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OUTOUT‐‐LINELINE
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Product Introduction
Application Background
Product Detail
Principle Overview
1. Product IntroductionIVD Equipment：Automated Blood Cell Morphology Analyzer (ME-150)
Blood Smears(INPUT)
ME-150 Digital Images & Report(OUTPUT)
Clinical Lab. Dept.
HematologyDept.
PathologyDept.
2. Application Background2.1 Blood Routine Examination
70%~80%Samples
20%~30% Samples ( ISLH 41 Rules, 2005 )
Report
Manual Morphology Analyzer
Screening Test
Diagnostic Test
2. Application Background2.2 Manual Differential Cell Analysis：dilemmaLabor‐intensive, time‐consumed
Supposed 1000 samples/day;
Abnormal for morphology check: 200-300 smears/day.Skilled technologist spends an average of 6 minutes/smear for morphology;Total time : 20-30 hours/day！！！
Lack of standards for subjectivity
Inconvenience for storing or sharing sample information
WE NEED AUTOMATED MORPHOLOGY ANALYZER!!
2. Application Background2.3 Development Review for Morphology Analyzer
1980s 1990s
2000s1970s
1960s
Semi-Automation
1. CELLSCAN/GLOPR,�Canada;2. LARC,�Corning�Electronics;3. Diff3,�Coulter;4. ADC-500,�Abbott;5. Microx,�Omron.
Microscopy Image
Judith,�Prewitt,�University�of�Pennsylvania
Color Image Capture
SAMBA-200,�Thomson-TITN Smaller Size, Better PerformanceCoulter�Gens,�Beckman�Coulter
Automation Digitizing
1. Easy�Cell�Assistant,�Medica;2. Vision�Hema,�WestMedica;3. HemaCAM,�Horn�Imaging;4. DM96 (2007) DM1200
(2009) DM9600(2014), Cellavision AB (Sweden);
5. ME-150 (2015), iCELL (China)
Microscopic Examination: Gold Standard
3. Product Detail3.1 System Components
Morphology Analyzer Workstation
MonitorMagazine & Smears
iCELL™ ME‐150
Printer
3. Product Detail3.2 Examination ItemsItems Cell Differential Counts Morphology Parameters
WBC
Band Neutrophil, Segmented Neutrophil, Lymphocyte, Monocyte, Eosinophil, Basophil, Blast cell, Promyelocyte, Myelocyte, Metamyelocyte, Atypical Lymphocyte.
Left shift, Right shift, Toxic granulation index, Lobulation index, Vacuolation, Mean area, Mean diameter, Area variant, Diameter variant.
Non‐WBCErythroblasts(NRBC), Giant thrombocytes,
Thrombocytes aggregations, Smudge cells and Artefacts
RBC
Erythrocytes, Microcytes, Macrocytes, Gigantocytes, Hypochromic erythrocytes, Hyperchromic erythrocytes, Teardrop cell, Elliptocytes, Target Cell, Unidentified.
Poikilocytosis, Rouleaux formation, Anisocytosis, Mean area, Mean diameter, Area variant, Diameter variant, Howell‐Jolly bodies, Pappenheimer bodies, Basophilic stippling, Parasites.
PLTSmall, normal, giant thrombocyte; Severe,
moderate and mild thrombocyte aggregations
Anisocytosis, Mean area, Mean diameter, Area variant, Diameter variant.
3. Product Detail3.3 Application Value
ISO15189:2012 CAP
Remote Diagnosis Joint Diagnosis Medical –Tech Education
3. Product Detail Test Velocity：30~35 smears/hour Image Resolution：0.13 µm/pixel（oil） Preclassification Accuracy：
3.4 Performance
MON%
LYM%
EOS%
BAS%
NEU%
MYE-meta%
MYE%
MYE-pro%
Blast%
NRBC%
ME-150 91.7 97.1 93.3 84.3 95.5 46.7 24.5 69.2 58 77.4
DM96(UCL) 87.6 94.8 79.9 54.1 99.5 32.6 37.7 77.6 76.6 80.6
DM96(CUF) 92 99 96 80 95.6 58 56
UCL: University College London CHU: University Caen France
3. Product Detail3.5 Highlight
ToxicGranulation
Lobulation
Nucleolus
Precise, Accurate Evidence-based Medical
NEU-b NEU-s MON
LYM BAS EOS
Abnormal Morphology Evaluation System
3. Product Detail3.6 Hardware
Hardware(Image)
Algorithms(Morphology)
Software(User Interface)
Report
3. Product Detail3.7 Software (Analyze)
VideoPatient Info.
Cell Info.
Operation Items
3. Product Detail3.7 Software (Review)
Menu
Cell Info.
Images
SampleList
Patient Info.
3. Product Detail3.7 Software (Report)
WBC
RBC & PLT
Morphology
3.Product Detail3.8 Specification
Product ME-150（iCELL) DM96 (Cellavision AB)
Items
LDC, WBC morphology WBC presorting
RBC morphology RBC qualitative analysis
PLT morphology PLT count
Toxic granulation Morphology Test, Quantitative Analysis None
Lens 10X/100X(Oil) 10*/50*(Oil)
Principle ANN、SVM ANN
Loading (once) 150 smears 96 smears
Max. Resolution 732×574 pixal 658×494 pixal
Emergency Function
Emergency Case Priority
Single Slide mode can be set
None
Data Memory Workstation (Parameter can be customized) 1G RAM, 160G ROM
4. Principle Overview4.1 Principle Outline
Monolayer PositioningMonolayer PositioningAuto-focusAuto-focus Cytological
ClassificationCytological
Classification
1.Quantitfication
Focus Function;
2. Focus Locating
3. Capture Images
1.Quantitfication
Focus Function;
2. Focus Locating
3. Capture Images
Core Algorithms
1.Quantitfication
Monolayer Function;
2. Scanning
3. Monolayer Locating
1.Quantitfication
Monolayer Function;
2. Scanning
3. Monolayer Locating
1.Image Segmentation
2. Feature Extraction
3. LDC Analysis
4. Morphology
1.Image Segmentation
2. Feature Extraction
3. LDC Analysis
4. Morphology
4. Principle Overview4.2 Leukocyte Differential Counts: Supported Vector Machine
SVM: Feature Space(Low Dimension)
Hilbert Space(High Dimension) Hyper plane
4. Principle Overview4.3 WBC Morphology Analysis
Left/Right Shift Specificity 98.9%, Sensitivity 76.7%
