1 1 © 2009 PerkinElmer © 2009 PerkinElmer © 2009 PerkinElmer © 2012 PerkinElmer
Marleen Stomp-Smit
Staff Scientist LSC
Center of Excellence for LSC Cocktails and Vials
PerkinElmer Groningen, The Netherlands
Safer, NPE-free LSC Cocktails
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Introduction
Surfactants
NonylPhenolEthoxylates (NPE’s)
AlcoholEthoxylates (AE’s)
NPE replacement challenges
New NPE-free LSC cocktail formulations
Properties
Storage stability data
Customer evaluation
Presentation Outline
New developments in Liquid Scintillation instrumentation and reagents
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LSC cocktail composition
Aromatic solvent
Scintillators
Surfactants
NonylPhenolEthoxylates (NPE’s)
AlcoholEthoxylates (AE’s)
LSC cocktail developments
Shift to safer solvents
Scintillators no change
NonylPhenolEthoxylate (NPE) surfactants recently scrutinized
Introduction
New developments in Liquid Scintillation instrumentation and reagents
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NonylPhenolEthoxylates (NPE’s)
Primarily used in domestic and industrial detergents
Effective surfactants in general
Good wetting agents, excellent emulsifying and dispersing properties
Storage and shipping classification in general
UN 3082, Environmentally Hazardous Substance
Biodegradation products endocrine disruptor (gender bender)
Decreasing availability
Gradual laundry industry phase out
AlcoholEthoxylates (AE’s)
Most common alternative for NPE’s
Less effective surfactants in general
Excellent wetting agents, moderate emulsifying and dispersing properties
Not classified as dangerous for storage and shipping
Expanding availability
Complete range
Surfactants
New developments in Liquid Scintillation instrumentation and reagents
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Take advantage of the AE’s excellent wetting properties and simultaneously
minimize the impact of moderate emulsifying and dispersing properties
Combined surfactant system
AE range, Phosphate esters, Succinates.
AE’s impact on LSC cocktails
Counting properties
Efficiency, Sample Load Capacity
Possible 14C introduction
Fatty alcohol source
Possible 40K introduction
Reaction catalyst
AE’s suitable for LSC cocktails
Synthetic alcohol source
Sodium reaction catalyst
NPE replacement challenges
New developments in Liquid Scintillation instrumentation and reagents
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Ultima Green
High-Flashpoint (Safer) NPE-free LSC Cocktail
Universal LSC cocktail
High counting efficiency
Quench resistent
Tritium (15µl Trititiated Hexadecane) Efficiency
10 ml Cocktail: 54,6%
10 ml Cocktail + 1 ml water: 49,3%
Background with NaOH
2 min count time
0,0-18,6 keV
New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
3 ml 0,1M NaOH 3 ml 1,0M NaOH
2 min. 69 5653
15 min. 12 260
30 min. 15 97
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New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
Ultima Green Sample Load capacity
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Aqua Green
High-Flashpoint (Safer) NPE-free LSC Cocktail
High aqueous sample load
Suitable for Low Level applications
Tritium (15µl Trititiated Hexadecane) Efficiency
10 ml Cocktail: 45,8%
10 ml Cocktail + 10 ml water: 20,8%
Low Level Background
120 min. Count time
Tri-Carb Low Level Mode
10 ml cocktail + 10 ml water
New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
Insta-Gel Plus Aqua Green Ultima Gold LLT
0,5-5,0 keV 2,76 2,67 1,64
0-18,6 keV 5,09 5,00 2,84
20-1400 keV 6,32 3,13 2,87
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New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
Aqua Green Sample Load capacity
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Storage stability data
tSIE (Quench Parameter) used as indicator
Average tSIE decay for Safer LSC cocktails is about 30 units per year
Ultima Green
Storage at room temperature
December 2011 tSIE 628
January 2013 tSIE 581
Storage at 30°C
December 2011 tSIE 628
January 2013 tSIE 576
New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
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Storage stability data
Two months storage at 30°C, weekly measurements
New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
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Two Safer, NPE-free LSC cocktails are developed
Customer feedback is greatly appreciated
Currently customer evaluation in progress
Qualify for beta testing at our booth!
New NPE-free LSC Cocktail formulations
New developments in Liquid Scintillation instrumentation and reagents
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Safer, NPE-free LSC Cocktails
New developments in Liquid Scintillation instrumentation and reagents
Thank you for your attention
Questions or remarks?
Please visit us at the PerkinElmer booth
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PerkinElmer Groningen LSC Cocktails and vials Production site
