Introduction of institution:The first comprehensive cancer

Center in Pakistan but a center

Where all patients receive multi-

-disciplinary treatment. SKMCH

has established itself as a center

Of excellence. It was founded by

Imran khan in 1994.

Anam yousaf

(2010-am-006)

Content list

Blood bank

Microbiology

Molecular biology

Cytogenetic

Histopathology

Cytopathology

Hematology

Blood bank section:

Blood donation area • Blood collection• Plateletpheresis

Laboratory area• ABO Blood grouping• Cross matching• RPR test• Cell separator

Microbiology section:

• Urine analysis• Culture & sensitivity• Culture reading

• Biochemical tests• Staining• Kit based tests • (for stool samples)• API• Bactec 9240

Molecular biology section:PCR section

• Extraction & amplification

• FISH for HER-2/neu gene

Genotyping section

• Genotyping of HCV

RNA extraction

PCR

Linear Array HCV genotyping detection kit

Cytogenetics section:

Cytogenetic for acquired disorders

samples:

• Blood

• Bone marrow

Processing:

• Cell counting & culturing

• Harvesting & staining

• Cytovision system

Histopathology section:

Grossing of different body tissues

Core & mucosal biopsies

Processing in Leica peloris processing unit

Embedding in Leica EG

Sectioning

Slide preparation & staining

H & E staining

Sectioning by

microtome

Processing(embedd

ing)

grossing

Cytopathology section:• Samples: Staining:

• CSF Giemsa staining

• FNAs Rapi staining

• Body fluids PAP staining

• Sputum Reporting:

• Blood Microscopy

• Urine

• Semen analysis

Hematology section:blood & CSF samples

• CBC + auto differential

• Manual differential by slide

• Cell counts of cells

• Coagulation factors

• ESR

• Flow cytometry for peripheral blood

Bone marrow screening:

Four types of slides:

Bone marrow aspirate

Touch preparation

Peripheral blood

Trephine biopsy

Staining:

Giemsa staining

Iron staining

CBC ( Auto diff) & Sysmex coagulation detector

Flow cytometer, Hema tek stainer & ESR ( Western Gren)

Suggestions and future plans

there should be no internship program. Instead of this research

work with thesis will be more productive for the students.

Will continue my studies in sha ALLAH