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Curriculum Vitae Dr. Anwar Ullah 2017
Curriculum Vitae
Dr. Anwar Ullah
2017
Figure on the cover: Left; crystal structure of 2S albumin from Moringa oleifera seeds (Ullah et al., BBRC, 2015) and right; crystal
structure of LAAO (Ullah et al., BBRC, 2012).
1. Personal Biodata
Full Name : Dr. Anwar Ullah
Gender : Male
Date of birth : 18/04/1983
Marital status : Married
Permanent residence : Brazil
Postal address : Department of Biosciences COMSATS Institute of Information Technology, Park Road 45550
Islamabad, Pakistan. Cell No. : +92-334-0111362
E-mail address : [email protected],
2. Academic Qualification:
Exam. Year of
Passing Division/Marks Obtained (%)
Board/University Subjects
S.S.C.
2000
1st
/A
637/850 (75%)
B. I.S.E. Peshawar
Science
F. Sc.
2002
1st
/A
833/1100 (76%)
B.I.S.E. Mardan
Biology, Chemistry, Physics
B.Sc. 2004 1st 423/550 (77%)
Univ. of Peshawar
Biology, Chemistry
M. Sc. 2006 1st 786/1200 (65.5)
Univ. of Peshawar
Chemistry (Biochemistry)
Ph. D.
2013 1st
A grade
State University of Sao Paulo- UNESP, Brazil.
Molecular Biophysics (Protein crystallography)
Post-doc. 2015 1st A grade
State University of Sao Paulo- UNESP, Brazil.
Molecular Biophysics (Protein crystallography)
Assistant Professor
2016 ------------ COMSATS Institute of Information Technology
Islamabad.
Chemistry and Biochemistry
Ph. D. thesis title: Purification and structural characterization of snake venom proteins.
3. Additional credits
1) 1st position in the school in Matric (10th Grade).
2) 1st position in F.Sc. in College.
3) 1st position in B. Sc. in College.
4) Qualified Graduate Assessment Test (GAT) six times.
5) Qualified GAT Subject test (Chemistry).
Fellowships awarded
1) Ph. D. CNPq-TWAS- Brazil, Italy (2009-2013).
2) Post-doc. FAPESP-Brazil (2013-2015).
3) Biovision fellowship, Egypt (2012).
4) ICTP, fellowship South Africa (2013).
Languages:
1) English-(Excellent)
2) Portuguese-(Excellent)
3) Urdu-(Excellent)
4) Pashto-(Excellent)
5) Spanish-(Good)
6) Arabic-(Good)
Expertise and research interests Biochemistry, Structural and Molecular Biology, Purification, characterization
and crystallization of proteins from both natural and recombinant sources,
Protein crystallography, structure determination, structure base drug discovery,
protein-protein and protein-ligand interaction, structure bioinformatics, crystal
diffraction, data collection, data processing, and structural solution.
4. List of Publications
A) Research articles
1) Spider's Venom Phospholipases D: A structural review.
Masood R, Ullah K, Ali H, Ali I, Betzel C, Ullah A.
Int. J. Biol. Macromol. 2017 Sep 23. pii: S0141-8130(17)31422-8.
doi: 10.1016/j.ijbiomac.2017.09.081.
Impact Factor: 3.67
2) Ac2-26 peptide and serine protease of Bothrops atrox similarly
induces angiogenesis without triggering local and systemic inflammation
in a murine model of dorsal skinfold chamber.
Molás RB, de Paula-Silva M, Masood R, Ullah A, Gimenes AD, Oliani SM.
Toxicon. 2017 Oct; 137:7-14. doi: 10.1016/j.toxicon.2017.06.009.
Impact Factor: 2.50
3) Potential Implications for Designing Drugs Against the Brown
Spider Venom Phospholipase-D.
Chaves-Moreira D, de Moraes FR, Caruso ÍP, Chaim OM, Senff-Ribeiro A,
Ullah A, da Silva LS, Chahine J, Arni RK, Veiga SS. J. Cell. Biochem. 2017
Apr 07. doi: 10.1002/jcb.25678. PMID: 27563734
Impact Factor: 3.25
4) Serine proteinases from Bothrops atrox and Bothrops brazili
facilitate angiogenesis upon inducing PI3K/Akt signaling axis.
Shreesha K Bhat, Manjunath B Joshi, Anwar Ullah, Rehana Masood, Setlur G
Biligiri, , Raghuvir K Arni, K. Satyamoorthy, Toxicon, Dec 1. 2016.124:63-72.
DOI: 10.1016/j.toxicon.2016.11.001. PMID: 27816537
Impact Factor: 2.50
5) Tyrosine binding and promiscuity in the arginine repressor from the
pathogenic bacterium Corynebacterium pseudotuberculosis.
Mariutti RB, Ullah A, Araujo GC, Murakami MT, Arni RK. Biochem. Biophys.
Res. Commun. 2016 Jul 8; 475 (4):350-5.
doi: 10.1016/j.bbrc.2016.05.091. PMID: 27233609
Impact Factor: 2.41
6) Functional Expression, Monodispersity and Conformational
Changes in the SBMV virus viral VPg on binding TFE.
Mariutti RB, Caruso IP, Ullah A, De Morais FR, Rehders D, Arni RK. Int. J. Biol.
Macromol. 2015 (15); 30118-5.
doi: 10.1016/j.ijbiomac.2015.11.026. PMID: 26592780
Impact Factor: 2.86
7) Crystal structure of mature 2S albumin from Moringa oleifera seeds.
Ullah A, Mariutti RB, Masood R, Caruso IP, Gravatim Costa GH, Millena de
Freita C, Santos CR, Zanphorlin LM, Rossini Mutton MJ, Murakami MT, Arni
RK. Biochem. Biophys. Res. Commun. 2015 (15) 30787-7.
doi: 10.1016/j.bbrc.2015.10.087. PMID: 26505799.
Impact Factor: 2.41
8) Crystal structure of Staphylococcus aureus exfoliative toxin D-like
protein: Structural basis for the high specificity of exfoliative toxins.
Mariutti RB, Souza TA, Ullah A, Caruso IP, de Moraes FR, Zanphorlin LM,
Tartaglia NR, Seyffert N, Azevedo VA, Le Loir Y, Murakami MT, Arni RK.
Biochem. Biophys. Res. Commun. 2015 467(1):171-7.
doi: 10.1016/j.bbrc.2015.08.083. PMID: 26299923.
Impact Factor: 2.41
9) Ac2-26 Mimetic Peptide of Annexin A1 Inhibits Local and Systemic
Inflammatory Processes Induced by Bothrops moojeni Venom and the
Lys-49 Phospholipase A2 in a Rat Model.
Stuqui B, de Paula-Silva M, Carlos CP, Ullah A, Arni RK, Gil CD, Oliani SM.
PLoS One. 2015; 10 (7):e0130803.
doi: 10.1371/journal.pone.0130803. PMID: 26147724.
Impact Factor: 3.32
10) Preparation and Characterization of Monomodal Grapevine Virus A
Capsid Protein.
Santana VS, Mariutti RB, Eberle RJ, Ullah A, Caruso IP, Arni RK. Protein
Pept. Lett. 2015; 22 (8):712-8.
doi: 10.2174/0929866522666150625111607. PMID: 26112976.
Impact Factor: 1.01
11) Structural Insights into Substrate Binding of Brown Spider Venom
Class II Phospholipases D.
Coronado MA, Ullah A, da Silva LS, Chaves-Moreira D, Vuitika L, Chaim OM,
Veiga SS, Chahine J, Murakami MT, Arni RK. Curr. Protein Pept. Sci. 2015;
16 (8):768-74. doi: 10.2174/1389203716666150505231625. PMID: 25961401.
Impact Factor: 3.15
12) Crystallization and preliminary X-ray diffraction studies of an L-
amino-acid oxidase from Lachesis muta venom.
Ullah A, Masood R, Spencer PJ, Murakami MT, Arni RK. Acta Crystallogr. F
Struct. Biol. Commun. 2014 70 (Pt.11):1556-9.
doi: 10.1107/S2053230X14017877. PMID: 25372830.
Impact Factor: 0.54
13) Crystallization and preliminary X-ray diffraction analysis of a novel
sphingomyelinase D from Loxosceles gaucho venom.
Ullah A, Magalhães GS, Masood R, Mariutti RB, Coronado MA, Murakami MT,
Barbaro KC, Arni RK. Acta Crystallogr. F Struct. Biol. Commun. 2014 70 (Pt
10):1418-20. doi: 10.1107/S2053230X14019207. PMID: 25286953.
Impact Factor: 0.54
14) Recent advances in the understanding of brown spider venoms:
From the biology of spiders to the molecular mechanisms of toxins.
Gremski LH, Trevisan-Silva D, Ferrer VP, Matsubara FH, Meissner GO, Wille
AC, Vuitika L, Dias-Lopes C, Ullah A, de Moraes FR, Chávez-Olórtegui C,
Barbaro KC, Murakami MT, Arni RK, Senff-Ribeiro A, Chaim OM, Veiga SS.
Toxicon. 2014 83: 91-120. doi: 10.1016/j.toxicon.2014.02.023. PMID:
24631373.
Impact Factor: 2.50
15) Rapid purification of serine proteinases from Bothrops alternatus
and Bothrops moojeni venoms.
Fernandes de Oliveira LM, Ullah A, Masood R, Zelanis A, Spencer PJ, Serrano
SM, Arni RK. Toxicon. 2013, 76: 282-90.
doi: 10.1016/j.toxicon.2013.10.016. PMID: 24140922.
Impact Factor: 2.50
16) Crystal structure of Jararacussin-I: the highly negatively charged
catalytic interface contributes to macromolecular selectivity in snake
venom thrombin-like enzymes.
Ullah A, Souza TA, Zanphorlin LM, Mariutti RB, Santana VS, Murakami MT,
Arni RK. Protein Sci. 2013 22(1):128-32.
doi: 10.1002/pro.2189. PMID: 23139169.
Impact Factor: 2.86
17) Purification, crystallization and preliminary X-ray diffraction
analysis of a class P-III metalloproteinase (BmMP-III) from the venom of
Bothrops moojeni.
Ullah A, de Souza Tde A, Masood R, Murakami MT, Arni RK. Acta
Crystallogr. Sect F Struct. Biol. Cryst .Commun. 2012 68 (Pt. 10):1222-5.
doi: 10.1107/S1744309112036603. PMID: 23027752.
Impact Factor: 0.54
18) Purification, crystallization and preliminary X-ray diffraction
analysis of crotamine, a myotoxic polypeptide from the Brazilian snake
Crotalus durissus terrificus.
Coronado MA, Georgieva D, Buck F, Gabdoulkhakov AH, Ullah A, Spencer PJ,
Arni RK, Betzel C. Acta Crystallogr. Sect. F Struct. Biol. Cryst .Commun.
2012 68 (Pt. 9):1052-4. doi: 10.1107/S1744309112032721. PMID: 22949192.
Impact Factor: 0.54
19) Crystallographic portrayal of different conformational states of a
Lys49 phospholipase A₂ homologue: insights into structural determinants
for myotoxicity and dimeric configuration.
Ullah A, Souza TA, Betzel C, Murakami MT, Arni RK. Int. J. Biol. Macromol.
2012 51(3):209-14. doi: 10.1016/j.ijbiomac.2012.05.006. PMID: 22584077.
Impact Factor: 2.60
20) Structural insights into selectivity and cofactor binding in snake
venom L-amino acid oxidases.
Ullah A, Souza TA, Abrego JR, Betzel C, Murakami MT, Arni RK. Biochem.
Biophys. Res. Commun. 2012 421(1):124-8.
doi: 10.1016/j.bbrc.2012.03.129. PMID: 22490662.
Impact Factor: 2.41
21) Crystallization and preliminary X-ray diffraction analysis of an L-
amino-acid oxidase from Bothrops jararacussu venom.
Ullah A, Coronado M, Murakami MT, Betzel C, Arni RK. Acta Crystallogr.
Sect. F Struct. Biol. Cryst. Commun. 2012 68 (Pt. 2): 211-3.
doi: 10.1107/S1744309111054923. PMID: 22298002.
Impact Factor: 0.54
22) Structure of a novel class II phospholipase D: catalytic cleft is
modified by a disulphide bridge.
de Giuseppe PO, Ullah A, Silva DT, Gremski LH, Wille AC, Chaves Moreira D,
Ribeiro AS, Chaim OM, Murakami MT, Veiga SS, Arni RK. Biochem. Biophys.
Res. Commun. 2011 409 (4):622-7.
doi: 10.1016/j.bbrc.2011.05.053. PMID: 21616057
Impact Factor: 2.41
23) Crystallization and preliminary X-ray diffraction analysis of a class
II phospholipase D from Loxosceles intermedia venom.
Ullah A, de Giuseppe PO, Murakami MT, Trevisan-Silva D, Wille AC, Chaves-
Moreira D, Gremski LH, da Silveira RB, Sennf-Ribeiro A, Chaim OM, Veiga SS,
Arni RK. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2011 67 (Pt.
2):234-6. doi: 10.1107/S1744309110050931. PMID:21301094.
Impact Factor: 0.54
24) Removal of Methylene Blue Dye from Aqueous Solution by Using
Powdered Saccharum Arundinaceum Leaves
Muhammad Ullah, Khakemin Khan, Rasool Kamal, Muhammad Sohail,
Abid Ullah, Kifayat Ullah, Anwar Ullah, Shabnum Shaheen, Inayat Ullah,
Zafar Ali Shah, Transylvanian Review, 2017 Vol XXV, No. 18. 4746-4752.
Impact Factor: 0.048
25) Glycosylation in Snake Venom Proteinases.
Monika Coronado, Raphael J Eberle, Anwar Ullah, Raghuvir K Arni.
Toxicon. Submitted, Jan 02, 2017.
Impact Factor: 2.50
B) Book chapters:
1) Structural Biology of Toxins; Three-dimensional Structures and
Mechanisms of Snake Venom Serine Proteinases, Metalloproteinases and
Phospholipase A2s.
M. A. Coronado, F. R. de Moraes, A. Ullah, R. Masood, V.S. Santana, R.
Mariutti, H. Brognaro, D.Georgieva, M.T. Murakami C. Betzel and R. K. Arni.
Toxinology, 2014, pp 1-25.
C) Citations
Goog. Scholar ID: https://scholar.google.com.br/citations?user=a7ntkpcAAAAJ&hl=en
D) Potential reviewer of the journals
1) Journal of Intercultural Ethnopharmacology
2) Journal of Pathophysiology
3) Eminent Association of Researchers in Biological & Medical Sciences
(EARBM)
4) Journal of Animal Research and Veterinary Science
5) Energies
E) Research/Scientific collaborations
1) Christian Betzel, Laboratory of Structural Biology of Infection, Institute of
Biochemistry and Molecular Biology, University of Hamburg, c/o DESY,
Notkestrasse 85, Building 22a, D-22603 Hamburg, Germany.
2) Prof. Dr. Terese Bergfors, Uppsala University, Sweden.
3) Prof. Dr. Pernille Harris, Department of Chemistry, DTU, Kemitorvet,
Building 207, DK-2800 Kgs. Lyngby, Denmark.
4) Prof. Dr. Mikako Shirouzu, Division of Structural and Synthetic Biology
RIKEN Center for Life Science Technologies Japan.
5) Prof. Dr. Mario Tyago Murakami, Brazilian National Synchrotron Lab.
Campinas- SP, Brazil.
6) Adelia Cristina Oleveira Cintra, Depto. de Análises Clinicas,
Toxicológica em outro s e Bromatológicas - DACTB Faculdade de
Ciências Farmacêuticas de Ribeirão Preto – FCFRP Universidade de São
Paulo – USP.
7) Katia Cristina Barbaro Nogueira and Geraldo Santana Magalhães,
Pesquisadores Cientificos Laboratório de Imunopatologia Instituto
Butantan Avenida Dr. Vital Brasil, 1500 Butantã-São Paulo-SP.
8) Patrick Jack Spencer, Centro de Biotecnologia, IPEN/CNEN, Av. Lineu
prestes 2242, Cidade Universitária 05508-000 São Paulo, SP.
9) Olga Meiri Chaim and Silvio Sanches Veiga, Departamento de Biologia
Celular, Universidade Federal do Parana, 81531-960 Curitiba-PR, Brazil.
5. Structures deposited to Protein Data Bank (PDB)
1) Crystal structure, maturation and flocculating properties of a 2S albumin
from Moringa oleifera seeds (PDB Code; 5DOM).
2) Molecular insights into the specificity of exfoliative toxins from
Staphylococcus aureus (PDB Code; 5C2Z).
3) Crystal structure of the Arginine Repressor from the pathogenic bacterium
Corynebacterium pseudotuberculosis (PDB Code; 5JVO).
4) Structure of L-amino acid oxidase (LAAO) from Bothrops jararacussu
venom (PDB Code; 4EOV).
5) Crystal structure of Loxosceles intermedia phospholipase D isoform 1
H12A mutant (PDB Code; 3RLG).
6) Structure of a novel class II phospholipase D: catalytic cleft is modified by
a disulphide bridge (PDB Code; 3RLH).
7) Structure of MTX-II from Bothrops brazili (PDB Code; 4DCF).
8) Crystal structure of Notexin at 1.8 Å resolution (PDB Code; 4E4C).
9) Crystal structure of Jararacussin-I. (PDB Code; 4GSO).
10) Structural insights into substrate binding of brown spider venom
class II phospholipases D. (PDB Code; 4RW3, 4RW5).
6. International conferences and courses attended
1) V Proteomics Workshop, Brazilian Biosciences National Laboratory
(LNBio), Brazilian Center for Research in Energy and Materials
(CNPEM) Campus, 5th - 6th November 2014, Campinas-SP, Brazil.
2) 1st UNESP NMR Symposium Recent Advances in NMR
Spectroscopy: A Powerful Tool for Fundamental and Applied
Research November 7th 2014, IQ-UNESP, Araraquara, SP, Brazil.
3) Advanced school on "Synchrotron Radiation Techniques and
nanotechnology: a synergic approach to the Life Sciences and
Medicine. 11-22 November 2013, iThemba LABS Cape Town,
South Africa.
4) 45th course Present and Future Methods for Biomolecular
crystallography, Erice, Italy, 31 May-10 June 2012.
5) 4th International conference on natural Toxin, Egypt 20-22
December 2010.
6) BioVision Nxt Alexandria Egypt, 21-15 April 2012.
7) Protein Purification Day 2012, GE Healthcare Life Sciences, 16
August 2012.
8) International Interdisciplinary Science Conference (I-ISC), 2012 on
Protein Folding and Diseases, New Dehli, India.
7. Poster and talks presented in the Conferences
1) Purification and structural characterization of proteins from snake
and spider venom, plant seeds and bacteria, International Nathiagali
Summer College (INSC) on Physics and Contemporary Needs-2017,
Islamabad, invited speaker.
2) The first crystal structure of a snake venom phospholipase B from B.
brazili venom, Poster, International Nathiagali Summer College (INSC)
on Physics and Contemporary Needs-2017.
3) Initial insights into the crystal structure of phospholipase B from
Bothrops brazili venom, EURASIA-2016, ICCBS-Karachi, Invited
speaker.
4) Purification, characterization and structural determination of a class I
metalloproteinase from Bothrops brzili venom.
Anwar Ullah; Rehana Masood ; Ricardo Barros Mariutti ; Mario Tyago
Murakami; Raguvir Krishnaswamey Arni, Pakistan society for
Biochemistry and Molecular Biology and Centre for advanced drug
Research, 25-27 August 2016, CIIT Abbottabad.
5) Expression, Purification and characterization of Thioredoxin
Reductase from Corynebacterium pseudotuberculosis.
Rehana Masood ; Anwar Ullah; Ricardo Barros Mariutti ; Raguvir
Krishnaswamey Arni, Pakistan society for Biochemistry and Molecular
Biology and Centre for advanced drug Research, 25-27 August 2016,
CIIT Abbottabad.
6) High-resolution crystal structures of bothrombin (Bothrops jararaca)
and jararacussin-II (Bothrops jararacussu)
Anwar ullah, Rehana Masood, Ricardo Barros Mariutti , Icaro Putinhon
Caruso, Mário Tyago Murakami, Raghuvir Krishnaswamy Arni. 23rd
international congress of the iubmb and 44th annual meeting of the
Brazilian society for biochemistry and molecular biology (sbbq) in
Foz do Iguaçu, Paraná, brazil, august 24th to 28th, 2015.
7) Expression, purification and crystal structure of triose phosphate
isomerase from Corynebacterium pseudotuberculosis, Rehana
Masood, Anwar ullah, Ricardo Barros Mariutti, Icaro Putinhon Caruso,
Mário Tyago Murakami, Raghuvir Krishnaswamy Arni. 23rd international
congress of the iubmb and 44th annual meeting of the Brazilian
society for biochemistry and molecular biology (sbbq) in foz do
Iguaçu, Paraná, brazil, august 24th to 28th, 2015.
8) Crystal structure of recombinant exfoliative toxin d from
staphylococcus aureus. Ricardo Barros Mariutti , Anwar ullah, Rehana
Masood, Icaro Putinhon Caruso, Mário Tyago Murakami, Raghuvir
Krishnaswamy Arni. 23rd international congress of the iubmb and 44th
annual meeting of the Brazilian society for biochemistry and
molecular biology (sbbq) in foz do Iguaçu, Paraná, brazil, august 24th
to 28th, 2015.
9) Purification, characterization and crystallization of
phosphodiesterases from the venom of Bothrops species.
Anwar Ullah, Rehana Masood, M. T. Murakami and R. K. Arni, Latin
American Summit Meeting on Biological Crystallography and
Complementary Methods, National Center for Research in Material
and Energy (CNPEM), Campinas-SP, Brazil, 22-24 September, 2014.
10) Catalytic mechanisms of sphingomyelinase D.
Ullah, A., M. A. Coronado and R. K. Arni. Enzymes mechanisms by
biological system (EMBO-conference), 1–4 June 2014, UK–Manchester.
11) Purification and structural characterization of snake venom proteins.
Anwar Ullah, Rehana Masood, M. T. Murakami and R. K. Arni. Advanced
school on Synchrotron Radiation Techniques and nanotechnology: a
synergic approach to the Life Sciences and Medicine. 11-22
November, 2013, iThemba LABS, Cape Town, South Africa.
12) Structural and computational studies of the catalytic mechanism of
sphingomyelinase d from loxosceles intermedia spider venom.
Anwar ullah; Luciane sussuchi da silva; Rehana Masood; Larissa vuitika;
Olga meiri chaim;Silvio sanches veiga; Jorge chahine; Raghuvir
krishnaswamy Arni. XI Congress of the Pan American Section of the
International Society on Toxinology and the XII Congress of the
Brazilian Society of Toxinology, page No. 168, 3-8 November 2013, in
Guarujá, São Paulo.
13) Crystals structure of a class P-III metalloproteinase from Bothrops
moojeni venom.
Rehana Masood; Anwar Ullah; Christian Betzel; Raghuvir Krishnaswamy
Arni. XI Congress of the Pan American Section of the International
Society on Toxinology and the XII Congress of the Brazilian Society
of Toxinology, page No. 128, 3-8 November 2013, in Guarujá, São
Paulo.
14) Interaction of quercetin with B. brazili K49 phospholipase A(2);
spectroscopic and molecular modeling studies.
Reetesh kumar; ícaro putinhon caruso; Anwar ullah; Fátima pereira de
souza; Marcelo Andres Fossey; Marinônio Lopes Cornélio; Raghuvir
Krishnaswamy Arni. XI Congress of the Pan American Section of the
International Society on Toxinology and the XII Congress of the
Brazilian Society of Toxinology from 3-8 November 2013, in Guarujá,
São Paulo.
15) Exploring the bonding mechanism of flavonoid (Querecetin) to
phospholipase A2: Fluorescence spectroscopy and computational
approach.
Reetesh Kumar, Icaro P. Caruso, Anwar Ullah, Marinonio L. Cornelio,
Marcelo A. Fossey, Fatima P. de Souza and R. K. Arni, International
Interdisciplinary Science Conference (I-ISC) 2012 on Protein Folding
and Diseases, New Dehli, India.
16) The first crystal structure of a snake venom thrombin-like enzyme
(Jararacussin-1).
Anwar Ullah, Rehana Masood, T. A. C. B, Souza, Mario Murakami and
Raghuvir K. Arni, Structure and function of membrane proteins-a
practical course, 11-22 November 2012. LN-Bio Campinas, Brazil.
17) Structural evidences of the participation of Lys49 residue in the
functioning of “catalytically” inactive phospholipase A2 homologues
and unambiguously definition of dimeric configuration.
A. Ullah, R. Masood, T .A. C. B. Souza, C. Betzel, M. T. Murakami, R. K.
Arni, 45th course Present and Future Methods For Biomolecular
crystallography, Erice Italy, 31 May-10 June 2012.
18) Purification and characterization of serine-proteinases from the
venom of Bothrops alternates and Bothrops moojeni snakes.
ULLAH, A.; Beltran-Abrego, J.R. ; Arni, R. K. ; Caetano, R. C.; Brognaro,
H.; OLIVEIRA, L. M. 4th international conference on natural toxins,
Ismailia Egypt 20-22 December, 2010.
19) Crystal Structure of a P-III Metalloproteinase from the Venom of
Bothrops moojeni.
A. Ullah, P. Oliveira De Giuseppe, M. T. Murakami, F. Pereira De Souza,
C. Betzel. R. K. Arni. Brazilian Biochemistry and Molecular Biology
Society, May 5-8, 2011.
20) Effect of Temperature on Expression Levels and Solubility of Three
Plant Virus Recombinant Proteins in E. coli.
Regatieri, L.J., Ozato Junior, T. Mariutti, R.B., Santana, V.S., Garcia, L.G.,
Ullah, A., Brognaro, H., Souza, F.P., Lopes, M.C. Gaspar, J.O., Arni, R.K.
Brazilian Biochemistry and Molecular Biology Society, May 5-8, 2011.
21) Expression, Purification and Circular Dichroism Analysis of
Grapevine virus B Capsid Protein.
Santana, V.S., Regatieri, L.J., Ozato Junior, T. Mariutti, R.B., Garcia, L.G.,
Ullah, A., Brognaro, H., Souza, F.P.,Lopes, M.C. Gaspar, J.O., Arni, R.K.
Brazilian Biochemistry and Molecular Biology Society, May 5-8, 2011.
22) Purification and characterization of serine proteases of venoms of
Bothrops atrox and Bothrops alternatus that interfere with
coagulation.
Liliane Maria F. Oliveira; Hévila Brognaro dos Santos; Renato Caetano
César; Anwar Ullah; Arni, R. K. Scientific initiation conference
UNESP/IBILCE,2009.
23) Expression, Purification and Refolding of a Coat Protein of Rupestris
stem pitting associated virus.
Mariutti, R.B., Ozato Júnior, T., Santana, V.S., Garcia, L. G., Regatieri,
L.J., Ullah, A.; Cornélio, M.L.A., Gaspar, J.O., Arni, R.K. Brazilian
Biochemistry and Molecular Biology Society, May 5-8, 2011.
24) Cloning, Expression and Purification of a 25K protein from Cole
latent virus. Garcia, L. G., Regatieri, L.J., Mariutti, R.B., Santana, V.S.,
Ullah, A., Santos, H.B., Souza, F.P., Gaspar, J.O., Arni, R.K. Brazilian
Biochemistry and Molecular Biology Society, May 5-8, 2011.
25) Purification, characterization and crystallization of an L-amino acid
oxidase form Bothrops Jararacussu snake.
Anwar Ullah; Rehana Masood; Santana, V.S.; Regatieri, L. J; Garcia, L.
G.; Mariutti, R. B.; Coronado, M. A.; Brognaro, H.; Carolina Wilson;
Liliane, M. F. Oliveira; Murakami, M. T.; Arni, R.K. Simpósio
Interdisciplinar Física + Bioinformática 2011, 9-11 Nov. 2011.
26) Purification, biochemical and structural characterization of an L-
amino acid oxidase form Bothrops Jararacussu snake.
Anwar Ullah; Rehana Masood; Santana, V.S.; Regatieri, L. J.; Garcia, L.
G.; Mariutti, R. B..; Carolina Wilson; Liliane, M. F. Oliveira; Murakami,
M. T.; J. R. B. Abrego, Arni, R.K. TWAS-Biovision.Nxt, 21-25 April 2012,
Alexandria Egypt.
8. Research projects
Research projects completed
2009-2010
a) Structural study of recombinant sphingomyelinase (native and mutant)
from spider venom.
b) Purification, enzymatic characterization and three-dimensional structures
of L-amino acid oxidase from Bothrops venom.
2011-2013
a) Purification, biochemical and structural characterization of phospholipases
A2 from Bothrops venom.
b) Purification, biochemical and structural characterization of serine
proteinases from Bothrops venom.
c) Purification, biochemical and structural characterization of
metalloproteinases from Bothrops venom.
2013-Present
a) Study of interaction between protein-protein and protein–ligand.
b) Co-crystallization of phospholipases, serine proteinases, L-amino acid
oxidases and metalloproteinase with various inhibitors and their functional
and structural analysis/study.
c) Purification and crystallization of recombinant proteins from Brown spider
and Corynebacteria, fungus and plant seeds.
d) Purification and characterization of proteins from seeds.
e) Purification and crystallization of phosphodiesterases.
9. Courses and research supervision
School and college courses
1) 2006-2009- Biology/Chemistry, Jamal English Education Academy, Pabbi
Pakistan.
Graduate courses
1) 2016-2017 General chemistry- Theory and practical course.
2) 2012- Purification and characterization of proteins- Theory and practical
course.
3) 2011- Electrophoresis- Theory and practical course.
Post-graduate courses
1) 2013 till present-Purification and characterization of proteins- Theory and
practical course.
Research supervision
Graduate
1) 2012-2014: Andre Luiz Lira, Purification of proteins, State University of
São Paulo, Brazil (Co-supervisor).
2) 2016-2017- Maryam Qureshi, Extraction and Purification of proteins, CIIT
Islamabad.
Post-graduate
1) 2011-2013: Carolina Wilson: Purification and biochemical
characterization of proteins from the venom of Bothrops leucures snake,
State University of São Paulo, Brazil (Co-supervisor).
2) 2016-presenr: Zafar Hayat, Extraction and Purification of proteins from
bacteria, CIIT Islamabad.
3) 2017-present: Ms. Aimen Khan, Purification and characterization of
proteins from Moringa oleifera seeds, CIIT Islamabad.
10. Participation in Ph. D. defense committee
1) Ph.D. Thesis: Expression, purification and structural characterization of
proteins encoded by two phytovirus, Livia José Regatieri, Doctoral
Program in Microbiology, IBILCE / UNESP, 2014.
2) Thesis: Expression, purification and structural characterization of proteins
encoded by two phytovirus, SBMW and RSPaV, Ricardo Barros Mariutti,
Doctoral Program in Microbiology, IBILCE / UNESP, 2014 (Pre-defense
exam.).
3. Ph. D. Thesis: Expression, purification and structural characterization of
proteins encoded by two phytovirus, SBMW and RSPaV, Ricardo Barros
Mariutti, Doctoral Program in Microbiology, IBILCE / UNESP,
2014.(defense exam.).
4. Ph. D. Thesis. Phosphoryl Transfers of the phospholipase D superfamily:
A quantum Mechanical theoretical study, Luciane Sussuchi da Silva,
Doctoral Program in Molecular Biophysics, IBILCE / UNESP, 2015.
(Defense exam.).
11. Awards
1) Best poster in the conference award, 44th annual meeting of the Brazilian
society for Biochemistry and Molecular Biology (Sbbq) and 23rd
International congress of International Union of Biochemistry and
Molecular Biology (IUBMB), August 24-28, 2015.
2) Best poster in the conference award, 4th international conference on
natural toxins, Ismailia Egypt, 20-22 December 2010.
3) Top 10 posters presentation award, TWAS-Biovision.Nxt, 21-25 April 2012
Alexandria, Egypt.
4) Best subject teacher of the year Award, 2007-2008 Jamal English
Education Academy, Pabbi, Pakistan.
12. TV interviews
1) Enzymes from snake venom can cure cancer. SBT Brazil, 5 May 2011.
2) Enzymes from snake venom can cure cancer. RP TV Brazil, 5 May 2011.
13. Articles Published in newspaper
1) Estruturas presentes no veneno de serpente podem curar doenças,
(Proteins present in snake venom can cure human diseases). Jornal da
UNESP/IBILCE, 2011.
2) How Much Protein in Chicken Liver?
Best Answer. (http://www.blurtit.com/q1449293.html).
3) Jararaca venom, allay against cancer. Diarioweb-Noticias São Jose do
Rio Preto, 7th June, 2011.
14. Scholarships awarded
a) 2008-2013 - TWAS/CNPq, Post-graduate fellowship-Italy/Brazil.
b) 2012 - Biovision fellowship, Egypt.
c) 2013 – ICTP, fellowship South Africa.
d) 2013- 2015 -FAPESP, Post-doc fellowship.
15. REFRENCES
1) Prof. Dr. Christian Betzel
Laboratory of Structural Biology of Infection diseases, Institute of
Biochemistry and Molecular Biology, University of Hamburg, c/o DESY,
Notkestrasse 85, Building 22a, D-22603 Hamburg, Germany.
E-mail: ([email protected])
2) Dr. Ijaz Ali, Post doc [Fulbright]
Associate Professor,
Head of Molecular Virology Group Department of Biosciences, COMSATS
Institute of Information Technology (CIIT) Islamabad Park Road, 44000
Pakistan.
E-mail: [email protected]
3) Dr. Hamid Ali
Assistant Professor,
Department of Biosciences, COMSATS Institute of Information Technology
(CIIT) Islamabad Park Road, 44000 Pakistan.
E-mail: [email protected]
4) Dr. Kifayat Ullah
Assistant Professor,
Department of Biosciences, COMSATS Institute of Information Technology
(CIIT) Islamabad Park Road, 44000 Pakistan.
E-mail: [email protected]
