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Lec #5 Discuss the Nitrogen Cycle.pptx

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    Discuss the nitrogen cycle

    Plant Physioloty

    1/28/09Lec#4

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    Distinguish between symbiotic and

    asymbiotic with respect to nitrogen

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    Plant forms a relationship with rhizobium

    (nodules) in roots in symbiotic relationship.

    Rhzobium fixes Nitrogen which becomes

    available to plant

    Asymbiotic

    Azospirilum is in the rhizosphere (area of soil in

    close association with the roots)

    Found in sweet potato nitrogen is in the organic

    form

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    List two major symptom of

    nitrogen deficiency in plants?

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    Stunted growth

    Yellowing of the older leaves (choroses)

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    List effect of phosphorus in

    plants

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    Increase growth like N (but to lesser extent)

    Part of genetic material

    RNA, DNA, purine, pyrimadine

    Part of energy transformation (ATP)_ Spurs early and rapid growth (starter fertilizer)

    Improves water intake

    Helps resist cold and disease

    Improves quality of produce (grains and fruits)

    Improves efficiency of N uptake

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    What are the two general rule of

    thumb for P as it relate to plant

    growth?

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    P more important for plants with floral parts

    Broccoli

    Cauliflour

    P is taken up as a phospate ion

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    How much P is in an acre of plow

    layer and how much is available to

    plants?

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    800 1600 lbs

    Only 4 lbs is available

    The rest is tide up based on the pH

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    At what pH is Phosphorus

    available to plants?

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    Between pH 6.5 and 7.0

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    List the different species of P, and at

    what pH they insoluble and

    therefore not available to plants?

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    Species of P

    FePO4

    AlPO4

    CaPO4

    Insoluble at pH

    3.5 - 4.5

    4.0 - 6.5

    7 - 9

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    List four important factors

    associated with the mineralization of

    phosphorus.

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    1. Involves Microorganisms from organic matter

    2. More rapid in virgin soil

    3. Process favors high temp

    1. Thermophilic (45-65 C)

    2. Mesophilic (25 35 C) the optimum

    4. Phosphorus uptake correlated withmineralization rate

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    What is the impact of organisms

    on P mineralization?

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    Organisms produce enzymes

    Phosphotases (help cleave phosphorus)

    Organisms are pH dependent

    Acid

    Alkaline

    Phosphotases / specific for organic

    phosphotase key to attack

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    List two important enzymes in soil

    and how they are broken down.

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    Inositol phosphate

    Found when phytases are present

    Broken down by organism

    Phytases

    Liberates P from phytin (or phytic acid)

    Is broken down to penta, tetra, tri, di, and

    monophosphates

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    What is vernalization?

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    Refers to the amount of chilling hours needed for budding to occur. Aftervernilization the plant is returned to normal temperature.

    Many temperateplants have a vernalization requirement and must experience aperiod of low winter temperature to initiate or accelerate the flowering process,or, as the case with many fruit treespecies, to actually break dormancy, prior to

    flowering. Many plant species, includingArabidopsis thaliana and winter cerealssuch as wheat, must go through a prolonged period of cold before floweringoccurs. This ensures that reproductive development and seed production occurs atthe optimum environmentally favorable time, normally following the passing ofwinter. The needed cold is often expressed in chill hours.

    Following vernalization, plants have acquired the competence to flower, althoughthey may require additional seasonal cues or weeks of growth before they will

    actually flower. One of the most important influences that temperature has on thefloral transition is the vernalization response. Some plant species do not flowerwithout vernalization. Many biennial species have a vernalization period, whichcan vary in period and temperature. Typical vernalization temperatures arebetween 5 and 10 degrees Celsius (40 and 50 degrees Fahrenheit).

    http://en.wikipedia.org/wiki/Temperatehttp://en.wikipedia.org/wiki/Plantshttp://en.wikipedia.org/wiki/Flowerhttp://en.wikipedia.org/wiki/Fruit_treehttp://en.wikipedia.org/wiki/Specieshttp://en.wikipedia.org/wiki/Flowerhttp://en.wikipedia.org/wiki/Fruit_treehttp://en.wikipedia.org/wiki/Specieshttp://en.wikipedia.org/wiki/Arabidopsis_thalianahttp://en.wikipedia.org/wiki/Arabidopsis_thalianahttp://en.wikipedia.org/wiki/Cerealshttp://en.wikipedia.org/wiki/Arabidopsis_thalianahttp://en.wikipedia.org/wiki/Cerealshttp://en.wikipedia.org/wiki/Wheathttp://en.wikipedia.org/wiki/Chilling_requirementhttp://en.wikipedia.org/wiki/Chilling_requirementhttp://en.wikipedia.org/wiki/Biennial_planthttp://en.wikipedia.org/wiki/Biennial_planthttp://en.wikipedia.org/wiki/Biennial_planthttp://en.wikipedia.org/wiki/Chilling_requirementhttp://en.wikipedia.org/wiki/Wheathttp://en.wikipedia.org/wiki/Cerealshttp://en.wikipedia.org/wiki/Arabidopsis_thalianahttp://en.wikipedia.org/wiki/Specieshttp://en.wikipedia.org/wiki/Fruit_treehttp://en.wikipedia.org/wiki/Flowerhttp://en.wikipedia.org/wiki/Plantshttp://en.wikipedia.org/wiki/Temperate
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    Distinguish between

    translocation and mobilization

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    Translocation refers to the uptake of nutrients

    by plant and moving them to tissues.

    Mobilization refers to the movement of

    water and nutrients in higher plants.

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    Name two conducting tissues in

    plant and their function.

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    Xylem- involved in theuptake of water & somenutrients (via mass flow). It is the woody part of the

    plant

    Covered by a thin layer(cambium- dividing)

    Phloem transportsphotosynthates for

    carbohydrates from leavesto lower part of plants(roots) The outer part of the plant is

    dead phloem (bark)

    http://upload.wikimedia.org/wikipedia/commons/e/ed/Stem-cross-section2.jpg
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    Consequences of Phosphorus

    deficiency in plants

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    Stunting but plant

    remain green

    Purplish tint on leaves

    (lower and older leaves) Delayed crop maturity

    Poor root system

    (especially in carrots)

    Purplish tint

    on leaves

    Poor root system

    http://images.google.com/imgres?imgurl=http://www.steve.gb.com/images/science/phosphorus_deficiency.png&imgrefurl=http://www.steve.gb.com/science/plant_mineral

    _nutrition.html&usg=__yhJBnXzeX4g8VbCgi6COn2m6Aek=&h=383&w=244&sz=12&hl=en&start=2&um=1&tbnid=vMddcOipE-wb8M:&tbnh=123&tbnw=78&prev=/images%3Fq%3Dplants%2Bwith%2Bphosphorus%2Bdeficeincy%26um%3D1%26hl%3Den%26safe%3Dactive%26rlz%3D1T4RNWE

    _enUS315US315%26sa%3DN&safe=on

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    List 5 reason potassium is

    important to plants.

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    K is not part of any organic plant cmpd

    Role in enzyme activator in protein

    Starch cellulose and lignin formation

    Regulates stomata opening (play role in gas exchange)

    Instrumental in moving (Pn) sugars Impo in fruit and vege (and root crop)

    Important in development and ripening of fruits and root(tuber) crops

    After six weeks of planting, give a shot of K, helps in root production Lends integrity to plant structure (due to role in activating

    enzymes involved in lignin.

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    Describe the mobility of K and P

    in soils

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    K moves readily in soils

    P does not move readily in soils, they are

    immobile.

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    What is marginal chlorosis?

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    Similar to chlorine toxicity.

    Due to salt damage and low moisture

    This is why after snow fall plant near

    highway/roads are

    Symptoms of severe K deficiency in a soybean

    leaf (left) compared to a leaf from a K-sufficient

    plant (right).

    Soybean plants exhibiting K-deficiency symptoms

    only on lower leaves. This indicates that K is

    mobile in soybean and is preferentially transported

    to young tissues when deficiencies exist.

    http://www.agry.purdue.edu/images/kteam/big/kteam7.jpg
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    What is the role of Ca++ in plants

    (list at least 3)?

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    Mainly build cell walls

    Iie, Calcium pectate= cement cell walls together

    and responsible for crunchiness in apples.

    Actively dividing cells need more

    Play role in protein and carbohydrate

    formation

    Role in seed formation

    Relatively immobile in plants

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    List 3 consequences of Ca++

    deficiency.

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    In new growth causes

    tip dieback

    In fruits blossom-end

    rot (BER) Water cone in apples

    ( collapsing of fruit cell

    walls)

    Empty pods (in

    peanuts)

    Browning and die back

    from the tip of a plant.

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    List the steps in translocation

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    1. Centripetal

    1. Water movement from outside in

    2. Toward xylem vessels of the central cylinder

    2. Vertical transport

    1. Roots to leaves

    3. Release of water as gaseous molecules at

    the plant/atmosphere interfaces

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    In a diagram show how water

    transport occurs in the roots.

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    leaf

    petiole

    stem

    xylem

    transpiration

    Caspirian strip

    cortexendodermis

    epidermis

    water

    Free

    space

    2 Water transport pathways in the higher plant of a young root from

    epidermis to the xylem. In young roots plants uses both apoplastic and

    symplastic transport. In older plants, the plasmodesmata is developed and

    the casparian strip is more resistant. Water transport is via the symplastic

    pathway only.

    Soil

    Present high

    resistance to water and

    solute transport. As

    plant gets older, it gets

    more resistant

    Separates cortex from central cylinderGenerally characterized by a suberized

    layer

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    Distinguish between symplastic and

    apoplastic transport pathways.

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    Apoplastic Seen in young root only

    Characterixed withplasmodesmata

    Transport is through free space

    in root tissue

    Symplastic

    Most important

    Transports nutrients also (Ca++)

    Also Seen in young roots Water transport is through the

    cytoplasm of root cells

    Only on older roots

    http://en.wikipedia.org/wiki/File:Apoplast_and_symplast_pathways.GIF

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