Presented by:Acotsca pratama* ( Technische universitaet Clausthal germany)

Maulidya Rosaline2 ( State University of Surabaya Indonesia )

Alfi Lailiyah3 ( University of Indonesia )

Traviata Prakarti4 ( University of Indonesia )

Nur Herda Wati Nisa5 ( University of Indonesia )

Eky Lukman Hakim6 ( Universitas Gadjah Mada of Indonesia )

Oral Presentation“Spirulina as Potential Biological Agents Protein

Needs Best Solution for Pregnant Woman”

Background

Point of problems

Main Solutions

Method

Conclusion

These factors closely related to the nutritional aspect of the sociaty, especially children’s health and

pregnant women (Harahap, 2007).

Three of main factors quality index of life :

Education

Health

Economy

There are four nutrition problems in Indonesia those are :

2. Iodium deficiency problems

1. Malnutrition

4. Vitamin A deficiency

3. Iron deficiency anemia

OUR Fact in Indonesia

The average of maternal mortality rate (MMR) was recorded at 359 per 100 thousand live births in 2008-

2012 (Indonesian Demographic

Health Survey/ IDHS, 2012)

This death rate is much

INCREASED

Compared the result of IDHS 2007 which

reaching 228 per 100 thousand live

births

Infant Mortality Rate (IMR) is 34 per 1.000 live births

(IDHS, 2007)

Maternal Mortality trends in Selected ASEAN Countries

Source: http://www.unicef.org/indonesia

Trend of Maternal Mortality Rate in Indonesia (IDHS, 2012)

Source: Ministry of Health in Indonesia, 2012

MDGS Target for MMR is

102/100.000 live births

Trend of Infant Mortality Rate

Source: http://planipolis.iiep.unesco.org/upload/Indonesia/Indonesia_MDG_2010.pdf

Frequency Distribution IMR in Indonesia (IDHS, 2007)

Most of the contributors to infant

mortality rate in Indonesia is in the

eastern part of Indonesia which includes Maluku, Papua and Nusa

Tenggara

What happening?

One of the most important causes of maternal mortality is Chronic Energy Deficiency (CED) which can cause

bleeding and infection

According to UNICEF (2012), one of factors causing CED experienced by

pregnant women is the lack of nutritional intake of food,

especially protein intake

Research in Nusa Tenggara Barat

The results of this study showed that there is a significant relation between CED and incidence of LBW. The same study ever conducted by Hidayati in Mataram, Nusa Tenggara Barat. The subjects were exposed to the CED has

a probability of 4.71 times more likely to give birth to LBW (Theses in 2005)

(continue)

According the result from Budijanto study (2000), showed that when pregnant woman has lower of nutritional status with ≤9 kg weight gain and upper arm circumference less than 22 cm will have the risk of having a baby with low birth weight (less than 2.5 kg)

Pregnant women need protein intake around 55-60 grams per day

100 grams of soy milk contains 23 grams of protein

100 grams of tempeh contains 41 grams of protein

100 grams of cow's milk contains 3.2 grams of protein

100 grams of beef contains 21 grams of protein

NOT ENOUGH

What is Spirulina sp.?

Description• Unicelluler microalgae and

classified as microscopic filamentous cyanobacterium belonged

• Fairly HIGH protein content• Protein content per 100 grams

of spirulina sp. is 57,5 = amount of protein required by pregnant woman

• Bluish green microalgae whose life widespread in all ecosystems

Types of Food Protein Content

Spirulina 60-70%Meat and fish 15-25%Chicken 24%Soybean 35%Milk powder 35%Legumes 25%Egg 12%Cereals 14-18%

[Comparation of Spirulina protein among others ( Yosta, E, et al. 2010. Algae Oil Extraction from Spirulina )]

[Ordinary Pregnancy Milks ]

METHOD

Data Collection• The data obtained in this

study is primary data. The primary data obtained by giving questionnaire to the respondent.

• The collection of data through questionnaires spend for a month.

Data Analysis• This research using

quantitative descriptive method.

• There are three variables such as variable knowledge, attitudes, and action.

• Population students from public health faculty University of Indonesia and students from State University of Surabaya majoring in biology.

• Technique sampling Quota sampling.

RESULTThe data obtained from this study provide the results of level of knowledge about the potential health Spirulina sp. For pregnant women as well as illustration of action and attitude from respondent toward this product, and will be presented in the following graph:

Fig. 1 Overview of respondents knowledge level toward Spirulina sp.for maternal

Fig. 2 Overview of Respondents Attitudes toward Dairy Spirulina sp.

Fig. 3 Overview of the Respondents actions toward Dairy Spirulina sp.

Table 1.Correlation of Knowledge with Attitude

Attitude

TotalNegative attitude

Positive attitude

Knowledge Poor Knowledge

Count 2 0 2

% within Knowledge 100,0% 0,0% 100,0%

Good Knowledge

Count 4 24 28

% within Knowledge 14,3% 85,7% 100,0%

Total

Count 6 24 30

% within Knowledge 20,0% 80,0% 100,0%

Table 2.Correlation of Knowledge with Action

Action

TotalNegative action

Positive action

KnowledgePoor Knowledge

Count 1 1 2% within

Knowledge 50,0% 50,0% 100,0%

Good Knowledge

Count 1 26 27% within

Knowledge 3,7% 96,3% 100,0%

Total

Count 2 27 29% within

Knowledge 6,9% 93,1% 100,0%

CONCLUSION

Respondents with a better level of knowledge about the potential of Spirulina sp. for maternal health have a positive attitude and actions towards dairy products of Spirulina sp. Spirulina sp. can be one of the potential solution to overcome the problems of maternal mortality rate (MMR) in Indonesia, particularly in eastern Indonesia.

There is a relation among knowledge with attitudes and respondents action toward dairy products Spirulina sp.

Comparation Spirulina milk between the ordinary Pregancy Milks

References• Yudiati, Ervia, et al. 2011. Antioxidant Activity and Toxicity Methanol Extracts and Crude Pigments

Spirulina sp. Vol. 16 (4) 187-192. Semarang: Diponegoro University Belcher, Hilary and Elica Sware. 1978. A Beginner’s Guide to Freshwater of Algae. London: Institute of Terestrial Ecology: Natural Environment Research Council

• Belcher, Hilary and Elica sware. 1978 A Beginner's Guide to Freshwater Algae of. London: Institute of Terrestrial Ecology: Natural Environment Research Council

• Budijanto, et al. 2000 The risk of Low Birth Weight Babies (LBW) in Madison County Health Center Balorejo. Medika .September No. XXVI 9 Year 2000; p 566-569Yosta, E, et al. , 2010. Algae Oil Extraction from Spirulina Sp. New Raw Material Alternative Discourse on Biodiesel Production Process. Surabaya: Surabaya Institute of Technology

• Ward, the Supreme Faithful. 2012 Milk Processing Technology. Surakarta: Slamet Riyadi University

• Yosta, E, et al. , 2010 Algae Oil Extraction from Spirulina Sp. New Raw Material Alternative Discourse on Biodiesel Production Process. Surabaya: Surabaya Institute of Technology

• Zaenab, R., Joeharno. , 2008 Several Risk Factors The incidence of LBW in the Al-Fatah hospital Ambon period of January to December of 2006 Available from: http: / //weight-body-low-birth-bblr.html

• UNICEF, Indonesia 2012: annual report 2012 Unite for Children• Simanjuntak, David and Etti Sudaryati. 2011. Nutrition In Pregnancy and Breastfeeding. North

Sumatra: Faculty of Public Health, University of North Sumatra• Belliger Edward G. and David C. Sigee. 2010 Freshwater Algae: Identification and Use as

Bioindicators. John Wiley & Sons, Ltd.

“ Thing about the generations and to say we want to make a better world for our children and our children’s children its better world for them and thing if they can make it a better place”.

Increasing health side for mom and children while make our quality of life much more better .

Thank you and Last We Say

The analysis of proteins have 2 methods, that qualitative and quantitative. Qualitative

analysis has

Xantoprotein reaction, Hopkins-Cole reaction, Millon reaction, Nitroprusida reaction,

and Sakaguchi reaction. While the Quantitative analysis has Kjeldahl method, titration

formol, Lowry method, visible spektrofotometry

• Lowry method is the development of Biuret method. This method has 2 reactions. First, Complex of Cu(II)-protein will be made like biuret method, which in alkalis Cu(II) reduced to Cu(I). Cu+ reducing Folin-Ciocalteu reagent, complex of phosphotungstat produce heteropoly-molybdenum blue because oxidation reaction the aromatic by catalysis of Cu which give Intensif blue colour in order to detected with kolorimetri. The power of blue colour based on residues of tryptophan and tyrosine. The advantage this method is more sensitive ( 100 times) than Biuret method so needs few sample of protein. The detection limits approximately at concentration 0.01 mg/mL but this method has much interference because the sensitivity (Lowry et.al 1951).

More advantages • We have more benefits acid such as: linoleic

acid (LA), docosahexaenoic acid (DHA) and arachidonic acid (AA) are in spirulina, so do some vitamin such as vitamins B1 (thiamine), B2 (riboflavin), B3 (nicotinamide), B6 (pyridoxine), vitamin C, vitamin A and vitamin E. It also a source of potassium, calcium, magnesium, sodium and zinc. Protein has an important source of calories for pregnant woman because in the same time they need more 10 grams of protein than usual. Therefore, our products also provide protein content of 55-70% which useful for pregnant women.