DNA & Nanoscience

DNA

Oligonucleotides

RNA DNA

A-Form B-Form

O

HO OH

BaseHO

Ribose

O

HO

BaseHO

2’-Deoxyribose

Images from Wikipedia

Biological Roles of DNA & RNA

DNA RNA

Store genetic information

Transport information (mRNA)

Build proteins (tRNAs, Ribosomes)

Control gene expression (miRNAs,

piRNAs, siRNAs)

Store genetic information (Viral RNAs)

Catalyze reactions (Ribozymes)

Structural Features of DNA

2 nanometers

Major

Groove

Minor

Groove

1 turn = 10 bases pairs

3.4 nanometers

NN

NH2

ON

NN

N

O

NH2

H

Watson-Crick Base pairs

N

NN

N

NH2

NN

O

O

H

Adenine Thymine

Guanine Cytosine

DNA Replication

Preparation of Oligonucleotides

PCR

Thermal Cycler

(PCR)

DNA

Plasmid DNA

Primers, triphosphates,

and Enzymes

oligonucleotides

Taq DNA Polymerase ATP GTP

CTP TTP

triphosphates

Preparation of Oligonucleotides

Chemical Synthesis

Any Sequence DNA Synthesizer Reagents

DNA Fingerprinting

http://www.pleasanton.k12.ca.us/avhsweb/thiel/bio/labs/csi.html

CSI - Forensic Investigation

DNA in Nanotechnology

Nadrian C. Seeman

New York University

The father of DNA nanotechnology

2D Nanostructures

DNA Origami

DNA in Nanotechnology

P. W. K. Rothemund, Nature 2006, 440, 297 – 302

2D Nanostructures

DNA Origami

DNA in Nanotechnology

DNA in Nanotechnology

DNA in Nanotechnology

TARGET

IMDEA

DNA Extraction Visit to the lab

Tests