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These preliminary results indicate that eBSV could be an adapted marker to precise Musa phylogeography Our aim is to study the integration context of such infectious integrants for Banana streak virus among the diversity of the banana B genome in order to retrace the evolutionary BSV story and understand their impact on host and virus evolution in terms of cost/benefit. To answer this question, we propose to characterize infectious integrants (eBSV) among a representative sampling of Musa balbisiana diversity and to hypothesize a contribution of eBSV towards plant virus resistance through an RNA interfering mechanism versus a maintain of the virus through interspecific crosses. © Nathalie Le Gall The nuclear genome of several plants is invaded by numerous viral sequences. These integrations correspond to accidental events mainly resulting from illegitimate recombination of DNA viruses belonging to the family Caulimoviridae with plant DNA whereas integration into the host genome is not required for viral replication. These integrations are for the most part defective as a result of pseudogenisation driven by the host genome evolution. Conversely, some other integrations named infectious, could release a functional viral genome following activating stresses. eBSGFV A strong correlation exists between southern blots and PCR results PKW hallmark is strongly conserved among the diploid genotypes Positive selection of the infectious allele among the diversity of the banana B genome Pierre-Olivier Duroy 1 , Xavier Perrier 2 , Matthieu Chabannes 1 , Nathalie Laboureau 1 , Marie-Line Iskra-Caruana 1 1 CIRAD/UMR BGPI , 2 UMR AGAP, F-34398 Montpellier cedex 5 [email protected] Sampling of B genome diversity 20 BB banana plants only no geographic information 69 Inter specific hybrids AB, ABB, AAB from the 2 areas of interspecific hybridization origin 13.3 kb eBSGFV- infectious eBSGFV-non infectious 15.6 kb eBSV Goldfinger in PKW - allelic integration ORF1 ORF2 ORF3 IR BSV Dendrogramme from 69 banana plant samples calculated with NJ algorithm and based on 498 Dart markers AAB Popoulou ABB East AAB Pome AAB others AAB Pome AAB Plantain ABB west banksii0620 Agutay Long Tavoy Maia Oa Pahang IDN 110 Fougamou 1 Kluai Namwa Khom Pelipita Burro Cemsa Dole Blue Java Pisang Kepok Bung Daru Bengani Auko Saba Figue Pomme Géante Tay Tia Foconah Prata ana Lady Finger (Nelson) Yangambi no 2 Mai'a popo'ulu moa Popoulou Tigua Tangtang Boung Fu Gamaha Corne 1 Kelong Mekintu Orishele Luba P. Kapas Pisang Raja Bulu Pisang Radjah Kluai Roi Wi Kunaimp Chuoi Mat Pisang Nangka Pisang Ceylan Pisang Slendang Muracho Porp Dimaemamosi Safet Velchi Kunnan Ney Poovan/Figue Pomme(Ekona) Balbisiana (10852) Balbisiana I63-080 Balbisiana HDN 211 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016 Butuhan, intermediate apex Cameroun Eti Kehel Honduras Lal Velchi Singapuri Tani Pisang Batu Pisang Klutuk Pisang Klutuk Wulung Los banos IITA Montpellier IITA I63 IITA 10852 IITA Chinois 1 Chinois 2 Chinois 3 Butuhan Lep Chang Kut Butohan IITA Eti kehel IITA coccinea laterita0627 ornata textilis1072 mannii H. Wendl velutina basjoo Not Named Mainz Dendrogramme from 84 banana plant samples calculated with NJ algorithm based on eBSOLV hallmarks Dendrogramme from 78 banana plant samples calculated with NJ algorithm based on eBSGFV hallmarks AAB others AAB Pome ABB West ABB East AAB Popoulou AAB Plantain (Delanghe et al, ERA 2009) Geographic distribution of the main inter specific triploids banksii0620 Agutay Long Tavoy Maia Oa Pahang IDN 110 Fougamou 1 Kluai Namwa Khom Pelipita Burro Cemsa Dole Blue Java Pisang Kepok Bung Daru Auko Saba Figue Pomme Géante Tay Tia Foconah Prata ana Lady Finger (Nelson) Yangambi no 2 Mai'a popo'ulu moa Popoulou Tigua Gamaha Corne 1 Kelong Mekintu Orishele P. Kapas Pisang Raja Bulu Pisang Radjah Kluai Roi Wi Kunaimp Chuoi Mat Pisang Nangka Pisang Ceylan Muracho Porp Bluggoe Roulo Safet Velchi Kunnan Ney Poovan/Figue Pomme(Ekona) Balbisiana (10852) Balbisiana I63-080 Balbisiana HDN 211 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016 Butuhan, intermediate apex Cameroun Eti Kehel Honduras Lal Velchi Tani Pisang Batu Pisang Klutuk Pisang Klutuk Wulung Los banos IITA Montpellier IITA I63 IITA 10852 IITA Chinois 1 Chinois 2 Chinois 3 Butuhan Lep Chang Kut Butohan IITA Eti kehel IITA coccinea laterita0627 ornata textilis1072 mannii H. Wendl velutina basjoo Not Named Mainz AAB Plantain AAB Popoulou ABB East ABB west AAB others AAB Pome AAA ABB AAB Out Group BB ABB west ABB East AAB Plantain AAB others AAB Popoulou AAB Pome BB AA Agutay Auko Balbisiana (10852) Balbisiana HDN 211 Balbisiana I63-080 Balbisiana LBA-342 Balbisiana0545 Balbisiana0626 Balbisiana1016 banksii0620 basjoo Bengani Blue Java Boung Fu Burro Cemsa Butuhan, intermediate apex Cameroun Chuoi Mat Corne 1 Daru Dimaemamosi Dole Eti Kehel Figue Pomme Géante Foconah Fougamou 1 Gamaha Honduras IDN 110 Kelong Mekintu Kluai Roi Wi Kunaimp Kunnan Lady Finger (Nelson) laterita0627 Long Tavoy Luba Maia Oa Mai'a popo'ulu moa mannii H. Wendl Muracho Not Named Mainz Orishele ornata Pahang Pelipita Pisang Ceylan Pisang Kepok Bung Pisang Nangka Pisang Radjah Pisang Raja Bulu Pisang Slendang Popoulou Prata ana Saba Safet Velchi Singapuri Tangtang Tay Tia textilis1072 Tigua velutina Yangambi no 2 Pisang Klutuk Wulung PKW (BB) as a model 23.2 kb eBSOlV-non infectious eBSOlV-infectious 22.9 kb eBSV Obino l’Ewai in PKW - allelic integration Specific hallmark for each eBSV integration in PKW PCR markers Southern blot fingerprints eBSOLV A faint correlation exists between southern blots and PCR results PKW hallmark is restricted to PKW clade Integration loci are highly conserved A large area of differentiation with a lot of changes and mutations resulting on identification of novel alleles in the west hybridization area Positive selection of the infectious allele eBSGFV eBSOLV Infectious allele Non Infectious allele Non eBSGFV allele Infectious allele Non Infectious allele Non eBSGFV allele South-East Asia
