7/30/2019 Microbiology Quality Control

1/31

Saeeda. ChandioSenior technologist II

Microbiology

7/30/2019 Microbiology Quality Control

2/31

Process or system for monitoring thequality of laboratory testing, and theaccuracy and precision of results

Routinely collect and analyze data fromevery test run or procedure

Allows for immediate corrective action

7/30/2019 Microbiology Quality Control

3/31

IQC refers to the set of proceduresundertaken by the laboratory staff for the

continuousand immediate

monitoring of laboratory work in order todecide whether the results are reliable

enough to be released.

7/30/2019 Microbiology Quality Control

4/31

Quality of specimen Validity of test method Performance of test procedure, reagents

and media Reports and interpretation Staff Equipment

7/30/2019 Microbiology Quality Control

5/31

Lab Tech-The person who performs testing

Supervisor-The person who is responsible forday-to-day activities, training, delegation ofwork

Director-The person who is responsible forentire seamless operation, planning, andcontrol of all activities

Ministry of Health-Place responsible forinfrastructure, man power, and resources.

7/30/2019 Microbiology Quality Control

6/31

Must be trained to perform the tests

Follow SOPs

Must run QC samples

Maintain all the record up to date Inform the Supervisor of any problems---

immediately

Corrective actions taken by them must benoted down and any implications on othersamples

7/30/2019 Microbiology Quality Control

7/31

Train the Lab tech for the assigned job Provide periodic training to the lab techs

Prepare the controls and check the values

Prepare and update the SOPs -easy tounderstand

Keep record of Equipment Maintenance,problems, and repairs

Keep records of training, SOPs, equipment,etc.

Ensure all Lab tasks on time

7/30/2019 Microbiology Quality Control

8/31

The Quality of the specimen

The Performance of test procedures,

reagents and media

7/30/2019 Microbiology Quality Control

9/31

Appropriate specimen

Collection method

Delivery system

7/30/2019 Microbiology Quality Control

10/31

Record amount prepared

Source

Lot number

Sterilization method

Preparation date and Expiration date

Prepared by

7/30/2019 Microbiology Quality Control

11/31

Check:

Sterility

Ability to support growth

Indicative, selective, or inhibitory

Characteristics of the medium

Biochemical response

7/30/2019 Microbiology Quality Control

12/31

Test QC organisms with each new batch orlot number

Check for growth of fastidious organismson media of choice incubate at time andtemp recommended

RECORD Results on Media QC form

7/30/2019 Microbiology Quality Control

13/31

Biological indicators (BIs)

Chemical indicators (CIs)

Mechanical monitoring

7/30/2019 Microbiology Quality Control

14/31

7/30/2019 Microbiology Quality Control

15/31

If you have not documented it,

you have NOT done it

If you have not documented,

it is a RUMOUR !!!

7/30/2019 Microbiology Quality Control

16/31

Staining and Microscopy Culture Media

Incubation

Organism Identification Antimicrobial Susceptibility Testing

7/30/2019 Microbiology Quality Control

17/31

Dating when received and when opened Weighing

Dissolving

Sterilizing Storage of prepared media

Addition of enrichments [e.g. Blood]

Sterility Testing

Incubation of inoculated media

7/30/2019 Microbiology Quality Control

18/31

Agar Plates Haemolysis controls

Selective media controls

Liquid broths

Positive and negative reactions

Therefore Stock Cultures

7/30/2019 Microbiology Quality Control

19/31

Organisms to be maintained must beadequate to check all media and test systems.

E. coli MacConkey, XLD, susceptibility

tests Staphylococcus aureus Blood agar,

Mannitol Salt, susceptibility tests Neisseria gonorrhoeae chocolate,

Thayer-Martin

7/30/2019 Microbiology Quality Control

20/31

Gram stain QC Use gram positive and gram negative

organisms to check daily ZN QC

Use positive and negative AFB slide withevery batch stained. Other Stains

Check as used positive and negativereactions

Reagents Check as used with known positives and

negatives.

7/30/2019 Microbiology Quality Control

21/31

7/30/2019 Microbiology Quality Control

22/31

7/30/2019 Microbiology Quality Control

23/31

7/30/2019 Microbiology Quality Control

24/31

7/30/2019 Microbiology Quality Control

25/31

7/30/2019 Microbiology Quality Control

26/31

Storage of antibiotic disks

CLSI Method

Detailed QC Procedures to ensurereproducibility of results

0.5 Mcfarlands standard

Depth of agar!!

7/30/2019 Microbiology Quality Control

27/31

7/30/2019 Microbiology Quality Control

28/31

Many organisms have predictableantimicrobial test results

Staphylococcus spp. are usuallysusceptible to vancomycin

Streptococcus pyogenes are alwayssusceptible to penicillin

Klebsiella pneumoniae are resistant to

ampicillin

7/30/2019 Microbiology Quality Control

29/31

If you encounter an unusual pattern rule out error by checking identification of

organisms

repeat antimicrobial susceptibility test

Report if repeat testing yields same result,or refer the isolate to a reference laboratory

for confirmation

7/30/2019 Microbiology Quality Control

30/31

Clinician should be notified about panicvalues immediately. Panic values are lifepotential-threatening results,

Positive LPA

Positive gram stain for CSF

Positive KLB

Positive blood culture.

7/30/2019 Microbiology Quality Control

31/31

thanks