CCulture Growth Mediaulture Growth Media Growth Media Final.pdf · 2013-01-04 · Fig 1. pBK...

Culture Growth MediaCulture Growth Media

Bacteria

YeastMammalian Plant

One Call. One Source.A World of Culture Growth Media.

Product Guide

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…....BACTERIA...YEAST...PLANT...MAMMALIAN CELLS...BACTERIA...YEAST...PLANT...M

CULTURE GROWTH MEDIA

MP Biomedicals growth media are tested for pH, solubility, clarity, purity as well as cell density and plasmid yield suitable to the appropriate strains. Quality assurance and validation procedures ensure lot-to-lot consistency and performance.

Convenient Packaging for Various Needs

Highest Quality for Molecular Biology Applications

A Unique Range of Media from One Source

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For phone or email orders, call (65) 6775 0008 or email to [email protected]

T...MAMMALIAN CELLS...BACTERIA...YEAST...PLANT...MAMMALIAN CELLS...BACTERIA

- Unparalleled Quality and Selection

MP Biomedicals offers a complete line of standard and specialty culture media to meet your various needs. We specialize in formulations for bacterial, yeast, plant tissue and mammalian cell culture applications. We also have more than a thousand recipes and variations!

MP Biomedicals is the exclusive supplier of encapsulated growth media providing ultimate ease and convenience. Eliminate the waste, inaccuracies, and mess associated with weighing out bulk powder! Simply drop the capsules in water and autoclave! In addition tocapsule, we also offer pre-measured powder in pouches, liquid media and pre-poured plates

Custom Formulations Made to Your Specifi cations

MP Biomedicals can supply growth media that are customized for a specifi c organism or research application on any scale. Can’t fi nd the type of media you need? We can make a custom recipe for you with our high quality raw materials following strict manufacturing guidelines and Good Laboratory Practices (GLP).

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CIRCLEGROW®

Superior Plasmid Yield Through Enhanced Cell Growth

Perfect for overnight or weekend cultures, CIRCLEGROW® Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium.

CIRCLEGROW® Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth.

Cells grown with CIRCLEGROW® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed.

Reduce Hands-On Work and Preparation Time

CIRCLEGROW® cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confi dently leave cultures overnight or over the weekend.

CIRCLEGROW® capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW® is also available in bulk powder, single-use pouches, capsules and sterile liquid.

Bacteria grown in conventional culture media, L-Broth, reach an abrupt stationary phase upon exhaustion of nutrients. Cell proliferation is halted completely and some cell death and plasmid loss occurs. At the end of log phase, cells growing in CIRCLEGROW® begin to utilize a proprietary second carbon source which becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase. Bacteria commonly grow in CIRCLEGROW® to a density triple that achieved in other media.

CIRCLEGROW® BrothUse 40 grams (40 capsules) per liter.Size Cat. No.

Powder

227 g (0.5 lb) 113000112

454 g (1 lb) 113000122

908 g (2 lb) 113000132

2.3 kg (5 lb) 113000142

5 kg (11 lb) 113000152

15 kg (33 lb) 113000162

Pouches

10 x 0.5 L 113000165

100 x 0.5 L 113000185

10 x 1 L 113000175

Capsules

250 caps 113000121

1000 caps 113000131

2500 caps 113000141

Sterile Solution

500 mL 113000104

Cel

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Cell Growth of E. coli

Bacterial Media

CIRCLEGROW

L-Broth

4 8 12 16

107

109

1010

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Bacterial Media

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LB MediumLB broth is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use.

Formulations for the E. coli growth media known as LB Medium or L-Broth typically contain 10 g of tryptone and 5 g of yeast extract per liter, and can vary in salt concentration from 0.5 g to 10 per liter as follows:

• LB Medium contains 10 g NaCl per liter• Lennox LB Medium contains 5 g NaCl per liter• L-Broth contains 0.5 g NaCl per liter

LB-Agar MediumUse 40 grams (40 capsules or 8 large capsules) per liter.

Contents/liter: LB Medium, 15 g agar

Size Cat. No.

Powder

454 g (1 lb) 113002222

1 kg (2.2 lb) 113002232

Pouches

10 x 0.5 L 113002265

10 x 1 L 113002275

Capsules

227 g (0.5 lb) 113002211

454 g (1 lb) 113002221

1 kg (2.2 lb) 113002231

5 kg (11 lb) 113002241

Large Capsules

227 g (0.5 lb) 113002216

454 g (1 lb) 113002226

1 kg (2.2 lb) 113002236

5 kg (11 lb) 113002246

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Bacterial Media

Now available!2012-2014 Catalogmpbio.com/catalog

LB MediumUse 25 grams (25 capsules or 4 large capsules) per liter.Contents/liter: 10 g tryptone, 5 g yeast extract, 10 g NaCl

Size Cat. No.

Powder

454 g 113002022

1 kg (2.2 lb) 113002032

5 kg (11 lb) 113002042

Pouches

10 x 0.5 L 113002065

100 x 0.5 L 113002085

10 x 1 L 113002075

100 x 1 L 113002095

Capsules

227 g 113002011

454 g (1 lb) 113002021

1 kg (2.2 lb) 113002031

5 kg (11 lb) 113002041

15 kg (33 lb) 113002051

Blister Pack Capsules

250 g 113002011B

Large Capsules

227 g (0.5 lb) 113002016

454 g (1 lb) 113002026

1 kg (2.2 lb) 113002036

5 kg (11 lb) 113002046

Sterile Solution

500 mL 113002014

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Lennox LB MediumUse 20 grams (20 capsules or 4 large capsules) per liter.Contents/liter: 10 g tryptone, 5 g yeast extract, 5 g NaCl

Size Cat. No.

Powder

454 g (1 lb) 113002122

1 kg (2.2 lb) 113002132

5 kg (11 lb) 113002142

Capsules

454 g (1 lb) 113002121

1 kg (2.2 lb) 113002131

5 kg (11 lb) 113002141

Large Capsules

1 kg (2.2 lb) 113002136

L-Broth AgarUse 31 grams (31 capsules) per liter.

Contents/liter: L-Broth, 15 g agar

Size Cat. No.

Capsules

454 g (1 lb) 113001221

1 kg (2.2 lb) 113001231

LB Medium

L-BrothUse 16 grams (16 capsules) per liter.

Contents/liter: 10 g tryptone, 5 g yeast extract,0.5 g NaClSize Cat. No.

Powder

1 kg (2.2 lb) 113001032

Capsules

454 g (1 lb) 113001021

1 kg (2.2 lb) 113001031

Lennox LB AgarUse 35 grams (35 capsules or 6 large capsules) per liter.

Contents/liter: Lennox LB Medium, 15 g agar

Size Cat. No.

Powder

1 kg (2.2 lb) 113002332

Capsules

1 kg (2.2 lb) 113002331

Large Capsules

454 g (1 lb) 113002326

1 kg (2.2 lb) 113002336

Bacterial Media

The Ultimate in Ease and Convenience

Encapsulated Culture Growth Media

Exclusive from

MP Biomedicals

No weighing. No dust. No cleanup. No smell. Simply drop capsules in water and autoclave Unbeatable selection of formulations Also available in other pre-measured formats

Custom media formulations available upon request.

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GS96 BrothUse 50 g/L

Size Cat. No.

Powder

227 g (0.5 lb) 113101112

1 kg (2.2 lb) 113101132

GS96 Broth

High Yields of Plasmid DNA in 96- and 384-well plates

GS96 Broth is formulated to grow host cells to the highest density possible without aeration and to produce high yields of plasmid DNA. Perfect for growing bacterial cells in 96-well and 384-well plates, GS96 media can be used in any situation in which aeration of the culture is diffi cult or absent. Cell density and plasmid yield can be further enhanced up to 50% by adding 0.05% glycerol. Comparison studies with other media, such as LB, show that the use of GS96 in non-shaking culture results in superior cell growth and plasmid yields.The increase in host cell density and overall plasmid yield varies between different host/plasmid systems (Fig 1, 2, & 3).

Growth and plasmid yield of three E. coli strains in GS96 and LB media (Figures 1, 2 and 3 above).Cultures of 1.5 mL were grown in 2 mL microcentrifuge tubes. The tubes were capped and the samples were incubated at 37°C overnight without shaking. Cell density was measured by OD600 and plasmid DNA was isolated using the RapidPure® Plasmid Mini Kit (Cat. No. 112066200). A 5 µL aliquot of the DNA isolated from each sample was run on a 1% gel. DNA was tested and found to be an excellent template for DNA sequencing.

Fig 1. pBK RSV/XL1-Blue

Fig 2. MPVII mega/DH5 α

Fig 3. pCHIIc/JM109

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cell density(OD600)

AveragePlasmid

Yield

LB 0.379 1.2 µg

GS96 + Glycerol 1.160 3.2 µg

GS96 0.880 2.1 µg

cell density(OD600)

AveragePlasmid

Yield

LB 0.620 0.9 µg


GS96 1.239 1.5 µg

Hindlll

cell density(OD600)

AveragePlasmid

Yield

LB 0.533 0.9 µg


GS96 1.079 1.7 µg

Hindlll

Bacterial Media

For phone or email orders, call

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Competent Cell Preparation & Growth Media

Terrifi c BrothPart 1: Use 36 grams (36 capsules) per liter.

Contents/liter: 24 g yeast extract, 12 g tryptonePart 2: Potassium phosphate salts and glycerol in sterile solution

Size Cat. No.

Powder, part 1 (includes Part 2 sterile liquid)

1 kg (2.2 lb) 113045032

Capsules, part 1 (includes Part 2 sterile liquid)

1 kg (2.2 lb) 113045031

Dehydrated Media

500 g 091012017

Terrifi c Broth IITerrifi c Broth II is a proprietary formulation that is shipped as

a single powder instead of two separate components. Terrifi c Broth II exhibits the same growth characteristics as

the original Terrifi c Broth formula.Use 50 grams (50 capsules) per liter.Contents/liter: proprietary formulation

Size Cat. No.

Powder

454 g (1 lb) 113046022

1 kg (2.2 lb) 113046032

5 kg (11 lb) 113046042

Pouches

10 x 0.5 L 113046065

10 x 1 L 113046075

Capsules

454 g (1 lb) 113046021

1 kg (2.2 lb) 113046031

5 kg (11 lb) 113046041

Terrifi c Broth, Modifi edSuitable for the cultivation of molecular genetic strains of E.

coli and increased plasmid yield.Contents/liter: 12 g tryptone, 24 g yeast extract,

2.2 g monopotassium phosphate, 9.4 g dipotassium phosphate

Size Cat. No.

Powder

500 g 091010417

SuperbrothUse 60 grams (60 capsules) per liter.

Contents/liter: 35 g tryptone, 20 g yeast extract, 5 g NaCl

Size Cat. No.

Powder

1 kg (2.2 lb) 113010032

5 kg (11 lb) 113010042

Pouches

100 x 1 L 113010095

Capsules

1 kg (2.2 lb) 113010031

Superbroth AgarUse 75 grams (75 capsules) per liter.Contents/liter: Superbroth, 15 g agar

Size Cat. No.

Capsules

1 kg (2.2 lb) 113010231

SUPER-COMP Media and Transformation Salts

SUPER-COMP is an extremely rich medium that signifi cantly increases the competency of the cells when using a simple

RbCl2/CaCl2 means of preparing competent host cells.

Description Size Cat. No.

Powder

SUPER-COMP Media 100 caps 113103011

10 x RbCl/CaCl Transformation Salts

150 mL 113103014

SOB MediumStandard media for preparing cells for chemical

transformation Use 31 g/L

Contents/liter: 20 g tryptone, 5 g yeast extract, 0.5 g NaCl, 5 g MgSO4•7H20

Size Cat. No.

Powder

454 g (1 lb) 113030022

Bacterial Media

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Bacteriophage Growth Media

2xYT-BrothUse 31 grams (31 capsules or 6 large capsules) per liter.Contents/liter: 16 g tryptone, 10 g yeast extract, 5 g NaCl

Size Cat. No.

Powder

454 g (1 lb) 113012022

1 kg (2.2 lb) 113012032

5 kg (11 lb) 113012042

Capsules

454 g (1 lb) 113012021

1 kg (2.2 lb) 113012031

5 kg (11 lb) 113012041

Large Capsules

5 kg (11 lb) 113012046

2xYT-Broth AgarUse 46 grams (46 capsules) per liter.

Contents/liter: 2xYT, 15 g agar

Size Cat. No.

Capsules

1 kg (2.2 lb) 113012231

YT-BrothUse 18 grams (18 capsules or 4 large capsules) per liter.Contents/liter: 8 g tryptone, 5 g yeast extract, 5 g NaCl

Size Cat. No.

Capsules

1 kg (2.2 lb) 113011031

Large Capsules

5 kg (11 lb) 113011046

NZYM Broth (NZY) Use 22 g/L.

Content/liter: 10 g NZ-amine, 5 g yeast extract, 2 g MgSO4• H2O, 5 g NaCl, pH 7

Size Cat. No.

Powder

454 g (1 lb) 113005022

General Microbiological Media

Malt Extract AgarSuited for the cultivation and enumeration of yeasts and

fi lamentous fungi (mold) from cosmetics and other samples.Use 33.6 g/L

Contents/liter: 12.75 g malt extract, 2.75 g dextrin, 2.35 g glycerin, 0.78 g gelatin peptone, 15 g agar

Size Cat. No.

Powder

500 g 091006817

Potato Dextrose BrothSuited for the cultivation of a variety of yeasts and molds

from dairy products and other foods. Use 24 g/L

Contents/liter: 4 g potato infusion, 20 g dextrose

Size Cat. No.

Powder

500 g 091008617

YT media formulations are optimized for the growth and maintenance of M13 phage or other fi lamentous ssDNA bacteriophages.

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Bacterial Media

MP Biomedicals offers media and supplements for the growth,maintenance, and isolation of various microorganisms.

Dulbecco’s Modifi ed Eagle’s Medium

Size Cat. No.

500 mL 091642954

DMEM is a basal medium consisting of typical amino acids, glucose, pH indicator, salts, vitamins, cystine and tyrosine (does not contain L-glutamine and inositol). A higher glucose level (4,500 mg/L) has proven to be optimal for cultivating several different cell types. Absence of growth-promoting agents, antimicrobials or proteins in the media gives freedom to adjust the concentration according to the cell type.

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MP Biomedicals yeast media are available in an exceptional variety of formulations. Take the confusion out of selectively growing your yeast cells by taking advantage of our years of expertise! Our wide range of yeast nitrogen bases, sugars, and amino acids dropout mixtures can be mixed together to form almost any complete media imaginable. Many of these combinations are already available off-the-shelf as pre-mixed powders.

Growth Media for Saccharomyces cerevisiaeMP Biomedicals offers both YPD and YEP standard media in broth and agar formulations. YPD contains dextrose, while YEP contains only peptone and yeast extract. YEP is therefore useful for yeast applications requiring an alternative or non-fermentable carbon source. Pre-measured capsules or pouches, as well as bulk powder packaging, are available to suit the needs of every laboratory.

Visit our website for moreproduct information

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YEP BrothUse 30 grams (6 large capsules) per liter.

Contents/liter: 20 g peptone, 10 g yeast extract, pH 6.5 at 300C

Size Cat. No.

Powder

1 kg (2.2 lb) 114004032

Large Capsules

5 kg (11 lb) 114004046

YPD (YEPD) AgarUse 67 grams (67 small capsules) per liter.

Contents/liter: YPD, 17 g agar

Size Cat. No.

Powder

1 kg (2.2 lb) 114001232

Pouches

10 x 1 L 114001275

Small Capsules

1 kg (2.2 lb) 114001231

YPD (YEPD) BrothUse 50 grams (10 large capsules) per liter.

Contents/liter: 20 g peptone, 10 g yeast extract, 20 g dextrose pH 6.5 at 300C

Size Cat. No.

Powder

454 g (1 lb) 114001022

1 kg (2.2 lb) 114001032

5 kg (11 lb) 114001042

Pouches

10 x 1 L 114001075

Sterile Solution

1 L 114001034

Large Capsules

1 kg (2.2 lb) 114001036

Yeast Media

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Yeast MediaSynthetic Defi ned Yeast Media

Description mg/L

Potassium Phosphate monobasic 1000.000

YNBYeast Nitrogen

Base

SDSynthetic Defi ned

Magnesium Sulfate 500.000

Sodium Chloride 100.000

Calcium Chloride 100.000

Biotin 0.002

Calcium Pantothenate 0.400

Folic Acid 0.002

Inositol 2.000

Niacin 0.400

p-Aminobenzoic Acid 0.200

Pyridoxine HCl 0.400

Ribofl avin 0.200

Thiamine HCl 0.400

Boric Acid 0.500

Copper Sulfate 0.040

Potassium Iodide 0.100

Ferric Chloride 0.200

Manganese Sulfate 0.400

Sodium Molybdate 0.200

Zinc Sulfate 0.400

Total 1705.844

Ammonium Sulfate 5000.000

Dextrose 20000.000

Total 26705.844

Adenine 10.000

L-Arginine HCl 50.000

L-Aspartic Acid 80.000

L-Histidine HCl 20.000

L-Isoleucine 50.000

L-Leucine 100.000

L-Lysine HCl 50.000

L-Methionine 20.000

L-Phenylalanine 50.000

L-Threonine 100.000

L-Tryptophan 50.000

Uracil 20.000

L-Tyrosine 50.000

L-Valine 140.000

Total 790.000

Total 27495.844

CSMComplete Supplement Mixture

DOBDrop Out Base

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As shown in the table below, synthetic defi ned media can be ordered in a number of ways:

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Yeast Media

Synthetic Defi ned Yeast Media includes all necessary components (page 11) for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers Synthetic Defi ned Media (SD) and Synthetic Defi ned Media with Agar (SDA) in avariety of formats for fl exibility and convenience.

Synthetic Defi ned Media (SD) can be ordered in a number of ways:

• A complete SD or SDA• A Drop Out Base (DOB) or Drop Out Base with Agar (DOBA) plus the appropriate amino acid supplement mixture (CSM)• A Yeast Nitrogen Base (YNB), a carbon source, the appropriate amino acid supplement mixture (CSM), and agar if desired• All raw materials separately

Synthetic Defi ned Media (SD)

Single Dropout FormulasPouches


SD Medium-LEU10x0.5 L 114811065

10x1 L 114811075

SD Medium-TRP 10x0.5 L 114812065

SD Medium-URA10x0.5 L 114813065

10x1 L 114813075

Double Dropout FormulasPouches


SD Medium-LEU-TRP 10x0.5 L 114823065

Triple Dropout FormulasPouches


SD Medium-HIS-LEU-TRP 10x0.5 L 114830065

4+ Drop Out FormulasPouches


SD Medium-ADE-HIS-LEU-TRP

10x0.5 L 114842065

Synthetic Defi ned Media Agar (SDA)

Single Dropout FormulasPouches


SDA Medium-LEU 10x0.5 L 114811165

SDA Medium-URA 10x0.5 L 114813165

Triple Dropout FormulasPouches


SDA Medium-HIS-LEU-TRP 10x0.5 L 114830165

SDA Medium-LEU-TRP-URA 10x1 L 114833175

Synthetic Defi ned Yeast Media

Ordering your selective media as an SD or SDA is the easiest option. Each SD or SDA formulation contains the appropriate CSM-based amino acid mixture and 1.7 g/liter YNB, 5 g/liter Ammonium Sulfate, and 20 g/liter Dextrose. SDA formulations also contain 17 g/L Agar.

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Yeast MediaDrop Out Base (DOB) and Drop Out

Base with Agar (DOBA)

DOB MediumUse 26.7 g/L.

Contents/liter: 1.7 g YNB, 5 g ammonium sulfate, 20 g dextrose

Size Cat. No.

Powder

227 g (0.5 lb) 114025012

454 g (1 lb) 114025022

1 kg (2.2 lb) 114025032

5 kg (11 lb) 114025042

DOB 2% Galactose (glucose-free)Contents/liter: 1.7 g YNB, 5 g ammonium sulfate, 20 g galactose

Size Cat. No.

Powder

454 g (1 lb) 114025522

DOB 2% Galactose, 1% Raffi noseUse 36.7 g/L. Contents/liter: 1.7 g YNB, 5 g ammonium sul-

fate, 20 g galactose, 10 g raffi nose

Size Cat. No.

Powder

227 g (0.5 lb) 114025912

DOBA MediumUse 43.7 g/L. Contents/liter: DOB, 17 g Agar-Y

Size Cat. No.

Powder

454 g (1 lb) 114026022

1 kg (2.2 lb) 114026032

5 kg (11 lb) 114026042

DOBA 2% Galactose (glucose-free)Use 43.7 g/L.

Contents/liter: DOB, 2% galactose, 17 g Agar-Y

Size Cat. No.

Powder

454 g (1 lb) 114026522

If you do not want to purchase a pre-made SD or SDA medium, or the combination of amino acids you need is not available, you can easily create your own SD or SDA by combining a DOB or DOBA with the appropriate amino acid mixture (page 11).

Yeast Nitrogen Base (YNB) Formulations

YNB w/o Ammonium SulfateUse 1.7 g/L

Size Cat. No.

Powder

227 g (0.5 lb) 114027012

454 g (1 lb) 114027022

YNB w/ Ammonium SulfateUse 6.7 g/L

Contents/liter: 1.7 g YNB, 5 g ammonium sulfate

Size Cat. No.

Powder

227 g (0.5 lb) 114027512

454 g (1 lb) 114027522

1 kg (2.2 lb) 114027532

5 kg (11 lb) 114027542

YNB VariationsDescription Size Cat. No.

YNB w/o Ammonium Sulfate, w/o Cop-per Sulfate,w/o Ferric Chloride

227 g (0.5 lb)

114027112

YNB w/Ammonium Sulfate, w/o Inositol (Powder)

227 g (0.5 lb)

114027412

YNB w/Ammonium Sulfate, w/o Phosphates, w/o Sodium Chloride

227 g (0.5 lb)

114027812

YNB w/o Ammonium Sulfate, w/o Folic Acid, w/o Ribofl avin

227 g (0.5 lb)

114030512

YNB w/Ammonium Sulfate, w/o thiamine HCl

227 g (0.5 lb)

114030712

To make your own synthetic defi ned yeast media, start with the YNB of your choice, add a carbon source, the appropriate amino acids, and agar if necessary.

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Complete Supplement Mixture (CSM)Description Size Cat. No.

Triple Dropout Formulations

CSM-HIS-LEU-TRP10 g 114530112

100 g 114530122

CSM-HIS-TRP-URA 10 g 114530812

CSM-LEU-TRP-URA 10 g 114530912

CSM-HIS-LEU-URA10 g 114531212

100 g 114531222

4+ Dropout Formulations

CSM-HIS-LEU-TRP-URA10 g 114540012

100 g 114540022

CSM-ADE-HIS-LEU-TRP 10 g 114540412

CSM-ADE-HIS-LEU-TRP-URA 100 g 114550122

CSM-HIS-LEU-MET-TRP-URA 10 g 114550412

CSM-ADE-HIS-LEU-MET-TRP-URA

100 g 114560222

Yeast Media

Complete Supplement Mixture (CSM)

The formulation for CSM has been tested as a dropout supplement for virtually all strains of S. cerevisiae.

Complete Supplement Mixture (CSM)Description Size Cat. No.

Complete Formulations

CSM10 g 114500012

100 g 114500022

Single Dropout Formulations

CSM-ADE 10 g 114510012

CSM-ARG 10 g 114510112

CSM-HIS10 g 114510312

100 g 114510322

CSM-LEU10 g 114510512

100 g 114510522

CSM-LYS 10 g 114510612

CSM-MET 10 g 114510712

CSM-TRP10 g 114511012

100 g 114511022

CSM-URA 10 g 114511212

CSM-URA 100 g 114511222

Double Dropout Formulations

CSM-LEU-TRP10 g 114520012

100 g 114520022

CSM-HIS-TRP 10 g 114520112

CSM-LEU-URA10 g 114520212

100 g 114520222

CSM-HIS-URA10 g 114520312

100 g 114520322

CSM-HIS-LEU10 g 114520412

100 g 114520422

CSM-TRP-URA10 g 114520512

100 g 114520522

CSM-MET-URA 10 g 114520812

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Yeast Media

Roll & Grow® Plating Beads

Safely and easily spread yeast and bacterial cells

• Spread cells in just seconds per plate

• Eliminate hazards of fl ame and ethanol

• No need for fragile glass “hockey sticks”

• Consistent, even colony distribution

• Sterile and easy to use

Roll & Grow® Plating Beads are used to spread 25-400 µL of bacterial or yeast cells across the surface of an agar plate, providing a safer, faster alternative to the traditional method of plating with a glass rod sterilized with ethanol and fl ame.

Sterile Roll & Grow® Plating Beads are provided in two skirted 50 mL conical tubes. Between six and seven 4 mm glass beads are used per plate for a total of ap-proximately 160 standard 100 mm plates.

Roll & Grow® Plating BeadsSize Cat. No.

2 tubes 115000550

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CELLect™ Bovine AlbuminsChromatographic Isolation for Optimal Performance

Greatest Biosafety• Sourced and purified in New Zealand• Negligible BSE risk• Absence of microbial agents

High Quality• ISO9001 and cGMP • Consistent product quality• Complete traceability

Best Performance• Enhanced performance compared to other isolation methods• Native structure and functionality retained

MP Bio CELLectTM BSA outperforms other albumin preparations

in mammalian cell culture (CHO and SP2/0)

Note: Cells were seeded in serum-free media, supplemented with various albumins in multiwell plates,with MTT analysis performed at day 3.

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Yeast Media

Growth Media for Schizosaccharomyces pombe

YES Broth (Vegetative Growth)Use 35 g/L

Contents/liter: 5 g YE, 30 g dextrose, 1x supplements (50 mg each ADE, HIS, LEU, LYS,URA)

Size Cat. No.

Powder

1 kg (2.2 lb) 114101532

YES Agar (Vegetative Growth)Use 52 g/L

Contents/liter: YES, 17 g agar

Size Cat. No.

Powder

1 kg (2.2 lb) 114101732

EMM Broth Use 32.2 g/L

Size Cat. No.

Powder

227 g (0.5 lb) 114110012

454 g (1 lb) 114110022

1 kg (2.2 lb) 114110032

Pouches

10 x 1 L 114110075

EMM w/o DextroseUse 12.35 g/L

Size Cat. No.

Powder

227 g (0.5 lb) 114110112

1 kg (2.2 lb) 114110132

EMM w/o Nitrogen Use 27 g/L

Contents/liter: EMM minus NH4Cl, glutamate

Size Cat. No.

Powder

227 g (0.5 lb) 114110712

Amino Acid Mixtures (HSM, BSM, SC)

BSM Dropout FormulationSize Cat. No.

BSM-HIS-LEU-TRP-URA

25 g 114740012

SC Complete FormulationSize Cat. No.

SC AMINO ACID MIXTURE

30 g 114400022

SC Dropout FormulationsSize Cat. No.

SC-HIS

30 g 114410222

SC-HIS-LEU-TRP-URA

30 g 114440022

SC-LEU

30 g 114410322

SC-TRP

30 g 114410522

SC-URA

30 g 114410622

SC-LEU-TRP

30 g 114420622

For full formulations visit our product pages at

www.mpbio.com

17


Joklik’s Modifi cation of Minimum Essential Medium (JMEM), Powder

Cat. No. Dextrose L-Glutamine Sodium Pyruvate

SodiumBicarbonate Phenol Red Other

Present (+), Absent (-) Size

091032322 (+) Penicillin(+) Streptomycin

1 x 10 L

Dulbecco’s Modifi ed Eagle’s Medium contains four times greater concentration of amino acids, vitamins and supple-mentary components than Eagle’s Basal Medium.

DMEM, 1X Liquid091233254 500 mL

091233354 500 mL

091642149 (-) Leucine 100 mL

091642254 (-) Methionine 500 mL

091642354 (-) Phosphate 500 mL

091642449 (-) Methionine,(-) Cystine,(-) Cysteine

100 mL

091642454 (-) Inositol 500 mL

091642954 500 mL

DMEM, Powder091033120 10 x 1 L

091033122 1 x 10 L

Ham’s F-10 and F-12 media are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham’s F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells.

F-10 Nutrient Medium, Liquid091240449 (+) 20 mM HEPES 100 mL

091440049 100 mL

F-10 Nutrient Medium (Ham’s F-10), Powder091040122 1 x 10 L

F-12 Nutrient Medium, Liquid091242354 500 mL

F-12 Nutrient Medium, Powder091042122 1 x 10 L

This medium is a 1:1 mixture of DMEM and Ham’s F-12 and specially suited for culturing cells without serum supple-mentation. It is one of the most universal media for serum-free cell culture techniques.

DMEM/F12 Nutrient Medium, Powder091047122 1 x 10 L

091046822 1 x 10 L

BME, Liquid

091600449BME 100X (+) Vitamins

100 mL

Mammalian Cell Culture Media

Ye

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Ce

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Classical media in powdered and liquid formatsfor mammalian cell culture applications

18

www.mpbio.com/media

Modifi ed Eagle’s Medium incorporates specifi c modifi cations such as higher amino acid concentrations for the cultivation of fastidious cells. MP’s MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation.

MEM, Liquid

Cat. No. Dextrose L-Glutamine Sodium Pyruvate

SodiumBicarbonate Phenol Red Other

Present (+), Absent (-) Size

091210354 500 mL

091210254 500 mL

091210254X6 6x500 mL

091622049 (+) Earle’s Salts(-) Arginine

100 mL

091641454

(+) Earle’s Salts(-) Methionine,(-) Cystine,(-) Cysteine

500 mL

091213454 (+) Hank’s Salts,20 mM HEPES

500 mL

091213254 (+) Hanks’ Salts 500 mL

091410054 MEM 10X (+) Earle’s Salts

500 mL

091601149 MEM 50X 100 mL

MEM, Powder091010120

(+) Earle’s Salts10x1 L

091010122 1x10 L

091031120 (-) Deoxyribosides(-) Ribosides

10x1 L091031122 1x10 L

RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes and is now a popular general purpose medium when properly supplemented.

McCoy’s, 5A Medium

091255354 Iwakata and Grace Modifi cation

500 mL

RPMI, Liquid091260349 100 mL

091260354 500 mL

091260454 (+) 20 mM HEPES 500 mL

091646754 500 mL

091629754 (-) Phosphate 500 mL

091629149 (-) Leucine 100 mL

091260254 500 mL

091646454 (-) Methionine,(-) Cystine,(-) Cysteine

500 mL

091646854 500 mL

RPMI, Powder091060120 10 x 1 L

091060122 1 x 10 L

091060124 1 x 50 L

William’s Medium E, Powder091050122 1 x 10 L

Mammalian Cell Culture Media

19


Murashige and Skoog Medium is based on the original 1962 formulation. Vitamins, macro-and micro-nutrients and complete media formulations are available inconvenient pouches of pre-mixed powder and in liquid.

Murashige & Skoog Macronutrients (4.232720 g/L)

Ammonium Nitrate 1.650000

Calcium Chloride Anhydrous 0.332020

Magnesium Sulfate Anhydrous 0.180700

Potassium Nitrate 1.900000


Murashige & Skoog Micronutrients (0.097890 g/L)

Boric Acid 0.006200

Cobalt Chloride-6H2O 0.000025

Cupric Sulfate Anhydrous 0.000025

Ferrous Sulfate-7H2O 0.027800

Manganese Sulfate-H2O 0.016900

Molybdic Acid Sodium Salt-2H2O 0.000250

Na2-EDTA-2H2O 0.037260


Zinc Sulfate-7H2O 0.008600

Murashige & Skoog Vitamins (0.103100 g/L)

Glycine 0.002000

myo-Inositol 0.100000

Nicotinic acid 0.000500

Pyridoxine-HCl 0.000500

Thiamine-HCl 0.000100

Murashige & Skoog Complete Medium w/Agar (g/L)

Sucrose 30

Agarose 8

Murashige & SkoogDescription Size Cat. No.

Basal Salt Mixture

macro- and micronutrients10 x 1 L 092623020

1 x 10 L 092623022

Macronutrients Stock Solution (10x)

macronutrients 500 mL 092624654

Medium

without sucrose, IAA,kinetin or agar

10 x 1 L 092610020

1 x 10 L 092610022

1 x 50 L 092610024

Plant Salt Mixture

without agar1 x 10 L 092633022

1 x 50 L 092633024

Vitamin Mixture (1000x) Powder

use at a concentration of1 mL/L medium

100 mL 092624849

Modifi ed Vitamin Solution (1000x)

thiamine: 1 g/L use at a concentration of 1 mL/L medium

100 mL 092625149

Basal Medium

macronutrients, micronutri-ents and vitamins

10 x 1 L 092623120

1 x 10 L 092623122

Basal Medium With Gamborg’s Vitamins

macro- and micronutrients and Gamborg vitamins

10 x 1 L 092623220

1 x 10 L 092623222

Plant Media

Pla

nt

Pla

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Murashige & Skoog Media Formulations

Find more culture growth media atwww.mpbio.com/media

20

www.mpbio.com/media

Gamborg’s B5 Media FormulationsGamborg’s B5 Medium is based on the original 1968 formulation. Complete Gamborg’s B5 media is ready-to-use and available in both powder and liquid form. A complete formulation with agar is also available as a powder. Gamborg’s B5 Basal Salts and Vitamin Mix are available separately as liquids and powders.

Gamborg’s B5 Macronutrients (2.86933 g/L)





Gamborg’s B5 Micronutrients (0.081150 g/L)

Boric Acid 0.003000

Cobalt Chloride-6H2O 0.000025

Cupric Sulfate Anhydrous 0.000025



Molybdic Acid Sodium Salt-2H2O 0.000250

Na2-EDTA-2H2O 0.037300



Gamborg’s B5 Vitamins (0.112000 g/L)

myo-Inositol 0.100000




Gamborg’s Complete Medium w/Agar

Sucrose 20

Agar 8

Gamborg’s B5Description Size Cat. No.

Basal Salt Mixture

macro- and micronutrients 1 x 10 L 092621622

Basal Salt Medium With Vitamins

macronutrients, micronutrientsand vitamins

1 x 10 L 092621522

Complete Agar

salts, vitamins, sucrose, agar 1 L Pouch 115101225

Chu’s N6 Media FormulationsChu’s N6 media is based on the original 1975 formulation. Complete Chu’s N6 media is ready-to-use and available in both powder and liquid form. A complete formulation with agar is also available as a powder. Chu’s N6 Basal Salts and Vitamin Mix are available separately as liquids and powders.

Chu’s N6 Macronutrients (3.908700 g/L)






Chu’s N6 Micronutrients (0.072330 g/L)

Boric Acid 0.001600



Na2-EDTA-2H2O 0.037250



Chu’s N6 Vitamins (0.004 g/L)

Glycine 0.002000




Chu’s N6 Complete Medium w/Agar (g/L)

Sucrose 20

Agar 8

Chu’s N6Description Size Cat. No.

Basal Salt Medium With Vitamins

macronutrients, micronutrientsand vitamins

1 x 10 L 092620522

Complete Agar

salts, vitamins, sucrose, agar 1 L Pouch 115102225

Plant Media

21


Plant Media HL5 Medium

HL5 MediumDescription Size Cat. No.

powder454 g (1 lb)

113090222

Specialty Media & Supplements

Hoagland’s Modifi ed Basal Salt MixtureDescription Size Cat. No.

macro- and micronutrients10 x 1 L 092621820

1 x 10 L 092621822

PlantCon™ Culture Containers

• Engineered for exceptional light transmission and optimal growth

• Sterilized by ethylene oxide

• Convenient and stackable to save on space

PlantCon™ Culture ContainersDescription Size Cat. No.

PlantCon™ Container, sterile200-sets(20 x 10)

092672206

PlantCon™ Base, sterile 10-pack 092672002

PlantCon™ Cover, sterile 10-pack 092672102

Cover dimensions: 9.5 x 9.5 x 7.0 cmBottom dimensions: 9.5 x 9.5 x 3.5 cm

FastPrep® Systems & KitsThe Most Advanced, Rapid and Thorough

Extraction of DNA, RNA and Proteins

• Homogenize resistant samples, fast and easy• Widest range of adapters for sample fl exibility• Eliminate cross contamination• Complete purifi cation with FastPrep kits P

lan

tP

lan

t

and Thoroughnd Proteins

22

www.mpbio.com/media

Carbon Sources/Sugars


D-Galactose, glucose-free (0.1% glucose)227 g (0.5 lb) 114008012

1 kg (2.2 lb) 114008032

D(+) Raffi nose pentahydrate100 g 114061522

454 g (1 lb) 114010022

1 kg (2.2 lb) 114010032

5-Fluoroorotic Acid

5-FOA 1 g 114066102

Glycerol, 80% (Sterile Solution)

80% Glycerol solution100 mL 113055034

500 mL 113055044

Hanks’ Balanced Salts (1X HBS Liquid, Modifi ed)

Without calcium and magnesium 500 mL 091810454

Without calcium, magnesium and phenol red 500 mL 091810554

HEPES Buffer

1M solution 100 mL 091688449

M9 Minimal Medium Salts

Powder 450 g 113035022

M9 Minimal Medium Salts (1-part Maniatic formulation)

Powder Contents/L of 5x solution: 33.9 g Na2HPO4, 15 g KH2PO4, 2.5 g NaCl, 5 g NH4Cl

227 g (0.5 lb) 113037012

1 kg (2.2 lb) 113037032

Nutrient Agar Stabs

Stabs are a convenient way to store or transport bacterial strains. Vials of soft nutrient agar are stabbed with a loop of a fresh culture and incubated overnight.

Contents/liter: Proprietary LB-like formulation containing glucose, cysteine and thymine.

50 stabs 113050033

Peptone-Y (Powder)

Casein peptone optimized for yeast media formulations. 1 kg (2.2 lb) 114018532

Phosphate Buffered Saline (PBS Powder)

Dulbecco’s Formula without calcium and magnesium10 x 1 L 091760420

1 x 10 L 091760422

Phosphate Buffered Saline (1X PBS Liquid)

Supplementary Products

23


7X™ Ready-to-Use Formulation

Cleaning labware has never been so easy!

Researchers have trusted 7X™ Detergents to clean labware for years. Now 7X™ is ready to use right from the bottle — just pump and go!

Available in a 1-gallon bottle with hand pump dispenser.

Find more information at mpbio.com/7X

eo

va

ReNo

Av

Supplementary Products


Dulbecco’s Formula without calcium and magnesium

100 mL 091860449

500 mL 091860454

6 x 500 mL 091860454X6

Phosphate Buffered Saline (10X PBS Liquid)

Dulbecco’s Formula without calcium and magnesium 500 mL 091960454

1X Trypsin-EDTA (1:250) in HBSS

0.25% (w/v) solution 100 mL 091689649

Tryptic Soy Agar

Used for the isolation and cultivation of a broad range of fastidious and non-fastidious microorganisms. Also, it may be used for sterility testing. Can be used with or without blood supplement.

Use 59.4 g/L.Contents/liter: 20 g polipeptone, 5 g sodium chloride, 10 g lactose, 10 g sucrose, 1 g dextrose, 0.2 g ferric ammonium sulfate, 0.2 g sodium thiosulfate, 0.025 g phenol red, 13 g agar.

500 g 091010617

Tryptic Soy Broth

Suited for the cultivation of a broad range of fastidious and non-fastidious microorganisms. It may also be used for the rapid determination of the bacteriological quality of water.

Use 30 g/L.Contents/liter: 17g casein peptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g dipotassium phosphate, 2.5 g dextrose

500 g 091010717

Tryptone-B

Tryptone-B is a dried and powdered pancreatic digest of casein. 500 g 091010817

One Source for All Your Sample Preparation

MP Biomedicals SARL

Parc d’Innovation, BP 5006767402 Illkirch CedexFrance

Tel: 33 03 8867 5425Toll Free: 800 7777 9999Fax: 33 03 8867 1945Email: [email protected]

For other offices andmore information,

please visit www.mpbio.com

MP Biomedicals LLC, HQ

3 Hutton Center DriveSuite 100, Santa AnaCalifornia 92707

Tel: 1 949 833 2500Toll Free: 1 800 854 0530Fax: 1 949 859 5010Email: [email protected]

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2 Pioneer PlaceSingapore 627885

Tel : 65 6775 0008Fax : 65 6774 6146

Email: [email protected]

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