Page 1
ONLINE SUPPLEMENT FOR THE PAPER
STEREOCONTROL OF ARACHIDONIC ACID OXYGENATION BY VERTEBRATE LIPOXYGENASES. NEWLY CLONED ZEBRAFISH LIPOXYGENASE1 DOES NOT FOLLOW
THE A-vs-G CONCEPT. Christian Jansen*, Katharina Hofheinz*, Robert Vogel*, Jana Roffeis*, Monika Anton*, Pallu Reddanna$, Hartmut Kuhn*+, Matthias Walther* *Institute of Biochemistry, University Medicine Berlin - Charité, Monbijoustr. 2, D-10117 Berlin, F.R. Germany; $School of Life Sciences, University of Hyderabad, Hyderabad-500046, Andhra Pradesh, India; Supplemental Fig. 1: Zebrafisch LOX sequences and sequence alignments with selected mammalian LOX isoforms ENSDARP00000091900 Chromosom 7, according to sequence homology a 12/15-LOX or
platelet-type 12-LOX, referred to as zebrafish LOX1 in this paper. ENSDARP00000074335 Chromosome 13 (highest homology to human 5-LOX), referred to as
zebrafish LOX2 in this paper. ENSDARP00000063256 Chromosom 15, according to sequence homology an alternative 5-
LOX transcript. ENSDARP00000093033 Chromosom 15, according to sequence homology an alternative 5-
LOX transcript. ENSDARP00000117752 Chromosom 15, according to sequence homology an alternative 5-
LOX transcript. ENSDARP00000063263 Chromosom 15, 99% sequence identity with ENSDARP00000117752,
according to sequence homology an alternative 5-LOX transcript. ENSDARP00000106795 Chromosome 21, long transcript (possibly an epidermal LOX
isoform). ENSDARP00000023401 Chromosom 21, short transcript (possibly an epidermal LOX isoform,
100% sequence identity to ENSDARP00000106795. ENSDARP00000113298 Chromosome 21, possibly an epidermal LOX isoform (15-LOX2),
short transcript. ENSDARP00000095620 Chromosome 21, possibly an epidermal LOX isoform (15-LOX2),
long transcript, 99% sequence identity with ENSDARP00000113298.
Page 2
SeqA Name Length SeqB Name Length Score
1 ENSDARP00000074335 674 2 ENSDARP00000023401 663 36.0
1 ENSDARP00000074335 674 3 ENSDARP00000091900 670 46.0
1 ENSDARP00000074335 674 4 ENSDARP00000063256 676 63.0
1 ENSDARP00000074335 674 5 ENSDARP00000093033 676 63.0
1 ENSDARP00000074335 674 6 ENSDARP00000063263 676 64.0
1 ENSDARP00000074335 674 7 ENSDARP00000113298 669 38.0
1 ENSDARP00000074335 674 8 bovine_12/15-LOX 663 38.0
1 ENSDARP00000074335 674 9 human_12/15-LOX 662 37.0
1 ENSDARP00000074335 674 10 rabbit_12/15-LOX 663 36.0
1 ENSDARP00000074335 674 11 porcine_12/15-LOX 663 38.0
1 ENSDARP00000074335 674 12 rat_12/15-LOX 663 37.0
1 ENSDARP00000074335 674 13 mouse_12/15-LOX 663 37.0
1 ENSDARP00000074335 674 14 mouse_e12-LOX 662 36.0
1 ENSDARP00000074335 674 15 mouse_p12-LOX 663 38.0
1 ENSDARP00000074335 674 16 human_p12-LOX 663 38.0
1 ENSDARP00000074335 674 17 human_15-LOX2 676 39.0
1 ENSDARP00000074335 674 18 mouse_8-LOX 677 41.0
1 ENSDARP00000074335 674 19 human_12R-LOX 701 40.0
1 ENSDARP00000074335 674 20 mouse_12R-LOX 701 39.0
1 ENSDARP00000074335 674 21 mouse_5-LOX 674 73.0
1 ENSDARP00000074335 674 22 rat_5-LOX 673 73.0
1 ENSDARP00000074335 674 23 guinea_Pig_5-LOX 673 73.0
1 ENSDARP00000074335 674 24 human_5-LOX 674 74.0
1 ENSDARP00000074335 674 25 human_eLOX3 843 41.0
1 ENSDARP00000074335 674 26 mouse_eLOX3 711 42.0
2 ENSDARP00000023401 663 3 ENSDARP00000091900 670 36.0
2 ENSDARP00000023401 663 4 ENSDARP00000063256 676 36.0
2 ENSDARP00000023401 663 5 ENSDARP00000093033 676 35.0
2 ENSDARP00000023401 663 6 ENSDARP00000063263 676 36.0
2 ENSDARP00000023401 663 7 ENSDARP00000113298 669 38.0
2 ENSDARP00000023401 663 8 bovine_12/15-LOX 663 35.0
2 ENSDARP00000023401 663 9 human_12/15-LOX 662 34.0
2 ENSDARP00000023401 663 10 rabbit_12/15-LOX 663 34.0
2 ENSDARP00000023401 663 11 porcine_12/15-LOX 663 34.0
2 ENSDARP00000023401 663 12 rat_12/15-LOX 663 35.0
2 ENSDARP00000023401 663 13 mouse_12/15-LOX 663 35.0
2 ENSDARP00000023401 663 14 mouse_e12-LOX 662 34.0
2 ENSDARP00000023401 663 15 mouse_p12-LOX 663 37.0
2 ENSDARP00000023401 663 16 human_p12-LOX 663 37.0
2 ENSDARP00000023401 663 17 human_15-LOX2 676 39.0
2 ENSDARP00000023401 663 18 mouse_8-LOX 677 40.0
2 ENSDARP00000023401 663 19 human_12R-LOX 701 37.0
2 ENSDARP00000023401 663 20 mouse_12R-LOX 701 36.0
Page 3
SeqA Name Length SeqB Name Length Score
2 ENSDARP00000023401 663 21 mouse_5-LOX 674 38.0
2 ENSDARP00000023401 663 22 rat_5-LOX 673 38.0
2 ENSDARP00000023401 663 23 guinea_Pig_5-LOX 673 38.0
2 ENSDARP00000023401 663 24 human_5-LOX 674 37.0
2 ENSDARP00000023401 663 25 human_eLOX3 843 38.0
2 ENSDARP00000023401 663 26 mouse_eLOX3 711 38.0
3 ENSDARP00000091900 670 4 ENSDARP00000063256 676 44.0
3 ENSDARP00000091900 670 5 ENSDARP00000093033 676 44.0
3 ENSDARP00000091900 670 6 ENSDARP00000063263 676 43.0
3 ENSDARP00000091900 670 7 ENSDARP00000113298 669 40.0
3 ENSDARP00000091900 670 8 bovine_12/15-LOX 663 45.0
3 ENSDARP00000091900 670 9 human_12/15-LOX 662 44.0
3 ENSDARP00000091900 670 10 rabbit_12/15-LOX 663 44.0
3 ENSDARP00000091900 670 11 porcine_12/15-LOX 663 45.0
3 ENSDARP00000091900 670 12 rat_12/15-LOX 663 42.0
3 ENSDARP00000091900 670 13 mouse_12/15-LOX 663 43.0
3 ENSDARP00000091900 670 14 mouse_e12-LOX 662 42.0
3 ENSDARP00000091900 670 15 mouse_p12-LOX 663 46.0
3 ENSDARP00000091900 670 16 human_p12-LOX 663 45.0
3 ENSDARP00000091900 670 17 human_15-LOX2 676 43.0
3 ENSDARP00000091900 670 18 mouse_8-LOX 677 41.0
3 ENSDARP00000091900 670 19 human_12R-LOX 701 41.0
3 ENSDARP00000091900 670 20 mouse_12R-LOX 701 40.0
3 ENSDARP00000091900 670 21 mouse_5-LOX 674 44.0
3 ENSDARP00000091900 670 22 rat_5-LOX 673 44.0
3 ENSDARP00000091900 670 23 guinea_Pig_5-LOX 673 44.0
3 ENSDARP00000091900 670 24 human_5-LOX 674 45.0
3 ENSDARP00000091900 670 25 human_eLOX3 843 41.0
3 ENSDARP00000091900 670 26 mouse_eLOX3 711 41.0
4 ENSDARP00000063256 676 5 ENSDARP00000093033 676 84.0
4 ENSDARP00000063256 676 6 ENSDARP00000063263 676 84.0
4 ENSDARP00000063256 676 7 ENSDARP00000113298 669 37.0
4 ENSDARP00000063256 676 8 bovine_12/15-LOX 663 38.0
4 ENSDARP00000063256 676 9 human_12/15-LOX 662 37.0
4 ENSDARP00000063256 676 10 rabbit_12/15-LOX 663 36.0
4 ENSDARP00000063256 676 11 porcine_12/15-LOX 663 38.0
4 ENSDARP00000063256 676 12 rat_12/15-LOX 663 37.0
4 ENSDARP00000063256 676 13 mouse_12/15-LOX 663 37.0
4 ENSDARP00000063256 676 14 mouse_e12-LOX 662 36.0
4 ENSDARP00000063256 676 15 mouse_p12-LOX 663 39.0
4 ENSDARP00000063256 676 16 human_p12-LOX 663 38.0
4 ENSDARP00000063256 676 17 human_15-LOX2 676 39.0
4 ENSDARP00000063256 676 18 mouse_8-LOX 677 39.0
Page 4
SeqA Name Length SeqB Name Length Score
4 ENSDARP00000063256 676 19 human_12R-LOX 701 39.0
4 ENSDARP00000063256 676 20 mouse_12R-LOX 701 39.0
4 ENSDARP00000063256 676 21 mouse_5-LOX 674 58.0
4 ENSDARP00000063256 676 22 rat_5-LOX 673 57.0
4 ENSDARP00000063256 676 23 guinea_Pig_5-LOX 673 57.0
4 ENSDARP00000063256 676 24 human_5-LOX 674 58.0
4 ENSDARP00000063256 676 25 human_eLOX3 843 40.0
4 ENSDARP00000063256 676 26 mouse_eLOX3 711 41.0
5 ENSDARP00000093033 676 6 ENSDARP00000063263 676 82.0
5 ENSDARP00000093033 676 7 ENSDARP00000113298 669 36.0
5 ENSDARP00000093033 676 8 bovine_12/15-LOX 663 38.0
5 ENSDARP00000093033 676 9 human_12/15-LOX 662 37.0
5 ENSDARP00000093033 676 10 rabbit_12/15-LOX 663 36.0
5 ENSDARP00000093033 676 11 porcine_12/15-LOX 663 38.0
5 ENSDARP00000093033 676 12 rat_12/15-LOX 663 37.0
5 ENSDARP00000093033 676 13 mouse_12/15-LOX 663 37.0
5 ENSDARP00000093033 676 14 mouse_e12-LOX 662 37.0
5 ENSDARP00000093033 676 15 mouse_p12-LOX 663 39.0
5 ENSDARP00000093033 676 16 human_p12-LOX 663 38.0
5 ENSDARP00000093033 676 17 human_15-LOX2 676 39.0
5 ENSDARP00000093033 676 18 mouse_8-LOX 677 39.0
5 ENSDARP00000093033 676 19 human_12R-LOX 701 40.0
5 ENSDARP00000093033 676 20 mouse_12R-LOX 701 40.0
5 ENSDARP00000093033 676 21 mouse_5-LOX 674 58.0
5 ENSDARP00000093033 676 22 rat_5-LOX 673 58.0
5 ENSDARP00000093033 676 23 guinea_Pig_5-LOX 673 58.0
5 ENSDARP00000093033 676 24 human_5-LOX 674 59.0
5 ENSDARP00000093033 676 25 human_eLOX3 843 41.0
5 ENSDARP00000093033 676 26 mouse_eLOX3 711 42.0
6 ENSDARP00000063263 676 7 ENSDARP00000113298 669 37.0
6 ENSDARP00000063263 676 8 bovine_12/15-LOX 663 37.0
6 ENSDARP00000063263 676 9 human_12/15-LOX 662 36.0
6 ENSDARP00000063263 676 10 rabbit_12/15-LOX 663 35.0
6 ENSDARP00000063263 676 11 porcine_12/15-LOX 663 37.0
6 ENSDARP00000063263 676 12 rat_12/15-LOX 663 36.0
6 ENSDARP00000063263 676 13 mouse_12/15-LOX 663 37.0
6 ENSDARP00000063263 676 14 mouse_e12-LOX 662 37.0
6 ENSDARP00000063263 676 15 mouse_p12-LOX 663 38.0
6 ENSDARP00000063263 676 16 human_p12-LOX 663 38.0
6 ENSDARP00000063263 676 17 human_15-LOX2 676 39.0
6 ENSDARP00000063263 676 18 mouse_8-LOX 677 39.0
6 ENSDARP00000063263 676 19 human_12R-LOX 701 39.0
6 ENSDARP00000063263 676 20 mouse_12R-LOX 701 39.0
Page 5
SeqA Name Length SeqB Name Length Score
6 ENSDARP00000063263 676 21 mouse_5-LOX 674 57.0
6 ENSDARP00000063263 676 22 rat_5-LOX 673 57.0
6 ENSDARP00000063263 676 23 guinea_Pig_5-LOX 673 57.0
6 ENSDARP00000063263 676 24 human_5-LOX 674 57.0
6 ENSDARP00000063263 676 25 human_eLOX3 843 41.0
6 ENSDARP00000063263 676 26 mouse_eLOX3 711 42.0
7 ENSDARP00000113298 669 8 bovine_12/15-LOX 663 34.0
7 ENSDARP00000113298 669 9 human_12/15-LOX 662 33.0
7 ENSDARP00000113298 669 10 rabbit_12/15-LOX 663 34.0
7 ENSDARP00000113298 669 11 porcine_12/15-LOX 663 34.0
7 ENSDARP00000113298 669 12 rat_12/15-LOX 663 34.0
7 ENSDARP00000113298 669 13 mouse_12/15-LOX 663 33.0
7 ENSDARP00000113298 669 14 mouse_e12-LOX 662 34.0
7 ENSDARP00000113298 669 15 mouse_p12-LOX 663 34.0
7 ENSDARP00000113298 669 16 human_p12-LOX 663 34.0
7 ENSDARP00000113298 669 17 human_15-LOX2 676 40.0
7 ENSDARP00000113298 669 18 mouse_8-LOX 677 38.0
7 ENSDARP00000113298 669 19 human_12R-LOX 701 40.0
7 ENSDARP00000113298 669 20 mouse_12R-LOX 701 40.0
7 ENSDARP00000113298 669 21 mouse_5-LOX 674 38.0
7 ENSDARP00000113298 669 22 rat_5-LOX 673 38.0
7 ENSDARP00000113298 669 23 guinea_Pig_5-LOX 673 39.0
7 ENSDARP00000113298 669 24 human_5-LOX 674 38.0
7 ENSDARP00000113298 669 25 human_eLOX3 843 44.0
7 ENSDARP00000113298 669 26 mouse_eLOX3 711 44.0
8 bovine_12/15-LOX 663 9 human_12/15-LOX 662 86.0
8 bovine_12/15-LOX 663 10 rabbit_12/15-LOX 663 78.0
8 bovine_12/15-LOX 663 11 porcine_12/15-LOX 663 88.0
8 bovine_12/15-LOX 663 12 rat_12/15-LOX 663 72.0
8 bovine_12/15-LOX 663 13 mouse_12/15-LOX 663 72.0
8 bovine_12/15-LOX 663 14 mouse_e12-LOX 662 66.0
8 bovine_12/15-LOX 663 15 mouse_p12-LOX 663 63.0
8 bovine_12/15-LOX 663 16 human_p12-LOX 663 64.0
8 bovine_12/15-LOX 663 17 human_15-LOX2 676 38.0
8 bovine_12/15-LOX 663 18 mouse_8-LOX 677 37.0
8 bovine_12/15-LOX 663 19 human_12R-LOX 701 35.0
8 bovine_12/15-LOX 663 20 mouse_12R-LOX 701 34.0
8 bovine_12/15-LOX 663 21 mouse_5-LOX 674 40.0
8 bovine_12/15-LOX 663 22 rat_5-LOX 673 40.0
8 bovine_12/15-LOX 663 23 guinea_Pig_5-LOX 673 39.0
8 bovine_12/15-LOX 663 24 human_5-LOX 674 40.0
8 bovine_12/15-LOX 663 25 human_eLOX3 843 37.0
8 bovine_12/15-LOX 663 26 mouse_eLOX3 711 37.0
Page 6
SeqA Name Length SeqB Name Length Score
9 human_12/15-LOX 662 10 rabbit_12/15-LOX 663 81.0
9 human_12/15-LOX 662 11 porcine_12/15-LOX 663 86.0
9 human_12/15-LOX 662 12 rat_12/15-LOX 663 74.0
9 human_12/15-LOX 662 13 mouse_12/15-LOX 663 73.0
9 human_12/15-LOX 662 14 mouse_e12-LOX 662 66.0
9 human_12/15-LOX 662 15 mouse_p12-LOX 663 63.0
9 human_12/15-LOX 662 16 human_p12-LOX 663 65.0
9 human_12/15-LOX 662 17 human_15-LOX2 676 37.0
9 human_12/15-LOX 662 18 mouse_8-LOX 677 37.0
9 human_12/15-LOX 662 19 human_12R-LOX 701 35.0
9 human_12/15-LOX 662 20 mouse_12R-LOX 701 34.0
9 human_12/15-LOX 662 21 mouse_5-LOX 674 40.0
9 human_12/15-LOX 662 22 rat_5-LOX 673 40.0
9 human_12/15-LOX 662 23 guinea_Pig_5-LOX 673 39.0
9 human_12/15-LOX 662 24 human_5-LOX 674 40.0
9 human_12/15-LOX 662 25 human_eLOX3 843 36.0
9 human_12/15-LOX 662 26 mouse_eLOX3 711 37.0
10 rabbit_12/15-LOX 663 11 porcine_12/15-LOX 663 79.0
10 rabbit_12/15-LOX 663 12 rat_12/15-LOX 663 71.0
10 rabbit_12/15-LOX 663 13 mouse_12/15-LOX 663 71.0
10 rabbit_12/15-LOX 663 14 mouse_e12-LOX 662 62.0
10 rabbit_12/15-LOX 663 15 mouse_p12-LOX 663 60.0
10 rabbit_12/15-LOX 663 16 human_p12-LOX 663 61.0
10 rabbit_12/15-LOX 663 17 human_15-LOX2 676 36.0
10 rabbit_12/15-LOX 663 18 mouse_8-LOX 677 36.0
10 rabbit_12/15-LOX 663 19 human_12R-LOX 701 35.0
10 rabbit_12/15-LOX 663 20 mouse_12R-LOX 701 35.0
10 rabbit_12/15-LOX 663 21 mouse_5-LOX 674 38.0
10 rabbit_12/15-LOX 663 22 rat_5-LOX 673 38.0
10 rabbit_12/15-LOX 663 23 guinea_Pig_5-LOX 673 38.0
10 rabbit_12/15-LOX 663 24 human_5-LOX 674 39.0
10 rabbit_12/15-LOX 663 25 human_eLOX3 843 37.0
10 rabbit_12/15-LOX 663 26 mouse_eLOX3 711 37.0
11 porcine_12/15-LOX 663 12 rat_12/15-LOX 663 71.0
11 porcine_12/15-LOX 663 13 mouse_12/15-LOX 663 70.0
11 porcine_12/15-LOX 663 14 mouse_e12-LOX 662 65.0
11 porcine_12/15-LOX 663 15 mouse_p12-LOX 663 64.0
11 porcine_12/15-LOX 663 16 human_p12-LOX 663 65.0
11 porcine_12/15-LOX 663 17 human_15-LOX2 676 37.0
11 porcine_12/15-LOX 663 18 mouse_8-LOX 677 37.0
11 porcine_12/15-LOX 663 19 human_12R-LOX 701 34.0
11 porcine_12/15-LOX 663 20 mouse_12R-LOX 701 34.0
11 porcine_12/15-LOX 663 21 mouse_5-LOX 674 40.0
Page 7
SeqA Name Length SeqB Name Length Score
11 porcine_12/15-LOX 663 22 rat_5-LOX 673 40.0
11 porcine_12/15-LOX 663 23 guinea_Pig_5-LOX 673 40.0
11 porcine_12/15-LOX 663 24 human_5-LOX 674 41.0
11 porcine_12/15-LOX 663 25 human_eLOX3 843 37.0
11 porcine_12/15-LOX 663 26 mouse_eLOX3 711 37.0
12 rat_12/15-LOX 663 13 mouse_12/15-LOX 663 93.0
12 rat_12/15-LOX 663 14 mouse_e12-LOX 662 59.0
12 rat_12/15-LOX 663 15 mouse_p12-LOX 663 57.0
12 rat_12/15-LOX 663 16 human_p12-LOX 663 58.0
12 rat_12/15-LOX 663 17 human_15-LOX2 676 36.0
12 rat_12/15-LOX 663 18 mouse_8-LOX 677 37.0
12 rat_12/15-LOX 663 19 human_12R-LOX 701 35.0
12 rat_12/15-LOX 663 20 mouse_12R-LOX 701 35.0
12 rat_12/15-LOX 663 21 mouse_5-LOX 674 39.0
12 rat_12/15-LOX 663 22 rat_5-LOX 673 39.0
12 rat_12/15-LOX 663 23 guinea_Pig_5-LOX 673 39.0
12 rat_12/15-LOX 663 24 human_5-LOX 674 40.0
12 rat_12/15-LOX 663 25 human_eLOX3 843 37.0
12 rat_12/15-LOX 663 26 mouse_eLOX3 711 38.0
13 mouse_12/15-LOX 663 14 mouse_e12-LOX 662 59.0
13 mouse_12/15-LOX 663 15 mouse_p12-LOX 663 58.0
13 mouse_12/15-LOX 663 16 human_p12-LOX 663 57.0
13 mouse_12/15-LOX 663 17 human_15-LOX2 676 37.0
13 mouse_12/15-LOX 663 18 mouse_8-LOX 677 37.0
13 mouse_12/15-LOX 663 19 human_12R-LOX 701 35.0
13 mouse_12/15-LOX 663 20 mouse_12R-LOX 701 35.0
13 mouse_12/15-LOX 663 21 mouse_5-LOX 674 40.0
13 mouse_12/15-LOX 663 22 rat_5-LOX 673 40.0
13 mouse_12/15-LOX 663 23 guinea_Pig_5-LOX 673 40.0
13 mouse_12/15-LOX 663 24 human_5-LOX 674 40.0
13 mouse_12/15-LOX 663 25 human_eLOX3 843 37.0
13 mouse_12/15-LOX 663 26 mouse_eLOX3 711 38.0
14 mouse_e12-LOX 662 15 mouse_p12-LOX 663 59.0
14 mouse_e12-LOX 662 16 human_p12-LOX 663 59.0
14 mouse_e12-LOX 662 17 human_15-LOX2 676 36.0
14 mouse_e12-LOX 662 18 mouse_8-LOX 677 36.0
14 mouse_e12-LOX 662 19 human_12R-LOX 701 35.0
14 mouse_e12-LOX 662 20 mouse_12R-LOX 701 34.0
14 mouse_e12-LOX 662 21 mouse_5-LOX 674 38.0
14 mouse_e12-LOX 662 22 rat_5-LOX 673 38.0
14 mouse_e12-LOX 662 23 guinea_Pig_5-LOX 673 38.0
14 mouse_e12-LOX 662 24 human_5-LOX 674 39.0
14 mouse_e12-LOX 662 25 human_eLOX3 843 35.0
Page 8
SeqA Name Length SeqB Name Length Score
14 mouse_e12-LOX 662 26 mouse_eLOX3 711 35.0
15 mouse_p12-LOX 663 16 human_p12-LOX 663 85.0
15 mouse_p12-LOX 663 17 human_15-LOX2 676 38.0
15 mouse_p12-LOX 663 18 mouse_8-LOX 677 38.0
15 mouse_p12-LOX 663 19 human_12R-LOX 701 36.0
15 mouse_p12-LOX 663 20 mouse_12R-LOX 701 36.0
15 mouse_p12-LOX 663 21 mouse_5-LOX 674 40.0
15 mouse_p12-LOX 663 22 rat_5-LOX 673 40.0
15 mouse_p12-LOX 663 23 guinea_Pig_5-LOX 673 40.0
15 mouse_p12-LOX 663 24 human_5-LOX 674 41.0
15 mouse_p12-LOX 663 25 human_eLOX3 843 37.0
15 mouse_p12-LOX 663 26 mouse_eLOX3 711 37.0
16 human_p12-LOX 663 17 human_15-LOX2 676 37.0
16 human_p12-LOX 663 18 mouse_8-LOX 677 37.0
16 human_p12-LOX 663 19 human_12R-LOX 701 38.0
16 human_p12-LOX 663 20 mouse_12R-LOX 701 37.0
16 human_p12-LOX 663 21 mouse_5-LOX 674 40.0
16 human_p12-LOX 663 22 rat_5-LOX 673 40.0
16 human_p12-LOX 663 23 guinea_Pig_5-LOX 673 40.0
16 human_p12-LOX 663 24 human_5-LOX 674 42.0
16 human_p12-LOX 663 25 human_eLOX3 843 37.0
16 human_p12-LOX 663 26 mouse_eLOX3 711 38.0
17 human_15-LOX2 676 18 mouse_8-LOX 677 78.0
17 human_15-LOX2 676 19 human_12R-LOX 701 50.0
17 human_15-LOX2 676 20 mouse_12R-LOX 701 50.0
17 human_15-LOX2 676 21 mouse_5-LOX 674 41.0
17 human_15-LOX2 676 22 rat_5-LOX 673 41.0
17 human_15-LOX2 676 23 guinea_Pig_5-LOX 673 41.0
17 human_15-LOX2 676 24 human_5-LOX 674 41.0
17 human_15-LOX2 676 25 human_eLOX3 843 54.0
17 human_15-LOX2 676 26 mouse_eLOX3 711 54.0
18 mouse_8-LOX 677 19 human_12R-LOX 701 50.0
18 mouse_8-LOX 677 20 mouse_12R-LOX 701 48.0
18 mouse_8-LOX 677 21 mouse_5-LOX 674 42.0
18 mouse_8-LOX 677 22 rat_5-LOX 673 42.0
18 mouse_8-LOX 677 23 guinea_Pig_5-LOX 673 42.0
18 mouse_8-LOX 677 24 human_5-LOX 674 42.0
18 mouse_8-LOX 677 25 human_eLOX3 843 53.0
18 mouse_8-LOX 677 26 mouse_eLOX3 711 53.0
19 human_12R-LOX 701 20 mouse_12R-LOX 701 86.0
19 human_12R-LOX 701 21 mouse_5-LOX 674 41.0
19 human_12R-LOX 701 22 rat_5-LOX 673 41.0
19 human_12R-LOX 701 23 guinea_Pig_5-LOX 673 40.0
Page 9
SeqA Name Length SeqB Name Length Score
19 human_12R-LOX 701 24 human_5-LOX 674 41.0
19 human_12R-LOX 701 25 human_eLOX3 843 54.0
19 human_12R-LOX 701 26 mouse_eLOX3 711 55.0
20 mouse_12R-LOX 701 21 mouse_5-LOX 674 40.0
20 mouse_12R-LOX 701 22 rat_5-LOX 673 40.0
20 mouse_12R-LOX 701 23 guinea_Pig_5-LOX 673 39.0
20 mouse_12R-LOX 701 24 human_5-LOX 674 40.0
20 mouse_12R-LOX 701 25 human_eLOX3 843 53.0
20 mouse_12R-LOX 701 26 mouse_eLOX3 711 54.0
21 mouse_5-LOX 674 22 rat_5-LOX 673 97.0
21 mouse_5-LOX 674 23 guinea_Pig_5-LOX 673 96.0
21 mouse_5-LOX 674 24 human_5-LOX 674 93.0
21 mouse_5-LOX 674 25 human_eLOX3 843 40.0
21 mouse_5-LOX 674 26 mouse_eLOX3 711 41.0
22 rat_5-LOX 673 23 guinea_Pig_5-LOX 673 96.0
22 rat_5-LOX 673 24 human_5-LOX 674 93.0
22 rat_5-LOX 673 25 human_eLOX3 843 40.0
22 rat_5-LOX 673 26 mouse_eLOX3 711 41.0
23 guinea_Pig_5-LOX 673 24 human_5-LOX 674 93.0
23 guinea_Pig_5-LOX 673 25 human_eLOX3 843 40.0
23 guinea_Pig_5-LOX 673 26 mouse_eLOX3 711 41.0
24 human_5-LOX 674 25 human_eLOX3 843 41.0
24 human_5-LOX 674 26 mouse_eLOX3 711 42.0
25 human_eLOX3 843 26 mouse_eLOX3 711 88.0 CLUSTAL 2.1 multiple sequence alignment mouse_5-LOX ------------------------------------------------------------ rat_5-LOX ------------------------------------------------------------ guinea_Pig_5-LOX ------------------------------------------------------------ human_5-LOX ------------------------------------------------------------ ENSDARP00000074335 ------------------------------------------------------------ ENSDARP00000063256 ------------------------------------------------------------ ENSDARP00000063263 ------------------------------------------------------------ ENSDARP00000093033 ------------------------------------------------------------ human_15-LOX2 ------------------------------------------------------------ mouse_8-LOX ------------------------------------------------------------ human_12R-LOX ------------------------------------------------------------ mouse_12R-LOX ------------------------------------------------------------ human_eLOX3 MPRGAFRPCLPALYFAFLTCPTPEQRMSGTQAPDIHLGEPARGTGCVRGKQTSIRVQDCG 60 mouse_eLOX3 ------------------------------------------------------------ ENSDARP00000023401 ------------------------------------------------------------ ENSDARP00000113298 ------------------------------------------------------------ bovine_12/15-LOX ------------------------------------------------------------ porcine_12/15-LOX ------------------------------------------------------------ human_12/15-LOX ------------------------------------------------------------ rabbit_12/15-LOX ------------------------------------------------------------ rat_12/15-LOX ------------------------------------------------------------ mouse_12/15-LOX ------------------------------------------------------------ mouse_e12-LOX ------------------------------------------------------------ mouse_p12-LOX ------------------------------------------------------------ human_p12-LOX ------------------------------------------------------------ ENSDARP00000091900 ------------------------------------------------------------
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mouse_5-LOX ------------------------------------------------------------ rat_5-LOX ------------------------------------------------------------ guinea_Pig_5-LOX ------------------------------------------------------------ human_5-LOX ------------------------------------------------------------ ENSDARP00000074335 ------------------------------------------------------------ ENSDARP00000063256 ------------------------------------------------------------ ENSDARP00000063263 ------------------------------------------------------------ ENSDARP00000093033 ------------------------------------------------------------ human_15-LOX2 ------------------------------------------------------------ mouse_8-LOX ------------------------------------------------------------ human_12R-LOX ------------------------------------------------------------ mouse_12R-LOX ------------------------------------------------------------ human_eLOX3 RREEARAASRELRREKAQEHPRESWAHPQPYPAPQPLALRPETQPCPACRSSPPGRLLLR 120 mouse_eLOX3 ------------------------------------------------------------ ENSDARP00000023401 ------------------------------------------------------------ ENSDARP00000113298 ------------------------------------------------------------ bovine_12/15-LOX ------------------------------------------------------------ porcine_12/15-LOX ------------------------------------------------------------ human_12/15-LOX ------------------------------------------------------------ rabbit_12/15-LOX ------------------------------------------------------------ rat_12/15-LOX ------------------------------------------------------------ mouse_12/15-LOX ------------------------------------------------------------ mouse_e12-LOX ------------------------------------------------------------ mouse_p12-LOX ------------------------------------------------------------ human_p12-LOX ------------------------------------------------------------ ENSDARP00000091900 ------------------------------------------------------------ mouse_5-LOX ------------MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFER 48 rat_5-LOX ------------MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFER 48 guinea_Pig_5-LOX ------------MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFER 48 human_5-LOX ------------MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFER 48 ENSDARP00000074335 ------------MPSYTVTVATGTQWFAGTDDYIYLTLVGSEGCSDRTLLDKPLYNDFER 48 ENSDARP00000063256 ------------MFTYTVSVVTGNQVFAGTLNYIYLTLVGEERSSDRTLLDKSWFERLDR 48 ENSDARP00000063263 ------------MFTYTVSVTTGNQVFAGTLNYIYLTLVGKERSSDRTLLDKSWFERLDR 48 ENSDARP00000093033 ------------MFTYSVSVETGDQAFAGTLNYIYLTLVGDKRSSVRTLLDKSWLKRLER 48 human_15-LOX2 ------------MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGK-EFTA 47 mouse_8-LOX ------------MAKCRVRVSTGEACGAGTWDKVSVSIVGTHGESPLVPLDHLGK-EFSA 47 human_12R-LOX ------------MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGR-DFAT 47 mouse_12R-LOX ------------MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGR-DFAT 47 human_eLOX3 PALPGHPFLLPIMAVYRLCVTTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGR-DFAP 179 mouse_eLOX3 ------------MAVYRLCVTTGSYLKAGTLDNIYATLVGTCGESPKQKLDRVGR-DFAS 47 ENSDARP00000023401 ------------MGGFHVTVRTSAFPVSGTYSSVWISLIGSLCESPPIKVEHR----LLP 44 ENSDARP00000113298 -----------MDAVYEVEVTTGSMAHAGTFDNIFITLIGIEGESERNKLDTYGR-DFNI 48 bovine_12/15-LOX ------------MGLYRVRVSTGSSFCAGSNNQVHLWLVGEHGEAALGWAVRP-----AR 43 porcine_12/15-LOX ------------MGLYRVRVSTGSSFYAGSQNQVQLWLVGQHGEAALGWCLRP-----AR 43 human_12/15-LOX ------------MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGKRLWP-----AR 43 rabbit_12/15-LOX ------------MGVYRVCVSTGASIYAGSKNKVELWLVGQHGEVELGSCLRP-----TR 43 rat_12/15-LOX ------------MGVYRIRVSTGDSKYAGSNNEVYLWLVGQHGEASLGKLLRP-----CR 43 mouse_12/15-LOX ------------MGVYRIRVSTGDSVYAGSNNEVYLWLIGQHGEASLGKLFRP-----CR 43 mouse_e12-LOX ------------MVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRP-----LL 43 mouse_p12-LOX ------------MGAYRVRVVTGAWLFSGSLNLVRLWLVGEHREAKLELQLRP-----AR 43 human_p12-LOX ------------MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRP-----AR 43 ENSDARP00000091900 -------------MEYKVTVATGTSEYSGTNNYVYVTLIGEKGKSERTLLDNPGL-DFCR 46 : * *. :*: . : ::* mouse_5-LOX GAVDSYDVTVDEELGEIYLVKIEKRK--------YWLHDDWYLKYITLKTPHGD--YIEF 98 rat_5-LOX GGRDSYDVTVDEELGEIYLVKIEKRK--------YRLHDDWYLKYITLKTPH-D--YIEF 97 guinea_Pig_5-LOX GAVDSYDVTVDEELGEIQLVRIEKRK--------YWLHDDWYLKYITLKTPT-D--YIEF 97 human_5-LOX GAVDSYDVTVDEELGEIQLVRIEKRK--------YWLNDDWYLKYITLKTPHGD--YIEF 98 ENSDARP00000074335 GAVDSYDISVGEDLGEIQLVKIEKKK--------YWMHDDWYCKYITVKTPYGD--YVEF 98 ENSDARP00000063256 GKVVSIDIHVEETLGELLLVKLEKEK--------FLINDDWYCRSISVTAPAGD--CFEF 98 ENSDARP00000063263 GTVVSIDIHVEETLGELLLVKLEKEK--------FLINDDWYCRSISVTAPTGD--CFEF 98 ENSDARP00000093033 GTVISIDVHVEETLGELLLVKLEKEK--------TLINDQWYCRSISVTAPTGD--CFEF 98 human_15-LOX2 GAEEDFQVTLPEDVGRVLLLRVHKAPPVLPL-LGPLAPDAWFCRWFQLTPPRGG--HLLF 104 mouse_8-LOX GAEEDFEVTLPQDVGTVLMLRVHKAPPEVSLPLMSFRSDAWFCRWFELEWLPGA--ALHF 105 human_12R-LOX GAVGQYTVQCPQDLGELIIIRLHKER------YAFFPKDPWYCNYVQICAPNGR--IYHF 99 mouse_12R-LOX GAVDDYTVQCQQDLGELIIIRLHKEP------HSFLAKDPWYCNYVQICAPDCR--VYHF 99 human_eLOX3 GSVQKYKVRCTAELGELLLLRVHKER------YAFFRKDSWYCSRICVTEPDGS--VSHF 231 mouse_eLOX3 GSVQKYKVRCEAELGEILLLRLHKER------FAFFCKDPWYCSRICVTAPDGS--AVHF 99 ENSDARP00000023401 GSACTVVIMPEEEVGILVLVKLRLEA------KPGFPDLDWHCQDVQVKQSSEDPEMEVF 98 ENSDARP00000113298 GMKVTYKVTTRLSLGTLLLVRLEKDN------FLFLPENDWFCSLVNIKTPEKD--VISF 100 bovine_12/15-LOX GKEVEFQVDVSEYLGRLLFVKLRKRH--------LLSDDAWFCNWISVQGPGASGNEFRF 95 porcine_12/15-LOX GKETEFSVDVSEYLGPLLFVKLRKRH--------LLQDDAWFCNWISVQGPGANGDEFRF 95 human_12/15-LOX GKETELKVEVPEYLGPLLFVKLRKRH--------LLKDDAWFCNWISVQGPGA-GDEVRF 94 rabbit_12/15-LOX NKEEEFKVNVSKYLGSLLFVRLRKKH--------FLKEDAWFCNWISVQALGAAEDKYWF 95 rat_12/15-LOX DSEAEFKVDVSEYLGPLLFVRVQKWH--------YLTDDAWFCNWISVKGPGDQGSEYMF 95
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mouse_12/15-LOX NSEAEFKVDVSEYLGPLLFVRVQKWH--------YLKEDAWFCNWISVKGPGDQGSEYTF 95 mouse_e12-LOX GRKTELEVDVPLHLGRLLAVKLRKQK--------GLLDSDWFCKSITVQGPGTQGEAF-F 94 mouse_p12-LOX GKEEEFDFDVPEDLGPLQFVKLHKQH--------TVVDDAWFCNLITVQGPGT-SAEAVF 94 human_p12-LOX GEEEEFDHDVAEDLGLLQFVRLRKHH--------WLVDDAWFCDRITVQGPGA-CAEVAF 94 ENSDARP00000091900 GAVDDYIVKSDTDLGPLILVRLEKHK--------YFIEDNWFCRYVKVSVPGE-RCCYTF 97 . :* : :::. *. . : * mouse_5-LOX PCYRWITGEG-EIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSID 157 rat_5-LOX PCYRWITGEG-EIVLRDGCAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSID 156 guinea_Pig_5-LOX PCYRWITGEG-EIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSID 156 human_5-LOX PCYRWITGDV-EVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSID 157 ENSDARP00000074335 PCFRWLVDDK-EVILRDGRARLPQHDKTGVAKQHRRKELEQRQKTYRWKEWHPGFPMSID 157 ENSDARP00000063256 PCYRWIADQK-ELVLPEGRGRLPQDEKLSIVKECRHLELENRQKHFRWKEWKPGFPMSID 157 ENSDARP00000063263 PCYRWIADQK-ELVLPEGRGRLPQDEKLSILKECRHLELENRREKFRWNEWKSGFLMSID 157 ENSDARP00000093033 PCYHWIADQQ-ELALPEGRGRLPQDEKLSILKQYRHKELENRQKQFRWNEWKAGLPLSID 157 human_15-LOX2 PCYQWLEGAG-TLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLD 163 mouse_8-LOX PCYQWLEGAG-ELVLREGAAKVSWQDHHPTLQDQRQKELESRQKMYSWKTYIEGWPRCLD 164 human_12R-LOX PAYQWMDGYE-TLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVH 158 mouse_12R-LOX PAYQWMDGYE-TLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVD 158 human_eLOX3 PCYQWIEGYC-TVELRPGTARTICQDSLPLLLDHRTRELRARQECYRWKIYAPGFPCMVD 290 mouse_eLOX3 PCYQWIDGYC-TVELRPGTARTICQDSLPLLLDHRKRELQARQECYRWKIFAPGFPRMVD 158 ENSDARP00000023401 PCNKWIRTADGHIILHNDKVCLKKEETLEVLTNHREKDLQCKQQIIKWGTFAEGSPHCIA 158 ENSDARP00000113298 PCYRWMTKRE-VIELREAKATKIYEEQYPQLKEHRQNELKLNKEVYRWTVFKAGLPQQAV 159 bovine_12/15-LOX PCYRWVEGD-GILSLPEGTGRTVVDDPQGLFKKHREEELAERRKLYRWGNWKDGLILNIA 154 porcine_12/15-LOX PCYRWVEGD-RILSLPEGTARTVVDDPQGLFKKHREEELAERRKLYRWGNWKDGLILNIA 154 human_12/15-LOX PCYRWVEGN-GVLSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYRWGNWKDGLILNMA 153 rabbit_12/15-LOX PCYRWVVGD-GVQSLPVGTGCTTVGDPQGLFQKHREQELEERRKLYQWGSWKEGLILNVA 154 rat_12/15-LOX PCYRWVQGR-SILSLPEGTGCTVVEDSQGLFRKHREEELEERRSLYRWGNWKDGSILNVA 154 mouse_12/15-LOX PCYRWVQGT-SILNLPEGTGCTVVEDSQGLFRNHREEELEERRSLYRWGNWKDGTILNVA 154 mouse_e12-LOX PCYSWVQGK-ETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIA 153 mouse_p12-LOX PCYRWVQGE-GILSLPEGQARLAGDNALDVFQKYREKELKERQQTYCWATWKEGLPQTIA 153 human_p12-LOX PCYRWVQGE-DILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIA 153 ENSDARP00000091900 PCYRWLVGDGVFVELREGTAKKLSEDSLPLEMAHRQSELQERQKTYRWVAWAPGIPKCID 157 *. *: * : * :: .:. * : * mouse_5-LOX A-----------------------------------------KCHKDLPRDIQFDSEKGV 176 rat_5-LOX A-----------------------------------------KCHKDLPRDIQFDSEKGV 175 guinea_Pig_5-LOX A-----------------------------------------KCHKDLPRDIQFDSEKGV 175 human_5-LOX A-----------------------------------------KCHKDLPRDIQFDSEKGV 176 ENSDARP00000074335 C-----------------------------------------KAHKELPRDIQFDSEKGV 176 ENSDARP00000063256 V-----------------------------------------NGSSELPLDVKFDTEKVV 176 ENSDARP00000063263 A-----------------------------------------NKSSELPLDVQFDTAKAV 176 ENSDARP00000093033 A-----------------------------------------NGCSELPLDVQFDTAKSV 176 human_15-LOX2 E-----------------------------------------KTVEDLELNIKYSTAKNA 182 mouse_8-LOX H-----------------------------------------ETVKDLDLNIKYSAMKNA 183 human_12R-LOX IPSYRPP----------VRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTA 208 mouse_12R-LOX IPSYHPP----------PRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTA 208 human_eLOX3 VNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNVRYSATKTI 350 mouse_eLOX3 VSSFQEMESDKKFALTKTVPCAEQDDNSGNRYLPGFPMKIDIPSLLHMEPNIRYSATKTA 218 ENSDARP00000023401 M-----------------------------------------TSVADLGPNLSYIRKRPV 177 ENSDARP00000113298 F-----------------------------------------QDPLSVPSVVRFSFTKDI 178 bovine_12/15-LOX G-----------------------------------------ATINDLPVDERFLEDKRI 173 porcine_12/15-LOX S-----------------------------------------TGIHDLPVDERFLEDKRI 173 human_12/15-LOX G-----------------------------------------AKLYDLPVDERFLEDKRV 172 rabbit_12/15-LOX G-----------------------------------------SKLTDLPVDERFLEDKKI 173 rat_12/15-LOX A-----------------------------------------ASISDLPVDQRFREDKRI 173 mouse_12/15-LOX A-----------------------------------------TSISDLPVDQRFREDKRL 173 mouse_e12-LOX G-----------------------------------------STERDLPRNQRFMKDKDL 172 mouse_p12-LOX A-----------------------------------------DCKDDLPPNMRFHEEKRL 172 human_p12-LOX A-----------------------------------------DRKDDLPPNMRFHEEKRL 172 ENSDARP00000091900 A-----------------------------------------KSEADLPQDARFDNEKRS 176 : : : mouse_5-LOX DFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVK--ISNTISERVKNHWQEDLMFG 234 rat_5-LOX DFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVK--ISNTISERVKNHWQEDLMFG 233 guinea_Pig_5-LOX DFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVK--ISNTISERVKNHWQEDLMFG 233 human_5-LOX DFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVK--ISNTISERVMNHWQEDLMFG 234 ENSDARP00000074335 DFVLNYSKAIENLYVNQFMHMFQSSWGDFADFERIFVR--IKNTISEYVMEHWKEDFMFG 234 ENSDARP00000063256 DFKLHFLKGIGNVGLNKCKGYYQS-WKDFADFEKVFIT--IENTVSEHLMQRWKEDAVFG 233 ENSDARP00000063263 DFSLNAFKAAENLGLNKLESLFQS-WKDFADFEKVFIT--IKNTESEYVMQNWTKDAVFG 233 ENSDARP00000093033 DFSVNFLEGIRILGLNKLKSVFQS-WKDFADFEKVFIT--IKNTVSEYLMENWTEDAVFG 233 human_15-LOX2 NFYLQAGSAFAEMKIKGLLDRKGL-WRSLNEMKRIFNF--RRTPAAEHAFEHWQEDAFFA 239 mouse_8-LOX KLFFKAHSAYTELKVKGLLDRTGL-WRSLREMRRLFNF--RKTPAAEYVFAHWQEDAFFA 240 human_12R-LOX SFFVRLGPMALAFKVRGLLDCKHS-WKRLKDIRKIFPG--KKSVVSEYVAEHWAEDTFFG 265 mouse_12R-LOX SFFYRLGPMALAFKLRGLVDRKRS-WKRLKDIKNIFPA--TKSVVSEYVAEHWTEDSFFG 265 human_eLOX3 SLLFNAIPASLGMKLRGLLDRKGS-WKKLDDMQNIFWC--HKTFTTKYVTEHWCEDHFFG 407 mouse_eLOX3 SLIFNALPASFGMKIRGLLDRKGS-WKRLDDIRNIFWC--HKTFTSEYVTEHWCEDSFFG 275 ENSDARP00000023401 TNVHY---------IKGFIERRTS-WRSLQELETVFLFSGSENMIAKYVQAHWQEDAFFG 227
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ENSDARP00000113298 DSAFNRSTALREIKMKKLAKKADQ-WPAIKHMKRAFWP--SKTTISKYVYLHWKDDDFLG 235 bovine_12/15-LOX DFEASLTKGLADLAIKD-SLNILTCWKSLDDFNRIFWC--GQSKLAERVRDSWKEDALFG 230 porcine_12/15-LOX DFEASLAKGLADLAVKD-SLNVLMSWNSLDSFNRIFWC--GQSKLAEQVRDSWKEDALFG 230 human_12/15-LOX DFEVSLAKGLADLAIKD-SLNVLTCWKDLDDFNRIFWC--GQSKLAERVRDSWKEDALFG 229 rabbit_12/15-LOX DFEASLAWGLAELALKN-SLNILAPWKTLDDFNRIFWC--GRSKLARRVRDSWQEDSLFG 230 rat_12/15-LOX EFEASQVIGVMDTVVNF-PINTVTCWKSLDDFNCVFKS--GHTKMAERVRNSWKEDAFFG 230 mouse_12/15-LOX EFEASQVLGTMDTVINF-PKNTVTCWKSLDDFNYVFKS--GHTKMAERVRNSWKEDAFFG 230 mouse_e12-LOX DFSLSLVKELKNFAIKG-TLDFVSRVQKLEDYQKVFPH--TKTALPERVRGSWKEDALFG 229 mouse_p12-LOX DFEWTLKAGVLEMGLKR-VYTLLRSWNHLEDFDQIFWG--QKSALAEKVHQCWQEDELFG 229 human_p12-LOX DFEWTLKAGALEMALKR-VYTLLSSWNCLEDFDQIFWG--QKSALAEKVRQCWQDDELFS 229 ENSDARP00000091900 DFEGSLHYALLELSLKKLAIRFGKSWSDLEDFRRIFWK--LRSPIAEYTMEHWKEDWFFA 234 :. : * . .. * .* .:. mouse_5-LOX YQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGID 294 rat_5-LOX YQFLNGCNPVLIKRCTELPKKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGID 293 guinea_Pig_5-LOX YQFLNGCNPVLIKRCRELPQKLPVTTEMVECSLERHLSLEQEVQEGNIFIVDYELLDGID 293 human_5-LOX YQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGID 294 ENSDARP00000074335 YQYLNGCNPVVIKKCTEIPDKFPVTHNIVEDSLERGLTLEEELKEGNIFIADYEIMDGVT 294 ENSDARP00000063256 YQFLNGCNPVMIKKCTELPGKFVVTQEMVRNSLDKGTTLQDELQAGNIYIADYEILEDVQ 293 ENSDARP00000063263 YQFLNGCNPVMIKKCTKLPDKFAVTQEMVENSLDRGTTLQEELKAGNIYIADYEILEDVQ 293 ENSDARP00000093033 YQFLNGSNPVMIKKCRKLPDKFVVTQEMVEDSLDRGTTLQEELQAGNIYIADYEILEDVQ 293 human_15-LOX2 SQFLNGLNPVLIRRCHYLPKNFPVTDAMVASLLGPGTSLQAELEKGSLFLVDHGILSGIQ 299 mouse_8-LOX SQFLNGINPVLIRRCHSLPNNFPVTDEMVAPVLGPGTSLQAELEKGSLFLVDHGILSGVH 300 human_12R-LOX YQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIP 325 mouse_12R-LOX YQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIP 325 human_eLOX3 YQYLNGVNPVMLHCISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAP 467 mouse_eLOX3 YQYLNGVNPVMLHCLSSLPSKLPVTNDMVAPLLGPGTCLQTELERGHIFLADYWILAEAP 335 ENSDARP00000023401 YQCLNGCNPLCVRQIHHLPPNFSVTSEMLKSFLPEGSSLDQEMEKGRVYLLDYEVLDQLP 287 ENSDARP00000113298 YQLLNGPNPLMLHRCSELPLNFAVSDDMLLPFLESGTSLNVEMKQGNIFLCDYKRLADLP 295 bovine_12/15-LOX YQFLNGTNPMLLRRSVRLPARLEFPPGMGE----LQAELEKELQQGTLFEADFSLLMGIK 286 porcine_12/15-LOX YQFLNGTNPMLLRHSVELPARLKFPPGMEE----LQAQLEKELQGGTLFEADFSLLDGIK 286 human_12/15-LOX YQFLNGANPVVLRRSAHLPARLVFPPGMEE----LQAQLEKELEGGTLFEADFSLLDGIK 285 rabbit_12/15-LOX YQFLNGANPMLLRRSVQLPARLVFPPGMEE----LQAQLEKELKAGTLFEADFALLDNIK 286 rat_12/15-LOX YQFLNGANPMVLKRSTCLPARLVFPPGMEK----LQAQLNKELQKGTLFEADFFLLDGIK 286 mouse_12/15-LOX YQFLNGANPMVLKRSTCLPARLVFPPGMEK----LQAQLDEELKKGTLFEADFFLLDGIK 286 mouse_e12-LOX YQFLNGANPMLLRRSMRLPARLVLPPGMED----VQTQLEKELKAGSLFEVDFSLLDGVK 285 mouse_p12-LOX YQFLNGANPMLLRRSTSLPSRLVLPSGMEE----LQAQLEKELKNGSLFEADFILLDGIP 285 human_p12-LOX YQFLNGANPMLLRRSTSLPSRLVLPSGMEE----LQAQLEKELQNGSLFEADFILLDGIP 285 ENSDARP00000091900 YQFLNGSNPRNITRLKKVPSNFLVTGDMVQSSLIPTTTLNEELKKGNIFLVDHAILDGIP 294 * *** ** : :* .: .. : *: *:: * :: *. : mouse_5-LOX ANKTDPCTHQFLAAPICLLYKNLA-NKIVPIAIQLN--QTPGESNPIFLPTDSKYDWLLA 351 rat_5-LOX ANKTDPCTHQFLAAPICLLYKNLA-NKIVPIAIQLN--QTPGEKNPIFLPTDSKYDWLLA 350 guinea_Pig_5-LOX ANKTDPCTHQFLAAPICLLYKNLA-NKIVPIAIQLN--QAPGEKNPIFLPSDAKYDWLLA 350 human_5-LOX ANKTDPCTLQFLAAPICLLYKNLA-NKIVPIAIQLN--QIPGDENPIFLPSDAKYDWLLA 351 ENSDARP00000074335 ANSTDPCTLQYLAAPMCLLYKNSQ-NKIMPIAIQLH--QKPGEDNPIFLPSDDEYDWLLA 351 ENSDARP00000063256 PNDTDPSTQQYLAAPFCLFYKNGQ-NKFIPIAIQLSREATPGQMNTVFLPSDNHYDWLLA 352 ENSDARP00000063263 PNDTDPSTQQYLATPFCLFYKNGQ-NEFIPIAIQLSRQTTPGEMNTVFLPTDDEYDWLLA 352 ENSDARP00000093033 PNDTDSSTRQYLAAPFCLFYKNSQ-NKIIPIAIQLSREATPGQKNTVFLPSDNHYDWLLA 352 human_15-LOX2 TNVIN-GKPQFSAAPMTLLYQSPGCGPLLPLAIQLS--QTPGPNSPIFLPTDDKWDWLLA 356 mouse_8-LOX TNILN-GKPQFSAAPMTLLHQSSGSGPLLPIAIQLK--QTPGPDNPIFLPSDDTWDWLLA 357 human_12R-LOX TVELS-GRKQHHCAPLCLLHFGPE-GKMMPIAIQLS--QTPGPDCPIFLPSDSEWDWLLA 381 mouse_12R-LOX TVELN-GQQQHHCAPMCLLHFGPD-GNMMPIAIQLS--QTPGPDCPIFLPNDSEWDWLLA 381 human_eLOX3 THCLN-GRQQYVAAPLCLLWLSPQ-GALVPLAIQLS--QTPGPDSPIFLPTDSEWDWLLA 523 mouse_eLOX3 VHCIN-GLQQYVTAPLCLLWLNPQ-GVLLPLAIQLS--QTPGPESPIFLPTDCELDWLLA 391 ENSDARP00000023401 ANTVN-GKQTYLSAPLCLLHYNQD-EELKPIAIQLQ--QVPGPQNPIFLPSDAPPDWLLA 343 ENSDARP00000113298 TQVIN-GKQQFVTAPLCLLYKNQE-GKLLPIAIQLQ--QEASEHSPVFLPSDSELDWLTA 351 bovine_12/15-LOX ANVIL-CTQQYVAAPLVMLKLQPD-GKLLPMAIQLQLPHKGSPPPPLFLPTDPPMTWLLA 344 porcine_12/15-LOX ANVIL-CSQQYLAVPLVMLKLQPD-GKLLPMVIQLQLPREGSPLPPLFLPTDPPMVWLLA 344 human_12/15-LOX ANVIL-CSQQHLAAPLVMLKLQPD-GKLLPMVIQLQLPRTGSPPPPLFLPTDPPMAWLLA 343 rabbit_12/15-LOX ANVIL-YCQQYLAAPLVMLKLQPD-GKLMPMVIQLHLPKIGSSPPPLFLPTDPPMVWLLA 344 rat_12/15-LOX ANVIL-CSQQYLAAPLVMLKLMPD-GQLLPIAIQLELPKTGSTPPPIFTPSDPPMDWLLA 344 mouse_12/15-LOX ANVIL-CSQQYLAAPLVMLKLQPD-GQLLPIAIQLELPKTGSTPPPIFTPLDPPMDWLLA 344 mouse_e12-LOX PNIII-FKQQYVTAPLVMLKLQPD-GRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLA 343 mouse_p12-LOX ANVIR-GEPQYLAAPLVMLRMDPG-GKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLA 343 human_p12-LOX ANVIR-GEKQYLAAPLVMLKMEPN-GKLQPMVIQIQPPSPSSPTPTLFLPSDPPLAWLLA 343 ENSDARP00000091900 ANVIR-NSPQYIAAPLCLLYEHPE-KGLIPIAIQLE--QKPDKDTPVFLPSDPPLAWLLA 350 . .*: :: : *:.**: :* * * ** * mouse_5-LOX KIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKA 411 rat_5-LOX KIWVRSSDFHIHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKA 410 guinea_Pig_5-LOX KIWVRSSDFHVHQTITHLLCTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKA 410 human_5-LOX KIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKA 411 ENSDARP00000074335 KIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLPAVHPVFKLLLPHIRFTIAINTKA 411 ENSDARP00000063256 KMWVKSCDFNVHQLVTHLLRTHLISEVFAIAMFRQLSAVHPVYKLLIPHVRFTIAINTAA 412 ENSDARP00000063263 KMWVKSSDYNVHQLVTHLLRTHLISEVFTIAMFRQLSAVHPVYKLLIPHVRFTIAINTAA 412 ENSDARP00000093033 KMWVKSCDFNVHQLITHLLRTHLTSEVFAIAMFRQLSAVHPVYKLLIPHVRFTIAINTSA 412 human_15-LOX2 KTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLA 416
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mouse_8-LOX KTWVRNSEFYIHEAVTHLLHAHLIPEVFALATLRQLPRCHPLFKLLIPHIRYTLHINTLA 417 human_12R-LOX KTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIG 441 mouse_12R-LOX KTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIG 441 human_eLOX3 KTWVRNSEFLVHENNTHFLCTHLLCEAFAMATLRQLPLCHPIYKLLLPHTRYTLQVNTIA 583 mouse_eLOX3 KTWVRNSEFLVHENNTHFLCTHLLCEAFSMATLRQLPLCHPVYKLLLPHTRYTLQVNTIA 451 ENSDARP00000023401 KMWVRNSDFQIHQLLSHFFRTHLLGEVCCTATLRQLPEIHPLFQLLMPHIRSTLQINIQA 403 ENSDARP00000113298 KLYVRNADCLEHQAALHLR--SLLAETFTLSLLRNLPTVHPIHKLLVPHIRYTLQMNSLV 409 bovine_12/15-LOX KCWVRSSDFQLHELHSHLLRGHLVAEVIAVATMRCLPSIHPMFKLLIPHLRYTMEINIRA 404 porcine_12/15-LOX KCWVRSSDFQLHELHSHLLRGHLMAEVIAVATMRCLPSIHPIFKLLIPHFRYTMEINVRA 404 human_12/15-LOX KCWVRSSDFQLHELQSHLLRGHLMAEVIVVATMRCLPSIHPIFKLIIPHLRYTLEINVRA 403 rabbit_12/15-LOX KCWVRSSDFQVHELNSHLLRGHLMAEVFTVATMRCLPSIHPVFKLIVPHLRYTLEINVRA 404 rat_12/15-LOX KCWVRSSDLQLHELQAHLLRGHLMAELFAVATMRCLPSVHPVFKLLVPHLLYTMEINVRA 404 mouse_12/15-LOX KCWVRSSDLQLHELQAHLLRGHLVAEVFAVATMRCLPSVHPVFKLLVPHLLYTMEINVRA 404 mouse_e12-LOX KIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLA 403 mouse_p12-LOX KIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRT 403 human_p12-LOX KSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRA 403 ENSDARP00000091900 KMWVRHAEFQVFQLLSHLLRTHLMVEVICVATLRQLPAVHPIYKLLTPHLRYTLEINCRG 410 * :*: .: : *: * * : * *. **:.::: .* .: :* mouse_5-LOX REQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDSTEDMPFYFY 471 rat_5-LOX REQLNCEYGLFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDNTEDIPYYFY 470 guinea_Pig_5-LOX REQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDSTEDIPYYFY 470 human_5-LOX REQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFY 471 ENSDARP00000074335 REQLICECGLFDKANGTGGGGHIELVQRSMKVFTYKSLCFPENIKARGMDSQEELPYYFY 471 ENSDARP00000063256 REKLINDTGVFNEANSSGGIGLQQVIQKAMQTLTYKSLCFPEAMKTRGVDNQEDLPNYYY 472 ENSDARP00000063263 RETLINEGGVFGKVDSTGGIGIEQVIRKGMQTLTYKSLCFPEAMKIRNVDNKEDLPNYYY 472 ENSDARP00000093033 RQTLINDTGVFNKANSTGGIGVLQVVHRAMKTLTYKSMCFPEAMKARDVHDQEDLPNYYY 472 human_15-LOX2 RELLIVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVED---IPGYYY 473 mouse_8-LOX RELLVAPGKLIDKSTGLGTGGFSDLIKRNMEQLNYSVLCLPEDIRARGVED---IPGYYY 474 human_12R-LOX RAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQD---LPGYYY 498 mouse_12R-LOX RALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQD---LPGYYF 498 human_eLOX3 RATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFCLPDSLRARGVLA---IPNYHY 640 mouse_eLOX3 RATLLNPDGLVDKVTSIGRQGLIYLMSTGLAHFTYTDFCLPDSIRARGVLT---IPNYHY 508 ENSDARP00000023401 RATLLAANGVFDKSVACGLEAIPQLFTRATQRLRYSSLCVPDDVKERGLDA---LPVCYY 460 ENSDARP00000113298 RHRLIGPDGVLSQCTSIGHEGMMALMRKALSDLTYSSLCLPEDISGRQLDS---LPNFFY 466 bovine_12/15-LOX RTGLVSDSGVFDQVVSTGGGGHVELLQRAGAFLTYSSFCPPDDLADRGLLG---VKSSFY 461 porcine_12/15-LOX RNGLVSDLGIFDQVVSTGGGGHVELLRRAAALLTYSSFCPPDDLADRGLLG---VESSFY 461 human_12/15-LOX RTGLVSDMGIFDQIMSTGGGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLG---VKSSFY 460 rabbit_12/15-LOX RNGLVSDFGIFDQIMSTGGGGHVQLLQQAGAFLTYRSFCPPDDLADRGLLG---VESSFY 461 rat_12/15-LOX RSDLISERGFFDKAMSTGGGGHLDLLKQAGAFLTYCSLCPPDDLAERGLLD---IETCFY 461 mouse_12/15-LOX RSDLISERGFFDKVMSTGGGGHLDLLKQAGAFLTYSSLCPPDDLAERGLLD---IDTCFY 461 mouse_e12-LOX RNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVG---VKSSLY 460 mouse_p12-LOX RTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLR---IPSALY 460 human_p12-LOX RTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLG---LPGALY 460 ENSDARP00000091900 RTQLLSPEGIFKRVVSTGGEGLLILAQREYKVLTYRSLQPKFDFLDRGVTK---VKGYVY 467 * * . . * . : : * : . * : : : mouse_5-LOX RDDGLLVWEAIQSFTMEVVSIYYENDQVVEEDQELQDFVKDVYVYGMRGKKASG-FPKSI 530 rat_5-LOX RDDGLLVWEAIQSFTTEVVSIYYEDDQVVEEDQELQDFVKDVYVYGMRGRKASG-FPKSI 529 guinea_Pig_5-LOX RDDGLLVWEAIQSFTSEVVSIYYEDDQVVMEDQELQDFVKDVYVYGMRGRKASG-FPKSI 529 human_5-LOX RDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSG-FPKSV 530 ENSDARP00000074335 RDDGCAVWETVKSFVSDVVDIYYDKDETVQEDEEIQAFVKDVCSFGMQDLDHCE-FPKSL 530 ENSDARP00000063256 RDDGMLVWEAVKSFVSDVVKIYYSSDETVQGDEEIQAFVQDVCSSGMKNCPKNGEFPSAL 532 ENSDARP00000063263 RDDGMMVWEAVKSFVSDVVKFYYSSDETVQEDEEIQAFVQDVCSYGMKNCPNNGEFPNAL 532 ENSDARP00000093033 RDDGMLVWEAVKSFVSDVVEIYYSSDETVLEDEEIQAFVQDVHSSGMKNCPNSGEFPNML 532 human_15-LOX2 RDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSG-IPSSL 532 mouse_8-LOX RDDGMQIWGAIKSFVSEIVSIYYPSDTSVQDDQELQAWVREIFSEGFLGRESSG-MPSLL 533 human_12R-LOX RDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSG-FPRCL 557 mouse_12R-LOX RDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSG-FPTCL 557 human_eLOX3 RDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRESSG-FPSRL 699 mouse_eLOX3 RDDGLKIWAAIERFVSEIVSYYYPSDASVQQDCELQAWVGEIFAQAFLGRESSG-FPSRL 567 ENSDARP00000023401 AQDALRVWDALHRFVAGWTRLYYCSDEDVQSDCELQNWIGEIFREGFLGLTHLG-VPQSF 519 ENSDARP00000113298 RDDGLRVWAFINDFVRSVVEFFYQNDSDVQKDTELQDWINDIFIHGFLEQSSSG-IPQSF 525 bovine_12/15-LOX AQDALRLWEILSRYVEGIVSLHYKTDESVRDDIELQAWCRDITEIGLLGAQDRG-FPVTL 520 porcine_12/15-LOX AQDALRLWEVISRYVEGIVSLHYKTDESVKEDFELQAWCREFTEIGLLGAQDRG-FPVSL 520 human_12/15-LOX AQDALRLWEIIYRYVEGIVSLHYKTDVAVKDDPELQTWCREITEIGLQGAQDRG-FPVSL 519 rabbit_12/15-LOX AQDALRLWEIISRYVQGIMGLYYKTDEAVRDDLELQSWCREITEIGLQGAQKQG-FPTSL 520 rat_12/15-LOX AKDALRLWQIMNRYVVGMFNLHYKTDKAVQDDYELQSWCREITDIGLQGAQDRG-FPTSL 520 mouse_12/15-LOX AKDALQLWQVMNRYVVGMFDLYYKTDQAVQDDYELQSWCQEITEIGLQGAQDRG-FPTSL 520 mouse_e12-LOX AQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRG-FPLSL 519 mouse_p12-LOX ARDALQLWEVTARYVKGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRG-FPVSF 519 human_p12-LOX AHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRG-FPVSF 519 ENSDARP00000091900 RDYSLMLWDVIQNFVSGIVSLYYQCDSDVQEDSELQAWIHDVAVEGFVDVPEFG-LASDM 526 . :* :. .* * * * *:* : :. : .. . mouse_5-LOX KSREKLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVD 590 rat_5-LOX KSREKLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVD 589 guinea_Pig_5-LOX KSREKLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPATAKGVVTIEQIVA 589
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human_5-LOX KSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVD 590 ENSDARP00000074335 QTKEELVEYLTVVIFTASAQHAAVNFGQYDWCSWIPNSPPTMRKPPPMKKGEVDLQYIVE 590 ENSDARP00000063256 KTQEQLVEYLTVVIFTASAQHAAVNFGQFDWFAWVPNTPSTMRKPPPTEKGKVDMKYIME 592 ENSDARP00000063263 KTREQLVEYLTIVIFTASAQHAAINFGQFDWYAWIPNTPSTMRKPPPIEKGKVDMKYIME 592 ENSDARP00000093033 RTREQLVEYLTVMIFTASAQHAAVNFGQFDWFAWVPNTPSTMRKPPPTEKGKVDMKYIME 592 human_15-LOX2 ETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIA 592 mouse_8-LOX DTREALVQYITMVIFTCSAKHAAVSSGQFDSCVWMPNLPPTMQLPPPTSKGQARPESFIA 593 human_12R-LOX RTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMD 617 mouse_12R-LOX RTIPELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMD 617 human_eLOX3 CTPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLD 759 mouse_eLOX3 CTPGELVKYLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGDTTMKSYLD 627 ENSDARP00000023401 QTAAEMCKFVTMVIFSCSALHSAVNFSQLDFNLCIPNTPATMSRPPSQTKGSVSEEDLLS 579 ENSDARP00000113298 STVEELIKFLTMMIFTVSAQHAALRAGQFDYGSWFPNTPGSLKSPPPACKGSSDKNTVLQ 585 bovine_12/15-LOX QSKDQLCHFVTMCIFTCTGQHSSTHLGQLDWYSWVPNAPCTMRLPPPTTK-DVTLEKVMA 579 porcine_12/15-LOX QSKEQLCHFVTMCIFTCTGQHSSNHLGQLDWYTWVPNAPCTMRLPPPTTK-DATLETVMA 579 human_12/15-LOX QARDQVCHFVTMCIFTCTGQHASVHLGQLDWYSWVPNAPCTMRLPPPTTK-DATLETVMA 578 rabbit_12/15-LOX QSVAQACHFVTMCIFTCTGQHSSIHLGQLDWFTWVPNAPCTMRLPPPTTK-DATLETVMA 579 rat_12/15-LOX QSRAQACYFITMCIFTCTAQHSSVHLGQLDWFYWVPNAPCTMRLPPPTTK-EATMEKLMA 579 mouse_12/15-LOX QSRAQACHFITMCIFTCTAQHSSIHLGQLDWFYWVPNAPCTMRLPPPKTK-DATMEKLMA 579 mouse_e12-LOX ESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISK-DVTERDIVD 578 mouse_p12-LOX QSRAQLCHFLTMCVFTCTAQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETVMG 579 human_p12-LOX QSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMG 579 ENSDARP00000091900 KTKEELITLLSVAIFTSTAQHAATNNGQFDWCAWVPNTPCTMRHPPPRDKDAVTMEMIMD 586 : :: ::. :. *:: .* : .** * :: ** * : mouse_5-LOX TLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIISVIA 650 rat_5-LOX TLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIVSVIA 649 guinea_Pig_5-LOX TLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMTRFRKNLEAIVNVIA 649 human_5-LOX TLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIA 650 ENSDARP00000074335 SLPDRGRSCWHLGAVWALSQFQENELFLGMYPDEHFIEKPVKEVMVKFGKKLAEVSKTIK 650 ENSDARP00000063256 SLPDRQRSSWHIGAVWALSQFQDSELFLGEYPDKYFTEKLVTEAIENFRKELFVITKIIR 652 ENSDARP00000063263 SLPDHGRSSLHLGTIWILSQFQDNELFLGVYPDKYFTEEPAMEAIKNFRKKLVDVTKTIR 652 ENSDARP00000093033 SLPDRGRSGWHLGALWSLSQFQDNELFLGEYPDKYFTEEPAIEAIKNFRKTLVDITKIIK 652 human_15-LOX2 TLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQ 652 mouse_8-LOX TLPAVNSSSYHIIALWLLSAEPGDQRPLGHYPDEHFTEDAPRRSVAAFQRKLIQISKGIR 653 human_12R-LOX TLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIR 677 mouse_12R-LOX TLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIR 677 human_eLOX3 TLPEVNISCNNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQ 819 mouse_eLOX3 TLPEVNTTCRNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRQSIAAFQNCLAQISKDIR 687 ENSDARP00000023401 FLPEVNSSCHVLSVLSLLSQPAIDFVPLCHYNEWYFHSGAMAKLVEDVQKELKIIAKDIA 639 ENSDARP00000113298 ALPDINTSANLASVFWLLSKPSADLVPLGQYPEDYFCQTAVRKMMRDFQAELAFFSEYVE 645 bovine_12/15-LOX TLPNFHQASLQMSITWQLGRRQPIMVALGQHEEEYFSGPEPKAVLKKFREELAALEKDIE 639 porcine_12/15-LOX TLPNFHQASLQMSITWQLGRCQPTMVALGQHEEEYFSGPGPKAVLTKFREELAALDKDIE 639 human_12/15-LOX TLPNFHQASLQMSITWQLGRRQPVMVAVGQHEEEYFSGPEPKAVLKKFREELAALDKEIE 638 rabbit_12/15-LOX TLPNLHQSSLQMSIVWQLGRDQPIMVPLGQHQEEYFSGPEPRAVLEKFREELAIMDKEIE 639 rat_12/15-LOX TLPNPNQSTLQINVVWLLGRRQAVMVPLGQHSEEHFPNPEAKAVLKKFREELAALDKEIE 639 mouse_12/15-LOX TLPNPNQSTLQINVVWLLGRRQAVMVPLGQHSEEHFPNPEAKAVLKKFREELAALDKEIE 639 mouse_e12-LOX SLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIE 638 mouse_p12-LOX SLPDVQKACLQMTITWHLGRLQPDMVPLGHHTEKYFSDPRTKAVLSQFQADLDNLEKEIT 639 human_p12-LOX SLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEIT 639 ENSDARP00000091900 TLPDISQSCVQMAITWHLGRAQPDAIPMGQYVEQYFTEPAALKVIDKFRKELKELEDKIM 646 ** : : : . : :* : . * . : mouse_5-LOX ERNKNKKLPYYYLSPDRIPNSVAI 674 rat_5-LOX ERNKNKKLPYYYLSPDRIPNSVAI 673 guinea_Pig_5-LOX ERNKNKKLPYYYLSPDRIPNSVAI 673 human_5-LOX ERNKKKQLPYYYLSPDRIPNSVAI 674 ENSDARP00000074335 NRNEGKKLPYYYLSPDRIPNSVAV 674 ENSDARP00000063256 KRNETLELPYLYLCPDRIPNSVAI 676 ENSDARP00000063263 RRNETLELPYWYLCPDRIPNSVAI 676 ENSDARP00000093033 KRNETLELPYWYLSPDRIPNSVAI 676 human_15-LOX2 ERNRGLVLPYTYLDPPLIENSVSI 676 mouse_8-LOX ERNRGLALPYTYLDPPLIENSVSI 677 human_12R-LOX QRNKCLPIPYYYLDPVLIENSISI 701 mouse_12R-LOX QRNKCLTLPYYYLDPVLIENSISI 701 human_eLOX3 ERNQGLALPYTYLDPPLIENSVSI 843 mouse_eLOX3 ERNQSLALPYAYLDPPLIENSVSI 711 ENSDARP00000023401 DRNSQLELPYPYMSPDYIENSVTI 663 ENSDARP00000113298 DRNADLLVPYKYMTPENICNSVSV 669 bovine_12/15-LOX IRNAQLDWPYEYLRPSLVENSVAI 663 porcine_12/15-LOX VRNAKLALPYEYLRPSRVENSVAI 663 human_12/15-LOX IRNAKLDMPYEYLRPSVVENSVAI 662 rabbit_12/15-LOX VRNEKLDIPYEYLRPSIVENSVAI 663 rat_12/15-LOX IRNKSLDIPYEYLRPSMVENSVAI 663 mouse_12/15-LOX IRNKSLDIPYEYLRPSLVENSVAI 663 mouse_e12-LOX VRNASLDLPYEYLRPSLVENSVTI 662 mouse_p12-LOX ARNEQLDLPYEYLKPSRIENSITI 663 human_p12-LOX ARNEQLDWPYEYLKPSCIENSVTI 663
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ENSDARP00000091900 AQNEGLELQYLYLCPSRMENSITI 670 :* * *: * : **:::
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Supplemental Fig. S2. Phylogenetic tree suggesting the evolutionary relatedness of the different zebrafish (D. rerio) LOX isoforms. The tree was constructed on the basis of the publically available sequence data.
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Supplemental Fig. S3. Overlay of the crystal structures of rabbit 12/15-LOX and human 5-LOX indicating the position of the critical Ala in the two enzymes. The two structures of human 5-LOX (3O8Y, molecule A) and rabbit 15-LOX (2P0M, molecule B) were aligned (VMD software package) and the surface of human 5-LOX was calculated using a 1.4 Å probe. After zoomig into the molecule, internal cavities appear solid. The cavity between Ala411 (blue) and iron (orange) might be part of the fatty acid binding site. The same space is partially occupied by the inhibitor RS7 in the 15-LOX structure (not shown). For comparison the very similar positions of iron (red) and Ala404 (green) of the rabbit 12/15-LOX are shown. VMD-citations: W. Humphrey, A. Dalke, K. Schulten, J. Mol. Graphics 14 (1996) 33–38. R.B. Russell, G.J. Barton, Proteins 14 (1992) 309–323. A. Varshney, F.P. Brooks, W.V. Wright, IEEE Comput. Graphics Appl. 14 (1994)19–25.
