Micro Raman Spectrometer - newagein.com

Micro Raman Spectrometer

New Age Instruments & Materials Pvt. Ltd.

Model: NAµRAMS-01

Simultaneous / Multifunctional Analysis

Raman MeasurementsLuminescence MeasurementsLaser Reflection & Transmission Measurements Spectral and Polarization measurements

NAµRAMS-01

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Confocal Detection Principle

Confocal Laser Scanning Raman Microscope hasbecome a widely recognized research instrumentin recent years. Confocal microscopy offersseveral advantages over conventional wide-fieldoptical microscopy, including the ability to controldepth of field, elimination or reduction ofbackground information away from the focal planeand the capability to collect serial optical sectionsfrom thick samples. The image of the extendedsample is generated by scanning the focused laserbeam across a defined area.

The pinhole aperture rejects the residual scatteredrays originated from any out-of-focus points on a sample.


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We have created the instrument that is right for you

High spectral resolution

Spatial resolution: less than 500 nm (Z), 200 nm (X, Y) -1 Spectral resolution: ~ 0.25 cm

Wavelength accuracy in spectrum with CCD detector:0.005 nm (1800 l / mm)

Applications

SemiconductorsHigh spatial resolution Raman confocal microscopy can provide

information on dopant concentrations and stress distribution in

semiconductor materials.

BiologyRaman spectroscopy allows easy visualization of cellular components

with minimum perturbation.


520 mm focal length imaging spectrograph offers ahighly configurable platform, with advanced usercontrols to always access the very best spectralperformance for routine measurements and moredemanding optical setups.

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PharmaceuticsConfocal Raman spectroscopy allows chemical compounds and

molecular conformers in various drugs to be identified and their

distribution mapped with high spatial resolution.

GeologyConfocal Raman microscopy is an excellent technique for

characterization of minerals, detection of components distribution

and their phase transitions.

CosmetologyConfocal Raman microspectroscopy is a promising technique which

enables measuring the skin care products as well as their penetration

capability.

ForensicsApplication areas include identification of unknown substances,

different types of fibers, glasses, paints, explosive materials, inks,

narcotic and toxic substances, proof of authenticity of documents.

Material scienceConfocal Raman offers excellent spatial resolution for characterization

of materials (superconductor, polymers, coatings, composites, carbon

nanotubes, graphene, etc.).

Heritage and Art, GemologyRaman spectroscopy allows identfication of pigments and binders

used in paintings. The spectroscopic analysis of archaeological

samples (ceramics, glasses, etc.) provides information on their origin

and history. Raman technique allows rapid identfication of colored

stones, natural and synthetic diamonds.


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Raman megapixel image for 3 sec

Fully automated system with up to 5 integrated lasers

Major features

The smaller amount of illuminated pixels on the CCD matrix leads to

the smaller dark counts and the higher spectral resolution.

High spatial resolution and sensitivity

The highest spectral and imaging resolution withspecially designed spectrometer

Specially designed imaging spectrometer incorporates many features

that make it ideal for confocal Raman measurements. The image of

pinhole is projected to a multichannel detector without any aberrations.

Spectral resolution of NAµRAMS-01 with an Echelle grating-1

is 0.25 cm .


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Optical and confocal fluorescence and Raman images (B and F)

corresponding to beta carotene, fluorescence images (C and G)

corresponding to proplastids.

Euglena algae cells

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Confocal laser and Raman microscopy and opticalmicroscopy of the algae cell

Optical image

25X25 microns

Confocal

laser image

Raman map of Beta

Carotene band

Fluorescence

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Recognition of living and dead cells

Raman spectra of the cell nucleus have significantly lower peaks in dead

cells as compared to living cells. A decrease in intensity is observed in

almost the full range, especially at the peaks of 783 and 827 1/cm and at

the peaks of 1003 and 1337 1/cm, which corresponds to the breaking of the

internal phosphodiester bonds in the DNA molecules and a decrease in

the level of protein expression.


However, in the nuclei of dead cells, lines increase by 1578 1/cm and new

Raman peaks appear at 1607 or 1114 1/cm, which corresponds to Phe and

Try, respectively. These results indicate the degradation of DNA molecules

and the presence of new aromatic amino acids rich in proteins, which

usually occur during cell apoptosis

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Maleria-infected erythrocytes

AFM image of an erythrocyte and a 3x cluster map of the same

cell (above) plotted from the CR spectra. Averaged Raman

spectra of two clusters associated with hemoglobin

(green) and hemozoin (blue) are presented below the maps.


Hemozoin is an iron-containing pigment present in parasites that cause

malaria in humans. Raman spectroscopy can identify hemozoin in

erythrocyte cells.

C

AFM topography Raman map

Hemozoin

Hematoglobulin

Raman spectra

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FLIM mapping of e-coli


FLIM mapping of

525-540 nm band

(top) and

fluorescence

intensity (left).

Decay curve on the bottom left image and fluorescence spectrum

on the bottom right one. Different FLIM signals come from different

fluorescent proteins, which produced by e-coligenetically modified

in different ways. Spectrum shape is very similar. Intensity

and lifetime is different. FLIM provides ufficient information to identify

different proteins in bacteria.

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The distribution of proteins, lipids, DNA and RNAin HeLa cells

Proteins and lipids were observed in the CARS mode at their characteristic-1 -1vibrations of 2930 cm and 2840 cm , respectively. Nucleic acids, stained

with acridine orange, were acquired in the red (RNA = green) and green

(DNA = blue) fluorescence channels in TPEF mode.

FLIM mapping of e-coli

Lifetime map

525-540 nmFluorescence

525-540 nm

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High optical throughput for enhanced sensitivity

People with little or no experience

in Raman spectroscopy can use

NAµRAMS-01 . The system is highly

modular and fully automated.

Up to 5 lasers can be used.

Silicon 4th order sensitivity.

-1The 4th order Silicon band at 1940 cm can be observed in less than

one minute using a low intensity laser.

The lasers can be switched from

one to another by just one click.

2D / 3D images can be acquired rapidly.

Fully automated

Motorized control for laser power, beam diameter, polarization

orientation, pinhole size and grating is provided.


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Micro Raman Spectrometer NAµRAMS-01 can achieve:

Low frequency Raman shift measurements (down to-1

5 cm ) with Bragg Super-Notch filters

True confocal design High spatial resolution

- lateral resolution close to theoretical limitation

Laser wavelength, nm Objective XY - plane resolution, nm

325

532

633

785

40x, NA = 0.5

100x, NA = 0.9

100x, NA = 0.9

100x, NA = 0.9

325

275

320

390

NAµRAMS-01 can take high

definition Raman images (λ = 514 nm, 100x,

NA = 1.4).

- axial resolution (in depth direction,

100x, NA = 0.9)

Laser wavelength, nm Z (axial) resolution, nm

325

532

633

785

500

560

660

800


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Axial resolution of 325 nm (λ = 325

nm, 40x, NA = 0.5).

Wide Raman shiftmeasurement range

Laser wavelength, nm -1

Wavenumber range, cm

325

532

125 - 8000

50 - 8000

633

785

50 - 6000

40 - 2800

Low-frequency Raman shift measurement range can be expanded

using Bragg notch filters.

Low-frequency Raman bands of

sulfur (lower than 250 cm-1, 633

nm laser)

Megapixel Raman imagefor 3 sec

True confocal design

High spatial resolution


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Micro Raman Spectrometer NAµRAMS-01 provides the acquisition of

two images within a single scan: a Rayleigh image (using laser light

reflected from a sample) and a spectral image by Raman scattering.

Ultrafast imaging option allows to get confocal image in 3 sec

(3 μs/pixel).

NAµRAMS-01 uses fast beam scanning by galvano mirrors.

Layout of galvano mirror scanner module allows mapping with no

intensity losses from image center to its edges.

Rayleigh (1000 x 1000 pixels, time per 1 pixel is 3 μs) and Raman

(1000 x 1000 pixels, time per 1 pixel is 43 μs) images of Granite Gneiss

India. Anatase distribution.

NAµRAMS-01 system can be used with a number of different

detectors.

Fast imaging mode with EMCCD / CCD

Up to three detectors can be used simultaneously. Proprietary algorithm

for taking high speed of Raman imaging with fast spectral CCD (EMCCD)

is offered.


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NAµRAMS-01 software ““NARscan”” with powerful analytical

functionality is designed for hardware operating, data acquisition and

data analysis.

Data Acquisition and DataAnalysis software

2D and 3D image creation

Autofocus control during mapping

Automatic background subtraction, cosmic ray removing, peak

shift imaging, etc.

Support for external spectral databases

Data export to popular file formats

Intuitive user-friendly interface

Compatible with Windows XP, Vista, 7


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Raman image of Silicon / SiO2 sample.

Si distribution (500 x 500 pixels, time per

pixel is 5 ms).

Uniform, large size scanning area of a galvanic scanner module:

Fully automated system Softwarepackage with powerful analyticalfunctionality

Ultrawide field Raman imaging

150 μm x 150 μm (objective lens 100x)



Automatic XY stage can be used for ultra-wide field imaging.

The panoramic image (hyper image) by automatic stitching of a series

of images obtained with the use of galvanic scanner.

High precision spectrometercalibrationModel: NAµRAMS-01 is equipped with a

neon lamp (option) for spectral calibration.

Calibration is possible at any wavelength by

one click in the control software.

More capabilitiesmicroscope can be equipped with a heating or cooling stage,

vacuum or high pressure cell

fiber optics probe for remote measurements


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Our Other HAWK RAMAN Products

HAWK RAMAN PORTABLEThis portable Raman System is under 2.5 Kgs.Designed 1.5 meter fiber coupled Raman Probe.System is developed to keep in mind ease andportability with no compromise in measuringcapabilities. it has 7-inch-wide touch screen.

HAWK RAMAN FREE SPACEFor applications where sample is not in direct accessand user wants to conduct identification in free space.

HAWK RAMAN MICROThe Hawk Raman Micro System is designed keeping inmind the users that are interested in micron level spatialresolution of their raman sample.This system canprovide analysis on very small feature of their sample.

HAWK RAMAN HANDHELDA field-ready invaluable identification tool weighing only 800gms. Designed for very quick check and forensic analysis bysafety personal and first responders including Law enforce-ment personnel, Bomb squads, Customs and Border Patrol.

HAWK RAMAN COOLEDWe are offering TEC Raman for demanding applicationslike Pharmaceutical analysis of samples where higherexposure time are required, a cooled sensor (Δ65°C)helps in giving excellent signal to noise ratio makingit possible to measure even the slightest Raman signalsand detect subtle sample differences.

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NAµRAMS Confocal microscopes product line

NAµRAMS E200

NAµRAMS M350

NAµRAMS S120

NAµRAMS-01 NAµRAMS CARS

New Age Instruments & Materials Pvt. Ltd

# 12 – 15, IInd Floor, Apna Enclave Shopping Complex,Railway Road, Gurgaon – 122001, Haryana, IndiaTel. : +91-124-4086513 - 516, 4112517E- Fax : +91-11-47618018 | E-mail : [email protected] : www.newagein.com

Our R&D team has the core competence in the field of Laser, Optics,Spectroscopy, Optoelectronic, Instrumentation, Mechanical andapplication software designing. We have successfully executed manyprojects for Industry, Government labs for both field and lab basedapplications.

Our Company has been recognized by DSIR for In-House Research &Development Activities in India. Our company is also registered withMSME, NSIC and are also in various government labs like DRDO, ISRO,DAE, IITs, CSIR and universities. We are always looking for newtechnologies and solutions that can help to innovate.

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