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Human Hematopoietic Stem Cell CD34 CD38 low/neg CD59 CD90 (Thy1) CD117 (c-kit) CD133 CD135 (Flt3) CD164 CD338 lin neg GATA2 TdT Multi-Potent Progenitor (MPP) CD34 CD135 (Flt3) lin neg TdT Common Lymphoid Progenitor (CLP) Pro-B CD10 CD19 CD20 CD22 CD34 neg CD72 CD74 CD124 CD164 CD252 CD268 CD275 HLA-DR Pre-NK/T CD5 CD34 Pro-NK CD7 CD10 neg CD34 neg CD117 (c-kit) Immature NK CD34 neg CD94 neg CD117 (c-kit) CD122 CD161 Ets-1 Id2 Id3 NKT CD3 CD8 CD16 CD56 CD69 CD161 Vα24Jα18TCR Id2 PLZF CD4 ISP (Immature Single Positive) CD1a CD2 CD3 CD4 CD5 CD7 CD34 CD45RO CD127 (IL-7Rα) TCRβ low Early Double Positive (EDP) CD1a CD2 CD3 CD4 CD5 CD7 CD8α CD38 CD45RO CD127 (IL-7Rα) TCRβ Double Positive (DP) γδ T CD4 neg γδ TCR Sox13 CD8 CD8 Granulysin Granzyme A Granzyme B Perforin Runx3 CD4 CD4 Pre-B CD10 CD19 CD20 CD22 CD34 neg CD72 CD74 CD79a CD124 CD164 CD252 CD268 CD275 HLA-DR IgM Pax5 Immature B CD5 CD10 CD19 CD20 CD21 CD22 CD23 CD24 CD32 CD37 CD40 CD48 CD52 CD72 CD75 CD79a CD79b CD80 act CD84 CD86 act CD98 CD124 CD137 (41BB) act CD152 act CD153 CD180 CD184 CD185 CD196 CD200 CD210 CD229 CD252 act CD253 CD267 (TACI) CD275 CD279 (PD-1) CD307 CD317 (BST2) CD319 (CRACC) IgD IgG IgM Pre-T CD1a CD2 CD3 CD5 CD7 high CD28 CD34 CD45 CD127 (IL-7Rα) NOTCH1 Mature B CD5 CD19 CD20 CD21 CD22 CD23 act CD24 CD27 act CD32 CD35 CD37 CD40 CD48 act CD49b CD49c CD49d CD52 CD72 CD75 CD79a CD79b CD80 act CD84 CD86 act CD98 CD124 CD137 (41BB) act CD152 (CTLA-4) act CD180 CD184 (CXCR4) CD185 (CXCR5) CD192 (CCR2) act CD196 (CCR6) CD200 CD210 CD229 CD252 act CD253 act CD267 (TACI) CD268 (BAFFR) CD269 (BCMA) CD272 CD275 CD279 (PD-1) act CD307 CD317 (BST2) CD319 (CRACC) CD360 (IL-21R) HLA-DR IgD IgG IgM Blimp-1 Bcl-6 Pax5 Plasma Common Myeloid Progenitor (CMP) CD33 CD34 CD45RA CD117 (c-kit) CD123 low CD131 CD164 CD173 CD174 Ikaros PU.1 Megakaryocyte- Erythroid Progenitor (MEP) Granulocyte-Myeloid Progenitor (GMP) CD34 CD45RA CD123 PU.1 CD33 CD34 CD36 CD110 CD123 high CD131 CD164 FOG-1 GATA1 GATA2 Macrophage CD11a CD11c CD14 CD16 CD19 CD23 CD26 CD31 CD32 CD36 CD40 CD45RO CD52 CD63 CD68 CD71 CD74 CD87 CD88 CD92 CD119 CD121b CD123 CD155 CD156a CD163 CD169 CD170 CD195 CD204 CD206 CD210 CD280 CD282 (TLR2) CD284 (TLR4) CD312 CD354 (Trem-1) CD357 (GITR) HLA-DR Trem-2 PU.1 Conventional DC Precursor CD11c CD14 neg CD33 dim Common DC Progenitor CD11c CD33 dim Plasmacytoid DC (pDC) Conventional DC (cDC) Monocyte Mature NK CD2 CD7 CD8 CD11b CD15s CD16 CD27 CD32 CD43 CD45RC CD49d CD56 CD57 CD62L CD66a CD81 CD85d (ILT4) CD85j (ILT2) CD87 CD94 CD96 CD99R CD119 CD122 CD132 CD158a CD158b CD158f CD159a (NKG2A) CD159c (NKG2C) CD160 CD161 CD162R CD178 CD181 (CXCR1) CD182 (CXCR2) CD183 (CXCR3) CD198 CD210 CD212 CD218a CD218b CD223 CD226 CD244 CD245 CD247 CD253 CD277 CD300a CD305 CD319 (CRACC) CD328 (Siglec-7) CD335 (NKp46) CD336 CD337 Granzyme A Granzyme B Perforin Neutrophil Eosinophil Treg CD25 CD39 CD73 CD101 CD127 low CD152 (CTLA-4) CD183 (CXCR3) CD194 (CCR4) CD199 (CCR9) CD357 (GITR) GARP act Foxp3 Helios Th17 CD161 CD121a (IL-1R1) CD196 (CCR6) CD360 (IL-21R) IL-23R BATF IRF4 RORγt Th1 CD26 CD94 CD178 (FASL) CD195 CD212 (IL-12RB2) CD218 (IL-18R) CD254 (TRANCE) CD278 (ICOS) NOTCH3 Tim-3 T-bet Th2 CD30 CD124 (IL-4R) CD184 (CXCR4) CD198 (CCR8) IL-17RB NOTCH1 NOTCH2 Tim-1 GATA3 Th9 CD124 (IL-4R) IL-17RB IRF4 Th22 CD140 (PDGFR) CCR10 Tfh CD84 CD126 (IL-6R) CD185 (CXCR5) CD278 (ICOS) CD360 (IL-21R) Bcl-6 Mast Basophil CD9 CD11a CD11b CD13 CD16 CD17 CD25 CD32 CD33 CD38 CD43 CD88 CD123 CD125 CD218 (IL-18R) CD154 CD192 CD193 CD203c CD281 (TLR1) CD282 (TLR2) CD284 (TLR4) CD286 (TLR6) CD289 (TLR9) CD294 FcεR1 IgE C/EBPα GATA2 Megakaryocyte CD41 CD42a CD42b CD42c CD42d CD49f CD51 CD61 CD110 CD112 CD123 Platelet CD9 CD17 CD23 CD31 CD36 CD41 CD42a CD42b CD42c CD42d CD49b CD49f CD51 CD60a CD61 CD84 CD92 CD110 CD147 CD151 CD173 CD226 GARP Activated Platelets CD54 CD62p CD63 CD107a CD107b CD109 Erythrocyte CD35 CD44 CD123 CD173 CD233 CD234 CD235a CD235b CD236 CD236R CD238 CD239 CD240 CD241 CD242 General T Cell Markers CD2 CD3 CD5 CD6 CD7 CD27 CD28 CD30 CD35 CD40 CD43 CD45RA CD45RB CD45RC CD52 CD57 CD60a CD60b CD60c CD62L CD75s CD81 CD84 CD85j (ILT2) CD89 CD94 CD98 CD99R CD101 CD103 CD107a act CD107b act CD119 CD121b CD122 CD123 CD127 CD130 CD132 CD150 CD158a CD158b CD158f CD159a CD160 CD161 CD168 CD171 CD172a (SIRPα) CD181 (CXCR1) CD182 (CXCR2) CD184 (CXCR4) CD191 (CCR1) CD192 (CCR2) CD193 (CCR3) CD195 (CCR5) CD196 (CCR6) CD197 (CCR7) CD198 (CCR8) CD199 (CCR9) CD210 CDv218a CD218b CD226 CD229 CD244 CD247 CD277 CD279 CD300a CD300c CD305 CD316 CD317 (BST2) CD319 (CRACC) αβ TCR CD4 low CD9 CD11b CD11c CD13 CD14 CD15 CD17 CD28 CD31 CD32 CD33 CD35 CD36 CD38 CD40 CD45RO CD45RA CD45RB CD49b CD49e CD49f CD63 CD64 CD65s CD68 CD74 CD84 CD85a (ILT5) CD85d (ILT4) CD85h (ILT1) CD85j (ILT2) CD85k (ILT3) CD86 CD87 CD89 CD91 CD92 CD93 CD101 CD111 CD112 CD115 CD116 CD119 CD121b CD123 CD131 CD142 CD147 CD155 CD156a CD157 CD162 CD163 CD168 CD170 CD171 CD172a (SIRPα) CD172b CD180 CD184 (CXCR4) CD191 (CCR1) CD192 (CCR2) CD195 (CCR5) CD198 (CCR8) CD210 (IL-10R) CD213a1 (IL-13Rα 1) CD213a2 (IL-13Rα2) CD226 CD277 CD281 (TLR1) CD282 (TLR2) CD284 (TLR4) CD300a CD300c CD300e (IREM-2) CD300f (IREM-1) CD302 CD305 CD312 CD317 (BST2) CD322 CD329 GITRL PU.1 CD10 CD15 CD16b CD17 CD24 CD32 CD35 CD43 CD66a CD66b CD66d CD89 CD93 CD112 CD114 CD116 CD157 CD177 CD181 (CXCR1) CD281 (TLR1) CD282 (TLR2) CD284 (TLR4) CD286 (TLR6) CD289 (TLR9) MPO Trem-1 CD1c CD1d CD2 CD4 low CD11b CD11c CD13 CD14 neg CD33 CD45RO CD64 CD68 CD80 CD85j (ILT2) CD85k (ILT3) CD86 CD105 CD112 CD123 neg CD141 low CD163 CD172a (SIRPα) CD195 (CCR5) CD197 (CCR7) CD205 CD282 (TLR2) CD283 (TLR3) CD284 (TLR4) CD286 (TLR6) CD300e CLEC-9A HLA-DR CD4 CD11c neg CD45RA CD49d CD85g (ILT7) CD85k (ILT3) CD123 CD282 (TLR2) neg CD284 (TLR4) neg CD289 (TLR9) CD303 (BDCA-2) CD304 (BDCA-4) HLA-DR TLR7 Spi-B CD9 CD11b CD15 CD24 CD32 CD35 CD43 CD64 CD116 CD117 (c-kit) CD123 CD125 CD126 CD181 (CXCR1) CD244 FcεR1 IgE CD9 CD11b CD15 CD24 CD32 CD35 CD64 CD244 CD116 CD123 CD125 CD126 FcεR1 IgE –– = Proposed Pathway act = Expressed On Activated low = Low Expresion high = High Expresion neg = Negative = Intracellular Antigen = Subset = Pan Marker/Common Defining Marker LEGEND LYMPHOID MYELOID CD117 (c-kit) low CD127 CD135 (Flt3) CD164 HLA-DR Aiolos c-myb high C/EBPα neg GATA2 neg GATA3 PU.1 high TdT CD7 CD10 CD24 neg CD34 CD38 CD45RA CD90 low CD133 CD138 CD183 (CXCR3) CD184 (CXCR4) CD269 (BCMA) Aiolos Blimp-1 IRF4 XBP-1 CD19 neg CD20 neg CD27 CD252 CD52 neg CD126 CD38 CD72 neg CD79a CD1a CD2 CD3 CD4 CD5 CD7 CD8α CD8β CD45RO CD127 (IL-7Rα) TCRβ ThPOK TOX Differentiation of the Human Hematopoietic Stem Cell Human hematopoietic development from the hematopoietic stem cell to the end stage cells illustrating markers found at each stage as determined through the 9th HLDA congress. Q312061 Hematopoietic Stem Cells 0912 www.eBioscience.com ©2012 eBioscience, Inc. An Affymetrix Company. All rights reserved. Customers in countries where direct sales are not available may contact their eBioscience distributor listed at www.eBioscience.com/distributors Differentiation of the Human Hematopoietic Stem Cell NORTH AMERICA: CUSTOMER SERVICE 888.999.1371 • [email protected] | TECHNICAL SUPPORT 888.810.6168 • [email protected] EUROPE: CUSTOMER SERVICE +43 1 796 40 40-304 • [email protected] | TECHNICAL SUPPORT +43 1 796 40 40-120 • [email protected]
