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Front Matter Source: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 13 (Jul. 1, 1989), pp. i-vii Published by: National Academy of Sciences Stable URL: http://www.jstor.org/stable/33991 . Accessed: 02/05/2014 14:30 Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp . JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. . National Academy of Sciences is collaborating with JSTOR to digitize, preserve and extend access to Proceedings of the National Academy of Sciences of the United States of America. http://www.jstor.org This content downloaded from 130.132.123.28 on Fri, 2 May 2014 14:30:48 PM All use subject to JSTOR Terms and Conditions
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Front MatterSource: Proceedings of the National Academy of Sciences of the United States of America,Vol. 86, No. 13 (Jul. 1, 1989), pp. i-viiPublished by: National Academy of SciencesStable URL: http://www.jstor.org/stable/33991 .

Accessed: 02/05/2014 14:30

Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at .http://www.jstor.org/page/info/about/policies/terms.jsp

.JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range ofcontent in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new formsof scholarship. For more information about JSTOR, please contact [email protected].

.

National Academy of Sciences is collaborating with JSTOR to digitize, preserve and extend access toProceedings of the National Academy of Sciences of the United States of America.

http://www.jstor.org

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JULY 1989

VOLUME 86

NUMBER 13

Proceedings OF THE

National Academy of Sciences OF THE UNITED STATES OF AMERICA

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Proceedings OF THE

National Academy of Sciences OF THE UNITED STATES OF AMERICA

Officers of the Academy

FRANK PRESS, President JAMES D. EBERT, Vice President PETER H. RAVEN, Home Secretary WILLIAM E. GORDON, Foreign Secretary ELKAN R. BLOUT, Treasurer

Editorial Board of the Proceedings

ROBERT H. ABELES GORDON A. BAYM RONALD BRESLOW MICHAEL J. CHAMBERLIN MARY-DELL CHILTON

IGOR B. DAWID, Chairman EDWARD E. DAVID, JR. RONALD L. GRAHAM STUART A. KORNFELD DANIEL E. KOSHLAND, JR. PHILIP W. MAJERUS DANIEL NATHANS

HERBERT E. SCARF MAXINE F. SINGER SOLOMON H. SNYDER HAROLD VARMUS THOMAS A. WALDMANN

Managing Editor: FRANCES R. ZWANZIG Senior Associate Editor: GARY T. COCKS Associate Editor: CAY BUTLER Associate Editor: JOHN M. MALLOY Associate Editor: MARILYN J. MASON Associate Editor: JANET L. MORGAN Associate Editor: T. PEARSON Associate Editor: DOROTHY P. SMITH Associate Editor: COLENE RUCH WALDEN Assistant Managing Editor: JOANNE D'AMICO

Senior Production Editor: LYNN A. WASSYNG Production Editors: BARBARA A. BACON, DEBORAH G. CHLEBOVE, RUTH E. CROSSGROVE,

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Editorial correspondence: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, 2101 Constitution Avenue, Wash- ington, DC 20418.

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Information for Contributors: See p. i (of this issue) and pp. i-viii of issue number 1, January 1989.

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? 1989 by THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.

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Proc. Natl. Acad. Sci. USA Vol. 86, July 1989

CONDENSED INFORMATION FOR CONTRIBUTORS

(See the first issue of the year for the complete Information for Contributors)

The PROCEEDINGS OF THE NATIONAL ACADEMY OF SCI- ENCES USA publishes articles describing original work in the sciences that are of interest to a wide audience. Only mem- bers or foreign associates of the Academy may submit papers, which are accepted on condition that they have not been published and are not submitted elsewhere. Research papers may not exceed five printed pages. Review articles and papers presented at Academy symposia may be pub- lished upon invitation from the Editorial Board.

Submission of Manuscripts. A member may submit up to six manuscripts in any calendar year. For their own papers, members are requested to provide assurance that the manu- script has been reviewed by a knowledgeable colleague who is not a coauthor. A nonmember must submit his/her manu- script to a member who will obtain (at least) two reviews; the member should select the reviewers and they should remain anonymous to the author. In deciding whether to accept a manuscript for publication, the member may overrule a negative review, but the reason(s) why must be explained explicitly on submission of the paper to the PROCEEDINGS office. To ensure that the communicating member is effective in the role of adjudicating differences of opinion between authors and reviewers, it is essential that he/she be expert in the field of the paper, and this must be certified on the transmittal form. The signed reviews, authors' replies, and members' conclusions (where applicable) must accompany the submission. The Editorial Board reserves the right to subject papers to additional review and to make the final decision on acceptance. For further details, consult the complete Information for Contributors [86 (1), pp. i-viii].

The following general conditions apply. (i) Papers reporting experiments involving human subjects or materials must include authorizing documents issued by the appropriate officials from the institution where the work has been done. (ii) Submission of reports involving recombinant DNA as- sumes that National Institutes of Health guidelines, or equiv- alent guidelines in the respective country, have been adhered to. The Editorial Board may request proof to substantiate this assumption. (iii) Publication in the PROCEEDINGS implies that

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oF

Proceedings OF THE

National Academy of Sciences

OF THE UNITED STATES OF AMERICA

July 1989 Volume 86, Number 13 pp. 4803-5216

Table of Contents

CONDENSED INFORMATION FOR CONTRIBUTORS

AUTHOR INDEX ix

Biological Sciences

BIOCHEMISTRY

Infectious potential of human immunodeficiency virus type 1 reverse Brendan A. Larder, Sharon D. Kemp, 4803 transcriptase mutants with altered inhibitor sensitivity and Dorothy J. M. Purifoy

F-actin affinity chromatography: Technique for isolating previously unidentified Kathryn G. Miller and Bruce M. Alberts 4808 actin-binding proteins

Structure of simian immunodeficiency virus regulatory genes Sandra Colombini, Suresh K. Arya, 4813 Marvin S. Reitz, Linda Jagodzinski, Barbara Beaver, and Flossie Wong-Staal

Transient state kinetic evidence for an oligomer in the mechanism of Kinya Otsu, James Kinsella, Bertram 4818 Na'-H' exchange Sacktor, and Jeffrey P. Froehlich

Derivation of an infectious viral RNA by autolytic cleavage of in vitro Aleksandra M. Dzianott and Jozef 4823 transcribed viral cDNAs J. Bujarski

Accessibility of receptor-bound urokinase to type-i plasminogen activator M. Vittoria Cubellis, Peter Andreasen, 4828 inhibitor Pia Ragno, Michael Mayer, Keld

Dan0, and Francesco Blasi

Deletions of bases in one strand of duplex DNA, in contrast to single-base Cheng-Hsilin Hsieh and Jack D. Griffith 4833 mismatches, produce highly kinked molecules: Possible relevance to the folding of single-stranded nucleic acids

* .

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Contents

Rat glucokinase gene: Structure and regulation by insulin Mark A. Magnuson, Teresa L. 4838 Andreone, Richard L. Printz, Steve Koch, and Daryl K. Granner

Purification of a yeast TATA box-binding protein that exhibits human Masami Horikoshi, C. Kathy Wang, 4843 transcription factor IID activity Hiroshi Fujii, James A. Cromlish, P.

Anthony Weil, and Robert G. Roeder

Characterization of a cDNA clone encoding human filaggrin and localization of Lynn J. McKinley-Grant, William M. 4848 the gene to chromosome region 1q21 Idler, Isadore A. Bernstein, David A.

D. Parry, Linda Cannizzaro, Carlo M. Croce, Kay Huebner, Stuart R. Lessin, and Peter M. Steinert

cAMP stimulates transcription of the ,32-adrenergic receptor gene in response Sheila Collins, Michel Bouvier, Mark A. 4853 to short-term agonist exposure Bolanowski, Marc G. Caron, and

Robert J. Lefkowitz

Specific binding of a HeLa cell nuclear protein to RNA sequences in the Richard Gaynor, Emmanuel Soultanakis, 4858 human immunodeficiency virus transactivating region Michio Kuwabara, Joseph Garcia, and

Davis S. Sigman

Serine proteases from nematode and protozoan parasites: Isolation of sequence Judy A. Sakanari, Catherine E. 4863 homologs using generic molecular probes Staunton, Ann E. Eakin, Charles S.

Craik, and James H. McKerrow

Phorbol ester binding to protein kinase C requires a cysteine-rich Yoshitaka Ono, Tomoko Fujii, Koichi 4868 zinc-finger-like sequence Igarashi, Takayoshi Kuno, Chikako

Tanaka, Ushio Kikkawa, and Yasutomi Nishizuka

Identification of critical regions in mouse granulocyte-macrophage Armen B. Shanafelt and Robert A. 4872 colony-stimulating factor by scanning-deletion analysis Kastelein

Studies on the DNA elongation inhibitor and its proliferating cell nuclear Suk-Hee Lee, Ann D. Kwong, Yukio 4877 antigen-dependent control in simian virus 40 DNA replication in vitro Ishimi, and Jerard Hurwitz

Expression of the chicken vimentin gene in transgenic mice: Efficient assembly Yassemi Capetanaki, Steven Starnes, 4882 of the avian protein into the cytoskeleton and Stephanie Smith

Regulation of transcription by cyclic AMP-dependent protein kinase Pamela L. Mellon, Christopher H. Clegg, 4887 Leslay A. Correll, and G. Stanley McKnight

Multiple a subunits of guanine nucleotide-binding proteins in Dictyostelium M. Pupillo, A. Kumagai, G. S. Pitt, 4892 R. A. Firtel, and P. N. Devreotes

Identification of des-(Gly-Ile)-endozepine as an effector of corticotropin- Marc J. Besman, Kazutoshi Yanagibashi, 4897 dependent adrenal steroidogenesis: Stimulation of cholesterol delivery is Terry D. Lee, Masahiro Kawamura, mediated by the peripheral benzodiazepine receptor Peter F. Hall, and John E. Shively

In vitro methylation of Escherichia coli 16S ribosomal RNA and 30S ribosomes Didier N6gre, Carl Weitzmann, and 4902 James Ofengand

cDNA cloning reveals that the major group rhinovirus receptor on HeLa cells Joanne E. Tomassini, Donald Graham, 4907 is intercellular adhesion molecule 1 Corrille M. DeWitt, Donald W.

Lineberger, John A. Rodkey, and Richard J. Colonno

Structural characterization of the rat malic enzyme gene H. Morioka, M. A. Magnuson, T. 4912 Mitsuhashi, M.-K. H. Song, E. Rall, and V. M. Nikodem

cDNA cloning and expression of the human A-type platelet-derived growth Lena Claesson-Welsh, Anders Eriksson, 4917 factor (PDGF) receptor establishes structural similarity to the B-type Bengt Westermark, and Carl- PDGF receptor Henrik Heldin

Expression in bacteria of functional inhibitory subunit of retinal rod cGMP R. Lane Brown and Lubert Stryer 4922 phosphodiesterase

Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects William E. Tapprich, Dixie J. Goss, and 4927 processes involved in the initiation of protein synthesis Albert E. Dahlberg

Iron-sulfur clusters of hydrogenase I and hydrogenase II of Clostridium Michael W. W. Adams, Eric Eccleston, 4932 pasteurianum and James Bryant Howard

Sequence, tissue distribution, and differential expression of mRNA for a Klaus H. Kaestner, Robert J. Christy, 4937 putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes John C. McLenithan, Lelita T. (Clarification) Braiterman, Peter Cornelius, Phillip H.

Pekala, and M. Daniel Lane

iv

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Contents

BIOPHYSICS

On the dynamics of a forced reaction-diffusion model for biological pattern Anastasios A. Tsonis, James B. Elsner, 4938 formation and Panagiotis A. Tsonis

Role of aspartate-96 in proton translocation by bacteriorhodopsin Klaus Gerwert, Benno Hess, Jorg Soppa, 4943 and Dieter Oesterhelt

BOTANY

Specific photoaffinity labeling of two plasma membrane polypeptides with an Glenn R. Hicks, David L. Rayle, Alan 4948 azido auxin M. Jones, and Terri L. Lomax

CELL BIOLOGY

Export of an N-terminal fragment of Escherichia coli flagellin by a Goro Kuwajima, Ikuro Kawagishi, 4953 flagellum-specific pathway Michio Homma, Jun-Ichiro Asaka, Eiji

Kondo, and Robert M. Macnab

Inhibition of human immunodeficiency virus (HIV) replication by Daniel P. Bednarik, Joseph D. Mosca, 4958 HIV-trans-activated a2-interferon N. Babu K. Raj, and Paula M. Pitha

Inhibition of radiation-induced neoplastic transformation by f3-lapachone David A. Boothman and Arthur B. 4963 Pardee

Membranous intermediates in endocytosis are labile, as shown in a Ilona Kessel, Brent D. Holst, and 4968 temperature-sensitive mutant Thomas F. Roth

"Bundle blot" purification and initial protein characterization of hair cell Gordon M. G. Shepherd, Barbara A. 4973 stereocilia Barres, and David P. Corey

Corticotropin positively regulates its own receptors and cAMP rcsponse in Armelle Penhoat, Christine Jaillard, and 4978 cultured bovine adrenal cells Jos6 M. Saez

Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte Jian Kuang, Ji-ying Zhao, David A. 4982 maturation and depletes maturation-promoting activity Wright, Grady F. Saunders, and Potu

N. Rao

Thymidine kinase synthesis is repressed in nonreplicating muscle cells by a Michael K. Gross and Gary F. Merrill 4987 translational mechanism that does not affect the polysomal distribution of thymidine kinase mRNA

EVOLUTION

Modern mammal origins: Evolutionary grades in the Early Cretaceous of North Louis L. Jacobs, Dale A. Winkler, and 4992 America Phillip A. Murry

Gene for the ribulose-1,5-bisphosphate carboxylase small subunit protein of the Barbara A. Boczar, Terrence P. 4996 marine chromophyte Olisthodiscus luteus is similar to that of a Delaney, and Rose Ann Cattolico chemoautotrophic bacterium

Polymorphism for the number of tandemly multiplicated glycerol-3-phosphate Toshiyuki Takano, Shinichi Kusakabe, 5000 dehydrogenase genes in Drosophila melanogaster Akihiko Koga, and Terumi Mukai

GENETICS

Transcriptional bias: A non-Lamarckian mechanism for substrate-induced Bernard D. Davis 5005 mutations

An apaH mutation causes AppppA to accumulate and affects motility and Spencer B. Farr, David N. Arnosti, 5010 catabolite repression in Escherichia coli Michael J. Chamberlin, and

Bruce N. Ames

Plant retrotransposon from Lilium henryi is related to Ty3 of yeast and the D. R. Smyth, Paul Kalitsis, Joan L. 5015 gypsy group of Drosophila Joseph, and John W. Sentry

Structural characterization of the a-glycerol-3-phosphate dehydrogenase- Laurence von Kalm, Jonna Weaver, 5020 encoding gene of Drosophila melanogaster Judy DeMarco, Ross J. Maclntyre,

and David T. Sullivan

Specific chromosomat abnormalities characterize fibrosarcomas of bovine Valerie Lindgren, Maria Sippola-Thiele, 5025 papillomavirus type 1 transgenic mice Jacek Skowronski, Ella Wetzel, Peter

M. Howley, and Douglas Hanahan

Physical mapping of complex genomes by cosmid multiplex analysis G. A. Evans and K. A. Lewis 5030

Linkage mapping of a mouse gene, iv, that controls left-right asymmetry of the Martina Brueckner, Peter D'Eustachio, 5035 heart and viscera and Arthur L. Horwich

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Contents

Involvement of the TCLS gene on human chromosome 1 in T-cell leukemia and Lawrence R. Finger, Jacob Kagan, 5039 melanoma George Christopher, Joanne

Kurtzberg, Michael S. Hershfield, Peter C. Nowell, and Carlo M. Croce

Short, direct repeats at the breakpoints of deletions of the retinoblastoma gene Susan Canning and Thaddeus P. Dryja 5044

Comparison of the chromosomal localization of murine and human Raymond R. O'Neill, Toshiharu Tokoro, 5049 glucocerebrosidase genes and of the deduced amino acid sequences Christine A. Kozak, and

Roscoe 0. Brady

A two-component regulatory system (phoP phoQ) controls Salmonella Samuel I. Miller, Anne M. Kukral, and 5054 typhimurium virulence John J. Mekalonas

IMMUNOLOGY

Double-negative (CD4-CD8-) lymphocytes bearing T-cell receptor a and ,B Veronika Groh, Marina Fabbi, Frans 5059 chains in normal human skin Hochstenbach, Richard T. Maziarz,

and Jack L. Strominger

Regulation of tumor necrosis factor gene expression in colorectal Stefan Beissert, Michael Bergholz, Inge 5064 adenocarcinoma: In vivo analysis by in situ hybridization Waase, Gerd Lepsien, Alfred Schauer,

Klaus Pfizenmaier, and Martin Kronke Recombinant interleukin 2 therapy in severe combined R. Pahwa, T. Chatila, S. Pahwa, C. 5069

immunodeficiency disease Paradise, N. K. Day, R. Geha, S. A. Schwartz, H. Slade, N. Oyaizu, and R. A. Good

Evidence for a functional idiotypic network among natural antibodies in Inger Lundkvist, Ant6nio Coutinho, 5074 normal mice Francisco Varela, and Dan Holmberg

A human immunoglobulin G receptor exists in both polypeptide-anchored and Bernard J. Scallon, Eileen Scigliano, 5079 phosphatidylinositol-glycan-anchored forms Victoria H. Freedman, May C. Miedel,

Yu-Ching E. Pan, Jay C. Unkeless, and Jarema P. Kochan

Embryonal carcinoma cells express Qa and Tla class I genes of the major Suzanne Ostrand-Rosenberg, Deborah A. 5084 histocompatibility complex Nickerson, Virginia K. Clements,

Elizabeth P. Garcia, Esi Lamouse-Smith, Leroy Hood, and Iwona Stroynowski

Positive selection of a T-cell subpopulation in the thymus in which it develops Kazuya Iwabuchi, Izumi Negishi, 5089 Hisashi Arase, Chikako Iwabuchi, Kazumasa Ogasawara, Robert A. Good, and Kazunori Onoe

Monoclonal antibodies specific to native murine T-cell receptor y8: Analysis of Shigeyoshi Itohara, Nobuki Nakanishi, 5094 -y8 T cells during thymic ontogeny and in peripheral lymphoid organs Osami Kanagawa, Ralph Kubo, and

Susumu Tonegawa

MEDICAL SCIENCES

Differential DNA sequence deletions from chromosomes 3, 11, 13, and 17 in A. Weston, J. C. Willey, R. Modali, H. 5099 squamous-cell carcinoma, large-cell carcinoma, and adenocarcinoma of the Sugimura, E. M. McDowell, J. Resau, human lung B. Light, A. Haugen, D. L. Mann, B.

F. Trump, and C. C. Harris Susceptibility to rheumatoid arthritis maps to a T-cell epitope shared by the Jean Roudier, Jorgen Petersen, Gary H. 5104

HLA-Dw4 DR ,3-1 chain and the Epstein-Barr virus glycoprotein gpllO Rhodes, Janos Luka, and Dennis A. Carson

Epididymis is a principal site of retrovirus expression in the mouse Ann Anderson Kiessling, Richard 5109 Crowell, and Cecil Fox

Inhibin and activin modulate the release of gonadotropin-releasing hormone, Felice Petraglia, Joan Vaughan, and 5114 human chorionic gonadotropin, and progesterone from cultured human Wylie Vale placental cells

Genomic DNA differences between pathogenic and nonpathogenic Egbert Tannich, Rolf D. Horstmann, 5118 Entamoeba histolytica Jurgen Knobloch, and Hans

Henning Arnold Detection of minimal disease in hematopoietic malignancies of the B-cell Masao Yamada, Sheila Hudson, Olivia 5123

lineage by using third-complementarity-determining region Tournay, Susan Bittenbender, Sara S. (CDR-III)-specific probes Shane, Beverly Lange, Yoshihide

Tsujimoto, Andrew J. Caton, and Giovanni Rovera

Vl

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Contents

Essential features of the P-glycoprotein pharmacophore as defined by a series H. L. Pearce, Ahmad R. Safa, N. J. 5128 of reserpine analogs that modulate multidrug resistance Bach, M. A. Winter, Margaret C.

Cirtain, and William T. Beck

Lead increases free Ca2+ concentration in cultured osteoblastic bone cells: Francis A. X. Schanne, Terry L. Dowd, 5133 Simultaneous detection of intracellular free Pb2+ by 19F NMR Raj K. Gupta, and John F. Rosen

Molecular dissection of a contiguous gene syndrome: Frequent submicroscopic David H. Ledbetter, Susan A. Ledbetter, 5136 deletions, evolutionarily conserved sequences, and a hypomethylated Peter vanTuinen, Kim M. Summers, "island" in the Miller-Dieker chromosome region Terence J. Robinson, Yusuke

Nakamura, Roger Wolff, Ray White, David F. Barker, Margaret R. Wallace, Francis S. Collins, and William B. Dobyns

Activation of protein kinase C by elevation of glucose concentration: Proposal Tian-Shing Lee, Kirstie A. Saltsman, 5141 for a mechanism in the development of diabetic vascular complications Hiromi Ohashi, and George L. King

Rate of internalization of an immunotoxin correlates with cytotoxic activity Ursula C. Wargalla and Ralph 5146 against human tumor cells A. Reisfeld

Selective displacement of nuclear proteins by antitumor drugs having affinity Jacek Bartkowiak, Jan Kapuscinski, 5151 for nucleic acids Myron R. Melamed, and Zbigniew

Darzynkiewicz

Extra-renal transcription of the renin genes in multiple tissues of mice and rats Marc Ekker, Diana Tronik, and 5155 Frangois Rougeon

Formation of nitric oxide from L-arginine in the central nervous system: A Richard G. Knowles, Miriam Palacios, 5159 transduction mechanism for stimulation of the soluble guanylate cyclase Richard M. J. Palmer, and

Salvador Moncada

MICROBIOLOGY

Functional role of human immunodeficiency virus type 1 vpu Ernest F. Terwilliger, Eric A. Cohen, 5163 Yichen Lu, Joseph G. Sodroski, and William A. Haseltine

Hepatitis B virus X gene activated KB-like enhancer sequences in the long Jr-Shin Twu, Keting Chu, and William S. 5168 terminal repeat of human immunodeficiency virus 1 Robinson

Penetration of human intestinal epithelial cells by Salmonella: Molecular Eric A. Elsinghorst, Louis S. Baron, and 5173 cloning and expression of Salmonella typhi invasion determinants in Dennis J. Kopecko Escherichia coli

NEUROBIOLOGY

Molecular cloning and primary structure of human glial fibrillary acidic protein Steven A. Reeves, Lee J. Helman, 5178 Audrey Allison, and Mark A. Israel

Visual responses in adult cat visual cortex depend on N-methyl-D- K. D. Miller, B. Chapman, and 5183 aspartate receptors M. P. Stryker

Deltorphins: A family of naturally occurring peptides with high affinity and Vittorio Erspamer, Pietro Melchiorri, 5188 selectivity for 8 opioid binding sites Giuliana Falconieri-Erspamer, Lucia

Negri, Rita Corsi, Cinzia Severini, Donatella Barra, Maurizio Simmaco, and Gunther Kreil

Substance P receptor biology sites are expressed by glia in vivo after Patrick W. Mantyh, Donald J. Johnson, 5193 neuronal injury Christian G. Boehmer, Mark D. Catton,

Harry V. Vinters, John E. Maggio, Heng-Phon Too, and Steven R. Vigna

Cholecystokinin evokes secretion of oxytocin and vasopressin from rat neural C. A. Bondy, R. T. Jensen, L. S. Brady, 5198 lobe independent of external calcium and H. Gainer

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: Unexpected Mark J. Alexander, Margaret A. Miller, 5202 abundance in hippocampus and subiculum Daniel M. Dorsa, Bryant P. Bullock,

Richard H. Melloni, Jr., Paul R. Dobner, and Susan E. Leeman

Voltage-gated calcium channels: Direct observation of the anomalous mole D. D. Friel and R. W. Tsien 5207 fraction effect at the single-channel level

PHYSIOLOGICAL SCIENCES

Reconstitution of an inwardly rectifying potassium channel from the basolateral James Costantin, Socorro Alcalen, 5212 membranes of Necturus enterocytes into planar lipid bilayers Angela de Souza Otero, William P.

Dubinsky, and Stanley G. Schultz

vii

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