5. Sera•

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Special Fetal Bovine SeraStandard Fetal Bovine SeraOther Sera

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Sera The production process starts with the collection of the raw material, based on a closed sterile bag system to avoid bacterial contamination which would result in the presence of endotoxins. Serum is transported and stored at -20 °C, the temperature is monitored so that any anomaly can be traced; quality and sterility are guaranteed.The raw serum is collected from EU and USDA approved areas. The USDA approved areas are Australia, New Zealand, USA and central American countries; while the EU approved areas are mainly Latin

American countries.The quality of our sera is checked at each step of the production process; Serum is filtered through a series of three 0.1 µm pore-size filters.Sera are manufactured according to relevant current Good Manufacturing Practices (cGMP) guidelines. All raw materials are certified (USDA or EU approved) and each batch is sample tested by independent laboratories. All the products are controlled for the presence of virus and mycoplasma.

Heat-inactivation

Heat-inactivation (heating at 56°C for 30 min) is done to inactivate complement, a group of proteins present in sera that are part of the immune response. This is sometimes important for cells that will be used to prepare or assay viruses, used in cytotoxicity assays or other systems where complement may have an unwanted influence. Years ago heat was used to destroy mycoplasma in serum. Nowadays EuroClone and most serum suppliers filter sera through three consecutive 0.1 µm pore-size filters to remove mycoplasma before distribution. Heat-inactivation is still recommended for growing embryonic stem cells (p. 75 in Rudnicki, M.A., and McBurney, M.W. (1987) Teratocarcinomas and Embryonic Stem Cells- A Practical Approach (IRL Press Ltd., Oxford)), as well as for many insect cell lines (Weiss, S.A., et al. (1995). Meth. Mol. Biol. 39:65). It has been reported that heat inactivation will reduce or destroy serum growth factors, and should only be done when there is a compelling reason (Girard, D.J. (1987), In vitro cellular and developmental Biology 23, 691-697).

Technical TIPS:

Thaw serum : Remove the serum from frozen storage and place it overnight in a refrigerator at 2°C to 6°C. Transfer the bottles to a 37°C water bath. Swirl the bottles from time to time in order to mix the solutes that tend to concentrate at the bottom of the bottle. Do not keep the serum at 37°C any longer than necessary to completely thaw it. Thawing serum in a bath above 40°C without mixing may lead

to the formation of a precipitate inside the bottle. We don’t recommend thawing the serum at high temperature.

The procedures used to prepare serum may retain some fibrinogen. Since external factors may initiate the conversion of fibrinogen to fibrin, flocculent material or turbidity may be observed after thawing. Testing of serum after this has happened indicates that it does not alter its ability to function as a supplement for cell culture media. If the presence of flocculent material or turbidity is a concern, it can be removed by filtration through a 0.45 µm filter. A precipitate can form in serum that is incubated at 37°C for prolonged periods of time. Electron microscopy and X-ray microanalysis indicate that the precipitate may include crystals of calcium phosphate. The formation of a calcium phosphate precipitate does not alter the performance of the serum as a supplement for cell culture.

Special Fetal Bovine Sera

Pharma Grade FBS

FBS Pharma Grade offers the customer the highest of safety particularly with regard to possible virus contamination. The production process is governed by the current biopharmaceutical guidelines and the final product is correlated by documentary traceability.Pharma Grade FBS is gamma irradiated at 35 kGy. The use of FBS Pharma Grade is suggested for many applications, vaccine and proteins production, advanced new cell therapies, etc.

Features 100nm filtered Tested according to all current biopharmaceutical guidelinesAustralian OriginNo BVD antibody interferencesGamma irradiation in single boxes at 35kGyManufactured according cGMPComprehensive documentary traceabilityShelf life 5 years Store at -20°C

ApplicationVaccine and recombinant proteins productionAdvanced new cell therapies

Cat.No. Description VolumeECS0172D ECS0172L

FBS PHARMA GRADE 100 ml 500 ml

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Tetracycline Screened FBS

Tetracycline Screened FBS is designed for expression studies in Tet-on/Tet-off systems and transfection, the main feature are batches with undetectable level of tetracycline derivates, low endotoxin and IgG content.

FeaturesEU approved100nm filteredBatches with undetectable level of any tetracycline derivatesIdeal for researchers using TETonTEToff systemSuitable for transfection and expression studiesHigh growth capacityLow IgG contentShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0182DECS0182L

FBS Tetracycline Screened100 ml 500 ml

Embrionic Screened - Euromed ES FBS

Embrionic Screened Euromed ES FBS is specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cell. The screening includes colony morphology, toxicity tests and plating efficiency. The level of endotoxin is < 1 EU/ml. The use of Embrionic Screened Euromed ES FBS is suggested to support the growth of undifferentiated colonies of ES cells.

Features Embryonic Stem (ES) cell screened100nm filteredNo prescreening neededBatches are screened in our facility by experienced ES-cell specialistsIndifferentiated cell growth guaranteedEndotoxin level ≤ 1EU/mlShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0196DECS0196L

Euromed ES FBS 100 ml 500 ml

Heat Inactivated

FBS Heat Inactivation is performed at 56°C for 30 minutes with consequent inactivation of complement system. The use of Heat Inactivated FBS is suggested for supporting most cell types.

FeaturesHeat inactivation treatment is carried out in a controlled environment to reproduce a consistently accurate heat inactivationThis treatment destroys the complement proteinsHeat Inactivation performed at 56°C for 30 minsEU approved: Origin depends on batch number (mainly Latin America) USDA approved: Origin depends on batch number (mainly Mexico origin)100nm filteredShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0180DH ECS0180LH

FBS EU approved 100 ml 500 ml

ECS0170DH ECS0170LH

FBS Australian Origin 100 ml 500 ml

ECS0120DH FBS USDA approved 100 mlECS0120LH 500 ml

Gamma Irradiated

FBS Gamma Irradiated is irradiated at 25-35 kGy with reduction or elimination of virus and bacteria.The use of FBS Gamma Irradiated is suggested for virus and vaccine productions, on biopharmaceutical productions and during the manufacture of diagnostic products.

FeaturesGamma irradiation at 25-35kGyThis treatment destroys most viruses and bacteriaEU approved: Origin depends on batch number (mainly Latin America) USDA approved: Origin depends on batch number (mainly Mexico origin)100nm filteredShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0180DI ECS0180LI

FBS EU approved 100 ml 500 ml

ECS0170DI ECS0170LI

FBS Australian Origin100 ml 500 ml

ECS0120DI FBS USDA approved 100 mlECS0120LI 500 ml

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Dialysed FBS Dialysed is producted by diafiltration process, that removes many hormones and reduces the content of nucleotides and amino acids. The cut-off is 10KDa against a 0.15 M physiological saline solution.The use of FBS Dialysed is suggested for hormone free cell culture, minimal essential growth supplement and incorporation studies.

FeaturesThis treatment removes some growth factorsMainly used for special applications100nm filteredShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0171D ECS0171L

FBS Australian Origin100 ml 500 ml

Standard Fetal Bovine SeraEuroClone Standard Fetal Bovine Sera are characterized by high quality, sterility and homogeneity; they are used as the main source for growth factors, vitamins, hormones, attachment and transport factors and other cell stimulating components. The raw sera are collected from EU and USDA approved areas.EuroClone preselects the lots and offer its customers only the best performers one.

Features

EU approved: Origin depends on batch number (mainly Latin America) USDA approved: Origin depends on batch number (mainly Mexico origin)100nm filteredShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0180D ECS0180L

FBS EU approved 100 ml500 ml

ECS0170DECS0170L

FBS Australian Origin100 ml 500 ml

ECS1102D ECS1102L

FBS High QualityUS Origin

100 ml500 ml

ECS0120D FBS USDA approved 100 mlECS0120L 500 ml

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Bovine Serum

FeaturesShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0070D ECS0070L

Newborn Calf Serum Serum origins from calves up to 10 days old

100 ml 500 ml

ECS0040D ECS0040L

Calf Serum origins from animals which are not more than 12 months old

100 ml 500 ml

ECS0020D ECS0020L

Bovine Serum originates from cattle which are more than 12 months old

100 ml500 ml

Other Sera

Human Serum

Human Serum Off the clot is obtained from whole blood which has not been treated with anticoagulants. It goes through the natural blood clotting process and only then is it centrifuged, so removing the solid constituents of the blood together with the fibrin. It is particularly suitable for culturing human lymphocytes and macrophages.It contains neither anti-A nor anti-B antibodies.

Serum is transported and stored at - 20 °C, the temperature is monitored so that any anomaly can be traced; quality and sterility are guaranteed.Each batch is rigorously controlled, from the collection of serum by our own staff and in all the stages of its treatment and production on our premises through to final packaging.As all the other EuroClone sera, Human serum is manufactured according to relevant current Good Manufacturing Practices (GMP) guidelines.The use is suggested for sensitive cell culture, it is suitable for most human cells and it is specially recommended for lymphocytes and human macrophages.

FeaturesFrom whole blood without anticoagulantSingle-use blood donor bagsNo anticoagulant addedNaturally clottedCentrifugedWithout fibrinWithout AB antibodies200nm filteredStore at -20°C

Cat.No. Description Volume

ECS0219DHuman Serum AB HIV, HBsAg and HCV tested

100 ml

Serum From Other Species

FeaturesEU approvedShelf life 5 yearsStore at -20°C

Cat.No. Description VolumeECS0090D ECS0090L

Donor Horse Serum Obtained from donor animals

100 ml 500 ml

ECS0091D ECS0091L

Horse Serum 100 ml 500 ml

ECS0050D ECS0050L

Chicken Serum 100 ml 500 ml

ECS0200D ECS0200L

Goat Serum 100 ml 500 ml

ECS0230D ECS0230L

Lamb Serum 100 ml 500 ml

ECS0240D ECS0240L

Porcine Serum 100 ml 500 ml

ECS0250D Rabbit Serum 100 ml