently after the addition of each drop and continue to add drops until the DCPIP solution is decolorized.Repeat this process for all other juices.PRECAUTIONSThe titration has to be drop by drop as the solution may change within the addition of amounts as small as two drops.The concentration of the DCPIP has to be kept constant, so the amount of dye to be added has to be of precise valueThe apparatus has to be clean or else the same apparatus shouldnt be used for keeping the juices because it wont give the correct readings.RAW DATAFreshly Squeezed Orange Fruit Extract

10.04ml 20.05ml30.05mlAverage0.04ml

ResultThe colour change after titarionReal Orange Juice

10.34ml 20.26 ml30.26mlAverage0.28ml

Result

The colour change after titrationTropicana Orange Juice

1 0.33 2 0.35 3 0.35 Average 0.34

result

Colour change after titrationMinute Maid Pulpy Orange Juice

1 0.55ml 2 0.60ml3 0.60mlAverage 0.58ml

ResultColour change after titration

0.1% Ascorbic Acid

1 0.06ml2 0.04ml 3 0.04mlAverage 0.04mlResult

Colour Change after TitrationDATA PROCESSINGGraph showing vitamin c concentration22Error graphsrhmtropicana realminute maidas.acid10.040.330.340.550.0620.050.350.260.60.0430.050.350.260.60.04hmtropicanarealminute maidas.acidav0.0460.3430.280.580.04max-mean0.010.010.060.020.02mean-min0.0060.0130.020.030srHomemade Tropicana 10.040.3320.050.3530.050.35Homemade Tropicana av0.040.34stdev0.0057740.011547sr no.hmminute maid10.040.5520.050.630.050.6averagehmmm0.040.58stdev0.010.03Std. deviation for Freshly Squeezed juice & Tropicana sr no.hmreal10.040.3420.050.2630.050.26juicesHome maderealaverage0.040.28stdev0.010.0462LIMITATIONSThe most difficult part of the experiment was detecting the change in colour of the DCPIP solution while performing the titration. Two of the juices showed clear disappearance of the colour, while the others had the tint of yellow due to some other compounds of colours present. The date of manufacture may be different which may change the nutrient content or concentration in different juices.

Conclusion From our experiment we thus conclude that homemade juice has the most vitamin c concentration when compared to available market juices.THANK YOUCompiled & Presented By: Vibhuti Sapariya Niyati Bamrolia