,ATTACHMENT3,
RECUR SURVEY, INC.
FINAL REFOPT
THE ACUTE TOXICITYOE'ET?i CL<I - TE'.OL+
CT-'/JF"+O~YllJS~R~O~tJS
JUNE 10, 199l
BR9I-'1)ii3H JOG g 91- 083H
499 Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (201) 788-8700 FAX (201) 788-9165
9111140225 911101PDR ADOCK 05000335
, P PDR
RQUFI SURVEY, INC.
Clam-Trol CT-1 Acute Bioassay with. ILHLJEDI."
Data Re irement
Q~tLoo
York Terrell
Stud C leted oMay 24, 1991
Performin LaboratorAqua Survey, Inc
499 Point Breeze RoadFlemington, New Jersey 08822
Labo a o ro'ect D91-089H
1 of 11
CLll 499 Point Breeze Road ~ Flernington. New Jersey 08822 ~ Telephone (908> 788-8700 FAX (908> 788-9165
RECUR SURVEY, INC.
STATEMENT OF NO CONFIDENTIALITY CLAIMS
No claim of confidentiality is made for any informationcontained in this study on the basis of its falling withinthe scope of FIFRA Section 10 (d) (1) (A), (B) or (C) .
BETZ LABORATORIES, INC.4636 Somerton RoadTrevose, PA 19047
r rCompany Agent
atic Toxicolo ist Laborator ana erTitle
Date
Signature
ASI Project Number: 91-089H
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CLll 499 Point Breeze Roost ~ Flernington. New Jersey 08822 ~ Telephone(908) 788-8700 FAX (908> 788-9155
7 ~7 FIQUR SURVEY INC
THE ACUTE TOXICITY BIOASSAY OF CLAM-TROL CT-1
This study meets the requirements for 40 CFR Part 160,however dilution water chemical/physical characteristicswere performed by Nytest Environmental, Inc. and might ormight not meet these requirements.
Submitter:
Signature:
Betz laboratories, Inc.4636 Somerton RoadTrevose, PA 19047
Date
Sponsor:
Signature:
Betz Laboratories, Inc.4636 Somerton RoadTrevose, PA 19047
Date
Study Director:ork Terrell D te
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CL/I rt(rr) Point Breeze Road ~ Flemington, New Jersey 08822 ~ Tetephone (908) 788-8700 FAX (O08) 788.O)85
R(j)UFI SURVCY, INC.
Summary
A sample of Betz Clam-Trol, CT-l, received from BetzLaboratories, Inc., Somerton Road, Trevose, PA 19047 onMarch 21, 1991, was tested for potential acute toxicity toE'" "'"'
""'"'onductedunder static conditions with daily renewal of thetest solutions at nominal concentrations of 0.625, 1.25,2.5, 5.0 and 10.0 ppm.. Concentration validation made beforerenewals at TO and T73 revealed a mean concentration of0.738, 1.411, 2.834, 5.968 and 11.980 ppm whileconcentration validation made after renewals at T23 and T96revealed a mean concentration of 0.490, 1.043, 2.545, 5.091and 11.402 ppm.
After 96 hours mortality ranged from 254 at the 0.625 ppmconcentration to 1004 at the 5.0 and 10.0 ppmconcentrations.
The nominal 96 hour LC50 was 1.24 ppm calculated by theProbit Method.
4 of 11
CLll 499 Point Breeze Road ~ Flemington. New Jersey 08822 ~ Telephone (908) 788 8700 FAX (908) 788 9i65
RECUR SURVCY, INC.
I. Ob)ective
The ob)ective of this study was to determine the acutetoxic effects of Betz CT-1 in an aquatic environmentand to establish the 96 hour median lethalconcentration (LC50) to g. ~b ogius under static dailyrenewal conditions.
II. Test material
Source:
Name:
Density:
4 Solubilityin Water:
Betz Laboratories, Inc.Somerton RoadTrevose, PA 19047
Clam-Trol~, (CT-1)
100
DateReceived: March 21, 1991
AmountReceived: 1 pint
III. Materials and Methods
A. Method
The method employed was a modification of themethod outlined in Methods for Measuring theAcute Toxicity of Effluents to Freshwater andMarine Organisms EPA/600/4-85/013 March 1985.(See Appendix A for the Protocol and Appendix Bfor the SOP).
B. Test Organisms
~seciee
The test species, representing a typical temperatep 4 * rrwwwww e —"
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CLll 4r)P Pornt 8reeze Road ~ Flernington, New Jerzey 08822 ~ Telephone (r)08) 788-8700 FAX(F08) 788-9165
~,
RCj)UR SURVCY, INC.
e e sic o t o
Animals used for this test were mixed adultamphipods. Animals appeared to be healthy and ingood physical condition.
So c cc a o
All animals utilized in this test were fromNorthwestern Aquatic Sciences, Inc. of Newport,Oregon. The animals were acclimated to dilutionwater and test temperature for six days.
C ~ Test System
ce of ''o Wate
Water utilized for this test was filtered (0.45microns) natural seawater obtained from ManasquanRiver Inlet, Manasquan, New Jersey. (See AppendixD-1 for water characteristics.)
e eratur
The test temperature was 15 +/- 34 C
The test vessels were 1 liter, 10 x 14 cm glassbeakers containing 500 ml of test solution at adepth of 7 cm.
o o er o
A 24-hour light/0-hour dark photoperiod withutilized.~oc~Each test chamber contained ten organism in 500 mlof test solution, resulting in a loading factor ofone organism per 50 mls of test solution.
6 of 11
CUI 499 Point Breeze Road ~ Flemington. New Jersey 08822 ~ Telephone (908) 788-8700 FAX (908) 788-9168
RECUR SURVEY, INC.
D. Test Design
A range finding test was conducted separately.Four concentrations of the test material wereevaluated using 5 amphipods per treatment. Thedefinitive test was conducted using 10 organismsin each of 2 replicates at each of five treatmentlevels.
~ao t ol
A control treatment was run concurrently with thetest substance treatments using the same dilutionwater and the same number of organisms as per thetest concentrations.
es 'tiat o
Testing was conducted under static conditions withdaily renewal of the test solutions. An initialtest material stock solution of 1,000 ppm wasprepared with ASI DI water. Test solutions wereprepared from the stock solution and thendispensed into the test chambers. The test wasinitiated when, within 30 minutes of test solutionpreparation, the test organisms were transferredto the test chambers.
The range-finding test spanned 72 hours and wasconducted using 5 organisms at each of fourtreatment levels: 1.0, 10.0, 100 and 1000 ppm.Test results indicated a toxic response between1.0 and 10.0 ppm. Exposure at the lowerconcentration level resulted in 204 mortalityafter 72 hours, while exposure at the higher levelresulted in 1004 mortality after 72 hours.
Results from this test were used to determine thetreatment levels for the definitive test. Theconcentrations used were 0.625, 1.25, 2.5, 5.0 and10.0 ppm. The definitive test was for 96 hours (4days) duration and was conducted using two (2) re-replicates of ten organisms at each of fivetreatment levels.
7 of ll
CVI 499 Point Breeze Road ~ Flemington, New Jersey 08822 ~ Terepnone (908) 788-8700 FAX <908> 788-9168
FlQUFl SURVEY, INC.
Test vessels were examined at each 24 hourinterval from test initiation through testtermination. Survival/mortality determinationswere recorded for each chamber. Dead animals wereremoved and discarded.
C e ica A ses
Concentrations were validated at TO, T23, T73 andT96 using Clam-Trol~, CT-1 methyl orange method(supplied by Betz Lab) in the range of 0.2 — 5.0mg/l. A Bausch 6 Lomb Spectronic 21 was used forthe absorbance readings. (see appendix, page C-l.)
s'c 1 C e ic a ete
The temperature, pH, dissolved oxygen (DO)concentration and salinity were measured andrecorded for each treatment, level at the beginningof the test and at each 24 hour test intervalthereafter.
IV. Results
The definitive test resulted in mortalityranging from 254 at the 0.625 ppm concentration to 100%at the 5 and 10 ppm concentrations (See Table 1).
The test solution temperature was maintained at15.0 C. (See Table II).Dissolved oxygen was maintained between 6.8 and 8.2mg/l. (See Table III).The pH ranges during the test were between 7.7 and 7.8(See Table IV).
~ The mean concentration validation before each renewalperiod at TO and T73, and at the end of each renewalperiod at T23 and T96 can be seen in Table V.
V. Source of Documentation
All original data documentation is being maintained .atAqua Survey, Inc., 499 Point Breeze Road, Flemington,NJ 08822, in Betz Laboratories Notebook 002, pages 81-92.
8 of 11
499 Point Breeze Road ~ Flemington, New Jersey 08822 i Telephone (908) 788-8700 FAX (908) 788-9165
RECUR SURVEY, INC.
Table I:o t D a
ExposureTime ~Co t~o g 6~(hrs. ) A B A B A B A B A B
~0A B
0 0 0 0 00 0 0
0 0 0 0
0 0 0 0 010 0 0 0
0 90 900 0 8 70 00 00
96 10 0 30 0 50 70 0 0
Table IIe e tu ees
ExposureTime ~Co t ol
(hrs.) A B A B A B~50A B A B
~0A B
0 0 0 15 0 15.0 5 0 0 5.0 5 0 5 0 0 5 0 15 0 15.015 0 5.0 15 0 15 0 15.0 5.0 15 0 5 0 5 0 5 0 15.0 15.0
5 5 0 0 5 5 0 .07296
Table III
15 5 0 5 0 0 5 015 0 5 5 0 5 0 15 0 5 0 5 5
Dissol ed 0 e m 1
0 0
ExposureTime ~co t~o ~06 5
(hrs. ) A B A B A B A B A B~0A B
0 0 8 0 8 0 8.0 8 07 8 7 8 7 7 7 8 7 87 7 8 7 8 7 7
0 0 0 0 0 0 0
8 8 8 8 8 8.72 8 1 8 8 896 7 3 7.3 7 0 7 0 7 0 6 9 6 8 6 7
Table IV
ExposureTime ~Cont o
(hrs.) A B0.625A 5 A B
~5A B
~0A B
~00A B
0 0 8 7 8 8 7 8 7 7 7 7 8 87 7 8 8 7 8 7 7 7 7 7 8
8 7 7 8 7 7 8 7 8 7 8 8 7.8 7 7 872 7 8 7 8 7 8 8 7 8 7.8 7 8 7 8 8 7 8
6 7 7 7 7 7 7 7
9 of llCVl 499 Point Breeze Road ~ Flernington, New Jersey 08822 ~ Teiephone (908) 788-8700 FAX (908) 788-9165
RECUR SURVCV, INC.
Table V
a 'o
Conc.ppm
0.625
1.25
2.5
5 '
10.0
0.749
1.305
2.695
5.901
11.980
0.726
1. 516
2.973
6 '34
Mean
0.738
1.411
2.834
5.968
11.980
o ce tration Vali atio fte e ew
Conc.ppm
Mean
0.625
1.25
2.5
5.0
10.0
0.498
1.137
2.528
5.056
11.402
0.482
0.949
2.561
5.126
0 ~ 490
1 '432.545
5 '9111.402
10 of 11
CVI 4ort Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (r708) 788 8700 FAx (o08> 788 oio8
"ONCENTRATZON10.00005.00002.50001.25000.6250
NUMBEREXPOSED
2020202020
NUMBERAFFECTED
202015
95
PERCENTAFFECTED
100.0000000100.000000075.000000045.000000025.0000000
BINOMIALPROBABILITY
(~)0.00009540.00009542.0694730
41.19015002.0694730
METHOD SPAN
RESULTS
EC5095 PERCENT
CONFIDENCE LIMITS
BINOMIAL 1.397710 0.625000 — 2.500000
'DROVING AVERAGE
PROBZT
0.109754
0.130604
1.295583
1.238264
0.927734 — 1.675829
0.903236 — 1.602543
INTERPRETATION OF STATISTICS
The order of preference of results is:BEST: Probit MethodBETTER: Moving AverageGOOD: BinomialGOOD: Graphic Interpolation (Graph drawn by hand)
assuming all are statistically valid.To Determine if a method has given valid results:GRAPHICAL: Always validBINOMIAL: The program will tell you if this is not valid.MOVING AVERAGE: The number of spans needs to be no less than one
lower than the number of concentrations tested(excluding control).
PROBIT: The program will tell you if this is not valid
Page 11 of 11
A
P
P
N
D
I
RECUR SURVe, lac.
A8Z MOc 9100 '7Map 1i < 1991
PROTOCOLACUTE TOXICITY BIOA88AY
POR ~o~s
SPONSOR: 0
6 6 om o o
vo e 0 7
STUDY NUMBER: -0 9
TESTING FACILITY: Aqua Survey, Inc. (AS I)499 Point Breeze RoadFlemington, New Jersey 08822United States of America
PROPOSED START DATE: a 20 9
PROPOSED COMPLETION DATE: a 27 1991
STUDY DIRECTOR
QA OFFICER:
SPONSOR APPROVAL:
Signature)
S inureSignature
Dat'e
f»;Date
Date
1 of 6
499 Potnt 3reeze Hoed t=!en.in@ion,."ew .'-..e'l,::"'"" 'e.- ."„,"e 9Q3)7ac.87CO V X:,9C""1733-~iQ=
?Q. Box 3"059 ~ Ba;on Rouge. iou)5lono:C3r ' <!eon ne (3QQ) I' 4534 FAX ( 04j 92'93'
RECUR SURVqy, igC
EUREOSE
To determine the acute toxicity of the test material in the aquaticdIII I I IY I
conditions for 96 hours.
TEST ARTICLE
The test article is ~ which was received from et bs c. on1YIIILtl' ' (description). The sample islabeled with an ASI number ~~0 . Any test article remaining after thetest is completed will be disposed of according to instructions from thesponsor or returned to the sponsor.
TEST ARTICLE CHARACTERISATION:
Data concerning stability, uniformity, composition and additionalchemical/physical characteristics of the test article will be providedby the sponsor on the Test Substance Form (supplied by ASI). Chemicalanalyses will be the responsibility of the sponsor unless priorarrangements have been made for ASI to assume that responsibility.
CONTROL ARTICLE
None required.
TEST SYSTEM
"Iestuarine/marine amphipod.
JUSTIPICATION
"I " Iare a characteristic system in which the response to toxicologicalagents can be evaluated.
DILUTION RLTER
Whenever possible, natural sea water will be used. If use of naturalseawater is not possible, an alternative dilution water, uncontaminatedand of constant quality, will be used.
SOLVENTS
Whenever possible, the toxicant will be introduced into the testsolution without the use of solvents other than water. If alternativesolvents are necessary, they will be used sparingly, not to exceed 0.5ml/l in a solution.
2 of 6
-'99 P":r! 3reeze Road F'en..ing tan, New .:er ey 8822 i'eiegncne ',908) 788- 37CG ~AX (9C8) 738-'.'>.=
?C. Bcx 8"059 ~ Baton Rcuge Lcuisiana 70884 ~ Telegncne (3GO) 05-'- 'F84 'AX {504) 92-'-'-'3-9
RECUR SURVgP ~gg+
TEST MATERIALS
The technical grade, end-use product or both will be used in this study.The brand name or chemical names and a lot number are used to define atest material. Mixtures are defined by ingredient and percent of eachingredient, where possible.
TEST ORGANISMS'
l p'
dorganisms will be as uniform as possible in age and size. The age ofthe organisms will be ascertained from the supplier along with anydisease treatment schedule.
EXPOSURE CHAMBER
One (1) liter all glass beaker or other appropriate size glass chamberswill be used.
RANGE-FINDING TEST:
If the toxicity of the test substance is not already known, a rangefinding test will be performed to determine the range of concentrationsto be used in the definitive test.
DEFINITIVE TESTi
The purpose of the definitive test is to determine the concentration-response curves and the 48 and 96 hour LC50 values.
A minimum of 20 organisms per concentration vill be exposed to five ormore concentrations of the chemical. Each designated treatment groupvill be exposed to a concentration of toxicant that is at least 504 ofthe next highest concentration.Test organisms will be divided into two chambers per treatment, group (10organisms each). All organisms will be randomly assigned to testvessels.
TEST PROCEDURES
In preparation for the test, the chambers are filled with appropriatevolumes of dilution water. The test substance is introduced into eachchamber. The test is started by randomly introducing organismsacclimated in accordance vith the test design into the test chamberswithin 30 minutes after the addition of the test substance. Organismsin the test chambers are observed periodically during the test, the deadanimals removed and the findings recorded. Dissolved oxygenconcentration, pH, temperature and other water quality characteristicsare measured at specified- intervals in test chambers. The test. solutionwill be renewed daily.
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RECUR SURVe, ig(
ACCLZMATXONc
Organisms vill be acclimated to the dilution vater as long as possible.The organisms will receive a standard diet daily until just prior totesting, at which time feeding will be discontinued. Organisms will notbe fed during test. Test organisms will be observed prior to testingfor signs of disease, stress, physical damage and mortality. Injured,dead and abnormal individuals will be discarded.
If organisms show any of these signs, remedial action will be takenduring the acclimatization period and not during the test phase.
Organisms that have been treated for disease are acceptable for testingprovided they do not exhibit signs of disease during the 48 hours ofpretest. Organisms will not be used if they appear to be diseased orstressed or if more than 34 die during the 48 hours immediately prior totesting.
S ~
TEMPERATURE:
LaaEI1!a "" " +/measured daily in at least one test vessel of each test concentration aslong as organisms are surviving at that level.
CONCENTRATION MEASUREMENT:
Analytical measurements of the test substance will be made for eachconcentration at To, T>, Tz, and T9< of the test by a method valida'tedbefore beginning the test by appropriate laboratory practices.
pie
The pH will be measured in at least one test vessel at the beginning ofthe test and daily thereafter at each toxicant concentration as long asanimals are surviving. at that level.
SALINITY:
The salinity will be measured in at least one test vessel at thebeginning of the test and daily thereafter at each toxicantconcentration as long as animals are surviving at that level.
DZSSOLVED OXYGEN
Dissolved oxygen (DO) will be measured at the beginning of the test anddaily thereafter. Measurements will be taken from each concentration aslong as animals are surviving at that concentration.
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PHOTOPERZODs
A 24 hour light and 0 hour dark photoperiod will be employed.
LOADINQ:
The size of the test vessels will be such that the loading factor is nogreater than 1 organism/10 ml of test solution.
OBSERVATIONS:
The organisms will be observed at. 24, 48, 72, and 96 hours withparticular attention being given to general behavior and mortality.A study is not acceptable if more than 104 of the control organisms dieduring a test.
REPORT:
The following are included in the final report:Test Material - If technical grade product is tested, thesource, batch and exact purity are reported. When end-useproduct is tested, the exact percent of active ingredient andthe type of formulation (i.e. granular, wettable powder) arereported.
2 ~ Dilution Rater — Dilution wate source, its chemical/physicalcharacteristics, and pretreatment are reported.
3 ~ Exposure Chambers — The volume, diluent depth, and containerconstruction material are reported.
4 ~ Test Organisms — Source, quarantine, holding and acclimatization,inclusive of feeding schedules and disease treatment procedures,are reported. The age and scientific names are reported.Range Pinding Test — Procedures and results are reported for therange finding study. Included at a minimum are sample size,concentrations tested and mortality data.
6 ~ Definitive Test - Procedures used to prepare toxicant stocksolutions are described. The method of dosing is reported. Thecriteria for determining effects are defined. -Raw data orpercentage of deaths/effects at each treatment level along with thenumber of organisms exposed at each level is reported. Toxicsymptoms, both physical and behavioral, observed during the testare report:ed.
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RECUR SURVqy, igg
7. Calculato4 LCSO Value - LC50 values (48 and 96 hours if possible)with the corresponding 954 confidence intervals and slopes of theconcentration response lines and method of calculation will bereported.
8. Temperature/DO/pH/salinity - Temperature, dissolved oxygen, pH andsalinity measurements are reported along with the range and mean oftemperature.
9. Chemical Analyses - When chemical analyses are made to validatetoxicant concentrations, the analytical method is reported as wellas the results. Residue observed in exposure chambexs isdescribed in the report.
10 'esting Protocols — This protocol is referenced in the report.11. Deviations - Deviations fxom protocol or standard operating
procedures are reported.
2.2. Certification - The Study Directox and the QA Manager certify thefinal report.
RECORDS'll
raw data, data manipulations, draft reports and final reports willbe retained by Aqua Survey, Inc. for a period of five years from thedate of completion of testing. Records will be surrendered to thesponsor upon their written request.
QUALXTY ASSURANCE:
This study will be conducted in accordance with the EPA Good LaboratoryPractice Regulations (Federal Register, Volume 48, November 29, 1983)and Methods .for Measuring the Acute Toxicity of Effluents to Freshwaterand Marine Organisms EPA/600/4-85/013 March 1985,.
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STAHDARD OPERATZNQ PROCEDURE
Acute Toxicity Bioassayfor Amphipods (Daily renewal)
Marino and Estuarine
The objective of this is to determine the acute toxicity of thetest article in the aquatic environment to Marine and EstuarineAmphipods under static daily renewal conditions for 96 hours.
1. Sufficient volume of filtered (0.45 microns) Manasquan seawater properly prepared and stored;
2. Approximately 200 amphipods as uniform as possible in ageand size;
3 ~ 20 1000 ml all glass beakers or other appropriate size glasschambers;
Appropriate size chiller and chiller bath or otherchiller unit;5. Appropriate volume of DZ water;
6. One stainless steel 5-inch diameter or other appropriatesize sieve;
7. Several'isposable clipped (5-mm bore) pipettes;8. Data collection forms;
9. Temp mentor;
10 'lass thermometers (graduated 0.5'C);
11. DO meter;
12. pH meter;
13. Salinity refractometer;14. 6 4-liter or other appropriate size mixing chambers;
1 of 3
15. One(1) and 10 ml graduated glass pipettes;16. 2 100-ml or other appropriate size graduated cylinders;17. One 1000-ml or other appropriate size volumetric flask;18. 40 fathoms sea salt; and
19. Analytical balance.
u e
1. Test Article Preparation
a. Stock solutionFill volumetric flask approximately half full with DZwater. Measure or weigh out appropriate amount of testarticle using glass pipettes or analytical balance.Place test article in volumetric flask and bring up tovolume with DZ water. Place stir bar in flask andplace on star plate for approximately 5 minutes oruntil sample is thoroughly mixed. The stock solutionis usually 1,000 or 10,000 ppm.
b. Test solutionDilution water (filtered Manasquan sea water) should beadjusted to the appropriate salinity with DZ water and40 fathoms sea salt if needed. The temperature shouldbe adjusted by refrigeration or water bath.Appropriate volumes of dilution are then measured usinggraduated cylinders and placed in mixing chambers whereappropriate volumes of stock solution are added andplaced on a stir plate and allowed to thoroughly mix.Appropriate volumes of the test solutions are thenpoured into the test chambers (labelled according toconcentration and replicate and pre-rinsed with theappropriate test solution) and placed into the waterbath.
2 ~ Range-Finding Test
Xf the toxicity of the test article is not known, a range-finding test will be performed to determine the range ofconcentrations to be used in the definitive test. Testconcentrations used for the range finding test are usually.1, 1, 10, 100 and 1,000 ppm, using one replicate of 5organisms each.
2 of 3
3. Definitive Test
All organisms are randomly assigned to test vessels.Carefully pour sand with organisms (acclimated according toprotocol) on to a stainless steel sieve and placed in avessel of dilution water. Allow the sand to filter throughthe sieve, leaving the amphipods in the sieve suspended inthe water. Organisms are then placed two in each testvessel using a clipped pipette until each vessel contains 10organisms.
4. Daily Reading
All readings are taken in accordance with the protocol fromthe mixing chambers prior to being placed in the testchambers with the exception of the final reading which istaken from the test chambers.
6.
Feeding
None.
Daily renewals4
Test chambers are carefully cleared of all detritus and deadorganisms using a disposable pipette. Each chamber is thengently decarted into a clean chamber until approximately 100ml of test solution remains in the chamber, taking care notto pour off any of the test organisms.
Survival/mortality counts and behavioral and morphologicalobservations are made at. this time or in accordance withprotocols.
Appropriate amounts of new test solutions are carefullypoured into the exposure chambers so that the solution runsdown the inner chamber walls.
7 ~ Reporting
All test data will be reported in accordance withrequirements in the protocol.
3 of 3
CLAM-TROL CT-1METHYLORANGE METHODAPPARATUS REQUIRED
CodeCylinder, graduated, 100 mL 121
Cylinder, graduated, 250 mL
Flask, volumetric, 1 L, glass (4 required) 935
Plpet, glass, graduated, 1 mL 140
Plpet, glass, volumetric, 1 mL 888
Pipet, glass, volumetric, 3 mL OO
Pipet, glass, volumetric, 5 mL 124
Plpet, glass, volumetric, 10 mL 123
Pipet, glass, volumetric, 15 mL 881
Pipet, glass, volumetric, 20 mL
Pipet, glass, volumetric, 25 mL 117
Plpet, glass, volumetric, 30 mL
The general apparatus required for the test ls deter-mined by the specific test procedure used.
Apparatus not available through Betz Lab Supplyshould be obtained through a local supplier.
917
CHEMICALS REQUIRED
1, 2- Dichloroethane (reagent)grade or equivalent)
Code 1668
Beaker, glass, 50 mL (2 required) Code
Cylinder, graduated, 25 mL 2822
Funnel Rack, separatory 938
Funnel, separatory, with a Teflon stopcock, 250 mL ~~
(2 required)
Glass Rod 114
Optical Cell, (2 required)
Spectrophotometer
GENERAL APPARATUS ~
CT-1 Buffer Reagent
Methanol (reagent grade or equivalent)
Drying Reagent, with a plastic dipper
SUMMARYOF METHOD
1591
1271
GENERAL PROCEDURE
Use a well-ventilated or hooded area to run the test.Always use a safety bulb when plpettlng Ilqulds.
1,2- Dlchloroethane (also known as Ethylene Dichlo-ride) is a priority pollutant and a specifically-listedRCRA~egulatedmaterial subject to specific disposal re-strictions and/or prohibitions. For this reason, all used1,2- dichloroethane should be segregated from otherwaste streams. Dispose ofwaste 1,2- dlchloroethane inan approved manner (e.g., labpacklng or incineration).
In this procedure the dye in the CT-1 Buffer Reagent com-plexes with the active ingredients in Clam-Trol CT-1. Thiscomplex is extracted into 1, 2- dlchloroethane. The or-ganic layer containing the complex is separated from theaqueous layer and driedwith a drying reagent containinganhydrous sodium sulfate. The color intensity of the 1, 2dichloroethane layer is then measured in a spectropho-tometer at 415 nm.
This method must be customized to each specific appli-cation. Vary the volumes of sample, CT-1 BufferReagent,and 1. 2 - dlchloroethane according to the test range(see Table 1). Ifa higher absorbance is needed, increasethe volume of sample or decrease the volume of 1, 2-olcnioroethane. When increasing the sample volume itmay be necessary to increase the volume of CT-1 BufferReagent used. For samples <150 mL use 10 mL of CT-1Buffer Reagent; for samples between 150 and 300 mLuse 15 mL of CT-1 Buffer Reagent. Make sure thatenough 1, 2 - dlchloroethane is used to leave a smallplug of solvent in the separatory funnel when the bottomlayer of solvent is removed and to fill the optical cellproperly.
AP Ma 9003 ~1990 SETZ LAQORATORIES, INC. ALLRIGHTS RESERVED.C-1
Page t
Ihble 1. Suggestod Votumoa for Various Rangoe of CT-1
RangeCT-1 (mg/L)
0.2- 3.0
1.0 - 25.0
0.2- 1.0
VolumeCT-1 Buffer
(mL)
15
10
15
VolumeDlchloroethane
(mL)
10
VolumeSample
(mL)
OpticalCell Size
1.0 cm
2.5 cm»«
50cm *»"
«The1.0-cm cell (Code 1312) can be used with Hach spectrophotometers using a 1-cm cell adapter (Code 2776C).
*"The 2.&an cell ls the standard Hach 1-in. cell (Betz Code 2601).
««» Five centimeter cells are not available for use with the Hach photometers. Many laboratory spectrophotometersrequire an adapter to accommodate &cm cells. Check with the instrument manufacturer.
1. Transfer an aliquot of the water sample to a separa-tory funnel (the sample). Transfer the same volumeof distilled (or deionized) water to a second separa-tory funnel (the blank). Run the blank once for eachset of samples tested (see Notes 1, 2, and 3).
2. Add CT-1 Buffer Reagent to both the sample and theblank.
3. Using a plpet, add 1, 2- dlchloroethane to both se-paratory funnels.
4. Insert the stoppers in each ofthe separatoryfunrels.Invert and briefly open the stopcock to vent the fun-nels (see Notes 4 and 5). When venting the funnels,point the tip of the funnel away from yourself andothers.
5. Shake the funnels moderately for 30 sec, vent thefunnels, then allow them to stand for 10 mfn (but nolonger than 15 min) ~
6. Collect the lower layer (1, 2 - dlchloroethane) fromeach funnel in 5&mLbeakers leaving about 1-2 mLin the funnel. Th!s willprevent significant removal ofwater.
7. Using the plastic dipper, add 2 scoops of Drying Re-agent to each beaker and stir with a glass rod for 15sec (but no longer than 30 sec).
8. Wait approximately 1 to 2 min (but not more than 5min). Then carefully decant the extract offof the dry-ing reagent into an optical cell.
9. Set the spectrophotometer at 415 nm and zero with1, 2-dlchloroethane. Measure andrecord the absor-bance of the blank and the sample (see Note 6).
10. The sample absorbance minus the blank absor-bance is used to determine the concentration ofCT-1 in the sample. From a prepared calibrationcutve, determine the CT-1 concentration in the sam-ple (see Calibration Curve Preparation).
11. Clean the cells after each measurement(see Note 7).
CALIBRATIONCURVE PREPARATION
1. Prepare a 1000 mg/L CT-1 stock solution by acculrately weighing 1.00 g of CT-ti into 1 L of alstillea (ordeionized) water.
2. Pipet designated volumes of the stock sotution into1-L volumetric flasks. These are the stanaara solu-tions used in preparing a calibration cuive. UseTable 2 to make appropriate dllutlons of the stocksolution for each specific app! Icat ton.
3. Follow the General Procedure using the specific so-lution volumes that have been determinea tor theapplication and prepare a calibration curve, Deter-mine the absorbance of a blank solution using ais-tilled (or deionized) water. This blank can oe sub-tracted from the sample absorbance or usea to zerothe spectrophotometer so that the calibration curvegoes through the origin. The calibration auveshould be linear over the indicated ranges.
NOTES
1. For maximum accuracy the calibration curve snouldbe checked by every operator using this test andshouldbe ver!fied a minimum of twice per mcntnus-ing a freshly prepared CT-1 standard.
2. Ablank measurement must be recorded for earn setof samples. The blank reading may vary siigntly;however, the absolute difference between tne sarn-ple and the blank remains relatively constant.
3. Chlorine causes a negative interference in the test.This can be eliminated by adding 0.1 N SoaiumThiosulfate (Code 235) to the water sample beforerunning the test. The amount added be is based onthe concentration of chlorine in the system. For a100mL water sample containing 0.3 mg/L chlorine,add 10 drops of O.l N Sodium Thiosulfate to removethe Interference.
Page 2C-2
AP 368 9003
~Ie 2. DHutfone for Callbratlon Curve Prepara-tion Based on a En'olutfon Volumeof 1L
ConcentrationCT-1 Desired
(mg/L)
CT-1 Stock Solu-tion Added to Make
1 L (mL)
0.2
0,4
0.6
0.8
1.0
5.0
10.0
15.0
20.0
OZ
0.4
0.6
0.8
1.0
5.0
10.0
15.0
20.0
25.0
4. Asllghtemulslonmay formwhenusing natural watersamples. When this happens, vary step 5 of the
pmcedue. Shake the hmneI for30 sec, vent It, thenallow lt to stand for 5 min. Gently Invert the funnelonce then allow the funnel to stand for 5 min.
5. It Is important to vent the separatiy funnel bothbe-fore and after shaking it. Othiwise, a pressure willbuild up in the funnel that can cause the stopper tobe forced out of the top of the ftInnel.
6. Use caution when Insetting or removing the samplecell ln the photometer. The 1, 2- dlchloroethane candamage the call compartment.
7. It ls imperative that the sample cells are kept cleanduring the running of the test. It Is recommendedthatthe cells are cleaned after each measurement usingthe following procedure:
a) Rinse the cell three times with distilled (ordeionized) water.
b) Rinse the cell three times with methanol.
c) Rinse the call three times with 1, 2 - dlchloro-ethane to remove methanol from the cell.
8. This method isbasedupon Wang, L K.; Langly, D.F.Ind. Eng. Chem., Prod. Res. Dev., 1975, 14, 3,210-212.
AP 388 9003 ~1990 aETZ LABORATORIES,INC.ALLRIGHTS RESERVED.C-3
TOTALANALYTICALSERVICES FOR A SAFE ENVIRONMENTI
Project Ko»t 9117LL91
Log in Ko. t 7861
P.O. No. t Pend! ngOete tAprLL 30, 1991
ANALTTICALOATA REPORT
PACKAGE FOR
Aqtte SUrvey Lno»
499 Point Breete Rd.
Flenfngton, NY OBB22
ATTN:
REFt
Jilt) Todd
Lab Meter
SAHPLE
IDENTIF ICATION
LABORATORY
NUHBER
SAHPLE
HATRLX
SEE NEXT PAGE
xv
ME CERTIFY THAT THIS REPORT LS A
TRUE REPORT OF RESULTS OSTALNE0
FROH OUR TESTS OF THIS HATERIAL.
RE)PE LLY SUBHIT EDr
N EHV ONHEHT L L)i
PARAG )(. SHAH, Ph. 0.ORGANIC LAB~ HANAGER OOU HEELEY
ORY OLRECT R
art¹ 73469
7
))sport orl sample(s) furnished by client apprise to sample(s). Report on sample(s) obtained by us applies only to tot sampled. (nformatxxtcontaned harem e not lo be used for reproductton excepl by sceeal permisaon. Sample(s) writ be relined lor thirty days maximum after dale ofreport unless spectficasy rsouested otherwise by client. In the event that there are portions or ~of sample(s) remain(ng alter Nytest hascpm(usted the reouired tests, feytest shaN have the opuon of retumeg such sample(s) to the client at the client's expense.
box 1538 a 60 seaview blvd., port washington, ny 3 <050 a(536) 625-5500D-1
0 C
LABORATORYNUMBER
SAMPLEIDENTIFICATION
TYPE OFSAMPLE
786100178610027861003
LG SALTLG FRESHLP FRESH
WaterWaterWater
D-2
nytest environments
DATA REPORTING QUALIFIERS
U Indicates compound was analyzed for but not detected. Reportthe minimum detection limit for the sample with the U (e.g. 3.0U ) based on necessary concentration dilution actions. (Thisis not necessarily the instrument detection limit.) Thefootnote should read U-Compound was analyzed for but notdetected. The number is the minimum attainable detected 1'mitfor the sample.
Indicates an estimated value. This flag is used e'ther whenestimating a concentration for tentatively identif'edcompounds whe e a 3.:3. response is assumed or when the massspectral data indicates tne presence of a co-pound that meetsthe identification cr'ter'a but the result is less than thespecified detection limit but greate than zero (e.g.: Iflimit of detection is 3.0 ug/1 and a concentration of 3 ug/1 iscalculated, report as 3Z.)
B This flag is used. when the analyte is found in the blankas well as a sample. 3:t 'ndicates possible/probable blankcontamination and warns t.".e data use= to take appropr'ateaction.
This flag ident'fies al'a=geted compounds that were oundabove the metnod detection limits.
NA This flag indicates that "he compounds are not applicable.
A Aldol condensation produc".Note: Data on soil samples expressed on a dry weight basis.
All non-water samples are reported on soil forms. Th'sincludes samples whose matrix is listed as miscellaneous.
The initial and continuing calibration dates and times for thevolatile fraction are listed on the BFB summary forms. The .
initial and continuing calibration dates and times for thesemi-volatile fractions are listed on the DFTPP summary forms.
D-300003
nytest environment@
Report: of Tests
Water Suitability Test
We find as follows:
The sample was analyzed according to Standard Methods forExamination of Water and Wastewater, 14th Ed., page 888.
Sample ZD: LP FRESH
Growth Ratio
1.08
Lab ED No.: 7861003
Remarks:The sample is of acceptable suitability for the use inbacteriological testing. A ratio greater than 3.0 would indica egrowth promoting substances present in the water. A ratio lessthan 0.8 indicates the presence of growth inhibiting substances.
OO000
nytest environmental
REPORT OF ANALYSZS
Log Zn No.: 7861
We find as follows:
Parameter(s) Sample Zdentification
LG SALT LG FRESH LP FRESH Method(7861001) (7861002) (7861003) Blank
pHSalinity, ppt
'.6629 '
NA„,<1
I
Results in mq(1:
Ammonia NitrogenBromideFluorideZodineNitratePhosphateResidual ChlorineSulfide
'ulfate
Total CyanideTotal Suspended Solids
0. 13040.80.576
<1<0.04<0.04<0.1<0 ~ 101910<0 F 01
3
0.22
<0. 1 <0 '
<0.05<1.0<0.02
<1<0.04<0.04<0.1<0.10
<1<0.01
<1
ppt ~ parts per thousand
D-5
00005
nytest environments
REPORT OF ANALYSIS
Log In No.:7861
We find as follows:
Results in mg/1:
Parameter(s) Sample Identification
LG SALT LG FRESH LP FRESH Method(7861001) (7861002) (7861003) Blank
AluminumArsenicBerylliumBoronCadmiumcalciumChromiumCobaltCopperIronLeadMagnesiumManganeseMercuryMolybenumNickelPotassiumSeleniumSilverZincTotal Organ ic Carbon
<0.2<0.010<0.005
3 3<0.005
271<0.010<0.05
<0.025<0.050<0.050
932<0.015<0.00020-16
<0.040348
<0.010<0.010<0.020
20 ' 11.3
<0.010
<0.005
<0.025
<0.050
<0.0002
<0.040
<0.020
<0.2<0.010<0.005
<0-3.<0.005
<1 ~ 0<0. 010<0.05
<0.025<0.050<0.050<1.0
<0.015<0.0002<0.010<0.040<1.0
<0.010<0.010<0.020
<1 '
00006
1STNYTEST EHVIRONHENTAL INC.
TCL PESTICIOE/PCS ORGANICS ANALYSIS OATA SHEET
SAHPLE HATRIX: llATER
CONC. LEVEL: LOM
EXTRACTION OATE: 4/9/91ANALYSIS OATE: 4/18/91
SAHPLE '10
LAS SANPLE 10tOIL FACTOR:
X HOISTVRE:
VG/L
LG SALT
7861001
1.00HA
CHPO 0 CAS Number PEST ICIOE/PCS CCSIPOJHO
1 [ 319-84.62 I 319 85 73 I 3198684 I 58 89 95 i 76-44-S6 I 309-00-27 I 1024.57.38 I 959 98 8
9 I 60 57 1
10 / 72.55-911 I 70 20 8
12 I 33213 65 9
13 I 72 54 814 I 1031-07-815 I 50 29 3
16 ] 5349C-70.5
17 (72.C3.5
18 I 57 74 9
19 I 8001-35-220 I 12674-11.221 I 11104 28 2
22 ) 11141.16.523 i 53C69.21-924 [ 12672.29-625 I 11097.69.1
26 I 11096 82-5
I
[ Alpha.BHC
f Beta-BHC
i Delta.BHC
[ Ganma-BHC(Lindane)
] Heptachlor
( Aldrin[ Heptachlor Epoxide
( Endosulfan I
( Oieldrin) 4,4'-OOE
f Endrin
( Endosulfan IIt 4,4-000
( Endosulfan Sulfate
I C,4'-OOT
) Endrin Ketone
( Hethoxychlor
( ChlordaneToxaphene
( Aroclor-1016
( Aroclor-1221
)Aroclor' 1232 "
)Aroclor-1242
( Aroclor-1ZCS
t Aroclor-125C
[ Aroclor-1260
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
I
0.050 U.
0-050 U.0.050 U.0.050 V.0 050 U.0.050 U.
0.050 U.
0.050 U.0.100 U.0.100 U.0.100 U.0.100 U.0.100 U.0.100 U.
0-100 V.0.100 U.
0.500 U.
0.500 U.
1.000 U.
0.500 U.
0.500 U.
0.500 U.
0.500 U.
0.500 U.
1.000 U.
1.000 U.
D-7 00007
ORGANOPHOSPHOROUS PESTICZDES
Lcg in No.: 7861Sample ZD No.: LG SALTLab ZD No.: 7861001
Parameters (ug/1)Detection Limit
(ug/1) Found
Vapona (Dichlorvos)
Phorate
Diasinon
Naled (Dibron)
Dementon - S
Disulfoton
Ronnel
Chlorpyrifos (Dursban)
Bolstar (Sulpro fos )
Stirophos
Fensu1f othion
Azinphos methyl (Guthion)
0.2
0.2
0.6
0.2
0.2
0 '
0 '
0.3
0 '
2.0
5.0
1 '
ND
ND
ND
ND
Coumaphos 1.5
Malathion 0.3
Parathion 0.3
ND ~ Not Detected
0-8 on008
1OTNYTEST EHVIROHHENTAL INC
TCL PESTICIDE/PCS ORGAHICS ANALYSiS OATA SHEET
SAHPLE HATRIX: HATER
CONC. LEVEL: LOM
EXTRACTIOH OATEt 4/9/91ANALYSIS DATE: 4/19/91
SAHPLE IO:LAS SAHPLE ID:
OIL FACTOR:
I NOISTUREt
UO/L
PBLKI
A040005
1.00NA
CHPO ¹ CAS Nunber PEST ICIOE/PCS CCHPtRIHD
1 (319.84-6
2 ( 319.85-7
I 319 86 8
4 ( 58.89-95 (
76-44.&6 I 309.00-27 I 1024.57 3
8 ( 959-98 8
9 (60-57-1
10(
72-55-911 I
70-20-812 I 33213-65.913 I 72-54-814 (
1031-07.8
15 I50.29-3
16 (53C9C-70.5
17 I 72 43
18 I 57.74-919 I 8001-35-220 I 12674-11-2
21 I 11104-28-2
22 I11141-'16.5
23 ( 53469-21-924 (
12672.29.625 (
11097.69-1
26 (11096-82-5
I
( Alpha BHC
( Beta.BHC
( Oelta.BHC
(aaaea.BHC(Lindane)
( Heptachlor
( Aldrin( Heptachlor Epoxide
( Endosulfan I
( Oieldrin( C,4'-OOE
( Endrin
(Endosultan tt
( C,4-000
(Endosulfan Sulfate
( 4,4'-ODT
( Endrin Ketone
( Hethoxychtor
( Chlordane
( Toxaphene
( Aroclor-1016
( Aroclor-1221
( Aroclor-1232
( Aroctor-1242
( Aroclor-1248
( Aroctor-1254
( Aroclor-1260
0.050 U.0.050 U,
0.050 U.
0.050 U.
0.050 U.
o.oso u.0.050 U. I
O.oSo u.0.100 U.
0.100 U. I
0.100 U. I
0.100 U.
0.100 U. I
0.100 U.
0.100 U. I
o. loo u.o.sao u.o.soo u.1.000 U. I
o.sao u.o.soo u.Q.saa U.o.soo u. (
0.5QQ U.1.000 U. I
1.0oo u.
D-9
