AP Biology

Topic : Cell Membrane, its Structure, Function & Mechanisms Submitted To : Dr.Feroza

Wattoo Submitted By :

Victor Tabassum (11-arid-979) Asma Butool (11-arid-980) Mujahid Hussain (11-arid-981) Nauman Wahid (11-arid-982)

=>DVM 1st Semester<=

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AP Biology

The Cell Membrane (Introduction)

AP Biology

Overview Cell membrane separates living cell from

nonliving surroundings thin barrier = 8nm thick

Controls traffic in & out of the cell selectively permeable allows some substances to cross more easily

than others hydrophobic vs hydrophilic

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Plasma membrane of lipid bilayer lined from both sides by globular protein

Davson & Danielle proposed sandwich model

In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer

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Structure Of The Cell MembraneStructure Of The Cell Membrane

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Overview Made of phospholipids, proteins &

other macromolecules

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Phospholipids Fatty acid tails

hydrophobic

Phosphate group head hydrophilic

Arranged as a bilayer

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AP Biology

Phospholipid Bilayer

polarhydrophilic

heads

nonpolarhydrophobic

tails

polarhydrophilic

heads

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AP Biology

Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer

Extracellular fluid

Cholesterol

Cytoplasm

Glycolipid

Transmembraneproteins

Filaments ofcytoskeleton

Peripheralprotein

Glycoprotein

Phospholipids

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AP Biology

Membrane Fat Composition Varies Fat composition affects flexibility

membrane must be fluid & flexible about as fluid as thick salad oil

% unsaturated fatty acids in phospholipids keep membrane less viscous cold-adapted organisms, like winter wheat

increase % in autumn

cholesterol in membrane

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AP Biology

Membrane Proteins Proteins determine membrane’s specific functions

cell membrane & organelle membranes each have unique collections of proteins

Membrane proteins: peripheral proteins

loosely bound to surface of membrane cell surface identity marker (antigens)

integral proteins penetrate lipid bilayer, usually across whole membrane transmembrane protein transport proteins

channels, permeases (pumps)11

AP Biology

Function Of Membrane Proteins & Carbohydrates

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Overview Transport Enzyme Location Receptors,Transport Signals From

Outide To Cytoplasm Site For The Attachment With

Cytoskeleton

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Many Functions Of Membrane Proteins

Outside

Plasmamembrane

InsideTransporter Cell surface

receptorEnzymeactivity

Cell surface identity marker

Attachment to thecytoskeleton

Cell adhesion14

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Membrane Carbohydrates Play a key role in cell-cell recognition

ability of a cell to distinguish one cell from another antigens

important in organ & tissue development

basis for rejection of foreign cells by immune system

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AP Biology

Types Of Transport Across Cell Membrane

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Diffusion 2nd Law Of Thermodynamics

governs biological systems universe tends towards disorder (entropy)

Diffusion movement from high low concentration

Diffusion movement from high low concentration

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Diffusion Move from HIGH to LOW concentration

“passive transport” no energy needed

diffusion osmosis

movement of water

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AP Biology

Diffusion Across Cell Membrane Cell membrane is the boundary between

inside & outside… separates cell from its environment

INfoodcarbohydratessugars, proteinsamino acidslipidssalts, O2, H2O

OUTwasteammoniasaltsCO2

H2O products

cell needs materials in & products or waste out

IN

OUT

Can it be an impenetrable boundary? NO!

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AP Biology

Diffusion Through Phospholipid Bilayer

What molecules can get through directly? fats & other lipids

inside cell

outside cell

lipid salt

aa H2Osugar

NH3

What molecules can NOT get through directly?

polar molecules H2O

ions salts, ammonia

large molecules starches, proteins

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AP Biology

What Determines The Rate Of Diffusion?

1. The steepness of the concentration gradient: The bigger the difference between the two sides of the membrane the quicker the rate of diffusion.

2. Temperature: Higher temperatures give molecules or ions more kinetic energy. Molecules move around faster, so diffusion is faster.

3. The surface area: The greater the surface area the faster the diffusion can take place. This is because the more molecules or ions can cross the membrane at any one moment.

4. The type of molecule or ion diffusing: Large molecules need more energy to get them to move so they tend to diffuse more slowly. Non-polar molecules diffuse more easily than polar molecules because they are soluble in the non polar phospholipid tails.

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Molecules That Diffuse Through Cell Membranes1. Oxygen – Non-

polar so diffuses very quickly.

2. Carbon dioxide – Polar but very small so diffuses quickly.

3. Water – Polar but also very small so diffuses quickly.

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AP Biology

Channels Through Cell Membrane Membrane becomes semi-permeable

with protein channels specific channels allow specific material

across cell membrane

inside cell

outside cell

sugaraaH2O

saltNH3 23

AP Biology

Facilitated Diffusion Diffusion through protein channels

channels move specific molecules across cell membrane

no energy needed

“The Bouncer”“The Bouncer”

open channel = fast transport

facilitated = with help

high

low24

AP Biology

Active Transport

“The Doorman”“The Doorman”

conformational change

Cells may need to move molecules against concentration gradient shape change transports solute from

one side of membrane to other protein “pump” “costs” energy = ATP

ATP

low

high25

AP Biologysymportantiport

Active Transport Many models & mechanisms

ATP ATP

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AP Biology

Getting Through Cell Membrane Passive Transport

Simple diffusion diffusion of nonpolar, hydrophobic molecules

lipids high low concentration gradient

Facilitated transport diffusion of polar, hydrophilic molecules through a protein channel

high low concentration gradient

Active transport diffusion against concentration gradient

low high uses a protein pump requires ATP

ATP

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Transport Summary

simplediffusion

facilitateddiffusion

activetransport

ATP

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How About Large Molecules? Moving large molecules into & out of cell

through vesicles & vacuoles endocytosis

phagocytosis = “cellular eating” pinocytosis = “cellular drinking”

exocytosis

exocytosis29

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Endocytosis

phagocytosis

pinocytosis

receptor-mediated endocytosis

fuse with lysosome for digestion

non-specificprocess

triggered bymolecular signal

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The Special Case Of Water

Movement Of Water Across The Cell Membrane (OSMOSIS)

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Osmosis Is Diffusion Of Water Water is very important to life,

so we talk about water separately Diffusion of water from

high concentration of water to low concentration of water across a

semi-permeable membrane

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AP Biology

Concentration Of Water Direction of osmosis is determined by

comparing total solute concentrations Hypertonic - more solute, less water

Hypotonic - less solute, more water

Isotonic - equal solute, equal water

hypotonic hypertonic

water

net movement of water33

AP Biology freshwater balanced saltwater

Managing Water Balance Cell survival depends on balancing

water uptake & loss

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Managing Water Balance Isotonic

animal cell immersed in mild salt solution example:

blood cells in blood plasma problem: none

no net movement of water flows across membrane

equally, in both directions

volume of cell is stable

balanced35

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Managing Water Balance Hypotonic

a cell in fresh water example: Paramecium problem: gains water,

swells & can burst water continually enters

Paramecium cell

solution: contractile vacuole pumps water out of cell ATP

plant cells turgid

freshwater

ATP

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Managing Water Balance Hypertonic

a cell in salt water example: shellfish problem: lose water & die solution: take up water or

pump out salt plant cells

plasmolysis = wilt

saltwater37

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Cell (compared to beaker) hypertonic or hypotonic

Beaker (compared to cell) hypertonic or hypotonic

Which way does the water flow? in or out of cell

.05 M .03 M

Osmosis…

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