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CitrusCyc: Metabolic Pathway Databases for the C. clementina and C. sinensis
Genomes
Surya Saha1, Mirella Flores-Gonzalez1, Jodi L Humann2, Dorrie
Main2 and Lukas Mueller1
1Boyce Thompson Institute; 2Washington State University
[email protected] @SahaSurya
PAG XXV
Citrus Genome Workshop
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Acknowledgements
Mueller Lab:
Mirella Flores
Prashant Hosmani
Main Lab:
Jodi Humann
Sook Jung
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Citrus Genomics Resources
C. clementina genome
C. sinensis genome
C. trifoliata genome
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Citrus Reference Genomes
• C. clementina (Clementine mandarin) v 1.0• 301.4 Mb with 1,398 scaffolds• 9 chromosomal pseudo-molecules (~21-51 Mb)• 24,533 proteins• 33,929 primary and alternate transcripts
• C. sinensis (Sweet orange) v 1.1• 319 Mb with 12,574 scaffolds• 236 scaffolds > 251 kb but no pseudomolecules• 25,376 proteins• 46,147 primary and alternate transcripts
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Pathway Tools ConceptsPathway/Genome Database (PGDB) – combines information about
• Pathways, reactions, substrates
• Enzymes, transporters
• Genes, replicons
• Transcription factors/sites, promoters,
operons
Tier 1: Literature-Derived PGDBs• MetaCyc, HumanCyc, YeastCyc
• EcoCyc -- Escherichia coli K-12
• AraCyc – Arabidopsis thaliana
Tier 2: Computationally-derived DBs, Some Curation -- 34 PGDBs
• Bacillus subtilis, Mycobacterium tuberculosis
Tier 3: Computationally-derived DBs, No Curation -- The remainderSource: Peter Karp (SRI)
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Pathway Tools ConceptsMetaCyc
2491 pathways from 2816 different organisms
Pathways involved in • Both primary and secondary metabolism• Associated metabolites, reactions, enzymes, and genes.
Enzyme Commission numbers (1.1.1.1)
• Numerical classification scheme for enzymes, based on the chemical reactions they catalyze.
• Recommended name
• Catalytic activity
• Cofactors (if any)
• Pointers to the Swiss-Prot protein sequence entrie(s) (if any)
7,000+ databases from many institutions world-wide. 5,000+ on BioCyc.org
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Pathway/Genome Database Construction
Source: Peter Karp (SRI)
Genes Proteins Regulation Reactions Pathways
Enzyme Commission numbers from Uniprot
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Pathway/Genome Database Construction
Annotated Genome
e.g. C sinensis
PathoLogic
Software
Reference Pathway
Database (MetaCyc)
Reactions
Pathways
Compounds
Gene
productsGenes
Pathway/Genome
Database (CitrusCyc)
Source: Peter Karp (SRI)
Predicts metabolic pathways
Predicts which genes code for missing enzymes in metabolic pathways
Infers transport reactions from transporter names
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Pathway Tools Software
Pathway/Genome
Editors
Pathway/Genome
Database
PathoLogic
Platform
Annotated
Genome
Pathway/Genome
Navigator
Briefings in Bioinformatics 11:40-79 2010
Genome-Scale
Flux Model
Source: Peter Karp (SRI)
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CitrusCyc
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
• 25,376 proteins• 46,147 primary and alternate
transcripts• 38,964 transcripts have EC
numbers• 1217 unique EC numbers
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C clementina Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CCLEMENTINA_ML
• 25,376 proteins• 46,147 primary and alternate
transcripts• 28,630 transcripts have EC
numbers• 1179 unique EC numbers
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
Cellular Overview
Secondary metabolite biosynthesis Membrane pathways
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
Photosynthesis pathway
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
Photosynthesis pathway
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
Photosynthesis pathway
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C sinensis Cyc v3.0http://pathways.citrusgenomedb.org/organism-summary?object=CSINENSIS_ML
Photosynthesis pathway
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Overlay of RNAseq Results
Cellular Overview of Diaphorina citriBased on NCBI v100
Membrane proteins
Secretory proteins
Membrane proteins
Kruse et al. (unpublished)
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Future Work
• Meta – CitrusCyc
• Manual curation of pathways
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P0369
Dorrie Main
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CitrusGreening.org
Host, Vector and Pathogen(s)
Blast Databases
BioCycs
JBrowse
FTP site
News
Publications
Social Media Twitter: 280 followersFacebook: 71 followers
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P0368: Systems Biology Resources for the Citrusgreening Disease Complex
Mirella Flores
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Thank you!!