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Chapter 10 Gene Isolation and Manipulation
Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.
Finding specific nucleic acids by using gel electrophoresis and
blotting
Polymerase chain reaction (PCR)
Sequencing
The structure of 2’,3’-dideoxynucleotides
Sanger Sequencing (Dideoxy Sequencing)
The dideoxy sequencing method
Reading the DNA sequence from an automatic sequencer
How to amplify an interesting gene
Making a cDNA clone
Introduction of a transgene
Agrobacterium tumefaciens
causal agent of Crown Gall
The Ti plasmid vector
The generation of a transgenic plant
The generation of a transgenic plant
Pattern of transmission of T-DNA