( )

OR

AC

(m

ol-T

rolo

x /

mol

) ORAC

(Oleuropein, )

( )

0 5 10

tR(min)

DPPH: 1,1-Diphenyl-2-picrylhydrazyl

DPPH ( ) �max: 517 nm

DPPH

**: Area under the curve

AUC** ( )

AUC ( )

ORAC

Phenylahistin; PLH

�PLH

( ) TEL : 086-251-8297 E-mail : hkanzaki@cc.okayama-u.ac.jp

(Oleuropein, )

( )

0 5 10 tR(min)

OR

AC

(mol

-Tro

lox

/ mol

) ORAC

DPPH: 1,1-Diphenyl-2-picrylhydrazyl

DPPH ( )�max: 517 nm

DPPH

**: Area under the curve

*AUC***( )

AUC( )

ORAC

Oleuropein

Phenylahistin; PLH �PLH

(m

ol-T

rolo

x /

mol

)

DPPH

HPLC

0

2

4

6

8

10

1

High functionalization of olive leaf extract

High functionalization of olive leaf extract Hiroshi Kanzaki, Ayaka Takatsu, Teruhiko Nitoda Graduate School of Environmental and Life Science, Okayama University

Ant

ioxi

dant

act

ivity

(m

ol-tr

olox

eq.

/ m

ol)

Ant

ioxi

dant

act

ivity

O

RA

C v

alue

(m

ol-tr

olox

eq.

/ m

ol) DPPH test ORAC test

Microbial conversion of olive leaf extracts results in potent antioxidant compound, which is possibly utilized as cosmetic or food materials.

Olive leaves (containing oleuropein &

compound A)

Baker’s yeast Novel

compound (compound B)

Compound A Substrate

Compound B Product

0 5 10

Reaction with bakers’ yeasts

tR(min)

DPPH: 1,1-Diphenyl-2-picrylhydrazyl

DPPH radical(purple) lmax: 517 nm

Radical scavenging Decrease in absorbance

Antioxidant DPPH test Electron transfer

blank

Addition of Fluorescent

Probe

Addition of Radical initiator

Antioxidant **: Area under the curve

AUC** (blank)

AUC (sample)

ORAC test Hydrogen atom transfer

Enzyme from Streptmyces alblus

Compound B exhibited a pronounced antioxidant activity in both DPPH and ORAC tests.

Phenylahistin; PLH from Aspergillus ustus �PLH

Graduate School of Environmental and Life Science Hiroshi Kanzaki TEL : 086-251-8297 E-mail : hkanzaki@cc.okayama-u.ac.jp

ΔPLH prepared by microbial conversion is currently under development as an antineoplastic agent

HPLC chromatogram

Enzyme fromStreptmyces

yalblus

Phenylahistin; PLHfrom Aspergillus ustus

s�PLH

ΔPLH prepared by microbial conversion is currently under development as an antineoplastic agent

Example of high functionalization by bioconversion

Olive leaves (containing oleuropein &

compound A)

Novel compound

)(compound B)(compound B)

nd Aate

0 5 10

CompounSubstra

Compound BProduct

R ti ithReaction with Reaction withbakers’ yeasts

tR(min)

Baker’syeast

HPLC chromatogram

Microbial conversion of olive leaf constituent

Ant

ioxi

dant

act

ivity

(mol

-trol

oxeq

. / m

ol)

Ant

ioxi

dant

act

ivity

OR

AC

valu

e(m

ol-tr

olox

eq./

mol

)DPPH test ORAC test

Microbial conversion of olive leaf extracts results in potent antioxidant compound, which is possibly utilized as cosmetic or food materials.

DPPH: 1,1-Diphenyl-2-picrylhydrazyl

DPPH radical(purple)lmax: 517 nm

Radical scavengingDecrease in absorbance

AntioxidantDPPH test Electron transfer

blank

Addition of Fluorescent

Probe

Addition of Radical initiator

Antioxidant**: Area under the curve

*AUC***(blank)

AUC(sample)

ORAC test Hydrogen atom transfer

Compound B exhibited a pronounced antioxidant activity in both DPPH and ORAC tests.

Antioxidant activity of oleuropein analogs

0

2

4

6

8

10

1