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Rotifers
their role and production requirements
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Reproduction
Size
Tolerance
Mobility
Bio-encapsulation
Mass production
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Life cycle B. plicatilis
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Tank: Any Size/Shape (2-6 m3) Temp.: 25-27 °C (Preheated Water/Heat Exchange) Salinity: 18-25 ppt Oxygen: 5-7 ppm (Pure Oxygen) pH: 7.5-8.5 NH3/NH4+: < 10 mg/l NH3: < 1 mg/l Water: Filtered (1 m), UV, Chlorinated/Neutralized Aeration - Reasonably Strong - Airstones Illumination: Low Light Conditions Floc traps Batch Culture: 3-5 days
Rotifer production – optimal culture and enrichment conditions
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Rotifer production in 3m3 tanks
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Low density rotifer culture
Sea bream
General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 10
2) Rotifer production volume/100M daily production (m3) 1,5
3) Period of rotifer feeding (days) 20
4) Culture Selco needed per 100M rotifers produced (kg) 0,2
5) Culture Selco price (drs/kg) 11234
Annual production target (2g juveniles)
No of larval batches 5 5 5 5
Initial larval rearing vol (m3) 62 123 185 308
Daily rotifer requirement (M) 615 1.231 1.846 3.077
Rotifer production volume (m3) 9 18 28 46
No of 2.8m3 tanks needed 3 7 10 16
No of 4m3 tanks required 0 0 0 0
Total rotifer production volume (m3) 8 20 28 45
Total Culture Selco requirement (kg) 123 246 369 615
Requirements 2.000.000 4.000.000 6.000.000 10.000.000
Cost of Culture Selco (Mdrs/yr) 1,38 2,77 4,15 6,91
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Rotifer culture using algae excellent results with excellent results with
algae but expensivealgae but expensive
time consuming (culture time consuming (culture of algae + counting)of algae + counting)
availability of algaeavailability of algae
quality of the rotifers quality of the rotifers dependent on quality of dependent on quality of the algaethe algae
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Rotifer culture using algae and yeast
variable results in variable results in rotifer survival rotifer survival depending on the depending on the quality of algae and quality of algae and freshness of the yeastfreshness of the yeast
unexpected mortalities unexpected mortalities (total crashes) due to (total crashes) due to lack of standardisationlack of standardisation
variability in rotifer variability in rotifer quality (nutrition, quality (nutrition, microbiology)microbiology)
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Batch culture of rotifers on algae and yeast
harvest
drain culture water
Culture period6-10 days
feeding
Re-inoculation
Live food aquaculture training coursewww.aquatrain.org
Medium density rotifer culture
Sea bream
General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 10
2) Rotifer production volume/100M daily production (m3) 0,4
3) Period of rotifer feeding (days) 20
4) Culture Selco 3000 needed per 100M rotifers produced (kg) 0,20
5) Culture Selco price (drs/kg)
Annual production target (2g juveniles)
No of larval batches 5 5 5 5
Initial larval rearing vol (m3) 62 123 185 308
Daily rotifer requirement (M) 615 1.231 1.846 3.077
Rotifer production volume (m3) 2 5 7 12
No of 2.8m3 tanks needed 3 7 10 16
No of 4m3 tanks required 0 0 0 0
Total rotifer production volume (m3) 8 20 28 45
Total Culture Selco 3000 requirement (kg) 123 246 369 615
Requirements 2.000.000 4.000.000 6.000.000 10.000.000
Cost of Culture Selco (Mdrs/yr) 0,00 0,00 0,00 0,00
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Rotifer growth comparison CS v’s CS3000CS 3000-MD vs. CS-LD
0
200
400
600
800
1000
1200
1400
1600
1800
0 1 2 3
Time (days)
Ro
tife
r d
en
sit
y (
#R
/ml)
CS 3000-MD
CS-LD
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Specific Growth Rate in MD batch culture
SGR in MD batch culture: CS vs. CS 3000
0
0.10.2
0.30.4
0.5
CS 3000 CS
SG
R (d
^-1)
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Method of determining rotifer growth and condition– mean specific growth rates
SGR = ln (Final Number)-ln (Initial number) No. of days of culture
ln = natural log
Useful for:- Following feeding experimentsQuantitatively assessing culture stability
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Rotifer production methodologiesCulture Selco 3000 and O2
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Rotifer Production
MAM2
M: Masters
A: Culture (2-6 m3)
B: Inoculum (500 R/ml)
C: Enrichment
(500-1000 R/ml)
Larval Tanks
B C
30% 70%
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Culture Selco 3000 strategy
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Traditional rotifer batch culture
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High density rotifer production system
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Rotifer harvesting
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Harvesting of rotifers
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Transfer of rotifers
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Recirculated ultra-high density rotifer culture ~5000 rotifers + /ml
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Commercially available rotifer and artemia cultivation and enrichment products
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Fatty acid enrichment levels in Rotifers
mg/g mg/gEnriched rotifers EPA DHANannochloropsis 7,3 2,2Nannochloropsis sp. 3,44 0,6Chlorella 3,75 0,6Isochrysis sp. 1,87 1,25Protein Selco 8,125 8,125Super Selco 33,12 36,25DHA Super Selco 41,4 68