by
Ivo Frébort
Protein electrophoresis
Polyacrylamide chemistryPolyacrylamide chemistry
acrylamide
3-15 %T
N,N’-methylene-bis-acrylamide
5 %C
polyacrylamide
S2O82- 2 SO4
-.
TEMED ( N, N,N´,N´-tetramethylethylendiamin)
Gel preparationGel preparation
Protein visualizationProtein visualization
ABS
600500400
Wavelength - nm
Coomasie bluewith proteinsbound
700
Coomasie blue
ABS
600500400
Wavelength - nm
Coomasie bluewith proteinsbound
700
Coomasie blue
Sensitivity 500 ng/band
Sensitivity 10-50 ng/band
Comassie blue stainig Silver stainig
CH2
N CH2
N CH2
R
CH2
N
n
n
R2
n = 2 nebo 3 R = H nebo -(CH2)n-COOH