Nardella et al. Supplementary Fig. 1Nardella et al. Supplementary Fig. 1
Murine Rheb
00
0.50.5
11
1.51.5
wtwt Line 47Line 47 Line 50Line 50
Log
Log
gene
gene
exp
ress
ion
(a.u
.) e
xpre
ssio
n (a
.u.)
* * * * * * *
200 bp
Founders47 48 49 50 51 52 59 70 86 87 88 89 90 91
A
B
C
HA-Rheb
1 2 3 4
1- Line 502- WT3- Line 474- pcDNA3.1 HA-Rheb transfected HeLa cells
HA
-actin PIG HA-Rheb-PIG
Nardella et al. Supplementary Fig. 2
0
5
10
15
20
25
30
PIG
HA-Rheb-PIG
Rapamycin
Rheb + Vehicle Rheb + Rapamycin
-G
al +
cel
ls (%
)
+ _ _
_ + +
+__
+ _
+_
Nardella et al. Supplementary Fig. 3
Phospho-AktPten
Pten
+/-
PB-Rheb;Pten
+/-
B
WT PB-Rheb Pten+/- PB-Rheb;Pten+/-
SA -Gal pos. cells (%)
0
5
10
15
20
25
A
40X 40X
40X40X
C
WTPB-Rheb;Pten+/-PB-Rheb Pten+/-
P-T389 S6K
S6K
