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DIAGNOSTIC FUNDAMENTALS OF

CLINICAL MICROBIOLOGY

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RIGHT THERAPY

RIGHT DIAGNOSISOF INFECTIOUS

DISEASE

CONTROL

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RIGHT DIAGNOSIS OF

INFECTIOUS DISEASE

CLINIC RADIOLOGY

LABORATORY DIAGNOSIS

P.A. MICROBIOLOGY P.K.

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SPECIMEN

GOOD

Right material

-

Right collection time- Right collection location

- Right collection procedure

- Right storage and transportation- Right quantity

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SPECIMEN COLLECTION

1. PROCEDURE

Sterile : collection location, collection tool, container

2. TIME

Before antibiotic therapy

In certain stadium of disease where microbial agents of diseasecould be found.

tifoid : Salmonellaisolation from blood firs mg of disease

furunkel: mature, or at incision

In the mornings, collected at 24 h

3. LOCATION

Where causative agent would probably be found : most bad,

most deep

paling dalam

4. AMOUNT : according to necessity.

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SPECIMEN STORAGE

Container: sterile dan sealable (screw

capped bottle).

Storage time:must be immediately

processed, may not exceed 24 hours.

Temperature :depends on suspected

bacteria : 4oC, room temperature.

If necessary:transport medium

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SPECIMEN TRANSPORTATION Must be in suitable environment for the

suspected bacteria.

- transport medium

- Room temperature /thermos bottle withice inside

Container may not be carried with bare

hands. Long distance : CONTAINS

MATERIAL THAT CAUSE DISEASE.

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IDENTIFIKASI BAKTERIOLOGIS

SPECIMEN

BACTERIA MACROMOLECULE

Direct

preparation

Bottle/

transport med.

Ag Ab DNA

Microskopic Isolation

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A. Pemeriksaan Mikroskopis

SPECIMEN

DIRECTPREPARATION

WET MOUNT STAINING

G R A M ACID FAST FLUORESCENCE

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Preparat langsung

1. Wet Mount

- morphology

- movement

2. Stained preparation

a. Gram: morphology, Gram characteristic

b. Acid fast: morphology, acid fastcharacteristic

c. Fluorescence: me sensitivity

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BIAKAN BAKTERI

SPECIMEN

BOTTLE/ TRANSPORT MED.

ENRICHMENT MEDIUM

SELECTIVE/DIFFERENTIAL MED

GRAM

Identifi-

cation TES BK AG DNA Tes R

BIAKAN MURNI

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Medium

1. Transport medium = carrier medium

2. Basic medium

3. Enriched medium

4. Enrichment medium5. Selective medium

6. Differential medium

7. Special medium8. Anaerobic medium

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Medium :artif icial environment used to maintain l i fe

and to culture bacter ia outside the host.

Colony :

mass of growing bacter ia on solid medium

Isolated colony:

separated colony, decendant of a single

bacter ial cell .

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Inoculating on solid media