PharmaTome The souvenir

PharmaTome PharmaTome The souvenir

Evolving Pharmaceutical Regulatory & Quality System Framework

“Pharma Profession have gone to the extent of penetrating the consumer mind withNeuromarketing”

Dr. Vijay BambulkarIPA, President, Mumbai

Dr. C. K. KokateVice- Chancellor,

KLE University, Belgaum, Karanataka

3 & 4 March 2012Special Issue

Patron

Hon. G. D. PatilSecretary, SWVSM

Co- Convenor

Dr. Ujwala S. ChouguleAdmin. Officer

Convenor

Dr. John I. DisouzaPrincipal

Director Regulatory & Medical Affairs,

Johnson & Johnson Limited

I am glad to know about the two-day AICTE sponsored national conference on “Evolving Pharmaceutical Regulatory and Quality System Framework” at TKCP, Warananagar on

rd th3 and 4 March, 2012.Tatyasaheb Kore College of Pharmacy is working under the parent organization, Shri Warana Vibhag Shikshan Mandal, Warananagar and it gives me immense pleasure and pride that a national technical event is held in rural part of India. I am delighted to look at the fast pace of TKCP, which has made a identity of its own in just seven years of establishment. Also, the research funds it receives continuously from AICTE, UGC and Shivaji University, Kolhapur cannot be underestimated. With serene feelings, I congratulate Dr John I Disouza, Principal, TKCP and his faculty members for the efforts and wish them a grand success. An amalgamation of national and international speakers will add much luster to the knowledge of the delegates. I convey them my best wishes.

Hon’ble Shri G. D. PatilSecretary,

Shri Warana Vibhag Shikshan Mandal, Warananagar

Patron’sPatron’sDesk

It gives me great pleasure to place before you, “souvenir and abstract book” scientific proceedings of AICTE sponsored national conference on “Evolving Pharmaceutical Regulatory and Quality System Framework”.Shri Warana Vibhag Shikshan Mandal's Tatyasaheb Kore College of Pharmacy is located in a small town of Warananagar and is booming high with vision and strong support of our Chairman Hon. Vinayraoji Kore and encouragement and guidance of our Secretary Hon. G. D. Patil. Incepted in 2004; with mission to “Excel in Professional Pharmacy Education through Student Centered Learning, Scholarly Research and Service to Society”. It has rapidly made an identity of its own for quality oriented education. Support for research and scientific meets is another facet of TKCP. With this view, AICTE sponsored this National Conference and allowed us to bring many eminent personalities from industry, corporate world and stake holders of pharmaceutical academia together. We are indebted to AICTE for their financial support and also to the various well wishers who helped us, directly or indirectly. Happy reading!!!

Dr. John DisouzaPrincipal

Tatyasaheb Kore College of Pharmacy, Warananagar

ConvenerConvenerDesk

Co-ConvenerCo-ConvenerDesk

I, the co-convener of the organizing committee, have the privilege and pleasure of to be the part of AICTE sponsored two days National Level Conference on “Evolving Pharmaceutical

rd thRegulatory and Quality System Frame work” from 3 & 4 March 2012. We, the local organizing committee, are committed to make this conference a unique one with a variety of exciting high quality programme.I feel pleased and proud to announce that conference is pioneering work in generating quality human resource and undertaking need based research helped in ushering white revolution in India.My abundant blessing and grace, no doubt, I can assure you that, it will be a memorable two days with all grand success.I would like to express Heartfelt thanks to The Hon'ble Secretary G.D. Patil Sir & I congratulate Dr. J .I. Disouza Sir, all other members of the organizing committee, for the herculean task undertaken in hosting this important National Level Conference event at our campus.

rd thI look forward to welcoming all delegates to an energetic and informative on 3 & 4 March 2012 for National Level Conference.

Dr.Ujwala S. ChouguleAdministrative officer, Tatyasaheb Kore College of Pharmacy, Warananagar

EditorialEditorialDesk

I would like to thank organization body to conducting national conference

rd th on 3 and 4 march 2012 at

Warananagar and offering such a marvelous opportunity of souvenir coordinator. During my operational episode I witnessed this grand conference and observed it from all close quarters. This conference has enlighten the bright minds of youth pharmacist and also aspires about achieving its own industrial goal. Many topics under the category 'Pharmatome' are found to be resources available for burgeoning scientists. I hope you will find the information inside to be very helpful and worth talking. The conference provided such a nice platform for creative spawning. There is a huge amount of interesting information foryou to scan over and find the information of your interest.To conclude, I would like to mention that, this abstract book will definitely serve as Lamppost for all of us. The name implies pharma Digest. This abstract book is result of scavenging of all vital moments and information that I witnessed during this magnificent occasion.Hoping a bright future to all of you.Thanking you.

'TATYASAHEB KORE COLLEGE OF PHARMACY, WARANANAGAR 'EVOLVING PHARMACEUTICAL REGULATORY AND QUALITY SYSTEM FRAMEWORK'

'PHARMATOME'

Mr. Kiran Patil Souvenir Coordinator


Speakers

Profile

Speakers

Profile

Speakers Profile

Having worked at esteemed research organizations, Dr. Sanjay Boldhane has huge experience in NDDS, generic product development and IPR. He graduated, post- graduated and obtained doctorate from Shivaji University, Kolhapur. His interests include platform technologies for Oral Controlled Release Systems, Co-crystal techniques, Nano/Lipid Suspenions/Solutions and Metered Dose Transdermal Spray. Presently, he is working as Associate Director at Abbott Healthcare. Earlier, he was associated with Panacea Biotech, Orchid Healthcare, Lupin Ltd. and Almet Corporation. He has 15 patents to his credit and has published 15 papers in various journals and presented 2 papers. He has also worked as BOS member of Shivaji University, Pune University and North Maharashtra University, Jalgaon.

Dr. Vijay Bambulkar

Director Regulatory & Medical Affairs,

Johnson & Johnson LimitedTopic: Regulatory Framework for Fast

Moving Consumer Goods

Speakers Profile

Currently the General Manager (Qualtiy) at GSK Ltd., Mr Kulkarni has worked as Director (Quality) at Baxter Ltd. and Sanofi Aventis. He graduated from Shivaji University and Goa University. Apart from this, he has also worked as Manager-Quality Assurance at Ranbaxy; Manager - Quality Systems & Regulatory Affairs, Indoco Remedies Ltd; Executive-Quality Services, Novartis and Executive- Quality Assurance, Rhone-Poulenc. He has huge experience on QBD and QMS systems.

Mr. Abhijit Kulkarni

General Manager Quality,

GlaxoSmithKline Pharmaceuticals Ltd.

Topic: ICH Q10: Pharmaceutical Quality System

Speakers Profile


Dr. Sanjay Boldhane

Sr. Group Leader, Innovative Drug Delivery Research, Pharmaceutical R & D, Abbott Healthcare Private LimitedTopic: Regulatory requirement for NewProducts [505b(2)]

Speakers Profile


Mr. Shrinivasa Chunekar Deputy Director, Quality Assurance,

Serum Institute of India LimitedTopic: Quality Systems for Vaccines, Sera &

Biological products

Speakers Profile

Dr C S Patil had obtained his doctorate from the prestigious Panjab University, Chandigarh. He is presently Deputy General Manager at Panacea Biotech, Navi Mumbai. In the past he was associated with Nicholas Piramal India Ltd. as a Research Associate.

Dr. Chandrashekhar Patil Deputy General Manager, Pharmacology and Toxicology,

Panacea Biotec LimitedTopic: Pharmaceutical Regulatory Framework in

Preclinical Testing

Conference

Conference

DetailsDetails

AICTE Sponsored

Evolving Pharmaceutical Regulatory & Quality System Framework

National Level Conference on

Q

rd th3 - 4 March, 2012

Venue:Shree Warana Vibhag Shikshan Mandal'sTATYASAHEB KORE COLLEGE OF PHARMACYWarananagar, Tal: Panhala, Dist: Kolhapur, 416 113 (MS)Phone: (02328) 223501, 223526, Fax: 223501; Website: www.tkcpdwarana.orgEmail: [email protected], [email protected]

PatronHon. G. D. Patil

ConvenorDr. John I. Disouza

Co- ConvenorDr. Ujwala S. Chougule

Secretary, SWVSM

Admin. Officer Principal

Mr. V. H. Potdar 9270649855

Co-ordinator Co- Coordinator

Mr. S. A. Payghan9096202858

Mr. A. K. Mullani9423800871

Stage Co-ordination

Mr. M. C. Mahanthesh9850385969

Accommodation Recreation

Mr. S. C. Burli9527003373

Mrs. S. S. Shinde9960892930

Inaugural/valedictory

Souvenir

Mr. K. S. Patil 7798884959

Mr. R. A. Patil 9850594845

Transportation Felicitation

Ms. V. D. Khanvilkar9421150762

Ms. S. R. Patki9881446227

Anchoring Conference kit

Mr. S. D. Chavan 9421204929

Invitation Catering

Mr. C. M. Jamkhandi8805567832

Mr. M. V. Shinde9623159491

Mr. D. A. Bhagwat9561350999

Scientific

Stage Decoration

Mrs. H. A. Mangale8888619950

Ms. S. R. Desai 9890108676

Registration Expert Invitee

Mr. Z. J. Tamboli 9595907847

Mr. A. S. Sherikar 9527003375

Discipline

Oral PresentationMs. V.V. Dashwant

8956162334

Poster Presentation

Mr. V. S. Mule9823916005

Committee Members

Oral PresentationOral Presentation

Abstracts Abstracts Abstracts

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DEVELOPMENT OF CONTROLLED POROSITY OSMOTIC PUMP TABLET FOR ORAL

ADMINISTRATION OF METFORMIN AND GLIPIZIDE

S. C. Patil, D. A. Bhagwat, J. I. Disouza

Research Student, Shivaji University, Maharashtra, India.

Dept. of Pharmaceutics, Tatyasaheb Kore College of Pharmacy, Warananagar,

Tal: Panhala, Dist: Kolhapur, MS. India

Abstract:

Osmotically controlled oral drug delivery systems (OCODDS) utilize osmotic pressure as the

energy source for the controlled delivery of drugs. Drug release from these systems is independent of pH

and hydrodynamic conditions of the gastro-intestinal tract (GIT) to a large extent, and release

characteristics can be easily adjusted by optimizing the parameters of the delivery system. A system that

can deliver multi- drug at prolonged rate is very important for the treatment of various chronic diseases

such as diabetes, asthama and heart diseases. Type 2 diabetes mellitus emerges as a result of multiple

pathophysiologic changes. If the pharmacotherapy of type 2 diabetes should be tailored to the underlying

pathophysiology, it would be necessary to use a combination of agents with complementary mechanisms

of action. The two principal defects in type 2 diabetes are insulin deficiency and insulin resistance.

Therefore, combining an insulin-providing agent with an insulin-sensitizing agent will augment the

efficacy of current antihyperglycemic agents. The objective of present study is to develop controlled

porosity osmotic pump (CPOP) that can deliver drugs having complementary mechanism of action i.e.

insulin providing agent (glipizide) with insulin sensitizing agent (metformin) in controlled manner upto

12 hrs. It was prepared by incorporating drugs in the core and coated with various types (PVP, PEG 400,

HPMC) and levels (30, 40, 50 % w/w of polymer) of pore former at weight gain of 8, 12 and 15%.

Keywords: Osmotic controlled oral drug delivery system, solid dispersion, spray drying etc.

AICTE Sponsored

Evolving Pharmaceutical Regulatory & Quality System FrameworkNational Level Conference on


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RAPID EPITOPE ANALYZER FOR DIAGNOSIS AND PROGNOSIS OF COLON CANCER

CELLSPatil A.R,, Patil K.S, Desouza J.I.

Tatyasaheb Kore College of Pharmacy, Warananagar,Tal-Panhala, Dist- Kolhapur-416113, India.

Email: -

Abstract:

Helix pomatia agglutinin (HPA) is a lectin that has been used extensively in histopathology, since

its binding to tissue sections from colon cancers is correlated with the worst prognosis for the patients.

The lectin recognizes á-d-N-acetylgalactosamine (áGalNAc) containing epitopes which are only present

in cancer cell lines having a high likelihood to undergo metastasis, such as the HT29 cancer colon cell

line. Several colon cancer cell lines have also been shown to be labeled, although IGROV1, an ovarian

cancer cell line, is not. The crystal structures of the lectin complexed with two GalNAc containing

epitopes associated with cancer, the Tn (áGalNAc-Ser) and Forssman (áGalNAc1-3GalNAc) antigens,

show the lectin's specificity for GalNAc is due to a particular network of hydrogen bonds. These

structures provide the molecular basis for the use of HPA in metastasis research. Use of bio informatics

tool like ELF (Epitope location finder) is software based technique used to find out exact epitope from

group of the antigenic protein but fail in case of cancer detection. So new bio-informatics tool devised

proved to be promising method that will help in the diagnosis and prognosis of the cancer therapy via

software given name “Kiabhi” that constructed specially for colon cancer available at

.Revalidation is carried by HPA that bind only with colon cancer cell as antigen

antibody reaction.Keywords: HPA (helix pomata agglutinin), á-d-N-acetylgalactosamine (áGalNAc).

[email protected]

www.abhikiran.com

AICTE Sponsored



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RAPID EPITOPE ANALYZER FOR DIAGNOSIS AND PROGNOSIS OF COLON CANCER

CELLSPatil A.R,, Patil K.S, Desouza J.I.

Tatyasaheb Kore College of Pharmacy, Warananagar,Tal-Panhala, Dist- Kolhapur-416113, India.

Email: -

Abstract:

Helix pomatia agglutinin (HPA) is a lectin that has been used extensively in histopathology,

since its binding to tissue sections from colon cancers is correlated with the worst prognosis for the

patients. The lectin recognizes á-d-N-acetylgalactosamine (áGalNAc) containing epitopes which are

only present in cancer cell lines having a high likelihood to undergo metastasis, such as the HT29 cancer

colon cell line. Several colon cancer cell lines have also been shown to be labeled, although IGROV1, an

ovarian cancer cell line, is not. The crystal structures of the lectin complexed with two GalNAc

containing epitopes associated with cancer, the Tn (áGalNAc-Ser) and Forssman (áGalNAc1-

3GalNAc) antigens, show the lectin's specificity for GalNAc is due to a particular network of hydrogen

bonds. These structures provide the molecular basis for the use of HPA in metastasis research. Use of bio

informatics tool like ELF (Epitope location finder) is software based technique used to find out exact

epitope from group of the antigenic protein but fail in case of cancer detection. So new bio-informatics

tool devised proved to be promising method that will help in the diagnosis and prognosis of the cancer

therapy via software given name “Kiabhi” that constructed specially for colon cancer available at

.Revalidation is carried by HPA that bind only with colon cancer cell as antigen

antibody reaction.Keywords: HPA (helix pomata agglutinin), á-d-N-acetylgalactosamine (áGalNAc).

[email protected]

www.abhikiran.com

AICTE Sponsored



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KINETIC STUDIES OF MUTUAL PRODRUG OF DICLOFENAC AND

METHOCARBAMOL

Bhatia N. M., Chucha K., *Shirsat V.B.,

Bharati Vidyapeeth College of Pharmacy, Department of Quality Assurance,

Near Chitranagari, Kolhapur 416013, India.

E-mail: [email protected]

Abstract:

Prodrug has been the concept of retro metabolic drug design that incorporates targeting,

metabolism and the duration of action consideration into the design process. Prodrug of Diclofenac was

prepared by condensation with methocarbamol. The synthesized prodrug was characterized and 1confirmed by physicochemical, IR, H NMR and MS. In vitro hydrolysis was studied in pH ranging from

1.2 to 7.4, simulated gastric fluid (SGF), simulated intestinal fluid (SIF) where it was found to be stable,

and in human plasma and liver homogenate where it was rapidly hydrolyzed, suggesting that the prodrug

remains unhydrolysed in the stomach however rapidly cleaved by esterase in blood to give the parent

drug after absorption. Result of protein binding study shows that the synthesized prodrug binds to a less

extent as compared to the parent drug. The ester prodrug was evaluated for analgesic, anti inflammatory

activity, skeletal muscle relaxant activity and was found to possess comparable activity with that of the

parent drugs. Furthermore, T.S. specimen of the stomach also shows significant reduction in gastric ulcer

formation to mice gastric mucosa as compared to parent carboxylic acid drug.

Keywords: Diclofenac, Methocarbamol, non-steroidal anti-inflammatory drugs (NSAIDs), skeletal

muscle relaxants, analgesic activity, anti inflammatory activity.

AICTE Sponsored



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IMPACT OF IMPURITIES ON QUALITY, SAFETY OF DRUGS AND

PHARMACEUTICALSSrinivas Reddy B*, Ramya Rathod B, Venkata Sai T, Dr. Prathima Srinivas

Sri Venkateshwara College of Pharmacy and Research Centre, Madhapur, Hyderabad -500081

Abstract:

Pharmaceutical impurities are the unwanted chemicals that remain with the active

pharmaceutical ingredients or developed during formulation or upon aging of both API and formulation.

Tremendous attention is given for the impurity profiling of pharmaceutical products in present time.

Impurities can endanger the human health by affecting quality, safety and efficacy of the products. Due to

the noticeable impacts of impurity on quality of pharmaceuticals, impurity control in pharmaceutical

products is a primary goal of drug development. To assure the quality and safety of drugs, impurities must

be monitored carefully. It is important to understand what constitutes an impurity and to identify the

potential sources of such impurities, the control of pharmaceutical impurities in a pharmaceutical

industry is an important task to the formulator. ICH has formulated workable guidelines regarding the

control of impurities in pharmaceutical drug substance as well as formulations. It is necessary to isolate

and characterize an impurity and therefore selective analytical methods are developed to monitor them.

We present here a description of different types of impurities and their origin in relation to ICH

guidelines, specification limits and qualification of impurities. Different methods of impurity profiling

which are routinely used for monitoring impurities, the causes of degradation and the possible remedies

shall also be discussed with case studies.

Keywords: Quality, Safety, Impurity profiling, Qualification threshold

AICTE Sponsored



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USE OF ERYTHRINA INDICA FLOWER EXTRACT AS A NATURAL INDICATOR IN

ACID BASE TITRATION

Rahul Londhe*,Ramling Patrakar.

Shree Santkrupa College of Pharmacy, Ghogaon (karad) Maharashtra, India 415111

Email:[email protected]

Abstract:

Erythrina indica, also known as Indian coral tree, it is a medium-sized, spiny, deciduous tree. It is native

to the tropical and subtropical regions of eastern Africa, the Indian Subcontinent, northern Australia, and

the islands of the Indian Ocean The present work highlights the use of Erythrina Indica Flower extract as

an acid base indicator in different types of acid base titrations. The equivalence points obtained by the

flower extract coincided with the equivalence points obtained by standard indicators. In case of weak

acid and weak base titration, the results obtained by the flower extract matched with the results obtained

by mixed indicator. This natural indicator was found to be a very useful, economical, simple and accurate

for the said titration.

Keywords: Erythrina Indica Flower, Acid base indicator, Natural indicator.

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IN SILICO DESIGN OF NOVEL GP120 BINDING LIGANDS; BASED ON COMBINING

APPROACH OF STRUCTURE BASED AND LIGAND BASED DRUG DESIGN

Vinayak D. More*, Dr. M. S. Bhatia, R. B. Nikam.

Department of Pharmaceutical chemistry, Bharati Vidyapeeth's College of Pharmacy, near

chitranagari, Kolhapur. Maharashtra. India.416013.

Abstract:

Sequential CD4 receptor and co-receptor binding are prerequisites to start fusion of viral (HIV)

envelope and cellular membrane. The viral envelope glycoprotein mainly gp120 interacts with CD4

receptor. However, no approach to targeting the CD4-gp120 interaction has been successful in clinical

practice. The high-resolution three-dimensional structures of gp120 were downloaded from

bioinformatics online tool. VLife Molecular Design Suite (MDS) version 3.5 has been applied to get

cavities in the gp120 structure and online sever tools Pocket Finder and Q-site Finder tools were applied

for modeling novel binding sites in gp120. The various regions of these sites were studied for

complementary electrostatic potential, complementary hydrophobicity, probable hydrogen bonding, pi-

stacking and other interactions. Furtherly chemical database was extensively used to design novel

ligands. Refinement of this design was done by KNN based 3D QSAR study of already reported gp120-

CD4 inhibitors. The k-nearest neighbor (kNN) technique supported with Stepwise (SW) forward

selection method was exclusively applied. Data set of 32 molecules was used to develop four different 3D

QSAR models. The highly significant two models describe different descriptors along with their range of

field values for hypothetical pharmacophore with maximum binding affinity. These results were

exploited to make design of novel potential ligand more rational and more specific. Proposed chemical

scaffolds are versatile and key for further development of novel class of anti-HIV drugs.

[email protected]

AICTE Sponsored



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NEW APPROACH TO HPLC METHOD DEVELOPMENT FOR

SIMULTANEOUS ESTIMATION OF ATORVASTATIN CALCIUM AND

FENOFIBRATE IN RABBIT PLASMAa b a a

Somnath D. Bhinge , S.M. Malipatil , Abhijeet Jondhale , Anil S Savali , Chandrakant S c

MagdumA B C

RMES's College of Pharmacy, Gulbarga, HKES's College of Pharmacy, Gulbarga. Rajarambapu College of

Pharmacy, Kasegaon.

Abstract:

An accurate, rapid and simple reversed-phase high performance liquid chromatography (RP-HPLC)

method was developed and validated for simultaneous estimation of atorvastatin calcium and fenofibrate

in rabbit plasma. Plasma samples were treated with acetonitrile to precipitate proteins. Chromatographic

separation was accomplished using CAPCELL PAK C (4.6mm x 250mm, 5 m) analytical column with a 8

mobile phase consisting of phosphate buffer and acetonitrile (28:72). Detection and quantification were

performed by UV/Vis detection at 260 nm. The lower limits of detection and quantification were 0.05 µg -1 -1 -1 -1

mL and 0.20 µg mL for atorvastatin calcium and 0.07 µg mL and 0.35 µg mL for fenofibrate -1

respectively. The calibration curves are linear over the concentration range 1 to 40 µg mL for both

atorvastatin and fenofibrate in rabbit plasma. The method was quantitatively evaluated in terms of

linearity, precision, accuracy, recovery, selectivity, and stability. The proposed method is simple,

convenient and suitable for the analysis of atorvastatin calcium and fenofibrate in rabbit plasma.Keywords - Atorvastatin calcium, fenofibrate, rabbit plasma, High performance liquid chromatography.

AICTE Sponsored



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NOVEL CONCEPT OF FORMULATION AND EVALUATION OF PARACETAMOL DISPERSIBLE TABLETS

*1 1Chinmay Anand , Prof. Dr. G. Vidya Sagar

1Veerayatan Institute of Pharmacy, Jakhaniya, Tal. Mandvi-Kutch 370460, Gujarat, India

*Corresponding Author: [email protected]

Abstract: The dosage forms with rapid dispersion technology, fast dissolving technology have received ever-increasing demand during last decade, due to its better patient compliance. The acceptance from a unique class of customers and utility of formulation in several aspects resulted in development of various novel concepts of fast dissolving tablets and dispersible tablets. As per world Health Organization, Tablets are solid dosage forms usually obtained by single or multiple compressions of powder or granules. Development of Oral Dispersible Tablets of Acetaminophen or Paracetamol is selected due to its popularity and availability in a variety of therapeutic markets, both Over the Counter OTC and by Prescription. Formulation aspects behind selection of API are its bitterness, insoluble and poor compressibility properties. Paracetamol is mild anti-inflammatory drug with potent antipyretic and analgesic action. The proposed formulation of Novel dispersible tablets with Paracetamol is combined of a base mixture of drug and polyacrylic copolymer A Cationic Ion Exchange resins in various proportions among other pharmaceutically acceptable components. Various combination of formulation with drug resin and artificial sweetener such as Aspartame, Neotame, Suchralose, and Saccharin with super Disintegrant such as Crospovidone, Croscarmellose Sodium, and Primogel used in formulation trial F- 1 to F - 8. Direct compressible grade of diluents with direct compression method are used for compression of tablets. The prepared formulations were evaluated for various physical parameters such as uniformity of weight, thickness variation, hardness, friability test, dispersion test, wet ability test, disintegration test for tablets, physical evaluation of blend such as particle size distribution, bulk density of granules, angle of repose, water and moisture content . The prepared formulations were also evaluated for various analytical parameters such as content uniformity, assay, and drug release of tablets with dissolution test. Physical evaluation for bitterness was performed by using human volunteer to confirm the acceptance of taste. Among the variation formulations formulation F-5 was found satisfactory on the basis of physicochemical evaluation and taste evaluation.KEYWORDS: Dispersible Tablets, Paracetamol, Dissolution Test, Super Disintegrant, Taste Masking Resins

AICTE Sponsored



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IN-VITRO CYTOTOXIC PROPERTY OF METHANOL EXTRACT OF SEEDS OF

CUCURBITA PEPOKulkarni A. A*, Adnaik R. S, Magdum C. S, Mohite S. K

Rajarambapu College Of Pharmacy, Kasegaon Dist Sangli (MS)*Email:

Abstract:

Cucurbita pepo (C.pepo) is a perennial creeping herb belonging to family Cucurbitaceae. It

mainly contains fixed oils such as Linoleic acid, stearic acid, oleic acid, palmitic acid and others. Along

with these it also shows the presence of cucurbitacins, vitamins and minerals. The plant is used in the

treatment of many diseases like anti inflammatory, diuretic, antimutagenic, antiulcer. The present study

was undertaken to screen cytotoxic activity of C.pepo. Different extract of C.pepo were taken and brine

shrimp lethality bioassay was done to analyse the activity. The lethality of methanol extract of C.pepo

with the brine shrimp was evaluated. The cytotoxicity shown by this fraction indicates the presence of

potent bioactive compounds and antitumor activity which can have clinical and therapeutic application in

most life threatening diseases like tumours or cancer.

[email protected]

AICTE Sponsored



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MODIFYING POLYMERS TO NEW EDGE: GRAFTING*Shaikh K. N., Payghan S. A., Disouza J.I.

*Dept. of Pharmaceutics, Tatyasaheb Kore College of Pharmacy, Shivaji University,

Warananagar, Kolhapur, Maharashtra, India (416113).

Abstract:

Polymers play an essential role in the emergence of the modern world. Though polymers are

legion, sometimes they cannot fulfil the demand, depending on their properties. Improvements in

polymers are tremendously important because they will widen the scope of application. One trend in

modern civilization is to effect gradual replacement of natural materials with either all synthetic materials

or modified natural materials. In the polymeric age, it is essential to modify the properties of a polymer.

Modification is essential to meet various challenges. The next generation awaits polymer modification as

it opens up new possibilities. Surface and bulk properties can be improved easily by modifying

conventional polymers. Sometimes, balancing of properties is needed, and this is possible only through

modification of polymers. Polymer modification is required to bring specific properties to the modified

material, such as enhanced thermal stability, multiphase physical responses, compatibility, impact

response, flexibility, and rigidity. Thus polymer modification improves the processibility of the

polymers. There are several means to modify polymer properties like blending, grafting, and curing, but

grafting is one of the promising methods. Grafting can be done by chemical treatment, photo-irradiation,

high-energy radiation technique, etc. Several factors control grafting. In the past years grafting has been

emphasised for its various applications. The modified polymers through grafting have a bright future and

their development is practically boundless.

[email protected]

AICTE Sponsored



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Abstracts Abstracts Abstracts

Poster PresentationPoster Presentation

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COMPARATIVE STUDY OF GLIMEPIRIDE TABLETS OF VARIOUS MARKETED

FORMULATION*Devkar.Swati R ,Devkar preeti R, I.D.Raut ,S.K.Mohite

Rajarambapu College of Pharmacy, Kasegaon, Tal.Walwa, Dist Sangli-415404, M.S. India, E-

mail: [email protected]

Abstract:

The objective of present study was to evaluate different parameter like Weight Variation, Hardness

testing, Friability testing, Disintegration testing and Dissolution testing of Glimepiride tablets of

various marketed formulation.

is an anti-diabetic drug (sulfonylurea-type) used along with a proper diet and exercise

program to control high blood sugar. It is used in patients with type 2 (non-insulin-dependent

diabetes). It is available as different brand in market like Glimy ,Glimestar, Glador, Glimiprex etc.

Glimepiride was completely absorbed, with peak plasma concentration (Cmax) of 88±21ng/ml attained

at 2.7±1.4 hours. The areaunder the plasma concentration-time curve (AUC) increased linearly .Protein

binding of glimepiride is >99.5% and the volum of distribution is 8.8 L.Glimepiride is mainly

metabolized in the liver. Its major metabolites are hydroxymethyl and carboxyl derivatives. The total

body clearance (CL/F) of glimepiride is 2.7 to 3.2 L/h after a single oral dose. Comparative study of

Glimepiride of various marketed formulation was carried out by estimating different parameters

like,weight variation,Hardness test, Thickness, Disintegration test and In vitro drug release study.From

the results of these parameter it was concluded that Glimiprex is a better release formulation than the

other formulation of glimepiride as it shows better drug release,and less percent friability.

Keywords: Glimepiride tablets of various marketed formulation,Weight Variation, Hardness testing,

Friability testing, Disintegration testing , Dissolution testing

Glimepiride

diabetes

AICTE Sponsored



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FORMULATION AND EVALUVATION EXTRUSION - SPHERONIZER & MODIFIED

DRUG DELIVERY SYSTEM PELLET

Mr.Krishnakar G. Patil

RRKS's college of Pharmacy, Bidar, Karnatka-India

Email: [email protected]

Abstract:

Aim: To Formulation Development & Evaluation of modified drug delivery system pellet Terbutaline

Sulphate, for the treatment of nocturnal asthma. Objective: To formulation & Evaluation of

Terbutaline Sulphate pellet 5mg dose used in treatment for nocturnal asthma. Pellets provide a

reduction in the dosage regimen & gastrointestinal irritation, minimizing the dose dumping effect

moreover controlling the drug release & increasing the absorption of the active ingredient. The

reproducibility of the release characteristics from pellet formulation is also much better with respect to

the single-unit dosage forms. “The drug release characteristic of time. Course and/or location are

chosen to accomplish therapeutic or convenience objective not offered by conventional dosage forms”

Methods: extrusion spironization, Wurster Coating method is used to preparation of modified drug

delivery system pellet.

Composion: Extrusion - spheronizer: To prepared extrusion spironization to required unit composion

Mannitol powder, Lactose Anhydrous, Klucel LF, Avicel 101, prednisone, Sodium Lauryl sulphate,

Disodium hydrogen phosphate, Distill water. Coating solution Preparation for optimization

formulation: To prepared unit composion Seal coating (20%) such as Pearlitol SD 200, Purified

Water. To prepared unit composion enteric Coating (15%) such as Hydroxyl propyl methyl cellulose

phthalate (HP 55), TEC, Isopropyl Alcohol, Dichloro methane. Result: Dissolution: 0.1 N Hcl in

which 2hrs drug not released folled by 6.8 phosphate buffer 92 % drug release within 20 min by USP-I

by using 50 rpm. Future Scope: To prepared suitable dose for maximum quantity of drug and also

increase safty, efficacy and maximum absorption of dose and reduce the dosage dumping by increasing

the bioaivbility of drug and formulation of suitable pharmaceutical formulation.

AICTE Sponsored



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ENHANCEMENT OF THE DISSOLUTION RATE OF A POORLY WATER SOLUBLE

DRUG BY FORMATION OF SPRAY-DRYING OF

SOLID LIPID NANOPARTICLESSunita S.Shinde, Avinash H.Hosmani

Research Scholar, JJT University, Jhunjhunu, Rajasthan, IndiaDept. of Pharmaceutics, Tatyasaheb Kore College of Pharmacy, Warananagar,

Tal: Panhala, Dist: Kolhapur, MS. India

Abstract:

The slow dissolution rate exhibited by poorly water-soluble drugs is a major challenge in the drug

development process. Following oral administration, drugs with slow dissolution rates generally show

erratic and incomplete absorption which may lead to therapeutic failure. The aim of this study was to

improve the dissolution rate and subsequently the oral absorption and bioavailability of a model poorly

water soluble drug,by formation of Spray-drying of solid lipid nanoparticles.SLN are interesting

colloidal drug delivery systems, since they have all the advantages of fat emulsions and polymeric

nanoparticles They open a broad field of applications including i.v.,oral and dermal administration. The

less cost-intensive spray-drying technique was investigated for SLN as an alternative method to

lyophilization. Spray-drying is widely used in the chemical, the food and the pharmaceutical industries.

It is commonly used to process milk, eggs, ceramics and fertilizers. It converts a liquid into a dry system

in a one-step process and can produce fine, dust-free powders as well as agglomerated ones, to precise

specifications. In our laboratories, we have used spray drying in an attempt to enhance the dissolution

rate of a model drug, In this paper, we report on the preparation and characterization of these particles and

their in vitro dissolution.

Keywords: Solid lipid nanoparticles (SLN); Spray-drying; surfactants, in-vitro study.

AICTE Sponsored



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ANTIMICROBIAL STUDY OF HIBISCUS SABDARIFFA LINN LEAVES EXTRACTSudhir S. Mulay*, Kailash R. Biyani

Anuradha College of Pharmacy, Chikhali, Dist. Buldhana (M.S.)[email protected]

Abstract:

Higher plants have been exploited as a source of biologically active compounds since antiquity.

The microbial disease is one of the most frequently occurring diseases in all ages, races and sexes.

Current strategies to overcome the global problem of antimicrobial resistance include research in

finding new and innovative antimicrobials from plants. The purpose of this study was to evaluate the in-

vitro antibacterial activity of ethyl acetate and aqueous extracts of leaves of Hibiscus sabadariffa linn

using the agar plate and MIC method against human pathogenic micro-organisms. In both the methods,

ethyl acetate fraction exhibited good inhibitory activity. Ethyl acetate fraction showed good inhibitory

property with MIC values ranging from 62.5µg/ml to 125µg/ml. Aqueous extract showed moderate

activity with MIC values ranging from 125 to 250µg/ml while the 95% ethanol showed minimum

activity ranging from 250 to 500µg/ml.

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ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF XANTHIUM

STRUMARIUM L.* 1 2Mohammed Rageeb Mohammed Usman , Shaikh Sajid R. , Md. Abullais Md. Usman

*Ph. D Scholar, JJT University, Rajasthan - 333001, India.1Oriental College of Pharmacy,Sanpada, Navi-Mumbai - 400705, Maharashtra, India.

2Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda - 425107, Maharashtra, India. E-

mail: [email protected]

Abstract:

Xanthium Strumarium L. compositae, is a common weed found in India. The whole plant, specially root

and fruit, is used as medicine. According to ayurveda, Xanthium Strumarium L. is anthelmentic,

antipyretic, antiepileptic, diuretic, cooling laxative, fattening, alexiteric, and tonic, digestive and

improves appetite, voice, complexion, and memory. The petroleum ether extract of Xanthium

Strumarium L. was evaluated for analgesic and anti-inflammatory activity at the doses of 250 and 500

mg/kg body weight. The tail immersion and acetic acid writhing response in mice were used to assess

analgesic activity. The acute toxicity study of the extract had shown no sign of toxicity up to a dose level

of 2000mg/kg body weight. Carrageenan induced paw edema in rats, which is an acute model used to

assess anti-inflammatory activity. The extract has inhibited paw edema in dose related manner. A dose

dependent analgesic action was obtained against tail immersion and writhing test indicating that

analgesic activity may be involved in the inhibition of the pain. Thus the petroleum ether extract of

Xanthium Strumarium L. possess significant analgesic and anti-inflammatory activities.

Keywords: Xanthium Strumarium L., Anti-inflammatory, Analgesic, Writhing, Phytomedicines.

AICTE Sponsored



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AICTE Sponsored



PHYTOCHEMICAL AND PHARMACOGNOSTIC EVALUATION OF ERYTHRINA INDICA LINN. LEAVES

1 2 3A. M. Patel *, S. M. Kurbetti , R.V. Savadi

1*Research Scholar, Shri Jagdishprasad Jhabarmal Tibrewala University, dist. Jhunjhunu, Rajasthan-

333001, India. E-mail :- [email protected]. of Pharmacognosy and Phytochemistry, SGM College Of Pharmacy Mahagaon,

Gadhinglaj. Kolhapur, Maharashtra, India.3Principal, Arvind Gavali College Of Pharmacy, Satara, Maharashtra, India.

Abstract:

Erythrina indica Linn (Febaceae) is a medium-sized, spiny, deciduous tree normally growing to 27 meter

tall, found in Bengal and many parts of India especially in southern and western India. The current study

was carried out to provide requisite pharmacognostic and phytochemical details about the plant. In the

microscopic studies, the leaves showed the presence of branched trichomes, vascular bundles, palisade

cells, collenchyma cells, spongy parenchyma and paracytic type of stomata. The total ash, acid insoluble

ash, water-soluble ash and sulfated ash were observed to 5.1±0.2%, 2.0±0.3%, 2.9±0.2% and 6.0%

respectively. Water soluble, alcohol soluble and petroleum ether soluble extractive values were found to

be 18±1.1%, 11±1.0% and 7±0.9% respectively. Moisture content was found to be 3.8±2.0%. The dried

leaf powder was subjected for soxhlet extraction using ethanol for 5 hours and for maceration by using

water for 48 hours. The aqueous extract of leaves revealed the presence of Glycosides, Alkaloids,

Carbohydrates, Tannins, Proteins, Saponins, Flavonoids, and Phenolic Compounds, while ethanolic

extract of leaves showed the presence of Alkaloids, Flavonoids, Saponins, Proteins, Steroids,

Carbohydrates, Tannins, and Phenolic compounds. The result of this study can be useful in setting

some diagnostic indices for the identification and the preparation of the monograph of the plant. Keywords : Erythrina indica, Febaceae , Phytochemical, Tannins, Traditional medicines.

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CHROMATOGRAPHIC QUANTIFICATION OF CAFFEINE, CHLORPHENIRAMINE

MALEATE AND DICLOFENAC SODIUM FROM THEIR NOVEL MULTICOMPONENT

FORMULATION AND PLASMA

R. A. Patil*, S. J. Melawane and M. S. Bhatia

Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra (India) 416 013

Abstract:

Reversed phase High performance liquid chromatography method (RP-HPLC) for separation

and estimation of Caffeine, Chlorpheniramine Maleate and Diclofenac Sodium from their

multicomponent tablet formulation and biological fluids was developed. The separation of three

drug components was carried on a JASCO HPLC system with HiQ-Sil C18HS column (250 x 4.6 mm,

5µm) using methanol: 0.1% sodium salt of n-hexane sulfonic acid (60:40 v/v) pH adjusted to 4 with 0.1

M orthophophoric acid as mobile phase by isocratic elution with a flow rate of 1.0 ml/min and detection

wavelength of 255 nm. Ezetimibe was used as internal standard. For formulation analysis method,

Beer's law was obeyed in the concentration range of 2 to 130 µg/ml of Caffeine, 1 to 96 µg/ml of

Chlorpheniramine Maleate and 2 to 200 µg/ml of Diclofenac Sodium. For plasma analysis method,

Beer's law was obeyed in the concentration range of 50 to 300 ng/ml of Caffeine, 50 to 300 ng/ml

of Chlorpheniramine Maleate and 100 to 600 ng/ml of Diclofenac Sodium. The developed method

successfully estimates drugs from their tablet formulation and plasma with mean assay values of 99.24 ±

0.86 and 89.59 ± 2.67 % for Caffeine, 98.92 ± 0.98 and 88.28 ± 1.92 % for Chlorpheniramine Maleate

and 99.83 ± 0.68 and 92.58 ± 1.93 % for Diclofenac Sodium respectively. The method for formulation

and plasma analysis were validated as per the ICH Q2B (R1) and USFDA guidelines respectively. The

results of validation studies proved applicability of method in biopharmaceutical and pharmacokinetic

studies of these drugs.

Key Words: RP-HPLC, Caffeine, Chlorpheniramine Maleate and Diclofenac sodium.

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USE OF ERYTHRINA INDICA FLOWER EXTRACT AS A NATURAL INDICATOR IN

ACID BASE TITRATION

Rahul Londhe*, Ramling Patrakar.

Shree Santkrupa College of Pharmacy, Ghogaon (karad) Maharashtra, India 415111

Email:[email protected]

Abstract:

Erythrina indica, also known as Indian coral tree, it is a medium-sized, spiny, deciduous tree. It is native

to the tropical and subtropical regions of eastern , the , northern , and

the of the The present work highlights the use of Erythrina Indica Flower extract as

an acid base indicator in different types of acid base titrations. The equivalence points obtained by the

flower extract coincided with the equivalence points obtained by standard indicators. In case of weak

acid and weak base titration, the results obtained by the flower extract matched with the results obtained

by mixed indicator. This natural indicator was found to be a very useful, economical, simple and

accurate for the said titration.

Key Words: Erythrina Indica Flower, Acid base indicator, Natural indicator.

Africa Indian Subcontinent Australia

islands Indian Ocean

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SELF-EMULSIFYING DRUG DELIVERY SYSTEM A NOVEL APPLICATION IN DRUG DELIVERY OF HYDROPHOBIC DRUGS

*1 2 3Prasad Patrekar , A. H. Hosmani , I. D. Gonjari

1 Adarsh Institute of Pharmacy, Vita. Tal-Khanapur, Maharashtra, India.415311

2 Govt. College of Pharmacy, Ratnagiri, Maharashtra, India. 415612

3 Assistant Director AICTE, New Delhi - 110001

Corres.Author:

Abstract: Oral route for drug administration is the easiest & most convenient. Poor aqueous solubility is the

major problem in oral formulation due to the rate of dissolution and rate of permeation through biomembrane are two critical slower rate determining processes in orally administered drug. About 40% new active entities are poorly water soluble or hydrophobic in nature. These poorly water soluble drugs create challenges to scientist to develop enhanced bio-avaibility for oral formulations. Various technological strategies are reported including derivatization, solid dispersions, cyclodextrin complex formulations, micronization and more to enhance bioavaibility. Apart from these self-emulsifying drug delivery system (SEDDS) is much focused due to its excellent efficiency in delivering poorly water soluble drugs with enhanced solubility, dissolution, absorption and bioavaibility. SEDDS are lipid based formulations. These systems are ideally isotropic mixture of oil, surfactants & co-solvents that form fine oil-in-water (o/w) emulsion or micro emulsion on mixing with aqueous phase with little or no energy input. With SEDDS bioavaibility of the drug increased & quantity of drug which is required for desired effect is decreased. This review article explains how self-emulsifying drug delivery system can enhance solubility & bioavaibility of drug & SEDDS technology is a novel application in drug delivery and solve problems associated with delivery of hydrophobic drugs.

[email protected]

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SIMULTANEOUS ESTIMATION OF CANDESARTAN CILEXETIL AND

HYDROCHLORTHIAZIDE IN TABLET DOSAGE FORM BY UV

SPECTROPHOTOMETRIC METHOD.

Bhadke Tejaswini K*, Kesur Bhavik R, Mohite Shrinivas K, Magdum Chandrakant S.

Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, India.


Abstract:

Candesartan cilexetil is a prodrug of candesartan which belongs to angiotensin II receptor blocker.

Hydrochlorthiazide is a diuretic. Both of the drugs are used in treatment of hypertension. A simple,

sensitive and reproducible UV Spectrophotometric method was developed using the solvent 0.1 N

NaOH for simultaneous estimation of candesartan cilexetil and hydrochlorthiazide in tablet dosage

form. Candesaratan cilexetil and hydrochlorthiazide exhibited absorbance at 251nm and 273nm in 0.1N

NaOH and followed Beer's law in the concentration range of 4 28µg/ ml and 2 - 14µg/ml respectively.

The results of analysis were validated statistically and by recovery studies confirmed the accuracy and

precision of the method.

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SCHEDULE HX: COMBATING ANTIBIOTIC RESISTANCE ENHANCING PROFESSIONAL INTEGRITY OF INDIAN PHARMACIST

Patil A.R., Biradar K. S., Patil K.S.Tatyasaheb Kore College of Pharmacy, Warananagar,

Tal-Panhala, Dist- Kolhapur-416113, India. Email: -

Abstract:Antibiotic resistance (AR) is burning question from some decades & result into 150000 deaths. The present study focused on causes of AR and to explore factors which influence malpractices of usage antibiotics and suggest corrective measure. Literature study from various magazines, websites, was done to understand whether misuse of antibiotic is key factor which increases the antibiotic resistance. 40 prescriber, 40 pharmacist, 40 patients & FDA officers were approached in urban & rural area of Kolhapur, Sangali district. Self-administered questionnaires were developed for study. Out of 120 of these participants 50% are from rural area and 50% from urban are taken for effective result in our survey. Total contributers in AR has been found as 55% prescribers, 27% pharmacist, 10% patients, 5% society. Various determinants are found which affect on antibiotic usage. Health ministry is planning to implement Schedule HX to combat AR. There are certain limitations which have not taken into consideration like, 1.Less availability of tertiary care hospitals, 2. Economic loss of Pharmacist, 3.Availability of medicines in emergency cases. 4. Tertiary care hospitals not affordable. This bridge can be connected by setup of governing body which is initiated in kolhapur by the name “thyrocare” and there is need of stringent regulation to combat this situation & strapping “iron will” in health care professionals to organise campaign to provoke awareness which we initiated. Keywords: Antibiotic resistance, Schedu le HX, Tertiary care hospitals.

[email protected]

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MULTIPATHWAY INDUCTION PROGRAM: PANACEA FOR IMPROVING PROFESSIONALISM IN NOVICE STUDENTS

Biradar K. S., A.R. Patil, Patil K. S., Tatyasaheb Kore College of Pharmacy, warananagar,

Tal- Panhala, Dist- Kolhapur- 416113. E-mail:

Abstract:Pharmacy education needs re-engineering for improving professionalism in students by innovative technique. The objective of this work it to design & define an induction program comprising set of programs for newly admitted students, to implement this induction program and to find the outcome of induction program on student behavior by continual assessment. A multi-pathway Induction Program is designed by arranging a FGD (Focused group discussion) in between 5 academicians & 2 industrialists. As per the conclusion induction program is arranged for two consecutive batches as they get admitted in college. Behavior assessment of these 2 batches and a first batch for which induction program was not conducted (N=198). Continual behavior assessment was carried out in 3 month, 12 month & 18, 24 month for second year batch & in 3 month for first year batch. Evaluation is carried out by taking responses from teachers (20) and mentor's (20), with help of questionnaire and their academic growth and behavior. The responses were graded with low (0-30%), moderate (31-70%) & high (71-100%). As per conclusion of FGD following programs such as 1.Welcoming to fresher's, 2. Introduction to institute, 3.Academic planer, 4.peer mentoring programs, 6.White coat ceremony, 7. Introduction to committees, 8. Outside speakers for update recent trends are arranged. Behavior assessment result found that newly admitted students have more professionalism, integrity, collaboration with faculty by arranging induction program. Hence institutions, administrators, practitioners, have to consider it for implementation in novice student for upgrading pharmacy profession.

Keywords: - behavior assessment, professionalism, Student integrity.

[email protected]

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FORMULATION AND DEVELOPMENT OF TASTE MASKED AMBROXOL

HYDROCHLORIDE SUSPENSION USING ION EXCHANGE RESINS

1 2Bharat V. Jain* , Dr. Shashikant D. Barhate

1 Ph.D Scholar, JJT University, Jhunjhunu, Rajasthan

2 Shri Sureshdada Jain Inst. of Pharmaceutical Education and Research, Jamner, M.S

Email id:

Abstract:

In the present work the attempt was made to prepare taste masked suspension of Ambroxol

Hydrochloride by abating the intensely bitter taste of Ambroxol Hydrochloride. Taste abatement was

done by complexing of Ambroxol hydrochloride with different Ion Exchange Resins (IER) like

Tulsion 335 and Indion 214 in different ratios. The prepared suspensions were evaluated for taste, drug

content, particle size, viscosity, sedimentation volume and drug release. The resonates prepared with

drug- T343 ratio (1:2) at pH 8, gave maximum drug loading. Suspension containing above resinates

showed more than 80% In vitro drug release within 30 min. Prepared formulation also showed good

stability and can retain its palatable taste. The developed formulation was an additional advantage like

simplification of manufacturing procedure and is economical. Thus, the “patientfriendly dosage

form” of bitter drugs, especially for pediatric, geriatric, bedridden, and noncooperative patients, can be

successfully formulated using this technology.

[email protected]

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HEPATOPROTECTIVE ACTIVITY OF EUPHORBIA HIRTA LINN. AGAINST CCL4 INDUCED LIVER TOXICITY IN RAT

Vinod V. Takale*1, R.V.Savadi 2, Ansar M. Patel 3*1 Research Scholar, Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Dist-

Jhunjhunu, Rajasthan, India. 333001 2 Principal, Arvind Gavali College of Pharmacy Satara, Dist- Satara, Maharashtra, India.

3 Principal Sant Gajanan Maharaj College of Pharmacy, Gadhingaj, Dist- Kolhapur, Maharashtra, India.

Abstract:

The leaves of Euphorbia hirta Linn. is said to be valuable in traditional medicine for the various diseases

including Hepatotoxicity. To give scientific background to the above traditional claim, the leaves of

Euphorbia hirta Linn. were evaluated for its Hepatoprotective activity. In the present study, shade dried

and coarsely powdered leaves were first defatted with petroleum ether (40-60) after that successively

extracted with methanol. The methanolic extract was concentrated & dried under reduced pressure, then

it is used for Pharmacological investigations. The toxicity studies were carried out as per OECD

guidelines and the doses were fixed at 200 mg/kg b.w. and 400 mg/kg b.w. The Methanolic extract was

evaluated for in vivo Hepatoprotective activity by using CCl4 induced hepatotoxicity model. The

Methanolic extract showed better effect at the dose of 400 mg/kg b.w in lowering elevated SGOT, SGPT,

ALP, total bilirubin and direct bilirubin levels in hepatotoxic rats. Histopathological studies showed that

the Methanolic extract showed minimal hepatocytes necrosis and Good number of binucleate

regenerating hepatocytes. Key words: Euphorbia hirta Linn, Hepatoprotective, CCl4, Methanolic extract.

E-mail- [email protected]

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PROTECTIVE EFFECT OF ANTI-OXIDANTS ON L-ARGININE INDUCED ACUTE

PANCREATITIS IN RATSSandeep Biradar*, Veeresh Bantal

G Pullareddy College of pharmacy, Mehdipatnam, Hyderabad-500028


Abstract:

Background: Development of acute pancreatitis, an inflammatory disorder of the exocrine part of

pancreas, involves oxidative stress and inflammatory mediators. The present study evaluates the

beneficial effect of myrcene and limonene, potential antioxidant and anti-inflammatory agent on L-

Arginine induced pancreatitis in rats. Methods:Wistar rats of either sex (n = 84) were divided into 7

groups (n = 12), Group 1: control (saline), Group 2: disease control (L-Arginine, 2.5 g/kg, i.p., two times

with one hour interval), Group 3, 4, 5 and 6: test groups (received myrcene 100 and 200 mg/kg, limonene

100 and 200 mg/kg, p.o., respectively one hour after the last injection of L-Arginine), Group 7: standard

group (methyl prednisolone 30 mg/kg, p.o., one hour after the last injection of L-arginine). Half of the

animals were sacrificed at 24 hours (study I) and remaining half were sacrificed at 72 hours (study II)

after the last injection of L-Arginine. Blood was collected for estimation of serum amylase and lipase

while isolated pancreas, lungs, liver and kidney for the estimation of bio-chemical parameters and

histological examination. Results: Administration of L-Arginine significantly developed acute

pancreatitis characterized by elevated levels of serum amylase and lipase at 24 hours compared to

normal control group. Treatment with myrceneand limonene dose dependentlydecreased the serum

amylase and lipase levels, restored the antioxidant status of pancreas. Treatment with myrceneand

limonene further restored the L-Arginine altered histoarchitecture of pancreas, lungs, liver and kidney.

Conclusion: Treatment with myrcene and limonene significantly ameliorated the L-Arginine induced

pancreatitis.

Keywords:Acute pancreatitis; L-Arginine; myrcene, limonene; Oxidative stress; Inflammation

AICTE Sponsored



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CONTENT ORIENTED ADVERTISEMENT: HALLMARK TO CONFER DETAILING OF

COUNTER-INTUITIVE PHARMACEUTICAL PRODUCTS Ghate B.U, Mohite Y.A, Patil K.S., D'souza J. I.

Tatyasaheb Kore College of Pharmacy, Warananagar, Tal- Panhala, Dist- Kolhapur-416113, India. Email:

Abstract:

Advertisement (Ads) is wide form of promotion of Counter-intuitive pharmaceutical product by

organizing paid time for advertising a message. The present study was aimed to identify various means of

pharmaceutical Ads and their evaluations regarding their benefits and hazards; to suggest better and

effective advertisement; development of new strategies to bring out effectiveness and for better

understanding of Ads. Evaluation of Ads from different Medias like TV (50), Newspaper (130),

magazines (50) and radio (5) considering factor such as safety, efficacy, and contra-indication was done

by panel. Panel consists of academicians, reputed pharmacist, and well known physician, FDA officers,

industrialist and layman-patient. Focused group discussion was conducted on product detailing &

content oriented Ads to reach the final conclusion. Survey revealed that ads from different Medias,

specially published in news paper are 1. Less understood or more confusing to consumer because of less

content details, brand logo, product detailing; 2. Misguiding and with wrong statements; 3. Without

emphasis on safety, side effects, contraindication etc. Certain Ads provoke the consumer's desire by

various visual methods and animations. Study concludes that today's health-conscious society demands

to know more about drug therapy. “Learned intermediary doctrine” is becoming outdated model of the

doctor-patient relationship. Content oriented Ads can be the best mean to serve the purpose.

[email protected]

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FORMULATION AND EVALUATION OF ORAL SUSTAINED RELEASE TABLET OF

PROPRANOLOL HCL USING INDION234 AS

ION EXCHANGE RESIN MATERIALGhorpade S.M.*, Gandhi S.M., Magdum C.S., Mohite S.K.

Rajarambapu College of Pharmacy,Kasegaon ,Tal-Walwa ,Dist-Sangli (MH)Email:- [email protected]

Abstract:

The major drawback of sustained release or extended release tablets is dose dumping, resulting in

risk of toxicity. The use of IER has occupied an important place in the development of controlled or

sustained release systems because of their better drug-retaining properties and prevention of dose

dumping. Propranolol HCl is a beta blocker agent administered on twice a day dosage regimen in the

treatment of hypertension.In the present study, resinates of Propranolol HCl were formulated using

Indion -234 resins.Drug loading process was optimized with respect to drug:resin ratio, duration of

stirring ,pH of loading solution, and resinates were characterized using IR spectroscopy for

compatibility study.

Optimum drug loading was seen at pH of 3.5 in drug-resin ratio of 1:1 at 3 hours of stirring using

magnetic stirrer.The matrix tablets were prepared by using resinate along with other excipients such as

Avicel PH-102, HPMC etc. and prepared tablets were analyzed for its thickness, hardness, friability,

weight variation, drug content and in-vitro release studies.Tablet thus formulated provided sustained

release of drug over a period of 12 hours with first order kinetics. The release of drug from resinate

controls diffusion of drug molecules through polymeric material into aqueous medium The results

suggest that the Indion-234 is useful in developing sustained release matrix tablets of Propranolol HCl.

Resins thus promises considerable utility in the development of oral sustained release drug delivery

systems.

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A MULTIDISCIPLINARY APPROACH TO PREVENT PRESCRIPTION ERRORS

ENSURING THE PATIENT SAFETYGandhi S.M.*, Mohite S.K. , Gujar P.P. , Ghorpade S.M.,

Rajarambapu college of pharmacy,Kasegaon ,Tal-Walwa ,Dist-Sangli (MH)[email protected]

Abstract:

Medicines are part of most people's lives. Any Mistakes with medication increases the risk of

something going wrong. The rational use of drugs begins with a correct prescription; nevertheless, this

stage is where more than 50% of all treatments present errors. Our aim is to detect the most frequent

prescription errors, in order to justify the implementation of pharmaceutical care services at the hospitals

as well as the inclusion of pharmacists as part of the health care team. Sample of 220 prescriptions were

evaluated for their appropriateness according to basic pharmacotherapeutic variables. We visited 12

physicians to know their prescription writing habit, process of decision making and view regarding

prescription errors. Also interviewed 60 pharmacist near karad ,kasegaon ,islampur(MH,India)to know

problems occurs during prescription interpretation.

We find that the quality of most of the prescription was unsatisfactory both in terms of layout and the

content of the drug prescribed. Many prescriptions had illegible handwriting, Confusing drug names,

Polypharmacy practice. There are no pharmacists advising patients at community pharmacies,

expanding irrational use of drugs. This may preventable by having computational systems and by having

more pharmacists for monitoring prescriptions, advising physicians and taking care of patients.

Prescription orders should include essential parameters related to patient and drugs. The pharmacist

should check with the prescriber if any information is missing or questionable. All health care

professionals should have a common vision and goal to prevent medication errors. A multidisciplinary

approach to solving the problem of prescription errors is required which ensuring the patient safety

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ENHANCEMENT OF DISSOLUTION OF CINNARIZINE BY

SOLID DISPERSION METHOD

Hemant R. Kakade,*1 Anilkumar J. Shinde, Harinath N. More

Department of Quality Assurance, Bharati Vidyapeeth College of Pharmacy,

Near chitranagari, Kolhapur. E mail- [email protected]

Abstract:

The aim of present study was to enhance the solubility of poorly soluble drug Cinnarizine by the binary

solid dispersion techniques, Consisting drug and polymers. Cinnarizine is H1 antagonist widely used in

treatment of the vertigo, motion sickness and vomiting. In the present work solid dispersion was prepared

with poloxamer188 and poloxamer407 by the use of solvent evaporation method with ratios 1:1, 1:2 to

1:5 of drugs and poloxamers respectively. Solid dispersions were characterized by IR spectroscopy, in-

vitro dissolution study and Scanning electron microscopy. The infrared spectra suggested that there was

no chemical interaction between Cinnarizine and poloxamers. Equilibrium solubility studies showed

that drug solubility was enhanced as the polymer content increased. Dissolution showed that more than

80% drug releases within 10 min, SEM analysis revealed that drug morphology was changed the particle

appear porous and size of particle also reduced.

Keywords: solid dispersion, Binary system, poloxamer188 & poloxamer407.

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MODIFIED RELEASE FORMULATION DRUG DELIVERY SYSTEM FOR

CARDIOVASCULAR DRUGS2, 3 2, 3 1 2

Patil Atul , Kundu Subrata , Srinivasan Ganga , Borkar Nitin1 2VES College of Pharmacy, Mumbai University ; VerGo Pharma Research Laboratories Pvt.Ltd ; JJT

3 University [email protected]

Abstract:VP108 (code named) is the drug used for treatment of cardiovascular diseases and often limited its application due to its limited pH dependant solubility & bioavailability, in the present research work an attempt has been made to design a platform drug delivery technology# to use solublizers and pH modifiers in the core such that release of solublizers from the core is modulated to alter the release of active in association with rate modulating polymers. The release of solublizer and design of polymeric rate controlling system is configured such that sufficient amount of pH modulator or Solublizer is released specific to pH and significantly improves the solubility & there by release of the active in the said medium or pH. Most of the drugs are either basic in nature or acidic in nature & have pH dependent solubility, surprisingly we have found that by modulating the release of solublizer or pH modifier from the dosage form we can enhance the solubility and or release of active in unfavorable pH region where drug is insoluble and doesn't have sufficient solubility. Experimental Design: Formulation Development: Model drugs from cardiovascular category having pKa between 8- 10 were selected. BCS solubility of the same was carried out to understand their solubility behavior. Different solublizers and pH modulators were identified to understand the concentration required to achieve sufficient solubilisation. The important task was establishing required amount of Solublizer in the core and providing a rate controlling polymeric coating on the core to control the release of Solublizer to modulate release of drug from core. Analytical Methods: A HPLC method was developed to detect Solublizer and active in the dissolution method, the release of Solublizer or pH modulator was targeted or altered to achieve desired release for the actives.Result and Discussion: A Solublizer release based modulated drug delivery system can be developed with mixture of polymeric materials in bead or tablet dosage form In present research work a successful attempt has been made to develop a platform drug delivery technology. To develop a robust formulation which is scalable & commercially viable optimization of each process steps & composition was done to achieve a similarity factor F2 more than 60. Attempt was made to understand the principle mechanism of drug release from formulation and it appears to be by diffusion mechanism based on data of mathematical modeling. Conclusion: A Solublizer and ER polymer based drug delivery system can be designed to provide uniform drug release across the pH conditions for poorly soluble drugs of cardiovascular categories. This Provides cost effective generic or life cycle management opportunity for the drug & formulation thus provides tremendous business potential by minimizing health care management cost. Acknowledgments: VerGo Pharma Research for providing research facility. #Note: This technology is patented and final approval is awaited for the patent application Ref: 329/MUM/2012.

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CACTUS : WONDER MEDICINAL PLANT EXPLORED FROM SEMIARID REGION

Adake A.K, Patil R.R, G.V.PatilRajarambapu College of Pharmacy, Kasegaon, Tal. Walwa, Dist Sangli 415404, MS, India.

E-mail: Abstract:

Plants have played a significant role in maintaining human health and improving the quality of human

life for thousands of years and have served humans well as valuable components of medicines,

seasonings, beverages, cosmetics and dyes . Ethnopharmacological studies on such herbs/medicinally

important plants continue to interest investigators throughout the world. One such plant, Opuntia ficus-

indica invites attention of the researchers worldwide for its pharmacological activities ranging from

anti-inflammatory to anticancer activities. Opuntia ficus-indica is also known as prickly pear cactus.

Prickly pears are also known as "tuna", " " or nopales, from the word nôpalli for the pads, or

nostle, from the Nahuatl wordnôchtli for the fruit; or cactus.. The prickly pear cactus is unique

among cacti, and in fact among all plants, in that each part of the plant may be used for some healthful

purpose. The fruit of the cactusalso known as the pulp or tunacan be eaten much like other fruits. Cactus

Fruit Opuntia contain a range of in ample quantities, notably substituted .

Identified compounds of medical significance include , , ,

, and .

Keywords: Pricly pear fruits, seeds and pads, antioxidants on plasma LDL cholesterol concentration,

vitamins and minerals, antihyperglycemic effects, immune-stimulating, BPH

[email protected]

nopal Nahuatl

paddle

alkaloids phenethylamines

3-methoxytyramine candicine hordenine N-

ethyltyramine tyramine

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COSMETIC REGULATIONS: A COMPARATIVE OVERVIEW1 * 1 1 2Shalaka V. Patil , Rohini S. Patil, Rahul S. Adnaik, Durgacharan A. Bhagwat

1. Rajarambapu College of Pharmacy, Kasegaon, Tal: Walwa, Dist: Sangli,

MS. India. E-mail:

2. Tatyasaheb Kore College of Pharmacy, Warananagar, Tal: Panhala,

Dist: Kolhapur, MS. India

Abstract:

In a world new cosmetic regulations are being promulgated at an alarming rate. In view of the ever

growing market of the cosmetic countries has been setting up their own directive to regulate such

products. The current regulations of cosmetics are stringent. There are different regulatory bodies

worldwide having their own regulations to ensure safety of the cosmetic products. The major cosmetic

market constitutes of European Union (EU), United States of America (USA) .The regulations in these

territories are used as a model for the developing world. There may be different specific regulatory

systems; they have a common goal of ensuring that cosmetic products are safe and properly labeled. In

the industrialized countries these regulations have evolved to the point where they are rather extensive

and, largely because the United States and European Union are the two largest markets in the world for

cosmetic products. The cosmetics market in India is growing at 15-20% annually, twice as fast as that of

the United States and European market. Indian cosmetic industry is matured enough and responsible to

ensure the quality and safety of its products. The cosmetic regulations in India are complex and time

consuming for pre marketing approval. It is therefore important for a cosmetic manufacture to

understand the difference in regulatory system in India when compare to USA and EU. The aim of the

present review is to compare the cosmetic regulations in USA, EU and India.

[email protected]

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SOLID SELF MICRO EMULSIFYING DRUG DELIVERY SYSTEM OF

CARDIOVASCULAR DRUG FOR ENHANCED BIOAVAILABILITY1 2Durgacharan A. Bhagwat*, John I. D'Souza

1. Research Scholar, JJT University, Jhunjhunu, RajasthanE-mail:

2. Tatyasaheb Kore College of Pharmacy, Warananagar, Tal: Panhala, Dist: Kolhapur, MS. India. E-mail:

Abstract:

Improvement of bio-availability of drugs is one of the greatest challenges in drug formulations. Self

micro emulsifying drug delivery systems (SMEDDS) have shown great promise for enhancing

bioavailability of low solubility compounds. Conventional SMEDDS are normally prepared in a liquid

dosage form that can be administered in soft gelatin capsules, which have some disadvantages especially

in the manufacturing process. Incorporation of a liquid self-emulsifying formulation into a solid dosage

form may combine the advantages of SMEDDS with those of a solid dosage form and overcome the

disadvantages of liquid formulations. In view of this, the present investigation was aimed at developing

Solid-SMEDDS for poorly soluble cardiovascular drug for enhanced bioavailability. In this study

solubility of drug was determined in various oil, surfactant and co-surfactant. Pseudoternary phase

diagrams were used to evaluate microemulsification existence area. Three component SMEDDS

formulation were established and selected combinations were exposed to spray drying using water

soluble solid carrier. S-SMEDDS formulations were tested for reconstitution properties and solid state

characterization. The in-vitro dissolution studies of S-SMEDDS filled into hard gelatin capsule and pure

drug were carried out. Results showed that the mean droplet size of all reconstituted S-SMEDDS were

very low and all were found to be <100 nm. Drug releases from S- SMEDDS formulations were found to

be significantly higher as compared with that of pure drug. Thus study concluded with S-SMEDDS

provides useful solid dosage form to improve solubility and dissolution rate of poorly soluble

cardiovascular drug and concomitantly bioavailability.

Keywords: Self microemulsifying drug delivery system, bioavailability enhancement

[email protected]

[email protected]

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FORMULATION AND EVALUATION OF FLOATING TABLET OF CAPTOPRIL

PATEL P.V., PATEL R.P., SHAH DHARMESH

SHRI JAGDISHPRASAD JABARMAL TIBREWALA UNIVERSITY

(JHUNJHUNU, RAJASTHAN),

Email id: - [email protected]

Abstract:

The present study was undertaken to prolong the release of orally administer. floating tablets of Captopril

was prepared by using different grade of hydroxypropylmethylecellulose. Formulations were optimized

using different viscosity grades of hydroxypropylmethylecellulose. Lactose and citric acid were used in

different concentration as a channeling and chelating agent to obtain best optimized formulation and

designed to prolong the gastric residence time (GRT). Formulations were evaluated by floating lag time

and in vitro drug release method. Results revealed that the effect of channeling and chelating agent at

different concentration had significant effect on the release of the drug from hydrophilic matrix tablet.

Three different viscosity grades of hydroxypropylmethylecellulose namely K100M, K 15M and K 4M

were used as a floating polymer or intention of polymer. It was observed that different viscosities not only

influence the drug release from hydrophilic matrix but they also affect the floating properties of tablets.

Dissolution profiles were subjected for various kinetic treatments to analyze the release pattern of the

drug and we found that drug release by diffusion mechanism and followed square root kinetics or

Higuchi's kinetics. The in vitro release profiles of drug from all the plots shows high linearity (r2= 0.9813

to 0.9954). Optimized formulations were again subjected for thickness, friability, hardness, uniformity

of content, uniformity of weight, in vitro dissolution, Floating lag time, floating time and stability

studies. Results revealed that the floating formulation of the Captopril is the best formulation to obtain

better therapeutic effect and hydroxypropylmethylecellulose at a concentration of 35% up to some extent

it increases the Bioavailability of the drug to retain the dosage form on the desired site for effective period

of the time.

AICTE Sponsored



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DEVELOPMENT AND IN VITRO CHARACTERIZATION OF SUSTAINED RELEASE

PELLETS OF VENLAFAXINE

Patel Vipul.V., Patel R.P., Patel N.R., Patel S.S.

Shri Jagdishprasad Jabarmal Tibrewala University (Jhunjhunu, Rajasthan),

Email Id.: [email protected]

Abstract:

Sustained release formulation of Venlafaxine hydrochloride based on solution/suspension layering

technology was developed and evaluated. Venlafaxine hydrochloride is a highly water-soluble drug so it

is suitable to develop sustained release dosage form. Venlafaxine is a short acting drug so developed

formulation provides the advantages of sustained release formulations. The developed formulation is

equivalent to US marketed product effexor regarding in vitro release. The developed formulation

provides advantages of easily amenable to mass production using conventional fluid bed processor.

The non-pariel seeds were used as starter seeds. Various binders were tried for binding the drug on to the

non-pariel seeds. After changing the binders and optimizing the parameters, as binder was finalized. A

seal coat was applied on the drug coated nonpareil seeds using the same binder. Finally functional coating

was performed using various concentrations of the polymer. The concentration of polymer was

optimized based on dissolution studies. The dissolution data were matched with the marketed product in

different media. A reproducible batch was prepared using the same formulation and parameters.

Evaluation tests like dissolution, sieve analysis, TD, BD, assay, friability, and angle of repose were

performed.

Stability studies were also performed using walk-in stability chamber for a month at 40 ºC ± 75 % RH.

After six months the pellets were evaluated. Effect of curing on pellets was also determined.

AICTE Sponsored



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MODIFIED RELEASE DRUG DELIVERY SYSTEM FOR ANTIEPILEPTIC DRUG Kundu 2, 3 2, 3 1 2Subrata , Patil Atul , SrinivasanGanga , BorkarNitin

1 2VES College of Pharmacy, Mumbai University ; VerGo Pharma Research Laboratories Pvt.Ltd ;JJT

3 University Email:

Abstract:In the present research work an attempt has been made to design a platform drug delivery technology of single system three layered modified release pellets dosage form. In which loading dose of the drug is present at the surface of the sphere and then delayed release portion and finally the modified release part of the drug at the centre on the core sugar or microcrystalline sphere. The release of drug from the immediate release portion is adjusted through the use of suitable solubilizer and the release from the modified release portion is modulated through the different ratio of rate controlling polymer and the pore former in the multiple dissolution medium. Experimental Design: Formulation Development: One of the model drugs from anti-epileptic category was selected. BCS solubility of the same was carried out to understand their solubility behaviour. Different solublizers were identified to understand the concentration required to achieve sufficient drug release from immediate release portion. Properties of drug were evaluated to understand their process limitation, solubility and dispersibility. Each pellet consists of three portions, Modified release portion at the centre, then delayed release portion and finally solubilized immediate released portion at the surface of the pellet. Drug suspension was prepared with suitable binder and anti-foaming agent in hydro-alcoholic solvent system and first portion of the drug suspension was layered on the core sugar spheres then coated with modified release polymer. Second portion of the drug suspension was layered followed byextended release coat and then coated with delayed release polymer coat. Finally, a solubilizer was dissolved into the third portion of the drug suspension and loaded on the surface of the delayed release portion and considered as immediate release portion. In vitro drug release Studies: Dissolution studies were carried out in 0.1N HCL for 2 hr after that pH 5.5 Phosphate Buffer (0.01M) for 2 hrs followed by pH 6.8 Phosphate Buffer (0.01M) for 14 hrs in USP I dissolution apparatus with 900ml media volume and 50 RPM. (n=6) Result and Discussion: Drug release profile can be sustained up to 18 hour and beyond that also. Drug release profile can be modified with the pore forming agent and rate controlling polymer at modified release layer or delayed release layer. As the pore forming agent concentration increases the drug release increase mostly at the centre of the release profile. Drug release from the immediate release portion can be modified with different solublizers and by changing their concentrations. (RSD < 5%) Conclusion:A modified release polymer based drug delivery system can be designed to provide uniform drug release across the pH conditions for sparingly soluble drugs of antiepileptic categories. This Provides cost effective and novel drug delivery system of existing molecule for life cycle management with higher patient compliance thus provides tremendous business potential by minimising health care management cost. Acknowledgments: VerGo Pharma Research Laboratories Pvt. Ltd. for providing research facility.

Keywords: Delayed release polymer, extended release polymers, Pore former, Solublizer, Platform Drug Delivery Technology.

[email protected]

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SYNTHESIS AND ANTIMICROBIAL STUDIES OF SOME NOVEL SCHIFF BASES

S.Shah N.N*, M.A.Baseer, P.A.Kulkarni. P.G. Department of Chemistry , Yeshwant College, Nanded -431602, Maharashtra ,India

e-mail : ,Abstract:

The chemistry of the caron-nitrogen double bond plays a vital role in progresses of chemistry science.Schiff base exhibit a plethora of bioactivities viz, antitubercular ,anticancer antibacterial, antifungal,analgesic, CNS depressant, anti-inflammatory, anticonvulsant , insecticidal, plant growth inhibitors ,anti mouse hepatitis virus (MHV) , inhibition of herpes simplex virus type 1 (HSV-1) and

adenovirus type 5 (Ad 5 ), anti cancer, anti mosquito larvae and herbicidal activities .Schiff bases are used as protective agent in natural rubber. Schiff's bases includes industrial synthesis of high value life

saving beta lactam antibiotics from class of penicillins and cephalosporins. Schiff bases are used as starting material for the synthesis of various bioactive heterocyclic compounds like 4-thiazolidinones, 2-azetidinones, benzoxazines and formazans. Schiff-base compounds have been used as fine chemicals and medical substrates. These wide application and diverse potential biological activities of Schiff bases prompted us to synthesize new Schiff bases containing heterocyclic moiety and to as certain their microbial activity. A series of Novel Schiff bases were synthesized from 4-[2-(5-Ethyl-pyridin-2-yl)-ethoxy]-phenyl amine by reacting with different aromatic aldehydes via condensation reaction. The

1newly Synthesized Schiff bases were confirmed by TLC, melting points, IR, H-NMR and mass spectra . The compound were evaluated for antibacterial activity against Bacillus subtilis gr +ve, Pseudomonas aeruginosa gr ve , Staphylococcus aureus gr +ve, Escherichia colive and antifungal activity against Aspergillus niger, Aspergillus Flavus, Curvularia, Alternaria. All the compounds shows moderate to good activity against different micro-organisms. Keywords: 4-[2-(5-Ethyl-pyridin-2-yl)-ethoxy]-phenyl amine, aromatic aldehydes, Schiff bases, antimicrobial activity.

[email protected]

AICTE Sponsored



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SIMULTANEOUS ESTIMATION OF METAPROLOL SUCCINATE AND OLMESARTAN MEDOXOMIL FROM CAPSULE DOSAGE FORM BY FIRST ORDER DERIVATIVE

SPECTROSCOPIC METHOD.Swapnil D Jadhav, Shraddha M Kumbhar, Vandana D Patel* and Manish S Bhatia

Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Near Chitranagari, Kolhapur 416013.

Email

Abstract:Metaprolol succinate (MET) and Olmesartan medoxomil (OLM) are used in combination for treatment of hypertension. The present work deals with simple spectrophotometric method development for simultaneous estimation of MET and OLM in capsule formulation (OLSAR-M). The method employed was a first order derivative spectroscopy. For determination of sampling wavelength, 10 ìg/ml of each of MET and OLM were scanned on UV-630 double beam spectrophotometer in 200-400 nm range. The sampling wavelengths were 214 nm for OLM where MET showed zero crossing point and 231 nm for MET where OLM showed zero crossing point in first order derivative spectroscopy. For this method, linearity was observed in 10-90 ìg/ml for MET and 5-45 ìg/ml for OLM. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines. The proposed method can be optimized further for simultaneous estimation of both drugs from biological fluids, used in pharmacokinetic and bioequivalence studies.Keywords: Derivative Spectroscopy, Metaprolol, Olmesartan, OLSAR-M, ICH.

[email protected]

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LIPOSOMES AND ETHOSOMALLY ENTRAPPED CLOTRIMAZOLE: A VIEW TO

IMPROVE THERAPEUTIC RESPONSE OF ANTIFUNGALPatel S.S., Patel V.V., Patel R.P., Patel N.R.

Jagdishprasad Jhabarmal Tibrewala (JJT) University, Rajasthan, India

Abstract:

The aims of the present study were to prepare and evaluate topical ethosomes of Clotrimazole and

comparison of release profile with liposome. Studies were performed on ethosomes containing 20%,

30%, and 40% w/w ethanol. A liposomal formulation, aqueous drug solution and 30% ethanol in water

also prepared for comparative study.

Ethosomes and liposomes were characterized for shape, particle size, and entrapment efficiency. Further,

sonication was done to reduce the size and to get more uniform size and shape and showed better

characterization than unsonicated ethosomes. Comparison of in-vitro skin permeation studies was

carried out using Franz diffusion cells. After the in-vitro release study, cadaver skin was cleaned on both

sides and the drug accumulation in the skin was extracted with 10 ml distilled alcohol and drug

concentration was determined Spectrophotometrically. The stability studies were conducted for a period

of 6 months. Optimized ethosomal formulation (ethosome prepared by sonication and containing 30%

w/w ethanol) showed 162.27ìg/cm²/hr across the human cadaver skin as compared to 28.84ìg/cm2/hr for

liposomes. The size of clotrimazole ethosomes was found to be within the range of 0 18.180 ìm and the

average diameter were found to be 5.595 ìm. Drug accumulated study showed more than 23 % of drug

was deposited into skin and only and 1.26 %, 1.54 % for liposomes respectively. The vesicular size

analysis showed ethosomes prepared without sonication decreased as the concentration of ethanol

increased with the largest vesicles size 5.595 ìm containing 20% ethanol and smallest 3.070 ìm

containing 40% ethanol. Efficient delivery of drug to deep skin strata from ethosomal drug applications

found to be very much beneficial in localizing the drug to desired site, reducing possible side effects and

other drawbacks associated with conventional treatments. Release kinetic of CLZ from ethosomes was

found to be zero order. Ethosomes were found stable in refrigeration and room temperature during

storage of 8 weeks Current work was aimed at exploring ethosome as topical drug delivery vehicle so as

to achieve optimal localized concentration and prolonged action.

AICTE Sponsored



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ANTIBACTERIAL ACTIVITY OF GREEN TEA (CAMELLIA SINENSIS) EXTRACTS

AGAINST VARIOUS BACTERIA ISOLATED FROM ENVIRONMENTAL SOURCES*# # ## ### ###

Sandip Patil , Amit Kumar , P.C.Sharma , Ajay Kumar , Payal Thakur , Vinayak ##

Shinde

# Dept. of Microbiology, Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India -173212.

## Dept. of Pharmaceutics, Tatyasaheb Kore College of Pharmacy, Warnanager. Maharashtra.

###Dept. of Microbiology, Shooloni Institute of Biotechnology and Management Sciences, Solan, Himachal Pradesh. India- 173212.

Abstract:

Tea is cultivated in many countries of the world. India is largest tea (black tea) producer in world

followed by Japan (green tea) and China. In the present study Camellia assamica (Green tea) leaves

extracts were tested for antibacterial activity against various bacteria isolated from environmental

sources. Different bacteria were isolated from sewage samples collected from different places at Solan

Himachal Pradesh. Isolated bacteria were identified by Gram staining and biochemical tests. A total of

six bacteria were identified at Department of Microbiology at SILB Solan (H.P) Green tea leaves

extracts were tested for antibacterial activity. Tea leaves were collected from Palampur, Himachal

Pradesh. Three different extracts were prepared by using standardized protocols. All the extracts were

tested for antibacterial activity by disc diffusion method. Antibacterial assay was performed at 10µl,

20µl, and 30µl concentrations. Significant antibacterial activity was reported for all extracts with results.

Aqueous extracts has shown little antibacterial activity against six bacteria isolated. Maximum

antibacterial activity was found in methanolic extracts. Our study reflects the chemotherapeutic use of

green tea.

Key Word Green Tea, Extract, Antimicrobial Activity.

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SYNTHESIS OF SILVER NANOPARTICLES USING ONE-POT GREEN METHODKulkarni A.S., Karande K.M., Mastud P.R.

Satara College of Pharmacy, Satara. Plot No.1539, Behind Spicer India Ltd., New additional M.I.D.C., At Degaon, Satara. 415 004 (M.S.)

Abstract:

The current technique used for the preparation of silver nanoparticle is a modification of one pot

method described by Vigneshwaran et al. Nanoparticles can be synthesized either chemically or

biologically. The chemical process for synthesis of silver nanoparticles is more elaborate and leaves

behind toxic effect that adversely affects the ecosystem on the other hand green synthesis of Ag Nps is

less time consuming, less costly and more ecofriendly. Stable silver nanoparticles have been

synthesized by using soluble starch as both the reducing and stabilizing agents; this reaction was carried 0

out at 15 psi, 121 c for 5 min. Nanoparticles thus prepared are found to be stable in aqueous solution over

a period of one week at room temperature. Silver nanoparticles are prepared using soluble starch acting

as both the reducing and stabilizing agents. The aldehyde terminal of soluble starch is used to reduce

silver nitrate while the starch itself stabilized the silver nanoparticles. The nanoparticle was further

characterized by UV VIS spectroscopy which revealed that the formation of Ag NPs by yielding the

typical silver Plasmon absorption maxima at 430 nm. FTIR spectra revealed the involvement soluble

starch for reduction of silver nitrate.

Keywords: Silver nanoparticles; soluble starch; Green chemistry; Sonication; Bioreduction.

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COMPARATIVE ANTI-INSECT ACTIVITY OF ACETONE EXTRACT OF DIFFERENT

SEEDSRathod A.M.* Salunkhe R.M., Nadaf S.J. Jarag R. J., Killedar S.G.

Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra - 416013, [email protected]

Abstract:

Traditional medicines are still very commonly used in India for anti insect purposes. The

indiscriminate use of chemical pesticides has given rise to many serious problems, including toxic

residue, increasing cost of application, environment pollution, development of resistance and hazards

from handling has been reported from all over the world. Therefore, the development of bio-insecticides

has been focused as a viable pest control strategy in recent years for ecological, safety and economical

alternative to synthetic one in managing pests. Hence the demand for search of natural plant products has

been increased. Present study was aimed to screen and compare the anti-insect activity of acetone

extracts of five different seeds. The insect repellant activity was carried out using Tribolium castaneum

as test organism by ring arena method. Insecticidal activity was performed by contact poison test

method. The activity was performed by using 30 fresh first generation insects each time and %

repellency and insecticidal activity was determined along with control (plain acetone) and standards

(Citronella oil 10% in acetone for repellant test and Celphos 5% w/v in acetone for insecticidal activity)

at tested concentrations. Study reveals that neem seed extract shows very promising insect repellant and

insecticidal activity (78% and 95%) respectively, followed by custard apple (62% and 85%),

sapota,(60% and 81%) fenugreek (55% and 72%) and tamarind seeds (45% and 58%).

Keywords: Anti-insect activity, Tribolium castaneum.

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PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF FLOWERS

(INFLORESCENCE) OF SACCHARUM OFFICINARUM LINN.*Bhore N. V., Pishawikar S. A., Mahamuni S. S., Kadane S. A., Chavan S. V.

Bharati Vidyapeeth College of Pharmacy, Department of Pharmaceutical Chemistry,Near Chitranagari, Kolhapur (M.S.) 416013, India.

Presenting author's E-mail: Abstract:

Saccharum officinarum Linn having flowering region known as arrowing has soft-rinded, puffy

nature. The extracts of Sugarcane inflorescence was carried out using petroleum ether, chloroform,

Dichloromethane, Ethyl acetate, n-Butanol, Methanol and Distilled water as solvents. The

phytochemical investigation of extracts revealed the presence of alkaloids, tannins, anthraquinones,

reducing sugars, saponins, flavonoids, polyphenols, steroids and terpenoids. Some of these fractions

were screened for free radical scavenging activity (Antioxidant) using 2, 2-diphenyl-1-picrylhydrazyl

radical (DPPH). In DPPH scavenging assay, free radicals are involved in the process of lipid

peroxidation and play a cardinal role in numerous chronic diseases like cancer, coronary heart disease

and ageing. Thus the ability to scavenge free radicals in order to minimize oxidative damage to living

cells is very important. The better scavenging activity of Saccharum officinarum L. could be linked to

the presence of secondary plant products like flavonoids and phenols, which have the ability to

scavenge hydroxyl radicals and lipid peroxy radicals. On the basis of activity shown further

investigation as separation, isolation and characterization of chemical constituents using HPTLC and

spectroscopic method will be done.

Keywords: Saccharum officinarum L., Inflorescence, Antioxidant activity, DPPH, Secondary

metabolites.cal drug delivery vehicle so as to achieve optimal localized concentration and prolonged

action.

[email protected]

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APIS MELLIFICA (HONEY) ENHANCES WOUND HEALING ACTIVITY OF TRIDAX PROCUMBENS, CURCUMA LONGA AND TERMINALIA CHEBULA

Salunkhe R.M.*; Rathod A. M., Jarag R. J., Pawar A.A., Ninganure S.S.

Department of Pharmacology, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur,

Maharashtra, India-416013 [email protected]:

Aim of our work is to prepare cream containing combination of juice of Tridax procumbens, aqueous

extract of Curcuma longa, dried ripe fruits of Terminalia chebula and Apis mellifica (honey). Cream was

evaluated for physical parameters and wound healing property by excision and dead space wound model.

The plants used were authenticated in botany department in Shivaji University, Kolhapur. Protocol

approved by IAEC BVCOP Kolhapur. The cream was prepared by using extract of fresh leaves of T.

procumbens, decoction of C. longa, decoction of fruit of T. chebula and A. mellifica. Four different

creams were formulated by different percentage of excipients. All formulations F-1, F-2, F-3 and F-4

were subjected for preliminary evaluation like PH, viscosity, spreadability and extrudability. The

prepared cream showed considerable difference response in both models as comparable to those of a

standard drug Nitrofurazone ointment (0.2% w/w NFZ) in terms of percentage closure, time of

epithelization, scar size. F-4 showed batter physical parameters for cream so it can use for wound healing

studies. The time required for complete epithelization in excision type of wound healing was 10.4 days in

test group compare to 17.4 days in control group and 15.8 days in base treated group. Scar size studies

indicated that the list scar size was found for formulation F-4 applied wound when compared with control

and base formulated treated groups. Histopathological studies for dead space wound model showed more

collagen content, fibroblast population, infiltrating cells and thickness of the tissue which confirm

enhancement wound healing activity of T. procumbens, C. longa, T. chebula with A. mellifica.

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COLORIMETRIC METHOD DEVELOPMENT FOR REPAGLINIDE USING FLOWER

EXTRACT OF DELONIX REGIA*Chavan D. S., Pishawikar S. A., Nale A. B., Garud S. H. , Khetmar S. S.Bharati Vidyapeeth College of Pharmacy, Department of Quality Assurance,

Near Chitranagari, Kolhapur (M.S.) 416013, India. Presenting author's E-mail:

Abstract:

There are natural plant pigments available in shoots, flowers, fruits or roots or plants that can be extracted

and used as acid base indicators. The use of natural dyes as acid-base indicators was first reported in 1664

by Sir Robert Boyle in his collection of essays “Experimental History of Colors”. It is found that natural

pigments obtained from plants have been used as pH indicators in titrometric analysis. Colorimetric

method using flower extract of Delonix regia was developed for estimation of Repaglinide based on

following principal - Anthocyanins are polyphenolic in nature. In tautomeric form of phenol in general is

going to be acidic, as electrons on oxygen are going to get delocalized in aromatic ring, making it easier +

for removal of H as H i.e. proton making it acidic. While amide due to presence of loan pair of electron

on nitrogen is going to show basic nature which contributes in formation of color-complex between

amide moiety present in drug and the color pigment obtained from Delonix regia Beer's law is followed

in the ranges from 5-40µg, Correlation Coefficient was found to be 0.9996. Result of accuracy and

precision shows that developed method is simple, precise and accurate.

[email protected]

AICTE Sponsored



P-36

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PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND ANTIULCER ACTIVITIES OF PALMYRA PALM

A.V.Yadav, Madhuri Patil, Tejaswini Kakade*,Sana mokashi.

GIPER, Limb Satara Department of Pharmacognosy and Phytochemistry, Survey No. 990, Tal. &

Dis. Satara, pin code-4151002 Maharashtra India.

E-Mail:[email protected]

Abstract:

The fruit of Palmyra palm. (Borassus flabellifer Linn) belonging to family Areaceae reported to be useful

in treatment of gastric ulcer. In pharmacological screening the effect of different extracts (300mg/kg) p.

o. of fruits of Palmyra palm. was evaluated for their antiulcer profile by using aspirin Pylorus ligation and

ethanol induced models using albino rats. Various parameters like volume of gastric content, pH of

gastric content, free acidity, total acidity, ulcer index were determined. These results were comparable to

that of standard drug (Ranitidine). Treatment with aqueous extract of fruits of Palmyra palm

significantly showed the antiulcer activity as compared to control and other extracts. The

histopathological study of stomach also supported the above results. The phytochemical analysis carried

out revealed the presence of saponins, tannins, flavonoids, carbohydrates, amino acids and phenolic

compounds in the extracts.

Keywords: Anti-ulcer, Palmyra palm. Aspirin pylorus ligation, Ranitidine.

AICTE Sponsored



P-37

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FORCED DEGRADATION STUDY OF STRONTIUM RANELATE ACCORDING TO ICH

GUIDELINESAshwini S. Swami*, Sachin A. Pishawikar and Harinath N. More

Department of Pharmaceutical Chemistry, Bharti Vidyapeeth College of PharmacyKolhapur, Maharashtra 416 013. (INDIA) Email:[email protected]

Abstract:

Strontium ranelate (SR) is chemically designated as distrontium 5-[bis (2-oxido-2- xoethyl) amino]-

4-cyano-3-(2-oxido-2-oxoethyl) thiophene-2-carboxylate is used for treating osteoporosis as well as

postmenopausal osteoporosis. SR has a dual mode of action, both increasing bone formation and

decreasing bone resorption, which rebalances bone turnover in favour of bone formation and increases

bone strength. Forced degradation studies help facilitate pharmaceutical development as well in areas

such as formulation development, manufacturing, and packaging, in which knowledge of chemical

behaviour can be used to improve a drug product. The International Conference on Harmonization (ICH)

guidelines indicates that stress testing is designed to determine the intrinsic stability of the molecule by

establishing degradation pathway in order to identify the likely degradation products and to validate the

stability indicating power of the analytical procedure used. ICH guidelines 'stability testing of new drug

substances and products' Q1A(R2) and (Q1B) requires that stress testing should be carried out to

elucidate the substance. The present study involved the effect of acid, alkali, light, hydrogen peroxide

and temperature on the stability of SR. HPLC method has been developed to study the SR in pure form as

well as for degradation products. In the present work, degradation study using HPLC according to ICH

guides for strontium ranelate show that thermal treatment, UV light (up to 24 hrs) and alkali treatment

have negligible effect on the degradation of SR, whereas acidic and oxidative environment degrade the

API to significant extent.

Key words: osteoporosis, strontium ranelate, HPLC, forced degradation, ICH guidelines

AICTE Sponsored



P-38

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PHYSICOCHEMICAL EVALUATION OF POORLY WATER SOLUBLE DRUG BY SOLID

DISPERSION TECHNIQUEIndapure Deepak D.*,Salunkhe Sharad B.,Shinde Sunita S.Shete Amol S.

Shri Vitthal Education & Research Institutes College of Pharmacy, Pandharpur. Tal: Pandharpur. Dist.: Solapur, Maharashtra, India, E-mail*: [email protected]

Abstract:

For an orally administered drug to get into systemic circulation, it must be sufficiently soluble to dissolve

in the gastro-intestinal fluid. Once the drug has dissolved it must then be sufficiently permeable to be

absorbed across the gastrointestinal wall. Poorly soluble drugs may benefit from formulation

approaches that overcome poor solubility and dissolution rate limited bioavailability. The enhancement

of oral bioavailability of poorly water soluble drugs remains one of the most challenging aspects of drug

development. Complexation, precipitation, adsorption, salt formation, particle size reduction, etc. have

commonly been used in industry to solubility of the drug, there are practical limitation with these

techniques the desired bioavailability enhancement may not always be achieved. Therefore formulation

approaches are being explored to enhance bioavailability of poorly water-soluble drugs. One such

formulation approach that has been shown to significantly enhance absorption of such drugs is to

formulate prepare solid dispersion using spray drying technique and solvent evaporation method.

Atorvastatin is a hypolipidemic drug, which is often administered orally. Atorvastatin exhibits very

slightly soluble and as a consequence it exhibits low bioavailibity after oral administration. Therefore

the improvement of Atorvastatin dissolution from its oral solid dosage forms is an important issue for

enhancing its therapeutic efficiency. The present study was enhancement of dissolution rate of poorly

water soluble drug. The solid dispersion was using porous carrier where Aerosil200 was selected as

adsorbent. By spray drying and solvent evaporation method the resultant complexes were evaluated for

drug content, dissolution rate, XRD, FTIR, DSC and SEM.

Keywords: Atorvastatin, Spray drying, solvent evaporation, solubility, etc.

AICTE Sponsored



P-39

EXTRACTION OF HIBISCUS ROSA SINENSIS LINN. AND IT'S USE IN ANALYTICAL

METHOD DEVELOPMENT

Jadhav D. V. * , Pishawikar S. A., Kalantre U. L. , Khabale R. S. , Raut J. N.

Bharati Vidyapeeth College of Pharmacy, Department of Pharmaceutical Chemistry,

Near Chitranagari, Kolhapur (M.S.) 416013, India.

Presenting author's E-mail: [email protected]

Abstract:

Purpose: There are different types of pigments obtained from plants such as Chlorophylls, Carotenoids,

Flavonoids, Phytochroms, Phycobilins, Betalains. These are used for coloring cloths, as food colorants

and in analysis as acid- base indicators. A successful attempt has been done for the first time to use them

in developing analytical spectrophotometric method for amide linkage containing drug. Methods: A

simple, accurate, sensitive and reliable method have been developed for the determination of

glybanclamide in bulk and pharmaceutical formulation. Using extract of Hibiscus Rosa Sinensis Linn. A

calibration curve was constructed at optimum experimental conditions using absorbance values at 605

nm versus concentration in the range of 5 to 40 µg/ml. High value of the correlation coefficient

(r=0.9996) indicates a good linearity and adherence of the method to Beer's law. Developed

method is validated using ICH QB2 guidelines Result: The absorbance of the pale yellow colored

complex was measured spectrophotometrically at 605 nm against reagent blank. From Calibration

graphs Beer's law is followed in range of 5 to 40µg/ml with correlation coefficient 0.9996, while the

LOD and LOQ was 0.27, 0.79 respectively. Conclusion: As use of extracted pigment is done for amide

group containing class of hypoglycemic agents, further study to develop simple, accurate, sensitive and

reliable Visible Spectrophotometric methods for different types of drug formulations can be done and

the methods can be used in carrying out routine analysis.

Keywords: Hibiscus Rosa Sinensis Linn, calibration curve, Beer's law, ICH QB2 guidelines.

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Date post:	31-Mar-2016
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