南医研究前沿投稿模板

细胞外囊泡/外泌体研究成果速览 （一）南方医科大学南方医院检验医学科 郑磊教授团队

序号 标题 来源期刊 IF2018 入藏号 DOI

1

Minimal Information for Studies of Extracellular Vesicles 2018

(MISEV2018): A Position Statement of The International

Society for Extracellular Vesicles and Update of The MISEV2014

Guidelines

J Extracell Vesicles 11.0 WOS:00045206890000110.1080/20013078.2018.15

35750

2

Defining Mesenchymal Stromal Cell (MSC)-Derived Small Extracellular Vesicles for Therapeutic Applications

J Extracell Vesicles 11.0 WOS:00046610480000110.1080/20013078.2019.16

09206

3

Exosomes Serve as Tumour Markers for Personalized

Diagnostics Owing to Their Important Role in Cancer

Metastasis

J Extracell Vesicles 11.0 暂无 10.3402/jev.v4.27522

4Characteristics and Roles of Exosomes in Cardiovascular

DiseaseDNA Cell Biol 2.918 WOS:000395562600003 10.1089/dna.2016.3496

5

Extracellular Vesicles in Type 2 Diabetes Mellitus: Key Roles in

Pathogenesis, Complications, and Therapy

J Extracell Vesicles 11.0 暂无 10.1080/20013078.2019.1625677

6

The Roles of Extracellular Vesicles in Gastric Cancer

Development, Microenvironment, Anti-Cancer Drug Resistance, and

Therapy

Mol Cancer 10.679 WOS:00046294190000810.1186/s12943-019-0967-

5

7Characteristics of Erythrocyte-Derived Microvesicles and Its Relation with Atherosclerosis

Atherosclerosis 4.255 WOS:00038950970004910.1016/

j.atherosclerosis.2016.10.043

南医研究前沿投稿模板1. Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018): A Position Statement of The International Society for Extracellular Vesicles and Update of The MISEV2014 Guidelines

期刊年卷：J Extracell Vesicles 2018;7(1)

DOI: 10.1080/20013078.2018.1535750 IF2018 = 11.0

作者列表Théry Clotilde, Witwer Kenneth W, Aikawa Elena, Alcaraz Maria Jose, Anderson Johnathon D, Andriantsitohaina Ramaroson, Antoniou

Anna, Arab Tanina, Archer Fabienne, Atkin-Smith Georgia K, Ayre D Craig, Bach Jean-Marie, Bachurski Daniel, Baharvand Hossein,

Balaj Leonora, Baldacchino Shawn, Bauer Natalie N, Baxter Amy A, Bebawy Mary, Beckham Carla, Bedina Zavec Apolonija,

Benmoussa Abderrahim, Berardi Anna C, Bergese Paolo, Bielska Ewa, Blenkiron Cherie, Bobis-Wozowicz Sylwia, Boilard Eric, Boireau

Wilfrid, Bongiovanni Antonella, Borràs Francesc E, Bosch Steffi, Boulanger Chantal M, Breakefield Xandra, Breglio Andrew M,

Brennan Meadhbh Á, Brigstock David R, Brisson Alain, Broekman Marike Ld, Bromberg Jacqueline F, Bryl-Górecka Paulina, Buch

Shilpa, Buck Amy H, Burger Dylan, Busatto Sara, Buschmann Dominik, Bussolati Benedetta, Buzás Edit I, Byrd James Bryan, Camussi

Giovanni, Carter David Rf, Caruso Sarah, Chamley Lawrence W, Chang Yu-Ting, Chen Chihchen, Chen Shuai, Cheng Lesley, Chin

Andrew R, Clayton Aled, Clerici Stefano P, Cocks Alex, Cocucci Emanuele, Coffey Robert J, Cordeiro-da-Silva Anabela, Couch Yvonne,

Coumans Frank Aw, Coyle Beth, Crescitelli Rossella, Criado Miria Ferreira, D'Souza-Schorey Crislyn, Das Saumya, Datta Chaudhuri

Amrita, de Candia Paola, De Santana Eliezer F, De Wever Olivier, Del Portillo Hernando A, Demaret Tanguy, Deville Sarah, Devitt

Andrew, Dhondt Bert, Di Vizio Dolores, Dieterich Lothar C, Dolo Vincenza, Dominguez Rubio Ana Paula, Dominici Massimo, Dourado

Mauricio R, Driedonks Tom Ap, Duarte Filipe V, Duncan Heather M, Eichenberger Ramon M, Ekström Karin, El Andaloussi Samir, Elie-

Caille Celine, Erdbrügger Uta, Falcón-Pérez Juan M, Fatima Farah, Fish Jason E, Flores-Bellver Miguel, Försönits András, Frelet-

Barrand Annie, Fricke Fabia, Fuhrmann Gregor, Gabrielsson Susanne, Gámez-Valero Ana, Gardiner Chris, Gärtner Kathrin, Gaudin

Raphael, Gho Yong Song, Giebel Bernd, Gilbert Caroline, Gimona Mario, Giusti Ilaria, Goberdhan Deborah Ci, Görgens André, Gorski

Sharon M, Greening David W, Gross Julia Christina, Gualerzi Alice, Gupta Gopal N, Gustafson Dakota, Handberg Aase, Haraszti Reka A,

Harrison Paul, Hegyesi Hargita, Hendrix An, Hill Andrew F, Hochberg Fred H, Hoffmann Karl F, Holder Beth, Holthofer Harry,

Hosseinkhani Baharak, Hu Guoku, Huang Yiyao, Huber Veronica, Hunt Stuart, Ibrahim Ahmed Gamal-Eldin, Ikezu Tsuneya, Inal Jameel

M, Isin Mustafa, Ivanova Alena, Jackson Hannah K, Jacobsen Soren, Jay Steven M, Jayachandran Muthuvel, Jenster Guido, Jiang

Lanzhou, Johnson Suzanne M, Jones Jennifer C, Jong Ambrose, Jovanovic-Talisman Tijana, Jung Stephanie, Kalluri Raghu, Kano Shin-

Ichi, Kaur Sukhbir, Kawamura Yumi, Keller Evan T, Khamari Delaram, Khomyakova Elena, Khvorova Anastasia, Kierulf Peter, Kim

Kwang Pyo, Kislinger Thomas, Klingeborn Mikael, Klinke David J, Kornek Miroslaw, Kosanović Maja M, Kovács Árpád Ferenc,

Krämer-Albers Eva-Maria, Krasemann Susanne, Krause Mirja, Kurochkin Igor V, Kusuma Gina D, Kuypers Sören, Laitinen Saara,

Langevin Scott M, Languino Lucia R, Lannigan Joanne, Lässer Cecilia, Laurent Louise C, Lavieu Gregory, Lázaro-Ibáñez Elisa, Le Lay

Soazig, Lee Myung-Shin, Lee Yi Xin Fiona, Lemos Debora S, Lenassi Metka, Leszczynska Aleksandra, Li Isaac Ts, Liao Ke, Libregts

Sten F, Ligeti Erzsebet, Lim Rebecca, Lim Sai Kiang, Linē Aija, Linnemannstöns Karen, Llorente Alicia, Lombard Catherine A,

Lorenowicz Magdalena J, Lörincz Ákos M, Lötvall Jan, Lovett Jason, Lowry Michelle C, Loyer Xavier, Lu Quan, Lukomska Barbara,

Lunavat Taral R, Maas Sybren Ln, Malhi Harmeet, Marcilla Antonio, Mariani Jacopo, Mariscal Javier, Martens-Uzunova Elena S,

Martin-Jaular Lorena, Martinez M Carmen, Martins Vilma Regina, Mathieu Mathilde, Mathivanan Suresh, Maugeri Marco, McGinnis

Lynda K, McVey Mark J, Meckes David G, Meehan Katie L, Mertens Inge, Minciacchi Valentina R, Möller Andreas, Møller Jørgensen

Malene, Morales-Kastresana Aizea, Morhayim Jess, Mullier François, Muraca Maurizio, Musante Luca, Mussack Veronika, Muth Dillon

C, Myburgh Kathryn H, Najrana Tanbir, Nawaz Muhammad, Nazarenko Irina, Nejsum Peter, Neri Christian, Neri Tommaso, Nieuwland

南医研究前沿投稿模板Rienk, Nimrichter Leonardo, Nolan John P, Nolte-'t Hoen Esther Nm, Noren Hooten Nicole, O'Driscoll Lorraine, O'Grady Tina,

O'Loghlen Ana, Ochiya Takahiro, Olivier Martin, Ortiz Alberto, Ortiz Luis A, Osteikoetxea Xabier, Østergaard Ole, Ostrowski Matias,

Park Jaesung, Pegtel D Michiel, Peinado Hector, Perut Francesca, Pfaffl Michael W, Phinney Donald G, Pieters Bartijn Ch, Pink Ryan C,

Pisetsky David S, Pogge von Strandmann Elke, Polakovicova Iva, Poon Ivan Kh, Powell Bonita H, Prada Ilaria, Pulliam Lynn,

Quesenberry Peter, Radeghieri Annalisa, Raffai Robert L, Raimondo Stefania, Rak Janusz, Ramirez Marcel I, Raposo Graça, Rayyan

Morsi S, Regev-Rudzki Neta, Ricklefs Franz L, Robbins Paul D, Roberts David D, Rodrigues Silvia C, Rohde Eva, Rome Sophie,

Rouschop Kasper Ma, Rughetti Aurelia, Russell Ashley E, Saá Paula, Sahoo Susmita, Salas-Huenuleo Edison, Sánchez Catherine,

Saugstad Julie A, Saul Meike J, Schiffelers Raymond M, Schneider Raphael, Schøyen Tine Hiorth, Scott Aaron, Shahaj Eriomina, Sharma

Shivani, Shatnyeva Olga, Shekari Faezeh, Shelke Ganesh Vilas, Shetty Ashok K, Shiba Kiyotaka, Siljander Pia R-M, Silva Andreia M,

Skowronek Agata, Snyder Orman L, Soares Rodrigo Pedro, Sódar Barbara W, Soekmadji Carolina, Sotillo Javier, Stahl Philip D,

Stoorvogel Willem, Stott Shannon L, Strasser Erwin F, Swift Simon, Tahara Hidetoshi, Tewari Muneesh, Timms Kate, Tiwari Swasti,

Tixeira Rochelle, Tkach Mercedes, Toh Wei Seong, Tomasini Richard, Torrecilhas Ana Claudia, Tosar Juan Pablo, Toxavidis Vasilis,

Urbanelli Lorena, Vader Pieter, van Balkom Bas Wm, van der Grein Susanne G, Van Deun Jan, van Herwijnen Martijn Jc, Van Keuren-

Jensen Kendall, van Niel Guillaume, van Royen Martin E, van Wijnen Andre J, Vasconcelos M Helena, Vechetti Ivan J, Veit Tiago D,

Vella Laura J, Velot Émilie, Verweij Frederik J, Vestad Beate, Viñas Jose L, Visnovitz Tamás, Vukman Krisztina V, Wahlgren Jessica,

Watson Dionysios C, Wauben Marca Hm, Weaver Alissa, Webber Jason P, Weber Viktoria, Wehman Ann M, Weiss Daniel J, Welsh

Joshua A, Wendt Sebastian, Wheelock Asa M, Wiener Zoltán, Witte Leonie, Wolfram Joy, Xagorari Angeliki, Xander Patricia, Xu Jing,

Yan Xiaomei, Yáñez-Mó María, Yin Hang, Yuana Yuana, Zappulli Valentina, Zarubova Jana, Žėkas Vytautas, Zhang Jian-Ye, Zhao

Zezhou, Zheng Lei, Zheutlin Alexander R, Zickler Antje M, Zimmermann Pascale, Zivkovic Angela M, Zocco Davide, Zuba-Surma Ewa

K

文章简介在过去十年中，关于细胞外囊泡的生理和病理功能性研究急剧猛增，但是

目前基于细胞外囊泡异质性较大这一生理特性，针对细胞外囊泡的分离提纯以

及标准化定量、表征方法仍存在较大的争议和阻碍。该文由国际细胞外囊泡学

会 （ ISEV ） 成 员 共 同 撰 写 ，发表于细胞外囊泡研究领域著名学术期刊

《Journal of Extracellular Vesicles》上，提出了细胞外囊泡研究的基本要

求和共识标准，内容涵盖了外泌体的收集、分离、提纯、表征以及功能学研究

方法， 是开展细胞外囊泡（含外泌体）研究的国际指南，也是 EV 研究成果投

南医研究前沿投稿模板稿 JEV 必须遵循的基本要求。

2. Defining Mesenchymal Stromal Cell (MSC)-Derived Small Extracellular Vesicles for Therapeutic Applications

期刊年卷：J Extracell Vesicles 2019;8(1)

DOI: 10.1080/20013078.2019.1609206 IF2018 = 11.0

作者列表Witwer Kenneth W, Van Balkom Bas W M, Bruno Stefania, Choo Andre, Dominici Massimo, Gimona Mario, Hill Andrew F, De Kleijn Dominique, Koh Mickey, Lai Ruenn Chai, Mitsialis S Alex, Ortiz Luis A, Rohde Eva, Asada Takashi, Toh Wei Seong, Weiss Daniel J, Zheng Lei, Giebel Bernd, Lim Sai Kiang

文章简介 近年来,研究发现干细胞来源的外泌体在组织器官再生修复过程中具有巨

大的应用潜力，与组织器官移植相比，其免疫原性大大降低，可作为纳米级的

治疗手段，因此受到了广泛的关注。但由于外泌体具有较大的生物异质性，同

时缺乏标准化的分离和提纯方法、质量检测手段，因而干细胞来源外泌体的临

南医研究前沿投稿模板床应用价值仍受到较大的争议。为此，新加坡细胞外囊泡临床研究与转化学会

（ SOCRATES) 、国际细胞外囊泡学会（ ISEV) 、国际细胞与基因治疗学会

（ ISCT ）、国际输血学会（ ISBT) 等四个学会的专家联合就此问题达成了共识 ，

提出了对于干细胞来源的外泌体，应建立可定量化评价其生物学特性的方法及

参 考 物 质 。该文发表于国际著名学术期刊《 Journal of Extracellular

Vesicles》上，为干细胞来源外泌体的治疗研究与应用提供了指导性建议。

3. Exosomes Serve as Tumour Markers for Personalized Diagnostics Owing to Their Important Role in Cancer Metastasis

期刊年卷：J Extracell Vesicles 2015;4:27522

DOI: 10.3402/jev.v4.27522 IF2018 = 11.0

作者列表An Taixue, Qin Sihua, Xu Yong, Tang Yueting, Huang Yiyao, Situ Bo, Inal Jameel M, Zheng Lei

文章简介外泌体是指在生理和病理状态下，机体细胞形成多泡小体后，通过细胞膜

融合分泌到细胞外环境中的直径为 30-150 nm左右的微小囊泡。系列研究表

明，外泌体在肿瘤疾病的发生发展、肿瘤转移等病理过程中发挥着重要的作用，

南医研究前沿投稿模板近年来，肿瘤细胞来源外泌体的生物学功能和潜在临床价值受到了广泛关注。

该综述发表于国际著名学术期刊《 Journal of Extracellular Vesicles》上，

系统介绍了外泌体在肿瘤的发生、发展以及辅助肿瘤细胞穿越内皮血管进入血

液循环过程中的作用，讨论了外泌体作为疾病诊断标志物的应用前景及面临的

挑战， 是外泌体相关肿瘤研究领域的经典综述之一。

南医研究前沿投稿模板

4. Characteristics and Roles of Exosomes in Cardiovascular Disease

期刊年卷：DNA Cell Biol. 2017 Mar;36(3)

DOI: 10.1089/dna.2016.3496 IF2018= 2.918IF2017= 2.634

作者列表Zhang Yuan, Hu Yan-Wei, Zheng Lei

文章简介心血管疾病（CVD）是全球死亡的主要原因之一，且发病率和死亡率仍在

不断攀升，给社会和医疗保健系统带来了巨大的经济负担。虽然现今临床上对

于心脏疾病的诊断及治疗已经发生了巨大的进展，如优化的冠状动脉支架，但

是缺血再灌注损伤的结果仍然不能令人满意，且当 CVD发展为肥大和心力衰竭

时则不能逆转。在这种情况下，细胞外囊泡的出现为早期发现心血管疾病风险

人群的病理变化提供了新的思路。本综述总结了外泌体在 CVD 相关的各种生

理和病理环境中的性质和作用，对 CVD 领域的外泌体研究提出了关键的几个

建设性问题，对于 CVD 领域的细胞外囊泡研究极具参考价值。

南医研究前沿投稿模板

Fig. The role of exosomes in atherosclerosis. An outline of the known cellular communication pathways that exosomesare known to

participate in with regard to the development of atherosclerosis. For a detailed explanation of the pathways, see text on exosomes in

atherosclerosis section. DCs, dendritic cells; ECs, endothelial cells; ox-LDL, oxidized LDL; miR, microRNA; PS, phosphatidylserine;

VSMCs, vascular smooth muscle cells.

5. Extracellular Vesicles in Type 2 Diabetes Mellitus: Key Roles in Pathogenesis, Complications, and Therapy

期刊年卷：J Extracell Vesicles 2019 Jun;8(1)

DOI: 10.1080/20013078.2019.1625677IF2018 =11.0

作者列表Xiao Yongwei, Zheng Lei, Zou Xiaofeng, Wang Jigang, Zhong Jianing, Zhong Tianyu

文章简介据世界卫生组织报告，全球糖尿病患者已达 4.2亿，其中绝大多数为 2型

糖尿病。中国是世界上糖尿病患者最多的国家，人数超过 1亿，发病人数还在

南医研究前沿投稿模板大幅攀升。细胞外囊泡是细胞分泌的 30到 2000nm大小不等的囊泡状小体，

可参与细胞间的信号交流和代谢调控。近年来，细胞外囊泡在糖尿病中的作用

引起了广泛的关注。该文章发表于国际著名学术期刊《 Journal of

Extracellular Vesicles》上，系统介绍了细胞外囊泡在 2 型糖尿病的发生发

展、并发症和治疗中的重要作用，为细胞外囊泡在糖尿病诊治中的应用提供了

诸多可行的新思路。

6. The Roles of Extracellular Vesicles in Gastric Cancer Development, Microenvironment, Anti-Cancer Drug Resistance, and Therapy

期刊年卷：Mol. Cancer 2019 03 30;18(1)

DOI: 10.1186/s12943-019-0967-5 IF2018= 10.679

作者列表Huang Tingting, Song Chunli, Zheng Lei, Xia Ligang, Li Yang, Zhou Yiwen.

文章简介胃癌，基于其诊断延迟和转移频率高等特点，是癌症相关死亡的主要原因

之一。由于生物活性分子可被递送到受体细胞以影响它们的生物学特性，并改

变其周围的微环境，而这些生物分子几乎都包含于细胞外囊泡（EV）中。因此，

南医研究前沿投稿模板对细胞外囊泡的研究有助于对胃癌生长、转移、免疫逃避以及化疗耐药等机制

的深入认识。该综述总结了胃癌衍生的 EV 对肿瘤微环境的影响以及微环境衍

生的 EV 在胃癌中的功能，并讨论了肿瘤与微环境之间的双向通信对胃癌生长 、

转移行为、免疫应答以及耐药性的影响，可为 EV 在胃癌中的作用研究提供有

价值的参考。

Fig. The functional network of cancer derived EVs in GC microenvironment. GC cells-derived EVs promote angiogenesis via releasing

miR-130a. Pericytes, MSCs, and fibroblasts absorbed EVs to induce CAFs transformation in tumor microenvironment through different

pathway or miRNAs in cells. The functions of cancer cells-derived EVs in adipocytes differentiation. Different immune cells in tumor

microenvironment can be affected by tumor-derived EVs. GC-derived EVs inhibit T cell immunity, polarize neutrophils to a pro-tumor

phenotype, induce macrophages to release more proinflammatory factors and active Th17 to promote cancer progression. Abbreviations:

GC, gastric cancer; MSC, mesenchymal stem cell; CAF, cancer-associated fibroblast.

7. Characteristics of Erythrocyte-Derived Microvesicles and Its Relation with Atherosclerosis

期刊年卷：Atherosclerosis 2016 12;255

DOI: 10.1016/j.atherosclerosis.2016.10.043 IF2018= 4.255IF2016=4.239

南医研究前沿投稿模板作者列表Li Kai-Yin, Zheng Lei, Wang Qian, Hu Yan-Wei.

文章简介动脉粥样硬化是一种常见的血管疾病。红细胞作为最丰富的血细胞类型，

在动脉粥样硬化的发生发展中起着非常重要的作用，已有文献报道，红细胞来

源的微泡（ErMVs）可通过促凝、介导炎症以及诱导细胞粘附来促进动脉粥样

硬化形成。该综述总结了 ErMVs 的形成、降解相关机制，并讨论了 ErMVs

在动脉粥样硬化中的可能作用机制以及其在心血管疾病中的潜在临床应用价值 ，

为动脉粥样硬化疾病的诊断和预后提供了新的研究思路。

Fig. Erythrocyte-derived microvesicles formation and degradation. When erythrocytes encounter specific stimuli, such as shear stress,

complement attack, agonist (or proapoptotic) stimulation, or damage, they are prone to form microvesicles (MVs) as an escape from

apoptosis. This process requires increased intracellular calcium, changes in membrane lipid asymmetry, and cytoskeleton reorganization.

The clearance of circulating erythrocyte-derived microvesicles (ErMVs) occur mainly through interactions with specific receptors and

related molecules, in the monocyte-macrophage system, including liver Kupffer cells, lungs, and bone marrow.