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al - EnglishioSpectrometer® 6135/6136/6137EN)manual - English

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Eppendorf BioSpectrometer® 6135/6136/6137Service manual - English

Copyright© 2014 by Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part

of this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany.

Eppendorf BioSpectrometer®, Eppendorf SpectraZoom® and UVette® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Cy is a registered trademark of GE Healthcare UK Ltd., Buckinghamshire, UK.

Hellma® is a registered trademark of Hellma GmbH & Co. KG, Müllheim, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

This product is manufactured under license to issued U.S. patent 6,122,052.

6135 920.019-02/022014

3Table of contents

Eppendorf BioSpectrometer® 6135/6136/6137

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.2 Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.3 Hazard to persons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.4 Risk of equipment damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3.5 Hazards during repairs and shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

4 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1.1 Entering text . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.3 Troubleshooting list for Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.4 Result flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.5 Service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.1 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.2 Activating service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.3 "Function check" subgroup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.5.4 "Software" subgroup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.5.5 "Information" subgroup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.5.6 "Adjustment" subgroup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.6 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.6.1 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.6.2 Activating customer service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.6.3 "Device settings" subgroup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.6.4 "Device calibration" subgroup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6 Disassembly/assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 616.1 Dismantling or replacing components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.1.1 Required tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.1.2 Disassembling the housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.1.3 Replacing the lamp with the lamp holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.1.4 Replacing the PCB BioSpectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.1.5 Replacing the switching power supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

6.1.6 Replacing the display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.1.7 Replacing the keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.1.8 Dismantling the diffraction grating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Table of contentsEppendorf BioSpectrometer® 6135/6136/6137

English (EN)4

6.1.9 Dismantling the cuvette shaft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.1.10 Replacing the fiber optic cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.1.11 Replacing the PCB photo diode array . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.1.12 Replacing the photo diode array. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.1.13 Replacing the mirror . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.1.14 Disassembling the fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

7 Alignment/adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 777.1 Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

7.1.1 Required tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

7.1.2 Adjusting the diffraction grating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

7.1.3 Adjusting the fiber-optic cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

8 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 798.1 Updating the device software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

9 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 819.1 Layout plans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

9.1.1 BioSpectrometer basic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

9.1.2 BioSpectrometer kinetic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

9.1.3 BioSpectrometer fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8510.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10.2 Shipping the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10.3 Service schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10.4 Description of service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

11 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8911.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.4 Photometric properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.5 Incubation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.6 Further technical parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.7 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

12 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9312.1 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

12.1.1 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

12.1.2 Electrical/electronic equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

12.1.3 Light path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

12.1.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

12.1.5 Auxiliary aids. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

13 Technical information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

5Operating instructions


English (EN)

1 Operating instructions1.1 Using this manual

Ensure that you have a copy of the latest versions of the service manual and operating instructions.

Check this using the version numbers at www.eppendorf-support.com and www.eppendorf.com.

Read the service manual before starting work on the device.

Read the chapters "Installation" and "Operation" of the operating manual.

Observe the safety instructions in the operating manual.

1.2 Danger symbols and danger levels

1.2.1 Danger symbols

1.2.2 Danger levels

The safety notes in this service manual contain the following degrees of danger:

Biohazard Explosion

Electric shock Cuts

Hazard point Material damage

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

Operating instructionsEppendorf BioSpectrometer® 6135/6136/6137

English (EN)6

7Product description


English (EN)

2 Product description2.1 Main illustration

Abb. 2-1: Front and rear view

Fig. 2-1: Front and rear view

The name plate is located at the bottom left on the underside of the device.

1 Display

2 Cuvette shaft

3 Cuvette shaft cover

4 USB connection for USB stick

5 Power switch

6 Fuse holder

7 Power connection

8 USB connection for PC

9 Connection for RS-232 printer

10 Operating controls

standardsample

mno6jkl

5

13abc

2def4

ghi

pqrs7

tuv8

wxyz9

method

function

µ %0

exit

delete

enter blank

a b s o r b a n c e

absorbance

h e i g h t8 . 5 m m

10 9 8 7 6 5 4

1 2 33

Product descriptionEppendorf BioSpectrometer® 6135/6136/6137

English (EN)8

9Safety


English (EN)

3 Safety3.1 User profile

The specialist entrusted with maintenance, repair or testing must meet the following prerequisites:

• Successful participation in service trainings with certification by Eppendorf AG for the product that is to

be maintained, repaired or tested.

• Qualification as a specialist with knowledge of the applicable local and international standards.

• Qualification to evaluate the work assigned to him.

• Recognition of potential hazards and how to prevent or correct them.

3.2 Liability

The Eppendorf service partner entrusted with maintenance, servicing or testing is liable for ensuring that

all work is carried out in a professional manner.

The constructional features of the device must not be modified by servicing, maintenance or testing.

The device must always be as safe as in its original state.

Only accessories and original spare parts recommended by Eppendorf as well as measuring and test

equipment recommended by Eppendorf may be used for any maintenance, servicing or testing work.

3.3 Hazard to persons

DANGER! Electric shock.

Switch the device off.

Unplug the device.

Then begin repairing, servicing or cleaning the device.

DANGER! Electric shock.

Switch off the device and disconnect the power plug before opening the device to replace

the lamp or fuses. These tasks may only be performed by appropriately trained staff.

DANGER! Danger to life due to live parts.If you carry out work when the device is open or energized, you may come into contact with

live parts.

Do not touch the device or its components.

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device for processing any substances which could generate an explosive

atmosphere.

SafetyEppendorf BioSpectrometer® 6135/6136/6137

English (EN)10

3.4 Risk of equipment damage

WARNING! Lethal voltage in the area of the xenon lamp.The connections of the xenon lamp, its supply lines and connections on the PCB carry a live

voltage of 1000 V.

Never touch components that are under high voltage.

Only use tools that are specially protected against high voltages to adjust the xenon lamp.

WARNING! Burns due to ultraviolet radiation.The xenon lamp emits intensive ultraviolet radiation. This intensive ultraviolet radiation may

cause burns to the skin and eyes.

Wear protective glasses against ultraviolet radiation.

Protect your skin from the ultraviolet radiation.

Do not look into the light beam of the lamp.

WARNING! Risk from incorrect supply voltage

Only connect the device to voltage sources which correspond to the electrical

requirements on the name plate.

Only use sockets with a protective earth (PE) conductor and suitable power cable.

NOTICE! Damage to electrical components due to electrostatic discharge.Handling electrical components creates electrical fields. The electrical components are

destroyed when these fields are discharged. Minimize the build-up of electrostatic fields.

Store and transport sensitive components and assemblies in antistatic or conductive

packing.

Wear grounding strips, antistatic clothing and antistatic safety boots.

Use dissipative surfaces.

Grip the component on the edges.

Do not touch any protruding connections or conductors.

Prevent components from becoming electrostatically charged on plastics.

NOTICE! Damage due to overheating.

Do not place the device near heat sources (e.g., heater, drier compartment).

Do not expose the device to direct sunlight.

Guarantee unobstructed air circulation by maintaining a distance of at least 5 cm, on all

sides of the device, to the adjacent devices and the wall, and by keeping the device base

clear.

To keep the ventilation slits for the lamp at the rear of the device clear, do not place any

objects on the device.

11Safety


English (EN)

3.5 Hazards during repairs and shipping

NOTICE! Damage to the xenon lamp from contamination.Grease and dirt on the glass part can lead to premature failure of the xenon lamp.

Only touch the xenon lamp with gloves.

Clean dirty xenon lamps before installing them.

To do this, moisten a soft cloth with pure alcohol.Use it to clean the glass part of the Xenon

lamp.

NOTICE! Corrosion from aggressive cleaning agents and disinfectants.

Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.

Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer

period of time.

NOTICE! Damage to electronic components due to condensation. Condensate can form in the device after it has been moved from a cool environment to a

warmer environment.

After installing the device, wait at least for 2 h. Only then connect the device to the mains.

WARNING! Infection by contaminated material.There may be contaminated material on the device and accessories.

Work may only be completed on a decontaminated device.

Find out more about contamination risks before beginning work.

Check the device decontamination certificate.

Wear personal protective equipment (protective gloves, protective goggles).

NOTICE! Damage as a result of incorrect packing.Eppendorf AG is not liable for damage caused by improper packing.

The device may only be stored and transported in its original packaging.

SafetyEppendorf BioSpectrometer® 6135/6136/6137

English (EN)12

13Operation


English (EN)

4 Operation4.1 Overview of operating controls

Abb. 4-1: Control panel of the BioSpectrometer

Fig. 4-1: Control panel of the BioSpectrometer

Key: Function

Keypad: Enter digits and text.

Keys 1 to 9 as well as 0: When entering text, next to numbers you also can

enter letters and special characters by pressing the key several times.

Alternatively, you can switch to a displayed keyboard with the [Keyboard] key.

Outside of entry fields: Call up method selection.

Outside of entry fields: Call up function selection.

Softkey: Select functions.

The key assignment changes along with the software dialog. The current

function is displayed directly above the key on the display.

OperationEppendorf BioSpectrometer® 6135/6136/6137

English (EN)14

4.1.1 Entering text

You can enter texts when assigning method names and result units. Restriction: Only digits, letters and the

underscore "_" are allowed for method names.

Move the cursor to the left, right, up, down.

• Navigation between input fields.

• and keys inside an entry field: Navigate within the character string.

• and keys in a result display: Navigate between the sample results of

the series of measurement.

• and keys within a graph: Navigate on the x-axis of the graph, e.g. for

displaying the wavelength-dependent absorbance values in a scan.

and keys in an absorbance wavelength spectrum: Change image

section (SpectraZoom procedure).

Exit the current selection for the next higher level.

Delete entry. Within a sequence of signs, the sign on the left of the cursor is

deleted

• Call up selected method or function.

• Open the selection list.

• Confirm entry or selection.

Start standard measurement.

Start blank measurement.

Start sample measurement.

Entry via keyboard:

Use the and cursor keys to navigate within the

entry field and to change single positions in the

name.

Softkeys:

• [Keyboard]: Display keyboard.

• [abc]: Change between upper and lower case

letters when making entries with the keypad.

• [Save]: Save entered text.

• [Cancel]: Cancel text input.

Key: Function

15Operation


English (EN)

Entry via the displayed keyboard:

Use the cursor keys to select the displayed signs and

respectively confirm your selection with the enter

key. As for a PC key pad, you can use the "Shift"

resp. the "Caps Lock" key for changing the

capitalization for the next entry or for all following

entries.

Softkeys:

• [Numbers]: Switch to entry using the keyboard.

• [Save]: Save entered text.

• [Cancel]: Cancel text input.

OperationEppendorf BioSpectrometer® 6135/6136/6137

English (EN)16

17Troubleshooting


English (EN)

5 Troubleshooting5.1 General errors

Error Possible cause Remedy

Measuring results are

imprecise.

• Reagent is past its shelf

life.

Ensure that the reagent is still within its

shelf life and properly prepared.

• Reagent has not been

prepared properly.

Use clean demineralized water of adequate

quality for preparation if required.

• Pipetting is not correct. Ensure that the pipette is calibrated and

that pipetting is being performed correctly.

• Incubation procedure

before measurement is

incorrect.

If the method procedure requires

incubation before the measurement,

ensure that the temperature and time for

incubation are correctly observed.

• The cuvette is

contaminated.

Clean and rinse the cuvette. When

replacing a cuvette, pay attention that the

optical window of the cuvette remains

clean and that you do not touch it with your

fingers.

If the cuvette window has become soiled

from fingerprints, wipe it clean using a

lint-free lab cloth soaked in ethanol or

isopropanol.

• The cuvette is not filled

completely with

measuring solution, and it

contains bubbles.

Ensure that the required minimum volume

of the cuvette for a measurement is

reached and that no bubbles are in the

measuring solution.

• Turbidity of the

measuring solution.

Centrifuge the turbid measuring solutions

containing particles and use the clear

supernatant.

• Spectrophotometer is

drifting.

Contact Eppendorf Service.

Observe the ambient conditions.

Prevent temperature changes.

• Cuvette shaft is dirty. Clean the cuvette shaft.

• Fluorimetry: Interfering

substances reinforce or

weaken the fluorescence

signal.

Remove the interfering substances.

If the interfering substances cannot be

removed, fluorimetry measurement

technology cannot be used.

• Fluorimetry: The cuvette

shaft cover is not closed.

Close the cuvette shaft cover prior to

measurement.

TroubleshootingEppendorf BioSpectrometer® 6135/6136/6137

English (EN)18

5.2 Error messages

You can exit device displays with error messages using the [OK] softkey.

System errors require an evaluation by the Technical Service. These errors are shown in English (System error …). Please contact Technical Service in these cases. Other error messages, for which you can carry

out troubleshooting measures, are illustrated in the table below.

The measuring results are

not correct.

• The method has not been

programmed correctly.

Ensure that the method parameters are

entered correctly.

• The standard solution has

not been prepared

correctly.

Ensure that the correct standard is used

and that the measuring solution for the

standard is prepared correctly.

• The absorbance of the

reagent is drifting.

For instable reagent absorbance and end

point methods: When measuring a long

series of samples, measure the reagent

blank value not only at the beginning but

also during the sample series. If the

reagent blank value drifts strongly, the

reagent is not appropriate for error-free

measurements and has to be replaced by a

new reagent.

• The cuvette is not

positioned correctly.

Position the cuvette in the cuvette shaft so

that the optical window points towards the

direction of the light path.

Photometry light path: from back to front

Fluorimetry light path: from right to left

Symptom/message

Cause Remedy BioSp BioSp_fluor

BioSp_kin

Self test failed. • Cuvette shaft cover was

open during self test.

• The cuvette shaft was not

empty during the self test.

Repeat the self test with

empty cuvette shaft and the

cuvette shaft cover closed.

• Device is faulty. Contact Eppendorf Service.

File export

failed.

During data export:

• USB stick improperly

formatted or faulty.

• USB stick removed from the

device too early (during the

export).

Reformat or replace the USB

stick.

Reconnect the USB stick and

repeat the export.

Error Possible cause Remedy

19Troubleshooting


English (EN)

Failed to

initialize

printer.

• Printer not connected or

switched off.

• Printer not configured

correctly.

Connect the printer and

switch it on.

Reconfigure the printer.

For a correct configuration of

the printer settings refer to the

installation description.

Blank

measurement:

An intensity on

a pixel that

influences the

main, auxiliary

or scan

wavelength is

too low.

• The absorbance of the

blank solution used for the

blank measurement is too

high.

• Incorrect or turbid blank

solution.

• For scans: Wavelength

range is too large, because

the sample is very strongly

absorbed in part of the

wavelength range.

Check the blank solution and

remeasure the blank if

required.

For scans: Match the

wavelength range to the

sample spectrum.

Blank

measurement:

The emission

at the

measurement

wavelength is

too high.

• The fluorescence of the

blank solution used for the

blank measurement is too

high.

• Incorrect or turbid blank

solution.

Check the blank solution and

remeasure the blank.

X

The entered

name is not

valid.

• Error when entering the

name. Different causes are

possible. For the precise

cause please see the

information in the help box.

See information in the help

box.

A method (or

folder, dye,

protein,

nucleic acid, or

unit) with this

name already

exists.

• The name under which the

method was saved has

already been used for a

different method in the

same folder.

• The message also appears

after editing names already

given to a folder or to a

nucleic acid (dye, protein,

concentration unit) (under

General Method Parameter).

Assign a different name.

Symptom/message


BioSp_kin


English (EN)20

The following

parameter

values are not

defined in

General Method Parameter:

• When opening a method

with parameters which

access General Method Parameter, the system

determined that at least one

parameter (dye, nucleic

acid, protein, unit) does not

exist there anymore, so

probably has been deleted.

Select a different parameter

from the existing list. If

necessary, program a new

list entry in General Method Parameter in order to be

able to use it when

programming a method.

The value of

the parameter

marked with * is not defined

in the Gen.

Param. Please

correct the

parameter.

This error message appears

when editing method

parameters.

• Parameter in General Method Parameter is not

defined.

Select a different parameter

from the existing list. If

necessary, program a new

list entry in General Method Parameter in order to be

able to use it when

programming a method.

Invalid zoom

interval.

During the Zoom process with

free entry of limits ([Free])

softkey):

• The zoom area is below the

lower limit.

Enter the values so that the

interval does not fall below

the range limits of 0.02 A

and 10 nm.

X

The entered

standard

concentrations

are not

monotonically

increasing

resp.

monotonically

falling. Please

correct the

standard

concentrations.

• See the error text. Enter the standard

concentrations so that the

first standard receives the

lowest concentration and the

other standard

concentrations form an

increasing sequence.

At least two of

the entered

standard

concentrations

are identical.

Please correct

the standard

concentrations.

• See the error text. Enter the standard

concentrations so that the

first standard receives the

lowest concentration and the

other standard

concentrations form an

increasing sequence.

Symptom/message


BioSp_kin

21Troubleshooting


English (EN)

The measured

values are not

strictly

monotone!

• Error when measuring a

standard series: The

measured absorbance

values of the standard

series are not continuously

increasing or decreasing.

Repeat the standard

measurements or delete the

single, incorrectly measured

standard result.

The ID cannot

be set.


sample ID. Different causes

are possible. For the precise




box.

The dilution

cannot be set.


dilution. Different causes

are possible. For the precise




box.

Calculation not

possible

because of

division by

zero.

Absorbance

result or

Formula "b"

parameter is

zero.

• An absorbance result was

divided by a "zero" value

during the evaluation of a

Division type method (Dual wavelength method

group). This is not

mathematically permissible.

Check the reagents and

samples used and repeat the

measurement.

Do not enter "zero" as a

value for the Formula b

parameter.

X

There is only

one

measurement

left to be

performed in

this series of

measurement.

The maximum

number of

measurements

within one

series of

measurements

has been

reached.

• The number of

measurements in one

measuring series is limited

to 99.

Start a new series of

measurement after

maximally 99

measurements.

Symptom/message


BioSp_kin


English (EN)22

Invalid zoom

interval!

Error in the process results

method step in the Zoom

mode.

Permissible zoom range for the

wavelength scale:

• Wavelength interval at least

10 nm

• Entries for wavelengths

only within the range

programmed in the

parameters for the method.

Permissible zoom range for the

absorbance scale:

• Absorbance interval at least

0.02 A

• Upper and lower limit for

absorbance interval +3 A or

–3 A

Please observe the stated

limits in the zoom

procedure.

X

Device

configuration

was changed

from … to … .

• A BioSpectrometer kinetics

is not detected as the

kinetic variant but as a

BioSpectrometer basic. The

kinetic methods are

therefore not displayed.

Switch the device off and

back on again. If the error

occurs again: Contact

Technical Service.

X

The

temperature

control is

faulty. Please

program the

method

without

temperature

control or

cancel the

method.

• The temperature control of

the device is faulty.

Contact Eppendorf Service.

Until the temperature

control has been repaired

only use methods for which

no temperature control has

been programmed.

X

The ambient

temperature is

too high.

For kinetic methods with

temperature control:

• The ambient temperature

measured by the device is

above the specified range.

Make sure that the ambient

temperature is within the

range specified for the

operation of the device.

X

Symptom/message


BioSp_kin

23Troubleshooting


English (EN)

5.3 Troubleshooting list for Service

Linear

regression

could not be

applied to all

measurements.

• For kinetic methods, the

time frame for evaluation

with linear regression was

changed in the process results method step, and

the change should be

extended to all measuring

results. The required

number of measuring

points, however, was not

available for at least one

sample result.

Only change the time frame

for evaluation with linear

regression in the process results method step for

samples with sufficient

measuring points.

X

Symptom/message


BioSp_kin

Method

parameters

could not be

loaded!

• File system defective Restore factory settings.

Initialising

BioSpectromet

er 6135

FAILED

• Major software error

• Major hardware error

1. Carry out software update.

2. Replace the main board.

No response

from

spectrometer

unit.

• Spectrometer unit firmware

faulty.

• Spectrometer unit or cable

connection defective.

1. Restart.

2. Check cable connections.

3. Replace Spectrometer unit.

Could not

mount USB

storage.

• USB stick missing

• USB stick is defective.

• USB stick is missing or has

incorrect file system.

Connect operative USB stick

to FAT32 file system.

Could not

unmount USB

storage!

• USB stick removed during

access (e.g., export,

backup, …).

Reconnect USB stick and

carry out the process again.

Could not find

method: XXX.

Please delete

this method

and create a

new one!

• File system defective Restore factory settings.

Symptom/message


BioSp_kin


English (EN)24

Pixel

wavelength

assignment

missing.

Please call

Eppendorf

service!

• Missing optical adjustment. Carry out optical

adjustment.

Error during

loading of

method. The

file may be

corrupted.

Please call EP

Service if you

cannot recover

the file!

• Method file faulty.

• File system defective.

1. Delete method.

2. Restore factory settings.

Method could

not be loaded.



1. Delete method.


Various SPU

errors

• Spectrometer unit firmware

faulty.

• Spectrometer unit or cable

connection defective.

1. Restart.

2. Check cable connections.

3. Replace Spectrometer unit.

Error while

initializing

processing of

results.



1. Delete method.


Result could

not be loaded.

Please try

again after

restart.

• Result faulty.


Delete result.

Restore factory settings.

Spectrometer

Unit did not

produce a

result.

• Cable connection between

the basic printed circuit

board and photo diode

array printed circuit board

is defective.

• Photo diode array PCB

defective.

• Basic printed circuit board

defective.

Check the cable connection

between the basic printed

circuit board and photo

diode array printed circuit

board is defective.

Replace the photo diode

array printed circuit board.

Replace the basic printed

circuit board.

Symptom/message


BioSp_kin

25Troubleshooting


English (EN)

An invalid

method

definition

prevents the

completion of

the result

computation.

The method

resource file

might be

corrupt.



1. Delete method.


Invalid

filename.

• File system defective. Software update


Could not eject

USB storage.

• USB stick removed during

access (e.g., export,

backup, …).

Reconnect USB stick and

carry out the process again.

Could not

prepare PC

storage.

• File system defective. Restore factory settings.

Replace the main board.

Error opening

file XXX


Resource data

corrupted.

Backup was

being restored.


Backup could

not be

restored.

Please call

Eppendorf

Service.


Method could

not be saved.

• File system defective

• Not enough memory.

Delete old measuring results

and methods.


Serial number

does not match

hardware type.


is configured for D30.


circuit board.

Key pad seems

to be

damaged,

please replace.

• Key pad defective. Replace key pad.

Unable to save

settings.




and methods.


Symptom/message


BioSp_kin


English (EN)26

Could not read

filter settings

from USB

storage.

• USB stick with filter data is

missing.

• USB stick with filter data is

defective.

• USB stick does not contain

any valid filter data file.

Connect USB stick with valid

filter data.

Out of memory • File system defective.



and methods.


Could not set

serial number

• Serial number invalid.

• EEPROM not writable.

Check serial number.


circuit board.

Could not set

flash count



Carry out software update.


Could not read

temperature

from

temperature

unit.

• Tempering deactivated

because of error (e.g.,

overheating, defect).

1. Restart.

2. Check the cable connections

to the kinetic module.

3. Replace the kinetic module.

X

Temperature

control error

• Tempering deactivated

because of error (e.g.,

overheating, defect).

1. Restart.

2. Check the cable connections

to the kinetic module.

3. Replace the kinetic module.

X

Cuvette

temperature is

too high (max.

X °C).

• Cuvette shaft temperature

is too high.

Allow the cuvette shaft to

cool off.

X

Temperature

difference to

reference

sensor too

high

• Sensor faulty


defective

Replace sensor.


circuit board.

X

Difference

between

ambient

temperature

and

destination

temperature is

too high.

• Difference between the

inside temperature of the

housing and the set

temperature is too high.

• Sensor faulty


defective

The device may only be

operated in preset

temperature ranges.

Replace sensor.


circuit board.

X

Symptom/message


BioSp_kin

27Troubleshooting


English (EN)

Temperature

cannot be

reached.

Temperature

unit will be

disabled.

• Cable connection defective.

• Peltier element defective.

Check cable connection.

Replace Peltier element.

X

Temperature

correction

could not be

saved.



Software update


X

No

temperature

correction

function found.

• Temperature correction file

defective.


Carry out temperature

adjustment.

X

Fan failure • Fan is blocked.

• Fan is defective.

• Cable connection defective.

Check fan and cable

connection.

Replace fan and cable

connection.

X

The tempering

timeout is

greater than

1 h.

• Incorrect settings in the

configuration files (.xml).

Carry out software update. X

Fluorescence

unit did not

produce a

result.

• Old software version


basic printed circuit board

and fluorescence module

defective.

• Fluorescence module

defective.


defective

Software update


between basic printed

circuit board and

fluorescence module.

Replace the fluorescence

module.


circuit board.

X

Could not set

sensitivity for

fluorescence

measurement.





defective.


defective.


defective

Software update



circuit board and



module.


circuit board.

X

Symptom/message


BioSp_kin


English (EN)28

Error setting

fluorescence

wavelength.





defective.


defective.


defective

Software update



circuit board and



module.


circuit board.

X

Error reading

result from

fluorescence

unit.





defective.


defective.


defective

Software update



circuit board and



module.


circuit board.

X

Response

timeout.





defective.


defective.


defective

Software update



circuit board and



module.


circuit board.

X

Standard X for

wavelength

X nm did not

produce linear

values.

• Adjustment error Repeat adjustment. X

Could not

compute

intersection

triangle.

• Adjustment error Repeat adjustment. X

Symptom/message


BioSp_kin

29Troubleshooting


English (EN)

5.4 Result flags

Warnings and error messages for results are displayed in the bottom right of the help box. The header bar

of the Help box is highlighted yellow for warnings and red for error messages.

Warnings: Decide whether the result is useful for you while taking the displayed warning into

consideration.

Error messages: No result is displayed; the reason is shown in the error message.

Symptom/message


BioSp_kin

The standard

curve is not

monotone.

Please select

another Curve

Fit.

• No usable result was

returned during the

evaluation of a standard

curve using the "spline

interpolation", "quadratic

regression" or "cubic

regression" Curve Fit procedures.

Select a different Curve Fit procedure.

Some

absorbance

values for

secondary

wavelengths

are too high or

are not

displayed.

• For at least one secondary

wavelength, the absorbance

exceeded the measuring

range.

• Secondary wavelengths are

not needed for calculating

the concentration result.

They are used for different

purposes. For example,

dsDNA method:

absorbance at 280 nm for

the calculation of ratios 260/280.

• Turbidity of the measuring

solution

• Measurements at the limits

of the photometric

measuring range.

If the absorbance values of

the secondary wavelengths

are relevant: Dilute the

sample or remove the

turbidity via centrifugation

and repeat the

measurement.

The result is

outside the

range of the

standard

concentrations

.

• For methods with

evaluation via standard

curves (nonlinear

evaluation method): The

sample result is up to 5 %

outside of the standard

concentration range.

Accept the measurement

result, or remeasure the

sample under conditions

under which the result is

within the range of the

standard concentrations

(dilute sample or modify

standard concentrations and

remeasure).


English (EN)30

The coefficient

of

determination

is <0.8.


evaluation of standard

series via the regression

procedure: The coefficient

of determination for the

regression evaluation

indicates a significant

deviation of the measuring

points from the regression

line.


solution.


of the photometric

measuring range.

Accept the result of the

standard evaluation or

remeasure the standards.

Make sure the measuring

solutions are clear.

The coefficient

of

determination

for the

regression

evaluation of

the standard

series is < 0.8.


evaluation of standard

series via the regression

procedure: If the regression

evaluation for the standard

series was nonlinear, but

the standard evaluation was

accepted by the user, a

warning appears after

samples have been

measured.

Use the sample results with

the reservation mentioned

or repeat the measurement

of the standard series and

samples.

Scan: Some of

the measured

absorbances

are too high

and are not

displayed.

• For at least one scan

wavelength, the absorbance

exceeded the measuring

range.


solution.


of the photometric

measuring range.

If the non-displayed areas of

the scan are relevant: Dilute

the sample or remove the

dilution via centrifugation

and repeat the

measurement.

The

measurement

is not

complete.

• Kinetic procedure: You have

prematurely canceled the

measurement using the

[Stop] softkey. If at least 2

measuring points are

available, a result is

calculated and displayed.

Accept the measuring result

with the reduced measuring

time or measure again with

a longer measuring time.

X

Symptom/message


BioSp_kin

31Troubleshooting


English (EN)

The kinetics

are nonlinear:

The coefficient

of

determination

is < 0.95.

• Kinetic procedure with

"linear regression"

measuring procedure: The

coefficient of determination

for the regression

evaluation indicates a

significant deviation of the

measuring points from the

regression line.


solution.


of the photometric

measuring range.

• Activity concentration of

the enzyme too high.

Accept measuring result or

remeasure the sample.

Before repeating the

measurement, evaluate the

reason for the non-linearity

and act accordingly (e.g.,

clear the measuring solution

by centrifugation or dilute

the sample).

X

The kinetics

are nonlinear

for at least one

standard: The

coefficient of

determination

is <0.95.

• Kinetic procedure with

"linear regression"

measuring procedure and

evaluation procedure via

standards: The coefficient

of determination for the

regression evaluation of at

least one standard

measurement indicates a

significant deviation of the

measuring points from the

regression line.


solution.


of the photometric

measuring range.

• Activity concentration of

the enzyme too high.

Accept measuring result or

remeasure the standard.

Before repeating the

measurement, evaluate the

reason for the non-linearity

and act accordingly (e.g.,

clear the measuring solution

via centrifugation or use the

standard with low

concentration).

X

During the

kinetics

measurement,

the

temperature

was outside of

the allowable

range.

• Ambient temperature

outside the specified range.

• The temperature control is

faulty.

Measure the sample at

ambient temperature within

the specified range (15°C to

35°C). If the warning

nevertheless appears,

contact Eppendorf Service.

X

Symptom/message


BioSp_kin


English (EN)32

Absorbance at

the measuring

wavelength is

too high.

Emission at the

measuring

wavelength is

too high.


solution.

• Optical surfaces of the

cuvette are soiled.

• Cuvette has been inserted

into the cuvette shaft facing

the wrong direction.

• Too high absorbance of

measuring solution.

• Photometry: Too high

absorbance of the

measuring solution.

Fluorimetry: Too high

emission of the measuring

solution.

Measure again considering

the possible causes.

The calculated

result is

negative.

• Measuring solution not

prepared correctly.

• The incorrect factor has

been entered (wrong

algebraic sign).



At least one of

the results is

negative.

• For methods with several

results (e.g., Dye labels).

• Measuring solution not

prepared correctly.

• The incorrect factor has

been entered (wrong

algebraic sign).



X

The result has

more than 6

pre-decimal

places.

• Very high sample

concentration.

• Concentration unit does not

match the expected range

of the sample

concentrations.

Dilute sample and measure

again.

Change the concentration

unit (Parameter Unit) and

measure again.

The result is

more than 5 %

outside of the

standard

concentration

range.


evaluation via standard

curves (nonlinear

evaluation method):

The sample result is more

than 5 % outside of the

standard concentration

range.

Remeasure the sample

under conditions under

which the result is within

the range of the standard

concentrations (dilute

sample, modify standard

concentrations and

remeasure).

Symptom/message


BioSp_kin

33Troubleshooting


English (EN)

• Calculation

not

possible

because of

division by

zero.

Absorbance

result is

zero.

• Calculation

error.

Division by

zero.

• The evaluation required

dividing by an absorbance

result with the value of

"zero". This is not

mathematically

permissible.

Examples: Calculation of a

factor at one-point

calibration; calculation of a

260/280 ratio with nucleic

acid measurements.



measurement.

Calculation not

possible

because of

division by

zero.

Absorbance

result or

parameter

formula b is

zero.

• An absorbance result was

divided by a "zero" value

during the evaluation of a

Division type method (Dual wavelength method

group). This is not

mathematically

permissible.



measurement.

Do not enter "zero" as a

value for the Formula b

parameter.

X

Symptom/message


BioSp_kin


English (EN)34

5.5 Service functions

5.5.1 Navigation

1. Use the up and down arrow keys to select the entries in a column (groups, functions).

2. Use the left and right arrow keys to switch from one column to another.

5.5.2 Activating service functions

Proceed as follows to activate the service functions:

1. Switch the BioSpectrometer on.

2. Press the Function softkey.

The display with the customer service functions

appears.

3. Press the Sample test key and 9 numeric key at

the same time.

The password query display appears.

35Troubleshooting


English (EN)

5.5.3 "Function check" subgroup

4. Enter the password "4711" using the numeric

keys.

5. Press the OK softkey to confirm the entry.

The display with customer service functions and

service functions appears.

The Cancel softkey cancels the log-in process and

the display with customer service functions

appears.

6. Select the Service entry in the main directory.

Switch to the Function check subdirectory in the

Sub Groups column.


English (EN)36

5.5.3.1 "Check keyboard" service function

5.5.3.2 "Display" service function

1. Select the Keyboard service function and access it

using the Enter key.

2. Press all of the keys one after the other.

The name of the key that was pressed appears in

the display.

The image shows the display after the Standard

key has been pressed.

3. Press the Finish softkey to confirm the entry.

Select the Display service function and access it


The depicted color pattern appears in the display

in horizontal and vertical layouts.

37Troubleshooting


English (EN)

5.5.3.3 "Interfaces" service function

Required equipment• USB connecting cable (USB A - USB B)

• Short-circuit plug (5075 851.563)

5.5.3.4 "Flash bulb" service function

The Back softkey can be used to return to the previous function.

Press the Abort softkey to exit the service function and return to the function menu.

Flash bulb

1. Select the Interfaces service function and access it


2. Insert the short-circuit plug (5075 851.563) into

the RS232 interface.

3. Connect the USB interface on the rear of the

device to the USB interface on the left side using

a commercial USB cable.

4. Press the Check key to start the test.

After the test has been successfully completed,

the display switches from UNTESTED to PASSED.

5. Press the Exit softkey to confirm the entry.

1. Select the Flash bulb service function and access

it using the Enter key.

2. Start the reference measurement using the Check

softkey.

Each color-coded cuvette piece must seamlessly

connect to the next piece.

3. Replace light source if irregularities occur in the

curve shape (jumps).

Possible causes of an irregular curve shape

include an old Xenon lamp, a misaligned lamp

holder or a defective reference sensor.


English (EN)38

Dark measurement

Intensity test

Raw data

Dark current subtracted

4. Press the Next> softkey to switch the dark

measurement.

5. Start the dark measurement using the Check

softkey.

Two straight lines can be seen in the diagram if

no stray light enters the device.

Stray light or a missing light guide plate create

two curve shapes in the diagram.

6. Press the Next> softkey to switch to the intensity

tests.

7. Select the Raw data service function and start the

measurement using the Check key.

This help function shows the device's

unprocessed measurement data in the diagram. It

is used for the error search during development.

8. Select the Subtract dark current service function

and start the measurement using the Check key.

This help function shows the result of the

calculation of the raw data and dark current data.

It is used for the error search during

development.

39Troubleshooting


English (EN)

Reference value incl.

Fully incl. in calc.

Distribution test

Average value

9. Select the Reference incl. in calc. service function

and start the measurement using the Check key.

This help function shows the result of the

calculation of the raw data, dark current data and

reference sensor data. It is used for the error

search during development.

10.Select the Fully incl. in calc service function and

start the measurement using the Check key.

This help function shows the logarithm of the

calculation of the raw data, dark current data and

reference sensor data. It is used for the error

search during development.

11.Press the Next> softkey to switch to the

distribution test.

12.Select the Average value service function and

start the measurement using the Check key.

This help function shows the average value of

several repetitions of a measurement. The

number of repetitions can be preset in the entry

field. It is used for the error search during

development.


English (EN)40

RMS value

Min/max value

Abbreviations• HDR= high dynamic range (high contrast)

• ADC= Xenon flashes

• RMS= root mean square (effective value)

The RMS value of a series of values (or continuous signal) is the square root of the arithmetic mean

(average) of the squares of the original values (or the square of the function that defines the continuous

waveform).

5.5.3.5 "Buzzer" service function

The buzzer emits a signal tone.

13.Select the RMS value service function and start

the measurement using the Check key.

This help function shows the scattering (noise)

after several repetitions of a measurement. The

number of repetitions can be preset in the entry

field. It is used for the error search during

development.

14.Select the Min/max value service function and

start the measurement by pressing the Check

key.

This help function shows the minimum and

maximum measured values after a measurement

has been repeated several times. The number of

repetitions can be preset in the entry field. It is

used for the error search during development.

Select the Buzzer service function and access it


41Troubleshooting


English (EN)

5.5.4 "Software" subgroup

5.5.4.1 "Update" service function

Prerequisites

A prepared USB stick must be available. (see Updating the device software on p. 79)

Switch to the Software subdirectory in the Sub

Groups column.

1. Select the Update service function and access it


The "Update" screen appears.

Follow the instructions shown on the display.


English (EN)42

5.5.4.2 "Reset factory settings" service function

5.5.4.3 "Backup" or "Restore" service function

1. Select the Reset factory settings service function

and access it using the Enter key.

2. Press the Yes softkey to reset the factory settings.

This will not delete the calibration data

(temperature alignment).

3. Follow the instructions on the display.

The No softkey cancels the process.

1. Select the Backup/Restore service function and

access it using the Enter key.

2. Select data backup using the Backup softkey or

restore the data using the Restore softkey.

3. Follow the instructions in the information field.

4. Start the backup process using the Backup

softkey.

5. Start restoring the data using the Restore softkey.

43Troubleshooting


English (EN)

5.5.5 "Information" subgroup

5.5.5.1 "Device information" service function

After replacing the printed circuit board or Xenon lamp, the corresponding data must be corrected at this

location.

Entry of serial number and number of lamp flashes after replacing the printed circuit board

Switch to the Information subdirectory in the Sub

Groups column.

Note the displayed number of lamp flashes before replacing the printed circuit board.

Select the Device information customer service

function and access it using the Enter key.

The serial number of the device is located on the name plate. The name plate is located on the

rear side of the device.

The numeric keys are allocated with both letters and digits. Press the numeric keys until the

required letter or number is marked. The selection appear in the display field after a short

period of time.

Enter the serial number of the device and the

noted number of lamp flashes in the

corresponding fields.


English (EN)44

Resetting the number of lamp flashes

Prerequisites

The Xenon lamp has been replaced.

Exporting device information

Switch to the lamp flash display.

Press the zero numeric key.

Press the Save softkey to save the device

information in the BioSpectrometer.

The data will be saved and the function menu will

then be shown in the display.

Press the Export key to start the export process.

Observe the instructions in the information window.

Select the storage medium and confirm it using

the Enter key.

Press the Export softkey to save the device

information.

The device information will be written to a file

named "BioSpectrometerDeviceInfo.txt" and

saved on the selected medium.

The Cancel softkey cancels the export process.

45Troubleshooting


English (EN)

5.5.5.2 "Logfile" service function

Exporting the logfile

5.5.5.3 "Self test result" service function

1. Select the Self test result service function and access it using the Enter key.

The results of the self test routines are displayed.

Select the Logfile service function and access it


Press Page dn softkey to see the next page.

Press Page up softkey to see the previous page.

Press the Export key to start the export process.

Observe the instructions in the information window.

Select the storage medium and confirm it using

the Enter key.

Press the Export softkey to save the logfile.

The logfile will be written to a file named

"user.log" and saved on the selected medium.

The content of this file must be displayed using a

word processing program, e.g., WordPad.

The Cancel softkey cancels the export process.


English (EN)46

5.5.6 "Adjustment" subgroup

5.5.6.1 "Optical adjustment" service function

Prerequisites

The liquid filter must be tempered before the adjustment to room temperature.

Prerequisites

The cuvette shaft must be empty before the HDR factor measurement.

Switch to the Adjustment subdirectory in the Sub

Groups column.

Observe the instructions in the material safety data sheets for the holmium filter and

didymium filter. The material safety data sheets can be found on the

http://www.eppendorf-support.com website.

Every time it is assembled, the device must be adjusted using the "Optical adjustment" service

function.

1. Select the Optical adjustment customer service


47Troubleshooting


English (EN)

Prerequisites

The cuvette shaft must be empty before the blank measurement.

The USB stick included in the filter set must be connected to the BioSpectrometer.

2. Press the blank key. Wait for the measurement.

3. Press the blank key. Wait for the measurement.

The display view will switch after the

measurement has been completed. In the

information window, you will be prompted to

insert the holmium filter.

4. Insert the holmium filter and press the Sample

key.

The display view will switch after the

measurement has been completed. In the

information window, you will be prompted to

insert the didymium filter.


English (EN)48

5.5.6.2 "Intensity adjustment" service function

Select the Intensity adjustment service function and access it using the Enter key.

The display for optical adjustment appears.

The following description applies to the following devices:

5. Insert the didymium filter and press the Sample

key.

6. Press the OK softkey to end the alignment after

the last measurement.

Device As of serial number

BioSpectrometer basic 6135CL601321

BioSpectrometer kinetic 6136CJ601387

BioSpectrometer fluorescence 6137CJ701337

BioPhotometer D30 6133CL400223

All repaired devices with a new mirror

(6135 866.103).

–

49Troubleshooting


English (EN)

For older devices with the mirror 6135 866.006, the following settings apply:

Intensity• The display continuously shows the light

intensity of the sensors.

• One peak from the spectrum is shown in

addition to the intensity of the reference sensor.

• The upper bar shows the intensity of the current

measurement and the lower bar shows the

maximum intensity since the function was

accessed.

• Peak 2 must have an intensity of at least 29%.

• The spectrum (green) may not exceed the red

saturation line.

• The tip of Peak 2, marked in black, must be on

the green reference line.

Intensity• The display continuously shows the light

intensity of the sensors.

• Two peaks from the spectrum were shown in

addition to the intensity of the reference sensor.

• To guarantee correct operation of the

BioSpectrometer, Peak 1 and the reference

sensor must have an intensity of at least 40%.

• The upper bar shows the intensity of the current

measurement and the lower bar shows the

maximum intensity since the function was

accessed.

• Peak 2 must have an intensity of at least 29%.

• The spectrum (green) may not exceed the red

saturation line.

• The tip of Peak 2, marked in black, must be on

the green reference line.


English (EN)50

5.5.6.3 "Thermal adjustment" service function

This function is only available on the BioSpectrometer kinetic.

For the BioSpectrometer kinetic 6136, use the temperature validation system MC/MC ep

(order no. 0055 000.298) and the corresponding temperature sensor (order no.

6135 870.003).

You will receive additional information for the measurement process in the information

window.

1. Select the Thermal adjustment service function


2. Insert the temperature adapter in the cuvette

shaft.

3. Guide the measurement lead to the front side of

the BioSpectrometer.

4. Connect the temperature sensor to the Testo

thermometer.

5. Select the temperature to be calibrated and

adjusted.

6. Press the Start softkey.

7. Wait for the measurement.

The text "Tempering..." appears during the

measurement; the text switches to "Ready" as

soon as the measurement has been completed.

8. Read off the temperature from the Testo

thermometer, enter it in the selected field and

confirm it using the Enter key.

Press the Abort softkey to exit the function and

return to the function menu.

9. Carry out the adjustment again for the other four

temperatures.

51Troubleshooting


English (EN)

5.5.6.4 "Continuous flashing" service function

This function is to be used, if necessary, to adjust the light path.

5.6 Customer service functions

5.6.1 Navigation

1. Use the up and down arrow keys to select the entries in a column (groups, functions).

2. Use the left and right arrow keys to switch from one column to another.

5.6.2 Activating customer service functions

Proceed as follows to activate the customer service functions:

Prerequisites

Situation 1: The device is switched off.

You want to switch on the device and immediately access the customer service functions.

Continue with Item 3 if you would like to switch from the service functions to the customer service

functions (situation 2).

Select the Continuous flashing service function


The Xenon lamp flashes continuously.


English (EN)52

Prerequisites

Situation 2: You would like to switch from the service functions to customer service functions.

5.6.3 "Device settings" subgroup

1. Switch the BioSpectrometer on.

2. Press the Function softkey.

The display with the customer service functions

appears.

3. Switch to the main directory.

4. Select the User entry.

Switch to the Device settings subdirectory in the

Sub Groups column.

53Troubleshooting


English (EN)

5.6.3.1 "Settings" customer service function

Select language

Prerequisites

The field for the language selection is highlighted in color.

Selecting the self test interval

1. Select the Settings customer service function and

access it using the Enter key.

2. Open the field for the language selection using

the Enter key.

The field becomes a list with several entries.

3. Select the required language from the list.

4. Press the Save softkey to adopt the selection.

5. Then turn the device off and back on to switch the

display texts to the selected language.

Press the Cancel softkey to exit the device

settings and return to the function menu.

6. Select the selection field for the self test interval.

7. Open the selection field using the Enter key.

8. Select the required interval from the list.

9. Press the Save softkey to adopt the selection.

Press the Cancel softkey to exit the device

settings and return to the function menu.

A description of how to correct the date and time can be found in the operating manual.


English (EN)54

5.6.4 "Device calibration" subgroup

5.6.4.1 "Spectrometer unit" customer service function

Check wavelength accuracy

Switch to the Device calibration subdirectory in

the Sub Groups column.

For this function test, you will need the BioSpectrometer customer reference filter set (order

no. 6135 928.001) or service test filter set (order no. 6135 931.002).

1. Select the Spectrometer unit customer service


2. Select the Check wavelength systematic error

function and activate it using the Enter key.

3. Press the Next> softkey.



55Troubleshooting


English (EN)

Check photometric accuracy

Prerequisites

The Spectrometer unit customer service function has been accessed.

4. Insert the A0 blank filter in the cuvette shaft and

press the blank key. Wait for the measurement.

5. Insert the 260 nm test filter in the cuvette shaft

and press the Sample key. Wait for the

measurement.

6. Repeat the measurement with the 280 nm and

800 nm test filters.

The last measurement can be repeated using the

<Back softkey.



After all measurements have been completed, the

measured values for the three wavelengths are

shown in the display.

7. Compare the measured values to the limiting

values in the provided filter set table.

An error analysis must be completed if a

measured value is outside of the range of limiting

values.

8. Press the Finish softkey to return to the

"Spectrometer unit" customer service function.

9. Select the Check photometric accuracy function

and activate it using the Enter key.

10.Press the Next> softkey.




English (EN)56

5.6.4.2 "Temperature unit" customer service function

Prerequisites

The cuvette shaft must be empty.

11.Insert the A0 blank filter in the cuvette shaft and

press the blank key. Wait for calibration to be

completed.

12.Insert the A1 test filter in the cuvette shaft and

press the Sample key. Wait for calibration to be

completed.

13.Repeat calibration with the test filters A2 and A3.

The last measurement can be repeated using the

<Back softkey.



The device measures the test filter 15 times at

nine wavelengths. Average values and variation

coefficients of the measurement series can then

be read in the display.

The list of measured values can be moved using

the arrow keys.

14.Compare the average values and variation

coefficients to the limiting values in the table

provided.

If a measured value is outside of the limiting

values, the error must be analyzed and corrected.

15.Press the Finish softkey to return to the

"Spectrometer unit" customer service function.

1. Select the Temperature unit customer service


2. Start the calibration using the Next> softkey.

The calibration of the five temperatures takes

approx. 30 minutes.

The estimated remaining time is displayed during

calibration.



57Troubleshooting


English (EN)

5.6.4.3 "Temperature unit" customer service function with an external temperature tester

The internal temperature measurement can be tested using an external measuring device.

3. Check the status of the cuvette shaft temperature

control.

An error analysis must be completed if one or

more statuses are marked as "fail".

4. Press the Temperature unit softkey to return to

the "Spectrometer unit" customer service

function.

1. Insert the temperature measuring adapter in the

cuvette shaft.

2. Guide the measurement lead to the front side of

the BioSpectrometer.

3. Connect the temperature measuring adapter to

the Testo temperature tester.

4. Select the Temperature unit customer service


5. Start the calibration using the Next> softkey.

6. tempering... appears on the display when the

cuvette holder is being heated.

7. During vernier adjustment of the temperature,

checking... appears on the display.


English (EN)58

5.6.4.4 "Fluorescence unit" customer service function

1. Select the Fluorescence unit customer service function and access it using the Enter key.

2. Insert the F1 filter in the cuvette shaft. The label must be on the left side.

4. Compare the ratios and variation coefficients to the limiting values in the table provided.

If a measured value is outside of the limiting values, the error must be analyzed and corrected.

8. The device emits a signal tone when the internal

measured value has reached the set value. The ok

status is also shown in the display.

9. Read off the measured value on the Testo

temperature tester right after the signal tone has

been emitted.

The device continues to heat the cuvette holder

without delay.

10.Repeat the measurement for all displayed

temperatures.

11.Adjust the device if one or more of the measuring

results deviates from the set value by ±0.3°C (see

"Thermal adjustment" service function on p. 50).

For this function test, you will need the fluorescence test filter (order no. 6137 935.005).

3. Start calibration using the Measure softkey.

The device measures the test filter 15 times at 2

emission wavelengths. After the measurement

has been completed, the display shows 2

characteristic values: "Ratio" as the dimension for

the correct adjustment and "CV" as the dimension

for the noise.

Press the OK softkey to exit the function and


59Troubleshooting


English (EN)

5.6.4.5 "Perform self test" customer service function

1. Select the Perform self test customer service function and access it using the Enter key.

The self test automatically elapses and shows the progress in the display.

2. Check the test result.

An error analysis must be completed if one or more of the results are marked as "fail".

3. Press the OK softkey to return to the "Perform self test" customer service function.


English (EN)60

61Disassembly/assembly


English (EN)

6 Disassembly/assembly6.1 Dismantling or replacing components

6.1.1 Required tools

• 1.5 AF Allen screwdriver

• T10, T20, T6 torx screwdriver

• T6 torx screwdriver, angled

• PH1 watchmaker Phillips screwdriver

6.1.2 Disassembling the housing

Abb. 6-1: Device feet (1) and housing screws (2)

Fig. 6-1: Device feet (1) and housing screws (2)

1. Place the BioSpectrometer on a stable surface so the device feet (1) point upward.

2. Remove three device feet from the base plate using a flat head screwdriver. The spherical support of the

spherical foot is anchored in the base plate using locks.

3. Remove the torx screws (2).

Each time it is assembled, the device must be adjusted using the "Optical adjustment" service

function (see "Adjustment" subgroup on p. 46).

BioSpecrometer

1

2

Disassembly/assemblyEppendorf BioSpectrometer® 6135/6136/6137

English (EN)62

4. Reinsert the device feet in the base plate.

5. Place the BioSpectrometer on the device feet.

6. Carefully lift the upper part of housing upward (cable connections) and place it next to the

BioSpectrometer.Abb. 6-2: Setting down the upper part of housing

Fig. 6-2: Setting down the upper part of housing

6.1.3 Replacing the lamp with the lamp holder

Prerequisites

The upper part of the housing has been removed.

Abb. 6-3: Adjustment tool (1) on the lamp holder

Fig. 6-3: Adjustment tool (1) on the lamp holder

23

45

6

78

9

kin

eti

c

Bio

Sp

ect

rom

ete

r

1



English (EN)

1. Screw the adjustment tool (1) for the lamp holder (order no. 6135 868.009) on to the housing using two

screws from the EMC shielding plate.

To lock the position of the lamp holder, the adjustment tool must be placed form-locking on the lamp

holder.

2. Remove the reference diode plug

3. Pos. 2) from the PCB sensor.

4. Carefully remove the reference diode cable from the cable routing.Abb. 6-4: EMC shielding plate (2) on the lamp holder

Fig. 6-4: EMC shielding plate (2) on the lamp holder

5. Unscrew the EMC shielding plate (2) (three T10 torx screws (1)).Abb. 6-5: Lamp holder (1) with connections (2, 3)

Fig. 6-5: Lamp holder (1) with connections (2, 3)

6. Remove the plug (3) for the Xenon lamp power supply from the PCB BioSpectrometer.

7. Unscrew the lamp holder (1) (three T10 torx screws).

8. Replace the old lamp holder with a new one.

9. Place the new lamp holder form-locking on the adjustment tool and screw it down.

10.Connect the Xenon lamp to the power supply on the PCB BioSpectrometer.

11.Insert the reference diode plug in the PCB sensor.

12.Screw down the EMC shielding plate.

13.Attach the upper housing part.

14.Check the intensity using the "Intensity adjustment" service function (see "Intensity adjustment" service

function on p. 48).

2

1

1

23


English (EN)64

15.Screw down the upper part of housing.

16.Carry out the "Optical adjustment" service function (see "Adjustment" subgroup on p. 46).

17.Check the wavelength systematic error and photometric accuracy (see "Spectrometer unit" customer

service function on p. 54).

18.Set the flash counter to zero (see "Device information" service function on p. 43).

6.1.4 Replacing the PCB BioSpectrometer

Prerequisites


1. Unscrew the EMC shielding plate (three T10 torx screws).

2. Remove all plugs from the PCB BioSpectrometer.

3. Remove the screws that are used to fasten the PCB BioSpectrometer (seven T10 torx screws).

4. Replace the old PCB BioSpectrometer with a new PCB BioSpectrometer.

5. Screw down the new PCB BioSpectrometer.

6. Insert all plugs, which were previously removed, in the new PCB BioSpectrometer.Abb. 6-6: Binding together the connecting cables

Fig. 6-6: Binding together the connecting cables

7. Bind together the connecting cables from the switching power supply unit to the PCB BioSpectrometer

with the tie wraps.

8. Screw down the EMC shielding plate.

9. Attach the upper housing part.


function on p. 48).

11.Screw down the upper part of housing.




14.Enter the number of lamp flashes and serial number of the BioSpectrometer (see "Device information"


Note the counter reading of the lamp flash. The counter reading must be saved in the new

PCB BioSpectrometer (see "Device information" service function on p. 43) following the

replacement.



English (EN)

6.1.5 Replacing the switching power supply unit

Prerequisites


1. Remove all plugs from the switching power supply unit (1).Abb. 6-7: Switching power supply unit (1) and holding device

Fig. 6-7: Switching power supply unit (1) and holding device

2. Unscrew two nuts from the spacers.

3. Remove the old switching power supply unit and insert a new switching power supply unit.

4. Tighten the nuts.

5. Insert all plugs that were previously removed in the switching power supply unit.Abb. 6-8: Binding together the connecting cables

Fig. 6-8: Binding together the connecting cables

6. Bind together the connecting cables from the switching power supply unit to the PCB BioSpectrometer

with the tie wraps.

1


English (EN)66

7. Attach and tighten the upper part of housing.

8. Carry out the "Optical adjustment" service function (see "Adjustment" subgroup on p. 46).

9. Check the wavelength systematic error and photometric accuracy (see "Spectrometer unit" customer


6.1.6 Replacing the display

Prerequisites


1. Remove the display cable on the PCB BioSpectrometer.

2. Unscrew the display holder and display from the upper part of housing (three T20 torx screws).

3. Unscrew the display holder from the display (four T10 torx screws).

4. Replace the old display with the new display.

5. Screw down the display holder on the new display.

6. Center the Display and display holder in the housing window and tighten it.


8. Test the display (see "Display" service function on p. 36).


6.1.7 Replacing the keypad

Prerequisites


1. Remove the keypad ribbon cable on the PCB BioSpectrometer.

2. Remove the bonded grounding strip from the upper part of housing.

3. Remove the bonded keypad from the upper part of housing. The keypad is mounted to the upper part of

housing using double-sided adhesive tape.

4. Remove tape residue using a suitable solvent.

5. Use grounding strip to bond the new keypad.

6. Attach the ribbon cable to the PCB Biospectrometer.


8. Test the keypad (see "Display" service function on p. 36).




English (EN)

6.1.8 Dismantling the diffraction grating

Prerequisites


Abb. 6-9: Diffraction grating (3) and holding device

Fig. 6-9: Diffraction grating (3) and holding device

1. Unscrew the light shielding plate (2) above the diffraction grating (3) (two T10 torx screws (1)).

2. Unscrew the diffraction grating (3) from the housing (two T10 torx screws).

3. Replace the old diffraction grating with holder with a new component and fasten it on the housing.

4. Screw down the lighting shielding plate.

5. Check the intensity using the "Intensity adjustment" service function (see "Intensity adjustment" service

function on p. 48).





3

2

1


English (EN)68

6.1.9 Dismantling the cuvette shaft

Prerequisites


Abb. 6-10: Cuvette shaft insulation (1) and cuvette holder (2) with connections

Fig. 6-10: Cuvette shaft insulation (1) and cuvette holder (2) with connections

1. Remove the cuvette shaft insulation (1) (BioSpectrometer kinetic).

2. Remove all cables which lead to the cuvette holder (2).

3. Unscrew the cuvette holder (two T10 torx screws T10).

1

2



English (EN)

Abb. 6-11: Separating the cuvette holder from the photoconductor

Fig. 6-11: Separating the cuvette holder from the photoconductor

4. Lightly lift the cuvette holder and tilt it toward the fiber optic cable. Do not grip the heat sink on the

Kinetic version. Do not grip the fluorescence module on the fluorescence version.

5. Remove the cuvette holder from the fiber optic cable.

6. Replace the old cuvette holder with a new one.

7. Place the cuvette holder at the home position. Do not tighten the screws.Abb. 6-12: Adjust the cuvette holder using the adjustment tool (1)

Fig. 6-12: Adjust the cuvette holder using the adjustment tool (1)

8. Insert the adjustment tool (1) in the cuvette shaft and lock it using the knurled screw.

9. Align the cuvette holder flush on the housing and tighten it.

10.Remove the adjustment tool.

11.Connect cables.


function on p. 48).

13.Attach and tighten the upper part of housing.




16.Kinetic version: check the thermal module (see "Temperature unit" customer service function on p. 56).

17.Fluorescence version: check the fluorescence unit (see "Fluorescence unit" customer service function on

p. 58).

1


English (EN)70

6.1.10 Replacing the fiber optic cable

Prerequisites


1. Unscrew the torx set screw (T6) on the fiber optic cable holder.Abb. 6-13: Cuvette shaft insulation (1) and cuvette holder (2) with connections

Fig. 6-13: Cuvette shaft insulation (1) and cuvette holder (2) with connections

2. Remove the cuvette shaft insulation (1) (BioSpectrometer kinetic).

3. Remove all cables which lead to the cuvette holder (2).

4. Unscrew the cuvette holder (two T10 torx screws T10).

5. Lightly lift the cuvette holder and tilt it toward the fiber optic cable. Do not grip the heat sink on the

Kinetic version.

6. Remove the fiber optic cable from the cuvette holder.

7. Remove the fiber optic cable from the second opposite facing holder.

8. Replace the old fiber optic cable with a new one.

9. Insert the new fiber optic cable in the two holders.

10.Place the cuvette holder at the home position. Do not tighten the screws.

11.Insert the adjustment tool in the cuvette shaft and lock it using the knurled screw.

12.Align and tighten the cuvette holder.

1

2



English (EN)

13.Remove the adjustment tool.

14.Connect cables.


function on p. 48).

16.Attach and tighten the upper part of housing.





English (EN)72

6.1.11 Replacing the PCB photo diode array

Prerequisites


Abb. 6-14: Light shielding plate (2) above the photo diode array and PCB photo diode array (3)

Fig. 6-14: Light shielding plate (2) above the photo diode array and PCB photo diode array (3)

1. Unscrew the light shielding plate (2) above the photo diode array (two T10 torx screws (1)).

2. Remove the cable from the PCB photo diode array (3).

Never loosen the two torx screws (1). Loosening these screws causes a misalignment of the

photo diode array, which can only be corrected by the manufacturer.

1

1

2

3

4



English (EN)

3. Unscrew the PCB photo diode array (two torx T6 screws (4)).

4. Remove the PCB photo diode array toward the rear.

5. Install the new PCB photo diode array, starting by attaching it to the photo diode array.


function on p. 48).





6.1.12 Replacing the photo diode array

Prerequisites

The upper part of housing has been removed and the PCB photo diode array has been dismantled.

1. Unscrew the photo diode array holder (three PH1 Phillips screws).

2. Remove the photo diode array and replace it with a new one.

3. Fasten the new photo diode array.


function on p. 48).





Never loosen the two torx screws (1). Loosening these screws causes a misalignment of the

photo diode array, which can only be corrected by the manufacturer.

1


English (EN)74

6.1.13 Replacing the mirror

Abb. 6-15: Mirror holder (1) and mirror (2)

Fig. 6-15: Mirror holder (1) and mirror (2)

Prerequisites


Abb. 6-16: Handling the tweezers for loosening the mirror holder

Fig. 6-16: Handling the tweezers for loosening the mirror holder

1. Loosen the mirror holder lock using angled tweezers while removing the mirror holder (1) using straight

tweezers (see figure).

2. Remove the old mirror (2) and insert the new mirror.

1

2



English (EN)

3. If the mirror holder has become too damaged during removal, replace the old mirror holder with a new

one.

4. Place the mirror holder on the mirror and lock it.


function on p. 48).





6.1.14 Disassembling the fan

(Only BioSpectrometer kinetic)

Prerequisites


1. Remove the fan plug (X21) from the PCB BioSpectrometer.

2. Unscrew the light shielding plate above the diffraction grating (two T10 torx screws).

3. Remove the retaining plate of the fan on the base of the BioSpectrometer (four PH1 Phillips screws).

4. Replace the old fan with a new one.

5. Screw down the fastening plate of the fan.

6. Check the fan function using the "Temperature unit" customer service function (see "Temperature unit"

customer service function on p. 56).






English (EN)76

77Alignment/adjustment


English (EN)

7 Alignment/adjustment7.1 Adjustment

7.1.1 Required tools

• 1.5 AF Allen screwdriver

• T10, T20, T6 torx screwdriver

• Blackout cloth (http://www.thorlabs.de/NewGroupPage9.cfm?ObjectGroup_ID=190)

7.1.2 Adjusting the diffraction grating

Prerequisites


1. Switch on the device and activate the service functions (see Activating service functions on p. 34).

2. Access the "Intensity adjustment" service function (see "Intensity adjustment" service function on p. 48).

Prerequisites

The light shielding plate has been mounted.

3. Loosen the screws on the diffraction grating (two T10 torx screws).

4. Place the diffraction grating on the stop.

5. Slowly adjust the diffraction grating by alternately tightening the screws until the specifications of the

"Intensity adjustment" service function are met (see "Intensity adjustment" service function on p. 48).

6. Tighten the screws on the diffraction grating.

7. Attach and screw on the upper part of the housing.




Each time the device is assembled, it must be adjusted using the "Optical adjustment" service

function (see "Adjustment" subgroup on p. 46).

Observe the information in the safety data sheets for the holmium and didymium filter

(MSDS). The material safety data sheets can be found on the


To facilitate adjustment when the device is open, the area below the light shielding plate must

be covered with a blackout cloth.

Alignment/adjustmentEppendorf BioSpectrometer® 6135/6136/6137

English (EN)78

7.1.3 Adjusting the fiber-optic cable

The process of adjusting the fiber-optic cable consists of two separate steps which refer to two different

points in the device. The two steps mutually influence each other and must be performed several times on

an alternating basis. Peak 1 and the reference sensor must have an intensity of at least 40%. Peak 2 must

have an intensity of at least 29%.

Prerequisites


1. Switch on the device and activate the service functions (see Activating service functions on p. 34).

2. Access the "Intensity adjustment" service function (see "Intensity adjustment" service function on p. 48).

Step 1

3. Loosen the set screw on the fiber-optic cable holder (T6 torx set screw).

4. Turn the metallic holder of the glass fiber using a pair of tweezers.

The value of peak 1 changes. The value must reach a maximum, but must not exceed the red saturation

line.

5. Tighten the set screw on the fiber-optic cable.

Step 2

6. Loosen the screws on the fiber-optic cable holder (two T10 torx screws).

7. Slowly move the holder to the left and right by moving a screwdriver in the bore of the holder to the left

and right.

The value of peak 1 changes. The value must reach a maximum, but must not exceed the red saturation

line.

8. Tighten the screws on the fiber-optic cable holder.

9. Repeat step 1 and 2 until the value of peak 1 reaches at least 40%.

10.Attach and screw on the upper part of the housing.




79Software


English (EN)

8 Software8.1 Updating the device software

Prerequisites

• USB stick with at least 1 GB of memory

• USB stick is formatted in FAT 32

• Computer with Windows XP or Windows 7 operating system

• Internet access

Downloading the software

1. Create a new folder on the computer and name it as desired.

2. Download the "support_X.X.X.X.zip" file from the www.eppendorf-support.com website to the

computer. Save the "support_X.X.X.X.zip" file in the folder just created on the computer.

Extracting software to the USB stick

3. Insert the USB stick in the computer.

4. Unzip the "support_X.X.X.X.zip" file on the connected USB stick.

5. If files with the same name already exist on the USB stick, these files will be overwritten.

The USB stick contains the following items:• "Eppendorf" folder

• "uImage-eco9g20k" file

6. Remove the USB from the computer.

Update the software on the BioSpectrometer

Prerequisites

The BioSpectrometer is switched off.

7. Connect the USB stick to the BioSpectrometer.

8. Switch on the BioSpectrometer.

The BioSpectrometer starts via the USB stick.

The display shows the software version installed on the device and the software version on the USB

stick.

9. Press the Continue softkey to confirm the software update.

You can cancel the process with the Cancel softkey.

The software installed on the device is replaced by the software from the USB stick.

"Update complete" is displayed when the update is complete.

Completing the update

10.Switch off the BioSpectrometer.

11.Disconnect the USB stick from the BioSpectrometer.

SoftwareEppendorf BioSpectrometer® 6135/6136/6137

English (EN)80

81Diagrams


English (EN)

9 Diagrams9.1 Layout plans

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VERTRAULICH ! Schutzvermerk ISO 16016 beachten !

CONFIDENTIAL ! Copyright reserved ISO 16016

The reproduction, distribution and utilization of this document as well as communication of its contens toothers without authorizationis is prohibited. Offenders will be held liable for the payment of damages.All rights reserved in the event of the grant of a patent, utility model of design.

Weitergabe sowie Vervielfältigung dieses Dokuments, Verwertung und Mitteilung seines Inhalts sind verboten, soweit nicht ausdrücklich gestattet. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte für den Fall der Patent,- Bebrauchsmuster- oder Geschmacksmustereintragung vorbehalten.









DiagramsEppendorf BioSpectrometer® 6135/6136/6137

English (EN)82

9.1.2 BioSpectrometer kinetic

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83Diagrams


English (EN)

9.1.3 BioSpectrometer fluorescence

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DiagramsEppendorf BioSpectrometer® 6135/6136/6137

English (EN)84

85Maintenance


English (EN)

10 Maintenance10.1 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer

for disposal please note the following:

10.2 Shipping the device

10.3 Service schedule

WARNING! Risk to health from contaminated device

1. Observe the notes on the decontamination certificate. You find it as a PDF file on our

website (www.eppendorf.com/decontamination).

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the package.

WARNING! Risk to health from contaminated device

1. Observe the notes on the decontamination certificate. You find it as a PDF file on our

website (www.eppendorf.com/decontamination).

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the package.

NOTICE! Damage as a result of incorrect packing.Eppendorf AG is not liable for damage caused by improper packing.

The device may only be stored and transported in its original packaging.

Transport packaging can be ordered via your Eppendorf distribution partner. The order

number can be found in the spare parts list.

Standard service Group 1 Group 2

BioPhotometer BioSpectrometer

6131 6132 6133 6135 6136 6137

No. To be completed every XX months: 12 12 12 12 12 12

M 01 Cleaning the device in accordance with its

operating manual:

X X X X X X

M 02 Visual inspection of the display X X X X X X

M 03 Keypad X X X X X X

MaintenanceEppendorf BioSpectrometer® 6135/6136/6137

English (EN)86

*Carry out an electrical check every 12 months.

M 04 Stability/housing X X X X X X

M 05 Printer output test X X X X X X

M 06 EDP output test (PC) X X X X X X

M 07 Housing X X X X X X

M 08 Analyzing device data and writing it down/

deleting it if necessary

X X X X X X

M 09 Software update X X X X X X

M 10 Carry out dsDNA method using 260 nm test

filter

X X X X X X

Average time consumption: Up to 60 minutes

Operational qualificationWith electrical check*

Group 1 Group 2


6131 6132 6133 6135 6136 6137

X X X X X X

To be completed every XX months: 12 12 12 12 12 12

Average time consumption: Up to 40 minutes Up to 75 minutes

Extra service Group 1 Group 2


6131 6132 6133 6135 6136 6137

To be completed every XX months: 24 24 24 24 24 24

ME 01 Visual inspection after opening the photometer X X X X X X


ME 02 Setting the number of flashes X X


ME 03 Check the wavelength systematic error and

photometric accuracy

X X X X X X


ME 04 Carry out fluorescence test X


ME 05 Carry out kinetic test X


ME 06 Electrical check* X X X X X X


87Maintenance


English (EN)

10.4 Description of service

Tab. 10-1: Standard service

M 01 Cleaning the device in accordance with its operating manualClean the BioSpectrometer as described in the corresponding operating manual and supplement

sheets.

M 02 Visual inspection of the displayIn particular, observe the following items:

• Defective pixels

• Background lighting

• Contrast

• Marginal sharpness

M 03 Key pad

Check the key pad using the corresponding service program and press all the keys.

• Keystroke

• Rebound

See: Service manual description 5.5.3.1 – "Key pad test" service function.

M 04 StabilityChecking the location surface:

• The surface must be level in order to guarantee the stability.

• The BioSpectrometer must be positioned on the working surface.

• All device feet must touch the working surface in order to prevent shifting which can be

caused by incident stray light.

M 05 Printer output testIf available, connect the DPU414 printer and test the printer output.

See: Service manual description 5.6.4.1 Customer service function "Spectrometer unit".

M 06 USB memory stick output testConnect the USB memory stick and test the output.

See: Service manual description 5.5.5.2 – "Log file" service function.

M 07 HousingClean the housing as described in the corresponding operating manual and supplement sheets.

Pay attention to damage that may compromise safety.

M 08 Analyzing device data and writing it down/deleting it if necessaryShow the following data along with the corresponding service program in the display:

• Check the date and time and set them, if necessary.

See: Service manual description 5.5.5.1 – "Device Information" service function.

M 09 Software updateTo use program enhancements, a software update is available free of charge within the scope of

service.

The USB interface to carry out a software update.

The latest software versions can be downloaded at the www.eppendorf-support.com website.

See SM description, Chapter 8: Software.

M 10 Carry out dsDNA method using 260 nm test filterThe result of the method must be within the limiting values of the UV/VIS test filter set of the

260 nm filter.

See user manual Chapter 6.1: Method selection.

MaintenanceEppendorf BioSpectrometer® 6135/6136/6137

English (EN)88

Tab. 10-2: Extra service features

ME 01 Visual inspection after opening the BioSpectrometerIn particular, observe the following items:

• Is there any liquid in the device or in the cuvette shaft?

• Clean or replace the affected parts.

See SM description, Chapter 6.1.2 – "Dismantling the housing".

ME 02 Carry out pixel wavelength assignmentSee SM description, Chapter 5.5.6.1 – "Optical adjustment" service function.

ME 03 Carry out SpectrometertestSee "SM description 5.6.4.1 "Spectrometer unit" customer service function.

ME 04 Carry out fluorescence testSee SM description, Chapter 5.6.4.4 – "Fluorescence unit" customer service function.

ME 05 Carry out kinetic testSee SM description, Chapter 5.6.4.2 – "Temperature unit" customer service function.

ME 06 Electrical checkSee "SOP description, Chapter 3.4 "Electrical check".

89Technical data


English (EN)

11 Technical data11.1 Power supply

11.2 Ambient conditions

Do not expose to direct sunlight.

Power supply 100 V to 240 V ±10 %, 50 Hz to 60 Hz

Overvoltage category II

Degree of pollution 2

Power consumption Maximum power consumption according to name plate: 25 W

Approx. 15 W during operation

Approx. 5 W with the display dimmed



Approx. 5 W with the display dimmed and temperature control switched off



Approx. 5 W with the display dimmed

Permitted mains interruption Approx. 10 ms at 90 V

Approx. 20 ms at 230 V

Protection class I

Fuses T 2.5 A/250 V, 5 mm × 20 mm (2 pcs.)

Operation Ambient temperature: 15°C to 35°C

Rel. humidity: 25% to 70%

Air pressure: 86 kPa to 106 kPa

Air pressure Use up to an altitude of 2000 m above MSL

Technical dataEppendorf BioSpectrometer® 6135/6136/6137

English (EN)90

11.3 Weight/dimensions

11.4 Photometric properties

Weight 5 kg

Dimensions Width: 295 mm

Depth: 400 mm

Height:150 mm

Space required Width: 500 mm (with Eppendorf thermal printer: 750 mm)

Depth: 500 mm

Weight 5.3 kg


Depth: 400 mm

Height:150 mm


Depth: 500 mm

Weight 5.4 kg


Depth: 400 mm

Height:150 mm


Depth: 500 mm

Measuring principle Single beam absorption spectrophotometer with reference beam

Light source Xenon flash lamp

Monochromator Holographic aberration-corrected concave grating

Beam receiver CMOS photodiode array

Wavelengths 200 nm to 830 nm

Wavelength selection Method-dependent, freely selectable

Spectral bandwidth ≤ 4 nm

Smallest step size 1 nm

Systematic wavelength error ±1 nm

Random wavelength error ≤ 0.5 nm

Photometric measuring range 0 A to 3.0 A at 260 nm

Reading accuracy ΔA = 0.001

Random photometric error ≤ 0.002 at A = 0

≤ 0.005 (0.5 %) at A = 1

Systematic photometric error ±1 % at A = 1

Stray light component < 0.05 %

91Technical data


English (EN)

11.5 Incubation

11.6 Further technical parameters

Adjustable temperature range 20°C to 42°C

Smallest step size 0.1°C

Systematic temperature error ±0.2 °C at 25 °C to 37 °C

Random temperature error ±0.15 °C at 25 °C to 37 °C

Cuvette material For measurements in the UV:

Quartz glass or UV transparent plastic (Eppendorf UVette, 220 nm to

1600 nm)

For measurements in the visible range:

Glass or plastic material

Cuvette shaft 12.5 mm × 12.5 mm, untempered

Cuvette shaft 12.5 mm × 12.5 mm, tempered

Overall cuvette height Min. 36 mm

Height of the light beam in the

cuvette

8.5 mm

Key pad 22 foil keys

6 foil keys as softkeys

Result output Absorbance, concentration, scan (absorbance wavelength spectrum)

Additional, method-dependent data (ratio, FOI, background

absorbances)

Fluorimetry: RFU, concentration

Display VGA TFT display 5.7”

Operator guidance language English, French, Spanish, Italian, German

Interfaces USB master for USB stick

USB slave for connecting to a PC

Interface for Eppendorf thermal printer: serial RS 232

Connected devices must meet the safety requirements specified in

IEC 60950-1.

Technical dataEppendorf BioSpectrometer® 6135/6136/6137

English (EN)92

11.7 Application parameters

Methods Preprogrammed and freely programmable methods for all measuring

and evaluation procedures:

• Absorbance measurements with one or more wavelengths, scans

• Fluorescence measurements at 520 nm or 560 nm

• Nucleic acids and proteins, OD600, dye methods (parallel

measurement of biomolecule and dye marking)

• Methods with evaluation via factor, standard and standard series

• Dual wavelength procedure with subtraction and division

evaluation

• Kinetic method: End point, two-point, linear regression

Method-dependent evaluation Absorbance, concentration via factor and standard.

RFU, concentration via standard

Concentration via standard series:

• Linear regression

• Nonlinear regression (2nd and 3rd degree polynoms)

• Spline evaluation

• Linear interpolation (point-to-point evaluation)

Absorbance calculations via subtraction and division

Additional data for nucleic acids: Ratios 260/280 and 260/230; molar

concentration, total yield

Additional data for dye methods: FOI (frequency of incorporation,

labeling density)

Scans: zoom, peak evaluation

Kinetics: Subsequent modification of the time window for the

regression evaluation

Method memory >100 method programs

Measured value memory and

calibration memory

Memory for >1 000 results with all data of the results evaluation and

standard evaluation, sample number, sample name, date and used

parameter set of the method program.

(The number of saved results depends on the number of saved

methods.)

93Ordering information


English (EN)

12 Ordering informationAbb. 12-1: BioSpectrometer with closed housing

Fig. 12-1: BioSpectrometer with closed housing

4ghi

/ 2abc

3def1

jkl5 6

mno

pgrs7 8

tuv9wxyz

0µ %

method

function_

exit

delete

enterstandard blank sample

BioSpectrometer

kinetic

1-3 4-2

Ordering informationEppendorf BioSpectrometer® 6135/6136/6137

English (EN)94

Abb. 12-2: BioSpectrometer view from below

Fig. 12-2: BioSpectrometer view from below

BioSpectrometer

4-2 2-2 1-4 1-5



English (EN)

Abb. 12-3: BioSpectrometer upper part of housing

Fig. 12-3: BioSpectrometer upper part of housing

BioSpectrometer

1-2


English (EN)96

Abb. 12-4: BioSpectrometer with dismantled housing

Fig. 12-4: BioSpectrometer with dismantled housing

3-2

3-7

3-5

3-3

3-1

2-4

2-1

1-1



English (EN)

Abb. 12-5: BioSpectrometer with dismantled housing

Fig. 12-5: BioSpectrometer with dismantled housing

3-2

3-3

3-5

3-7

2-3

3-1

3-8


English (EN)98

Abb. 12-6: BioSpectrometer keypad

Fig. 12-6: BioSpectrometer keypad

Abb. 12-7: BioSpectrometer fluorescence cuvette holder

Fig. 12-7: BioSpectrometer fluorescence cuvette holder

1-3

3-5



English (EN)

Abb. 12-8: BioSpectrometer kinetic cuvette holder

Fig. 12-8: BioSpectrometer kinetic cuvette holder

Abb. 12-9: BioSpectrometer basic cuvette holder

Fig. 12-9: BioSpectrometer basic cuvette holder

3-5

3-5


English (EN)100

Abb. 12-10: BioSpectrometer fiber optic cable

Fig. 12-10: BioSpectrometer fiber optic cable

Abb. 12-11: Cuvette shaft adjustment tool

Fig. 12-11: Cuvette shaft adjustment tool

3-7

5-5



English (EN)

Abb. 12-12: Lamp adjustment tool

Fig. 12-12: Lamp adjustment tool

12.1 Spare parts

12.1.1 Housing

Fig. Item Order no. (International)

Description

— 1-1 6135 852.005 Mains input module12-3 1-2 6135 854.008 Display cpl., incl. Cable

Keypad12-1 1-3 6135 861.004 6135 basic

12-1 1-3 6136 861.008 6136 kinetic

12-1 1-3 6137 861.001 6137 fluorescence

Spherical foot12-2 1-4 5355 863.001 set of 4

Spherical support12-2 1-5 5353 861.001 dark, set of 4

- 1-6 6135 873.002 Upper part of housing without keypad

5-1


English (EN)102

12.1.2 Electrical/electronic equipment

12.1.3 Light path

12.1.4 Accessories


Description

12-4 2-1 6135 853.001 Switched power supply unitFan

12-2 2-2 6136 852.009 DC

12-5 2-3 6135 855.004 PCB sensor12-4 2-4 6136 859.003 PCB BioSpectrometer


Description

12-4 3-1 6135 865.107 Diffraction grating with holder, incl. mirror12-4 3-2 6135 866.103 Mirrow12-4 3-3 6135 864.003 Lampholder cpl., pre-adjusted

incl. reference diode

Cuvette shaft insulation— 3-4 6135 869.005 basic / kinetic

Cuvette holder12-8 3-5 6135 851.009 basic

12-7 3-5 6136 851.002 kinetic

12-12 3-5 6137 851.006 fluorescence

12-9 3-7 6135 856.000 Fiber-optic cable12-5 3-8 6135 857.104 Photo diode array, incl. mirror


Description

— 4-1 6135 867.002 PackingCuvette shaft cover

12-1 4-2 6135 872.006 6135 basic / kinetic

12-1 4-2 6137 872.003 6137 fluorescence



English (EN)

12.1.5 Auxiliary aids


Description

Adjustment tool12-10 5-1 6135 868.009 lamp

12-11 5-5 6135 871.000 cuvette shaft

— 5-2 6135 931.002 Test filter kitTest filter

— 5-3 6137 935.005 fluorescence

— 5-4 5075 851.563 RS 232 DummyCertification test filter set

— 5-6 6137 935.102 fluorescence

— 5-7 6135 931.100 Certification test filter kitTemperature sensor

— 5-8 6135 870.003 for BioSpectrometer kinetic

Add the holmium filter and didymium filter material safety data sheets to the test filter kit

(art. no. 6135 931.002). The material safety data sheets can be found in the product area of the


Always use the test filter kit (art. no. 6135 931.002) in the original packaging.


English (EN)104

13 Technical information

Evaluate your manual

Give us your feedback.

www.eppendorf.com/manualfeedback

Your local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hamburg · Germany

[email protected] · www.eppendorf.com

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