DNA REPLICATIONLaura Restrepo Carvajal

Molecular Biology

Teacher: Lina Martínez

Medicine

UPB-Medellin

August 13/2012

In the replication one strand is synthesized

continuously and is called the leading strand,

the other is synthesizes discontinue from small

fragments called Okasaki fragments and RNA

using primers

the origin of replication site is a short repeated

sequences that is recognized by proteins

binding to sites of origin

DNA polymerases synthesize DNA only in

the direction 5 to 3, adding a dNTP to the

group 3 hydroxyl a emerging chain

Protein involved in DNA replication centrosome

regulation liked to Dwafism and small brain size

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain size

Other roles for ORC, includes centrosome

regulation and in that secuence , ORC can be

a regulator of the cromosomic division , cause

centrosome ensures that the duplicated

chromosomes are segregated evenly to the

daughter cells.

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain size

MGS patients often have

mutations in the gene encoding

the ORC therefore does not

prevent the centrosome

duplication several times.

Protein involved in DNA replication centrosome regulation linked to Dwafism and small brain size

The centrosome also plays

a role in establishing cell

outgrowths, such as axons

in brain cells and cilia in

many other cells of the

body.

Observation

Early detection of mutation of genes encoding the ORC

could give more possibilities of treatment to people

suffering from the MGS.

New England Biolabs develop novel polymerase

with ultra –high fidelity and minimal GS bias

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

High fidelity amplification is

essential for experiments whose

outcome depends upon the

correct DNA sequence, such as

sequencing, SNP detection,

protein expression and gene

function studies.

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

Q5 High-Fidelity DNA Polymerase sets a new standard for both

fidelity and robust performance. With the highest fidelity

amplification available (>50 times higher than Taq) that is frequently

used in polymerase chain reaction (PCR)

New England Biolabs develop novel polymerase with ultra –high fidelity and minimal GS bias

A hot start version of the

Q5 polymerase, which

utilizes a synthetic aptamer

for room-temperature set

up, is also available.

Observation

the uniform amplification of sequences, with robustness, coverage, high

speed , and a great amplicon length allows the efficiency of the PCR

technique

Medical Utility

Medical Utility

Survival of an organism depends

on the accurate replication of its

genome, an error in the synthesis

generates a permanent mutation

and the possible elimination of

the cell line

Medical Utility

This research has significant implications

for processes that control body and brain

size; how tissues come to be the size that

they are is a little understood area of

biology.

Studying how mutations in centrosome

biology affect tissue and body size may

lead to insights into this regulation,

Stillman suggests

Medical Utility

These new types of PCR award according to

their characteristics, greater certainty to the

diagnosis of malignant diseases such

as leukemia and lymphomas ,as will be made

much easier and faster identification of virus

or bacteria causing a disease like SIDA,

identify persons (bodies) or do scientific

research on DNA amplified

Bibliography

• MARTINEZ SANCHEZ, Lina María. Biología molecular.7.ed.Medellín: UPB.Fac.de

Medicina,2012.74-84p

• New England Biolabs Inc. (2012, 07). neb.com. Retrieved 08 09, 2012, from

http://www.neb.com:

http://www.neb.com/nebecomm/tech_reference/polymerases/Q5.asp#.UCbY0Z3v-

TI

• Biosciencetechnology. (2012, 08 1). Retrieved 08 2, 2012, from

biosciencetechnology.com: www.biosciencetechnology.com/News/2012/08/Protein-

Involved-in-DNA-Replication,-Centrosome-Regulation-Linked-to-Dwarfism,-Small-

Brain-Size/

“LIFE IS WHAT HAPPENS TO

YOU WHILE YOU'RE BUSY

MAKING OTHER PLANS." .

John Lennon