CAN PESTICIDES & POTENCY BE ESTIMATED AT ONE SECOND/SAMPLEWITH A CELL PHONE & A MS?

Drew Sauter and Andrew D. Sauter IIInanoLiter LLCNanoliter.com

HD,NV 702-882-5413

Who is using rapid nanoLiter sample input now?

US DOE at Idaho National Lab, Lanthanides/Actinides.

US Army at Aberdeen Proving Ground MD. Highly toxic, VISCOUS liquids/agents.

University of Cincinnati, nucleosides, nucleotides and related entities.

Example customers, clients: U's of Ill, WI, CA, Cinn., MUSC, Wash. U., USF, USU, US Army APG, ECBC and Natick, Abbott, Biogen Idec, Genentech, Amgen, Hitachi, Allergan, Spark, Douglas, NIH, NIST, USDOE INL, Ga Tech, UNH, Duquesne, NASA, Air Force, Air Force, and Sciex offered to license. +. Agilent’s John Sausen request IBF information.

Patented Droplet Devices.

SAME Cell Phone Controller.M

ass Sp

ectrom

eter.

ESI MALDI

Fly droplets into MS! Fly droplets onto target for MALDI,SIMS & LDI.

DR

OP

LETS J. Harmon,

Make Great Crysytals

DROPLETS: IBF MS, Lanthanide Chelate From Idaho National Lab.*

Fastest, very efficient (100% or less), versatile and simplest MS sample introduction technology in the world! non-touch, low volume, dispensers, treatment devices for ESI or MALDI.

Droplets into MS’s,Rapid, INL, G. Groenewoldreproducible with 100% “all-in” !

Positive ion profiles generated by individual drops. a. Total ion current. b. M(NO3)2(CMPO)]+. c. [M(NO3)(CMPO)2]2+. Blue = Ce3+, Green = Tb3+, Red = Lu3+. * Bruker u-ToF, +eV, relflectron, 12 Hz, 50-2000 amu. (16).

DROPLETS

IBF-LC-MS/MS data of droplet introduction over a one minute acquisition window., 5, nucleosides (Cy, Ur, 5MeUr, Ad,2MeAd.), ASMS 2017, P. Limbach, et al. Dr. Ross celebrating. Device, nL Programmable Wave, an Android based source,

The intervals between droplet arrival in the mass analyzer are characterized by no background. More importantly, the relevantsample information is present within a single droplet. That is, an integrated mass spectra across droplets agrees with that from asingle droplet as previously published in Analytical Chemistry in 2013 by us and P. Limbach et al.

It is thought, but not proven, that this intensity variance shown by the yellow arrow and ? is because there was no cover over theinlet. Lots of other data shows excellent reproducibility. Data is + eV, conditions reported ASMS poster of Limbach, Sauter, et al.

DROPLETS: IBF MS/MS Infusion of Nucleosides, Nucleotides, RNA Hydrolysates, U. Cincinnati.

DROPLETS

Dr. Ross

DROPLETS: Shoot “cells & cellular” liquids into an MS systems. EPA LV.100% reproducible cell/saliva sample introduction into an ESI/MS ! Biomarker, direction discovery tool ?

Mass spectra from three 150 nL droplets containing white matter, cells, human saliva.

Spectra called “exciting” by America’s greatest mass spectroscopist!

Can IBF of cells, fragments efficiently direct biomarker discovery?

I believe so and I’m seeking cancer, disease R&D collaborators.

NIH considering doing bacteria this way, may not need a laser or may have an alternate approach.

100 % into MS !

Adjacent, healthy

cells

Cancer, disease

cells

DROPLETS

Dr. Grange

DROPLETS: Single drops from an OPERATING! Li+ battery electrolyte + fire retardants, DOE INL.

Dr. G. S. Groenewold, et al, Idaho National Lab. Very early input by Drew Sauter nanoLiter LLC.

• Coin cells: dependent on Li+ transport in carbonate electrolyte – weakly coordinating solvents

• EMC

• EC

• Breakdown can lead to H2 production, flammability issues

500 550 600 650 700 750 800

0.0

1.0x104

2.0x104

3.0x104

4.0x104

5.0x104

662, [(

3,3

)Li(E

C)]

+

786, [(

1,5

)Li(E

MC

)]+

780, [(

1,5

)H(E

MC

)]+

726, [(

2,4

)H(E

MC

)]+

678, [(

3,3

)Li(E

MC

)]+

520, [(

4,2

)H]+

624, [(

4,2

)Li(E

C)]

+

538, [(

4,2

)Li(H

2O

)]+

592, [(

3,3

)Li(H

2O

)]+

460, [(

5,1

)H]+

608, [(

4,2

)Li(E

C)]

+

520, [(

4,2

)Li]+

484, [(

5,1

)Li(H

2O

)]+

712, [(

4,2

)Li(E

C) 2

]+

564, [(

5,1

)H(E

MC

)]+

574, [(

3,3

)Li]+

466, [(

5,1

)Li]+

60 64 68 72 76 80 84

0.0

1.0x104

2.0x104

3.0x104

4.0x104

5.0x104

6.0x104

7.0x104

8.0x104

Extracted ion chromatograms, 100 nL droplets

[(4,2)H]+

[(4,2)Li]+

inte

nsity

time (sec)

538

520

514

[(4,2)Li(H2O)]

+

(Ethoxy)x(2,2,2-triflurorethoxy)yphosphazene cyclotrimers(x,y) short hand nomenclature

Lithium ion battery w/fire retardants

ESIMS,MS,IMS

DROPLETS into an n ESI! HRMS of nanoLiter droplets!

100% Shot into MS !

FedExs to Elon Musk!

DROPLETS for US ARMY APG-X1 DEVICE: INTO/ONTO TARGETS. CLASSFIED WORK.

Fly’s nLs to targets of even viscous liquids in classified projects and other efforts onto or into targets! APG,MD Army validation from 5 – 500 nLs. ASMS 2016. Drs. Oyler and Koplovits, et al, Note: GoPro® too!

THE PROGRAMMABLE WAVE ALSO MAKES EXCELLENT MALDI, SIMS, LDI CRYSTALS VIA THE SAME IBF DEVICE!IT ALSO HAS WET LAB APPLICATIONS, e.g., CRYSTALLOGRAPHY, TLC, and OTHER VERY COOL APPS (OVER).

Drew Sauter, nanoLiter LLC, 217 Garfield Drive, Henderson, NV, 89074, USA, nanoliter,com, adsauterjr@gmail.com.

IF CHROMATOGRAPHY NEEDED.

THE nL PROGRAMMABLE WAVE= 100% INPUT EFFICIENT ESI UPLC MS. ANDROID CONTROLLED

ALLOWS YOU TO CREATE & CONTROL DROPLET TIMING, ENERGY, WAVE FUNCTION, VOLUME, POLARITY & LOCALE.

A POWERFUL VERY RAPID ESI LCMS & ESI INFUSION R&D DEVICE! WE CONSULT TOO!

Extracted ion chromatograms of nucleosides, top, cytidine, uridine, 5-methyluridine, adenosine, and 2’-O-methyladenosine separated on a PGC capillary column and introduced into the MS by inductive charging. per Asilomar 2016 at U. of Cincinnati with Drs. Ross & Limbach. Also, see P. Limbach, et al , JMS Oct. 2015.

A. Sauter III

m/z 244

m/z 245

m/z 259

m/z 268

m/z 282

* IBF. It’s speed. It’s plenty fast enough! > 1 Hz,100% sample introduction efficiency in milliseconds possible.

* Ion trap, simple might be possible! QQQ CID or HRMS if needed!

* Solves the N & sampling problem. You can acquire analysis variance!

* Isobaric interferences a big problem?1 Second MRM Droplet integration!?Alternate analytes or scans types.Parallel analysis.Reactive, other CID.SPE, Really Rapid chromatography?Other ?

Pics & video clips.

10 Slide Overview

Cool Video,

5 sec IBF, ESI sample input, INL

0.5 sec IBF,ESI sample input,INL

1.0 sec, IBF, ESI sample input UCSD.

Robot/MALDI & LC/MALDI

nL Programmable Android Dispenser,

IBF UPLC MS.

More at nanoLiter.com

ISSUES REGARDING PATENTED RAPID ( 1 to 10 SECOND/SAMPLE) POTENCY SREENING. ARMY, DOE, MAJOR U’s, AIR FORCE & OTHERS USING THE TECHNOLOGY ACROSS THE USA.

*DOABLE? I BELIEVE SCREENING CAN BE DONE GIVEN THE INCENTIVE.

NEW DEMO!Fastest Nevada Pesticide SCREEN

2 Second/Sample via IBF Droplets.

By

SIS, Caltech & nL LLCDrew Sauter 702-882-5413

C:\Users\...\Desktop\Sauter_180823\DS-3 8/23/2018 10:38:17 AM unknown

RT: 0.00 - 2.39

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2.0 2.1 2.2 2.3

Time (min)

0

10

20

30

40

50

60

70

80

90

100

Re

lative

Ab

un

da

nce

0.85

1.65

1.33

2.13

0.660.59 0.950.24 0.790.50 1.75 1.970.310.11 0.37 1.20 1.841.07 1.14 1.49 2.292.23

NL:

3.92E6

TIC MS

DS-3

DS-3 #25-58 RT: 0.79-1.84 AV: 34 NL: 1.66E4

T: + p ESI Full ms [150.00-1500.00]

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

m/z

0

10

20

30

40

50

60

70

80

90

100

Re

lative

Ab

un

da

nce

837.27

821.40

815.13

408.27

460.07211.87

885.53 952.07 1010.00

623.07

1208.531171.67

383.93 675.60

1377.93

713.07

1103.40

590.27224.53

1342.93 1478.80

355.27 746.87

1265.73

508.20253.33563.60292.13206.67

x30

25 analytes composite Nevada pest std_150ms3 Droplets: Handheld Device_1st Attempt @ Caltech.

C:\Users\...\Desktop\Sauter_180823\DS-23 8/23/2018 1:01:39 PM std direct intro phone control 5 ms 1 a

RT: 0.18 - 0.41

0.18 0.20 0.22 0.24 0.26 0.28 0.30 0.32 0.34 0.36 0.38 0.40

Time (min)

0

10

20

30

40

50

60

70

80

90

100

Relat

ive Ab

unda

nce

0.30

0.25

0.28

0.200.33 0.35 0.38

0.23

0.180.40

NL:

9.18E9

TIC MS

DS-23

DS-23 #23-46 RT: 0.19-0.39 AV: 24 NL: 7.05E7

T: + p ESI Full ms [100.00-900.00]

150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900

m/z

0

10

20

30

40

50

60

70

80

90

100

Relat

ive Ab

unda

nce

837.27

408.33

430.47

631.00

880.47

666.20520.33744.53538.07444.00 714.40 760.47591.60

294.20

814.53468.33

334.27

382.27237.27 290.93172.93

130.07 371.20200.87

127.80

x50 x10

8 , 50 nL droplets, 1 PPM, 25 analytes composite pesticide std_5ms. 2 Seconds/sample Nevada Pesticide SCREEN!

LCQ MS nanoLiter Programmable Android Dispenser. 75% fs, 1 sec time, BP, Linear, SIS pump, IBF sryringe

8 , 50 nL droplets

Doing Science & Business.

Developing Very Rapid MS Screening and Analysis Technology & Methods.

Seeking Licensed Marijuana collaborators, growers?

Drew Sauter, President, nanoLiter LLC 217 Garfield Drive, Henderson, NV, 89074, USA, 702-882-5413, adsauterjr@gmail.com, nanoliter.com

Video clipsCool Video, 5 sec input, 0.5 sec input, 1.0 sec UCSD Data, nL Programmable, IBF UPLC MS.