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Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator Author(s): Pradella, JGD; Ienczak, JL ; Delgado, CR; Taciro, MK | E-mail: [email protected] Source: BIOTECHNOLOGY LETTERS Volume: 34 Issue: 6 Pages: 1003-1007 | DOI: 10.1007/s10529-012-0863-1 Published: JUN 2012 Abstract: Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l(-1)) was achieved using soybean oil at 40 g l(- 1) and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l(-1) h(-1), and the calculated Y-p/s value was 0.85 g g(-1). Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe. Full Text: http://www.springerlink.com/content/1r81n10176656441/?MUD=MP Addresses: [da Cruz Pradella, Jose Geraldo; Ienczak, Jaciane Lutz] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, BR-13083970 Campinas, SP, Brazil [Delgado, Cecilia Romero] Inst Pesquisas Tecnol Estado Sao Paulo SA, Lab Biotecnol Ind, BR-05508901 Sao Paulo, Brazil [Taciro, Marilda Keico] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, Brazil
