LIFE12 ENV/IT/000352
BIONAD“Naturalised dyes replacing commercial
colorants for environmentally friendly leather dyeing and water recycle”
Start: 01/01/2014End: 30/06/2016
07/08/201518-month meeting
INESCOP premises
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
The University of Florence
Department of Chemistry “Ugo Schiff”
Organic Chemistry
The University of Florence
Laboratory No 8 Prof. Roberto BianchiniRoom 161
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
B6 : naturalised dyes in leather dyeing at pilot scale level (M13-M21)
C2 : depuration of dyeing effluents using bacteria at laboratory level (M3-M9)
C3: monitoring of quality improvement for leather dyed with nat. dyes (M13-M21)
C4 : depuration of dyeing effluents at pilot scale level (M19-M30)
C6: monitoring of quality assessment for leather dyed with nat. dyes (M13-M18)
ACTIONS OF THE EITHGTEEN MONTHS
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
•Determination of COD and BOD
•Recycling of bacterial biomass
•Action of bacterium strain E. coli on dye effluents
•Considerations on the mechanism of action of E. coli•Assessment of dye reduction (visible analysis)
•Potential reuse of dyeing effluent after depuration
Laboratory scale ; period: 3 - 9 monthsDepuration of dyeing effluents using E. coli
UNIFI
Action C.2
Extension of action requested in the amendment
Use of fungi cultures for the degradation of dyes
Selection of highperforming bacteria
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Recaps
Action C.2
ShakerE. coli: DH5 Centrifuge
Shaking at 37 C
Separation of the biomass
Nutrient only
After E. coli growth
Mixed dyes
Selection of bestperforming E. coli.
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Action C.2 Depuration of dyeing effluents using white rot fungi
Laboratory scale; period 3-9 monthsUNIFI
●The fungus used was Funalia trogii
●Eukaryote type microorganism
●More complex metabolism compared to bacteria
●Wide pool of enzymes, responsible for degradation
●Greater adaptability to the environment compared to bacteria
●Wide range of substrates to target
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Action C.2
F. trogii growing in MEA F. trogii liquid growth in BRM
Shaking at room temperature (10 d) F. trogii after liquid growth
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Action C.2
Blending the fungus F. trogii in CZAPEK Autoclave sterilizing dyes
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Action C.2
Adding the fungus to dyes
F. trogii with dye solutions
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
Ongoing resultsAction C.2
LIFE12 ENV/IT/000352 “BIONAD“ Naturalised dyes replacing commercial colorants
for environmentally friendly leather dyeing and water recycle
DR202 DB27
Mix 4 dyes
