Kinesin-5 nano-motors in cell division Kinesin-5 nano-motors in cell division
Leah GheberLeah Gheber
Departments of Clinical Biochemistry and ChemistryIlse Katz Institute for Nanoscale Science and Technology
Ben-Gurion University of the Negev, Beersheba, Israel
Mitotic spindle
human cells – specific kinesin-5 inhibitor human cells – specific kinesin-5 inhibitor monastrolmonastrol
monastrolmonastrolcontrolcontrol5 m
Leizerman et al., CMLS 2005 (Gheber lab)
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Kinesin-5
Kinesin-5 motor proteins Kinesin-5 motor proteins
Spindle assemblySpindle assembly Maintenance of bipolar Maintenance of bipolar spindle structurespindle structure
Anaphase spindle Anaphase spindle elongationelongation
Our GoalOur Goal
To understand the mechanisms and regulation To understand the mechanisms and regulation of the mitotic kinesin-5 nano motorsof the mitotic kinesin-5 nano motors
Our StrategyOur Strategy
To correlate between in vivo functions and in To correlate between in vivo functions and in vitro motor activity of specific kinesin-5 vitro motor activity of specific kinesin-5
mutants and variantsmutants and variants
In vivo assays – protein expression and In vivo assays – protein expression and quantitative live imaging quantitative live imaging
Cin8-3GFPCin8
Cin8-5A-3GFPCin8-3A
Cin8
26 37 26 37
cin8-5A
Microtubule plus-end trackingTubulinKinesin-5
In vitro - Single molecule fluorescence In vitro - Single molecule fluorescence motility assay - Kinesin-5-3GFPmotility assay - Kinesin-5-3GFP
CollaboratorsCollaboratorsChristoph Schmidt – GAUGStefan Lakamper – GAUGMart Loog – Tartu, EstoniaLiam Holt – UC Berkeley, USAndy Hoyt – JHU, US Dina Raveh – BGU, Israel
NataliaNatalia
AdinaAdina
VladimirVladimir
RachelRachel
People who do the workPeople who do the work
Vladimir FridmanVladimir Fridman
Adina Gerson-Gurwitz Natalia Adina Gerson-Gurwitz Natalia
MovshovichMovshovich
Maria PodolskayaMaria Podolskaya
Rachel Abunie-MasalaRachel Abunie-Masala
Ofer ShapiraOfer Shapira
Nurit SchkolnikNurit Schkolnik
Alina GoldsteinAlina Goldstein
AlinaAlina
OferOfer
NuritNurit
