NetworkAnalyst Galaxy Server Tutorial 1 –Building Collection for Paired-end RNA-seqExperiments

Choose Data Libraries under Shared Data toview our example RNA-seq files.

Click on this library for the paired-end case

1) Click on “Change” to make sure all filesare viewed for selection

2) Insert 20 for this libraryand click on “OK”.

1) Click on this checkbox to select allfiles.

2) After file selection, click on “To History” andchoose “as a Collection”.

1) Select “List of Pairs” forpaired-end experiments

2) Click on “Continue”

2) Assign a name to thecollection

1) Galaxy provides aflexible way to pairsamples using a filterwith certain pattern.For example, “_1”refers to forward readsand “_2” for thereverse ones.

3) Finally, click on“Create list” and nowyou can run paired-endworkflows. They willdirectly recognize thecreated collection aslong as you run fromthe same history.

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