BIOCHEM Cairo 2013 Abstract Book

The First Annual Meeting of Medical Biochemistry & Molecular Biology Department, Cairo University, 30 March, 2013

BIOCHEM CAIRO 2013 Abstract Book

Pag

e1



Pag

e2

BIOCHEM Cairo 2013 The First Annual Meeting of the

Medical Biochemistry and Molecular Biology Department

Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt

Organized under the Patronage of

Prof. Hosam Mohamed Kamel, President of Cairo University

Prof. Hussein Mahmoud Khairy, Dean of Kasr Al-Ainy Faculty of Medicine

Prof. Yasser Hussien Nassar, Head of Medical Biochemistry and Molecular Biology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University

BIOCHEM Cairo 2013 Honorary Chairs

Prof. Mohamed Talaat Abd-El Aziz Prof. Mohamed Rasheed Bahgat

BIOCHEM Cairo 2013 Chairman

Prof. Mohamed Aly Abdel-Hafez

Secretary General

Prof. Mohamed Abdel-Aziz Wasef



Pag

e3

BIOCHEM Cairo 2013 Advisory Board

Prof. Bothayna M AlKamah Prof. Locie S Yousof Prof. Soliman A Al-Safoury Prof. Zeinab H El-Kirdasy Prof. Kawthar H Fahmy

Scientific Committee

Prof. Gamil Amin Tawedreous Prof. Nahed A Abdelghani Prof. Olfat G Shaker Prof. Hanan Hassan Fouad Prof. Hanan Hosny Ahmed Prof. Laila Ahmed Rashed Ass.Prof. Dina Sabry Abdel Fatah Ass.Prof. Fatma Mohamed Taha

Organizing Committee

Prof. Dawlat El-Sayed Salem Ass.Prof. Eman Mohamed Obaya Ass.Prof. Amira Ahmed Hassouna Dr. AbdelKarim Gamal Kamel Dr. Doaa Moustafa Gharib Dr. Goerge Nazeih Beshara Dr. Moustafa Mohamed Moustafa Dr. Rania El-Sayed Hussein

Venue & Date

Learning Resource Center, Kasr Al-Ainy School of Medicine, Cairo University

30 March, 2013

Meeting Website

www.medbiocairo.com

http://www.medbiocairo.com/



Pag

e4

Acknowledgments

BIOCHEM Cairo 2013 leadership and committees wishes to express their appreciation to: Professor Hussein Mahmoud Khairy, Dean of Kasr Al-Ainy Faculty of Medicine and Professor Hesham El Saket, Director LRC , Kasr Al Ainy Faculty of Medicine, Cairo University for their generous support to the Meeting.

BIOCHEM Cairo 2013 Guest of Honor

BIOCHEM Cairo 2013 leadership welcomes Dr. Abdalla Al Najjar , President of The Arab Science and Technology Foundation (ASTF) as the meeting guest of honor

BIOCHEM Cairo 2013 Exhibition

BIOCHEM Cairo 2013 leadership and committees express their appreciation to the meeting sponsors and exhibitors

Platinum Sponsor

El Mehy Engineering

Golden Sponsors

AccuVis Bio

Clinilab

Gene Tech

Silver Sponsor

Gamma Trade



Pag

e5

Welcome Message

Dear friends and colleagues:

Welcome to BIOCHEM Cairo 2013!

It is with great pleasure and honor we welcome you in the First Annual Meeting of the

Medical Biochemistry and Molecular Biology Department, Kasr Al-Ainy School of Medicine,

Cairo University

Themed “Molecular Biochemistry in Clinical Practice”, this Meeting represents a converging

point to all key specialists from schools of medicine and pharmacy to highlight recent

advances in our field that heralds advances in clinical practice. It also celebrates sixty years

of the discovery of the double helix in 1953 and ten years of the completion of the Human

Genome Project in 2003. These are the two events which are still inspiring our research in

our ever developing challenging field.

As a participant of this event, you will receive the latest knowledge in Medical Biochemistry

and Molecular Biology through plenary lectures, presentations as well as workshops.

This meeting would not have been possible without the support of Kasr Al-Ainy School of

Medicine leadership and the cooperation of the Learning Resource Center (LRC), Kasr Al

Ainy. I am also indebted to the efforts of my colleagues at the Department.

For all of them I am very grateful.

I wish you all a fruitful event

Sincerely

Professor Dr. Mohamed Aly Abdelhafez

Chairman of BIOCHEM CAIRO 2013

http://medbiocairo.com/biochemcairo2013plenary-lectures/

http://medbiocairo.com/biochemcairo2013-workshops/



Pag

e6

Scientific Program

Hall B ( 1st Floor) Main Hall A ( 2nd Floor) 30 March, 2013

Registration 09:00-10:00

Welcome Messages 10:00-11:00

Plenary Lectures

Chairpersons: Prof. Ahmed Abbas Raouf, Prof. Maysa Kamal Salama, Prof. Dawlat El Miligy

Micro RNA Signature in Human Cancer,

Professor Olfat G Shaker

Personalized Medicine: Role of Molecular

Genetic Profile in Anticipation, Diagnosis and Management of Disease, Prof. Hanan H

Fouad.

Key Note Lecture

How to Link the Research Centers and Academic Units with the Economic and industrial Sector?, The Arab Science and Technology Foundation (ASTF) Approach, Dr. Abdallah Al Najjar, President ASTF, UAE

11:00-12:15

Coffee Break/ Poster Review & Exhibition 12:15-13:00

Accepted Oral Presentations (First Session) Chairpersons: Prof. Mahmoud Zahran, Prof. Emad Zaki Abbas, Prof. Hanan AbdelAziz Ibrahim

1- Role of EPCs in Collaterals development for Patients with Chronic Total Coronary Artery Occlusion and Neovascularisation Development in an Experimental Model with AMI, Dr. Doaa Gharib, Egypt

2-Activin A/Follistatin Mage-1 and 3 Transcripts: Markers for Hepatocellular Carcinoma, Dr. Sabah

Abdelhady Mahmoud, Egypt

3- Lipoprotein-associated 3-Phospholipase A2 Levels

as a Predictor of Cardiovascular Risks in Patients with COPD and Obstructive Sleep Apnea, Dr. Eman Abd El-

Fatah, Egypt

13:00-14:00



Pag

e7

4-The Association Between Cyclooxygenase 2 Gene

( rs 20417) Polymorphism and Coronary Artery

Disease at Suez Canal University Hospital in Ismalia, Dr. Amany Youssef El Kazaz, Egypt

HALL B HALL A

Break 14:00:14:15

Industrial Session: Clinical Chromatography

Chairpersons: Prof. Soliman Al-Safoury, Prof. Fatma Ahmed Ayad, Prof. Aisha Eid Saleh

Department Scientific activities:

1- MSc thesis: Single Nucleotide

Polymorphism of IL- 10 and IL- 28 B as Predictors to the

Response of Interferon Therapy in HCV Infected Children,

Candidate: Zeinab Ahmed

Mohamed Nour Attia

2- Student topic: Retinoplastoma from Statistical Inheritance to Epigenetics, Miss. Yousra Elshoura

Accepted Oral Presentations (Second Session)

Chairpersons: Prof.Taheya Hashem Selim, Prof. Mona Mohamed Al Zain , Prof. Manal Mohsen Shaltout

5-Apo CIII 3238C/G Gene Polymorphism Influences

Oxidized Low-density Lipoprotein with a Risk of Essential Hypertension among Egyptians, Dr. Maivel

Ghattas, Egypt

6-Lack of Association between the -2548G/A Polymorphism of the Leptin Gene and Hypertension in

Saudi Adult Patients, Dr. Amany Osama , Egypt

7-Comparison between Serum Levels of Retinol

Binding Protein-4 and Total Thiols in Generalized Obesity and Abdominal Obesity Regarding

Cardiovascular Risks, Dr. Naglaa Kamal Idriss, Egypt

8-Mesenchymal Stem Cells and a Curcumin Derivative on Notch Signaling in Hepatocellular Carcinoma. Dr. Walaa Ibrahim Ali Ahmed

14:15-15:15

Closing Remarks 15:15-15:30



Pag

e8

Plenary Lectures



Pag

e9

Micro RNA Signature in Human Cancer

Olfat Shaker, Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt

MicroRNAs are short 20-22 nucleotide RNA molecules that function as negative regulators of gene expression in eukaryotic organisms. RNA mediated gene silencing pathways have essential roles in development, cell differentiation, cell proliferation, cell death, chromosome structure and virus resistance. MicroRNA molecules are produced from larger transcripts that form hairpin precursors. Two RNase III endonucleases, Drosha and Dicer, cut both strands of the hairpin and generate ~22 nucleotide microRNA duplex.

MicroRNA alterations are involved in the initiation and progression of human cancer. The causes of the widespread differential expression of microRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the microRNA processing machinery.

There has been demonstrated a possibility to use microRNA signatures for a specific cancer classification with potential predictive and therapeutic value. MicroRNAs are aberrantly expressed in all studied cancer tissues, are located in cancer-associated genomic regions and their putative targets are very often tumor suppressors or oncogenes. The known data provide an evidence that microRNAs could disclose new ways for cancer diagnosis, prognosis estimation, and therapy.



Pag

e1

0

Personalized Medicine: Role of Molecular Genetic Profile in Anticipation, Diagnosis and Management of Disease

Prof Hanan Fouad , Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt

Personalized medicine exploits the benefits from the combination of integrated genomic information with the global monitoring of molecular components and physiological states. Personalized medicine involves Integrated Personal Omics Profiling (iPOP) analysis that integrate genomic, transcriptomic proteomic, metabolomic, autoantibodyomic information. These fields require in-depth knowledge in Bioinformatics.

Longitudinal personal omics profiling utilizes omics DATA for physiological and medical interpretation of healthy and disease states. Benefits of personalized medicine are: risk assessment, earlier diagnoses and earlier interventions. Molecular analysis could determine precisely which variant of a disease a person has, or whether an individual is susceptible to drug toxicities, to help guide treatment choices.

Molecular analysis could also help select patients for inclusion in, or exclusion from, late stage clinical trials. Better understanding of genetic variations could help scientists identify new disease subgroups or their associated molecular pathways, and design efficient drugs that target them.



Pag

e1

1

Keynote Lecture



Pag

e1

2

How to Link the Research Centers and Academic Units with the

Economic and industrial Sector? The ASTF Approach

Dr. Adbdalla Alnajjar, President, Arab Science & Technology Foundation (ASTF)

Arab Science & Technology Foundation (ASTF) aims to change Arabs' Culture though Science and Technology, and to develop and use their resources by transforming their knowledge into business.

Dr. Abdalla Alnajjar is an Arab Emirati Business magnate, Entrepreneur and Innovator. He is best known for his “Arab Science & Technology Foundation” (ASTF); the first state of the art Foundation in the Arab world which has under his leadership successfully identified and supported outstanding RDI and business activities throughout the Arab Region and beyond. His achievements created values and generated massive popularity for himself; he successfully, for three consecutive years, is named as one of the “Most Influential Arabs in the World”.

Dr. Abdalla, as an entrepreneurial minded person is always enthusiastic towards new challenges, developments, inventions and innovations. Apart from ASTF he, also, put foundation for companies specialized in developing new technologies and innovative products. Some of portfolio of his success establishments is Aashab Bio Industries, Arab Biotechnology Company, Rawafid for Project Managements, and AccuVis Bio. These companies has achieved substantial growth in past and is continuously expanding internationally.

Since the Youth are the backbones of every nation’s development, Dr. Abdalla continuous efforts for New Ideas and Innovations led to launch competitions like “Best Technological Business Plans” and “Investing in Technology Forum” that extracts, support & matches technpreneurs with investors.

His management & guidance’s was not limited for ASTF and his directly owned ventures, but it give unbound support, trainings and mentorship to Arab established companies. And he believes that the Region would not accelerate as fast as needed if not cooperated with the countries that have gone far with technological advancements. That’s why he invested time and resources in Vestec & Astroleap; few of successful companies ICT field worldwide.

Currently, Dr. Abdalla holds key positions in various companies and management advisory committees. He is a President of ASTF, a member of the Board of Directors for Vestec, Licensing Executives Society – Arab Countries (LES-AC), also in advisory board of Astroleap.



Pag

e1

3

ABSTRACTS



Pag

e1

4

Abstract 1:

Role of EPCs in Collaterals development for Patients with Chronic Total

Coronary Artery Occlusion and Neovascularization Development in an

Experimental Model with AMI

Mohamed T Abd El Aziz1, Emad A Abd El Nabi2, Magdi Abd El Hamid3, Dina Sabry1, Hazem M.

Atta4, Laila A Rashed1, Ashraf Shamaa5,Sohier Mahfouz6, Fatma M Taha1, Sherif Elrefaay7,

Doaa M Gharib1

1 Medical Biochemistry Department, Faculty of Medicine, Cairo University,

2Medical Biochemistry Department, Faculty of Medicine (NJ), King Abdulaziz University

3 Cardiology Department, Faculty of Medicine, Cairo University

4Clinical Biochemistry Department, Rabigh Medical College, King Abdulaziz University

5Surgery Department, Faculty of veterinary Medicine, Cairo University

6Pathology Department, Faculty of Medicine, Cairo University

7Nuclear Medicine Department, Faculty of Medicine, Cairo University

Abstract:

Purpose: We investigated the association between the number and function of endothelial

progenitor cells (EPCs) and the development of collateral formation in patients with single-

vessel coronary artery disease of chronic total occlusion (CTO). We aimed also to examine

the ability of EPCs to form new blood vessels and to differentiate into cardiomyoctes in an

experimental model (dogs) with acute myocardial infarctions (AMI).

Methods: Clinical part of our study included 20 patients who had CTO diagnosed by

coronary angiography. Patients were divided into 2 groups: group 1 (10 patients with good

collaterals) and group 2 (10 patients with poor collaterals) according to Rentrop

classification of coronary angiography. EPCs were isolated from peripheral blood samples

and cultured. Their numbers were counted as colony-forming units. Flow cytometry analysis

for CD34+ of cultured EPCs as an identification marker was performed. To assess the function

of EPCs, the expressed angiogenic genes [human vascular endothelial growth factor

receptor-2(hVEGFR-2) and endothelial nitric oxide synthase (eNOS)] from the culture cells

were measured by real-time qPCR and correlated with numbers of EPCs and collaterals

formation in CTO patients as assessed by coronary angiography. In the experimental part of

the study, EPCs were isolated from human cord blood and transplanted intramyocardially in

dogs with AMI. ECG was done , CK-MB and Troponin I were measured for the AMI animals to

assess improvement in cardiac ischemia. After scarification, histopathology was done to



Pag

e1

5

assess neovascularization and immunostaining was done to detect hEPCs trans-

differentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes

(hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted

EPCs.

Results: Group1 patients exhibited an increased number of EPCs (×106) than patients with

group 2 (2.39 ± 0.63 vs 1.37 ± 1.23, p = 0.033). Also, group 1 patients had higher expression

of hVEGFR-2 (5.63 ± 0.7 vs 4.33 ± 1.50 ) and eNOS ( 7.08 ± 0.69 vs 5.84 ± 1.63 ) genes in the

cultured cells compared with patients with group 2 (p= 0.024 & 0.041, respectively ). In AMI

dogs, histopathology showed increased neovascularization and immunostaining showed

presence of hEPCs newly differentiated into cardiomyocyte-like cells.

Conclusions: CTO patients with good collaterals have a significant increase in number and

function of circulating EPC. EPC-mediated angiogenesis might be associated with collateral

formation in patients with CTO. Human EPCs can mediate angiogenesis and they were able

to differentiate into cardiomyoctes in dogs with AMI.

Key words: Human EPCs, collaterals, coronary occlusion, VEGFR-2, eNOS, neovascularization,

canine, acute myocardial infarction.



Pag

e1

6

Abstract 2:

Activin A/Follistatin Mage-1 and 3 Transcripts: Markers for

Hepatocellular Carcinoma

Sabah Mahmoud 1, Hoda Elagaan2, Sherif Hegab3, Waheed El-Delgawi 1, Nevine El Deeb4

1Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Egypt.

2Department of Medicine (Hepatobiliary Unit), Faculty of Medicine, University of Alexandria, Egypt

3Radiology Department, Faculty of Medicine, University of Alexandria, Egypt

4Pathology Department, Faculty of Medicine, University of Alexandria, Egypt

Aims: To validate the role of serum activin A and follistatin as well as melanoma antigen

genes (MAGE-1 and MAGE-3) transcripts in the peripheral blood and malignant liver tissues;

as diagnostic markers for HCC

Methods: The study included; 15 HCC patients (group I), 15 liver cirrhotics patients (group II)

and 15 controls (group III).Serum free activin A and follistatin were estimated by ELISA. RNA

was extracted from peripheral blood of all subjects and from HCC tissues and surrounding

noncancerous liver tissues of HCC patients. The expression of MAGE-1 and MAGE-3 in these

samples was detected by RT-PCR.

Results: Serum activin A and follistatin levels were significantly elevated in group I and II as

compared to group III. They were also significantly elevated in group I as compared to group

II. No significant correlation was found between activin A and follistatin levels; or between

serum activin A and liver function parameters, tumor stage, or tumor characteristics. In

group I MAGE-1 gene was expressed in peripheral blood and HCC tissues in 53.3% (8/15

patients). MAGE-3 mRNA was detected in peripheral blood in 5 patients (33.3%) and in HCC

tissues in 6 patients (40.0%). None of these two genes was expressed in noncancerous liver

tissues or in peripheral blood of group II and III.

Conclusion: The deregulation in activin A/follistatin axis in liver malignancy suggests them to

be predictors for the occurrence of HCC, but as independent markers.

Key words: Atherosclerosis, PKG-Iα and PKG-Iβ



Pag

e1

7

Abstract 3:

Lipoprotein-associated Phospholipase A2 Levels as a Predictor of

Cardiovascular Risks in Patients with COPD and Obstructive Sleep

Apnea

Eman A E Badr1, Maha Yousif 2 and Suzan M Hazzaa

3

1Biochemistry, 2Chest diseases and 3Physiology Departments, Faculty of Medicine,

Menoufiya University, Egypt.

Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an inflammatory

mediator used as a novel marker for diagnosis of cardiovascular diseases and atherosclerosis

in human. Both chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea

(OSA) are associated with increased activation of inflammatory cells and molecular

mechanisms associated with atherosclerosis.

Aim: to study the relation between Lp-PLA2 level and the polysomnographic and spirometric

data in patients with OSA, COPD and overlap syndrome (COPD+OSA).

Methods: Sixty patients were recruited, divided into 4 groups (15 in each group) based on

their polysomnographic and spirometric data ; group I (normal), group II (OSA), group III

(COPD) and group IV (overlap syndrome). Fasting serum samples were used to estimate lipid

profile and Lp-PLA2 concentrations.

Results: apnea-hypopnea, arousal, desaturation indices, lipid profile and Lp-PLA2 levels were

significantly increased in all patients groups compared to control. The level of Lp-PLA2 was

significantly increased in overlap syndrome than OSA and COPD patients and was positively

correlated with the severity of arousal in all patients groups.

Conclusion: patients with overlap syndrome are more liable to cardiovascular disorders than

either OSA or COPD alone and Lp-PLA2 may be used as an independent predictor of

cardiovascular disorders in patients with OSA, COPD and overlap syndrome.

Key words: Lipoprotein-associated phospholipase A2, obstructive sleep apnea (OSA), chronic

obstructive pulmonary diseases (COPD), overlap syndrome, arousal.



Pag

e1

8

Abstract 4:

The Association between Cyclooxygenase 2 Gene (rs20417)

Polymorphism and Coronary Artery Disease at Suez Canal University

Hospital in Ismalia

Emad Ismail 1 , Amed Wl Hawary2, Amany Yousef El kazaz1, Noha Abdel Fadeel 1

1Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismalia,

Egypt

2Cardiology Department, Faculty of Medicine, Suez canal University

Coronary artery disease (CAD) is one of the major contributors to early mortality.

Atherosclerosis is an inflammatory process mediated by prostaglandins and several

interleukins. Alterations in prostaglandin expression or activity can significantly modify CAD

risks. We evaluated the association between single nucleotide polymorphism (-765G > C)

(rs20417) in Cyclooxygenase 2 (Cox-2) gene and coronary artery disease and its relation to

serum IL-6 level in 61 angiographically proven coronary artery disease patients attending to

cardiac care unit and outpatient clinic of Suez Canal University Hospital and 25 sex and age -

matched healthy controls.

The Cox-2 (-765G > C) gene polymorphism was evaluated by PCR/RFLP method. IL-6 levels

were determined by Immulite.

Genotype GC was more frequent in patients (73.8%) (odd ratio: 5; 95% CI: 1.85-13.54; P=

0.001) while GG genotype was more frequent in controls (60%) (P=0.00). Allele C was found

to be associated with CAD (odd ratio: 4.043; 95% CI: 1.895-8.626; P= 0.0003).

The highest serum IL-6 value was observed in patients with CAD especially in multiple

vessels disease compared with controls. Carriers of C allele (GC, CC) had higher levels of IL-6

and had more stenosis in multiple coronary vessels. In conclusion, Cox-2 (-765G > C) gene

polymorphism may be considered as a genetic risk factor for CAD in Suez Canal area. Carriers

of the at-risk C allele had higher IL-6 levels causing an increased risk to develop CAD.

Key words: CAD; Cox-2 (-765G>C); polymorphism.



Pag

e1

9

Abstract 5:

Apo CIII 3238C/G Gene Polymorphism Influences Oxidized Low-density

Lipoprotein with a Risk of Essential Hypertension among Egyptians

Maivel Heshmat Ghattas1, Heba K Badawy2, Noha Mesbah3 , Dina Abo ElMatty3

1Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia , Egypt

2Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical industries, Sinai University, North Sinai, Egypt.

3 Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

Introduction: Essential hypertension is thought to be a polygenic and multifactorial disease. Apo CIII gene has been identified as a candidate region for hyperlipidemia and atherosclerosis. The current study tested the hypothesis that Apo CIII 3238 C/G gene polymorphism may affects oxLDL level and increasing the susceptibility to essential hypertension Methods: A total of 120 hypertensive patients and 130 aged and sex matched controls were selected from North Sinai area. All subjects are Egyptians and had the same ethnicity. Genotyping of the ApoCIII 3238C/G was performed by polymerase chain reaction restriction fragment length polymorphism. Lipid profile and serum oxidized low density lipoprotein (oxLDL) were determined. Results: The minor G allele of Apo C III 3238C/G gene polymorphism was more frequent in hypertensive patients than the controls (OR= 2.09, 95% CI= 1.16-3.78, p= 0.013). The CG genotype carriers were associated with higher levels of triglycerides and oxLDL in patients and control subjects. No significant difference was observed in other lipid parameters. However, the HDL levels were higher in CG genotype carriers compared to carriers of CC genotype. Conclusions: The results of the present study show that the Apo CIII 3238C/G SNP is associated with susceptibility to essential hypertension and provide the first evidence that apoCIII 3238 C/G affecting serum oxLDL. Therefore, this study bridges genetic susceptibility to essential hypertension with atherogenecity and the potential long-term risk of cardiovascular diseases in North Sinai population.



Pag

e2

0

Abstract 6:

Lack of Association between the -2548G/A Polymorphism of the Leptin

Gene and Hypertension in Saudi Adult Patients

Amany Osama Mohammed1 , Tahia Saleem 1, Lotfi Bindahman 2

1Medical Biochemistry, Assiut University, Egypt

2Biomarkers Research Program, Biochemistry, King Saud University, Saudi Arabia

Background: Leptin is classified as adipokine, plays an important role in the regulation of

blood pressure. A common polymorphism in the promoter of the human leptin gene (G-

2548A) may play a role in the pathogenesis of hypertension.

Objective: to investigate the association between the leptin gene polymorphism (-2548G/A)

and hypertension among Saudi adult obese patients.

Patients and Methods: 206 subjects among 50 healthy controls, 80 normotensive obese and

76 hypertensive obese patients, their aged 40-60 years. Analyses of -2548G/A polymorphism

of the leptin gene were made by PCR-RFLP. The genotypes (GG, GA, and AA) and alleles (G

and A) were scored and the frequencies were estimated. The frequencies of alleles and

genotypes in three groups were compared. Serum fasting blood glucose, lipids, leptin and

insulin were determined.

Results: the study showed that the AA genotype of -2548G/A variant of the leptin gene was

significantly more common in subjects with higher fasting glucose levels (P=0.02) while GA

genotype was more common in individuals with hyperleptinemia (p=0.04). On the other

hand no association was elicited with either systolic or diastolic blood pressure.

Conclusion: The study showed that -2548G/A of leptin gene polymorphism was not

associated with obesity or hypertension, whereas the study showed that the genotypes

distribution of -2548G/A variant of the leptin gene are associated with plasma leptin, and

glucose levels in set of Saudi individuals. Moreover, the genotypes AA and GA as well as A

allele might be an important risk factor predisposing healthy subjects to T2DM.



Pag

e2

1

Abstract 7:

Comparison between Serum Levels of Retinol Binding Protein-4 and Total Thiols in Generalized Obesity and Abdominal Obesity Regarding Cardiovascular Risks.

Naglaa Idriss 1 , Ahmed Ahmed2, Salwa Dimitry2, Eman Abdel Aal2

1 Medical Biochemistry Department, Faculty of Medicine, Assuit University

2 Cardiology Department, Faculty of Medicine, Assuit University

Background: Retinol-binding protein-4 (RBP-4) is an adipocyte-secreted hormone considered

to link obesity with cardiovascular complications. Given that the oxidative stress, which

distinguishes by the overproduction of reactive oxygen species (ROS) has been concerned in

the pathophysiology of obesity. We evaluated serum levels of RBP-4 and Total thiols (TT) in

generalized obesity (GO) and abdominal obesity (AO) on the topic of cardiovascular risk

factors. As well as to study other risk factors such as venous levels of Glycated haemoglobin

(HbA1c%), C- reactive protein (CRP) and lipid profile.

Method: Sixty obese patients were recruited [30 abdominally obese (AO) patients [(15 male;

mean (SD); 49.5(5.5) years) were measured by Waist Circumference (WC > 102 cm for men

or > 88 cm for women and the WC divided by that of the hips of >0.9 for men and >0.85 for

women)] And [30 generalized obese (GO) patients (22 male; mean(SD); 42.5(8) years)

measured by Body mass index (BMI). *BMI ≥ 30-34.9 kg/m2, with normal WC]; Compared to

20 healthy controls (HC) (14 male; mean (SD) 36.5(6) years), serum levels of RBP-4 were

measured by (ELISA), serum levels of (TT) were measured by colorimetric methods, plasma

HbA1c%, lipid profiles and CRP were also determined.

Results: AO had significantly higher circulating RBP-4 levels in compared to GO and HC

(p<0.0001). Total thiols levels had significantly lower in AO patients compared to GO and HC

(p<0.0001). CRP significantly elevated in AO compared to other groups (p<0.003). No

Significant difference between serum levels of TT in GO compared to HC (p=NS). Total serum

cholesterol, triglycerides and HB1Ac% increased with BMI, WC and waist hip ratio (WHR).

Conclusion: The study reveals that RBP-4 is autonomously related to visceral fat

accumulations and might contribute to the pathogenesis of visceral obesity with

cardiovascular diseases. These results described here in assist to clarify the beneficial effect

of TT against these conditions and define the potential clinical applicability of oxidative

stress in cardiovascular diseases.

Key words: Retinol binding protein4-abdominal obesity-generalized obesity-cardiovascular

disease



Pag

e2

2

Abstract 8:

A Curcumin Derivative on Notch1 Signaling in Hepatocellular

Carcinoma

Walaa Aly Ibrahim, Nagwa Rhoushdy, Amira Hassouna Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University

Backgroud: The Notch1 receptor and signaling pathway is up regulated in HCC. This

increases the oncogenic potential of the cell through prevention of differentiation and

inhibition of apoptosis. The present study was conducted to evaluate the effect of

mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on hepatocellular

carcinoma (HCC) both in vivo in hepatoma induced animal model in rats and in vitro on

HepG2 cells (hepatoma cell line), and to investigate their effect on Notch1 signaling pathway

target genes.

Methods: This work included in vivo study and in vitro study. The in vivo part: One hundred

rats were divided equally into: control group, control group received MSCs, control group

received a NCD, HCC group induced by diethylnitroseamine (DENA) and CCl4, HCC group

received MSCs only, HCC group received a NCD only, HCC group received MSCs and a NCD

simultaneously, HCC group received MSCs followed by a NCD 2 weeks later, HCC group

received MSCs that was pretreated with a NCD in vitro and HCC group received MSCs

conditioned medium (CM).The in vitro part: HepG2 cells were divided into:HepG2 control

group, HepG2 cells treated with MSCs CM, HepG2 cells treated with a NCD, HepG2 cells

treated with MSCs CM and a NCD and HepG2 cells treated with MSCs CM (CM of MSCs

pretreated with a NCD). Histopathological examination and gene expression of Notch1

signaling target genes by real time, reverse transcription-polymerase chain reaction (RT-PCR)

in rat liver tissue and HepG2 cells, serum levels of alpha fetoprotein, ALT and albumin were

assessed. In addition, HepG2 proliferation assay was performed in all group.

Results: Histopathological examination of liver tissue from animals which received DENA-

CCl4 only, revealed the presence of pleomorphic cancer cells. Administration of MSCs or a

NCD into rats after induction of experimental HCC improved the histopathological picture

with residual pathology while administration of MSCs and a NCD or MSCs that was

pretreated with a NCD in vitro showed restoration of liver parenchyma. Gene expression in

rat liver tissue and HepG2 cells demonstrated that Notch1 and its target genes (Hes1 and

cyclinD1) were downregulated in all treated groups with more suppressive effect in the

groups treated with both MSCs and a NCD. Serum levels of AFP, ALT were significantly

decreased in all treated groups, serum levels of albumin was significantly increased in all

treated groups. Also, treated HepG2 cells showed significant decrease in proliferation rate.



Pag

e2

3

Conclusion

Administration of MSCs, a NCD, MSCs and a NCD, MSCs pretreated with a NCD and MSCs CM

in chemically induced HCC down regulated Notch1 and its target genes (Hes1 and Cyclin D 1)

and improved the histopathological picture. This effect was more profound in the HCC

groups that received MSCs and a NCD and those that received MSCs pretreated with a NCD.

These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can

be considered as a therapeutic target in HCC.

Key words: Notch signaling, hepatocellular carcinoma, MSCs, NCD



Pag

e2

4

Abstract 9:

Single Nucleotide Polymorphism of IL-10 and IL- 28B as predictors to the response of interferon therapy in HCV infected children

Zeinab A.Nour1, Olfat G. Shaker1, Yasser H. Nassar1, MonaEl-Razki2

1Departments of Medical Biochemistry and Molecular Biology, 2Pediatrics Department, Faculty of Medicine, Cairo University

Background & Aims: We aim to detect the relation between SNPs of IL-10(–1082, –819, and

–592) and IL28B gene (rs12979860) and prediction of response to Pegylated interferon

(PegIFN) plus Ribavarin(RBV)in Egyptian pediatric subjects with genotype 4.

Methods: ARFLP-PCR and Real time PCR techniques were used to genotype 34 pediatric

patients with HCV for IL-10 SNPs and IL-28B SNP respectively. Patients received (PegIFN) and

(RBV) for 48 weeks subdivided according to their response to treatment into responders (20)

and non-responders (14), and 20 healthy subjects.

Results:A significant difference (p<0.005) was observed in IL-28B rs12979860 genotype

frequencies between responders and non-responders. In responders CC genotype had

greater frequency than CT and TT genotypes (60%, 30%, 10%) respectively with C allele in its

wild genotype more likely to respond to treatment than in its mutant types.IL-10 at -819

showed significant difference in its genotype frequencies between responders and non-

responders, TT genotype had greater frequency in responders than CT and CC (55%, 20%,

25%) respectively. Subjects with T allele (CT/TT) showed higher rates of response than those

with no T allele (CC), its protective effect in both recessive and dominant forms.

Conclusion: Il-28B CC genotype as well as the IL-10 (-819) TT genotype are significantly

associated with response to PegIFN and RBV for pediatric patients with HCV infection

genotype 4. These SNPs can be used for predicting response to treatment before patient is

prescribed to the expensive PegIFN-RBV therapy.

This work was supported by Cairo University.



Pag

e2

5

POSTERS



Pag

e2

6

Poster 1:

Breast Cancer Proliferative Activity: Is It the Source of Free Serum DNA?

Taha Hewala 1 , Nadia Abd El-Moneim2

1Department of Radiation Sciences, Medical Research Institute, Alexandria University, Egypt.

2Department of Cancer Management and Research , Medical Research Institute, Alexandria University, Egypt.

Aim: To study the relationship between serum DNA and breast cancer proliferative activity,

then compare the diagnostic and prognostic value of serum DNA, TPS and CEA.

Subjects and Methods: A total of 100 breast cancer patients before surgery and 50 normal

healthy controls were analyzed for serum DNA, TPS and CEA.

Results: Serum DNA levels were nonsignificantly correlated with serum TPS levels. Serum

DNA, TPS and CEA levels were significantly higher in patients than controls. Using ROC curve

analysis, serum TPS and DNA had the highest AUC compared to serum CEA. Neither serum

TPS, DNA nor CEA correlated with breast cancer clinicopathological data. Only serum CEA

was significantly correlated with patients' disease-free survival, but serum DNA and TPS did

not show this correlation.

Conclusion: Neither Breast cancer mass nor proliferative activity is the source of free serum

DNA. Serum TPS is superior to DNA and CEA as a diagnostic marker for breast cancer. Only

preoperative serum CEA has a prognostic role in predicting relapse of breast cancer patients,

but not serum TPS and DNA.



Pag

e2

7

Poster 2:

Evaluation of ATP7B Gene Mutation as a Useful Tool in Genetic Counseling for Wilson Disease in High Risk Egyptian Children

Ayman Azzam 1 , Manar Obada 1, Elsayed Salama3, Eman Badr2, Tarek Abdel Hakim 2

1Department of Clinical Biochemistry, National Liver Institute, Menoufiya University

2Department of Medical Biochemistry, Faculty of Medicine, Menoufiya University

3Department of Pediatric Hepatology, National Liver Institute, Menoufiya University, Egypt

Background: Wilson’s disease (WD) is an autosomal recessive disorder of copper

metabolism. It is caused by different mutations in ATP7B gene.

The aim of the present study is to evaluate His1069Gln mutation in exon 14 of ATP7B gene

as a useful genetic counseling tool in Egyptian children with Wilson's disease and their

siblings.

Subjects and Methods: the present study was conducted on 94 subjects classified into three

groups: group (I) included 20 index cases with WD. Group (II) included 27 individuals who are

siblings of all patients diagnosed with Wilson disease. Group (III) included 47 apparently

healthy subjects as a control. All studied subjects were subjected to full history taking,

general and clinical examination, laboratory investigations including liver function tests,

serum levels of ceruloplasmin and copper as well as 24 urinary copper. His1069Gln mutation

of ATP7B gene was identified using a semi-nested polymerase chain reaction (PCR) followed

by restricted fragment length polymorphism assay (RFLP)

Results: this study showed that 80% of Egyptian children with WD presented with hepatic

manifestations. There was significant increase in the frequency for His1069Gln mutation

25% (5 cases) of WD patients and 14.8% (4 cases) among siblings. His1069Gln mutation was

not detected in all 47 healthy controls. Mutation status did not correlate with liver function

tests, serum ceruloplasmin nor serum copper (p>0.05). There was no significant correlation

between His1069Gln polymorphic state and WD phenotype and age at diagnosis (p>0.05).

Conclusion: His1069Gln ATP7B gene mutation might be used as a useful biomarker for

diagnosing WD in high risk children. Detection of this mutation could be included with high

priority in the molecular testing screening panel for Egyptian WD patients and their

asymptomatic first degree relatives to know the carrier rate in our community.



Pag

e2

8

Poster 3:

Study of Serum Micro-RNA-221 Expression in Egyptian Hepatocellular Carcinoma Patients

Samir Abdelmoneim 1, Moshira Mahmoud 1, Gehan H Ibrahim1, Loaa A Tageldeen1, Seham

Omar 2, Nagwa Aly 1

1Department of Medical biochemistry, Suez Cana University, Ismailia, Egypt

2Department of internal Medicine, Faculty of Medicine Suez Canal University, Ismailia, Egypt

Hepatitis C virus (HCV) is considered the most common etiology of chronic liver diseases in

Egypt including hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) are small

endogenously expressed non-coding RNAs that negatively regulate expression of protein-

coding genes. Recently, circulating miRNAs have been reported as promising biomarkers for

various pathologic conditions. MicroRNA-221 (miR-221) was shown to be upregulated in HCC

tissues however, circulating level of miR-221 in chronic hepatitis C (CHC) or CHC-related HCC

were not consistently investigated.

This study aimed to estimate serum level of miR-221 in CHC patients with and without HCC

and to evaluate its role as a biomarker in HCV related liver pathologies.

RNA was extracted from the sera of patients with CHC, CHC-related HCC and from healthy

controls. MiR-221 was quantified by reverse transcription and real-time PCR (RT-PCR).

Serum miR-221 was slightly higher in HCC patients than in healthy controls, but this did not

reach statistical significance (P= 0.39).

Evaluation of the relation between miR-221 expression and the clinicopathological features

of the tumors revealed no significant correlation. However, serum miR-221 was significantly

elevated in CHC patients compared to healthy controls (P= 0.003) and HCC patients (P=

0.006). Moreover, miR-221 and ALT levels were positively correlated (r= 0.68, P= 0.03) in

CHC patients. Receiver operating characteristic (ROC) curve clearly discriminates between

CHC patients and healthy controls, (area under ROC curve = 0.96, 95% CI [confidence

interval], 0.865-1.06, P= 0.005), with 90% sensitivity and 80% specificity at a cut off value of

3.04 fold increase.

Conclusion: Our results suggest that serum miR-221 might potentially serve as a novel

biomarker for liver injury caused by HCV but not for HCV- related HCC. Key Words: Hepatitis

C, hepatocellular carcinoma, microRNA, miR-221



Pag

e2

9

Poster 4:

Urinary Cytokeratin (20) mRNA as a Diagnostic Tool for Bladder Cancer

Tarek F Abd El-Akim1, Maather K El-Shafaee1, Manal A Safan1, Ashraf A Dawood1, Sally M El-Hefnawy1 and Abd El-Aleem M El-Deraee2

1 Departments of Medical Biochemistry, Faculty of Medicine, Menoufiya

2 Department of Urology, Faculty of Medicine, Menoufiya University

Transitional cell carcinoma of bladder (TCCB) is the most common malignancy of the genitourinary system with high recurrence rate.

Aim of the study: The current study was conducted to evaluate cytokeratin (20) mRNA expression in urine as tumor marker in comparison with urine cytology for detection of bladder carcinoma.

Subjects and Methods: This study was carried out on 50 subjects divided into three groups, group I: included 25 patients with transitional cell carcinoma of bladder (TCCB), group II: included 15 patients with benign urological disorders and group III: included 10 healthy volunteers. All subjects submitted to full history taking, clinical and radiological investigations and real-time polymerase chain reaction (RT-PCR) for urinary cytokeratin (20) mRNA expression and analyzed according to the following parameters: urinary cytology, tumor stage and grade.

Results: Urinary cytokeratin 20 mRNA expression was significantly higher in TCCB group than in benign urological disorders group and control group. Urinary cytokeratin 20 mRNA expressions is significantly increased with higher tumor stage and in invasive tumors more than superficial tumors but it doesn't differ as regarding tumour grade. There is significant positive correlation between urinary CK 20 mRNA and tumor stage but not with tumor grade. At a cutoff point of ≥ 1.4969, the sensitivity of urinary CK 20 mRNA in diagnosis of bladder cancer is 96%, the specificity is 93%, and accuracy of the test is 95 %. The combined use of urinary CK 20 mRNA and urinary cytology, have improved the sensitivity and the accuracy more than the use of a single marker.

Conclusions: Detection of urinary CK20 mRNA expression in exfoliated urothelial cells using real time PCR technique is a powerful noninvasive, sensitive approach for early detection of bladder cancer and can complement cytology and histopathology in the diagnosis of bladder cancer. Urinary CK20 mRNA expression had superior sensitivity over urine cytology.



Pag

e3

0

Poster 5:

Utility of Serum and Urinary Lipocalin-2 in Predicting Renal Impairment in Patients with Chronic Ischaemic Heart Failure

Manal Ellaithy1, Maather K El-Shafaee1, Walaa Abdel-Aziz2, El Sayed Abou Elnour1, Ibrahim Elmadbouh1

1 Department of Medical Biochemistry, Faculty of Medicine, Menoufiya University

2Cardiology Department - Faculty of Medicine - Menoufiya University

Chronic Heart failure and chronic kidney disease share a number of risk factors. Lipocalin–2 is a 21 kDa glycoprotein first isolated by Kjeldsen and colleagues in 1993.

The aim of the study is to assess whether lipocalin-2 could represent a novel, sensitive marker of kidney function in patients with ischemic chronic heart failure.

Subjects and Methods: This study was carried out on 45 subjects: 35 patients from Cardiology Department and Intensive Care Unit, Menoufiya University Hospital and Shebin-Elkom Educational Hospital and 10 healthy subjects served as controls. Subjects were divided in to 2 groups: Group I: Included 35 patients with chronic ischemic heart failure (IHF) (24 males, 11 females). Their ages ranged between 50-70 years. Coronary angiography was performed for all these patients. Group II: Included 10 age and gender matched healthy subjects served as controls (9 males, 1 female). Their ages ranged between 55-68 years. All studied subjects were submitted to the following: full history taking, thorough clinical examination, laboratory investigations including, determination of serum urea, Serum creatinine, creatinine clearance, body weight in kilograms, lipid profile including total cholesterol, serum triglycerides, Serum HDL-c and Calculation of low density lipoprotein cholesterol (LDL-c), serum albumin, serum and urinary lipocalin-2 were determined.

The results: Serum urea and creatinine were significantly higher in patients with chronic IHF when compared to controls, while creatinine clearance and serum albumin were significantly lower in patients when compared to controls. Serum and urinary lipocalin-2 were significantly higher in chronic IHF patients when compared to controls. There was significant positive correlation of serum lipocalin-2 with serum creatinine, while there was negative correlation with serum albumin and creatinine clearance. There was significant positive correlation of urinary lipocalin-2 with serum creatinine, while there was significant negative correlation with creatinine clearance and serum albumin. At a cut off value of 130.5 ng/ml, serum lipocalin-2 can differentiate between patients with renal impairment and controls with sensitivity of 89% and specificity of 100%. At a cut off value of 10.8 ng/ml, urinary lipocalin-2 can differentiate between patients with renal impairment and controls with sensitivity of 97% and specificity of 90%.

Conclusion: Serum and urinary lipocalin-2 represent novel and sensitive markers in detection of renal impairment in patients with chronic ischemic heart.



Pag

e3

1

Poster 6:

Evaluation of Serum Omentin-1 and Visfatin Levels in Women with Polycystic Ovary Syndrome

Manal A. Safan1, Ashraf A. Ghanem2


2Obstetrics and Gynecology Department, Faculty of Medicine, Mansoura University

Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrine disorders in women of reproductive age. Adipose tissue seems to have an important role in the development and maintenance of the condition.

Aim of the study: The current study was conducted to evaluate serum levels of omentin-1 and visfatin and omentin-1/visfatin ratio in PCOS patients compared with healthy normal control subjects.

Subjects and Methods: Data from twenty five women with PCOS (ages 22–36 years) were compared with twenty healthy control women with regular menstrual cycles (ages 23–35 years). In each subject biochemical, hormonal, and ultrasonographic parameters were studied.

Results: The study showed that serum omentin-1 concentrations were significantly decreased while serum visfatin concentrations were significantly increased in the PCOS patient group compared to the control group. There was a significant negative correlation between the serum levels of omentin-1 and visfatin both in the control and in the PCOS patient group. Also there was a significant negative correlation between omentin-1: visfatin ratio and insulin in PCOS patient group, however there was no significant correlation in the control group. In PCOS patients there were significantly increased body mass index (BMI), waist-to-hip ratio (WHR), serum fasting insulin levels and homeostasis model assessment-insulin resistance (HOMA-IR). The results also showed that 90.33%, 92.0% and 95.67% of PCOS patients had abnormal omentin-1: insulin ratio, Omentin-1: HOMA-IR ratio and omentin-1: visfatin ratio respectively according to the cutoff values ≤ 25.98, ≤ 103.43 and ≤ 14.72 respectively.

Conclusions: The omentin-1 ratios, the percentage of their sensitivity and also the difference of their ranges in PCOS patients and control group may give a different perspective in understanding the etiology of PCOS and can be used for the diagnosis of PCOS. The study also showed that omentin-1/visfatin ratio may play a paracrine or endocrine role in modulating insulin sensitivity. Running title: Omentin-1, Visfatin, and PCOS Key words: Omentin-1, Visfatin, PCOS and ELISA



Pag

e3

2

Poster 7:

The Value of Urinary Survivin as a Marker in Bladder Cancer

Tarek Fouad 1, Maathir Kamel 1 , Rania Azmy1, Mohamed Nor el din2, Asmaa Abdo3


2Department of Urology, Faculty of Medicine, Menoufiya University

3Department of Pathology, Faculty of Medicine, Menoufiya University

Bladder cancer constitutes 30% of all cancer cases admitted to Egyptian National Cancer Institute. There are commercially available and promising investigational urine-based bladder cancer marker systems. Among of these promising investigational urine-based bladder cancer marker is survivin.

The aim of this work is to study the value of urinary survivin as a marker in diagnosis of bladder cancer.

This study was carried out on 80 subjects:. They divided into 3 groups: groupI, included 40 patients with bladder cancer, this group is subdivided into 2 subgroups according to tumor stage into: subgroup I A (advanced ): it included 25 patients and subgroup IB (superficial): it included 15 patients, groupII, included patients with benign urological disorders, groupIII, included 20 age and gender matched healthy controls. All subjects were submitted to full history taking, general and local urological examination, urine analysis, abdomino pelvic ultrasonography and CT, measurement of urinary survivin , serum antibilharzial antibodies, Urine cytology and cystoscopic biopsy and histopathological examination in cancer patients group and benign group.

The results showed that, there is highly significant statistical difference among studied groups as regards cytology and survivin, while there is a significant statistical difference as regards antibilharzial antibodies. Cytology has lower sensitivity, accuracy and negative predictive value than survivin, while it has higher specificity and positive predictive value than survivin, on the other hand, the sensitivity of survivin and cytology is (100%), the specificity is (95 %) and the accuracy is (97 %). It has high positive predictive value (95%) and high negative predictive value (100%). Positive cytology cases and survivin are significantly higher in advanced group when compared to superficial group. Also number of superficial cases which can be diagnosed by + ve survivin is significantly higher than those diagnosed by +ve urine cytology, the prevalence of high grade is significantly higher than low grade among + ve survivin and urine cytology. Also number of low grade cases which can be diagnosed by + ve survivin is significantly higher than those diagnosed by +ve urine cytology.

Conclusion: Our results suggested that urinary survivin test appears to be reliable non invasive diagnostic test to identify patients with bladder cancer. The sensitivity of survivin test was superior to that of urine cytology in diagnosis of bladder cancer and combination of both survivin and cytology give better sensitivity, survivin is also better for diagnosis of both superficial and advanced, low and high grade bladder cancer, so survivin is helpful in preventing unnecessary cystoscopy.



Pag

e3

3

Poster 8:

Serum HSP27, Traditional Risk Factors and Diabetic Nephropathy in Type 2 Diabetic Egyptians

Shahenda Mahgoub 1, Mahmoud Youns 1 , Atef Bassyouni2, Zeinab Hassan 1

1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo, Egyp

2National Institute of Diabetes and Endocrinology, Kaser Alainy Cairo, Egypt

Heat shock protein 27 (HSP27) has been involved in different kidney diseases playing both protective and counter-protective roles. Stressful conditions that include oxidative stress as diabetes and chronic kidney disease promote cells to over express HSP27. Here, serum Hsp27 levels in 72 Egyptian type 2 diabetic subjects with and without diabetic nephropathy (DN) were investigated.

Our study included three diabetic groups: 14 diabetic control subjects (DC), diabetic risk group (DR) which included 28 diabetic subjects with one or more of the traditional risk factors for diabetic nephropathy: Hypertension and/or dyslipidemia, and 30 diabetic nephropathy (DN) patients with different DN stages (DN1= 6, DN2= 9 and DN3= 15 patients) according to the decrease in the estimated glomerular filtration rate.

Serum Hsp27 concentrations were significantly higher in patients at risk to as well as, with evident DN compared to DC subjects (p<0.01). Moreover, before microalbuminuria becomes evident, serum Hsp27 levels showed increased area under the curve with increasing risk factors for DN in addition to higher sensitivity and area under the curve compared to common traditional markers for DN diagnosis as creatinine and microalbuminuria.

In conclusion, our results showed that serum HSP27 may be used as an early marker for diagnosis of DN as a microvascular complication in patients with type 2 diabetes mellitus since serum Hsp27 levels appear to be associated with the risk for DN as well as with manifest DN.



Pag

e3

4

Poster 9:

Clinical Value of Heat Shock Protein 60 in Cardiovascular Complications in Egyptian Patients with Type 2 Diabetes

Heba Sharaf ElDin 1 , Sahar Ali 1, Ashraf Amin2, Zeinab Hassan1

1Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University

Helwan , Egypt

2 National Diabetes Institute, GOTHI, Cairo, Egypt

Hyperglycaemia and hyperinsulinaemia, hallmarks of the postprandial state, have been also

associated with increased oxidative stress contributing to vascular injury. The development

of cardiovascular disease (CVD) is a main complication of diabetes mellitus (DM). Hsp60 is

over-expressed after cells exposure to stressful conditions that include oxidative stress like

diabetes as well as CVD. The association between serum Hsp60 levels and development of

diabetic complications is unknown. Although heat shock protein 60 (Hsp60) is implicated in

the pathogenesis of atherosclerosis, its role in cardiovascular disease is uncertain

Aim of the work: evaluate the potential value of serum Hsp60 level in the development of

cardiovascular complications in Egyptian patients with type 2 DM.

Patients and Methods: It was determined using The AssayMax Human Hsp60 ELISA kit in 18

diabetic control subjects and 62 diabetic patients with different risks to (CVD). All groups

were age and sex matched.

Results: Serum Hsp60 concentrations were significantly higher in diabetic patients with

diferent risks to CVD compared to diabetic control subjects (p<0.05). Moreover, its level

showed higher sensitivity and area under the curve compared to common traditional

markers (ratio 1 & ratio 2) of CVD.

Conclusion: our results showed that presence of Hsp60 in diabetic patients was associated

with the development of CVD complications. Hsp60 showed superiority in sensitivity

compared to other traditional biochemical parameters so could serve as an early marker for

diagnosis of diabetic CVD.



Pag

e3

5

Poster 10:

DNA-Based Vaccination Using Multiple Schistosoma Mansoni Genes

Mohamed Saber1, Hanan Omar1, Mohamed Shemis 1, Tarek Abou Shousha2 , Mahmoud

Romeih1

1Department of Biochemistry, Theodore Bilharz Research Institute, Imbaba, Giza, Egypt

2Department of Pathology, Theodore Bilharz Research Institute, Imbaba, Giza, Egypt

Evaluation of immunogenicity and protective efficacy of immunization with DNA-based

vaccination using multiple Schistosoma mansoni genes has been designated in this study.

Female Swiss albino mice were immunized with DNA vaccine cocktail containing plasmids

coding the full length of SMALDO, Smfim and SM21.7 genes. DNA-Cocktail vaccinated mice

were then infected with S. mansoni and evaluated for infection/inflammation markers and

compared to non-vaccinated or empty plasmid vaccinated mice controls.

The DNA cocktail vaccination conferred 43.6% reduction in worm burden (p<0.001), and

achieved 49.6% and 58.2% reduction in the number of eggs/g hepatic and intestinal tissue

respectively (p<0.001). Intramuscular injection of the DNA vaccine cocktail, successfully

induced IgG antibody response. Hepatic tissue expression of cytokines, measured by mRNA

levels, showed increased IFN-ɣ, IL-2 and IL-12 in the DNA cocktail vaccinated mice group,

while IL-4 and IL-10 increased in the control group. Significant reduction in granuloma

diameter (33.1%) and count (46.3%) was recorded in the vaccinated group (p<0.05). The

percentage of degenerated ova was increased significantly in the vaccinated group

compared to the control (p<0.001).

The DNA vaccine cocktail used in this study induced a significant level of protection against

S. mansoni challenge infection; it stimulates specific humoral and cellular immunity and has

antipathological effect.



Pag

e3

6

Poster 11:

Endosomal Galectin-3 Modulates Repair of Hepatic Cell Injury: Possible

Roles of TGF-β

Mohamed A. Abd EL-Aziz

Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University (Assiut)

Galectin-3 (Gal-3) plays an important role in the phagocytosis and angiogenesis of damaged

hepatic cells induced by CCl4.

We investigated Gal-3 found in the endosomal fraction of rat hepatic cells produced as a

repair protein in three groups of animals; 48, 72 and 96 hours after administration of a single

dose of CCl4.

We studied the following proteins using western blot analysis; signaling proteins related to

repair of this hepatic damage, endosomal marker to specify the endosomal fraction under

study and finally proteins may be mediated in the mechanism of repair of this hepatic

damage. We found overexpression of both rab-5 and rab-7 proteins as endosomal markers

for the endosomal subcellular fraction in the liver homogenate for the three groups of

animals and their control, also Gal-3 which is widely spread among different types of cells

and subcellular fragments including endosomes was highly expressed in the endosomal

fragments of the different animal groups.

Endosomal Gal-3 has numerous biological effects and seems to be involved in different

physiological and pathophysiological conditions such as endothelial angiogenesis and

phagocytosing the damaged apoptotic and necrotic cells from the damaged hepatic cells,

both effects were mediated by transforming growth factor beta (TGF-β) as explained by the

expression of TGF-β in all studied animal groups that was parallel to the expression of Gal-3

in all studied groups



Pag

e3

7

Poster 12:

Study the Association of the PON1 Q192 R Gene Polymorphism in

Acute Myocardial Infarction Patients in Ismalia City

Nagwan Abd el Azyz Sabek , Fathalla Hasan, Salwa S Fiesl, Dahlia I Badran, Gamela Naser

Faculty of Medicine, Suez Canal University, Ismailia

Paraoxonase (PON1) is a 44 kDa Ca+2 dependent glycoprotein and is located on the surface

of high density lipoprotein (HDL). PON(s) acts as important guardian against cellular damage

from toxic agents and oxidized lipids in the plasma low-density lipoproteins. The ability of

PON to limit oxidation represents a major antiatherogenic mechanism; these data have

demonstrated that the PON content of HDL is a major determinant of the antioxidant

function and consequently, the anti-inflammatory capacity of the lipoprotein.

In the present study the association of gene variations in PON1 (rs 662) and MI among

Egyptian patients living in Ismailia city has been examined. PON1 (rs 662) gene

polymorphism was studied in 50 acute MI patients and 30 controls subjects using Real-Time

polymerase chain reaction. The prevalence of PON1 alleles was not significantly different in

MI patients compared to control subjects (P> 0.05). Although the higher frequency of the

major or protective allele (C) in the control group (41.7%) more than in the MI group (31%),

which didn’t reach a significant level (OR=0.63, 95% CI. 82 to 3.1, P=1.7).

Our results shows that risk factors of MI being significantly higher in patients than control

group; hypertension (P<0.0001), diabetes mellitus (P<0.0001), family history of MI (P=0.001),

the body mass index (BMI) (P= 0.011), Cholesterol (P< 0.0001}, LDL (P=0.05) and fasting

blood glucose (P=0.0001). Total cholesterol, BMI, and FBS were statistically significant higher

in heterozygous patients comparing to heterozygous control subjects (CT) of PON1 alleles

(P=0.0014, 0.033, <0.0001, respectively). There was no statistically significant difference

reported between both groups as regarding other measured conventional risk factors [TGS,

HDL, LDL and VLDL (p>0.05)].

Our data suggests that the presence of (PON1) (rs 662) variant had no association with MI

among Egyptians living in Ismailia city. However the frequency of TT genotype is statistically

significantly higher in MI patients compared to controls, which may support our hypothesis

that carriers of the minor allele of PON1 have a higher risk for AMI, while carriers of the

major allele of PON1 have lower risk for AMI.



Pag

e3

8

Poster 13:

Establishment and Characterization of Inhibitors of the cGMP-

Dependent Protein Kinase type I

Sahar EM El-Deek 1, Soad Abdel Ghany1 , Atef Abo Alfadl 1, Robert Fiel 2, Maha M Esaam1

1Medical Biochemistry Department, Faculty of Medicine, Assuit University, Egypt

2Interfaculty Institute for Biochemistry, University of Tübingen, Germany

Atherosclerosis causes heart attack and stroke, the major causes of death all over the world.

The cGMP-dependent protein kinase I (PKG-I) is a potential mediator of nitric oxide (NO)

signaling in vascular smooth muscle cells (SMCs). Cell-fate mapping indicated that PKG-I is

involved in the development of SMC-derived plaque cells. Activation of smooth muscle PKG-I

contributes to the proatherogenic effect of NO and that inhibition of PKG-I might be a

therapeutic option for treating atherosclerosis.

Our aim is to establish specific inhibitors of PKG-I isoform, which include the N-terminal

domains of PKG-Iα or PKG-Iβ and to Investigate the interaction between these inhibitors and

the corresponding full length kinase.

Two fusion constructs consisting of enhanced green fluorescent protein (EGFP) and either

the N-terminal domain of PKG-Iα or PKG-Iβ were cloned and transfected to Mouse

embryonic fibroblast (MEFs) cells derived from mice based on two genetically different

mouse models, wild type and PKG-I knock out type and then the transfected cells were

analyzed by GFP fluorescence detection, SDS-PAGE, Western blot and Immunoprecipitation.

The results show that the two fusions constructs could be expressed by the MEF cells with

difference in the cellular localization, where the EGFP IαN is localized perinuclear while EGFP

IβN is localized inside the nucleus. The Immunoprecipitation had shown that there is

interaction between these inhibitors and the corresponding full length kinases so they had

been inhibited. On Conclusion: By the formation of these inhibitors we can inhibit the

pathogenesis of atherosclerosis and so the disease can be treated in human after further

investigations in vivo.

Key words: Atherosclerosis, PKG-Iα and PKG-Iβ



Pag

e3

9

Poster 14:

The Role of Antioxidant Hesperidin in Attenuation of Lung Cancer

Caused by Benzo(a)Pyrene in Balb/c mice.

Saad Abd Al-Jassabi 1, Mohd Syfiq Abdulla 2

1University of Kuala Lumpur, Faculty of Medicine, Malaysia

2University of Kuala Lumpur, Faculty of Pharmacy, Malaysia

It was postulated that chemoprevention is one of the most promising and realistic

approaches in the prevention of cancer. Hesperidin (HDN) is one such naturally occurring

flavonoid widely found in citrus fruits.

The aim of the present study is to divulge the chemopreventive nature of HDN during

Benzo(a)Pyrene (B(a)P) induced lung cancer in Balb/c mice.

Administration of B(a)P (60 mg/kg body weight) to mice resulted in increased lipid

peroxidation (LPO), lung specific tumor marker carcinoembryonic antigen (CEA) and serum

marker enzymes aryl hydrocarbon hydroxylase (AHH), alanine transaminase (ALT), with

concomitant decrease in the levels of tissue antioxidants like superoxide dismutase (SOD),

catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione.

HDN supplementation (25 mg/kg body weight) significantly attenuated these alterations

thereby showing potent anticancer effect in lung cancer. Overall, these findings substantiate

the chemoprotective piotential of HDN against chemically induced lung cancer in Balb/c

mice.

Keywords: Hesperidin, Antioxidant, Benzo(a)Pyrine, Flavonoids, Lung cancer



Pag

e4

0

Poster 15:

A Case of Familial Pseudohyperkalaemia with Genetic Analysis of the

ABCB6 Gene

EA Willis 1, M Khan

1, I Andolfo 2,3, A Iolascon 2,3, S Zouwail 1,4

1Department of Medical Biochemistry & Immunology, University Hospital of Wales, Heath Park, Cardiff, UK

2Department of Biochemistry and Medical Biotechnology, Università degli Studi di Napoli "Federico II"

3Ceinge, Biotecnologie Avanzate, Naples

4Department of Medical Biochemistry, School of Medicine, University of Alexandria, Egypt

Familial pseudohyperkalaemia (FP) is a rare asymptomatic autosomal dominant trait

affecting the temperature-dependent Na+/K+ATPase in erythrocytes. Individuals are

normokalaemic in vivo, but may demonstrate hyperkalaemia in vitro, due to passive leakage

of potassium across red cell membranes. Recently, ABCB6 was identified as the causative

gene of FP. We present a 41 year old Bolivian patient who presented with a serum

potassium of 8.3 mmol/L, following blood tests in the community. She had normal renal and

adrenal function, normal haematology and no changes on ECG.

In order to investigate for FP, bloods were collected from the patient, and left to stand at

different temperatures (4°C, room temperature, 37°C) for different time periods before

centrifugation and potassium analysis. Results showed a significant increase in potassium in

the samples left at 4°C and room temperature, compared with samples left at 37°C

suggesting a temperature-dependent flux of potassium from red blood cells in vitro. This

was consistent with a diagnosis of FP.

Subsequent direct sequencing analysis of the ABCB6 gene with specific oligonucleotide pairs

showed the mutation c.1361T>C; p.Val454Ala in homozygous state. The patient has been

advised that for future measurements of electrolytes, blood should be collected in hospital,

in order to minimize the time the blood is exposed to below in vivo temperatures.

Despite its rarity and benign nature, recognition of familial pseudohyperkalaemia is

important in order to avoid unnecessary hospital admissions, as well as the risk of

potentially inappropriate dangerous treatment for hyperkalaemia, or masking of a true

hypokalaemia.

Date post:	26-Mar-2016
Category:	Documents
Upload:	mahmoud-abbas
View:	223 times
Download:	9 times

BIOCHEM Cairo 2013 Abstract Book

Documents