Development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-

streaked dwarf virus and rice ragged stunt virus

Zhuo Chen1,2 • Qin Guo1,2• Bing-Hua Chen1,2• Xiang-Yang Li2• Zhen-Chao Wang2• Peng He2• Fei Yan1• De-Yu Hu2•Song Yang2

1 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Hangzhou 310021 P.R. China 2 State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, P. R. China

The program of mass spectrometer (MS) about protein identification was set as follow: the

identification of proteins polypeptides was conducted using 5600 Triple TOF MS (Foster City,

CA, USA), the condition was descripted as follow, Gas 1 and Gas 2 of Ion Source Gas: 18 and 0,

Curtain Gas: 30, IonSpray Voltage Floating: 2300 V, collision energy (CE): 14, Interface Heater

Temperature: default, Scan type: positive, TOF MS: 350-1250, Accumulation time: 0.25 sec,

calibration molecular weight of compounds from mobile phase (CH-2) in TOF-MS: 371.10123

and 445.12002, Product Ion: 100-1500, Product Of: 391.3. The Beta Galactosidase Digest (LC/MS

Peptide CA, Foster City, CA, USA) was selected to the protein standard in auto calibration, the

program of auto calibration was set as follow: the time of acquiring data: 30 min, Accumulation

time in TOF-MS: 0.25 secs, TOF Masses (Da): 350-1800, Polarity: Positive, duration: 30 min,

Product Of: 729.36, TOF Masses (Da): 100-1800, High Sensitivity, Duration: 30 min, Cycles:

3273, Collision Energy (CE): 45.0, the injection amount was set to 2 μL.

The program of MRM was set by description as follow: “For ions greater than” and “For ions

smaller than” were 400 m/z and 1250 m/z, “With charge state” and “Which exceeds” were from

“2” to “5”, “Mass Tolerance” was “50”, Maximum number of candidate ions to monitor per cycle:

3, “Exclude former target ions” was “Always” and for “3” seconds, the molecular weight of 3

precursor ions was selected in the window of “Include List”, the retention time (RT) (mins) were

set 15.0, “For” was set to “900”.

1

Fig. S1 The pattern nucleonic acid and protein of separation against rice protein (a), SRBSDV P9-1 gene (b) and

P9-1 protein (c). a: the separation of total rice protein using 12% SDS-PAGE. The letter of “M” represented Page

RulerTM Prestained Protein Ladder, and lane 1-2 represent the rice sample; b: the separation of pET28a-P9-1 and

P9-1 using 1% agarose gel electrophoresis. The letter of “M” represented the standard marker of nucleinic acid; c:

the separation of expressed P9-1 using 12% SDS-PAGE. The letter of “M” represented Page RulerTM Prestained

Protein Ladder, lane “1-3” represented the protein product of expressed P9-1.

2

Fig. S2 The digestion map of polypeptides of southern rice black-streaked dwarf virus (SRBSDV) P9-1 and its

information of precursor ions and the product ion of polypeptides fragments. In a. the sequence with bold format

labeled with red or green represent the polypeptides sequence by trypsin, the sequence was numbered using Arabic

numerals in the bracket over the horizontal line. In b. the No. was agreement with the number of sequence in (a).

The value in (b) represent the value of the precursor ions and the product ion of polypeptides fragments.

3

Fig. S3 The digestion map of polypeptides of rice ragged stunt virus (RRSV) P3 and its information of precursor

ions and the product ion of polypeptides fragments. In a the sequence with bold format and labeled with red or

green represent the polypeptides sequence by trypsin, the sequence was numbered using Arabic numerals in the

bracket over the horizontal line. In b the No. was agreement with the number of sequence in (a). The value in (b)

represent the value of the precursor ions and the product ion of polypeptides fragments.

Part 1

A. SRBSDV P2

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

Inte

nsity

0

20

40

60y13+2b3 b4 y10+2b5

y9y8y7y6y5y4b12+2

y2y1

1025.57175.12342.21 411.21

1137.54560.21 813.41

630.33 894.41 1210.69 1513.181397.68470.23

1349.68 1449.62 1616.88 1736.91770.33 856.52

Contrib 2.00, Conf 99, Sequence DLNNIQLNFYDTR, ∆Mass 0.0031, Prec MW 1624.7877, z 2,

Sc 10

B. SRBSDV P3

m/z, Da

100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950

Inte

nsity

0

200

400 y8+2b2y7

b3y6

b4+2y5b5

y4b6

y3y3+2 b7

y2y1 219.148713.416584.371

120.081 323.135 695.376 812.476247.142 480.287386.234 528.318212.103436.229 733.358 846.450260.198

610.286129.099

659.797

Contrib 2.00, Conf 99, Sequence FVEPNLIK, ∆Mass -0.0015, Prec MW 958.5473, z 2, Sc 12

4

C. SRBSDV P5

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

Inte

nsity

0100200300400

y12+2b2 b3

y10

b4

y9

y9+2

y8

y8+2

y7y6y5y4

b10

y3

y3+2

y2y1 956.50

316.15226.11 339.20519.25

618.291069.59

731.37136.07429.24175.12 859.45681.33 1108.61

765.39629.27 992.54262.13 925.47

Contrib 2.00, Conf 99, Sequence DSILPQIVNEEK, ∆Mass 0.0019, Prec MW 1383.7264, z 2, Sc

12

D. SRBSDV P6

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

Inte

nsity

0200

400600

800b2 y12+2b3 y11+2b4

y10y10+2

y9y9+2

y8y8+2

y7y7+2 b8

y6y5y4y3y2y1

221.13 648.35 934.50249.12 524.29 819.471047.57372.26

301.15 499.60 618.40563.25121.08 732.45 917.46

431.20 1194.661030.54

Contrib 2.00, Conf 99, Sequence AFTFLDSNVFKPK, ∆Mass 0.0006, Prec MW 1512.7980, z 3,

Sc 15

E. SRBSDV P7-1

m/z, Da

0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300

Inte

nsity

0

100

200

300b4 b5 b6 b7y10+2 b8

y9b9

y8b10b10+2

y7y6y5y4y3y2 333.19 1017.59

718.43

171.11

708.36

300.15448.21413.23 960.59120.08

676.31426.231143.52 1219.59 1351.55

Contrib 2.00, Conf 99, Sequence MLTALSLGNAFISEGR, Modifications Oxidation(M)@1, ∆Mass -

0.0018, Prec MW 1694.8645, z 3, Sc 14

F. SRBSDV P9-1

m/z, Da

0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

Inte

nsity

0

50

100

150 b2 b3y16

b8y15y14

y14+2y12y11y10y9y8y7y6y5y4y3y2y1

473.30

572.36395.19

985.54328.10

1227.66799.50214.11 1699.921187.64

1340.81671.29102.06 837.40 1469.771114.65

1598.871301.67 1682.85

Contrib 2.00, Conf 99, Sequence ESEESLTEEILEGEGAVVNVLK, ∆Mass 0.0041, Prec MW z 2373.1833, Sc

2

G. SRBSDV P10

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

Inte

nsity

0

20

40

60

80

y18+2b2 b3 b4y15

b5y14

b6y13

b7y12y11y10y9y8y7y6y5y4y2y1

1122.62

1027.00830.48446.27129.111251.70

543.30 1494.76639.33300.12 802.32 1448.65960.52 1505.73 1694.66877.30

Contrib 2.00, Conf 99, Sequence AKDEDQDEYELTGLSALR, ∆Mass 0.0007, Prec MW 2051.9653, z 2,

Sc 18

5

Part 2

A. RRSV P1

m/z, Da

0 100 200 300 400 500 600 700 800 900 1000 1100 1200

Inte

nsity

0

500

1000

1500y11+2b2 b3

y9b4

y8y7y7+2

y6y6+2

y5y4y3y2b11+2

y1764.38 952.46

1065.56157.13 185.13865.43120.08 803.50468.28 625.31298.21 554.28 1029.52382.69 746.36266.15 1091.51

Contrib 2.00, Conf 99, Sequence AIISTPISYER, ∆Mass -0.0030, Prec MW 1248.6685, z 2, Sc 12

B. RRSV P2

Contrib 2.00, Conf 99, Sequence AIGSFVPSLPTSGTR,∆Mass -0.0020, Prec MW 1488.7916, z

2, Sc 16

C. RRSV P3

m/z, Da

100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000

Inte

nsity

0

1000

2000

b2 b3y8

y8+2b4y7

y7+2 b5y6

b6y5

b7y4

b8y3

y3+2y2y1

741.497

286.140187.071

458.306 571.391345.219 629.398159.077 779.528258.146 840.574399.219

Contrib 2.00, Conf 99, Sequence DAVLGLLAVR, ∆Mass 0.0064, Prec MW z 1025.6296, Sc 2

D. RRSV P4a

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300

Inte

nsity

05

1015202530354045

y13+2b2 b3

y11

y11+2b4

y10y9y8y7y6y5y4y3649.35

738.35185.12

637.33936.44 1000.43

783.43 1113.52557.26 682.35378.19 1049.50263.07 885.39

468.27 551.32567.27244.09 277.69 371.22 841.38 894.44777.29 1095.50 1123.42 1280.34716.36

Contrib 2.00, Conf 99, Sequence ALLDMTGTTSSGK, ∆Mass -0.0028, Prec MW 1296.6202, z

2, Sc 11

E. RRSV P7

6

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

Inte

nsity

0

20

40

60y15+2b2 b3

y13b4

y12y11b6

y10y9y8y7y6y5y4y1

228.13 980.43794.36665.29 1180.54136.08 493.24299.18 437.20 783.46567.27

893.40

941.42355.151067.52752.39 1267.61 1339.68 1464.63186.09

1023.47 1395.68855.21

Contrib 2.00, Conf 99, Sequence LNASSLSVEGDNFGR, ∆Mass 1.0055, Prec MW 1565.7537, z

2, Sc 15

F. RRSV P8

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

Inte

nsity

0

50

100 b2 b3 b4 b5y13

b6y12

b7y11

b8b8+2y10

b9y9

b10y8

b11b11+2y7

y7+2y6

b13y5y4y3y2y1

717.37143.12 830.42343.15 929.53602.34375.22577.28186.09 617.26 845.37 1114.60356.19 1342.721227.63

Contrib 2.00, Conf 99, Sequence GIANYDINAVLDNIIDK, Modifications Cleavages ∆Mass -

0.0031, Prec MW 1859.9598, z 3, Sc 23

G. RRSV P9

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

Inte

nsity

0

10

20

30

40y19+2b3 b4 b5

y15y14y14+2b7

y13y12y11y10y9y8y7y7+2

y6y5y5+2

y4y3b18+2

y2y1136.07 1121.59

262.14563.29 1582.81

348.181639.83

450.191284.66 1469.65

879.45764.44618.36 1008.47201.12

749.37 1309.641061.56830.43 1181.54994.55 1752.931524.721693.87

Contrib 2.00, Conf 99, Sequence ATYNIGLGQYLEDEALLYR, ∆Mass -0.0052, Prec MW

2201.0955, z 2, Sc 19

H. RRSV P10

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

Inte

nsity

0

50

100b2 b3

y10b4

y9y8b6+2

y7y6b8b8+2

y5y5+2 b9

y4y3b11

y2y1

304.09 403.16276.10

1166.61980.53764.47 1265.68879.49

538.29159.09 651.38451.27229.13 785.401395.721148.60

937.521448.76368.12

728.32

Contrib 2.00, Conf 99, Sequence CEVWTDLLSYLR, Modifications Carbamidomethyl(C)@1;

Methyl(E)@2, ∆Mass 0.0028, Prec MW 1567.7733, z 2, Sc 15

I. RRSV non-structual protein

m/z, Da

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

Inte

nsity

0

50

100y14+2b3 y12+2b4

y11b5

y10b7

y8y7y6y5y5+2

y4b12+2

y3b13

y2y1324.12 1092.54

772.86120.08 306.12 456.21425.17

567.27 654.26278.15

909.47 1160.52679.82205.14 1221.58791.42365.16 1074.56 1313.551491.70

Contrib 2.00, Conf 99, Sequence ADHEPIDISSAMMK,Cleavages cleaved E-A@N-term, ∆Mass 0.0002,

Prec MW 1543.7013, z 2, Sc 14

Fig. S4 The spectrum of MS/MS about southern rice black-streaked dwarf virus (SRBSDV) P9-1 and rice ragged

7

stunt virus (RRSV) P3. Part 1 and 2 represent the spectrum of protein of SRBSDV or RRSV. The word of

‘‘contrib’’ in figure indicate the contribution of this peptide and the highest value is ‘‘2’’. The word of ‘‘conf’’ in

figure indicate the confidence for the peptide identification, and the value is expressed as a percentage. ∆Mass in

figure indicate the difference in mass between the precursor MW and the theoretical MW of the matching peptide

sequence. Prec MW z in figure indicate the ratio of precise molecular weight and charge of peptides. Sc (Score) in

figure indicate the score for the peptide which is based on matching ions of various charge states.

Fig. S5 The spectrum about the polypeptides of m/z 599.3174 about southern rice black-streaked dwarf virus

(SRBSDV) P9-1. ‘‘Mass/Charge, Da’’ represent the ratio of molecular and charge about detected peptides, and is

shown ‘‘m/z’’. m/z 599.3174 indicated the precursor ions in a figure, and m/z 821.49, 708.40 and 637.37 indicated

the product ion of the precursor ions (m/z 599.3174) in b figure.

8

Fig. S6 The spectrum about the polypeptides of m/z 716.4051 about SRBSDV P9-1. m/z 716.4001 indicated the

precursor ions in a figure, and m/z 925.567, 838.5386, 725.4642 and 612.3793 indicated the product ion of the

precursor ions (m/z 716.4051) in b figure .

Fig. S7 The spectrum about the polypeptides of m/z 1187.6255 about SRBSDV P9-1. m/z 1187.6255 indicated the

precursor ions in part a in figure, and m/z 1469.8414, 1340.7076 and 1227.6872 indicated the product ion of the

precursor ions (m/z 1187.6255) in part b in figure.

9

Fig. S8 The spectrum about the polypeptides of m/z 896.5058 about SRBSDV P9-1. m/z 896.5058 of the MW

indicated the precursor ions in part a in figure, and m/z 1116.6340, 1003.5455, 916.5042 and 815.4623 indicated

the product ion of the precursor ions (m/z 896.5058) in part b in figure.

Fig. S9 The spectrum about the polypeptides of m/z 629.8721 about rice ragged stunt virus (RRSV) P3.

‘‘Mass/Charge, Da’’ represent the ratio of molecular and charge about detected peptides, and is shown ‘‘m/z’’. m/z

629.8721 indicated the precursor ions in a figure, and m/z 1,046.5849, 878.4964, 731.4243 and 630.3721 indicated

the product ion of the precursor ions (m/z 629.8721) in b figure.

10

Fig. S10 The spectrum about the polypeptides of m/z 471.7850 about RRSV P3. m/z 471.7850 indicated the

precursor ions in part a in figure, and m/z 744.4226, 745.4273, 615.3818 and 516.3133 indicated the product ion of

the precursor ions (m/z 471.7850) in part b in figure.

Fig. S11 The spectrum about the polypeptides of 891.4578 about RRSV P3. m/z 891.4578 indicated the precursor

ions in part a in Figure, and m/z 1126.5397, 1039.5275, and 952.4847 indicated the product ion of the precursor

ions (m/z 891.4578) in part b in figure.

11

Fig. S12 The spectrum about the polypeptides of m/z 779.3809 about RRSV P3. m/z 779.3809 indicated the

precursor ions in part a in figure, and m/z 970.4421, 899.4184, and 784.3977 indicated the product ion of the

precursor ions (m/z 779.3809) in part b in figure.

Fig. S13 The spectrum about the polypeptides of m/z 1140.1195 about RRSV P3. m/z 1140.1195 indicated the

precursor ions in part a in figure, and m/z 1431.8090, 1317.7636, 1205.6721, 1133.6414 and 595.3871 indicated

the product ion of the precursor ions (m/z 1140.1195) in part b in figure.

12

Fig. S14 Detection of SRBSDV and RRSV by the assays of simplex RT-PCR and duplex RT-PCR using virus

specific primer pairs. a: the PCR product of 242 bp indicated S10 of SRBSDV; b: the PCR product of 395 bp

indicated S8 of RRSV; c: the results in lane “1-3” indicated that co-infection of SRBSDV and RRSV, the results in

lane “4-6” indicated that the rice sample infected by RRSV, the results in lane “7-9”; d: Simultaneous detection of

SRDSBV and RRSV using duplex RT-PCR against the infected rice plants collected from Yunnan Province using

the duplex RT-PCR method. Letter of “M” in Part (a-d) indicated DNA marker (TAKARA, Dalian,

China).indicated that the rice sample infected by SRBSDV. Letter of “H” in figure. indicated the healthy sample

un-infected by SRBSDV or RRSV.

Table S1 the setting of flowrate and component of CH-1 in LCTime (min)

Qa (μL/min) Qa (μL/min)

0 2 010 2 0

Table S2 the setting of flowrate and component of CH-2 in LCelution program of 30 min

elution program of 120 min

Time (min) %A %B Time (min) %A %B0 95 5 0 95 513 60 40 60 75 2515 40 60 85 55 4515.5 20 80 95 40 6020 20 80 100 5 9520.5 95 5 110 5 9530 95 5 110.1 95 5- - - 120 95 5

Table S3 The components and ratio of proteins between BSA, OVA and SRBSDV P9-1.

Treatment No. 1 2 3 4 5 6 7 8 9

13

Protein content (μg)

BSA/OVA 00.

51.0 1.50

1.75

01.875

1.937

51.96875 2.

P9-1 21.

51.0 0.5 0.25 0.125

0.062

50.03125 0.00000

Total 2 2 2 2 2 2 2 2 2

Table S4 Some protein coded by SRBSDV and RRSV genome using shotgun proteomics.

SRBSDV RRSV

Protein name

Experimental location of

protein detected (group)

The predicted molecular weight (kDa)/Theoretical

location (group/Accession)

Protein name

Experimental location of

protein detected (group)

The predicted molecular weight (kDa)/Theoretical

location (group/Accession)

P1 1, 2, 3 1/168.9/AFR68771 P1 1, 2, 3, 8 1/137.7

P21, 2, 3, 4, 5,

6, 81/141.1/AFR68772 P2

1, 2, 3, 4, 5, 6, 7

1/133.1

P3 3 1/135.4/AFR68773 P31, 2, 3, 4, 5,

6, 7, 8,1/130.8

P4 1, 8 1/131.7/AFR68774 P4a 3, 4, 5 1/141.4P5-1 1, 2, 3, 4, 5, 6 2/108.3/AFR68775 P4b Non 6/36.9P5-2 Non 23.7/ AEV41415 P5 Non 3/91.4

P61, 3, 4, 5, 6,

7, 83/89.9/AFR68776 P6 Non 4/65.6

P7-1 6 6/40.5/AFR68777 NS7 4, 5, 8 4/68.0

P7-2 Non 6/36.5/AFR68778 P81, 2, 3, 4, 5,

6, 7, 84/67.3

P8 Non 4/67.9/AFR68779 P8a Non 8/25.6P9-1 1, 3, 5, 6, 7 6/39.8/AEB40349 P8b Non 6/41.7P9-2 Non 8/24.2/AEB40350 P9 6, 7, 8 6/38.6

P101, 3, 4, 5, 6,

7, 84/62.5/AEB40351 NS10

1, 2, 3, 4, 5, 6, 7, 8

7/32.3

Table S5 The information of identification protein using Proteinpilot software.Unused Total %Cov Accession# Name Species Peptides%25.43 25.4

375.8 gi|

429128495P2 SRBSDV 13

4.05 4.05 59.4 gi|336454497

P3 SRBSDV 2

36.27 36.2 65.9 gi| P5 SRBSDV 23

14

7 42912850122.97 22.9

762.0 gi|

404434733P6 SRBSDV 16

2.38 2.38 54.5 gi|348652179

P7-1 SRBSDV 1

33.03 33.03

84.2 gi|379995844

P9-1 SRBSDV 23

24.24 24.24

95.3 gi|320332272

P10 SRBSDV 17

20.32 20.32

84.1 gi|330688226

P1 RRSV 11

15.55 15.55

75.0 gi|330688228

P2 RRSV 10

53.65 53.65

70.9 gi|81922309 P3 RRSV 30

8.51 8.51 75.5 gi|81984743 P4a RRSV 75.522.72 22.7

275.7 gi|

330688216P6 RRSV 20

2.3 2.3 84.7 gi|330688238

NS7 RRSV 1

14.16 14.16

62.6 gi|330688220

P8 RRSV 10

12.15 12.15

87.3 gi|330688242

P9 RRSV 8

14.02 14.02

63.3 gi|42433259 NS10 RRSV 10

15

Table S6 The information about polypeptides using common method of the protein identification and Proteinpilot software.Protein name Contrib Confidence Sequence Modifications Cleavages ∆Mass Prec MW z Sc Spectrum Type

SRBSDV P10 2 99 AKDEDQDEYELTGLSALR 0.0007 2051.9653 2 18 1.1.1.3086.24 Winner

2 99 EVPAQMGILTDEVK -0.0041 1528.7767 2 15 1.1.1.3130.22 Winner

2 99IDGGYDFNCPASTTDVTHYGGY

DQYSR

Carbamidomethyl(C)@9;

Asp->Glu@150.0097 3072.2932 3 14 1.1.1.3075.14 Winner

2 99 IGEILSAIWK -0.0022 1128.6521 2 15 1.1.1.3637.3 Winner

2 99 IHFPVQYFDEVK 0.0014 1520.7678 3 14 1.1.1.3277.4 Winner

2 99 LDIAPDLIHNGVPQR 0.001 1656.8956 2 12 1.1.1.2976.14 Winner

2 99 LDKIDDWSLIAK missed K-I@3 -0.0012 1415.7649 3 18 1.1.1.3126.2 Winner

2 99 LIITSVLPNIIQAVYK 0.0002 1784.0814 2 16 1.1.1.3982.6 Winner

2 99 LQTVETSTLPNITQLK -0.0018 1784.9866 3 17 1.1.1.3230.8 Winner

2 99 TDPSNNVMNSVIISR -0.0012 1645.8082 2 18 1.1.1.2935.15 Winner

1.85 98.8LSDTIILNNRPTITLLSHFNSLFHE

SNIVK0.0206 3435.8721 5 9 1.1.1.3857.7 Winner

1.8 98.7 AYHLAPVIMK 0.0009 1141.6327 3 15 1.1.1.2593.2 Winner

0.24 99 AKDEDQDEYELTGLSALRK missed R-K@18 -1.0272 2179.0322 3 13 1.1.1.2858.7 Winner

0.09 22.3 TIHVFEKELSALDADK -0.0141 1814.9271 3 7 1.1.1.3245.6 Winner

0.09 97.4 YIKLGIEKcleaved D-Y@N-term; missed K-

L@3-0.07 962.5101 2 13 1.1.1.2489.14 Winner

0.05 97.7 ALAAPCIDYIKLGIEKAla->Ser@3;

Carbamidomethyl(C)@6missed K-L@11 0.0152 1789.9801 2 10 1.1.1.4029.3 Winner

0.04 9.3 GSSLRKTEIEDK missed R-K@5; missed K-T@6 0.0064 1361.7214 2 4 1.1.1.3603.8 Winner

0.04 9.3 TKAKDEDQDEYELTGLSALR missed K-A@2 0.033 2281.1404 3 5 1.1.1.3729.4 Winner

0.01 9.2 LSDTIILNNR cleaved R-P@C-term -0.0035 1157.6368 2 5 1.1.1.3185.2 Winner

16

0.01 9.3 FIEWYSKTFAK 　 missed K-T@7 -0.0173 1418.7063 2 5 1.1.1.3562.14 Winner

SRBSDV P5-1 2 99 DSILPQIVNEEK 0.0019 1383.7264 2 12 1.1.1.3380.8 Winner

2 99 DSILPQIVNEEKAEFDR missed K-A@12 0.0026 2002.0032 3 14 1.1.1.3789.8 Winner

2 99 EPIFQNFDISLK -0.0033 1449.7471 2 13 1.1.1.3887.12 Winner

2 99 FSTQMSYNWR -0.0004 1318.576 2 13 1.1.1.3176.16 Winner

2 99 LGNPLAPNYK -0.0008 1085.5863 2 16 1.1.1.2769.4 Winner

2 99 LSLLHDNLLLK -0.003 1277.7678 2 15 1.1.1.3499.12 Winner

2 99 SSAFQPFK -0.0009 910.4541 2 12 1.1.1.2797.6 Winner

2 99 TGFNALLTK -0.0038 963.5351 2 13 1.1.1.3043.8 Winner

2 99 TIESILEEVSDAFVANYHK 0.0008 2164.0696 3 19 1.1.1.4296.2 Winner

2 99 TLFISHPILR -0.0039 1195.7039 2 12 1.1.1.3118.13 Winner

2 99 TTSYVDKVTNNDVK missed K-V@7 -0.0025 1582.7814 3 15 1.1.1.2566.6 Winner

2 99 VGVDYSNGELSYDISSR 0.0001 1859.8539 2 15 1.1.1.3364.21 Winner

2 99 VGWNLVDIDNMGSDDNLNR Oxidation(M)@11 0.0018 2161.9714 2 12 1.1.1.3680.12 Winner

2 99 VGYIDGISR -0.0009 978.5125 2 14 1.1.1.2765.9 Winner

2 99 VGYIDGISRDELIGASGK missed R-D@9 -0.0056 1848.9526 2 14 1.1.1.3303.21 Winner

2 99 VLFNPTNEIR -0.0002 1201.6455 2 13 1.1.1.3171.9 Winner

2 99 VVTEDDMIHFGR 0.0031 1417.6692 3 13 1.1.1.3092.4 Winner

1.3 95 DAITIDPTK -0.0005 972.5123 2 10 1.1.1.2655.5 Winner

0.59 82.9 QEYENLFKK Gln->pyro-Glu@N-term missed K-K@8 -0.002 1180.5745 2 10 1.1.1.3195.10 Winner

0.1 20.5 QEYENLFK 0.0002 1069.5083 2 8 1.1.1.3006.3 Winner

0.08 16.5 KVLFNPTNEIR missed K-V@1 -0.0093 1329.7312 3 9 1.1.1.2899.2 Winner

0.07 14.7ILNHEQADNTLLTTLMIIHDFED

THTR0.018 3190.5896 5 8 1.1.1.4147.4 Winner

0.04 9.6 WELFTR 0.0002 850.4339 2 7 1.1.1.3315.2 Winner

17

0.04 9.5 DELIGASGKK missed K-K@9 -0.0116 1016.5387 2 5 1.1.1.3112.7 Winner

0.04 9.5 FAKRKPYFR missed K-R@3; missed R-K@4 -0.0072 1211.6854 2 7 1.1.1.3092.13 Winner

SRBSDV P9-1

2 99 DFNIASLYVR 　 　 -0.0065 1196.6124 2 10 1.1.1.3808.4 Winner

2 99 ENFEICTDQLDLANYVRCarbamidomethyl(C)@6;

Asp->Glu@8　 0.0024 2112.9814 2 16 1.1.1.3988.17 Winner

2 99 ESEESLTEEILEGEGAVVNVLK 　 　 -0.0044 2373.1753 3 20 1.1.1.4236.9 Winner

2 99 FQLSSLISTPTSILER 　 　 0.0114 1790.9894 3 14 1.1.1.4138.3 Winner

2 99 FVEMSLLPLLNR 　 　 -0.0022 1430.7932 2 15 1.1.1.4128.10 Winner

2 99 GFLMHLGENPNSYDR 　 　 0 1748.7941 2 16 1.1.1.3228.20 Winner

2 99 GINGSESFLNIK 　 　 -0.0004 1277.6613 2 14 1.1.1.3282.19 Winner

2 99 GINGSESFLNIKR 　 missed K-R@12 -0.0048 1433.7579 2 14 1.1.1.2998.20 Winner

2 99 IEELTIR 　 　 -0.0007 872.4961 2 10 1.1.1.2736.2 Winner

2 99 IEELTIRNDQPTR 　 missed R-N@7 -0.0046 1583.8221 3 11 1.1.1.2633.7 Winner

2 99 INHIYYQLATFDNYPFDLLR 　 　 0.0129 2515.2664 3 11 1.1.1.4167.10 Winner

2 99 IVGNADSVIK 　 　 -0.0006 1014.5705 2 12 1.1.1.2587.3 Winner

2 99 KFVEMSLLPLLNR Oxidation(M)@5 missed K-F@1 -0.001 1574.8846 3 12 1.1.1.3659.4 Winner

2 99 NTNLSLSQSTENR 　 　 -0.0012 1462.7001 2 15 1.1.1.2554.6 Winner

2 99 SDFSSLKLDV 　 missed K-L@7 0.0013 1109.5616 2 14 1.1.1.3520.2 Winner

2 99 YRPLLVSIVGYEYLVGTTVPEK 　 　 0.0053 2495.3728 3 10 1.1.1.4150.15 Winner

1.02 99 RGINGSESFLNIK Deamidated(N)@4cleaved F-R@N-term; missed R-

G@1-0.0076 1434.739 3 14 1.1.1.3156.4 Winner

0.01 2.5 QTVLFR 　 　 -0.0135 762.4253 2 5 1.1.1.2830.4 Winner

SRBSDV P6 2 99 AFTFLDSNVFKPK 　 　 0.0006 1512.798 3 15 1.1.1.3665.2 Winner

2 99 DKAFTFLDSNVFKPK 　 missed K-A@2 -0.006 1755.9137 3 16 1.1.1.3545.8 Winner

2 99 FLWQLSSE 　 　 -0.0003 1008.4915 2 11 1.1.1.3855.3 Winner

18

2 99 FSEQSENVLQFIDAQPNSQLLINK 　 　 -0.0001 2761.3921 3 15 1.1.1.4093.11 Winner

2 99 HNEACIIQNNEEILNLR

Carbamidomethyl(C)@5;

Methyl(I)@6;

Deamidated(Q)@8

　 0.0135 2094.0293 2 13 1.1.1.3398.16 Winner

2 99 LNEAIDELEKAEDR 　 missed K-A@10 -0.0023 1643.7981 2 18 1.1.1.3157.12 Winner

2 99 LSEENGQLLNAIK 　 　 -0.0007 1427.7612 2 14 1.1.1.3157.7 Winner

2 99 NMTYEDFVTNAK Oxidation(M)@2 　 0.002 1447.6311 2 12 1.1.1.2961.16 Winner

2 99 QLDDAYSELVK Gln->pyro-Glu@N-term 　 -0.0073 1262.5957 2 13 1.1.1.3682.14 Winner

2 99 SWADVVER 　 　 -0.0005 960.4661 2 12 1.1.1.2959.3 Winner

1.57 98.8 TSSIALNDVIK 　 　 0.0085 1159.6533 2 12 1.1.1.3134.17 Winner

1.33 97.9 LFVVFQK 　 　 0.0011 879.5229 2 10 1.1.1.3273.2 Winner

0.03 14.4 LIEFAR 　 　 -0.0011 747.4269 2 8 1.1.1.2729.2 Winner

0.03 13.7 ALIKFDSQK 　 missed K-F@4 0.003 1048.5947 2 4 1.1.1.3887.2 Winner

0.01 98.5 DAFEAKLQEANK Asp->Gly@1 missed K-L@6 0.0241 1304.6967 2 11 1.1.1.3453.8 Winner

0.01 3 TLEVQEQQR 　 　 0.0106 1129.5835 2 6 1.1.1.2732.6 Winner

SRBSDV P2 2 99 DLNNIQLNFYDTR 　 　 0.0031 1624.7877 2 10 1.1.1.3668.13 Winner

2 99 DNVDHEYINPLSNAIPK 　 　 -0.0051 1937.9432 2 8 1.1.1.3397.23 Winner

2 99 ESTSEILSTVR 　 　 -0.0005 1220.6245 2 14 1.1.1.2983.16 Winner

2 99 GYGNTDQFAAYLINLATQR 　 　 -0.0075 2115.0313 2 12 1.1.1.4213.15 Winner

2 99 ISDENIFNQFVLNR 　 　 0.0009 1707.8589 3 16 1.1.1.3981.2 Winner

2 99 LSAFWTTSNGR 　 　 -0.0022 1238.6021 2 12 1.1.1.3148.10 Winner

2 99 NDTVTTTGSFLSR 　 　 0.0051 1397.6837 2 12 1.1.1.2943.16 Winner

2 99SQEKTDSVDPDAVINQEGITNSS

RPEEIK　 missed K-T@4 -0.0125 3185.5203 4 13 1.1.1.3106.21 Winner

2 99 TDSVDPDAVINQEGITNSSRPEEI 　 　 0.0071 2713.3113 3 13 1.1.1.3237.25 Winner

19

K

2 99 TKPLSIEYDNTLISK 　 　 -0.0059 1720.9189 3 14 1.1.1.3152.18 Winner

2 99 VISHVTFDDDLPLFR 　 　 -0.0003 1772.9095 3 18 1.1.1.3813.7 Winner

2 99 YPFFETVVR 　 　 -0.0018 1156.5898 2 9 1.1.1.3521.5 Winner

1.17 96.4 LPILDIR 　 　 0.0003 838.5281 2 10 1.1.1.3331.2 Winner

0.09 29.8 EASMINAVNTR 　 　 0.0141 1204.6013 2 10 1.1.1.2654.4 Winner

0.03 13.7 AHIIFNGIYHSVTPFQK 　 　 0.0103 1971.0469 3 5 1.1.1.3847.4 Winner

0.03 13.7 INTVFTIFNYLHK 　 　 0.003 1608.8695 3 5 1.1.1.4077.2 Winner

0.03 12.1 YISLSYR 　 　 0.0143 900.4849 2 8 1.1.1.2839.5 Winner

0.02 9.5 DKKMFSR 　 missed K-K@2; missed K-M@3 -0.0026 910.4669 2 5 1.1.1.3089.2 Winner

0.02 9.5 FIIQNRTDELLDR 　 missed R-T@6 -0.0118 1631.8513 3 7 1.1.1.3317.10 Winner

0.02 9.5 NLFGQNDIGVK 　 　 -0.0092 1203.6157 2 6 1.1.1.3135.2 Winner

SRBSDV P3

2 99 FVEPNLIK 　 　 -0.0015 958.5473 2 12 1.1.1.3007.6 Winner

2 99 LLFDSLNLK 　 　 -0.0018 1061.6102 2 11 1.1.1.3701.3 Winner

0.01 13.7 MLKALHFDK 　 missed K-A@3 0.0224 1101.6229 2 4 1.1.1.3837.3 Winner

0.01 13.7 QDNLGTNRLERR 　 cleaved L-Q@N-term; missed R-

L@8; missed R-R@11-0.0074 1470.7579 2 4 1.1.1.3955.7 Winner

0.01 9.5 EYGAEIAASGHFIK 　 　 0.0036 1491.7393 3 6 1.1.1.3029.6 Winner

0.01 9.5 IGLFMRNK 　 missed R-N@6 -0.0144 977.5337 2 5 1.1.1.3339.3 Winner

0.01 7.8 RGITVIK 　 missed R-G@1 -0.0124 785.5001 2 7 1.1.1.2753.5 Winner

SRBSDV NS7

2 99 MLTALSLGNAFISEGR Oxidation(M)@1 　 -0.0018 1694.8645 3 14 1.1.1.3929.3 Winner

0.37 90.7 IDLFQGIR 　 　 -0.0064 960.5329 2 8 1.1.1.3486.3 Winner

0.01 13.7 NEIQDVRIYK 　 missed R-I@7 0.0162 1276.6938 2 5 1.1.1.3950.8 Winner

RRSV P3 2 99 CEVDVEAPNNALPLDLR Carbamidomethyl(C)@1;

Methyl(E)@2

　 0.0033 1937.9548 2 16 1.1.1.3352.15 Winner

20

2 99 DAVLGLLAVR 　 　 0.0057 1025.629 2 15 1.1.1.3511.3 Winner

2 99 DIGMPLAYLR 　 　 -0.0016 1147.6042 2 12 1.1.1.3573.3 Winner

2 99 DLNQTGQLVSK 　 　 0.0008 1201.6311 2 16 1.1.1.2352.8 Winner

2 99 DYVEVEYSHDLFQVR 　 　 -0.0029 1897.8817 2 17 1.1.1.3389.20 Winner

2 99 ELLSGSLSSTEFR 　 　 -0.0012 1424.7136 2 15 1.1.1.3128.10 Winner

2 99 FASELSYSRPMSFVQGK 　 　 0.0011 1932.9415 3 14 1.1.1.3151.5 Winner

2 99 FIWPISSIDQNVVVR 　 　 -0.0033 1771.9589 2 13 1.1.1.3842.15 Winner

2 99 GDLQLIPEGLMPR 　 　 -0.0108 1437.7543 3 13 1.1.1.3723.2 Winner

2 99 IPADAGNQQIISLIR 　 　 1.0095 1608.9087 3 16 1.1.1.3347.7 Winner

2 99 ITNDVLSSAQQSYLSR 　 　 0.0005 1780.8961 2 18 1.1.1.3040.12 Winner

2 99 IVPAFTLEQIK 　 　 -0.0022 1257.7311 2 11 1.1.1.3353.11 Winner

2 99 LGLTAIADSSSEYSR 　 　 -0.0016 1568.7667 2 16 1.1.1.3021.10 Winner

2 99 LLQAFDETLTLVK 　 　 -0.0034 1489.8358 2 15 1.1.1.3756.3 Winner

2 99 LNEIEVVTGGSR 　 　 0.0004 1272.6678 2 15 1.1.1.2782.5 Winner

2 99 LTSQSAADYAFLDR 　 　 0.0011 1556.748 2 14 1.1.1.3053.18 Winner

2 99MLSPQQELSYLLVGAALSQDVY

K　 　 -0.0014 2552.3184 3 20 1.1.1.4076.2 Winner

2 99PASHFEPEKNVDDKGNVTGSAP

PVSK　 cleaved K-P@N-term; missed K-

N@9; missed K-G@140.0058 2706.3306 5 15 1.1.1.2327.8 Winner

2 99 QALGIFYQGEFYYRPALVPK 　 　 -0.0032 2359.2334 3 11 1.1.1.3706.7 Winner

2 99 RPALFTTDLSQFR 　 　 0.0023 1550.8228 3 14 1.1.1.3282.5 Winner

2 99 TEYWNPYPNPGAQHVEQTIFK 　 　 1.9941 2520.1858 3 12 1.1.1.3440.13 Winner

2 99 TNVNLVVTK 　 　 -0.0025 986.5735 2 14 1.1.1.2378.5 Winner

2 99 VGYDGTLGNSIPITDEDYWSNA

R

　 　 0.0024 2542.1633 3 15 1.1.1.3590.10 Winner

21

2 99 VISLTNGVINYTER 　 　 -0.0003 1577.8411 2 16 1.1.1.3229.16 Winner

2 99 YVENELTIIAPTPQDPPIIR 　 　 -0.0001 2278.2207 3 14 1.1.1.3609.9 Winner

1.85 98.6 YRPAYALDSY 　 　 -0.0023 1217.5695 2 12 1.1.1.2819.11 Winner

0.83 85.1 LLGLIQR 　 　 -0.0025 811.5255 2 10 1.1.1.2807.2 Winner

0.45 64.2 MYFLSR 　 　 -0.0006 815.3995 2 8 1.1.1.2795.2 Winner

0.39 59.2 IPIELR 　 　 -0.0022 739.4571 2 8 1.1.1.2555.2 Winner

0.13 25.3 ALLAYSLSGSTVDLMQSDESER 　 　 0.0343 2371.1558 3 8 1.1.1.3678.7 Winner

RRSV non-

structural

protein

2 99 ADHEPIDISSAMMK 　 cleaved E-A@N-term 0.0002 1543.7013 2 14 1.1.1.2886.11 Winner

2 99 DTAFAETGTEQASPK 　 　 0.0007 1551.7061 2 19 1.1.1.2320.8 Winner

2 99 EFVVVPNSLAVVDMDTSAWER Deamidated(N)@7 　 0.0222 2364.1528 3 17 1.1.1.3884.12 Winner

2 99 ESGVAALPDKLMLETYLR 　 missed K-L@10 -0.0037 2005.0515 3 14 1.1.1.3724.2 Winner

2 99 GNPNLIANAVSDAWANHSQISK 　 　 0.0014 2306.1418 3 18 1.1.1.3530.8 Winner

2 99 HFEDTSAMLETVSTTNAFPFR 　 　 0.0024 2400.1079 3 20 1.1.1.3720.3 Winner

2 99 LASMEMISNVKPIR 　 　 0.0004 1587.8481 3 15 1.1.1.3016.5 Winner

2 99 LIAAAFQK 　 　 -0.0026 860.5095 2 13 1.1.1.2482.8 Winner

2 99 LKESGVAALPDK 　 　 0.0022 1226.6892 3 15 1.1.1.2297.2 Winner

2 99 MQLFIVK 　 　 -0.002 877.5074 2 11 1.1.1.3130.3 Winner

2 99 NSVHVCGPLNIGAFRVal->Leu@5;

Carbamidomethyl(C)@6　 0.0007 1653.8416 3 16 1.1.1.3136.11 Winner

2 99 NWEDDNITMFFHTSNVFPSEK 　 　 0.011 2557.1328 3 13 1.1.1.3854.15 Winner

2 99 SLAVVDMDTSAWER 　 cleaved N-S@N-term 0.0067 1578.7417 2 13 1.1.1.3347.13 Winner

2 99 SSASNEILVLDINELRK 　 missed R-K@16 -0.0005 1900.0261 2 17 1.1.1.3466.8 Winner

2 99 SSDKTPIPEIR 　 missed K-T@4 -0.0038 1241.6578 2 14 1.1.1.2399.5 Winner

2 99 VLQSPQFPIHTYGDDITVFDK 　 　 -0.0049 2419.2014 3 14 1.1.1.3592.10 Winner

2 99 VLQSPQFPIHTYGDDITVFDKLR 　 missed K-L@21 0.0048 2688.3962 4 19 1.1.1.3658.4 Winner

22

2 99YTYSLFTDEPGTITLADETPPTISP

TGR　 　 0.0343 3042.5063 3 13 1.1.1.3807.10 Winner

1.92 99 TTYAVTIER 　 　 -0.0039 1052.5465 2 13 1.1.1.2473.6 Winner

RRSV P8

2 99 GIANYDINAVLDNIIDK 　 　 -0.0031 1859.9598 3 23 1.1.1.3939.3 Winner

2 99 MNPASNSILPAK 　 　 -0.0036 1241.6403 2 14 1.1.1.2529.8 Winner

2 99 SDSDLIVLSK 　 　 -0.0056 1075.5704 2 16 1.1.1.2755.11 Winner

2 99 TFHLIHQSHYTPPPNK 　 　 0.0054 1915.9745 4 15 1.1.1.2311.3 Winner

2 99 YNTTNYVVSYPDTDDGR 　 　 0.0041 1978.8586 2 19 1.1.1.2795.25 Winner

2 99 YNTTNYVVSYPDTDDGRK 　 missed R-K@17 0.0027 2106.9519 3 14 1.1.1.2638.13 Winner

1.4 98.4 WVELAVEENEIHIPFNTK 　 　 0.0035 2167.0984 3 10 1.1.1.3701.5 Winner

0.69 90.6 LEAYDDVVER 　 　 0.0031 1207.5753 2 11 1.1.1.2597.11 Winner

0.08 32.1 EVMISLR 　 　 0.0061 846.4695 2 8 1.1.1.2698.3 Winner

RRSV NS10

2 99 CEVWTDLLSYLRCarbamidomethyl(C)@1;

Methyl(E)@2　 0.0028 1567.7733 2 15 1.1.1.3983.2 Winner

2 99 FSTALSYLNAK 　 　 0.0017 1213.6361 2 14 1.1.1.3083.17 Winner

2 99 LGEVANFGDDAE 　 　 0.0004 1235.5311 2 17 1.1.1.2857.9 Winner

2 99 LPSIVGLHYSGEPTTLGGIITR 　 　 -0.0019 2280.2458 4 17 1.1.1.3637.3 Winner

2 99 MSSDIGLVYHMNR 　 　 0.0022 1521.7091 2 14 1.1.1.2871.10 Winner

2 99 PFVQFPNLFEISK 　 cleaved M-P@N-term -0.0035 1564.8254 2 18 1.1.1.3908.6 Winner

2 99 YDPAQSLVVSAFFTVK 　 　 0.0028 1770.9219 3 13 1.1.1.3923.2 Winner

0.02 12.4 QGKTVSELK 　 missed K-T@3 -0.0146 988.5406 2 7 1.1.1.2569.9 Winner

RRSV P9 2 99 ATYNIGLGQYLEDEALLYR 　 　 -0.0052 2201.0955 2 19 1.1.1.3893.11 Winner

2 99 DPFTAPATGTYGTIYASR 　 　 -0.0003 1887.9001 3 19 1.1.1.3189.5 Winner

2 99 FLEDANPEIYELSR 　 　 -0.0037 1694.8115 2 18 1.1.1.3335.22 Winner

2 99 GENANYDLVTQTFIPSDAFLK 　 　 -0.0142 2342.1289 3 14 1.1.1.3842.10 Winner

23

2 99 LAFDSHLFVNR 　 　 -0.0006 1317.6823 3 12 1.1.1.3118.2 Winner

2 99 LLLSVQNHTGLDAGLQGDSSK 　 　 0.0014 2152.1135 3 13 1.1.1.3033.9 Winner

0.12 61.2 EYWLIVK 　 　 -0.0036 949.5237 2 7 1.1.1.3259.3 Winner

0.02 15.8 WYQQTFK 　 　 -0.0055 999.4759 2 7 1.1.1.2508.11 Winner

0.01 11.4GENANYDLVTQTFIPSDAFLKDN

FK　 　 -0.0183 2846.3582 4 7 1.1.1.3829.4 Winner

RRSV P7

2 99 LNASSLSVEGDNFGR 　 　 1.0055 1565.7537 2 15 1.1.1.2941.16 Winner

2 99 VGTSGFSLNYNHPYIR 　 　 0.0026 1823.8982 3 13 1.1.1.3024.5 Winner

0.16 79.8 LLQSTLPDVEK 　 　 -0.0089 1241.6779 2 10 1.1.1.2768.6 Winner

0.04 46 LLSDGLPK 　 　 0.0065 841.4975 2 8 1.1.1.2509.4 Winner

RRSV P1

2 99 AIISTPISYER 　 　 -0.003 1248.6685 2 12 1.1.1.2866.8 Winner

2 99 YGGNVEQIGNVVSDSER Deamidated(N)@10 　 0.0112 1822.8445 2 15 1.1.1.2940.11 Winner

0.03 44 IISLEK 　 　 -0.0009 701.4315 2 8 1.1.1.2548.2 Winner

0.01 56.8 ILDLVR 　 cleaved Y-I@N-term -0.003 727.4563 2 9 1.1.1.2665.2 Winner

24

Table S7 The detection of protein from viruliferous-rice of SRBSDV and RRSV using one injection via protein MS at the mode of full scan.

SRBSDV RRSVN Unu

sedTotal

%Cov

Accession

Name

Peptides (95%)

N Unused

Total

%Cov

Accession Name Peptides (95%)

1 26.44

26.44

88.7

gi|312274958

P6 17 1 66.09

66.09

87.8

gi|81922309 P3 46

2 20.18

20.18

99.0

gi|347544067

Major capsid protein

13 2 29.02

29.02

96.8

gi|330688242

Spike protein

23

3 17.80

17.80

100.0

gi|379995844

P9-1 16 3 28.13

28.13

93.3

gi|42433259 P10 51

4 14.11

14.11

93.1

gi|404434725

P2 8 4 22.72

22.72

75.7

gi|330688216

P6 20

5 11.44

11.44

93.9

gi|429128501

P5-1 6 520.3

220.32

84.1

gi|330688226

P111

6 2.01 14.11

100.0

gi|340026131

Putative capsid protein

8 6

19.25

19.25

90.3

gi|81983918 P8

12

7 0.09 0.09

85.3

gi|312260845

P1 0 715.5

515.55

75gi|330688228

P210

8 0.08 0.08

91.3

gi|404434723

Reversed P1

0 8

8.518.5

175.

5gi|81984743

RNA-directed RNA polymerase

5

9 0.06 0.06

87.1

gi|312274960

Reversed P5

0 92.3 2.3

84.7

gi|330688238

NS71

25

10

0.05 0.05

92.6

gi|336454490

Reversed P6

0 10

0.1 0.170.

2RRRRRgi|3327940

REVERSED polymerase

0

11

0.05 0.05

92.7

gi|404434738

P8 0 11 0.08

0.08

74.2

RRRRRgi|81922309

P30

12

0.050.0

565.

8gi|3327940

polymerase

46

26