Mini Atlas March 2013b

7/28/2019 Mini Atlas March 2013b

1/28

1 LLTECH 2011

LLTech

LightCT Scanner

Atlas of images

March 2013


2/28

2 LLTECH 2011

BREAST AND LYMPH NODES


3/28

3 LLTECH 2011

Ductal Carcinoma In Situ (DCIS)

Enlarged lobule

Duct with necrosis

Histology and LightCT images reveal the enlarged abnormal lobules and ducts characteristic of DCIS

Assayag et al, TCRT Express 2013 (in press)


4/28

4 LLTECH 2011

100m

Highly scattering

thin trabeculae

aspect: fibrous

tissue in the

tumourous part

Grainy medium

scattering aspect:

healthy fibrous

tissue

Invasive adenocarcinoma: nodular tumor

Interface of the tumourous and normal

appearing part :

Grey zones corresponding to foci

of carcinomatous cells

Tumour margin

500m

500m

Courtesy of Hpital Tenon, Paris, France

Assayag et al, TCRT Express 2013 (in press)


5/28

5 LLTECH 2011 LLTECH 2011

Nodules containing

lymphoid tissue

Normal Sentinel Node

Adipocytes in hilum

Capsule Grieve et al Proc SPIE 2013


6/28

6 LLTECH 2011

Lymphoid zone

Invaded zone

Invaded Sentinel Node

Bright white aspect,

highly scattering dense

collagen meshwork

Grieve et al Proc SPIE 2013


7/287 LLTECH 2011

Pre-clinical study statistics

75 breast samples were imaged with LightCT and compared with histology

Blinded analysis by two pathlogists lead to sensitivity of 94% and 90 % and specificity of 75%

and 79% respectively

45 sentinel nodes were imaged with LightCT and compared with histology

Blinded analysis by one pathologist lead to sensitivity of 93% and specificity of 90% Second look analysis of the data set lead to sensitivity of 100% and specificity of 97%

Conclusions:

Sensitivity and specificity of LightCT images approach histology and can surpass frozen

section analysis

There is a learning curve for pathologists to correctly read LightCT images


8/288 LLTECH 2011

BRAIN AND SPINAL CORD


9/289 LLTECH 2011

Neuron cell bodies (dark dots)

Axons/myelin fibers

Capillary

Cortex

White matter

Axon bundles

LightCT imaging of human epileptic brain and cerebellum identifies myelinated

axon fibers, a subpopulation of neuronal cell bodies and CNS vasculature.

Varlet et al 2013(submitted)

50 m

50 m

80 m

500 m

50 m

80 m

50 m


10/2810 LLTECH 2011

CA4 field

Hippocampal sulcus

Alveus

Sub-Ependymal

zone

CA1 field of the cornus ammonis

Stratum radiatus

of the CA1 field

Stratum granulosum of the dentate gyrus

Pyramidal neurons of the CA4 field

responsible for memory

Hippocampus: Coronal section (left side)

900 m

40 m

40 m

80 m

80 m


11/2811 LLTECH 2011

Choroid Plexus Papilloma : Xanthomatous changes

Characteristic cauliflower-like masses

Empty papilla

Papilla filled with blood

Single layer of plexus cellsRare (0.5%) benign tumors

Diagnosis possible directly on FF-OCT imageVarlet et al 2013 (submitted)

50 m

50 m

20 m150 m

50 m

50 m

20 m


12/2812 LLTECH 2011

LightCT images allow the distinction of meningiomas

from meningeal haemangiopericytoma

Collagen bundles

Whorls

Calcification

Collagen balls

Meningioma psammoma diagnosis possible directly on FF-OCT image

80% of meningiomas are benign.

Histologically, meningioma cells are relatively uniform, with a tendency to encircle one another, forming

whorls and psammoma bodies (laminated calcific concretions). They have a tendency to calcify and are

highly vascularized. Areas of fibrosis may be present.Varlet et al 2013 (submitted)

50 m

10 m

10 m500 m


13/2813 LLTECH 2011

DERMATOLOGY AND COSMETICS


14/2814 LLTECH 2011

Epidermis

Collagen

Pilosebaceous

unit

Sweat gland

Adipocytes

b

a

c

e

d

500 m

Normal skin morphology: vertical section

Dalimier and Salomon Dermatology 224, 84-92 (2012)


15/2815 LLTECH 2011

Skin vertical reconstruction from en-face slicing for

layers assesment and measurement

Stratum corneum

Stratum spinosum

Dermis

Papillary melanin capsBV: Blood vessels

BV

BVBV

KNKN KN

KN: Keratinocyte nuclei

50 m

50 m

Stratum corneum

Stratum spinosum

Dermis

Keratinocyte nuclei

Normal skin

Skin model



16/2816 LLTECH 2011

3D reconstruction from en-face slicing

Wrinkle Stratum corneum Stratum spinosum

Epidermis

800 m x 800 m

Dermis

Dermal papilla Collagen

800 m x 800 m

Epidermis and dermis separated by sodium bromide

Blood vessel

50 m

Reconstructed vertical slice

50 m

Reconstructed vertical slice



17/2817 LLTECH 2011

Adipocytes

9 m depth 27 m depth 60 m depth

Reconstructed

depth slice

200 m

50 m

200 m 200 m



18/2818 LLTECH 2011

Skin pathologies: basal cell carcinoma discrimination

at structural level

Dense peritumoral stroma



19/2819 LLTECH 2011

Zoom on basal cell carcinoma:

discrimination at cellular level

200 m

100 m

Peritumoral stroma

High cell density

Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland


20/2820 LLTECH 2011

CORNEA


21/2821 LLTECH 2011

Surface epithelium Intermediary epithelium Basal epithelium

Bowmans layer Stromal keratocytes Endothelial cells

Cross sectional view En face views

Normal human cornea

100 m 100 m

LightCT imaging reveals the structures of normal cornea: epithelium, Bowmans layer, stroma, Descemets membrane,

and endothelium.

Use of LightCT in Eye Banks would allow graft cornea integrity to be assessed prior to transplant.Current practice examines only the epithelial surface and endothelial cells under a traditional specular microscope.


22/2822 LLTECH 2011

Subepithelial fibrosis

Intraepithelial edema

LightCT cross section LightCT en face

Cornea with bullous keratopathy

Sub-epithelial

fibrosis

Optovue OCT in vivo on patientHistology

Histology, conventional OCT and Light CT views of the same region are compared.

The improvement in resolution with LightCT compared to conventional OCT is appreciated.

Pathological areas of subepithelial fibrosis and intraepithelial edema are identified.

Bowmans layer is clearly visible under the area of fibrosis.

150 m 150 m 100 m

Ghouali et alARVO (2013)


23/28

23 LLTECH 2011

PROSTATE


24/28

24 LLTECH 2011

1 mm

Human prostatic glands with hyperplasia


100 m

Histology of a similar gland (different sample)

Zoom on gland


25/28

25 LLTECH 2011

3D imaging of glands

Stack of 150 images with 1 m step

50 m

50 m

50 m

sagittal

coronal

oblique

Sagittal reconstruction

Coronal reconstruction

Oblique reconstruction



26/28

26 LLTECH 2011

Tumorous glands

200 m

Courtesy of Hpital Cochin, Paris, France


27/28

27 LLTECH 2011

Conclusion

Full-field OCT does not require tissue preparation, nor staining of any kind

Creates images within minutes using a non-destructive process

Offers a 1 m cellular resolution in 3D

Reveals structural and cellular information of normal and pathological tissue Mosaicing allows fast visualisation at various scales

En-face high-resolution imaging allows vertical and 3D reconstruction

Possible applications for biobanking, cancer detection, digital pathology


28/28

Contacts

LLTech, Inc.

103 Carnegie Center Drive

Suite 300

Princeton, NJ 08540, USA

Phone: +1 609 995 3506

LLTech

Ppinire Paris Sant Cochin

29 rue du Faubourg Saint Jacques

75014 Paris

Phone: +33 1 82 72 61 25

www.lltechimaging.com

[email protected]

Date post:	03-Apr-2018
Category:	Documents
Upload:	lltech-management
View:	216 times
Download:	0 times

Mini Atlas March 2013b

Documents