1 Meridian Healthcare Professional Product List 2013 ImmunoDOT Test Professional Product List 2013 ImmunoDOT Test ImmunoDOT is an easy-to-use, visually interpreted, rapid-test format, dipstick ELISA that allows individual patients to be tested for multiple parameters simultaneously. Up to five or six different tests may be completed simultaneously on a single patient specimen, making it ideal for users who require fast, flexible and reliable diagnostic tests in a single patient format. ImmunoDOT is a sophisticated test format which is a multiparameter, nitrocellulose ELISA with sensitivities and specificities similar to reference method western blots. This format also equals or surpasses traditional ELISA formats for sensitivity and specificity. Meridian Healthcare medical diagnostics & equipment
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1 Meridian Healthcare Professional Product List 2013 ImmunoDOT Test
Professional Product List 2013
ImmunoDOT Test
ImmunoDOT is an easy-to-use, visually interpreted, rapid-test format, dipstick ELISA that allows individual patients to be tested for multiple parameters simultaneously. Up to five or six different tests may be completed simultaneously on a single patient specimen, making it ideal for users who require fast, flexible and reliable diagnostic tests in a single patient format. ImmunoDOT is a sophisticated test format which is a multiparameter, nitrocellulose ELISA with sensitivities and specificities similar to reference method western blots. This format also equals or surpasses traditional ELISA formats for sensitivity and specificity.
Meridian Healthcare medical diagnostics & equipment
2 Meridian Healthcare Professional Product List 2013 ImmunoDOT Test
Indice / Index
PRODUCT NAME PAGE
ImmunoDOT GenBio Line 3
ImmunoDOT GenBio Line Controls 3
EBV PANEL 4
HSV PANEL 5
AUTOIMMUNITY PANEL 1 6
AUTOIMMUNITY PANEL 6 7
THYROID AUTOIMMUNITY PANEL 8
BORRELIA PANEL 9
COXIELLA PANEL 10
INFO RICKETTSIA conorii 11
RICKETTSIA typhi PANEL 12
LEPTOSPIRA biflexa PANEL 13
SALMONELLA typhi PANEL 14
ImmunoDOT THERADIAG Line
CELIAC IgG PANEL 15
CELIAC IgA PANEL 15
ANCA/GBM PANEL 16
CUSTOMER SUPPORT 17
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PRODUCT INFO:
The ImmunoDOT AUTOIMMUNITY PANEL 1 is a qualitative test that detects:
Total ANA - ds-DNA - RNP/Sm - SS-A/SS-B
AUTOIMMUNITY PANEL 1 Method: 6-well membrane (Positive human IgG Control, Total Nuclear Antigen (ds-DNA), Ribonucleoprotein (RNP) and Smith antigens, Sjogren’s syndrome A and B antigens, Negative control), Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 45 min. at 44-48°C
Negative Control
Positive Control
TOTAL ANA
ds-DNA
RNP/Sm
SS-A/SS-B
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PRODUCT INFO:
The ImmunoDOT AUTOIMMUNITY PANEL 6 is a qualitative test that detects:
ScL-70 - Jo-1 - RNP/Sm - Sm - SS-A - SS-B
AUTOIMMUNITY PANEL 6 Method: 6-well membrane (Sjogren’s Syndrome antigen A (SS-A/Ro), Sjogren’s Syndrome antigen B (SS-B/La), ribonucleaoprotein (RNP), Smith (Sm) antigen, DNA topoisomerase I ( ScI-70) and histidyl tRNA synthetase (Jo-1), Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 1hr45 at 44-48°C
ScL-70
Jo-1
RNP/Sm
Sm
SS-A
SS-B
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PRODUCT INFO:
The ImmunoDOT THYROID AUTOIMMUNITY is a qualitative test that detects:
TPO and anti-TG
THYROID AUTOIMMUNITY Method: 6-well membrane (Positive human control, two levels of recombinant human thyroid peroxidase (TPO) protein (microsome), two levels of highly purified human thyroglobulin and negative control, Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 35 min. at 44-48°C
Negative Control
Positive Control
TPO Positivo basso
TPO Positivo
Anti-TG Positivo basso
Anti-TG Positivo
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PRODUCT INFO:
The ImmunoDOT BORRELIA Lyme is a qualitative test that detects:
BORRELIA Lyme IgA/IgG/IgM
BORRELIA Lyme Method: 6-well membrane (Positive human antibody control, negative control, B. burgdorferi (strain B31) partially purified sonicate of whole organism blended with purified P39 recombinant protein, partially purified sonicate of whole organism, P39 recombinant protein, and flagellin extracted and purified from B. burgdorferi (strain B31). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 35 min. at 44-48°C
Positive Control
Negative Control
P39 and anti-whole organism
anti-whole organism
P39
P41 Flagellina
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PRODUCT INFO:
The ImmunoDOT COXIELLA burnetii is a qualitative test that detects:
COXIELLA burnetii Method: 6-well membrane (Positive human antibody control, negative control). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 45 min. at 44-48°C
Positive Control
Negative Control
Phase II dilution 1
Phase II dilution 2
Phase II dilution 3
Phase I
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PRODUCT INFO:
The ImmunoDOT RICKETTSIA conorii is a qualitative test that detects:
Rickettsia conorii Mediterranean Spot Fever
Interpretazione:
Più alto è il numero di DOT positivi, maggiore è il livello di anticorpi presenti nel campione.
Nessun DOT positivo - reattivo, anticorpi non presenti nel campione.
Un risultato iniziale negativo seguito da successiva positività, indica siero conversione.
DOT 1 e 2 positivi – reattivi : il dosaggio deve essere ripetuto dopo 2/3 settimane.
DOT 1, 2, 3 e 4 positivi indicano la presenza di anticorpi specifici.
RICKETTSIA conorii Method: 6-well membrane (Positive human IgG control, negative control and four dilutions of Rickettsia conorii antigen). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 45 min at 44-48°C
Positive Control
Negative Control
Antigen dlution 1
Antigen dlution 2
Antigen dlution 3
Antigen dlution 4
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PRODUCT INFO:
The ImmunoDOT RICKETTSIA typhi is a qualitative test that detects:
IgG and IgM antibodies to Rickettsia typhi
Interpretazione:
Più alto è il numero di DOT positivi, maggiore è il livello di anticorpi presenti nel campione.
Nessun DOT positivo - reattivo, anticorpi non presenti nel campione.
Un risultato iniziale negativo seguito da successiva positività, indica siero conversione.
DOT 1 e 2 positivi – reattivi : il dosaggio deve essere ripetuto dopo 2/3 settimane.
DOT 1, 2, 3 e 4 positivi indicano la presenza di anticorpi specifici.
RICKETTSIA thyphi Method: 6-well membrane (Positive human IgG control, negative control and four dilutions of Rickettsia typhi antigen). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 45 min. at 44-48°C
Positive Control
Negative Control
Antigen dlution 1
Antigen dlution 2
Antigen dlution 3
Antigen dlution 4
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PRODUCT INFO:
The ImmunoDOT LEPTOSPIRA biflexa is a qualitative test that detects:
IgM antibodies to Leptospira biflexa - patoc 1
Interpretazione:
Nessun DOT positivo - reattivo, anticorpi non presenti nel campione.
DOT 1 e 2 positivi – reattivi : reazione borderline - ripetere il saggio.
DOT 1, 2, 3 e 4 positivi indicano la presenza di anticorpi specifici.
RICKETTSIA thyphi Method: 6-well membrane (Positive human IgM control, negative control and four dilutions of Leptospira biflrxa antigen). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 40 min. at 44-48°C
Positive Control
Negative Control
Dilution 1
Dilution 2
Dilution 3
Dilution 4
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PRODUCT INFO:
The ImmunoDOT SALMONELLA typhi is a qualitative test that detects:
antibodies to Salmonella - typhoid fever
SALMONELLA thyphi Method: 6-well membrane (Positive human control, negative control and Salmonella typhi antigens, “O”, “H” and Vi). Conjugate: anti-human ANTIBODIES ALP, Substrate: BCIP/NBT, incubation: 40 min. at 44-48°C
IgG/IgM Positive Control ( Negative Control is area around dots
Salmonella H, type a
Salmonella H, type b
Salmonella H, type d
Salmonella O, type Vi
Salmonella O, type D
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PRODUCT INFO:
The ImmunoDOT CELIAC IgG and IgA is a qualitative EIA based on membrane assay strip for autoantibodies detection.
CELIAC IgA: simultaneus detection of human Gliadin and Tissue Transglutaminase.
CELIAC IgG: simultaneus detection of human Gliadin and Tissue Transglutaminase.
CELIAC IgA / IgG Method: 3-well membrane (Positive human control, human Gliadin and Tissue Transglutaminase). Conjugate: Phosphatase Alkalin labeled IgA and IgG, Substrate: BCIP/NBT, incubation: 90 min. at R.T.
Positive Control
Gliadin
tTG
CELIAC IgA
CELIAC IgG
tTG
Gliadin
Positive Control
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PRODUCT INFO:
The ImmunoDOT ANCA-GBM is a qualitative EIA for the simultaneous detection of:
Anti-neutrophil cytoplasm antibodies ANCA directed against Myeloperoxidase MPO and Serine Proteinase 3 PR3.
Anti-glomerular basement membrane GBM antibodies using a purified antigen based on type IV.
ANCA-GBM Method: 4-well membrane (Positive control, human purified Myeloperoxydase, human purified proteinase 3, Bovine purified Goodpasture antigen). Conjugate: Phosphatase Alkalin labeled protein A , Substrate: BCIP/NBT, incubation: 107 min. at R.T.
