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Nardella et al. Supplementary Fig. 1 Murine Rheb 0 0.5 1 1.5 wt Line 47 Line 50 Log gene expression...

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Nardella et al. Supplementary Fig. 3 Phospho-AktPten Pten +/- PB-Rheb;Pten +/- B WTPB-RhebPten +/- PB-Rheb; Pten +/- SA  -Gal pos. cells (%) A 40X C WT PB-Rheb; Pten+/- PB-RhebPten +/- P-T389 S6K S6K

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Nardella et al. Supplementary Fig. 1 Nardella et al. Supplementary Fig. 1 Murine Rheb 0 0.5 0.5 1 1.5 1.5 wt wt Line 47 Line 47 Line 50 Line 50 Log Log gene gene expression expression (a.u.) (a.u.) * * * * * * * 200 bp Founders 47 48 49 50 51 52 59 70 86 87 88 89 90 91 A B C HA-Rheb 1 2 3 4 1- Line 50 2- WT 3- Line 47 4- pcDNA3.1 HA-Rheb transfected HeLa cells

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Page 1: Nardella et al. Supplementary Fig. 1 Murine Rheb 0 0.5 1 1.5 wt Line 47 Line 50 Log gene expression (a.u.) ******* 200 bp Founders 474849505152597086878889.

Nardella et al. Supplementary Fig. 1Nardella et al. Supplementary Fig. 1

Murine Rheb

00

0.50.5

11

1.51.5

wtwt Line 47Line 47 Line 50Line 50

Log

Log

gene

gene

exp

ress

ion

(a.u

.) e

xpre

ssio

n (a

.u.)

* * * * * * *

200 bp

Founders47 48 49 50 51 52 59 70 86 87 88 89 90 91

A

B

C

HA-Rheb

1 2 3 4

1- Line 502- WT3- Line 474- pcDNA3.1 HA-Rheb transfected HeLa cells

Page 2: Nardella et al. Supplementary Fig. 1 Murine Rheb 0 0.5 1 1.5 wt Line 47 Line 50 Log gene expression (a.u.) ******* 200 bp Founders 474849505152597086878889.

HA

-actin PIG HA-Rheb-PIG

Nardella et al. Supplementary Fig. 2

0

5

10

15

20

25

30

PIG

HA-Rheb-PIG

Rapamycin

Rheb + Vehicle Rheb + Rapamycin

-G

al +

cel

ls (%

)

+ _ _

_ + +

+__

+ _

+_

Page 3: Nardella et al. Supplementary Fig. 1 Murine Rheb 0 0.5 1 1.5 wt Line 47 Line 50 Log gene expression (a.u.) ******* 200 bp Founders 474849505152597086878889.

Nardella et al. Supplementary Fig. 3

Phospho-AktPten

Pten

+/-

PB-Rheb;Pten

+/-

B

WT PB-Rheb Pten+/- PB-Rheb;Pten+/-

SA -Gal pos. cells (%)

0

5

10

15

20

25

A

40X 40X

40X40X

C

WTPB-Rheb;Pten+/-PB-Rheb Pten+/-

P-T389 S6K

S6K

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