Light Delivery Systems for Optogenetics - AMS Technologies · 2014. 6. 4. · 3 . Light Delivery...

Light Delivery Systems for OptogeneticsMIGHTEX Product Catalog and Selection Guide 2013

Close-up of a „light addressable“ fruit-fl y brain, Credit: Jay Brooks

OPTICAL TECHNOLOGIES

Light Delivery Systems for Optogenetics

Table of Content

Introduction ····················································································································································· 3

Mightex’s Optogenetics Light Delivery Systems Overview ····················································································· 3

(1) Fiber-Coupled LED System for in vivo Optogenetics

(2) Polygon400 Patterned Illumination System for ex vivo or in vitro Optogenetics

Mightex’s LED Sources Portfolio ························································································································· 5

LED Controllers ················································································································································ 6

(1) LED Controller Product Summary and Selection Guide

(2) SLA-1000-2 Manual and Analog LED Controller

(3) SLC-MA04-MU Manual and Software LED Controller

(4) SLC-AA02-US and SLC-AA04-US LED Controllers with Software and TTL Triggering

(5) BLS-Series BioLED Light Source Control Modules

Fiber-Coupled LED Sources ······························································································································ 10

(1) Fiber-Coupled LED Product Summary and Selection Guide

(2) FCS-Series Single-Wavelength Fiber-Coupled LEDs

(3) WFC-Series Multi-Wavelength Fiber-Coupled LEDs

Lightguide-Coupled LED Sources ······················································································································ 12

(1) Single-Wavelength Lightguide-Coupled LED Sources

(2) Multi-Wavelength Lightguide-Coupled LED Sources

Polygon400 Spatiotemporal Patterned Illuminators for Optogenetics ····································································· 14

(1) Polygon400 Overview

(2) Polygon400 With Built-In LED’s

(3) Polygon400 With External Light Sources

(4) Microscope Adapters for Polygon400


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Introduction Optogenetics is a technology that allows targeted, fast control of precisely defined events in biological systems, by delivering optical control signal at the speed (millisecond scale) and with the precision (cell type–specific) required for biological processing. Mightex has developed a range of Light Delivery Systems for optogenetics, as described below.

Mightex’s Optogenetics Light Delivery Systems Overview

(1) Fiber-Coupled LED System for in vivo Optogenetics

Fiber-coupled LEDs are capable of precisely delivering light to specific cell(s) of interest, without disturbing other cells in the vicinity. Moreover, multi-wavelength fiber-coupled LEDs enable one to switch wavelengths (e.g. 470nm and 590nm) of the light delivered to the cell(s) through the same fiber, without the need of any mechanical movement or to physically switch fibers. This will allow fast excitation and fast inhibition of the cell(s). Mightex’s FCS- and WFC-series fiber-coupled LED sources are designed for such in vivo optogenetics applications.

Control Signals Manual Analog TTL Software

- customer defined.

LED Controllers SLA-1000-2 SLC-MA04-MU BLS-SAxx-US

Fiber-Coupled LED’s FCS-series

(Single LED)

WFC-series

(Up to 4x LEDs) (Up to 8x LEDs)

- with single or multiple LED’s coupled into one (1) output fiber, without any moving parts in the optical path. Up to 8x LED’s can be coupled into the same fiber.

Interchangeable fiber (e.g. 50/100/200µm) Standard SMA Connector

Rotary Joint

Fiber-Optic Cannula



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(2) Polygon400 Patterned Illumination System for ex vivo or in vitro Optogenetics

For ex vivo or in vitro Optogenetics, microscopes are usually used in order to achieve the spatial resolution required to view individual cells, and patterned illuminators are needed in order to precisely control (i.e. activate/silence) the cells of interest without disturbing other cells within the microscope’s Field of View (FoV). Mightex’s Polygon400 Dynamic Spatial Illuminators are designed for such ex vivo and in vitro optogenetics applications.

Control Signals

TTL Software/PC

Polygon400 Patterned Illuminators

Microscope Adapters

DSI-x-xxx...

For Upright Microscopes

DSI-CUBE-xx-UA

For Inverted Microscopes

DSI-RING– xx

Light Sources

LEDs Lasers Others

Coupling Mechanism

Integrated/ Built-in

Lightguide Fiber

LED Controller

BLS-PLxx-US

Φ ~ mm Φ ~ 100m

TTL USB

Polygon400 on an Upright Microscope

Polygon400 on an Inverted Microscope

590nm

470nm

*

* A BLS-PLxx-US LED controller is only needed when an external LED source is used with the Polygon400. Integrated/built-in LEDs have their own integrated LED controllers.

Key Features: DLP based Patterned Illuminator;

Simultaneous illumination of multiple areas of interest;

Customer-defined shape/size of illumination pattern;

Up to 4000 frames per second;

USB2.0 interface, easy to install;

TTL trigger input/output;

Adapters for microscopes;

Built-in LEDs, or external light sources via fiber or lightguide;

Intuitive/powerful software for spatial/temporal/spectral control.




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Mightex’s LED Sources Portfolio As far as optogenetics applications are concerned, LEDs are able to simultaneously meet both the speed (~ms) and the spatial preci-sion (i.e. cell/neuron specific) requirements. Compared with lasers, LEDs excel in many respects: they are cheaper, smaller, more reli-able, and easier to control. In addition, there are a wide range of LED wavelengths available in the market, making it easy to find the best wavelengths to excite or inhibit neurons infected with various optogenetics tools, such as 470nm for channelrhodopsin (ChR2) and 590nm for halorhodopsin (NpHR).

Mightex has developed the most comprehensive LED source solutions in the market. The chart below shows the wavelength portfolio of Mightex’s LED’s.

In order to satisfy the specific needs of various customer applications, Mightex offers an extensive range of beam formats with our LED solutions, including collimated, uniform, focused, divergent, fiber-coupled, and lightguide-coupled LED sources. Microscope adapters are also available, in order to enable customers to mount the LED sources onto their microscopes. Please refer to the diagram below on how to choose the best beam format (and microscope adapters) for your specific applications.

If you require multiple wavelengths for your application, Mightex also offers a range of multi-wavelength LED solutions, as below: (1) Wavelength-switchable LED's with collimated output beam (WLS-series). With an optional lightguide adapter, the output beam can also be coupled into a liquid lightguide; (2) Multi-wavelength fiber-coupled LED's (WFC-series); and (3) Multi-wavelength collimated LED's using beam combiners. With an optional lightguide adapter, the output beam can also be coupled into a liquid lightguide. Various microscope adapters are also available for customer who would like to couple the LED light into micro-scopes.



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LED Controllers In optogenetics research, scientists may have the need to control LED’s in one or more of the following ways: (1) manually (e.g. with a turning knob); (2) with TTL pulses; (3) with analog signals; and (4) with software. Mightex has developed a range of LED controllers with different methods of control, as described in details below:.

(1) LED Controller Product Summary and Selection Guide

SLA-1000-2 SLC-MA04-MU BLS-SA02-US / BLS-SA04-US SLC-AA02-US / SLC-AA04-US

Control Signals

Number of Channels (per device) 1 2 4 2 / 4 2 / 4

Minimum Pulse Width ~1ms 1ms 20s 20s

Accepts TTL Input to Encode Output Pulse Pattern No No Yes Yes

Accepts TTL Input to Trigger Pre-defined Pulse Pattern No No Yes Yes

Manual Control via Turning Knobs Yes Yes No No

Accepts Analog Input Control Yes No No No

Software control via GUI / SDK No Yes Yes Yes

Support Arbitrary Output Waveform/Pattern Via external analog input (0~5V)

Via custom program based on SDK

Easily definable via Mightex’s software GUI / SDK

Easily definable via Mightex’s software GUI / SDK

Keys: Manual Analog TTL Software

Note: (1) Each channel is capable of independently controlling an LED or a string of LEDs (all in sync with each other). For example, with a four (4)-channel LED controller, one can control up to four (4) LED’s INDEPENDENTLY. If however one needed to control more than four (4) LED’s, multiple LED controllers can be operated in parallel.

(2) SLA-1000-2 Manual and Analog LED Controller

SLA-1000-2 LED controller has two (2) channels, and each can be operated in one of the following two modes:

- Manual Mode: The output current (in CW) can be adjusted manually via a turning knob, ranging between 0 and the Maximum Output Current stated below; - Analog Input Control Mode: The output current can be controlled via a 0~5V analog input. The maximum modulation frequency is ~1KHz.

Each channel has a three (3) digit DIP switch (as shown in the picture on the right): the 1st digit is used to set the working mode, while the 2nd and 3rd digits are used to set the Maxi-mum Output Current, which allows one to limit the maximum current that passes through the LED to match its current rating and prevent LED damage due to overdriving. There are three (3) current limit settings: 350mA, 500mA and 1000mA.

PART NUMBER AND ORDERING INFORMATION

SLA-1000-2

DIP Switches

1 2 3

A B

DIP Switch Setting Instruction

A: Control Mode Setting B: Current Limit Setting

1 Manual Mode

1 Analog Input Control Mode

2 3

2 3

2 3

Imax = 350mA

Imax = 500mA

Imax = 1000mA

Note: SLA-1000-2 is a UNIVERSAL LED controller designed to drive LED’s of any made and model. It is NOT limited to just Mightex’s LED’s.

Parameters SLA-1000-2

Number of Channels 2

Maximum Output Current (Settable via DIP Switches) 350mA / 500mA / 1000mA

Power Supply (provided by Mightex, 12V) Input Voltage, Vdc 9 ~ 24 V

Maximum Output Voltage (Vdc - 3.0) V

Analog Input Control Signal 0~5 V

Dimensions 80mm (L) x 64.3mm (W) x 23.7mm (H)

Weight 60g



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(3) SLC-MA04-MU Manual and Software LED Controller

Mightex's SLC-MA04-MU four-channel universal LED controller is designed to drive a broad range of LED light sources, and it offers the flexibility for users to operate each LED channel independently, both manually and through software. The software comes with a user-friendly GUI that enables one to drive LEDs without the need to write any code. Furthermore, a full-featured SDK is provided in order for users who wish to write their own code and to integrate the LED controller into their own systems.


SLC-MA04-MU

To PC

1. Manual Mode: Each of the four channels can be operated manually in CW mode using one of the four (4) “Channel” knobs. The LED controller also has a fifth knob (i.e. a “Global” knob) that enables one to adjust the output current of all the channels at the same time and with the same step size. Therefore, one can first set the intensities for the LED’s - independently - using the four “Channel” knobs, and then can increase/decrease the set intensities of all channels simultaneously using the “Global” knob. In order to prevent LED damage due to overdriving/overheating, the maximum output current of each LED channel can be set individually, via the software provided with the LED controller, to match the current rating of the LED connected to the channel.

2. Software Mode: In software control mode, each channel can be individually configured by the software to operate in one of the following three modes:

a. Disable Mode: The channel is disabled, and its output is completely turned off. b. Normal Mode (or CW Mode): The output current is constant, which can be adjusted (using software) from 0mA to 1,200mA through the USB interface. c. Strobe Mode: A Pulse-Width-Modulated (or PWM) periodic strobe pattern is output from the channel, which can be activated by a software trig-ger. The strobe pattern may repeat indefinitely or for a preset number of cycles, depending on the software setting. The maximum frequency of the PWM strobe is 500Hz.

Parameters SLC-MA04-MU

Number of Channels 4

Maximum Output Current (Settable via software) Up to 1200mA

Power Supply (provided by Mightex, 12V) Input Voltage, Vdc 12 ~ 24 V


Minimum Pulse Width 1ms

Timing Resolution 0.1ms

Output Current Resolution 1mA

Output Current Accuracy ±5 mA or ±1.0%, whichever is larger

Output Current Repeatability ±2 mA or ±0.5%, whichever is larger

Interface USB2.0

Device Per Computer* 16

Dimensions 180.5mm (L) x 180mm (W) x 34.5mm (H)

Weight 400g

Note: SLC-MA04-MU is a UNIVERSAL LED controller designed to drive LED’s of any made and model. It is NOT limited to just Mightex’s LED’s.

* The LED controller’s software enables up to sixteen (16) SLC-MA04-MU LED controllers to be operated simultaneously by a single computer, leading to a maximum of 64 channels in total.



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(4) SLC-AA02-US and SLC-AA04-US LED Controllers with Software and TTL Triggering

Mightex's SLC-AA02-US and SLC-AA04-US universal LED controllers are designed to drive a broad range of LED light sources, and they enable one to operate each LED channel independently through software with a user-friendly GUI. Furthermore, a full-featured SDK is provided in order for users to write their own software and to integrate the LED controller into their own systems.


SLC-AA02-US or SLC-AA04-US

To PC

Parameters SLC-AA02-US / SLC-AA04-US

Number of Channels 2 / 4

Maximum Output Current (Settable via Software) 1000mA in CW mode 3500mA in strobe mode

Power Supply (provided by Mightex, default 12V) Input Voltage, Vdc 12 ~ 24 V


Minimum Pulse Width 20s

Timing Resolution 20s

Output Current Resolution 1mA

Output Current Repeatability ±1mA (or +/-0.2%)

Interface (Settable via a Flip Switch on the Back Panel) USB2.0 or RS232

Arbitrary Waveform Definition Up to 128 points of [current (mA), time interval (s)]

Accepts TTL Trigger Yes

Device Per Computer* Up to 16

Dimensions 201mm(L) x 147mm (W) x 40mm (H)

Weight 600g

Note: SLC-AA02-US and SLC-AA04-US are UNIVERSAL LED controllers designed to drive LED’s of any made and model. They are NOT limited to just Mightex’s LED’s.

* The LED controller’s software enables up to sixteen (16) SLC-MA04-MU LED controllers to be operated simultaneously by a single computer, leading to a maximum of 32 channels for SLC-AA02-US and 64 channels for SLC-AA04-US, respectively.

The SLC-AA02-US and SLC-AA04-US LED controllers can also be controlled via TTL trigger pulses. There are two trigger modes (which are settable/selectable via the software): (1) one can pre-define a pulse sequence (or pattern) in software and load/save it to the LED controller’s non-volatile memory, and a TTL pulse can then be used to trigger/activate the pre-defined pulse pattern; or (2) one can pre-set the output current (or the LED’s intensity), and then use a sequence of TTL pulses to ‘encode’ (the timing of) the LED’s output pulse pattern. Since in the latter case, the LED’s output pulse pattern strictly follows the input TTL pulse patter, it is also called ‘Follower Mode’. With an SLC-AA02-US or an SLC-AA04-US LED controller, one can use up to 128 pairs of [current (mA), du-ration (μs)] data points to define the 'shape' of the waveform. This will allow one to define an 'Arbitrary Wave-form' for the LED driving current and consequently the LED's optical output. Details see diagram below.

RS232

USB



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(5) BLS-Series BioLED Light Source Control Modules

Mightex BLS-series BioLED light sources are modularized fully-customizable turn-key solu-tions for optogenetics, fluorescence excitation, and other biophotonics applications. Pre-cisely-timed and high-intensity light pulses are required in optogenetics experiments to acti-vate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.


BLS-SA02-US, BLS-SA04-US, BLS-PL02-US, and BLS-PL04-US

(Front View)

(Back View)

(Back Panel)

All standard Mightex LED light sources can be integrated into the BLS-series light source system. Furthermore, customers may choose optical heads with different wavelengths and formats to be integrated with the same control module. Multiple control modules can be ‘stacked’ in software to support more than 4 optical heads. The control module features a linear LED driver design that eliminates light intensity ripples and oscillations often observed when low-cost buckpuck nonlinear drivers are used. Clean and highly repeatable pulses are critical to quantitative experiments. Both CW mode and pulse modes are supported. Time resolution of the control module is 20s and light intensity can be adjusted with 0.1% increments. Each driving channel on the control module has its own TTL trigger input. Rising edge, falling edge, and follower mode are supported in the trigger mode. The control module can be operated without being connected to a computer. Once pulse sequences are programmed and stored into the control module (by user through software), the light source can operate alone without a computer. All it needs is a TTL trigger signal to output the user-programmed pulse sequences. Mightex BioLED light sources come with a Windows-based operation software featuring an intuitive yet powerful graphic user interface. A software development kit (SDK) is also pro-vided for user integration into environment such as Labview and Matlab.

BLS-SA02-US BLS-SA04-US BLS-PL02-US BLS-PL04-US

Synchronization with Polygon No Yes

Number of channels 2 4 2 4

Output Intensity Resolution 0.1%

Time Resolution 20s

External Trigger TTL

Trigger Connector BNC

Optical (LED) Head Connector 2-pin Aero Connector

Host Interface USB2.0 or RS232, selectable

On-Device Memory Yes

Device Per Computer* Up to 16

Dimensions 213mm (L) x 156mm (W) x 73mm (H)

Weight 800g

* The LED controller’s software enables up to sixteen (16) BioLED controllers to be operated simultaneously by a single computer, leading to a maximum of 32 chan-nels for BLS-SA02-US and 64 channels for BLS-SA04-US, respectively.

BLS-PLxx-US BioLED control modules are specifically designed to synchronize LED’s with Mightex’s Polygon400 Patterned Illu-minators, in order to support synchronization between external LED’s with custom pre-defined illumination patterns using external TTL trigger pulses.



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Fiber-Coupled LED Sources Mightex has developed two (2) catogories of fiber-coupled LED sources: (1) FCS-series single-wavelength fiber-coupled LED’s; and (2) WFC-series multiwavelength fiber-coupled LED’s. This section describes the fiber-coupled LED selection guide as well as the detailed specifications of the LED sources.

(2) FCS-Series Single-Wavelength Fiber-Coupled LEDs Mightex FCS-series single-wavelength fiber-coupled LED light sources employ the latest high-power LED technologies and a proprietary coupling optics to achieve maximum optical output power. Optical output is coupled into a fiber through a standard SMA fiber adaptor port (SMA fiber patch cords are sold separately). FCS series also features a locking electrical connector for secured connection. The one-piece machined housing features multiple mounting holes. All Mightex LED controllers can be used to drive the FCS-series light sources.

Interchangeable fiber SMA connector

(1) Fiber-Coupled LED Product Summary and Selection Guide

Features FCS-Series

WFC-Series

Number of Wavelengths/LEDs 1 Up to 8 Wavelength Range (nm) 240~940nm, or white LEDs 240~940nm, or white LEDs Output Fiber Interchangeable (e.g. 50/100/200µm etc.) Interchangeable (e.g. 50/100/200µm etc.) Fiber Connector SMA SMA

Note: For wavelength availability, please refer to Mightex’s LED wavelength portfolio.


For example, FCS-0470-000 is a 470nm fiber-coupled LED with an SMA fiber connector.

FCS - -XXX

Wavelength Code (Refer to table below)

Configuration Code (default: “000”)

Output power measured here!

Peak Wavelength (nm) 1 Description Wavelength Code Output Power (mW) 2 Intensity (mW/mm^2) 365 UV 365nm 0365 4.6 146 385 UV 385nm 0385 4.6 146 400 400nm 0400 5.1 162 420 420nm 0420 2.0 64 455 Royal Blue 0455 5.5 175 470 Blue 0470 6.3 201 505 Cyan 0505 2.7 86 530 Green 0530 1.6 51 590 Amber 0590 0.9 29 617 Red-Orange 0617 5.1 162 625 Red 0625 5.1 162 656 Deep red 0656 5.1 162 680 Deep Red 0680 1.1 35 740 NIR 0740 2.8 89 780 NIR 0780 1.7 54 850 NIR 0850 3.1 99 870 NIR 0870 1.1 35 940 NIR 0940 3.1 99

For optogenetics applications, light intensity (i.e. power per unit area, in mW/mm2) is a more important measure than light power (mW) itself. The table below summarizes the output power (mW) and the intensity mW/mm^2) of typical Mightex’s FCS-series single-wavelength fiber-coupled LED’s.

Note: (1) Other wavelengths and white LED’s are also available; (2) Output power measured at the output tip of a 200µm 0.39NA fiber. Higher output power can be obtained under certain conditions and in “Intellipulsing” mode with a BLS-SA02-US or a BLS-SA04-US BioLED control module. Please email [email protected] or call +1-925-218-1885 for details.

Related Products:

LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US Fiber Patch Cords: FPC-0200-22-01SMA, FPC-0200-22-02SMA, FPC-0200-37-01SMA, FPC-0200-37-02SMA



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(3) WFC-Series Multiwavelength Fiber-Coupled LEDs Mightex’s WFC-series multi-wavelength fiber-coupled light sources are enabled by the latest LED technologies and Mightex’s proprietary beam combining/coupling optics. Up to

eight (8) LEDs are coherently combined into a single multi-mode fiber with the highest efficiency practically possible. Optical output is coupled into a fiber through a standard SMA fiber adaptor port, and customers can use fibers with different core diameters with the WFC module. Each LED in the module can be powered individually or simultaneously, making the WFC-series a new class of light sources with a tunable spectrum. In optogenetics, for example, one might want to launch 470nm to excite ChR2 in a neu-ron, and then fast (in sub-ms) switch to 590nm to activate NpHR in order to inhibit the same neuron. This can be achieved by using a WFC-H2-0470-0590-000 which enables sub-ms switching between 470/590nm through the same fiber, and this can all be done electronically (through software) and there is no need for any mechanical movement.


Output power measured here!

Note: (1) Other wavelengths and white LED’s are also available; (2) Output power measured at the output tip of a 200µm 0.39NA fiber. Higher output power can be obtained under certain conditions and in “Intellipulsing” mode with a BLS-SA02-US or a BLS-SA04-US BioLED control module. Please email [email protected] or call +1-925-218-1885 for details.

Up to 4x LED’s Up to 8x LED’s

WFC - X N - XXXX - XXXX - ... XXXX - XXX

S - Standard configuration H - High-power configuration

Number of wavelengths

1st Wavelength code

2nd Wavelength code

… (repeat if

applicable)

N-th Wavelength code

Internal use

For example, WFC-H4-0400-0470-059-0656-000 is a high-power configuration WFC light source with 4 wavelengths of 400nm, 470nm, 590nm, and 656nm.

Wavelength1 (nm)

Wavelength Code

Output Power 2 (mW) / Intensity (mW/mm^2)

2-wavelength 3-wavelength 4-wavelength 5-8 wavelength

365 0365 3.8mW (120mW/mm^2) 3.6mW (115mW/mm^2) 3.4mW (108mW/mm^2) 3.1mW (98 mW/mm^2)

385 0385 3.8mW (120mW/mm^2) 3.6mW (115mW/mm^2) 3.4mW (108mW/mm^2) 3.1mW (98mW/mm^2)















Related Products: LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US Fiber Patch Cords: FPC-0200-22-01SMA, FPC-0200-22-02SMA, FPC-0200-37-01SMA, FPC-0200-37-02SMA



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Lightguide-Coupled LED Sources

Mightex GCS-series high-power LED sources are designed for high-efficiency coupling of LED light into a liquid lightguide or a fiber optic bundle. Virtually all lightguides with core diameters ranging from 3mm to 8mm can be used with the GCS-series light source. GCS series are designed as a universal light source for general lab use and OEM applications. Multi-chip LED emitters have been added to the product portfolio (Type-B), and some of these 7W to 15W Type-B LEDs have total output optical power quadrupling the power of a single-chip LED (Type-A). Type-B models feature a cooling fan, and have a different form factor compared to Type-A models. Power supply for the cooling fan is included in the price of the Type-B collimated LED sources, while Type-A LED’s do not need a cooling fan.

Type-A

Type-B

Part Number1 Wavelength Code Type Description Peak Wavelength (nm) Iop (mA) Vop (V) Radiant Flux2 (mW)

GCS-0365-02-xxxxx 0365 A UV 365nm, 2W 365 500 3.8 90 GCS-0365-07-xxxxx 0365 B UV 365nm, 7W 365 500 15 300 GCS-0385-02-xxxxx 0385 A UV 385nm, 2W 385 500 3.8 100 GCS-0385-07-xxxxx 0385 B UV 385nm, 7W 385 500 15 330 GCS-0455-04-xxxxx 0455 A Royal Blue, 4W 455 1000 3.9 165 GCS-0470-04-xxxxx 0470 A Blue, 4W 470 1000 3.9 130 GCS-0470-15-xxxxx 0470 B Blue, 15W 470 1000 15 400 GCS-0505-04-xxxxx 0505 A Cyan, 4W 505 1000 3.9 30 GCS-0530-04-xxxxx 0530 A Green, 4W 530 1000 3.9 60 GCS-0530-15-xxxxx 0530 B Green, 15W 530 1000 15 180 GCS-0590-03-xxxxx 0590 A Amber, 3W 590 1000 3.2 35 GCS-0617-03-xxxxx 0617 A Red-Orange, 3W 617 1000 3.0 200

GCS-0617-10-xxxxx 0617 B Red-Orange, 10W 617 1000 10.8 600

GCS-0625-03-xxxxx 0625 A Red, 3W 625 1000 3.0 200 GCS-0850-02-xxxxx 0850 A NIR, 2W 850 1000 2.1 125

GCS-0940-02-xxxxx 0940 A NIR, 2W 940 1000 2.4 125

GCS-3400-04-xxxxx 3400 A Warm White, 4W 3400K 1000 3.9 95

GCS-5500-04-xxxxx 5500 A Cool White, 4W 5500K 1000 3.9 95

GCS-6500-04-xxxxx 6500 A Glacier White, 4W 6500K 1000 3.6 95

GCS-6500-15-xxxxx 6500 B Glacier White, 15W 6500K 1000 15 300

Table-1: Performance Specifications


For example, GCS-0470-15-A0510 is a 15W 470nm light guide coupled LED source with a standard adapter for Mightex 3mm liquid lightguide (with 5mm ferrule diameter).

GCS - - XXXXX

Wavelength Code (Table-1)

Lightguide Adapter Code

(Table-2)

-

Electrical Power (W)

Note: 1. “xxxxx” is the Lightguide Adapter code, see Table-2 below; and 2. Measured at exiting end of a 3mm-core 0.59 numerical aperture (NA) liquid light guide.

Table-2: Lightguide Adapters Adapter Code For Ferrule Diameter (mm) For Ferrule Length (mm) A0510 5 ≥10

A0610 6 ≥10

A0710 7 ≥10

Related Products:

LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US

(1) Single-Wavelength Lightguide-Coupled LED Sources



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(2) Multi-Wavelength Lightguide-Coupled LED Sources A Multi-Wavelength Lightguide-Coupled LED Source can be constructed using the following Mightex’s parts: (1) LCS-series colli-mated LED sources with wavelengths of customer’s choice; (2) appropriate Mightex’s LCS-BCxx series multi-wavelength beam com-biners; (3) connecting plates that bind the beam combiners together; and (4) a lightguide adapter that focuses a collimated beam into a lightguide. For details, please see schematic diagram below.

Beam Combiner

Connecting Plates Lightguide Adapter

Lightguide

An LCS-series collimated LED consists of a collimating lens and a LED emitter. The LED emitter is placed at the focal plane of the collimating lens which images the LED emitter into infinity. The LED emitter is mounted directly onto the metal base of the collimator which also features an integrated heat sink. The light sources can be driven by Mightex LED controllers, or other LED controllers and current sources. An optional focusing module can be mounted on the front of the LED collimator to focus light into a tight spot which is an image of the LED emitter. One of the applications with the focusing module is to couple LED light into a fiber or a light guide.

A four (4) wavelength lightguide-coupled LED source based on LCS-series collimated LED’s.

Mightex’s LCS-BCxx series multi-wavelength beam combiners can be used to combine two LCS-series colli-mated LED’s of different wavelengths into a single collimated beam. Multiple combiners can be cascaded to combine more than two collimated LED sources. At the heart of the beam combiner is a high-performance dichroic mirror that combines two wavelengths with >95% efficiency. Beam combiners are characterized by the edge wavelengths of their dichroic mirrors. Please use the following table to select the correct beam combiner for intended LCS-series LED sources.

Part Number Reflection Port %R Transmission Port %T

LCS-BC25-0380 365nm >95 385, 455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, 850, 870 or 940nm >95

LCS-BC25-0400 365 or 385nm >95 455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, 850, 870 or 940nm >95

LCS-BC25-0410 365, 385, or 400nm >95 455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, or 850nm >95

LCS-BC25-0460 400nm >95 470, 505, 530, 590, 617, 625 or 657nm >95 LCS-BC25-0480 365, 385, 455, or 470nm >95 505, 530, 590, 617, 625, 657, 680, 740, 780, or 850nm >95 LCS-BC25-0495 400, 455 or 470nm >95 530, 590, 617, 625 or 657nm >95 LCS-BC25-0505 455 or 470nm >95 530, 590, 617, 625, 657 or 740nm >95 LCS-BC25-0515 365, 385, 455, 470, or 505nm >95 530, 590, 617, 625, 657, 680, 740, 780, or 850nm >95 LCS-BC25-0520 455, 470 or 505nm >95 590, 617, 625, 657 or 740nm >95 LCS-BC25-0550 365, 385, 455, 470, 505, or 530nm >95 590, 617, 625, 657, 680, 740, 780, or 850nm >95 LCS-BC25-0560 470, 505 or 530nm >95 590, 617, 625, 657 or 740nm >95 LCS-BC25-0595 505 or 530nm >95 617, 625, 657, 740 or 850nm >95 LCS-BC25-0605 365, 385, 455, 470, 505, 530, or 590nm >95 617, 625, 657, 680, 740, 780, or 850nm >95 LCS-BC25-0635 470, 505, 530, 590, 617, or 625nm >95 657, 740, or 850nm >95 LCS-BC25-0660 590, 617 or 630nm >95 740 or 850nm >95 LCS-BC25-0685 590, 617, 630 or 657nm >95 740 or 850nm >95

LCS-BC25-0800 455, 470, 505, 530, 590, 617, 625, 657, 680, 740, or 780nm >95 850, 870 or 940nm >95

LCS-BC25-0810 740 or 780nm >95 850 or 940nm >95

LCS-BC25-0000 400, 455, 470, 505, 530, 590, 617, 630, 657, or white ~45 400, 455, 470, 505, 530, 590, 617, 630, 657, or white ~45

LCS-BC25-0070 400, 455, 470, 505, 530, 590, 617, 625, 657, or white ~30 400, 455, 470, 505, 530, 590, 617, 625, 657, or white ~70

LCS-series collimated LED sources



14


Polygon400 Spatiotemporal Patterned Illuminators for Optogenetics

Spatially patterned illumination with temporal and spectral control enables numerous new techniques in life-science applications such as optogenetics. For example, in optogenetics, selected neurons in a specimen slice can be activated or silenced with a user de-fined illumination pattern. Mightex Polygon400 multi-wavelength patterned illuminator integrates the state-of-the-art spatial light modulators and high-power LEDs using a proprietary Etendue-preserving optical design to deliver high-intensity illumination patterns with diffraction-limited reso-lution. A Texas Instruments’ DLP spatial light modulator is used to display a user defined image pattern. At the heart of the Poly-gon400 is a unique optical system that carefully delivers light from LED sources or an external light source (such as a laser or an arc lamp, throught an optical fiber or a lightguide) to the DLP panel and then through a microscope to the specimen plane. Such a sys-tematic approach makes it possible to achieve maximum optical intensity while maintaining diffraction-limited imaging performance. Temporal performance is a key to many intended applications for Polygon400. With a frame rate of more than 4,000 fps and fast-switching LEDs, Mightex Polygon400 can deliver illumination patterns with micro-second precision. Advanced DSI models with ex-ternal LED controllers also enable wavelength switching in between illumination patterns at the highest frame rate. A dedicated software allows users to generate illumination patterns as well as control illumination intensity and timing. The software also supports alignment between illumination patterns and images acquired through any digital cameras on a microscope.

Projection Area Common Microscopes

Leica Nikon Olympus Zeiss Height (mm) 8.7 8.7 7.8 7.2 Width (mm) 15.5 15.5 13.9 12.7 Diagonal (mm) 17.7 17.7 16.0 14.6

Pixel size (m) 18.0 18.0 16.2 14.8

Projection Area (FOV) and Pixel Resolution (at 1X magnification)

Note: To calculate illumination area and pixel resolution at the specimen, simply divide the above numbers by the magnification of the objective. For example, under a 10x objective on an Olympus microscope, the illumination area will be 0.78mm x 1.39mm with a pixel resolution of 1.62m.


DSI- x- xxxx- xxxx- xxxx- xxx

I - with built-in LEDs

G - with lightguide input

F - with fiber input

1st wavelength code

2nd wavelength code

3rd wavelength code

Reserved for internal use

Not applicable for lightguide/fiber based Polygon models

For example, DSI-I-0470-0590-000 is a Polygon400 module with two built-in LED sources: 470nm and 590nm. DSI-G-000 is a Polygon400 module with a lightguide as input light source.

(1) Polygon400 Overview

Control and Timing

Parameters Minimum Maximum Unit Allowed bitmap depth 1 8 bit Exposure time @ 1bit 0.25 2,000 ms Frame rate @ 1bit - 4000 1/sec Exposure time @ 4bit 1.6 20 ms Frame rate @ 4bit - 625 1/sec Exposure time @ 8bit 8.3 20 ms Frame rate @ 8bit - 120 1/sec Input trigger TTL, BNC connector - Input trigger delay - 50 s Output trigger TTL, BNC connector - Output trigger delay ms User Programmable

System and Communication USB2.0 interface. 5Vdc 3A input power.

Windows 7 (recommended), Windows XP Service Pack 2 or greater Screen resolution of 1366x768 or higher

TTL Trigger-In TTL Trigger-Out



15


Mightex Polygon400 is designed to be easily inserted into the infinity path of a microscope. For inverted microscopes, the preferred inserting point is usually the back port of the microscope where a fluorescence attachment is commonly placed. A filter cube is required to fold the Polygon400’s light path into the microscope. The filter cubes used for fluorescence observation serves this pur-pose well. For upright microscopes we provide a beam combiner cube to be inserted below the binocular/trinocular unit. The dichroic or mirror in the beam combiner directs the Polygon400 beam into the microscope light path.

Leica Nikon Olympus Zeiss

Upright DSI-CUBE-LC-UA DSI-CUBE-NK-UA DSI-CUBE-OL-UA DSI-CUBE-ZS-UA

Inverted TBD DSI-RING-NK-TI DSI-RING-OL-IA TBD

Microscope Adapters for Polygon400

(Left - Polygon400 beam combiner cube for upright microscopes; and, right - adapter ring for inverted microscopes.)

1. These are estimated power at specimen under a 20x 0.75NA Olympus objective.

Available LED Wavelengths and Output Power on the Specimen Under Microscope (in Pattern Sequence Mode) Wavelength (nm) Output Power1(mW)

400 14.0 470 15.0 530 4.5 590 3.0 617 14.0 630 14.0 656 14.0

(2) Polygon400 With Build-In LED’s A Polygon400 unit may contain up to three (3) built-in LED’s, each of which can be operated sequentially (in Color Image Mode) or individually (in Pattern Sequence Mode). In this case, LED controllers are integrated into the Polygon400’s control electronics residing inside the metal housing, and hence there is no need for external LED controllers.

(3) Polygon400 With External Light Sources

(4) Microscope Adapters for Polygon400

Polygon400 can also work with external light sources (e.g. lasers, arc lamps, and exter-nal LED sources etc) through a lightguide or an optical fiber. Two additional models of Polygon400 have been developed for this purpose: one (i.e. Polygon400g) with an light-guide input port, and the other (i.e. Polygon400f) with a fiber input port.

Polygon400 (standard)

Polygon400g P/N: DSI-G-000

Polygon400f P/N: DSI-F-000

Light Source Input Port N.A. (integrated LED’s) Lightguide Optical Fiber

LED as Light Sources Yes Yes Yes

Laser as Light Sources No Yes Yes

Hg Arc Lamp No Yes No

Other Light Sources No Yes No

Different Light Sources for Polygon400

Polygon400g (P/N: DSI-G-000)

Lightguide P/N: LLG-03-59-340-0800-1

LED#1 (LCS-xxxx)

LED#2 (LCS-xxxx)

LED#3 (LCS-xxxx)

LED#4 (LCS-xxxx)

BioLED Control Module P/N: BLS-PL04-US

Mightex Multi-Wavelength Lightguide-Coupled LED



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