Front MatterSource: Proceedings of the National Academy of Sciences of the United States of America,Vol. 78, No. 2, [Part 2: Biological Sciences] (Feb., 1981), pp. i-xiiiPublished by: National Academy of SciencesStable URL: http://www.jstor.org/stable/10271 .

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Proceedings OF THE

National Academy of Sciences

OF THE UNITED STATES OF AMERICA

February 1981 Volume 78, Number 2 pp. 659-1308

Table of Contents

INFORMATION TO CONTRIBUTORS ix-xii

AUTHOR INDEX xiii-xiv

Physical Sciences 659-706

Biological Sciences

BIOCHEMISTRY

Movement and site selection for priming by the primosome in phage 4X174 DNA Ken-ichi Arai, Robert L. Low, and 707-711 replication (protein n'/dnaB protein/DNA -dependent ATPase/replication Arthur Kornberg promoter/processivity)

Fine structural analysis of the chicken pro ca2 collagen gene [a2 (type I) collagen John Wozney, Douglas Hanahan, 712-716 gene/Southern blot restriction endonuclease mapping/DNA sequence Richard Morimoto, Helga determination of exons and introns] Boedtker, and Paul Doty

Lysosomotropic amines inhibit mitogenesis induced by growth factors (epidermal A. Christie King, Lydia Hernaez- 717-721 growth factor/insulin/receptor-mediated degradation/internalization) Davis, and Pedro Cuatrecasas

Structure of the turkey erythrocyte adenylate cyclase system (receptors/ Thor B. Nielsen, Pramod M. Lad, M. 722-726 membranes/hormone action) Sue Preston, Ellis Kempner,

Werner Schlegel, and Martin Rodbell

Specific transcription of mouse ribosomal DNA in a cell-free system that mimics Ingrid Grummt 727-731 control in vivo (recombinant DNA/ribosomal genes/cell-free extracts/RNA polymerase I/transcription control)

Hormone-sensitive lipase in differentiated 3T3-L1 cells and its activation by cyclic Masahiro Kawamura, Dennis F. 732-736 AMP-dependent protein kinase (lipolysis) Jensen, Edward V. Wancewicz,

Lorna L. Joy, John C. Khoo, and Daniel Steinberg

Developmental regulation of secretory protein synthesis in rat seminal vesicle Malathi K. Kistler, Michael C. 737-741 (androgen/cell-free translation/mR NA) Ostrowski, and W. S. Kistler

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Contents

Replication of herpes simplex virus DNA: Localization of replication recognition Donald A. Vlazny and Niza Frenkel 742-746 signals within defective virus genomes (rolling circle/replicative origin/DNA processing/transfection)

Amino acid and carbohydrate structural variants of glycoprotein products (M-N Olga 0. Blumenfeld, Anthony M. 747-751 glycoproteins) of the M-N allelic locus (erythrocytes/glycophorins/ Adamany, and Karen V. Puglia oligosaccharides/peptide polymorphism/carbohydrate variants)

Mechanism of araC autoregulation and the domains of two overlapping Nancy L. Lee, William 0. Gielow, and 752-756 promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia Ron G. Wallace coli (DNA-protein contacts/cyclic AMP-binding protein/araC proteinl transcription)

Synthesis of [a-methyltyrosine-4]angiotensin II: Studies of its conformation, M. C. Khosla, K. Stachowiak, R. R. 757-760 pressor activity, and mode of enzymatic degradation (angiotensin II analog/ Smeby, F. M. Bumpus, F. Piriou, resistance to a-chymotrypsin degradation/full pressor activity/NMR and K. Lintner, and S. Fermandjian circular dichroism spectra similar to those of angiotensin II)

Activation of the heat-stable polypeptide of the ATP-dependent proteolytic system Aaron Ciechanover, Hannah Heller, 761-765 (ubiquitin/adenylate/thiolester/high-energy bond) Rachel Katz-Etzion, and Avram

Hershko

Immunological identification of a common precursor to arginine vasopressin and Hartwig Schmale and Dietmar 766-769 neurophysin II synthesized by in vitro translation of bovine hypothalamic Richter mRNA (signal sequence/polyprotein/processing/core glycosylation/ tunicamycin)

Comparison of [3H]glucosamine-labeled glycoproteins from human renal cancer Shun-Ichiro Ogata, Ryuzo Ueda, and 770-774 and normal kidney epithelial cell cultures by two-dimensional polyacrylamide Kenneth 0. Lloyd gel electrophoresis (carbohydrate differences/fetal kidney)

Quantitation of cation transport by reconstituted membrane vesicles containing Wilson C.-S. Wu, Hsiao-Ping H. 775-779 purified acetylcholine receptor (reconstitution/cation flux/single-channel Moore, and Michael A. Raftery transport/stopped-flow spectroscopy)

Kinetics and mechanism of heme-induced refolding of human a-globin (folding Yvonne Leutzinger and Sherman 780-784 pathways/domains/stopped-flow circular dichroism) Beychok

Complete amino acid sequence of the Fc region of a human 8 chain Tomotaka Shinoda, Nobuhiro 785-789 (immunoglobulin D/internal homology/domain origin/evolution) Takahashi, Tatsunori Takayasu,

Tsuneo Okuyama, and Akira Shimizu

Complete amino acid sequence of a 50,000-dalton fragment of human Francis E. Dwulet and Frank W. 790-794 ceruloplasmin (protein structure/plasma proteins/copper oxidase/internal Putnam homology/gene evolution)

Local anesthetics, mepacrine, and propranolol are antagonists of calmodulin M. Volpi, R. I. Sha'afi, P. M. Epstein, 795-799 (erythrocyte membrane calcium ATPase/cyclic nucleotide) D. M. Andrenyak, and M. B.

Feinstein Isolation and characterization of a cloned DNA sequence associated with the Masahiko Negishi, David C. Swan, 800-804

murine Ah locus and a 3-methylcholanthrene-induced form of cytochrome Lynn W. Enquist, and Daniel W. P-450 (recombinant DNA/pBR322 plasmid DNA/cytochrome P, -450 gene/ Nebert albumin gene)

Two small RNAs encoded by Epstein-Barr virus and complexed with protein are Michael R. Lerner, Nancy C. 805-809 precipitated by antibodies from patients with systemic lupus erythematosus Andrews, George Miller, and Joan (Epstein-Barr virus-encoded RNA/La antigen/small nuclear A. Steitz ribonucleoprotein)

Abundant pseudogenes for small nuclear RNAs are dispersed in the human Richard A. Denison, Scott W. Van 810-814 genome (multigene families/Alu family sequence) Arsdell, Laurel B. Bernstein, and

Alan M. Weiner

Expression of herpes simplex virus type I thymidine kinase gene in Escherichia Axel C. Garapin, Florence Colbere- 815-819 coli (selective marker/gene cloning/translation/DNA sequence/lac operon) Garapin, Michel Cohen-Solal,

Florian Horodniceanu, and Philippe Kourilsky

DNA sequence required for initiation of transcription in vitro from the major late Shiu-Lok Hu and James L. Manley 820-824 promoter of adenovirus 2 (in vitro mutagenesis/RNA polymerase II/RNA cap site/eukaryotic promoter)

Reaction site of carboxanilides and of thenoyltrifluoroacetone in complex II Rona R. Ramsay, Brian A. C. Ackrell, 825-828 (carboxin/photoaffinity labeling/succinate dehydrogenase) Christopher J. Coles, Thomas P.

Singer, Gordon A. White, and G. Denis Thorn

Multiple functional domains of the heparin molecule (mucopolysaccharide/ Gary M. Oosta, William T. Gardner, 829-833 anticoagulant function) David L. Beeler, and Robert D.

Rosenberg

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Contents

Covalent modification and repressed transcription of a gene in hepatoma cells Hira L. Nakhasi, Kevin R. Lynch, 834-837 (tumor gene structure/DNA methylationiDNA modification/restriction Kevin P. Dolan, Ronald D. endonuclease/Southern blots) Unterman, and Philip Feigelson

HeLa cell DNA polymerase a is tightly associated with tryptophanyl-tRNA Eliezer Rapaport, Paul C. Zamecnik, 838-842 synthetase and diadenosine 5',5"_'-Pl,P4-tetraphosphate binding activities and Earl F. Baril (DNA replication/protein synthesis/growth regulation/L-tryptophan/DNA nucleotidyltransferase)

Catenation and knotting of duplex DNA by type 1 topoisomerases: A mechanistic Patrick 0. Brown and Nicholas R. 843-847 parallel with type 2 topoisomerases (nicking-closing enzyme/w protein/linking Cozzarelli number/DNA condensation/circular DNA)

Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle Shozo Shoji, David C. Parmelee, 848-851 cyclic AMP-dependent protein kinase (regulatory enzyme/phosphoserine/ Roger D. Wade, Santosh Kumar, phosphothreonine/metabolic control) Lowell H. Ericsson, Kenneth A.

Walsh, Hans Neurath, George L. Long, Jacques G. Demaille, Edmond H. Fischer, and Koiti Titani

Release of 7-methylguanine residues from alkylated DNA by extracts of Jacques Laval, Josiane Pierre, and 852-855 Micrococcus luteus and Escherichia coli (DNA repair/DNA glycosylase/3- Frangoise Laval methyladenine/base excision)

Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 B. Singer and T. P. Brent 856-860 methyl and ethyl purines but not 06-alkylguanines of 1-alkyladenines (7- methylguanine DNA glycosylase/ethyl purine repair/analysis for alkyl bases by high-performance liquid chromatography)

Enzymatic release of 7-methylguanine from methylated DNA by rodent liver Geoffrey P. Margison and Anthony E. 861-865 extracts (3-methyladenine/DNA repair/glycosylases/alkylating agents) Pegg

Relationship between phosphorylation and activity of heme-regulated eukaryotic Remi Fagard and Irving M. London 866-870 initiation factor 2a kinase (inhibition of protein synthesis/binding of hemin)

Mechanism of activation of cyclic nucleotide phosphodiesterase: Requirement of Charles Y. Huang, Vincent Chau, P. 871-874 the binding of four Ca24 to-calmodulin for activation (kinetic analysis/Ca2" Boon Chock, Jerry H. Wang, and regulation/cooperative phenomenon/cellular control) Rajendra K. Sharma

Identification of the glucagon receptor in rat liver membranes by photoaffinity Gary L. Johnson, Vincent I. 875-878 crosslinking (hydroxysuccinimidyl-p-azidobenzoate/covalent attachment/ MacAndrew, Jr., and Paul F. Pilch hormones)

Specific 5' flanking sequences are required for faithful initiation of in vitro Sophia Y. Tsai, Ming-Jer Tsai, and 879-883 transcription of the ovalbumin gene (deletion mutants/Hogness box/eukaryotic Bert W. O'Malley promoter)

Replication of adenovirus DNA-protein complex with purified proteins (adenoviral Joh-E Ikeda, Takemi Enomoto, and 884-888 DNA replication/adenovirus DNA binding protein/5'-terminal protein/ Jerard Hurwitz eukaryotic DNA polymerase)

DNA sequence of the transfer RNA region of bacteriophage T4: Implications for Gail P. Mazzara, Guy Plunkett III, 889-892 transfer RNA synthesis and William H. McClain

Effect of phenobarbital on the level of translatable rat liver epoxide hydrolase Cecil B. Pickett and Anthony Y. H. 893-897 mRNA (drug-metabolizing enzymes/phenobarbital induction/cell-free protein Lu synthesis/immunoprecipitation)

Metal cation influence on activity and regulation of asparatate Richard B. Honzatko, Ann M. 898-902 carbamoyltransferase (enzyme kinetics/allosteric enzyme/metal-nucleotide Lauritzen, and William N. interactions/enzyme regulation) Lipscomb

Initiation sites for discontinuous DNA synthesis of bacteriophage T7 (RNA Asao Fujiyama, Yuji Kohara, and 903-907 priming/DNA sequence/mapping of RNA-DNA junctions/RNA -linked DNA Tuneko Okazaki pieces/signal for initiation)

Translational stability of native and deadenylylated rabbit globin mRNA injected George Huez, Claudine Bruck, and 908-911 into HeLa cells [ca and 13 globin messenger RNA/poly(A)/cell injection/two- Yvette Cleuter dimensional gel electrophoresis]

Early events in herpes simplex virus type 1 infection: Photosensitivity of Neal DeLuca, David Bzik, Stanley 912-916 fluorescein isothiocyanate-treated virions (glycoprotein crosslinking/virus Person, and Wallace Snipes entry/virus photosensitization)

Antifolate-induced misincorporation of deoxyuridine monophosphate into DNA: W. David Sedwick, Marc Kutler, and 917-921 Inhibition of high molecular weight DNA synthesis in human lymphoblastoid Oliver E. Brown cell s (metoprine/DNA synthesis/lipid -soluble antifolate)

Molecular basis of valine resistance in Escherichia coli K-12 (DNA sequence/ Robert P. Lawther, David H. 922-925 frameshift mutation/transcriptional polarity/isoleucine and valine biosynthesis) Calhoun, Craig W. Adams, Craig

A. Hauser, John Gray, and G. Wesley Hatfield

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Contents

Specific recognition of apurinic sites in DNA by a tryptophan-containing peptide Tula Behmoaras, Jean-Jacques 926-930 (peptide-DNA complexes/stacking interaction/tryptophan fluorescencel Toulme, and Claude Helene alkylating agents/DNA repair)

Myosin light chains and the developmental origin of fast muscle (skeletal muscle/ Frank E. Stockdale, Neerja Raman, 931-935 pectoral muscle/slow.and fast fiber type/differentiation) and Helen Baden

Stimulation of hepatic glutathione formation by administration of L-2- Joanne M. Williamson and Alton 936-939 oxothiazolidine-4-carboxylate, a 5-oxo-L-prolinase substrate (cysteine delivery Meister system/phosgene/pyroglutamate)

Specificity of chemiluminescence in the metabolism of benzo[a]pyrene to its J. P. Hamman, H. H. Seliger, and G. 940-942 carcinogenic diol epoxide (microsomal chemiluminescence/chemiluminescence H. Posner quantum yields/dioxetane of benzo[a]pyrene)

Simian virus 40 tandem repeated sequences as an element of the early promoter Peter Gruss, Ravi Dhar, and George 943-947 (deletion mutantslchromatin/RNA initiation/transcriptional regulation) Khoury

BIOPHYSICS

Bacterial bioluminescence: Spectral study of the emitters in the in vitro reaction I. B. C. Matheson, John Lee, and 948-952 (low-temperature fluorescence/fluorescent intermediates) Franz Muller

Voltage-dependent inactivation of a calcium channel (calcium currents/ Aaron P. Fox 953-956 inactivation/egg cell membrane)

Energies and kinetics of radical pairs involving bacteriochlorophyll and Vladimir A. Shuvalov and William 957-961 bacteriopheophytin in bacterial reaction centers (photosynthesis/electron W. Parson transfer/spin exchange interactions)

Continuous fluorescence microphotolysis: A sensitive method for study of Reiner Peters, Axel Brunger, and 962-966 diffusion processes in single cells (diffusion equation/lateral diffusion in Klaus Schulten membranes/photobleaching/sea urchin eggs)

5N- and 2H-substituted maleimide spin labels: Improved sensitivity and A. H. Beth, S. D. Venkataramu, K. 967-971 resolution for biological EPR studies (glyceraldehyde-3-phosphate Balasubramanian, B. H. Robinson, dehydrogenase/spin label synthesis/saturation transfer electron paramagnetic L. R. Dalton, D. E. Pearson, resonance/spectral simulation) Charles R. Park, and Jane H. Park

Stochastic flows in integral and fractal dimensions and morphogenesis (lattice Michael D. Hatlee and John J. Kozak 972-975 models/Monte Carlo simulation/reduction of dimensionality)

BOTANY

Mathematical analysis of the chemosmotic polar diffusion of auxin through plant Mary Helen M. Goldsmith, Timothy 976-980 tissues (indoleacetic acid/polar transport of auxin/cellular polarity) H. Goldsmith, and Monroe H.

Martin

Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes Klaus Pfister, Katherine E. 981-985 (triazines/photosystem II/electron transport inhibitors/diuron) Steinback, Gary Gardner, and,

Charles J. Arntzen

CELL BIOLOGY

Transport of sodium, chloride, and taurocholate by cultured rat hepatocytes Bruce F. Scharschmidt and Jeffery E. 986-990 (sodium-coupled transport/bile acid) Stephens

Ca2"- and calmodulin-stimulated endogenous phosphorylation of neurotubulin Briant E. Burke and Robert J. 991-995 (phosphotubulin/tubulin phosphorylation/tubulin kinase/synaptic tubulin/ DeLorenzo brain calmodulin)

Regulation of intracellular levels of calmodulin and tubulin in normal and James G. Chafouleas, Robert L. 996-1000 transformed cells (calcium/calcium-binding proteins/cytoskeleton) Pardue, B. R. Brinkley, John R.

Dedman, and Anthony R. Means

Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/ John E. Repine, Oswald W. 1001-1003 hydrogen peroxide-generated hydroxyl radical (supercoiled DNA/oxygen Pfenninger, David W. Talmage, radicals/radiochemistry/thiourea) Elaine M. Berger, and David E.

Pettijohn Two growth factors and two hormones regulate initiation of DNA synthesis in Luis Jimenez de Asua, K. M. Veronica 1004-1008

cultured mouse cells through different pathways of events (prostaglandin F2] Richmond, and Angela M. Otto epidermal growth factor/insulin/lag phase/regulatory steps)

Blue-light receptor in a white mutant of Physarum polycephalum mediates Thomas Schreckenbach, Barbl 1009-1013 inhibition.of spherulation and regulation of glucose metabolism Walckhoff, and Cornelia Verfuerth (differentiation/photomorphogenesis/fungal polysaccharide/slime mold)

Transfer of synaptic vesicle antigens to the presynaptic plasma membrane during Randall J. von Wedel, Steven S. 1014-1018 exocytosis (immunofluorescence/nerve terminals/neuromuscular junction/ Carlson, and Regis B. Kelly lanthanum/f(3-bungarotoxin)

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Contents

L-Fucose-terminated glycoconjugates are recognized by pinocytosis receptors on Virginia L. Shepherd, Y. C. Lee, Paul 1019-1022 macrophages (mannose-fucose lectin) H. Schlesinger, and Philip D. Stahl

a-Globin sequences are located in a region of early-replicating DNA in murine Allen Furst, Eric H. Brown, Joseph D. 1023-1027 erythroleukemia cells (cell cycle/elutriation/Friend cells/cell synchrony) Braunstein, and Carl L. Schildkraut

Evidence for a higher molecular weight precursor of cholesterol side-chain- Raymond N. DuBois, Evan R. Simpson, 1028-1032 cleavage cytochrome P-450 and induction of mitochondrial and cytosolic Janette Tuckey, J. David Lambeth, proteins by corticotropin in adult bovine adrenal cells (cell culture/cortisol and Michael R. Waterman production/protein radiolabeling/in vitro translation)

erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that P. Bouchard, S. M. Penningroth, A. 1033-1036 blocks dynein ATPase and protein carboxylmethylase activities (axonemal Cheung, C. Gagnon, and C. W. movement/dynein arms/demembranated spermatozoa) Bardin

Calcium lability of cytoplasmic microtubules and its modulation by microtubule- Manfred Schliwa, Ursula Euteneuer, 1037-1041 associated proteins (detergent extraction/calmodulin/calmodulin inhibitors/ Jeanette Chloe Bulinski, and immunofluorescence microscopy) Jonathan G. Izant

Saccharin and cyclamate inhibit binding of epidermal growth factor (tumor L. S. Lee 1042-1046 promotion/phorbol esters/hormone response control unit/carcinogenesis)

Enzymatic hydrolysis of cellulose: Visual characterization of the process Alan R. White and R. Malcolm Brown, 1047-1051 (Trichoderma reesei/Acetobacter xylinum/high-resolution electron microscopy/ Jr. endoglucanase/cellobiohydrolase)

Biosynthetic labeling of insulin receptor: Studies of subunits in cultured human Emmanuel Van Obberghen, Masato 1052-1056 IM-9 lymphocytes (hormone receptor biosynthesis/autoantibodies against Kasuga, Alphonse-Le Cam, Jose A. insulin receptors/down regulation/glycosidases) Hedo, Ahuva Itin, and Len C.

Harrison

Reduction of K+ efflux in cultured mouse fibroblasts, by mutation or by diuretics, David W. Jayme, Edward A. Adelberg, 1057-1061 permits growth in K+-deficient medium (furosemide/cotransport/mutant/ and Carolyn W. Slayman bumetanide/ouabain-insensitive)

Alterations in polyamine levels induced by phorbol diesters and other agents that Eliezer Huberman, Charles Weeks, 1062-1066 promote differentiation in human promyelocytic leukemia cells (tumor Adria Herrmann, Michael Callaham, promoters/putrescine/retinoic acid/anthralin/ornithine decarboxylase) and Thomas Slaga

A cellular protein that associates with the transforming protein of Rous sarcoma Hermann Oppermann, Warren 1067-1071 virus is also a heat-shock protein (src/sodium arsenite/neoplastic Levinson, and J. Michael Bishop transformation/protein kinase)

Inhibition of the mobility of mouse lymphocyte surface immunoglobulins by Yoav I. Henis and Elliot L. Elson 1072-1076 locally bound concanavalin A (cytoskeleton/anchorage modulation/ concanavalin A-platelets/mouse B lymphocytes/photobleaching recovery)

DEVELOPMENTAL BIOLOGY

Isolation and characterization of mouse mutant embryonal carcinoma cells which Joel Schindler, Klaus I. Matthaei, and 1077-1080 fail to differentiate in response to retinoic acid (teratocarcinomasl Michael I. Sherman dif(RA)-mutations/cytoplasmic retinoic acid-binding protein/cytoplasmic retinol-binding protein)

EVOLUTION

Coevolution of species in competition: A theoretical study (density-dependent C. Matessi and S. D. Jayakar 1081-1084 selection/frequency-dependent selection/species packing/character displacement/evolutionary optimization)

Simple method for constructing phylogenetic trees from distance matrices (matrix Wen-Hsiung Li 1085-1089 method/unequal rates of evolution)

GENETICS

A mechanism of DNA transposition (bacteriophage Mu/transposable genetic Rasika M. Harshey and Ahmad I. 1090-1094 elements/integrative replication/DNA amplification/chromosomal Bukhari translocations)

engrailed: A gene controlling compartment and segment formation in Drosophila Thomas Kornberg 1095-1099 (embryogenesis/lethal mutation)

Isolation and characterization of polyoma host range mutants that replicate in Kenji Sekikawa and Arnold J. Levine 1100-1104 nullipotential embryonal carcinoma cells (viral early gene regulation/viral DNA 'duplication near origin/developmental control/DNA sequence)

Structural brain mutant of Drosophila melanogaster with reduced cell number in K. F. Fischbach and M. HIeisenberg 1105-1109 the medulla cortex and with normal optomotor yaw response (cell degeneration/developmental plasticity/orientation behazvior/Golgi staining/ genetic mosaics)

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Contents

Nonrandom patterns of codon usage and of nucleotide substitutions in human, a- G. Modiano, G. Battistuzzi, and A. G. 1110-1114 and /3-globin genes: An evolutionary strategy reducing the rate of mutations Motulsky with drastic effects? (genetic code/evolution/hemoglobin/mutation rates)

Evidence that ribosomal protein S10 participates in control of transcription D. I. Friedman, A. T. Schauer, M. R. 1115-1118 termination (nusE gene/p protein/A N gene product/antiterminationl Baumann, L. S. Baron, and S. L. translation) Adhya

Cell cycle-dependent regulation of thymidine kinase activity introduced into Carl A. Schlosser, Carolyn Steglich, 1119-1123 mouse LMTK- cells by DNA and chromatin-mediated gene transfer Jeffrey R. deWet, and Immo E. (transgenome/gene expression/synchronized cells) Scheffler

Detection of enzyme polymorphism by using monoclonal antibodies (alkaline C. A. Slaughter, M. C. Coseo, M. P. 1124-1128 phosphatase/placenta/allelic variants) Cancro,.and Harry Harris

Some calculations on the amount of selfish DNA (molecular evolution/junk DNA/ Tomoko Ohta and Motoo Kimura 1129-1132 transposable element/repetitive sequence)

IMMUNOLOGY

T-Cell lymphoma model for the analysis of interleukin 1-mediated T-cell Steven Gillis and Steven B. Mizel 1133-1137 activation (T-cell growth factor/lymphocyte-activating factor/interleukins 1 and 2/T-cell differentiation/lymphoma)

Switch from hapten-specific immunoglobulin M to immunoglobulin D secretion in Michael S. Neuberger and Klaus 1138-1142 a hybrid mouse cell line (immunoglobulin genes/fluorescence-activated cell. Rajewsky sorting)

Genetic and serological analysis of the expression of crossreactive idiotypic Man-Sun Sy, Alan Brown, Bruce. A. 1143-1147 determinants on anti-p-azobenzenarsonate antibodies and p- Bach, Baruj Benacerraf, Paul D. azobenzenarsonate-specific. suppressor T cell factors (suppressor factor/ Gottlieb, Alfred Nisonoff, and Mark idiotype/heavy chain VH gene/light chain VK gene/Lyt-3.1 locus) I. Greene

Effects of Lyt antibodies on T-cell functions: Augmentation by anti-Lyt-1 as Nurit. Hollander, Eric Pillemer, and 1148-1151 opposed to inhibition by anti-Lyt-2 .(mixed lymphocyte reaction/T-cell receptor/ Irving L. Weissman T-cell growth factors)

Modulation of Schistosoma mansoni egg-induced granuloma formation: I-J W. F. Green and D. G. Colley 1152-1156 restriction of T cell-mediated suppression in a chronic parasitic infection (suppressor T lymphocytes/immune regulation/major histocompatibility complexlcongeneic mice)

Multiple biologic activities of a cloned inducer T-cell population (inducer T-cell Gary Nabel, Joel S. Greenberger, Mary 1157-1161 clones/T-cell factors/granulocyte-macrophage colony-stimulating factors) Ann Sakakeeny, and Harvey Cantor

Complete amino acid sequence of bovine thymosin 34: A thymic hormone that Teresa L. K. Low, Shu-Kuang Hu, and 1162-1166 induces terminal deoxynucleotidyl transferase activity in thymocyte Allan L. Goldstein populations (differentiation of thymus-dependent-lymphocyte/DNA nucleotidylexotransferase/acetylserine NH2-terminal polypeptide)

Two allelic forms of mouse /32-microglobulin (major histocompatibility antigens/ Peter J. Robinson, Lynn Graf, and 1167-1170 genetic polymorphism) Karin Sege

MEDICAL SCIENCES

Influence of anti-mouse interferon serum on the growth and metastasis of tumor Lola M. Reid, Nagahiro Minato, Ion 1171-1175 cells persistently infected with virus and of human prostatic tumors in Gresser, John Holland, Anna athymic nude mice (natural killer cells/tumor rejection) Kadish, and Barry R. Bloom

Inhibition of tumor growth, vascularization, and collagenolysis in the rabbit Jerome Gross, Richard G. Azizkhan, 1176-1180 cornea by medroxyprogesterone (invasion/angiogenesis/collagenase/steroid Chitra Biswas, Romaine R. Bruns, hormones) Dean S. T. Hsieh, and Judah

Folkman

Transforming activity of human tumor DNAs (transfection/cellular transforming Theodore G. Krontiris and Geoffrey M. 1181-1184 genes/bladder carcinomas) Cooper

N-Nitroso-N-methylurea elicits mammary cancer in resistant and sensitive rat Charles B. Huggins, Norifumi Ueda, 1185-1188 strains. (Long-Evans strain/Sprague-Dawley strain/N-nitroso-N-methylurea and M. Wiessler synthesis/7,8,12-trimethylbenz[a]anthracene)

Specific receptors for phorbol esters in lymphoid cell populations: Role in Julianne J. Sando, Mary L. Hilfiker, 1189-1193 enhanced production of T-cell growth factor (tumor promoters/human David S. Salomon, and John J. lymphocytes/interleukin 2) Farrar

Regulatory role for hepatic low density lipoprotein receptors in vivo in the dog Petri T. Kovanen, David. W. Bilheimer, 1194-1198 (cholesterol synthesis.inhibitor/mevinolin/bile acid sequestrant/colestipol/ Joseph L. Goldstein, John J. catabolism of plasma lipoproteins) Jaramillo, and Michael S. Brown

Localizati.on of 25-hydroxyvitamin D3 la-hydroxylase and 24-hydroxylase along Hiroyuki Kawashima, Shozo Torikai, 1199-1203 the rat nephron (lat,25-dihydroxyvitamin.D3/24,25;-dihydroxyvitamin D3/ and Kiyoshi Kurokawa parathyroid hormone/proximal convoluted tubules/proximal straight tubules)

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A human neutrophil-dependent pathway for generation of angiotensin II: Bruce U. Wintroub, Lloyd B. 1204-1208 Purification and physicochemical characterization of the plasma protein Klickstein, Carol E. Kaempfer, and substrate (angiotensinogen/neutrophil enzymes) K. Frank Austen

Immunohistological demonstration of respiratory syncytial virus antigens in Barbara G. Mills, Frederick R. Singer, 1209-1213 Paget disease of bone (slow virus infection/immunoperoxidase staining! Leslie P. Weiner, and Patricia A. immunofluorescent staining/bone cells) Holst

Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies Jack R. Wands, Rolf I. Carlson, Hubert 1214-1218 (radioimmunoassay/hepatitis B surface antigen!hybridomas) Schoemaker, Kurt J. Isselbacher,

and Vincent R. Zurawski, Jr. Selective killing of human malignant cell lines deficient in methylthioadenosine Naoyuki Kamatani, Walter A. Nelson- 1219-1223

phosphorylase, a purine metabolic enzyme (enzyme deficiency/polyaminesl Rees, and Dennis A. Carson methotrexate/cancer chemotherapy)

MICROBIOLOGY

Cytoplasmic and membrane proteins of yersiniae cultivated under conditions Susan C. Straley and Robert R. 1224-1228 simulating mammalian intracellular environment (virulence antigens! Brubaker restriction/membrane separation)

Effects of chaotropic and antichaotropic agents on elution of poliovirus adsorbed Samuel R. Farrah, Dinesh 0. Shah, 1229-1232 on membrane filters (virus/surfactant/hydrophobic interactions/electrostatic and Lonnie 0. Ingram interactions)

Effects of amphotericin B, nystatin, and other polyene antibiotics on chitin Dora M. Rast and Salomon Bartnicki- 1233-1236 synthase (Mucor rouxii/chitosomes/growth) Garcia

NEUROBIOLOGY

a-Adrenergic antagonists as possible calcium channel inhibitors (a, -adrenergic Daphne Atlas and Michael Adler 1237-1241 receptors/neuroblastoma-glioma hybrid cells/Ca2' antagonistslverapamil)

Net uptake of y-aminobutyric acid by a high-affinity system of rat brain Anna Pastuszko, David F. Wilson, and 1242-1244 synaptosomes (neurotransmitter uptake/high-affinity uptake of 'y-aminobutyric Maria Erecinska acid/neuronal transmission)

Na'-channel-associated scorpion toxin receptor sites as probes for neuronal Yoheved Berwald-Netter, Nicole 1245-1249 evolution in vivo and in vitro (scorpion toxin binding affinity/central nervous Martin-Moutot, Annette Koulakoff, system neurons and glia/cell cultures/differentiation/tetanus toxin binding and Frangois Couraud cells)

Specific monosaccharide inhibition of active sodium channels in neuroblastoma Maria Y. Giovanni, Daniel Kessel, and 1250-1254 cells (high molecular weight glycoprotein/L-fucose/D-galactose/neuraminidase/ Mary Catherine Glick excitable membranes)

Enkephalin-like immunoreactivity of olivocochlear nerve fibers in cochlea of Jorgen Fex and Richard A. Altschuler 1255-1259 guinea pig and cat (opioid/cholinergic neurons/organ of Corti/ immunofluorescence/efferent synapses)

Dynorphin immunocytochemical localization in brain and peripheral nervous S. J. Watson, H. Akil, Vartan E. 1260-1263 system: Preliminary studies (opioid peptides/endorphins/anatomy/ Ghazarossian, and Avram Goldstein immunohistochemistry)

Target neuron-specific process formation by embryonic mesencephalic dopamine L. M. Hemmendinger, B. B. Garber, P. 1264-1268 neurons in vitro (neuronal recognition/fluorescence histochemistry/aggregating C. Hoffmann, and A. Heller cell cultures/catecholamine neurons)

Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma E. Edward Baetge, Barry B. Kaplan, 1269-1273 cells is enhanced by dexamethasone (PC 12 cells/enzyme induction/ Donald J. Reis, and Tong H. Joh glucocorticoids/immunoprecipitation/protein synthesis)

Identification and kinetic properties of the current through a single Na+ channel Yasuo Fukushima 1274-1277 (patch recording/open-close kinetics/tunicate egg)

Membrane lipid heterogeneity associated with acetylcholine receptor particle Paul C. Bridgman and Yasuko 1278-1282 aggregates in Xenopus embryonic muscle cells (cholesterol/filipin/saponin/ Nakajima synaptogenesis/freeze-fracture)

Requirement for nonoligodendrocyte cell signals for enhanced myelinogenic gene Shama Bhat, Elisa Barbarese, and S. 1283-1287 expression in long-term cultures of purified rat oligodendrocytes (primary E. Pfeiffer culture/myelin basic protein/galactolipids/2',3'-cyclic-nucleotide 3'- phosphodiesterase/cellular interaction)

Cell surface modulation of gene expression in brain cells by down regulation of James F. McGinnis and Jean de Vellis 1288-1292 glucocorticoid receptors (lectins/induction of glycerol phosphate dehydrogenase/C6 rat glioma cells/oligodendrocytes/astrocytes)

Twro calcium/calmodulin-dependent protein kinases, which are highly Mary B. Kennedy and Paul Greengard 1293-1297 concentrated in brain, phosphorylate protein I at distinct sites (synaptic vesicles/phosphoproteins)

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Contents

POPULATION BIOLOGY

A multilocus estimator of mating system parameters in plant populations (mixed D. V. Shaw, A. L. Kahler, and R. W. 1298-1302 mating model/self-fertilization/allozyme markers) Allard

Trade-off between r-selection and K-selection in Drosophila populations Laurence D. Mueller and Francisco J. 1303-1305 (population dynamics/density-dependent selection/evolution/Drosophila Ayala melanogaster)

Sex choice and the size advantage model in jack-in-the-pulpit (Arisaema David Policansky 1306-1308 triphyllum) (sex change/reproductive success/demography/protandry)

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INFORMATION TO CONTRIBUTORS

(Revised 1981)

Purpose and scope

The Proceedings of the National Academy of Sciences USA publishes reports that describe the results of original theo- retical or experimental research of exceptional importance or novelty. Reports are accepted only if contributed by a member or foreign associate of the Academy or communi- cated by an Academy member or foreign associate on behalf of a nonmember and if they are not being submitted for pub- lication or have not been published elsewhere. Communi- cated papers should be of a high scientific quality such as to place them in the top 10th percentile of their field.

Because the PROCEEDINGS publishes papers in all branches of science represented in the Academy, articles should be of interest and intelligible to a broad scientific audience.

Occasionally, preliminary findings of major importance may be published in the PROCEEDINGS without full docu- mentation; in such cases, the author must indicate the pre- liminary character of the paper and where a subsequent de- tailed report will be published.

Upon invitation from the Editorial Board, papers pre- sented at Academy symposia, special Academy lectures, re- views, or papers on subjects of general interest to scientists are also published in the PROCEEDINGS.

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Manuscripts should be sent, in duplicate, to the PROCEED-

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Before communicating an article on behalf of nonmem- ber(s), an Academy member must obtain signed written opinions of the paper from two qualified referees. Only the member should select the referees; their names need not be divulged to the author. Referees should be chosen solely for their impartiality and their ability to judge the work. Forms for the referees' comments are provided to members on re- quest to the PROCEEDINGS Office. An author should see all referee comments before the paper is forwarded to the PRO- CEEDINGS Office. Signed copies of both referee reports must accompany the manuscript. The member may communicate a paper for which a referee report is unfavorable, but the rea- sons for so doing should be stated.

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Manuscripts that report research involving either human subjects or the use of materials of human origin must be ac- companied by a copy of the document authorizing the pro- posed research, issued and signed by the appropriate offi- cial(s) of the institution where the work was conducted. Authors are referred to the Declaration of Helsinki for fur- ther guidance.

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Length. Articles should be as brief as full documentation allows. They may not exceed five printed pages (approxi- mately 5000 words). (Review articles may be up to eight pages.) The title, key terms, names of authors and their af- filiation(s), and statement of communication or contribution usually occupy space equal to about 250 words. Appropriate allowance must be made for the space occupied by footnotes, references, tables, and figures with their legends. Final size of figures is decided in the PROCEEDINGS Office to make the best use of space; authors' suggestions will be considered (see Illustrations, below). Instructions for calculating the length of a manuscript are available from the PROCEEDINGS Office. However, responsibility for final measurements of manuscript length is retained by the PROCEEDINGS Office.

Articles estimated to exceed five pages will be returned for shortening, without further editorial consideration.

Title Page. Provide a title page containing only the follow- ing information:

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Classification. Give any one of the sciences named in the titles of the Academy Sections with these exceptions: Sec- tions 41 (Medical Genetics, Hematology, and Oncology), 42 (Medical Physiology, Endocrinology, and Metabolism), and 43 (Infectious Diseases and Immunology) are all listed as Medical Sciences; and Section 27 (Population Biology, Evo- lution, and Ecology) may be listed as Population Biology, Evolution, or Ecology. Additional headings that may be used but that are not Academy Sections are Biophysics, Immu- nology, Microbiology, and Statistics.

Title. The title should be brief, specific, and rich in in- formative words. Titles usually should not begin with such general words as "The," "A," "Results, " "Study,'" or "Ef- fect. "

Titles should not include phrases in which more than three words modify another word (e.g., "simian virus trans- formed fetal mammalian heart fibroblast" should be written as "simian virus-transformed fibroblast from fetal mamma- lian heart"). The PROCEEDINGS does not use serial titles such as On Steroids, XIX. A serial title followed by a colon and the specific title may be used (e.g., On steroids: Metabolism of cortisone). If a paper is part of a series, this may be indicated by a symbol at the end of the title and a footnote: "This is paper no. 19 in a series. Paper no. 18 is ref. . " The pre- ceding paper must then be included in the list of references.

Key Terms. These terms should be given below the title, enclosed within parentheses, and separated by a slash (/) mark. Up to five terms, composed of words not used in the title, may be included to alert readers and indexers to other subjects in the paper.

Author Affiliation. The department, institution, city, state, and ZIP code or country for each author should be fur- nished. If there are several authors with different affilia- tions, authors should be matched to their respective institu- tions by means of superscript symbols after the authors' names in this order: *, t7 f, ?, , 11.

If an author has moved since doing the work described, indicate the institution at which the work was done in the primary affiliation listing and use an additional symbol and footnote to indicate the present address.

If reprint requests are to be addressed to an author other than the first one listed, also indicate this with a symbol as noted above and a footnote. The footnote should state: "To whom reprint requests should be addressed."

Abbreviations Footnote. Nonstandard abbreviations that are usedfive or more times throughout the paper should be listed in this section. The PROCEEDINGS discourages the use of acronyms, and manuscripts will be edited so that abbre- viations are consistent with recommended international no- menclature. Nonstandard abbreviations should be kept to a minimum and should not be used in the title, key terms, or abstract. Introduce nonstandard abbreviations by defining where first mentioned in the text followed by the abbrevia- tion in parentheses. Subsequently, the abbreviation only should be given.

Abstract Page. The second page of every paper must carry only the abstract. The abstract should be no longer than 250 words. It should state the subject and main conclusions of the article in generally intelligible terms. Abbreviations should be avoided unless used more than five times in the abstract. The abstract must be understandable to the reader before he reads the paper, suitable for reproduction without rewriting, and unambiguous without recourse to any other part of the manuscript.

Footnotes in Text. The number of footnotes should be kept to a minimum and they should be indicated in text by use of a symbol. Symbols should be used in the order *~, t, t, ?, ?, 1I, but should not repeat symbols used earlier, partic- ularly those of the title page.

Acknowledgments. All acknowledgments, including those for financial support, should be cited here rather than in footnotes. Acknowledgments to people usually precede

those for grant support. Names of grant sources should be spelled out.

References. Cite references in numerical order as they appear in the text. Tables and figures will be inserted in the text where first cited, so references in these sections should be numbered accordingly. Use Arabic numerals separated by commas (except for sequences of three or more when a dash is used between the first and last numeral) and enclose the references in parentheses. References may not be cited in any other order.

Only papers that are either published or in press may be cited in the reference list. Each reference is given a separate number; multiple citations under one numeral are not used. Other citations are either "unpublished data" or "personal communication" and are shown directly in the text where first mentioned.

The full citation is given at the end of the manuscript. The PROCEEDINGS requires inclusive pagination. If a reference is an abstract, then this should be noted in the citation, after the page numbers.

Journal articles are cited as follows: 10. Smith, A. B., Jones, C. D. & Robinson, E. F. (1969)

Proc. Natl. Acad. Sci. USA 64, 191-195. For the correct abbreviations of journal titles, refer to Chem- ical Abstracts Service Source Index (CASSI) and Biblio- graphic Guidefor Editors andAuthors (1974). Both are pub- lished by the American Chemical Society, Washington, DC.

Articles or chapters in books are cited as follows: 11. Jones, C. D. & Shapiro, L. M. (1966) in Enzyme Reac-

tions in Protozoa, eds. Smith, T. G. & Williams, H. I. (Universal Press, New Brunswick, NJ), Vol. 21, pp. 646-672.

Authors are responsible for the accuracy of the citations. Tables. Tables should be prepared so that they are self-ex-

planatory. Vertical rules are not used to set columns apart, and horizontal rules are used sparingly. Tables should have a brief title and be numbered with Arabic numerals. Each table should be on a separate page and be typed double- spaced throughout. Nonstandard abbreviations should be used sparingly and must be defined in the legend at the bot- tom of the table, if not defined in the abbreviations section on the title page. Each vertical column requires a heading. Reference to footnotes should be made by means of the sym- bols *, t, t, ?, ?, 11, in that order. If more than six footnotes are required, then superscript lower-case letters should be used. For use of powers of 10, see section below.

Illustrations. Either original drawings or high-quality photographs are needed. Xerographic copies of illustrations are not acceptable. When illustrations are reduced for pub- lication, they should (i) fit into a one-column (83 mm) or two- column (175 mm) width and (ii) have letters no smaller than 1.5 mm in height. All illustrations should be identified on the reverse side by marking with a soft pencil. Where nec- essary, the orientation for the illustration should be indicated by an arrow and the word "top. " Legends for the illustrations should be typed double-spaced, in numerical order, on a separate page.

Line drawings should be marked with index lines and la- beled with scales on the x- and y-axes. Scales that involve large or small numbers should use numbers multiplied by powers of 10. The PROCEEDINGS uses the following conven- tion: 3000 cpm is represented as 3 and the axis is labeled cpm x 10-3. For maximal clarity, use both separate symbols and various lines (solid, broken, etc.). Keys to symbols and lines should be included in the legend, not on the figure. It is par- ticularly important that nomenclature, abbreviations, and units used in figures agree precisely with those used in the text of the paper. For electron micrographs and similar illus- trative materials, the quality of reproduction cannot exceed the quality of the submitted copy. Do not mount illustrations unless a composite figure is required. A composite figure carries a single figure number; the individual parts may be

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identified by letters. Indicate the magnification of photomi- crographs in the legend or include a bar in the figure to in- dicate the scale (or both).

Abbreviations and Symbols. The PROCEEDINGS distin- guishes between standard and nonstandard abbreviations. Standard abbreviations for certain substances and for units of measure do not need to be defined. Most other abbrevia- tions are considered to be nonstandard, should be kept to a minimum, and must be spelled out on first usage. Nonstand- ard abbreviations should be used only for terms mentioned five or more times in the paper (see Title Page), should be unambiguous, and should not simply be acronyms. Both standard and nonstandard abbreviations should also conform to international standards. Authors should refer to: IUPAC

Manual of Symbols and Terminology for Physicochemical Quantities and Units (1970) Butterworths, London, En- gland; National Bureau of Standards Special Publication 330 (1977), (United States Government Printing Office); or Pure and Applied Chemistry (1970) 21, 3-44.

Nomenclature. International standards on nomenclature should be used. The following guides are recommended:

Mathematics. A Manualfor Authors of Mathematical Pa- pers (1970). American Mathematical Society, 321 South Main Street, P.O. Box 6248, Providence, RI 02904.

Provide a list of special characters used in the paper for the printer, and identify Greek, Hebrew, or script letters in the margin at the line where they first appear.

Physics. Style Manualfor Guidance in the Preparation of

Some Standard Abbreviations

absorbancea A acetate OAc acetyl Ac N-acetylglucosamine GlcNAc adenine Ade adenosine b,c A, Ado adenosine 3' :5'-cyclic monophosphate cyclic AMP alanined Ala arginined Arg asparagined Asn asparagine or aspartic acidd Asx aspartic acidd Asp 5-bromodeoxyuridine BrdUrd carbon monoxide hemoglobin HbCO carbon monoxide myoglobin MbCO O-carboxymethylcellulose CM-cellulose coenzyme. A CoA complementary DNA cDNA counts per minute cpm cysteine or '/2 cystined Cys cytidinebc C, Cyd cytosine Cyt deoxy (carbohydrates and nucleotides) d de.oxyribonuclease DNase diethylaminoethylcellulose DEAE-cellulose electron spin resonance ESR flavin-adenine dinucleotide FAD flavin mononucleotide FMN formylmethionined fMet fructose Fru fucose Fuc galactosamine GalN galactose. Gal gluconic acid GlcA glucosamine GlcN glucosee G, Glc glucuronic acid GlcUA glutamic acidd Glu glutamic acid or glutamined Glx glutamined Gln glycined Gly guanine Gua

guanosine b,c G, Guo hemoglobin Hb histidined His immunoglobulin Ig (IgG, IgM, etc.) inosineb C I, Ino isoleucined Ile isotopesf 14C, 3H, 32P leucined Leu lysined Lys mannose Man messenger RNA mRNA methionine d Met mitochondrial DNA mtDNA myoglobin Mb nicotinamide-adenine dinucleotide NAD+, NADH nicotinamide-adenine dinucleotide

phosphate NADP+, NADPH nicotinamide mononucleotide NMN nuclear magnetic resonance NMR nucleoside (unknown)bc, N, Nuc optical densitya OD oxyhemoglobin HbO2 phenylalanined Phe phosphate (in compounds) P or p phosphate (inorganic) Pi poly(adenylic acid)" poly(A) prolined Pro ribonuclease RNase ribose Rib ribosomal ribonucleic acid rRNA ribosylthymine T or Thd serine d Ser sodium dodecyl sulfate. NaDodSO4 threonined Thr thymidine (2t-deoxyribosylthymine)b,C dT or dThd thymine Thy transfer RNA tRNA tryptophand Trp tyrosined Tyr uracil Ura uridinelbc U, Urd valined Val

a Use absorbance, A, for light absorption by solutions; use op- tical density, OD, for light transmission through turbid sus- pensions.

b The 5' mono-, di-., and triphosphate derivatives are abbre- viated as AMP, ADP,. ATP; other nucleoside .5'-phosphates are abbreviated similarly.

'Use in abbreviations for substituted purines, pyrimidines, and nucleosides..

d Use only in. sequence descriptions, tables, or figures. eUse three-letter abbreviation when single letter is ambig-

uous.

'The mass number is written as a superior prefix: 14C, not C14. Enclose the isotope symbol in square brackets imme- diately. before the name or abbreviation of the compound: [32P]CMP; ['4C]urea; [1-_4C] leucine; L-[methyl- 14C]methionine. Do not use brackets if the atom does not occur naturally in the compound or if the isotope is not used in a specific sense. Examples are [.'251]thyroxine but 25'I-la- beled trypsin; [U-3H]arginine but 3H-labeled amino acids. With chemical formulas, use without brackets as. 14CO2, H232SO4.

g Similarly abbreviate oligo- and polynucleotides containing the purine and pyrimidine bases listed in this table.

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Abbreviations for Units of Measurement and of Physical and Chemical Quantities

Prefixes to the Names of Units exa 1018 E deci lo-0 d peta 10'5 p centi 10-2 c

tera lo12 T milli i0-3 m giga 109 G micro 10-6 A mega 106 M nano 10-9 n kilo 103 k pico lo-12 p

femto lo-15 f atto lo-18 a

Units of Concentration* molar (mol/liter) M parts per million ppm

Units of Length meter m micrometer (not micron) ,um (not ,u) nanometer nm (not m,u) angstrom (0.1 nm) A

Units of Volumet milliliter ml microliter ,ul (not A)

Units of Mass gram g microgram ,uLg (not 'y)

Units of Time hour hr year yr minute min month mo second s, sec week wk

Units of Electricity ampere (milliampere) A (mA) ohm siemens (mho) S volt V

Units of Energy and Work joule J calorie cal

Units, of Temperature degree Celsius thermodynamic temperature (kelvin) K

Units of Radioactivity counts per minute cpm curie(s) Cit disintegrations per minute dpm

Miscellaneous Units candela cd cycles per second (hertz) Hz lumen lm lux lx pascal (newton/meter2) Pa revolutions per minute rpm Svedberg unit of sedimentation

coefficient (10-13 s) S

Physical and Chemical Quantities retardation factor RF

acceleration of gravity g partial specific volume v maximum velocity Vmax

sedimentation coefficient s equilibrium constant K Michaelis constant Km molecular weight Mr rate constant k

Other Words standard deviation of series SD standard error of mean SEM probability P

The PROCEEDINGS uses units that are part of the Systeme International (SI). When such units are not used, the SI equivalent should be given in parentheses where first mentioned. * For units of concentration based on molecular weight, the mole is used. Weight concentrations should be given as g/ml,

g/liter, ppm, etc.; mg% is not acceptable. f Liter should not be abbreviated unless used with a prefix. t In SI, 1 Ci = 3.7 x 1010 becquerels (Bq); therefore, 1 Bq = 60 dpm.

Papers Published by the American Institute of Physics (1973). American Institute of Physics, 335 East 45th Street, New York, NY 10017.

Chemistry. Handbook for Authors of Papers in, the jour- nals of the American Chemical Society (1978). American Chemical Society Publications, 1155 16th Street, N.W., Washington, DC 20036.

Psychology. Publication Manual of the.American Psycho- logical Association '(1974). American Psychological Associa- tion, 1200'17th Street, N.W., Washington, DC 20036.

Life Sciences. Council of Biology Editors Style Manual (1978), 4th Ed. American Institute of Biological Sciences, 1401 Wilson Boulevard, Arlington, VA 22209. Biochemical Nomenclature and Related Documents (1978) (a compen- dium of IUPAC-IUB documents, some of which have ap- peared elsewhere); Biochemical Society, P.O. Box 32, Com- merce Way, Colchester, C02'8HP, Essex, England.

The second Life Sciences reference contains the Interna- tional Union of Biochemistry rules of nomenclature for amino acids, peptides, nucleic acids, polynucleotides, vita-

mins, coenzymes, quinones, folic acid and related com- pounds, corrinoids, lipids, enzymes, proteins, cyclitols, ste- roids, carbohydrates, carotenoids, peptide hormones, and human immunoglobulins.

Enzymes should be given the recommended name fol- lowed in parentheses by the systematic name and Enzyme Commission (EC) number on first mention,.in both the ab- stract and text. For guidance refer to: Enzyme Nomencla- ture: Recommendations (1978) of the Nomenclature Com- mittee of the International Union of Biochemistry (1979). Academic Press Inc., New York.

Genetics. A guide to nomenclature in bacterial genetics may be found in: Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966) Genetics 54, 61-76. Note that genotypes are italicized (underlined in typed copy); pheno- types are not.

Immunology. For human immunoglobulins and their ge- netic factors use the rules of the World Health Organization or the first reference for Life Sciences above.

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Missing pages are

Physical Sciences issues.

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