C146-E227A
Ready-to-Use Analytical ConditionsShimadzu Method Packages deliver conditions for efficient and simultaneous multi-component analysis. They enable the user to quickly and easily implement complex methods without costly and laborious method development by providing sample preparation protocols, LC separation conditions, and MS acquisition parameters.
Ion pair or PFPP column methodA PFPP:Pentafluorophenylpropyl column method was newly adapted to expand your choice in separation conditions as well as target compounds.
Supporting LCMS-8030/8040/8050This method package supports the latest Shimadzu UFMS triple quadrupole mass spectrometer series, LCMS-8030, 8040 and 8050. LCMS-8050 delivers best-in-class sensitivity and the fastest scanning and polarity switching rates.
Complete from Sample Preparation to AnalysisProtocols are included for the preparation of extracts from biological tissue. Saving the user time and money, even laboratories unfamiliar with extraction can follow prescribed steps for LC/MS/MS sample preparation.
Normalization of Multiple Sample ResultsDue to mass differences between biological tissue samples, normalization of results must be performed when multiple samples are analyzed. This method package includes optimized analytical conditions for two internal standards to permit normalization across multiple samples.
LC/MS/MS Method Package for Primary Metabolites Ver. 2For LabSolutions Ver. 5
Overlaid MRM chromatograms for 55 standards mixture by ion pair methodArea ratio comparison between two tissues focused on methylation cycle
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0min
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
55000
60000 Erythrose-4-phosphateRibulose-5-phosphatePyruvateCMPNADDihydroxyacetonephosphateUMPGMP
LysineArginineHistidineGlycineSerineAsparagine
AlanineGlutamineThreonineCysteineL-Methionine sulfoneMethionine
TyrosineMES (2-Morpholinoethanesulfonic acid)GlutamateAspartatePhenylalanine
Glucose-6-phosphateRibose-5-phosphateSedoheptulose-7-phosphateFructose-6-phosphateTryptophanGlycerol-3-phosphateGlucose-1-phosphateGlyceraldehyde-3-phosphate
TMPAMPcGMPcAMPCDP6-PhosphogluconateGDPUDP3-Phosphoglycerate/2-PhoshoglycerateNADPFructose-1,6-bis-phosphateNADH
ADPTDPPhosphoenolpyruvateCTPGTPUTPATPTTPNADPH2,3-bis-PhosphoglycerateSuccinyl-CoAAcetyl-CoA
55 STDs Mix.
Methionine
S-Adenosylmethionine
Homocysteine
Cystathionine
Cysteine
Glutathione
Oxidized glutathione
Adenosine
Inosine
Uric acid
5-Glutamylcysteine
1 2
1 2
1 2
1 2
1 2
1 2
1 2
1 2
1 21 2
1 2
1 2
S-Adenosylhomocysteine
LCMS-8050
Index of compounds
Remarks and Precautions
1. For Research Use Only. Not for Use in Diagnostic Procedures.
2. Shimadzu makes no warranty regarding the accuracy of information included in the
database or the usefulness of information obtained from using the database.
3. It is the user’s responsibility to adopt appropriate quality control tests using standard
samples to confirm qualitative and quantitative information obtained with this method
package.
LC/MS/MS Method Package for Primary Metabolites Ver. 2For LabSolutions Ver. 5
© Shimadzu Corporation, 2014
2,3-Bisphosphoglyceric acid3-Phosphoglyceric acid(2-Phosphoglyceric acid)Dihydroxyacetone phosphateFructose 1,6-bisphosphateGlucose 1-phosphateGlucose 6-phosphateGlycerol 3-phosphatePhosphoenolpyruvic acidPyruvic acidFructose 6-phosphateGlyceraldehyde 3-phosphate6-Phosphogluconic acidErythrose 4-phosphateRibose 5-phosphateRibulose 5-phosphateSedoheptulose 7-phosphate
Acetyl coenzyme ASuccinyl coenzyme A
AlanineArginineAsparagineAspartic acidCysteineGlutamic acidGlutamineGlycineHistidineLysineMethioninePhenylalanineSerineThreonineTryptophanTyrosine
* The ion pair method is suitable for metabolites associated with a glycolytic system,
pentose-phosphate pathway and either ADP or ATP.
* The PFPP column method is good for covering amino acids, organic acids and metabolites
related to the methylation cycle and urea cycle.
List of compounds for ion pair method List of compounds for PFPP column method
Adenosine 3',5'-cyclic monophosphateAdenosine diphosphateAdenosine monophosphateAdenosine triphosphateCytidine diphosphateCytidine monophosphateCytidine triphosphateGuanosine 3',5'-cyclic monophosphateGuanosine diphosphateGuanosine monophosphateGuanosine triphosphateThymidine diphosphateThymidine monophosphateThymidine triphosphateUridine diphosphateUridine monophosphateUridine triphosphate
NADNADHNADPNADPH
2-Morpholinoethanesulfonic acidMethionine sulfone
Lactic acidPyruvic acid
2-Ketoglutaric acidAconitic acidCitric acidFumaric acidIsocitric acidMalic acidSuccinic acid
4-HydroxyprolineAlanineArginineAsparagineAspartic acidAsymmetric dimethylarginineCitrullineCystineDimethylglycineGlutamic acidGlutamineGlycineHistidineHomocystineIsoleucineLeucineLysineMethionine sulfoxideOrnitinePhenylalanineProlineSerineSymmetric dimethylarginineThreonineTryptophanTyrosineValineCystathionineCysteineHomocysteineMethionine5-GlutamylcysteineGlutathioneOxidized glutathioneS-AdenosylhomocysteineS-Adenosylmethionine
4-Aminobutyric acidAdenylsuccinic acidArgininosuccinic acidCholic acidCreatineNicotinic acidOphthalmic acidOrotic acidPantothenic acidTaurocholic acidUric acid
2-Aminobutyric acidAcetylcarnitineAcetylcholineAllantoinCarnitineCarnosineCholineCiticolineCreatinineCysteamineDopaDopamineEpinephrineHistamineHypoxanthineKynurenineNiacinamideNorepinephrineSerotonin
AdenineCytosineGuanineThymineUracilXanthineAdenosineCytidineGuanosineInosineThymidineUridineAdenosine 3',5'-cyclic monophosphateAdenosine monophosphateCytidine 3',5'-cyclic monophosphateCytidine monophosphateGuanosine 3',5'-cyclic monophosphateGuanosine monophosphateThymidine monophosphate
FADFMNNAD
2-Morpholinoethanesulfonic acidMethionine sulfone
Glyco
lytic system
Nu
cleotid
es
Org
anic acid
sN
ucleo
sides an
d N
ucleo
tides
Oth
ers
Co
-enzym
e
Am
ino
acids
Pento
se-ph
osp
hate
path
way
Inter-
nal
STDs
Glyco-
lyticsystem
Meth
ylation
and
Tran
ssulfu
ration
cycle
TCA
cycle
Am
ino
acids
TCA
cycle
Co
-en
zymes
Inter-
nal
STDs