Michael F. Romero, PhD CURRICULUM VITAE September 2, 2011 · and tissues are being used to...

Michael F. Romero, PhD CURRICULUM VITAE

September 2, 2011

Personal Data

Hispanic

Born, Atlanta, Georgia, USA

Dept. Physiology & Bio medical Engineering

Mayo Clinic College of Medicine

200 First St SW, Rochester, MN 55905

Email [email protected]

Tel. 507-284-8127

url: http://mayoresearch.mayo.edu/mayo/research/romero_lab/

http://discoverysedge.mayo.edu/de09-1-kid-romero-hco3transporters/

Education

Undergraduate: Thomas More College, Crestview Hills, KY.

1984 BA: Biology

Associate Degree: Chemistry and Mathematics

Graduate: Case Western Reserve University, Cleveland, Ohio

1984-88 Dept. Developmental Genetics & Anatomy

1988-91 Dept. Genetics (official)

1986-91 Dept. Physiology & Biophysics (lab relocation)

1/1992 PhD October 4, 1991 (CWRU granted PhD Jan. 1992)

Research advisor: Ulrich Hopfer, MD, PhD

Thesis Title: Angiotensin II regulation of ion transport in the rabbit proximal tubule

Professional Experience

11/91-2/92 Post Doctoral Fellow Dept. Physiology & Biophysics,

Case Western Reserve Univ, Cleveland, OH

2/92-7/95 Post Doctoral Fellow Dept. Cellular & Molecular Physiology,

Yale Univ., New Haven CT

7/95-9/97 Associate Research Scientist1

Dept. Cellular & Molecular Physiology,

Yale Univ., New Haven CT

9/97- 6/03 Assistant Professor Dept. Physiology & Biophysics,

Case Western Reserve Univ, Cleveland, OH

10/98-6/03 Assistant Professor Dept. Pharmacology, CWRU

7/03 – 8/31/06 Associate Professor Dept. Physiology & Biophysics, CWRU

7/03 – 8/31/06 Associate Professor Dept. Pharmacology, CWRU

7/1/06 Award of Tenure Case Western Reserve Univ, Cleveland, OH

9/1/06 – present Associate Professor /

Sr. Associate Consultant

4/20/07-present Associate Professor /

Sr. Associate Consultant

Dept. Physiology & Biomedical Engineering


Div. Nephrology & Hypertension


1 Associate Research Scientist is a non-tenure track faculty appointment.

mailto:[email protected]

http://mayoresearch.mayo.edu/mayo/research/romero_lab/

http://discoverysedge.mayo.edu/de09-1-kid-romero-hco3transporters/

MF Romero Curriculum vitae - September 2, 2011 2

Personal Statement

My background is renal physiology, electrophysiology and ion / solute transporter biophysics. As a postdoctoral fellow with Walter Boron, I used an expression cloning technique with Xenopus oocytes to clone the first cDNA for a Na+/HCO3

- cotransporter (NBCe1, Slc4a4). We have collaborated with Matthias Hediger and several other investigators

to clone and characterize the transport activities of the SLC solute carriers, emphasizing HCO3-, anion and Na+ transport.

Several years ago with Dr. David Mount (Brigham), we cloned and characterized novel electrogenic Cl-/HCO3-

exchangers of the Slc26 family. Since then, we have used human mutations (or cSNPs) to find how function is altered.

We have taken an evolutionary approach to follow our Slc4 and Slc26 genes through evolutionary time, by cloning and expressing ortholog clones particularly from organisms which adapt to unique environments (e.g., euryhaline teleosts, and Diptera insects). Before leaving Yale, I identified and initially characterized a C. elegans Slc4 cDNA (ceNBC, Genbank#AF004926) as an electroneutral Na+/HCO3

- cotransporter (Romero MF, Boron WF. J Am Soc

Nephrol 9: 11A, 1998). In 1998 my lab cloned the first Na+ driven Cl-/HCO3- exchanger (NDAE1) from Drosophila. We

spent several years using this insect clone to understand mammalian transport. In 2000 I help Fei & Leibach characterize currents and H+ transport by 3 C. elegans H+/oligopeptide transporter (J Biol Chem 275: 9563, 2000). We used NDAE1 overexpression in Sf9 cells for membrane protein purification. Unfortunately, this did not work, but we have maintained an interest in structural biology, through modeling (e.g., N-terminus of NBCe1 – JBC 2007) and protein-protein interactions (CaR/Kir4.1/MuppI – AJP 2007, 2009; Slc26a9 with R-region of CFTR – JBC 2009). Recently we have studied transport function of Slc26a6, Slc26a9 and non-mammalian Slc26a5/prestin. Non-mammalian prestins function as Cl-/nHCO3

- exchangers, Cl-/SO42-- exchangers and Cl-/oxalate2- exchangers, allowing models of kidney stones

(oxalate transport) and malaria (Anopheles SO42- transport) to be developed.

We routinely collaborate with laboratories to physiologically characterize ion/solute transporters in Xenopus oocytes (H+ coupled peptide transport, H+ coupled divalent metal transport, H+ coupled folate transport, etc.). Since we normally use glass microelectrodes to determine function of SLC transporters, we are collaborating with Dr. Tian Cui (Mech. Eng., U MN) to biologically verify fabricated, nanosensors to measure ion and solute concentrations. We have collaborated to physiologically characterize HCO3

- transporters (DB Mount, S Hirose, A Kato, P Linser, JA Dow, M

Koelle) and H+ transporters (MA Hediger, F Leibach, B Mackenzie, ID Goldman) as well as other transporters and channels (ME Maguire, RT Miller, K Choe, SJ Russell, J Lieske, VA Lennon, MP Fautsch). These combined projects and approaches allow us to continually hone our transporter expertise. Our current projects use our electrophysiology expertise to physiologically characterize transporters and channel proteins in the kidney, gut and eye. These approaches and tissues are being used to understand the transport and environment of acidosis, kidney stones, glaucoma, GABA- ergic signaling and cystic fibrosis.

Teaching Experience

1981-84 Speech and Debate, Covington Latin High School, Covington, KY

1985-88 Gross Anatomy teaching, CWRU Medical School, Cleveland, OH

Summer ‘93 high school student summer project: electrophysiology

Summer ‘95 supervision of high school biology teacher (APS teacher fellowship):

molecular biology, Xenopus oocyte expression, high school lab development

Summer ‘95 medical student summer project: molecular biology, Xenopus oocyte expression

Summer ‘96 2 Dutch medical students summer project:

molecular biology, Xenopus oocyte expression, electrophysiology

Summer ‘96 2 undergraduates students summer projects (Minority Opportunity Program):

molecular biology (subcloning, PCR), Xenopus oocyte expression, electrophysiology

Summer ’98 SURP-student, Darin Henry (Edinboro U, PA),: Xenopus oocyte expression,

electrophysiology

Summer 2001 SURP-student, Brenda Smith (U Akron, OH): Xenopus oocyte expression, electrophysiology

Fall 2001 Undergraduate intern, Kristin Bartholomew (Edinboro U, PA); molecular biology, bacterial

protein expression

Summer 02-04 SURP-student, Jen DiPiero (Duke): molecular biology, Xenopus oocyte electrophysiology

Summer 03, 05 2003, high school student, Naina Gupta (Schawnee): Western blotting

2005, undergraduate (U Pennsylvania): molecular biology, Xenopus oocyte electrophysiology

Summer 2007 SURF-student, David Ying (MIT): molecular biology, Xenopus oocyte electrophysiology

Summer 2008 SURF-student, Alyona Haritonova (St. Katherine’s): Xenopus oocyte electrophysiology

Summer 2009 SURF-student, Lauren Brin (St. Katherine’s):

Summer 2010 SURF-student, Anna Czapar (Univ Illonois, BME):

Summer 2010 SURF-student, Shannon Newman (Laurence College, Biology):

Summer 2011 SURF-student, Daniel Bondeson (Univ Wisc., Chemistry)


Graduate School Teaching- CWRU

PHOL-532 Molecular Organization of Cells. 10/98 “How to study cells.” 2 hr lecture, 12 graduate and 1 undergraduate students 10/99 “How to study cells.” 2 hr lecture, 14 graduate students

PHOL-460 (Introduction to Molecular Biology.

11/98 “Xenopus oocyte expression,” 1.5 hr lecture, graduate students. 11/99 “Expression Cloning,” 1.5 hr lecture, graduate students. 11/2/00 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/01 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/7/02 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/03 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (18). (1.5 hr total) 11/04 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (10). (1.5 hr total) 11/05 "Yeast, baculovirus, and oocyte expression; Expression cloning" (1.5 hr total)

PHRM 433 (or Physiology 433) Membrane Transport

1999: 5 graduate students 3/24/99 1 hr lecture + 4/7/99 2 hr discussion (3 hr total)

2000: 4 graduate students 11/14/00 1.5 hr lecture 11/16/00 2 hr discussion (3.5 hr total)

PHRM 435 (Integrative Systems Physiology and Therapeutics, ISPT) Renal Physiology

2001: 15 graduate students 1/17/01 2 hr lecture 1/22/01 2 hr lecture (4 hr total)

2002: 15 graduate students 2/11/02 2 hr lecture

(4 hr total) 2/23/02 2 hr lecture 2003: 2 x 2 hr lectures/discussion as previously (4 hr total) 2004: 2 x 2 hr lectures/discussion as previously (4 hr total) 2005: 2 x 2 hr lectures/discussion as previously 1 x 2 hr original paper discussion (6 hr total)

PHOL 398

1999-2000:

Undergraduate Seminar

co-organizer of Dept Seminar Series.

Weekly

PHOL 468 (Membrane Physiology)

2003, Spring: 2 x 1.5 hr lecture / discussion on “Na+ coupled transporters” (4.5 hr total)

2004, Spring: 5 x 1.5 hr lecture / discussion on “Na+

coupled transporters” (7.5 hr total) Na

+ coupled Cotransporters, other Cotransporters, Exchangers, water transport (thermodynamics & kinetics)

2004, Fall: 5 x 1.5 hr lecture / discussion on “Transporters,” (7.5 hr total) Na

+ coupled Cotransporters, other Cotransporters, Exchangers, Cellular Ion Homeostasis,

water transport (thermodynamics & kinetics) 2005, Fall: 3 x 1.5 hr lecture / discussion on “Transporters,” (4.5 hr total)

Cotransporters, Exchangers, and Regulation of cellular pH

PHOL 476 (Cellular Biophysics)

2005, Spring: 2 x 2 hr lecture / discussion on “Thermodynamics & Cellular compartments” (4 hr total)

PHOL 480 (Organ Systems Physiology)

2002, Fall: Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) Discussion 16 10/21 Introduction; Body Fluid Compartments Discussion 17 10/23 Glomerular Filtration, Tubular Function Discussion 18 10/28 Renal Regulation of Extracellular Fluid Volume

2003: Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) 2005, Spring Renal Physiology (3 x 1.5 hrs.) (4.5 hr total) 2006, Spring Renal Physiology (3 x 1.5 hrs.) + Renal Computer lab (1 x 1 hr)

Pulmonary Acid-Base (1 x 1.5 hrs) (7 hr total)


2003, Feb 26 Expression Cloning 1 hr lecture

Bicarbonate transporter gene family(ies) 1 hr lecture

BCH2021H Molecular aspects of ion transport, Biochemistry at University of Toronto, Toronto, Ontario, Canada

CWRU Dental School Teaching Dental Pharmacology: Renal physiology / pharmacology

(2 hr total)

2002: 60 students (4 hr total) 2/11/02 2 hr lecture 2/23/02 2 hr lecture

CWRU Medical School Teaching Homeostasis I 12/98 - 1/99 1/00

Renal Physiology Renal Physiology

Small groups- 5 x 2 hr meetings "Acid-Base Homeostasis"

2 x 1 hr lectures

(17 hr total)

(18 hr total)

1/01

Renal Physiology

Acid-Base Computer Lab Small groups- 5 x 2 hr meetings "Acid-Base Homeostasis"

1 x 4 hr Lab

3 x 1 hr lectures

3 hr 1/02

Renal Physiology

Acid-Base Computer Lab Small groups- "Acid-Base Homeostasis"

1 x 4 hr Lab 5 x 2 hr meetings 3 x 1 hr lectures

4 hr 10 hr 3 hr

1/03

Renal Physiology

Glomerular filtration Computer Lab Acid-Base Computer Lab Small groups- "Acid-Base Homeostasis"

1 x 2 hr Lab 1 x 3 hr Lab 5 x 2 hr meetings 3 x 1 hr lectures

2 hr 3 hr 10 hr 3 hr

Glomerular filtration Computer Lab Acid-Base Computer Lab Small groups-

1 x 2 hr Lab 1 x 3 hr Lab 5 x 2 hr meetings

2 hr 3 hr 10 hr

(18 hr total) 2/03 GI Physiology Small groups- 2 x 2 hr meetings 4 hr (4 hr total) 2/04 GI Physiology Small groups- 1 x 2 hr meetings 2 hr (2 hr total) 2/05 GI Physiology Small groups- 1 x 2 hr meetings 2 hr (2 hr total) 10/04 Cell Physiology Cystic fibrosis small group 1 x 1 hr meeting 1 hr (1 hr total) 10/05 Cell Physiology Cystic fibrosis small group 1 x 1 hr meeting 1 hr (1 hr total) 11/05 Cardiovascular CV small groups + lab 2x1 + 1x1 5 hr (5 hr total)

Fundamentals of Therupeutics - A (FTA) 1998. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories

1999. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2000. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2001. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 2002. FTA Pharmacology lab 2 x 3 hr cat / ferret laboratories 3/2003 FTA “Drug elimination” 1 x 1 hr lecture (7 hrs total) 3/2004 FTA Pharmacology lab 2 x 3 hr ferret laboratory (6 hrs total)

Learner College of Medicine at CWRU Teaching

Renal Physiology / Cell Biology Small Group 11/30/2005 Acid-base/epithelial small group, (2 hrs total)

Mayo Foundation Rochester- Graduate School

Course Dates Presentation title / topic Class time required

Transport Physiology Tutorial – BME independent study

Winter 2007 cell and epithelial transport physiology

4 hr / wk (48 hr)

BME 8870 (Course director) Systems Physiology I (Cell Physiology)-

Fall 07 - present (annual course)

principles cellular physiology 3 hrs /wk (36 hr)

MDPD 5150 10/08-present F-grants- Experimental Design 6 hrs total

MMS 3rd - Basic Science Didactic Block/Lecture Series

Winter 2008 Renal Pathophysiology in Sepsis – Fluid Volume”

1.5 hr

BME 8350: Adv. Topics Molec Biophysics 3/08 - present Biophysics of transporters (2 + 2) 4 hr

Core 6300: Molec Biophysics 3/09-present Biophysics of transporters 8 hr

BME 8859 1/09-(as needed) Tutorial in Renal Physiology 2 hr / wk (24-30 hr)


3

-

Mayo Foundation Rochester- Physiology & Biomedical Engineering (BME)

Service Dates Presentation title / topic Class time

required BME oral qualifying exam committee 2006-present Oral examinations 6-7 students/ yr

BME seminar series - 2008- present Topics cellular physiology and

biomedical engineering

2 hr/wk

(weekly)

Medical Scientist Training Program

selection committee

2008-2010 Select and interview MD/ PhD

candidates

BME graduate student interview 2007- present Select and interview PhD candidates for

Biomedical Engineering graduate

program

As required

Postdoctoral Trainees

Trainee Period Subject Current Position Caroline R. Sussman, PhD Instructor, Romero Lab Support: -postdoc fellowship (MS & NRSA)

-Wadsworth Foundation's Young

Investigator Award

-ACES faculty development Award

-AHA Scientist Development Grant

4/97-10/97

6/00- 6/06

Molecular biology CNS & epithelial cell lineages, zebrafish CNS and epithelial development

Assist. Prof., Physiology & BME,


Rochester, MN

Nick Tripoulas Ph.D 6/98- 6/00 Xenopus oocyte expression Postdoc fellow, Cell Biology, Cleveland

Clinic Foundation w/ Dr. Paul Fox

Janina Szewczyk, MD 12/98-2/99 Molecular biology Clinical fellow in LA

Jose Marcelino, PhD 3/99 – 6/00 Oocyte expression Postdoc in Genetics, CWRU

-current: Biotech policy, State of FL

Lamara D. Shrode, PhD 6/99- 8/00 1) NDAE1 in Drosophila, transfected cells and mammals 2) Molecular biology

Scientific wirting, ProEd Communications,

Beachwood, OH

Dorothee Günzel, PhD 8/99- 8/00 Oocyte electrophysiology C3 Prof. Berlin, Germany

Jun-ichi Satoh, MD Postdoc in Romero lab Support: AHA postdoctoral

fellowship (7/01-6/30/03)

4/00- 7/1/03 1) Cloning and localization of mammalian Mg

2+ transporters

2) Functional analysis of human NBCe1 disease mutations

Assist. Prof., Dept. Pharmacology, Jichi

Medical School, 3311-1 Yakushiji

Minamikawachi, Tochigi

JAPAN 329-0498

Zara Josephs, PhD 6/02 – 5/04 1) Cloning, localization and functional characterization of Slc4 & Slc26 HCO

- transporters

in Drosophila and mosquitos 2) developmental localization of zebrafish Slc4 and Slc26 mRNAs

Postdoc in Romero lab

**Young Investigator award for

Comp. Physiol. Section APS, EB 2003

Fellow, Intl Cancer Res. Institute,

London, UK; 6/04- present

Min-Hwang Chang, PhD Support: AHA postdoctoral fellow

(7/1/04-6/30/06)

Natl.AHA-Scientist Dev. Grant

(1/1/10-12/31/13)

Mayo-UMn Partnership grant

(1/1/10-12/31/11)

CWRU fellow

(8/02 – 8/06) Mayo-Ass. Prof

(8/07-present)

1) Structure-function & mutational analysis of NBCe1, the electrogenic Na

+/HCO3

cotransporter 2) Electrophysiology of Slc26a9 3) Characterization of electrogenic Na

+/

monocarboxylate cotransport by Slc5a8

Postdoc in Romero lab (8/02-9/07)

**Young Investigator award for

Cell & Molec. Physiol. Section APS

EB 2003

Assist. Prof., Physiology & Biomed. Eng.


Rochester, MN

Consuelo Plata, PhD **Mexico Independent Grant

(J48709-III)

“Caracterización funcional y

estructural de los transportadores de

sodio monocarboxilatos

(Na+/MCs;TSMC): electroneutro

(TSMCn/Slc5a12) y electrogénico

(TSMCe/ Slc5a8)”

6/03 – 7/31/06 1) Cloning, localization and electrophysiology characterization of zebrafish & mammalian electrogenic & electroneutral Na

+/

monocarboxylate cotransporters

2) Ion transport by Slc26a9

Departamento de Nefrología y Metabolismo

Mineral, Instituto Nacional de Ciencias

Médicas y Nutrición Salvador Zubirán,.

Vasco de Quiroga #15 Col. Sección XVI,

Tlalpan, D.F., México C.P. 14000

-Postdoc in Romero Lab

**Young Investigator award for Cell &

Molec. Physiol. Section APS, EB 2006


Aleksandra Sinđić Ph.D., M.D. Support: Cystic fibrosis foundation

postdoctoral fellow (5/06-4/08)

Wasantha K. Ranatunga, PhD

1/05 – 8/31/07 1) Immunolocalization of Slc26a9 and Slc5a8 2) Functional interaction of Slc26a9 and CFTR

7/07- present 1) STAS - expression &

purification 2) SLC crystalization

Postdoc in Romero Lab

9/1/07

Assist. Prof., Dept. Physiology

Univ. Zagreb

Zagreb, Croatia


AnPng Chen, PhD 9/07 – present 1)Elasmobranch SLC transporters 2)SLC26A9 – SNP function


Taku Hirata, PhD DK083007-z supplement

11/09 – present 1) Slc26 oxalate transport 2) Drosophila model of stones 3) Slc26 structure


Merfake H. Semret, MD 11/09- 11/10 Nephrology & Hypertension fellow

Doctoral Trainees

Trainee Period Role / Subject Current Position

Christopher M. Sciortino 12/97-4/30/01 Thesis Advisor:

-electrophysiology, molec biology

”Characterization & localization of

the sodium mediated bicarbonate

transporters NBC & NDAE1”

PhD, CWRU, 2001

MD, CWRU – 2005

Surgery Residency

Johns Hopkins, 2005 - present

Theresa Fagan 5/99-7/99 Rotation supervisor:

Oocyte electrophysiology CWRU, Ph.D. 2003- Cell Physiology

(A. Scarpa / A. Romani)

Jane Kim 6/04-7/15/04 Rotation supervisor:

Oocyte electrophysiology PhD program – Cell Physiology

Andreas Fulterer 12/09 – 5/2010 Visiting PhD student:

Transporter biophysics

Oocyte electrophysiology

-microelectrodes

-patch clamping

Universität Stuttgart, Stuttgart, Germany

Yusuke Ito 12/10-4/11 Visiting PhD student:

Transporter biophysics

Oocyte electrophysiology

-microelectrodes

-patch clamping

Biological Sciences, Tokyo Tech, Japan

(S. Hirose lab)

Thesis Committees

Trainee Period Role / Subject Current Position Christopher M. Sciortino, PhD

12/97-4/30/01 Thesis Advisor

-electrophysiology

-molecular biology

PhD Biophysics, 2001; MD, CWRU 2005 Surgery Residency

Johns Hopkins, 2005 - present Joanne Nagy, PhD 6/98-9/14/01 Thesis Committee member Ph.D. - Cell Physiology, (C. Sanders) Kirill Oxenoid, Ph.D. 3/99-12/26/02 Thesis Committee Chair Ph.D. – Biophysics (C. Sanders) Daw-Yang (Darwin) Hwang, MD, PhD

4/99-6/02 Thesis Committee Chair Ph.D. - Cell Physioilogy (F. Ismail-Beigi) -Cardiology Fellow – Tiawain

Lalitha Gudipaty, PhD 5/99-5/03 Thesis Committee member Ph.D.-Cell Physiology (G.Dubyak) Medical student – CWRU

John W. Vince, PhD 2000 External Reviewer: Univ. Toronoto,

Dept. Biochemistry

Ph.D.– Biochemistry, (R.A.F. Reithmeier) "Interaction of Chloride/Bicarbonate Anion Exchangers with Carbonic Anhydrase II"

Brian Wisnoskey, PhD 2001- 2004 Thesis Committee member Ph.D. - Cell Physiology (W. Schilling) Joydeep Sarkar 2002 – present Thesis Committee member Ph.D. candidate–Biomedical Eng, CWRU

(P. Fox – Clev. Clinic Found.) Steve Radabaugh, MS 2003- 7/04 Thesis Committee member M.S. - Cell Physiology (C. Smith) Nichol Baitz, MS 2003-5/05 Thesis Committee member M.S. - Cell Physiology (A. Romani) Suzanne McAlear 2003- 1/07 External Committee Member: Ph.D. candidate @ U AL, Birmingham

Physiology & Biophysics


(M.O. Bevensee-advisor) Shiyuan Ryan Hong 2004-present Thesis Committee member Ph.D. candidate - Cell Physiology

(G. Dubyak) Rebecca Falin 2004-present

2006 defense

Thesis Committee member

Ph.D. candidate - Cell Physiology

(C. Cotton)

Vanderbilt Univ (K. Strange)

Aaron Brister 2005-10/2006 Thesis Committee member

Left program

Ph.D. candidate - Cell Physiology (C. Cotton)

Lizette Warner 11/2006-12/08 Thesis Committee member PhD candidate – Mayo BME (J.C. Romero)

Peter Conrad 1/09-present Thesis Committee member MSTP candidate – Mayo BME (S. Sine)

Carolina Amador Carrascal 11/08-present Thesis Committee member PhD candidate – Mayo BME (J. Greenleaf)

John R. Giudicessi 11/09-present Thesis Committee member MSTP candidate – Mayo Biochem Molec Biol. (M. Ackerman)

Undergraduate / Other trainees

Trainee Period Subject Current Position

Dustin M. Paz 6/96-8/96 molecular biology (subcloning, PCR),

Xenopus oocyte expression,

electrophysiology

Susan Elsby 6/96-8/96 molecular biology, Xenopus oocyte

electrophysiology

MD- Harvard Univ

Resident-surgery UCSF

Unknown

Darin Henry (Edinboro U) 6/98-8/98 Electrophysiology Pharmacia Corporation, CT

Brenda L. Smith (U Akron) 6/01-9/01 Electrophysiology Applying for Medical Technolgy

Kristin Bartholomew

(Edinboro U)

9/01-12/31/01 Molecular biology Unknown

Jennifer DiPiero (Duke) 5/02-8/02;

6/03 - 8/15/03

6/04 – 8/04

-Molecular biology / electrophys.

-zebrafish molecular biology

-

BA-Duke Univ. (5/04)

-Duke Genetics lab (9/04-7/05)

-OH State Dental School (8/05 – 5/09)

-Dental practice OH

Naina Gupta (Schawnee

High School, Lima, OH)

(U. Penn)

Mayo Clinic

6/03 – 8/15/03

6/05 – 8/05

-Molecular biology, immunohisto.

-Molecular biology, electrophys. High school student

U. Penn – Biology Major

Medical School

David Ying (MIT) Summer 07 -Molec. biology (Slc26a9), electrophys. Washington Univ. School of Medicine

(MIT – BME major, 2009)

Alyona Horitonova (St.

Katherine’s, St. Paul, MN)

Summer ‘08 Slc26a2 8/09:Univ MN, Biomedical Eng. (PhD)

(St. Katherine – Physics major, 2009)

Lauren Brin (St. Katherine’s,

St. Paul, MN)

Summer ‘09 -molecular biology (insect Slc26 clones) -electrophysiology

St. Katherine – Chemistry major

8/10-present: U Nebraska Med Sch

Anna Czapar (soph) Summer ‘10 -molecular biology (insect Slc26 clones) -electrophysiology -silkworm protein expression

Shannon Newman (jr) Summer ‘10 -molecular biology -electrophysiology -protein purification and crystalization

Dan Bondeson (jr) Summer ‘11 -Drosophila genetics -electrophysiology (VC & [Cl-]) - tubule YFP fluorescsence

Univ Illinois (Biomedical Engineering)

-applying to MSTP programs

Laurence Univ (Biology)

-8/11: U Wash (Biochemistry)

U WI- Stevens Point (Chemistry)

-applying to grad school


Colleagues / Sabbaticals

Trainee Period Subject Current Position

Daniel Markovich, PhD

Sabbatical

9/02-1/03 Xenopus oocyte electrophysiology of

Slc26 transporters

Associate Professor, Physiology

Univ. Queensland, Brisbane, Australia

**Human Frontiers Science Program, short-term

fellowship for sabadical

Mayo Clinic

Akira Kato, PhD

Sabbatical 7/15-11/07

3/15-4/15/08

10/10–12/10

Xenopus oocyte electrophysiology

Slc26a6 transport

Fugu NHE3 / MCT transport 2+

Assistant Professor, Biological Sciences

Tokyo Institute of Technology,

4259 Nagatsuta-cho, Midori-ku

8/11-10/11 Fugu Mg transport Yokohama-shi, Kanagawa-ken, Fugu borate transport 226-8501 JAPAN

Awards / Honors

1980 Thomas More College, 4 year scholarship St. Xavier University, partial 4 year scholarship

1982 Health Careers Opportunity Program Participant: Univ. Kentucky, Lexington, KY

1983 Summer Research Fellowship: Dept. Biochemistry, Univ. Louisville, Louisville, KY (Research supervisor: Robert Grey, PhD)

1984-86 Graduate Fellowship: Dept. Developmental Genetics & Anatomy, Case Western Reserve University

1986-89 NRSA predoctoral fellowship #HL-07415-08

1991 Travel Award for 1991 ASCB Annual Meeting in Boston, American Society for Cell Biology Minority Affairs Committee

1992 Postdoctoral fellowship (training grant) #DK-07259-15

7/93-7/95 National Kidney Foundation Research Fellowship

1994 a) Am. Physiol. Soc. NIDDK Travel Award, FASEB

b) Am. Physiol. Soc., Gastrointestinal Physiology: Postdoctoral Research Award, FASEB

c) Am. Physiol. Soc., Renal Physiology: Postdoctoral Research Award, FASEB

8/95-8/98 NRSA postdoctoral fellowship #1-F32-DK09342-01

1996 a) Caroline tum Suden/Frances A. Hellebrandt Professional Opportunity Award (APS)

b) Accommodation Award, Society of General Physiologists Annual Meeting

1997 ISN. Travel Grant , XIVth

International Congress of Nephrology, Sidney, May 25-29, 1997

1998 CWRU nominee for Pew Scholar Award

2000 Am. Physiol. Soc., Renal Section: New Investigator Travel Award

2001 a) Cover art- Am. J. Physiol. Cell Physiol. July – Dec 2001

b) Mary Jo Cosio, RN Research Award - National Kidney Foundation of Ohio

2002 APS Careers in Physiology brochure – one of five physiologists featured

2003 APS New Investigator- Cell & Molecular Physiology Section

2006 Tenure, Case Western Reserve University School of Medicine


3

3

-

-

Support: (Completed)

8/1/95-7/31/98 F32-DK009342 (PI:Romero MF) [priority: 146; 19 %-ile]

Expression cloning of Na/HCO3 cotransporter

1/1/97-12/31/00 Am. Heart Assoc. Scientist Development Grant (# 9630322N) (PI: Romero, 30%)

"Physiology, Localization, and Regulation of the renal Sodium/Bicarbonate Cotransporter, NBC"

[total direct = $260,000]

1/1/98-12/31/98 Ireland Cancer Center: CWRU and University Hospitals (ACS-IRG-91-022-05-IRG)

Role of Na/HCO3 Cotransporters, NBC, in Prostate Cancer

4/1/99-3/31/00 CWRU/UH Cystic Fibrosis Core Center Feasibility Grant (PI: Romero, 5%)

"Role of Na/HCO3 Cotransporters, NBC, in the Pancreatic HCO–

Transport"

7/1/00-6/30/02 National Kidney Foundation of Ohio (PI: Romero)

" Cloning and expression of a mammalian Mg2+

transporter " [total direct: $25,000)

7/01/02-6/30/04 NIH-R21-DK60845 (PI: MF Romero) 10%

"HCO–

transporters in Drosophila and mosquitoes" [total direct: $200,000]

5/1/00-4/30/06 NIH-R01-DK56218 (PI: Romero, 50%) [priority: 145; 5.7 %-ile]

"Characterization of the Na+

driven anion exchanger, NDAE1" [total direct: $986,400]

12/01/01-11/30/06 NIH / NIGMS R01-GM61748 (PI: ME Maguire) Romero, 5%

“NRAMPs, Manganese and Salmonella Virulence” [total direct: $750,000)

4/1/06 – 3/31/08 Cystic Fibrosis Foundation, Research Grant (PI: Romero, ROMERO06G0)

"Slc26a9, a novel electrogenic, Na+

transporting Cl/HCO3 exchanger in lung" [direct = $110,000]

(Active)

9/1/06 – 8/31/11 R01-EY017732 (PI: Romero, 30%) [priority: 163; 17.5 %-ile];

"Molecular mechanisms of the electrogenic Na+

Bicarbonate Cotransporter (NBCe1/Slc4a4)"

[total direct = $1,125,000]

7/01/08-6/30/13 R01-CA100634-06 (PI: SJ Russell; Romero 2%); “Radiovirotherapy for multiple myeloma”

9/1/08-8/31/13 P50-DK083007 (PI: Lieske; Admin Core, Romero, 5%)

Mayo Clinic Urology O’Brien Center Research

8/09-7/13 NIH-P30DK084567 [PI: N LaRusso] (Romero MF, associated investigator)

Mayo Clinic Center for Cell Signaling in Gastroenterology

1/1/10-12/31/11 Mayo-UMn Partnership; (PIs: M-H Chang / MF Romero, Mayo; T Cui, UMn)

“Low cost and high performance nano-ionsensors”

9/30/10-9/29/15 P30-DK090728 - Mayo Translational PKD Center (PI: VE Torres)

Model Systems Core B (PI: Romero, 10%)

10/4/10-9/30/11 Mini-sabbadical supplement from NIH-P30DK084567 [PI: Romero MF]

Drosophila model of oxalate stones – proteomics & knockdowns

12/1/10-11/30/12 P50-DK083007-Training Supplement (Romero / Lieske co-PI’s)

Summer Research Fellowships in Urology Research

12/1/11 – 11/30/16 R01-EY021727-01 (MPI) (Fautsch MP, Opthamol / Romero MF – Physiol & BME)

Intraocular pressure regulation via ATP-sensitive potassium channels

(Pending)

(submitted but not funded)

7/1/09-6/30/11 AHA-Grant in Aid: OXALATE transport, binding and Slc26 structure

3/1/09-2/28/10 Bill and Melinda Gates Foundation (PI: Romero)

10/1/10-9/30/14 1R01GM095594-01 (MPI) (PI: Romero MF/ Thompson JR)

“Novel low-cost expression of functional membrane proteins in silkworms”

1/1/11-12/31/14 R01-NS066040-01A2 (MPI) (Koelle MR, Yale / Romero MF, Mayo)

"Chloride transporters that regulate inhibitory neurotransmission"

2/1/11-1/31/13 Oxalosis and Hyperoxaluria Foundation [Romero MF-PI]

“Drosophila model of oxalate nephrolithiasis”

4/1/11 – 3/31/16 R01-EY021727 (MPI) (Fautsch MP, Opthamol / Romero MF – Physiol & BME)

Intraocular pressure regulation via ATP-sensitive potassium channels

7/1/11-6/30/16 R01 DK092408-01 (MPI) [Romero MF, Mayo / Dow JAT, U Glasgow]

“Drosophila model of oxalate nephrolithiasis”

9/1/11-8/31/16 Competitive renewal of R01-DK56218-06A1 (PI: Romero, 30%)

"Slc26a9, a novel electrogenic, Na+ transporting Cl/HCO3 exchanger "


Training Grants – Trainer (Previous @ Case)

9/91 – 8/06 NIH T32-DK007678 (PI: U. Hopfer); Cell Physiology Training Program

7/94 – 5/09 NIH (T32-HL007415) (PI: P.B. Davis); Cystic Fibrosis Research Training Program

7/01 – 6/06 NIH (T32-GM008803) (PI: P.N. MacDonald);

Predoctoral Training Program in Molecular Therapeutics Training Grants – Trainer (Mayo Clinic): (Active)

7/75-11/07 NIH T32DK007013 (PI: VE Torres); - Kidney Disease Research Training Program

7/03-6/13 NIH T32GM065841 (PI: G. Isaya); Medical Scientist Training Program at Mayo Clinic

Mentoring Grants for Trainees: (Previous)

7/1/01 – 6/30/03 American Heart Association- Ohio Valley affiliate PI: Dr. Jun-ichi Satoh

10/1/02 – 12/31/02 Human Frontiers Science Program- short-term fellowship PI: Dr. Daniel Markovich

7/1/04 – 6/30/06 American Heart Association- Ohio Valley affiliate PI: Dr. Min-Hwang Chang

5/1/06 – 4/30/08 Cystic Fibrosis Foundation, postdoctoral fellowship PI: Dr. Aleksandra Sinđić (SINDIC06F0)

(Current / Funded)

1/1/10-12/31/13 AHA-SDG, National PI: Min-Hwang Chang)

1/1/10-12/31/11 Mayo-UMn Partnership; (PIs: M-H Chang / MF Romero, Mayo; T Cui, UMn)

“Low cost and high performance nano-ionsensors”

1/1/10-11/30/10 P50- DK083007z (P50 PI: Lieske; Pilot PI: Taku Hirata);

“Analysis of kidney stone disease using Drosophila genetic model”

Professional Activities:

Memberships:

1984-1993 American Chemical Society

1991-present American Society for Cell Biology

1992-present American Association for the Advancement of Science

1996-present American Physiological Society

1998- present American Society of Nephrology

2007-present American Society of Biochemistry and Molecular Biology (ASBMB)

Editorial Boards:

2000-2003 Am. J. Physiol. - Gastrointestinal & Liver Physiol.

2001-present Am. J. Physiol. Renal Physiol

2004 Advances in Physiology Education – guest Editor for “Cellular Homeostasis”

7/1/07-6/30/12 J. Biological Chemistry

Professional Service:

1997- present ad hoc reviewer

American Journal Physiology (AJP): Renal; AJP: Cell; AJP: GI; AJP: Heart, AJP: Comp & Reg

Physiol,Cancer Research; Current Eye Research; Archives of Biochemistry and Biophysics;

Molecular Endocrinology; Mammalian Genome,; J. Biol. Chem.; PLoS-Biology; Proc. Natl. Acad.

Sci, USA. , J. Am. Soc. Nephrol., Nature, J Cell Physiol., J Gen Physiol., J Physiol. J. Membr.

Biol., FEBS Letters, Insect Molecular Biology, Human Mutation, Human Molecular Genetics

July 1998 MiniSymposium and poster session organiser for Membrane Transport Proteins-Gordon

Conference, Tilton, NH, July 19-24, 1998

1/1/00-12/31/02 American Physiology Society-Education Committee (elected by APS Council)

2000 VA grant review, National Science Foundation ad hoc reviewer

2000-2004 APS-Renal Section abstract reviewer

2000 Am. Soc. Nephrol- abstract reviewer: Cell and Transport Physiology-Acid-Base

2001 Experimental Biology 2001 Symposium Organizer / Chair – APS-Renal section & Epithelia group

"Molecular mechanisms of bicarbonate transport."

2002-2004 Cell Physiology Study Section 4, American Heart Association, Ohio Valley / Southern Affiliate

*2002 NIH ad hoc reviewer, General Medicine B (GMB) Study Section (Oct 2002)

*2003 NIH ad hoc reviewer, Molecular, Cellular, & Developmental Neuroscience Study Section

(MDCN4) Study Section (Jan 2003)

2003 Experimental Biology 2003 Feature Topic Organizer / Chair- APS-Cell section:


"Molecular physiology of bicarbonate transporters."

5/2003 APS rep and judge for Intel International Science and Engineering Fair, Cleveland, OH

*2002-2005 International Union of Physiology Sciences (IUPS) Renal section advisory board for

2005 IUPS meeting scientific program:

*2004 Experimental Biology 2004 Teaching Symposium Organizer / Chair- APS-Education Comm.:

"Cellular homeostasis."

1/1/04-12/31/06 American Physiology Society-Communications Committee (elected by APS Council)

1/1/04 -12/31/06 American Physiology Society-Epithelial Transport Group steering committee (elected)

2005 IUPS / Exp. Biol. 2005 Feature Topic (#37) organizer / Chair: “Acid-base transporters”

*2005-2009 International Union of Physiology Sciences (IUPS) Renal section advisory board for

2009 IUPS meeting scientific program (Kyoto, Japan)

2006-2009 Vice Chair, APS – Cell and Molecular Physiology section (elected)

7/2006-6/2010 NIH study section member, Cellular & Molecular Biology of the Kidney (CMBK)

2006 NSF-ad hoc reviewer ; Deutsche Forschungsgemeinschaft – ad hoc reviewer

2007 BioMedical Transporters 2007 conference (Berne, Switzerland) - “Structure of transporters” Chair / Organizer;

“Plasma membrane transporters: importance in diseases, drug development and drug toxicity” – Co-Chair

2009-2012 Vice Chair, APS – Cell and Molecular Physiology section (elected, 2nd

term)

7/2010 NIDDK Special Emphysis P01 panel

12/2010 NIDDK (ZDK1 GRB-G J2 1) – Training grants + K-grants

CWRU - Professional Service:

4/4/03 Judge for “ShowCase” at CWRU

2004- present Steering Committee, Post Baccalaureate Research Education Program (PREP)

School of Medicine - Professional Service:

1997- present BSTP Admissions Committee: interviewing of graduate student candidates

1998 – present SOM Year 1 teaching – Homeostasis

1998 – 2004 SOM Year 1 teaching – Fundamentals of Therupeutics

2000 Research America representative from Physiology & Biophysics

5/05-5/07 Physiology & Biophysics representative to Case School of Medicine Faculty Council

1/06 – 8/31/06 Member, Vision Science Research Center (http://www.caseeyes.com/index2.html)

Physiology & Biophysics - Professional Service:

1997- present Dept. Admissions Committee: interviewing of graduate student candidates

1998-99 Dept. Seminar Series Co-ordinator

1998 – present graduate student teaching- see Teaching

1998-present Graduate student thesis committees

2002 - 2005 Organizer of Dept. Postdoctoral Recruitment & Mentoring

2003-2005 DPB-web page committee

Pharmacology - Professional Service:

1997- present Dept. Admissions Committee: interviewing of graduate student candidates

1998 – present graduate student teaching- see Teaching

1998-present Graduate student preliminary exam committees

Mayo - Professional Service:

2006-present BME prelim exam committee

2008-present MD-PhD admissions committee

2009-present Research Space, Equipment and Capital Allocation Subcommittee Patents / PCT applications:

5/20/97 filed PCT International application, 44574-5020-WO (amended 5/20/98, currently pending)

NBC, a gene that encodes a member of the bicarbonate transporter family of proteins

2/18/00 filed PCT (pending) "NDAE1, a new bicarbonate transporter."

2/28/03 filed PCT (pending) “Cloning and characterization of SLC26A7 and SLC26A9 anion exchangers”

124/50/3 PCT/US (with Vanderbilt U. and Brigham & Women’s Hospital).

http://www.caseeyes.com/index2.html)


3

3

3

3

-

3

Publications: (Papers)

1. Douglas, JG, M Romero, and U Hopfer. Signaling mechanisms coupled to the angiotensin receptor of proximal tubule

epithelium. Kidney Int 38(Supp. 30): S43-S47, 1990. [3 citations]

2. . [33 citations]

3. Romero, MF, JG Douglas, RL Eckert, U Hopfer, and JW Jacobberger. Development and characterization of rabbit

proximal tubular epithelial cell lines. Kidney Int 42: 1130-1144, 1992. [34 citations]

4. Andreatta-van Leyen, S, MF Romero, MC Khosla, and JG Douglas. Modulation of phospholipase A2 activity and sodium

transport by angiotensin (1-7). Kidney Int 44: 932-936, 1993. [66 citations]

5. Gorodeski, GI, MF Romero, U Hopfer, E Rorke, WH Utian, and RL Eckert. Human uterine cervical epithelial cells

grown on permeable support:a new model for the study of differentiation. Differentiation 56: 107-18, 1994. [52 citations]

6. Gorodeski, GI, U Hopfer, RL Eckert, WH Utian, BJ De Santis, EA Rorke, and MF Romero. Extracellular ATP decreases

accutely and reversibly transepithelial transport through the paracellular pathway in human uterine cervical cells. Am J

Phsyiol 266: C1692-C1698, 1994. [12 citations]

7. Fei, Y-J, Y Kanai, S Nussberger, V Ganapathy, FH Leibach, MF Romero, SK Singh, WF Boron, and MA Hediger.

Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature 368: 563-566, 1994. [600 citations]

8. Gorodeski, GI, D Merlin, BJ De Santis, KA Frieden, U Hopfer, RL Eckert, WH Utian, and MF Romero. Characterization

of paracellular permeability in cultured human cervical epithelia: regulation by extracellular adenosine triphosphate. J

Soc Gynecol Invest 1: 225-233, 1994. [19 citations]

9. Kanai, Y, S Nussberger, MF Romero, WF Boron, SC Hebert, and MA Hediger. Electrogenic properties of the epithelial

and neuronal high affinity glutamate transporter. J Biol Chem 270(28): 16561-8, 1995. [128 citations]

10. Steel, A, S Nussberger, MF Romero, WF Boron, CAR Boyd, and MA Hediger. Stoichiometry and pH-dependence of the

mammalian proton-dependent oligopeptide transporter PepT1. J. Physiol. 498(3): 563-569, 1997. [68 citations]

11. Nussberger, S, A Steel, D Trotti, MF Romero, WF Boron, and MA Hediger. Symmetry of H+

binding to the intra- and

extracellular side of the H+

-oligopeptide cotransporter PepT1. J Biol Chem. 272(12): 7777-7785, 1997. [42 citations]

12. Romero, MF, MA Hediger, EL Boulpaep, and WF Boron. Expression cloning and characterization of a renal electrogenic

Na+/HCO

– cotransporter. Nature 387: 409-413, 1997. [296 citations]

13. Gunshin, H, B Mackenzie, UV Berger, Y Gunshin, MF Romero, WF Boron, S Nussberger, JL Gollan and MA Hediger.

Expression cloning and characterization of a mammalian proton-coupled divalent cation transporter. Nature 388: 482-487,

1997. [1713 citations] PMID- 9242408

14. Nakhoul, NL, BA Davis, MF Romero and WF Boron. Effect of expressing the water channel aquaporin-1 on the CO2

permeability of Xenopus oocytes. Am. J. Physiol. 274:C543-C548, 1998. [174 citations]

15. Romero, MF, P Fong, UV Berger, MA Hediger, and WF Boron. Cloning and functional expression of rNBC, an

electrogenic Na+-HCO– cotransporter from rat kidney. Am. J. Physiol. 274:F425-F432, 1998. [123 citations]

16. Schmitt, BM, D Biemesderfer, EL Boulpaep, MF Romero, and WF Boron. Immunolocalization of the Electrogenic Na+/

HCO–

Cotransporter in Mammalian and Amphibian Kidney. Am. J. Physiol. 276: F27-F36, 1999. [121 citations]

17. Choi I, MF Romero, Khandoudi, N. A Bril, and WF Boron. Cloning and characterization of an electrogenic Na/HCO3

cotransporter isoform from human heart (hhNBC). Am. J. Physiol. 276: C576-584, 1999. [91 citations]

18. Heyer, M., Müller-Berger, S., Romero, M.F., Boron, W.F., Frömter, E. Stoichiometry of rat kidney Na-HCO3

cotransporter expressed in Xenopus laevis oocytes. Pflügers Arch. 438: 322-329, 1999. [39 citations]

19. Sciortino CM and MF Romero. Cation and voltage dependence of the rat kidney, electrogenic Na+/HCO

– cotransporter,

rkNBC, expressed in Xenopus oocytes. Am. J. Physiol., 277: 611-623, 1999. [57 citations]

20. Roussa E, Romero MF, Schmitt BM, Boron WF, Alper SL and Thévenod F. Immunolocalization of AE2 anion exchanger

and Na+-HCO3 cotransporter in rat parotid and submandibular glands. Am. J. Physiol. 277: G1288-96, 1999. [37 citations]

21. Thévenod, F., Roussa, E., Schmitt B. M., and Romero M. F. Cloning and immunolocalization of a rat pancreatic Na+

bicarbonate cotransporter. Biochem. Biophys. Res. Comm. 264: 291-298, 1999. [38 citations]

22. Bevensee, M. O., Schmitt, B. M., Choi, I., Romero, M. F., Boron, W. F. An electrogenic Na/HCO3 cotransporter (NBC)

with a novel C terminus, cloned from rat brain. Am. J. Physiol. Cell Physiol. 278:C1200-1211, 2000. [67 citations]

23. Fei Y-J, MF Romero, M Krause, J-C Liu, W Huang, V Ganapathy, and FH Leibach. A novel H+ symporter, OPT3 from

C. elegans. J. Biol. Chem. 275: 9563-9571, 2000. [18 citations]

24. Grichtchenko, I. I., Romero, M. F., Boron, W. F. Extracellular HCO–

dependence of electrogenic Na/HCO3

cloned from salamander and rat kidney. J. Gen. Physiol 115: 533-546, 2000. [27 citations]

cotransporters

25. Romero MF, D Henry, S Nelson, PJ Harte, AK Dillon and CM Sciortino. Cloning and characterization of a Na+

driven

anion exchanger (NDAE1): a new bicarbonate transporter. J. Biol. Chem. 275: 24552-9, 2000. [66 citations]

26. Sciortino, CM, LD Shrode, BR Fletcher, PJ Harte, MF Romero. Localization of Endogenous and Recombinant Na+-driven

Anion Exchanger Protein, NDAE1, from Drosophila melanogaster. Am. J. Physiol. Cell Physiol. 281: C449-463, 2001.

[15 citations]


27. Ducoudret, O, A Diakov, S Mueller-Berger, MF Romero, and E Frömter. The renal Na-HCO3-cotransporter expressed in

Xenopus laevis oocytes: Inhibition by tenidap and benzamil and effect of temperature on transport rate and stoichiometry.,

Pflügers Arch. 442: (5) 709-717, 2001 [5 citations]

28. Marcelino, J., CM Sciortino, MF Romero, LM Mink, RT Ballock, AN Economides, PM Eimons, RM Harland, ML

Warman. Human disease-causing NOG missense mutations: effects on noggin secretion, dimer formation, and BMP

binding. Proc. Natl. Acad. Sci, USA. 98 (20): 11353-8, 2001. [42 citations]

29. Xie, Qizhi, Rick Welch, Adriana Mercado, Michael F. Romero, and David B. Mount. Cloning of the SLC26A6 anion

exchanger, functional comparison to SLC26A1. Am. J. Physiol. Renal Physiol. 283: F826-838, 2002. [104 citations]

30. Flagg, Thomas P., Dana Yoo, Christopher M. Sciortino, Margaret Tate, Michael F. Romero, Paul A. Welling. Molecular

Mechanism of a COOH-terminal Gating Determinant in the ROMK Channel Revealed by a Bartter’s Disease Mutation J.

Physiol. 544 (2): 351-362, 2002. [13 citations]

31. Hirata Taku, Toyoji Kaneko, Toshihiro Ono, Takeru Nakazato, Norihisa Furukawa, Sanae Hasegawa, Shigeo

Wakabayashi, Munekazu Shigekawa, Min-Hwang Chang, Michael F. Romero, Shigehisa Hirose. Mechanism of acid

adaptation of a fish living in a pH 3.5 lake. Am J Physiol Regul Integr Comp Physiol (January 16, 2003). 284: R1199-

R1212, 2003. [95 citations]

32. Li Hui, Lois Myeroff, Dominic Smiraglia, Michael F. Romero, Theresa Pretlow, Lakshmi Kasturi, James Lutterbaugh,

Graham Casey, Jean-Pierre Issa, Joseph Willis, James K. V. Willson, Christoph Plass, and Sanford Markowitz. SLC5A8:

A novel sodium transporter, is a tumor suppressor gene silenced by methylation in human colonic aberrant crypt foci and

colon cancers. PNAS, USA 100: 8412-8417, 2003. [128 citations]

33. Coady MJ, Chang M-H, Charron FM, Plata C, Wallendorff B, Sah JF, Markowitz SD, Romero MF, and Lapointe J-Y.

The human tumor suppressor gene SLC5A8 expresses a Na/monocarboxylate cotransporter. J Physiol 557: 719-731, 2004

(DOI: 10.1113/jphysiol.2004.063859). [73 citations]

34. Dinour D, M-H Chang, J-I Satoh, BL Smith, A Knecht, I Serban, EJ Holtzman, MF Romero. A Novel Missense Mutation

in the Sodium Bicarbonate Cotransporter (NBCe1/SLC4A4) reveals ion transport defects. J Biol Chem 279: 52238-46,

2004. [73 citations]

35. Choe K, Kato A, Hirose S, Plata C, Sinđić A, Romero MF, Claiborne J, and Evans D. NHE3 in an ancestral vertebrate:

primary sequence, distribution, localization, and function in gills. Am J Physiol: Regulatory, Integrative and Comparative

Physiology 289: R1520-1534, 2005. [38 citations]

36. Mackenzie B, Ujwal ML, Chang M-H, Romero MF, and Hediger MA. Divalent metal-ion transporter DMT1 mediates

both H+-coupled Fe

2+ transport and uncoupled fluxes. Pflügers Arch 451: 544 – 558, 2005. [45 citations]

37. Romero MF, Chang M-H, Plata C, Zandi-Nejad K, Broumand V, Sussman CR, and Mount DB. "Physiology of

electrogenic SLC26 paralogs" In - Epithelial Anion Transport in Health and Disease: the role of the SLC26 transporters

family. Novartis Foundation Symposium 273: 126-147, 2006. [14 citations]

38. Demirci FYK, Chang M-H, Mah TS, Romero MF, and Gorin MB. Proximal renal tubular acidosis and ocular pathology:

L522P, a novel missense mutation in the gene (SLC4A4) for Sodium Bicarbonate Cotransporter protein (NBCe1).

Molecular Vision 12:324-330, 2006. [9 citations]

39. Günzel DM, Kehres DG, Romero MF, and Maguire ME. The MgtC virulence factor of Salmonella enterica serovar

Typhimurium activates Na+, K

+-ATPase. J Bacteriology 188:5586-5594, 2006. [12 citations]

40. Huang C, Sindic A, Chan KW, Hill CE, Hujer KM, Sassen M, Wu Z, Kurachi Y, Nielsen S, Romero MF, and Miller RT.

Interaction of the Ca2+

sensing receptor with the inwardly-rectifying potassium channels inhibits channel function. Am. J.

Physiol 292: F1073-81, 2007 (doi:10.1152/ajprenal.00269.2006). [10 citations]

41. Plata C, Sussman CR, Liang JO, Mount DB, Josephs ZM, Chang M-H, and Romero MF. A novel electroneutral sodium

monocarboxylate transport (SMCTn) is present in zebrafish: A comparison to the electrogenic SMCT (SMCTe/Slc5a8). J

Biol Chem 282 (16): 11996-12009, 2007. [2 citation]

42. Kurita Y, Nakada T, Kato A, Mistry AC, Chang M-H, Romero MF, Hirose S. Identification of intestinal bicarbonate

transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish. Am J

Physiol - Comp & Reg Physiol 284: R1402-1412, 2008. [27 citations]

43. Chang M-H, DiPiero JM, Sönnichsen FD, Romero MF. Entry to "HCO3 tunnel" revealed by SLC4A4 human mutation

and structural model. J Biol Chem 283: 18402-18410, 2008. [10 citations]

44. Chang M-H*, Plata C*, Zandi-Nejad K, Sinđić A, Sussman CR, Raghuram V, Mount DB, and Romero MF. Slc26A9 -

anion exchanger, channel and Na+

transporter. J Membr Biol 228: 125-40,2009 [11 citations] PMID- 19365592

45. Unal ES, Zhao R, Chang M-H, Fiser A, Romero MF, and Goldman ID. The Impact of Histidine Residues on the

Structure/Function of the Human Proton-Coupled Folate Transporter (HsPCFT), SLC46A1. J Biol Chem 284: 17846-

17857, 2009 [15 citations]

46. Sindic A, Chen AP, Huang C, Romero MF, and Miller RT. MUPP1 complexes renal K+

channels to alter surface

expression and transport. Am J Physiol Renal 297: F36-45, 2009.


47. Sussman CR, J Zhao, C Plata, JLC Daly, N Angle, J DiPiero, IA Drummond, JO Liang, WF Boron, MF Romero, MH

Chang. Cloning, localization and functional expression of the electrogenic Na+

bicarbonate cotransporter (NBCe1) from

zebrafish. Am J Physiol - Cell Physiol 297: C865-75, 2009. [5 citation] PMID 19625604.

48. Chang M-H, Plata C, Sinđić A, Ranatunga WK, Chen AP, Zandi-Nejad K, Chan KW, Thompson J, Mount DB, Romero

MF. Slc26a9 is inhibited by the R-region of CFTR via the STAS domain. J Biol. Chem. 284: 28306-28318, 2009. [8

citations] PMID- 19643730

49. Kato A, Kurita Y, Nakada T, Ogoshi M, Nakazato T, Doi H, Chang M-H, Hirose S, and Romero MF. Identification of

renal transporters involved in sulfate excretion in marine teleost fish. Am J Physiol Comp & Reg Physiol: 297: R1647-

1659, 2009 [4 citation] PMID 19812358

50. Mahadeo K, Bove N, Unal E, Teo J, Zhao R, Chang M-H, Fulterer A, Romero MF, and Goldman ID. Properties of the

Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary

folate malabsorption. Am J Physiol - Cell Physiol doi:10.1152/ajpcell.00113.2010, 2010. PMID 20686069

51. Desmoulin SK, Wang Y, Wu J, Stout M, Hou Z, Fulterer A, Chang M-H, Romero MF, Cherian C, Gangjee A, and

Matherly LH. Targeting the proton-coupled folate transporter for selective delivery of 6-substituted pyrrolo[2,3-d]-

pyrimidine antifolate inhibitors of de novo purine biosynthesis in the chemotherapy of solid tumors. Molecular

Pharmacology doi:10.1124/mol.1110.065896, 2010. PMID 20686069

52. Romero MF. SNPs of metabolism, not stones. Am J Physiol Renal Physiol 299: F702-703, 2010, 2010.PMID 20685824

53. Bellemer A, T Hirata, MF Romero, and MR Koelle. Two types of chloride-extruding transporters are required for GABAA

receptor-mediated inhibition in C. elegans. EMBO J 30; 1852 - 1863, 2011. PMID 21427702

54. Roy-Chowdhury U, Bahler CK, Hann CR, Chang M-H, Resch ZT, Romero MF, and Fautsch MP. Activation of

Adenosine Triphosphate-Sensitive Potassium (KATP) Channels Decreases Intraocular Pressure in the Anterior Chamber

of the Eye. Invest Ophthalmol Vis Sci, 2011; DOI: 10.1167/iovs.11-7523 (in press)

55. Islam Z, Kato A, Romero MF, Hirose S. Identification and Apical Membrane Localization of an Electrogenic Na+/Ca

2+

Exchanger NCX2a Involved in Renal Ca2+

Excretion by Seawater Fish. Am J Physiol Regu, 2011 (in press)

doi:10.1152/ajpregu.00165.2011

(Manuscripts submitted / in revision)

1. Hirata T, Cabrero P, Berholtz DJ, Thompson JR, Dow JAT, and Romero MF. In vivo Drosophila model of calcium

oxalate nephrolithiasis. Science (in review), 2011.

2. Hinson SR, Romero MF, Popescu BF, Lucchinetti CF, Fryer JP, Wolburg H, Fallier-Becker P, Noell S, and Lennon VA.

Diverse Pathophysiological Outcomes of IgG Binding to Aquaporin-4 in Living Cell Membranes. PNAS, USA, 2011 (in

revision)

3. Chen A-P, MH Chang and Romero MF. A regulatory loop within STAS domain controls Cl- transport in human

SLC26A9. J Biol Chem, 2011 (in revision)

4. Plata C, M-H Chang, GT Babcock and MF Romero. Cloning and function of a Xenopus Sodium Monocarboxylate

Cotransporter. Am. J. Physiol Cell Physiol, in revision

5. Kimura Y, Kato A, Kurita Y, Chang M-H, Kasai K, Fujiwara T, Hirata T, Doi H, Romero MF, and Hirose S. Borate

excretion by seawater fish uses a renal Slc4a11-borate uniporter. Embo J (in revision), 2011

6. Umezawa T, Kato A, Ogoshi M, Ookata K, Doi H, Romero MF, and Hirose S. O2-filled swimbladder employs

monocarboxylate transporters for the generation of O2 by lactate-induced Root effect hemoglobin. Plos ONE, 2011.

7. Michelle Visentin, Min-Hwang Chang, Michael F. Romero, I. David Goldman. Substrate-specific folate transport

mediated by OATP2B1 at acidic pH. Molec. Pharmacol., 2011 (in revision)

8. Wang L, Desmoulin SK, Cherian C, Polin L, White K, Kushner J, Fulterer A, Chang M-H, Mitchell S, Romero MF, Hou

Z, Matherly LH, and Gangjee A. Synthesis, biological and antitumor activity of a highly potent 6-substituted pyrrolo[2,3-

d]pyrimidine thienoyl antifolate inhibitor with transport selectivity for the proton-coupled folate transporter and folate

receptor over the reduced folate carrier that inhibits β-glycinamide ribonucleotide formyltransferase. J Medicinal

Chemistry, 2011 (submitted).

9. Chang M-H, Plata C, Kurita Y, Kato A, Hirose S, and Romero MF. Functional characterization & physiology of NBCe1

from a euryhaline teleost. Am J Physiol Cell Physiol (in revision), 2011.

10. Chen AP, Chang M-H, and Romero MF. Functional analysis of non-synonymous single nucleotide polymorphisms in

human SLC26A9. Human Mutation: submitted, 8/17/2011, 2011


3

3

3

3

(Manuscripts in preparation)

(Invited Manuscripts & Book Chapters)

1. Liedtke, CM, M Romero, and U Hopfer. 1988. Adrenergic control of the Na, K, 2Cl cotransporter in airway epithelial

cells. in Cellular and Molecular Basis of Cystic Fibrosis, G. Mastella and P.M. Quinton (eds.). San Francisco Press, Inc,

San Francisco, CA, pp. 307-313.

2. Romero, MF, Z Madhun, U Hopfer and JG Douglas. 1990. An Epoxygenase metabolite of arachidonic acid, 5,6-epoxy-

eicosatrienoic acid mediates angiotensin-induced natriuresis in proximal tubular epithelium. in Adv. in Prostaglandin,

Thromboxane, and Leukotriene Research: 7th International Congress on Prostaglandins and Related Compounds, Vol. 21

A (eds. Samuelsson, Paoletti and Ramwell). Raven Press, New York, New York, pp. 205-208. [40 citations]

3. Boron, WF, MA Hediger, EL Boulpaep, and MF Romero. The renal electrogenic Na/HCO3 cotransporter. J Exp. Biol.

200: 263-268, 1997. [38 citations]

4. Boron, WF, P Fong, MA Hediger, EL Boulpaep, MF Romero. The electrogenic Na/HCO3 cotransporter. Weiner

Klinische Wochenschrift, 109: 445-456, 1997. [10 citations]

5. Romero, MF, Y Kanai, H Gunshin and MA Hediger. Expression cloning using Xenopus laevis oocytes.

Meth Enz., 296: 17-52, 1998. [59 citations]

6. Romero, MF and WF Boron. Electrogenic Na/HCO3 cotransporters: Expression cloning and physiology.

Ann. Rev. Physiol. 61: 699-723, 1999. [138 citations]

7. Romero, M. F., C. M. Sciortino, E. Roussa, and F. Thévenod. Na+

/HCO–

cotransporters in various species and organs.

In: 3rd Annual Borelli Conference: Acid-base balance, edited by N. DeSanto. Napoli, Italy:, 1999.

8. Romero, MF. The electrogenic Na+/HCO

– cotransporter, NBC. Journal of the Pancreas (online) 2: 182-191, 2001. [13

citations]

9. Satoh, J-I, and MF Romero. Mg2+

transport in the kidney. Biometals 15: 285-295, 2002. [7 citations]

10. MA Hediger, DM Mount, A Rolfs, MF Romero. The Molecular Basis of Solute Transport. In “The Kidney.” Ed B.M.

Brenner, 7th

edition, 2004, pp.261-308.

11. Romero, MF. Defects of the electrogenic Na+/HCO

– cotransporter, NBC1/SLC4a4. In: Membrane transporter diseases,

edited by Bröer S and Wagner C. New York: Kluwer Academic/Plenum Publishers, 2004, p. 65-79.

12. Hediger MA, Romero MF, Peng J-B, Rolfs A, Takanaga H, and Bruford E. The ABC's of Solute Carriers: Physiological,

pathological and therapeutic implications of human membrane transport proteins: Introduction. Pflügers Arch 447: 465-

468, 2004. [266 citations]

13. Mount DB and Romero MF. The SLC26 gene family of multifunctional anion exchangers. Pflügers Arch 447: 710-721,


14. Romero, MF, CM Fulton, & Boron, WF. The SLC4 gene family of HCO -


transporters. Pflügers Arch, 447:495-509,

15. Romero MF. In the beginning, there was the cell: cellular homeostasis. Adv Physiol Ed 28: 135-8, 2004.

16. Romero MF. Molecular pathophysiology of SLC4 bicarbonate transporters. Curr Opin Nephrol Hypertens 14: 495-501,


17. Mount DB, Kwon CY, Plata C, Romero MF, and Zandi-Nejad K. SLC5 Sodium-Anion Cotransporters and Renal Urate

Transport. In: Renal Stone Disease: Proceedings of the First International Urolithiasis Research Symposium. A.P. Evan,

J.E. Lingeman, and J.C. Williams, eds. American Institute of Physics, Melville, NY, 2007.

18. Sinđić A, Chang MH, Mount DB, Romero MF. Renal physiology of SLC26 anion exchangers. Curr Opin Nephrol

Hypertens 16: 484-490, 2007. [16 citations]

19. Romero MF, Chang M-H and Mount DB. From cloning to structure, function, and regulation of chloride-dependent and

chloride independent bicarbonate transporters. In: Physiology and Pathology of Chloride Transporters and Channels in

the Nervous System: From molecules to diseases, F. Javier Alvarez-Leefmans M, Eric Delpire (ed): Elsevier Academic

Press, 2009, p. 43-80.

20. Dow JAT, Romero MF. Drosophila provides rapid modelling of renal development, function and disease. Am J Physiol

Renal Physiol: 299: F1237-1244, 2010. (doi:10.1152/ajprenal.00521.2010)

21. Kato A and Romero MF. Regulation of Electroneutral NaCl Absorption by the Small Intestine. Annual Review of Physiology vol 73: 261-81, 2011. [2 citations] PMID: 21054167

22. Sindic A, Sussman CR, Romero MF. Primers on Molecular Pathways - Bicarbonate transport by the pancreas. Pancreatology 10:660-4, 2010 (doi: 10.1159/000323435)

(Invited Reviews in preparation)

1. Romero MF and M-H Chang. CSH Protocols. “Electrophysiology using Xenopus oocytes ” 2011.

2. Romero MF, Chang M-H, Plata C, Sinđić A, Sussman CR, Chen AP, Ranatunga WK, Hirata T, Kato A, Hirose S, Mount

DB. Bicarbonate and Na-bicarbonate transporters – role of evolutionary nitches. J Exp. Biol, 2011.


-

(Abstracts)

1. JW Jacobberger, U Hopfer, CM Liedtke, MF Romero, and TL Sladek. Characterization of cystic fibrosis airway

epithelial cells immortalized by transduction of the SV40 large T antigen gene. Society for Analytical Cytology.

Breckenridge, CO. , 1988 abstract.

2. JW Jacobberger, U Hopfer, CM Liedtke, MF Romero, and TL Sladek. Immortalization of airway epithelial cells by

transduction of wild-type and mutant SV40 large T antigen genes. Pediatric Pulmonology 126A. Presented at 1989

North America Cystic Fibrosis Conference, Tarpon Springs, Fla., Oct. 11-14, 1989.

3. MF Romero, JG Douglas, and U Hopfer. 1989. Angiotensin II increases Na+ absorption in early S1 primary cultures. J.

Cell Biol. 109(4):210 (Abstract).

4. MF Romero, JG Douglas, and U Hopfer. 1990. P450 monooxygenase metabolite of arachidonic acid inhibits Na+

absorption in early S1 primary cultures. Kidney Int. 37: 350 (Abstract).[3 citations]

5. S Andreatta-van Leyen, MF Romero, MC Khosla, CM Ferrario, and JG Douglas. 1990. Angiotensin 1-7 (Ang 1-7)

stimulates phospholipase A2 (PLA2) and modulates Na+ Transport in renal tubular epithelium (RTE). Presented at

Council for High Blood Pressure Research 44th Annual Fall Conference (sponsored by Am. Heart Assoc.), Baltimore,

MD, Sept. 12-15, 1990. Hypertension 16(3):328, 1990. [5 citations]

6. MF Romero, JG Douglas, RL Eckert, U Hopfer, and JW Jacobberger. Development and characterization of new proximal

tubular epithelial cell lines. J. Cell Biol. 115: 359, 1991.

7. GI Gorodeski, MF Romero, and WH Utian. Human ectocervical epithelial cultures grown on porous filter: electrical and

morphological characteristics. J. Cell Biol. 115: 358, 1991.

8. MF Romero, JW Jacobberger, and U Hopfer. Angiotensin II and bradykinin stimulated electrogenic ion transport in the

proximal tubule epithelial cell line vEPTa-PL. Biophys. J. 61: A536, 1992.

9. GI Gorodeski, MF Romero, RL Eckert, U Hopfer. Regulation of paracellular transport in human uterine cervical

epithelium. Mol. Biol. Cell 3: 219, 1993. [3 citations]

10. EM Hogan, MF Romero, BA Davis, WF Boron. Shrinkage-induced activation of Na-H exchange in Xenopus oocytes.

FASEB J. 7: A442, 1993.[1 citation]

11. U Hopfer, MF Romero, Z Madhun, JG Douglas. Angiotensin II responses of proximal tubule cells in primary culture and

in a newly generated proximal tubular Cell line. XIIth International Congress of Nephrology. Jerusalem, Israel, June,

1993.

12. MF Romero, Y Kanai, MA Hediger, WF Boron. Acid-base transport is coupled to the electrogenic high-affinity

glutamate transporter, EAAC1. J. Am. Soc. Nephrol. 4:846, 1993.

13. MF Romero, S Nußberger, Y Kanai, MA Hediger, WF Boron. H+ Cotransport is coupled the electrogenic oligopeptide

transporter, PepT1.For presentation at FASEB in Anaheim, CA., FASEB J. 8: A561, 1994.

14. MF Romero, S Nußberger, MA Hediger, and WF Boron. H+ Cotransport Via the electrogenic oligopeptide transporter

PepT1 is bidirectional and Depends on external pH. J. Am. Soc. Nephrol. 5: A260, 1994.

15. MF Romero and WF Boron. Expression of kidney poly(A)-RNA in Xenopus oocytes speeds the CO2 -induced

intracellular acidification. FASEB J. 9: A260, 1995.

16. NL Nakhoul, MF Romero, BA Davis, and WF Boron. Expression of CHIP28 (Aquaporin 1) in Xenopus oocytes

accelerates the CO2 -induced decrease of intracellular pH (pHi). J. Am. Soc. Nephrol. 6: A312, 1995.

17. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Expression cloning of the renal electrogenic Na/HCO3

cotransporter (NBC-1) from Ambystoma tigrinium. FASEB J. 10: A89, 1996.

18. NL Nakhoul, MF Romero, A Waheed, BA Davis, R Mullins, G Hageman, WS Sly, and WF Boron. Processing and

functional expression of carbonic anhydrase isoforms in Xenopus laevis oocytes. FASEB J. 10: A88, 1996.

19. S Nussberger, A Steel, MF Romero, CAR Boyd, WF Boron, and MA Hediger. Proton coupling and charge movement of the

proton-dependent oligopeptide transporter PepT1. FASEB J. 10: A79, 1996.

20. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Expression Cloning of the Renal Electrogenic Na/HCO3

Cotransporter (NBC) from Ambystoma tigrinum. The Physiologist 39: 144, 1996

21. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the Cloned Renal Electrogenic Na/HCO3

Cotransporter, NBC. J. Gen. Physiol. 108: 16-17a, 1996.

22. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the cloned Ambystoma tigrinum Renal Electrogenic

Na/HCO3 Cotransporter (aNBC): I. Role of HCO3 J. Am. Soc. Nephrol. 7: 1259, 1996.

23. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. Physiology of the cloned Ambystoma tigrinum Renal Electrogenic

Na/HCO3 Cotransporter (aNBC): II. Localization and Na+ Dependence. J. Am. Soc. Nephrol. 7: 1260, 1996.

24. MF Romero, MA Hediger, and WF Boron. Cloning and Functional Expression of the Rat Renal Electrogenic Na/HCO3

Cotransporter (rNBC). J. Am. Soc. Nephrol. 7: 1259, 1996.

25. II Grichtchenko, MF Romero, and WF Boron. Extracellular bicarbonate dependence of aNBC, the electrogenic Na/HCO3

cotransporter cloned from tiger salamander (Ambystoma tigrinum ). J. Am. Soc. Nephrol. 7: 1255, 1996.


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3

3

26. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter (rNBC)

Selected for oral presentation in “Latest Advances: Theme I” at the XIV th International Society of Nephrology, May 25, 1997,

Sydney, Australia.

27. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter (rNBC)

FASEB J. 11: A25, 1997.

28. II Grichtchenko, MF Romero, and WF Boron. Extracellular bicarbonate dependence of the cloned rat electrogenic Na/HCO3

cotransporter (rNBC). For presentation at the XXXIII th International Congress of Physiological Sciences, St. Petersburg,

Russia, 1997.

29. BM Schmitt, D Biemesderfer, EL Boulpaep, MF Romero, and WF Boron. Immunolocalization of the electrogenic Na/HCO3

cotransporter (NBC) in mammalian and amphibian kidney. J. Am. Soc. Nephrol. 8:10A , 1997.

30. II Grichtchenko, MF Romero, and WF Boron. Electrogenic Na/HCO3 cotransporters (NBC) from rat and salamander kidney

have similar external HCO–

dependencies. FASEB J. 12: A638, 1998. [1 citation]

31. MO Bevensee, BM Schmitt, MF Romero, and WF Boron. Cloning of a putative Na/HCO3 cotransporter (NBC) from rat

brain. FASEB J. 12: A1031, 1998. [1 citation]

32. BM Schmitt, D Biemesderfer, S Breton D Brown, R Caroppo, JP Geibel, M Ikuma, V Rajendran, EL Boulpaep, MF Romero,

and WF Boron. Immunolocalization of the electrogenic Na+/HCO

– cotransporter (NBC) in mammalian epithelia. Pflüger

Arch. 435: R59, 1998.

33. Rimmer, SJ, Romero, MF, Guggenheim, JG, Wigham, CG, Hodson, SA. Demonstration of a sodium-bicarbonate

cotransporter (NBC) mRNA in rabbit corneal endothelium. Exp. Eye Research 67: S8, 1998.

34. Romero, MF, Sussman, CR, Choi, I, Hediger, MA and Boron, WF. Cloning of an electrogenic Na/HCO3 cotransporter

(NBC) isoform from human kidney and pancreas. J. Am. Soc. Nephrol. 9: 11A, 1998.

35. Romero, MF, Davis, BA, Sussman, CR, Bray-Ward, P, Ward, D and Boron, WF. Identification of multiple genes for human

electrogenic Na/HCO3 cotransporters (NBC) on 4q. J. Am. Soc. Nephrol. 9: 11A, 1998.

36. Romero, MF and Boron, WF. Identification and expression of an electroneutral Na/HCO3 cotransporter from

Caenorhabditis elegans (ceNBC). J. Am. Soc. Nephrol. 9: 11A, 1998.

37. Müller-Berger, S, Heyer, M, Romero, MF , Boron, WF and Frömter, E. Stoichiometry of rat Na+-HCO3 cotransporter

(rkNBC) overexpressed in Xenopus laevis oocytes measured using giant patches. J. Am. Soc. Nephrol. 9: 9A, 1998.

38. Sciortino CM, & Romero MF. Na+

and voltage dependence of the rat kidney electrogenic Na/HCO3 cotransporter (rkNBC)

expressed in Xenopus oocytes. J. Am. Soc. Nephrol. 9: 12A, 1998.

39. M. Heyer, S. Müller-Berger, M.F. Romero, W.F. Boron and E. Frömter. Symmetry test of rat renal Na-HCO3 cotransporter

(rkNBC) overexpressed in Xenopus laevis oocytes. Pflüger Arch. 1999. Presented at the German Physiology Society, Dec,

1998, Bonn, Germany.

40. Thévenod, F. Roussa, E., Schmitt BM, & Romero MF. Molecular cloning, biochemical characterization and

immunolocalization of the rat pancreatic Na+

HCO–. FASEB J. 13: A73, 1999.

41. Choi I, Aalkjaer C, Romero MF, and Boron WF. Cloning and characterization of an electroneutral Na-driven bicarbonate

transporter from rat pulmonary arteries. FASEB J. 13: A400, 1999. [1 citation]

42. Mackenzie B, Ujwal ML, Rolfs A, Berger UV, Romero MF, and Hediger MA. Probing the molecular mechanisms of the H+-

coupled metal-ion transporter DCT1 and the regulation of its expression. FASEB J. 13: A537, 1999.

43. M Ganz, A. K. Dillon, C. M. Sciortino, and M. F. Romero. The Na+ driven Cl-HCO3 exchanger is Actually a Na+ Driven Anion Exchanger, NDAE1. J. Am. Soc. Nephrol. 10: 4A, 1999.

44. Romero, M. F., D. Henry, S. Nelson, E. P. Jane, P. J. Harte, and C. M. Sciortino. Cloning, Localization and Expression of a

Na+ Driven Anion Exchanger, NDAE1. J. Am. Soc. Nephrol. 10: 8A, 1999.

45. Sciortino, C. M., and M. F. Romero. Characterization of Leak Current, Substrates, and Inhibitors of the Na+-driven Anion

Exchanger (NDAE1). J. Am. Soc. Nephrol. 10: 9A, 1999.

46. Romero, M. F., A. K. Dillon, P. J. Harte, and C. M. Sciortino. The Drosophila ndae1 Gene Encodes a Functional Na+

Dependent Cl-HCO3 Exchanger. Presented at NIH Workshop-Advances in Membrane Transport, Bethesda, MD; Dec 6-7,

1999.

47. Sciortino, C. M., and M. F. Romero. Anion and cation transport properties of the Na+

driven anion exchanger (NDAE1)

expressed in Xenopus oocytes. FASEB J. 14: A356, 2000. [1 citation]

48. Romero, M. F., A. K. Dillon, P. J. Harte, and C. M. Sciortino. The Drosophila ndae1 Gene Encodes a Functional Na+

Dependent Cl-HCO3 Exchanger. FASEB J. 14: A356, 2000.

49. Romero, MF, F Thévenod, E Roussa, LD Shrode, & CM Sciortino. Physiology and Localization of Electrogenic Na/HCO3

Cotransporters (NBC). American Heart Association Research Symposium, May 6, 2000, Dallas, TX

50. Moffett, D. F., S.B. Moffett and M.F. Romero. Immunolocalization of a Na+-dependent anion exchanger (NDAE1) in

anterior stomach of mosquito larvae (Aedes aegypti): relevance to gut alkalinization. Society for Insect Cell Biology,

Chicago, Il., Jan. 2001, AM ZOOL 40: (6) 1134-1134 DEC 2000.

51. Sciortino, CM and MF Romero. Functional Characterization of a Na+/HCO

– Cotransporter Point Mutation that results in

Type II Renal Tubular Acidosis. FASEB J 15: (4) A502-A502 Part 1 MAR 7 2001. [2 citations]


3

52. Sciortino, CM, LD Shrode, BR Fletcher, PJ Harte, and MF Romero. NDAE1, Na+–driven anion exchanger from Drosophila,

is a Plasma Membrane Protein Expressed in the Nervous System and Alimentary Tract FASEB J 15: (4) A503-A503, 2001.

[1 citation]

53. Romero, MF, and BL Smith. Mutations in the Na+/HCO

– cotransporter result in biophysical alterations causing renal tubular

acidosis and ocular pathologies. FASEB J 16:A52-3, 2002.

54. Xie, Qizhi2, Rick Welch

2, Adriana Mercado

1,2, Michael F. Romero

3, and David B. Mount

1,2. Cloning of the SLC26A6 anion

exchanger, functional comparison to SLC26A1. FASEB J 16: A807, 2002.

55. Xie, Qizhi2, Rick Welch

2, Luyan Song

,2, Michael F. Romero

3, and David B. Mount

1,2. Cloning of multiple novel members of

the SLC26 gene family of anion exchangers. FASEB J 16: A807, 2002.

56. Xie, Qizhi2, Michael F. Romero

3, David B. Mount

1,2. Multiple novel members of the SLC26 gene family of anion

exchangers. Days of Molecular Medicine 2002 conference, March 13-16, 2002 at The Salk Institute in La Jolla, California.

57. Chang M-H, DiPiero J, Sönnichsen FD, and Romero MF. Mutational analysis predicts structure and ion transport role for the

N-terminus of the electrogenic Na+ bicarbonate cotransporter, NBC1 (SLC4a4). FASEB J 17: A462, 2003.

**Young Investigator award for Cell & Molecular Physiol. Section APS, EB 2003

58. Josephs Z, Satoh J-i, Chang M-H, Mercado A, Zandi-Nejad K, Mount DB, and Romero MF. Cloning and expression of

Drosophila Slc4 and Slc26 homologs. FASEB J 17: A462, 2003.

**Young Investigator award for Comparative Physiol. Section APS, EB 2003

59. Mackenzie B, Ujwal ML, Chang M-H, Romero MF, and Hediger MA. Resolving the mechanisms of the iron transporter

DCT1 from kinetic properties and the impact of mutating two critical histidyl residues within transmembrane regions.

FASEB J 17: A911, 2003. [1 citation]

60. Mercado A, Zandi-Nejad K, Romero MF, and Mount DB. Comparative physiology of Xenopus laevis SLC26 exchangers.

FASEB J 17: A464, 2003.

61. Zandi-Nejad K, Enck A, Mercado A, Romero MF, and Mount DB. The three Xenopus laevis orthologs of mammalian

pendrin/SLC26A4 differ in oxalate transport and DIDS-sensitivity. J Am Soc Nephrol, 14: 542A, 2003.

62. Chang M-H, Satoh J-I, Brenda L. Smith, and Romero MF. The S427L mutation in human kidney NBC1 (SLC4A4) is a

transport defect. Faseb J 18: A1269, 2004.

63. Josephs ZM and Romero MF. Identification & localization of Slc4 & Slc26 anion transporters in Drosophila and zebrafish.

Faseb J 18: A317, 2004.

64. MF Romero, DB Mount*, J-I Satoh, BL Smith, J DiPiero, N Angle, Z Josephs, CR Sussman, C Plata, F Sönnichsen, M-H

Chang. Sodium/bicarbonate cotransporters and new bicarbonate transporters in the kidney. Clinical and Experimental

Nephrology, 2004.

65. Plata C, Josephs ZM, and Romero MF. Cloning, Physiology & Localization of Xenopus and zebrafish Sodium

Monocarboxylate Cotransporters. J Am Soc Nephrol 15: 77A, 2004.

66. Romero MF, Chang M-H, DiPiero JM, Josephs ZM, Sussman CR, Angle NM, and Plata C. Cloning, expression &

localization of a renal electrogenic Na+

bicarbonate cotransporter from zebrafish, zNBCe1. J Am Soc Nephrol 15: 75A,

2004.

67. Zandi-Nejad K, Plata C, Enck AH, Romero MF, Mercado A, and Mount DB. Slc5a8 functions as a sodium-dependent

pyrazinoate and nicotinate cotransporter; implications for renal urate transport. J Am Soc Nephrol 15: 89A, 2004.

68. Chang M-H, Plata C, and Romero MF. Acid-base transport by the tumor suppressor SLC5A8, an electrogenic

Na+/butyrate & Na+/lactate cotransporter. J Am Soc Nephrol 15: 75A, 2004.

69. Chang M-H, Plata C, Zandi-Nejad K, Mount DB, and Romero MF. Slc26A9 is a highly electrogenic and Na+

transporting

anion exchanger. FASEB J 19: A656, 2005.

70. Plata C, Chang M-H, and Romero MF. Cloning and functional characterization of a zebrafish, electrogenic, sodium

monocarboxylate cotransporter (Slc5A8 / zSMCTe). FASEB J 19: A671, 2005

71. Sussman CR, Chang M-H, Plata C, Angle NM, and Romero MF. Localization, function and gene structure of a zebrafish

electrogenic Na+

bicarbonate cotransporter, NBCe1. FASEB J 19: A646, 2005

72. Choe KP, Kato A, Hirose S, Plata C, Romero MF, Claiborne JB, and Evans DH. NHE3 in an ancestral vertebrate: function

in elasmobranch gills. FASEB J 19: A638, 2005.

73. Fukuda H, Hirata T, Nakamura N, Wakabayashi S, Romero MF, and Hirose S. Molecular characterization and

immunolocalization of a bicarbonate transporter highly and predominantly expressed in rat choroid plexus epithelial cells

(on CD-ROM). Faseb J 19: A#922.923, 2005

74. Gorin MB, Demirci FY, Chang M-H, Mah TS, and Romero MF. A novel missense mutation in the Sodium Bicarbonate

Cotransporter (NBCe1/SLC4A4) in a patient with proximal renal tubular acidosis and ocular pathology.

Am Soc Human Genetics, 2005

75. Sinđić A, Plata C, Sussman CR, Chang M-H, and Romero MF. Localization of Slc26a9 and role of the STAS domain.

FASEB J 20: A839, 2006.

76. Chang M-H, Babcock GT, Hirose S, and Romero MF. Functional characterization & physiology of NBCe1 from a marine

teleost. FASEB J 20: A1233, 2006.


77. Plata C, Sussman CR, Josephs ZM, Chang M-H, Liang JO, and Romero MF. Localization and Functional Characterization

of Two Na+/Monocarboxylate Transporters in zebrafish (zSMCT): electrogenic (zSMCTe/Slc5a8) and electroneutral

(zSMTn/Slc5a12). FASEB J 20: A1234, 2006.

78. Sussman CR, Babcock GT, Plata C, Liang JO, and Romero MF. Cloning and characterization of zebrafish epidermal

growth factor receptor family member, ErbB4. FASEB J 20: A1273, 2006.

79. Sindic A, Huang C, Che D, Ding Y, Romero MF, and Miller RT. Interaction of Kir4.2, a Distal Nephron K+ Channel with

MUPP1, a PDZ Domain-Containing Protein, Results in Increased Cell Surface Expression and Activity of Kir4.2. J Am

Soc Nephrol 2006.

80. Chang M-H, Babcock GT, and Romero MF. Evidence of functional NBCe1 (SLC4A4) dimer assembly. FASEB J 21,

2007.

81. Sindic A, Mount DB, Plata C, Sussman CR, and Romero MF. The rat Slc26a9 anion transporter is located in many

epithelial tissues. Pediatric Pulmonology, 2007

82. Kato A, Chang M-H, Kurita Y, Nakada T, Hirose S, and Romero MF. Functional Characterization of Pufferfish Slc26a6A

and Slc26a6B. Faseb J 22, 2008

83. Plata C, Juárez ME, Vázquez N, Gamba G, and Romero MF. Valproate and Nonsteroidal Anti-Inflammatory Drugs

Reduces the activity of electroneutral (SMTn/Slc5a12) and electrogenic (SMCTe/Slc5a8) Na+/Monocarboxylate

Transporters (SMCT). Faseb J 22: 1202.12, 2008.

84. Plata C, Vázquez N, Romero MF, and Gamba G. Opposite effects of WNK3 and WNK4 upon the Slc26a9 anion

transporter/channel. Faseb J 22: 1202.9, 2008

85. Kurita Y, Nakada T, Kato A, Doi H, Mistry AC, Chang M-H, Romero MF, and Hirose S. Identification of Intestinal

Bicarbonate Transporters Involved in Formation of Carbonate Precipitates to Stimulate Water absorption in Marine

Teleost Fish. Faseb J 22, 2008.

86. Liang J, Manieri A, Kramek H, Kuranovic D, Robinson J, Romero MF. Zebrafish in the Classroom: Building an

Educational Website. Zebrafish meeting, Madison, WI, 2008

87. Chen A-P, Chang M-H, Sindic A, Mount DB, Romero MF. Single nucleotide polymorphisms (SNPs) of human

SLC26A9. FASEB J 23: 796.24, 2009

88. Chang M-H, Holmes HL, Ranatunga WK, Chen A-P, Romero MF. NBCe1 (SLC4A4) functional dimer assembly. Faseb J

23, 2009

89. Lopez A, Vazquez N, Torres N, Zandi-Nejad K, Mount DB, Gamba G, Tovar A, Romero MF, Plata C. SGK1 and Insulin

reduce the activity of mammalian electrogenic Na+/Monocarboxylate Transporters (SMCTe/Slc5a8). Faseb J 23: 797.11,

2009

90. Romero MF, Sindic A, Sussman CR, Chang M-H, Plata C, Chen A-P, Mount DB, Bahler CK, and Fautsch MP. Slc4 and

Slc26 bicarbonate transporter function and localization in the eye. Invest Ophthalmol Vis Sci 50: E-Abstract 3628, 2009

91. Hirata T, Kato A, Cabrero P, Chang M-H, Dow JAT, and Romero MF. Drosophila Slc26a5 functions as a Cl-/Oxalate

exchanger similar to mammalian Slc26a6. J Am Soc Nephrol, 2009.

92. Dow JAT, Cabrero P, Hirata T, Chang M-H, and Romero MF. A simple model for nephrolithiasis: Drosophila Prestin

(Slc26a5) functions as a Cl-/Oxalate exchanger in the gut with kinetics similar to mammalian Slc26a6. J Physiol, 2009.

93. Brin LR, Hirata T, Scileppi EM, Holmes HL, Linser PJ, and Romero MF. Characterization of Anopheles gambiae Slc26a5

and potential role in malaria. Faseb J: 24:1002.25, 2010

94. Hirata T, Kato A, Chang M-H, Hirose S, and Romero MF. Pufferfish Slc26a5 (prestin) exchanges Cl- for oxalate, sulfate,

and bicarbonate. Faseb J: 24:1002.24, 2010.

95. Li Q, Babilonia E, Chang M-H, Kwon C, Mercado A, Mandal A, Romero MF, and Mount DB. A role for C-terminal

cysteines in dimerization of the Slc26a6 anion transporter. Faseb J: 609.13, 2010.

96. Chen A-P, Romero MF. Channel inhibitory region within the STAS domain of human SLC26A9. Faseb J: 1002.7, 2010

97. Hirata T, Cabrero P, Dow JAT, and Romero MF. Drosophila Prestin provides an in vivo model for oxalate kidney stone

formation. J Am Soc Nephrol 21: in press, 2010

98. Chang M-H and Romero MF. NBCe1 (SLC4A4) functions as a dimer and requires both the N- and C-termini. FASEB J

25, 2011

99. Roy-Chowdhury U, Bahler CK, Hann CR, Chang M-H, Romero MF, and Fautsch MP. Additive Effect of Latanoprost-

Free Acid and Diazoxide, an ATP-Sensitive Potassium (KATP) Channel Opener, on Outflow Facility Increase in Human

Anterior Segment Culture Invest Ophthalmol Vis Sci, 2011

Invited Talks:

1. MF Romero, JG Douglas, U Hopfer, and JW Jacobberger. Characterization of a proximal tubular epithelial cell line.

Workshop on the Development of Cell Lines for Hypertension Research, National Heart, Lung, and Blood Institute,

Washington, D.C., February 19-20, 1991.

2. U Hopfer, MF Romero, Z Madhun, JG Douglas. Angiotensin II responses of proximal tubule cells in primary culture and

in a newly generated proximal tubular cell line. XIIth International Congress of Nephrology. Jerusalem, Israel, June,

1993.


3

3

3

3

3. March 10, 1995: “How do Xenopus oocytes move CO2 and H2O ?” Dept. Biology, Thomas More College, Crestview

Hills, KY. Invited by Dr. John Hageman.

1996

4. April 22, 1996: “Expression cloning of the renal electrogenic Na/HCO3 cotransporter, NBC,” Dept Cellular & Molecular

Physiology, Yale University, New Haven, CT. Invited by Dr. Brent Thompson.

5. May 7, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Div

Nephrology, Dept Medicine, Einstein, Bronx, NY. Invited byDr. Victor L. Schuster.

6. May 22, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept

Physiology, Univ. Maryland, Baltimore, MD. Invited by Dr. Paul Welling.

7. May 28, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept

Physiology, Cornell University, NY, NY. Invited by Dr. Henry Sackin.

8. May 30, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Renal

Division Brigham & Women’s Hospital, Boston, MA. Invited by Dr. Steve Gullins.

9. June 27, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept

Molecular Medicine, Beth Israel Hospital, Boston, MA. Invited by Dr. Seth Alper.

10. MF Romero, MA Hediger, EL Boulpaep, and WF Boron. The electrogenic sodium bicarbonate cotransporter: expression

cloning and physiology. Am Physiological Society Conference I: pHysiology of acid-base regulation. Snowmass, CO,

July 12-15, 1996.

11. Sept. 18, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept

Biochemistry and Molecular Biology, St. Louis Univ. St Louis, MO. Invited by Dr. William S. Sly

12. Nov. 11, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Division of

General Medical Sciences, Univ California at Davis, Davis, CA. Invited by Dr. Paul Fitzgerald.

13. Dec. 2, 1996: “The renal electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept

Physiology, University of Arkansas at Little Rock, Little Rock, AR. Invited by Dr. Michael Jennings.

14. Dec. 19, 1996: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept

Physiology and Biophysics, Univ. Alabama at Birmingham, Birmingham, AL. Invited by Dr. Dale Benos.

1997

15. MF Romero, MA Hediger, P Fong, & WF Boron. Expression of the Rat Renal Electrogenic Na/HCO3 Cotransporter

(rNBC). “Latest Advances: Theme I” at the XIV th International Society of Nephrology, Sydney, Australia, May 25-29,

1997.

16. Jan 31, 1997: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept

Physiology and Biophysics, Finch University, The Chicago Medical School, North Chicago, Il. Invited by Dr. Robert

Rokowski.

17. June 16, 1997: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” Dept

Physiology and Biophysics, Case Western Reserve University, Cleveland, OH. Invited by Dr. Richard Eckert.

1998

18. Feb 13, 1998: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning and physiology,” MRC Group

in Membrane Biology, Univ. Toronto and Cell Biology Division , Hospital for Sick Children, Toronto, Ontario, Canada.

Invited by Dr. Reinhart A.F. Reithmeier

19. March 9, 1998: “The renal electrogenic Na/HCO3 cotransporter ,NBC: Expression cloning, physiology, and genomic

frontiers” Dept. Genetics and Center for Human Genetics, Case Western Reserve University, Cleveland, OH. Invited by

Dr. Gregg Mattera.

20. May 19, 1998: “Electrogenic Na/HCO3 cotransporter, NBC: Expression cloning and physiology,” Dept. Pharmacology,

Case Western Reserve University, Cleveland, OH. Invited by Dr. John Nelson.

21. MF Romero and CM Sciortino. Cation and voltage dependence of a rat, renal electrogenic Na/HCO3 cotransporter

(rkNBC) expressed in Xenopus oocytes. Membrane Transport Proteins-Gordon Conference, Tilton, NH, 7/19-24, 1998.

1999

22. Feb. 1, 1999: "Cloning and characterization of electrogenic Na+/HCO

– cotransporters (NBCs)." Physiologisches Instiut

der Universität Zürich, Zürich, SWITZERLAND. Invited by Prof. Dr. Heini Murer


– cotransporters (NBCs)." Abteilung fuer

Allgemeine Zoologie, FB Biologie, Universitaet Kaiserslautern, Kaiserslautern, GERMANY. Invited by Prof. Dr.

Joachim W. Deitmer.


– cotransporters (NBCs)." Zentrum der Physiologie,

Klinikum der J.W. Goethe-Universitaet, Frankfurt/Main, GERMANY. Invited by Prof. Dr. Eberhard Frömter.


– cotransporters (NBCs)." Division of Pediatric

Pulmonology- Cystic Fibrosis Center-Rambow Babies & Children's Hospital. Invited by Dr. Mitch Drumm.

26. May 25, 1999: "Electrogenic Sodium Bicarbonate Cotransporters (NBC's)." Rammelcamp Center for Education and

Research at MetroHealth Medical Center.


3

3

3

3

3

3

27. MF Romero. Cloning and characterization of electrogenic Na+/HCO

– cotransporters (NBCs). Foster Club -Dept.

Physiology, Cambridge University, Cambridge, England, UK; Jan. 27, 1999.—Invited by Prof. Roger Thomas

28. MF Romero. Cloning and characterization of electrogenic Na+/HCO

– cotransporters (NBCs)--an ion transporter of the eye.

Department of Optometry and Vision Sciences, University of Wales Cardiff, Wales, UK; Jan. 29, 1999—Invited by Drs.

Hodgson, Wiggham, and Rimmer.

29. MF Romero. Electrogenic Na+- HCO

– cotransporters (NBCs): acid loader or acid extruder?. Invited Symposium Speaker:

'Acid/Base Transporters in Epithelial Cells', Physiology Society Meeting Manchester, England, UK, March 29-31, 1999.

30. Romero, MF. Na/HCO3 cotransporters in various tissues. Borelli Acd-Base Conference, Anacapri, Italy, Oct. 1-3, 1999.

31. Romero, M. F., D. Henry, S. Nelson, E. P. Jane, P. J. Harte, and C. M. Sciortino. Cloning, Localization and Expression of

a Na+ Driven Anion Exchanger, NDAE1. 1999 American Society of Nephrology " Free communication" in Acid-Base

Transport, November 6, 1999.

32. Romero, MF. Transport pathways in prostate epithelium. In Nonrenal epithelia of the urogenital tract: lessons from the

kidney applied to physiology and pathophysiology. 1999 American Society of Nephrology Basic Science Symposia,

November 8, 1999.

2000

33. Feb 11, 2000: " Cloning and Expression of a Na+

Driven Anion Exchanger, NDAE1." NIH, NHLBI: Laboratory of

Kidney and Electrolyte Metabolism. Invited by Mark A. Knepper.

34. Feb 24, 2000: "Cloning and expression bicarbonate transporters." Dept. Biology, Kenyon College, Kenyon, OH. Invited

by Karen A. Hicks

35. July 28, 2000: "Cloning and Expression of a Na+

Driven Anion Exchanger, NDAE1." Membrane Transport Group, Depts.

Biochemistry and Medicine, University of Toronto, Toronto, Canada. Invited by Dr. Reinhart A.F. Reithmeier.

36. Oct. 13, 2000: Co-chair of Free Communication Session "Transport of proton and base" at the Am. Soc. Nephrology

annual meeting, Toronto, Canada.

2001

37. Jan. 12, 2001: "Cloning and expression bicarbonate transporters." Dept. Physiology and the CIHR Group in Molecular

Biology of membrane proteins, University of Alberta, Edmonton, Alberta, Canada.

38. March 16, 2001: " Na+

coupled bicarbonate transporters - views across Phyla" Invited Symposium Speaker: 'Acid/Base

Transporters in Epithelial Cells', Physiology Society Meeting, Oxford, England, UK.

39. April 2001: "Na+ coupled bicarbonate transporters: amphibians, mammals, and flies --- oh my" Experimental Biology

2001 Symposium - Molecular mechanisms of HCO– transport. Chair and organizier for Epithelial transport and Renal

Sections of Am. Physiol. Soc., Orlando, FL

40. April 27, 2001: "The electrogenic Na+/HCO

– cotransporter, NBC." Renal Research Conference, Renal Division,

Vanderbilt University School of Medicine, Nashville, TN. Invited by David Mount.

41. April 27, 2001: "Na+

coupled HCO–

transport- through the eyes and guts of flies." Membrane Biology Group, Vanderbilt

University School of Medicine, Nashville, TN. Invited by Kevin Strange, Director Anaesthesia Research.

42. June 5-6, 2001: "Na+

coupled bicarbonate transporters: amphibians, mammals, and flies --- oh my" 19th Annual

Symposium on "Ion transport in kidney and liver: a molecular view," GRTM (Membrane Transport Research Group) ,

Montreal, Canada.

43. Aug 26-31, 2001: "Na+

coupled bicarbonate transporters." Symposium “Cell Bicarbonate Transport " 34th

IUPS

Congress 2001. Christchurch, New Zealand.. Invited by Drs. D. C. Macknight and S.L. Alper.

2002

44. March 20, 2002: “Bicarbonate transporters.” Satelite Symposium “Bicarbonate Transport" at 45th Annual Meeting of

the Canadian Society of Biochemistry, Molecular and Cellular Biology (CSBMCB) and Winternational Symposium on

Membrane Proteins in Health and Disease. Invited by Joe Casey and Reinhart A. F. Reithmeier.

45. April 16, 2002: “The gasly details of bicarbonate transporters- a tale of 2 families.” Pharmacology, CWRU, Cleveland,

OH. Invited by Amy Wilson-Delfosse.

46. April 19, 2002: “Bicarbonate transporters-something old and something new. ” Dept. Physiology & Biophysics, Tulane

University, New Orleans, LA. Invited by Dr. Mouhamed Awayda.

47. April 20-24, 2002: “Recent insights into renal acid-base transporters.” Symposium “Renal Acid-Base Transporters"

Experimental Biology 2002.

48. July 11, 2002: “Na+

coupled bicarbonate transporters”Annual Review of Nephrology – Mexico City, Mexico. Invited by

Gerardo Gamba MD PhD.

49. July 11, 2002: “The gasly details of bicarbonate transporters- a tale of 2 families.”Annual Review of Nephrology –

Mexico City, Mexico. Invited by Gerardo Gamba MD PhD.

50. October 18, 2002: “Bicarbonate transporters- new and old.” NIH, NHLBI: Laboratory of Kidney and Electrolyte

Metabolism. Invited by Mark A. Knepper, Chief.

51. December 10, 2002: “Flavors of bicarbonate transporters- old and new.” Dept. Pathology, Emory University. Invited by

Dr. Didier Merlin.


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52. December 10, 2002: “Transport modes of the electrogenic Na+

bicarbonate cotransporter, NBC1.” Dept. Medicine -

Nephrology, Emory University. Invited by Dr. Jeffery Sands, Nephrology Division head.

2003

53. March 21, 2003: “Molecular physiology of bicarbonate transport.” Whitney Laboratory, Univ. FL, St. Augustine, FL.

Invited by Professor Paul J. Linser, Ph.D.

54. March 25, 2003: “Flavors of bicarbonate transporters- something old & something new.” NIH, NHLBI: Laboratory of

Kidney and Electrolyte Metabolism. Invited by Mark A. Knepper, Chief.

55. Aug. 17, 2003- Romero, MF. “Mammalian transporter families” for PharmaConference 2003 – Transporters & Drugs.

Aug. 17-21, 2003, Pontresina, Switzerland. Invited by Matthias A. Hediger, PhD- organizer

56. Oct. 23, 2003: “The gasly details of bicarbonate transporters- a tale of 2 families.” Renal Electrolyte Division, Univ.

Pittsburg, Pittsburg, PA. Invited by Thomas R. Kleyman, MD.

57. Nov. 16, 2003 - Romero, MF: “Bicarbonate transporters.” Am. Soc. Nephrol. 2003 Symposium – New Insights to Acid-

Base Physiology. Invited by Jeff Sands, MD, organizer.

2004

58. 2004: “The tricks and disguises of bicarbonate transporters.” Dept. Physiology & Biophysics, Wright State University.

Invited by Drs. Tom Brown & Peter Lauf.

59. April 17, 2004: “In the beginning …. There was the cell.” APS Refresher Course on “Cellular Homeostasis.” Organizer

and Symposium Chairperson.

60. April 17, 2004: “Generation of membrane potential.” APS Refresher Course on “Cellular Homeostasis.” Replacement for

Dr. Steve Wright.

61. May 27, 2004: “Sodium/bicarbonate cotransporters and new bicarbonate transporters in the kidney” for Japanese Society

of Nephrology, May 27, 2004, Utsunomiya, Japan. Invited by Prof. Hitoshi Endou (Kyorin University, Tokyo)- organizer.

62. July 18-25, 2004: “NBCe1 - lessons from soluble domains and evolutionary variation.” Biophysical and Biochemical

Properties of Ion Channels in Epithelia Symposium at Telluride Science Research Center, Telluride, CO. Speaker - Invited

by Drs. Thomas Kleyman and John Cuppoletti.

63. Dec 3, 2004: "Ion, solute and acid-base transport physiology of the Na+

coupled monocarboxylate cotransporter SLC5A8 -

a renal perspective." Abbott Laboratories.

2005

64. Feb 2, 2005: “Bicarbonate and acid-base transporters in acid-base balance.” Dept. Molecular & Cellular Physiology,

University of Cincinnati, Cincinnati, OH. Invited by Dr. Marshall Montrose.

65. Feb 28- March 3, 2005: “Electrogenicity of Cl- and HCO3 transport by the SLC26 transporters” for Novartis (formerly

Ciba) Foundation Symposium "Epithelial anion transport in health and disease: the role of the SLC26 transporters family,"

London, U.K. Invited speaker.

66. March 4, 2005: “Overview of the SLC26 family and associated diseases” for “Epithelial anion transport in health and

disease: the role of the SLC26 transporters family.” Sponcered by Novatris and the Physiology Society. Invited speaker.

67. March 9, 2005: Bicarbonate transporters – old friends and new twists. Dept. Integrative and Molecular Physiology,

University of Michigan at Ann Arbor. Invited by Dr. John Williams.

68. March 28, 2005: “Electrogenic Slc26 Anion ‘Transporters’ – New Molecular and Physiologic Twists for Epithelia” Dept.

Cell Biology & Physiology, University of Pittsburgh. Invited by Dr. Raymond Frizzell.

69. May 2, 2005: “Sodium monocarboxylate cotransporters- Cancer, fish, frogs and solute transport.” Physiology &

Biophysics, CWRU. Invited by Dr. George Dubyak.

70. Aug. 29, 2005: “Bicarbonate transporters – physiology insights from molecular physiology” Dept. Physiology, Tulane

University. Invited by Dr. L. Gabriel Navar. Postponed due to Hurricane Katrina

71. September, 19-22, 2005: “Molecular Physiology of chloride exchangers (SLC4 and SLC26)” for the First

International meeting on Physiology and Pathology of chloride transporters and channels, Soria, Spain. Invited

speaker by Dr. Gerardo Gamba.

72. Fall 2005: “Acid-base transport from humans and fish,” Laboratory of Pharmacology & Chemistry and Laboratory of

Molecular Toxicology, National Institute of Environmental Health Sciences., Research Triangle Park, NC. Invited by Drs.

Leo T. Burka and John Pritchard.

73. Nov 11, 2005, ASN 2005: “Electrogenic Na+

Bicarbonate cotransport” for ASN Symposium “Sodium cotransporter

protein families: Molecular biology and regulation.” Invited speaker by Dr. Mark Zeidel.

74. Dec. 15, 2005: “Na+

Bicarbonate cotransporters: biophysics, evolution and human disease” Dept. Physiology &

Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN. Invited by Dr. Gary C. Sieck.

2006

75. Jan. 19, 2006: “Electrogenic Na+

bicarbonate cotransporters in epithelia: physiology, evolution and human disease” Dept.

Anatomy & Physiology, Kansas State University, Manhattan, KS. Invited by Dr. Daniel Marcus.

76. Jan. 27, 2006: “Electrogenic Slc26 Anion ‘Transporters’ – new molecular and physiologic paradigms for epithelia”

Pediatric Pulmonary/CF Research Center Seminar at CWRU, Cleveland, OH. Invted by Dr. Calvin U. Cotton.


77. Feb 20, 2006: “Electrogenic Na+

bicarbonate cotransporters in epithelia: physiology, evolution and human disease,” Dept.

Anatomy & Cell Biology, Univ. Pennsylvania School of Dental Medicine, Philiadelphia, PA. Invited by Dr. Ed Macarak.

78. Feb 23, 2006: “Electrogenic Na+

bicarbonate cotransporters in the kidney and epithelia: physiology, evolution and human

disease.” Nephrology Hypertension Research Conference, CWRU. Invited by Mike Simonson.

79. Nov. 4, 2006; 21st annual Ohio Physiological Society Meeting, Kenyon College, Gambier, OH: “Frogs, fish and flies to

study ion transporters”. Invited by Dr. Chris Gillen.

80. Dec. 19, 2006: “Bicarbonate transporters in the kidney and epithelia: physiology, evolution and human disease.”

Nephrology Grand Rounds, Mayo Clinic. Invited by Vicente Torres, M.D., Ph.D.

2007

81. Jan. 26, 2007: “Electrogenic bicarbonate transporters in the kidney and epithelia: physiology, evolution and human

disease.” Physiology & BME seminar, Mayo Clinic. Invited by Armando Manduca, PhD

82. March 8-11, 2007- APS Professional Skills Course: Making Scientific Presentations: Critical First Skills, Bethesda, MD;

“Designing a Great Poster.”

83. Aug 13, 2007: BioMedical Transporters 2007 Conference, Berne, Switzerland: “NBCe1, the molecular basis of proximal

renal tubular acidosis and glaucoma – the kidney, flies and fish & back again.” Invited by Dr. Matthias A. Hediger.

84. Aug 17, 2007: “Structure & function of bicarbonate transporters- lessons from human disease and evolution” Institute fur

Biophysik, Uni. Stuttgart, Stuttgart, Germany. Invited by Prof. Dr. Stephan Nuβberger.

85. Oct 22, 2007: “Electrogenic bicarbonate transporters in the kidney, eye and epithelia: physiology, evolution & human

disease.” Opthamalogy Grand Rounds, Mayo Clinic.

2008

86. April 2008; EB Featured topic: “Gut of fly, kidney and cake of fish, stomach of man & egg of frog – a witch’s brew of GI

epithelial transport.” Invited by Drs. Didier Merlin and Pradeep Dudeja.

87. June 7, 2008: ROCK SOCIETY 2008 “Slc26 Proteins and Oxalate Transport.”

88. July 11, 2008: “Transporter Biology & Physiology: Romero lab overview.” Physiology & BME seminar series.

89. Sept 5-8, 2008: Epithelial Solute Transporters and Acid-Base Regulation, Le Chateau Montebello, Quebec, Canada “The

SLC26 family of anion exchangers.” Invited by Drs. Steve Perry, Bill Harvey and Nathan Nelson

90. Sept 24-29, 2008: International Congress of Eye Research (ICER), Beijing, China: Physiology & Pharmacology session.

“Slc4 and Slc26 anion transporters in epithelia.” Invited by Alan Marmorstein, PhD

91. Nov. 11, 2008: Boulpaep Symposium- Yale University. “Evolving and shifting anion transport modes with the Slc26

anion “transporters”” Invited by Michael J. Caplan, MD, PhD (Chair, Cell & Molec. Physiology).

2009

92. July 10, 2009: “Transporter Biology & Physiology.” Biochemistry & Molecular Biology- SURF presentation.

93. Sept. 25, 2009: “The Unbearable Lightness of Anion Transporters - the common thread of acidosis, cystic fibrosis,

malaria, glaucoma and kidney stones.” Physiology & BME seminar series.

2010

94. March 19, 2010; “Oxalate transport, kidney stones and Slc26 transporters” Mayo O’Brien Center Research Conference.

Invited by John Lieske, MD, O’Brien Center Director.

95. April 13, 2010; “Microelectrodes to determine ion and solute transport in cells – tools for acidosis, cystic fibrosis, malaria,

glaucoma and kidney stones” Design of Medical Devices Conference, Minneapolis, MN. Invited by Tianhong Cui, PhD.

96. May 17, 2010; Tokyo Institute of Technology. Invited by Dr. Shige Hirose, Dean.

97. May 19, 2010; Laboratory of Biotechnology, Shizuoka University. Invited by Dr. Enoch Park.

98. May 19-21, 2010; Morioka, Japan, 87th Annual Meeting of the Physiological Society of Japan: Invited by Manabu

Kubokawa, MD, PhD, Vice President of Physiological Society of Japan.

99. July 9, 2010; “Epithelial bicarbonate transporters – insights to acidosis, glaucoma, kidney stones and malaria” Physiology

& BME, Mayo Clinic. Invited by Dr. Armando Manduca

100.September 27, 2010; “Oxalate transport, kidney stones and Slc26 transporters” Physiology and Functional Genomics,

University of Florida College of Medicine. Invited by Drs. Debbie Scheuer and Charles Wingo

2011

101.Jan 18, 2011; “Bicarbonate transporters – Models to study kidney disease, glaucoma and parasite control” Biochemistry

and Molecular Biology, Mayo Clinic. Invited by Dr. Mark McNiven

102.March 1, 2011: “In vivo Drosophila model of Ca-oxalate nephrolithiasis - a search for genetic pathophysiology”

Nephrology & Hypertension Grand Rounds.

103.May 20, 2011: “Mayo Translational PKD Center - Model Systems Core” External Advisory Site visit.

104.May 24, 2011; “PhD research funding: illusions, realities and possibilities” Mayo Postdoctoral Association.

105.June 28, 2011: “Epithelial bicarbonate transporters – insights to renal tubular acidosis, glaucoma, cystic fibrosis and

kidney stones” Molecular, Cell & Systems Biology, Univ. Glasgow, UK. Invited by Prof. Julian AT Dow.


106.July 1-4, 2011; “Drosophila model of kidney stones: function and knockdown of Slc26a5 (prestin)” Glasgow conference

honoring Prof. Bill Harvey. Invited by Klaus Beyenbach, PhD.

107.July 3, 2011; “Bicarbonate transporters in the Drosophila and Anopheles” Satelite meeting for Soc. Exp. Biol. Glasgow

conference. Invited by Julian Dow, D.Sc.

108.July 19, 2011; “How we try to understand kidney stone disease” MF Romero and J Lieske. Presentation of Mayo O’Brien

Research Center to Mayo SURF students

109.July 21, 2011; “Flies to humans, kidney disease, cystic fibrosis, glaucoma and malaria: Roles of bicarbonate transporting

membrane proteins” Biochemistry and Molecular Biology seminar. Invited by Jennifer Westendorf

110.July 22, 2011; “Bicarbonate and Solute Transporters in Epithelial – Why Should You Care?” Physiology & biomedical

Engineering Seminar, Mayo Clinic College of Medicine. Invited by Dr. Armando Manduca.

111.July 29, 2011; “O’Brien Urology Center – Pilot Projects and Supplements” O’Brien Urology Research Center update.

112.August 29-30, 2011: “Bicarbonate transporters in the Drosophila and Anopheles: possible models for human disease”

icarbonate transporters Symposium-15 yrs. Aarhaus, Denmark. Invited by Drs. Ebbe Boedtkjer and Christian Aalkjaer.

113.Oct 3, 2011. “Stoned Flies, cystic fibrosis and glaucoma: Roles of bicarbonate transporting membrane proteins”

Physiology & Biophysics, Case Western Reserve University School of Medicine; Invited by Walter F. Boron, MD, PhD

(Chair)

114.Nov 18, 2011: Symposium in honor of Ulrich Hopfer, MD-PhD. Physiology & Biophysics, Case Western Reserve

University School of Medicine; Invited by Walter F. Boron, MD, PhD (Chair)

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