1 Ho There is no significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.Ha Theres significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

2 level of significance 0.05degrees of freedom 16

tabulated value 1.746

3 statistics tool Anova: Single Factor

4 computation t-Test: Two-Sample Assuming Unequal Variances

TABLE# hyperuric # with saluyot

1 11.65 1 10.412 11.34 2 12.353 13.30 3 14.347 12.54 7 10.648 10.22 8 10.189 15.80 9 12.11

10 22.77 10 10.3111 6.61 11 4.3412 10.18 12 8.36

mean difference 2.374444

t-Test: Two-Sample Assuming Equal Variances

hyperuric with saluyott-Test: Two-Sample Assuming Equal Variances

hyperuric with saluyotMean 12.71222 10.33778Variance 20.49252 7.903861Observations 9 9Pooled Variance 14.19819Hypothesized Mean Dif 2.37df 16t Stat 0.002502P(T<=t) one-tail 0.499017t Critical one-tail 1.745884P(T<=t) two-tail 0.998035t Critical two-tail 2.119905

computed value 0.0025

5 DECISION since comp < tabulatedHo AcceptHa Reject

interpretation There is no clinically significant blood uric acid lowering activity of using glycoside extract from saluyot (Corchorus olitorius L.) leaves

There is no significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.Theres significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

There is no clinically significant blood uric acid lowering activity of using glycoside extract from saluyot (Corchorus olitorius L.) leaves

1 Ho There is no significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).Ha There is significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).

2 level of significance 0.05degrees of freedom 5

tabulated value 2.015

3 statistics tool Anova: Single Factor

4 computation t-Test: Two-Sample Assuming Unequal Variances

TABLE# w/ saluyot # positive

1 10.64 1 2.612 10.18 2 5.933 12.11 3 2.314 10.315 4.346 8.36

mean difference 5.704444

t-Test: Two-Sample Assuming Unequal Variances

w/ saluyot positiveMean 9.323333 3.618889Variance 7.399436 4.040493Observations 6 3Hypothesized Mean Dif 5.7df 5t Stat 0.002767P(T<=t) one-tail 0.49895t Critical one-tail 2.015048P(T<=t) two-tail 0.997899t Critical two-tail 2.570582

computed value 0.00277

5 DECISION since comp < tabulated

Ho AcceptHa Reject

interpretation There is no significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).

There is no significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).There is significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).

There is no significant difference between the blood uric acid lowering activity of saluyot (Corchorus olitorius L.) glycoside and commercially available drug (allopurinol).

1 Ho There is no significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.Ha There isignificant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

2 level of significance 0.5degrees of freedom 7 and 3

tabulated value 3.072467

3 statistics tool Anova: Two-Factor Without Replication

4 computation

TABLEantiserum pure 9:01 8:02 7:03

gross

A 4.000 3.000 3.000 3.000 2.400AB 4.000 1.000 1.000 1.000 1.000B 4.000 2.400 2.000 2.000 2.000O 1.000 1.000 1.000 1.000 1.000

micros

A 5.000 4.000 4.000 4.000 3.600AB 5.000 2.400 2.200 2.200 2.000B 5.000 4.000 4.000 4.000 3.600O 1.000 1.000 1.000 1.000 1.000

Anova: Two-Factor Without Replication

SUMMARY Count Sum Average Variance4 4 11.4 2.85 0.094 4 4 1 04 4 8.4 2.1 0.041 4 4 1 05 4 15.6 3.9 0.045 4 8.8 2.2 0.0266666675 4 15.6 3.9 0.041 4 4 1 0

pure 8 18.8 2.35 1.620.375694444444445 8 18.2 2.275 1.6335714290.334722222222222 8 18.2 2.275 1.633571429

0.29375 8 16.6 2.075 1.176428571

ANOVASource of Variation SS df MS F P-value

Rows 42.06875 7 6.009821429 335.4318937 4.35191E-20Columns 0.33375 3 0.11125 6.209302326 0.003454773Error 0.37625 21 0.017916667

5

Total 42.77875 31

computed value 6.2093023

5 DECISION since comp > tabulatedHo RejectHa Accept

interpretation There isignificant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

There is no significant hemagglutinating effect of lima bean seed extract when tested against red blood cell.There isignificant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

F crit2.4875783.072467

There isignificant hemagglutinating effect of lima bean seed extract when tested against red blood cell.

Table 1. To (Commercial Anti-sera) under Gross observationBS A B AB O AVE

1 4 4 4 1 3.252 4 4 4 1 3.253 4 4 4 1 3.254 4 4 4 1 3.255 4 4 4 1 3.25

Average 4 4 4 1 3.25

Table 2. T1 (Pure Lima Bean Seed Extract) under Gross observationBlood Sam A B AB O

1 3 1 2 1 1.752 3 1 2 1 1.753 3 1 2 1 1.754 3 1 3 1 25 3 1 3 1 2

Average 3 1 2.4 1 1.85

Table 3. T2 (9:1 – Lima Bean Seed Extract : NSS) under Gross observationBlood Sam A B AB O

1 3 1 2 1 1.752 3 1 2 1 1.753 3 1 2 1 1.754 3 1 2 1 1.755 3 1 2 1 1.75

Average 3 1 2 1 1.75

Table 4. T3 (8:2 – Lima Bean Seed Extract: NSS) under Gross observationBlood Sam A B AB O

1 3 1 2 1 1.752 3 1 2 1 1.753 3 1 2 1 1.754 3 1 2 1 1.755 3 1 2 1 1.75

Average 3 1 2 1 1.75

Table 5. T4 (7:3 – Lima Bean Seed Extract:NSS) under Gross observationBlood Sample

A B AB O1 2 1 2 1 1.52 3 1 2 1 1.753 2 1 2 1 1.54 3 1 2 1 1.755 2 1 2 1 1.5

Average 2.4 1 2 1 1.6

Table 6. To (Commercial Anti-sera) under Microscopic observationBlood Sam A B AB O

1 5 5 5 1 42 5 5 5 1 43 5 5 5 1 44 5 5 5 1 45 5 5 5 1 4

Average 5 5 5 1 4

Table 7. T1 (Pure Lima Bean Seed Extract) under Microscopic observationBlood Sam A B AB O

1 4 3 4 1 32 4 2 4 1 2.753 4 3 4 1 34 4 2 4 1 2.755 4 2 4 1 2.75

Average 4 2.4 4 1 2.85

Table 8. T2 (9:1 – Lima Bean Seed Extract:NSS) under Microscopic observationBlood Sam A B AB O

1 4 3 4 1 32 4 2 4 1 2.753 4 2 4 1 2.754 4 2 4 1 2.755 4 2 4 1 2.75

Average 4 2.2 4 1 2.8

Table 9. T3 (8:2 – Lima Bean Seed Extract: NSS) under Microscopic observationBlood Sam A B AB O

1 4 3 4 1 32 4 2 4 1 2.753 4 2 4 1 2.754 4 2 4 1 2.755 4 2 4 1 2.75

Average 4 2.2 4 1 2.8

Table 10. T4 (7:3 – Lima Bean Seed Extract:NSS) under Microscopic observationBlood Sam A B AB O

1 4 2 3 1 2.52 3 2 4 1 2.53 3 2 4 1 2.54 4 2 3 1 2.55 4 2 4 1 2.75

Average 3.6 2 3.6 1 2.55