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St.CroixEstuaryProjectInc.March,2006
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Objective:
The purpose of this kit is to provide a set of xed sampling sites and sampling protocols that can be used over the long term to quickly and
ef ciently assess the health of the St. Croix River Estuary. Detailed eld studies should be conducted at 3 to 5 year intervals or when annual
assessments show there are problems or changes that need more detailed study.
Protocols:
There are three components to the eld survey:
. Chemistry and Bacteriology - Water and sediment samples will be taken for ammonia, nitrite, nitrate, phosphate, salinity, conductivity,
temperature, pH, and redox, dissolved oxygen, total coliforms, and E. coli.
2. Intertidal Biota - The presence or absence of indicator species from the intertidal area will be surveyed.
. Subtidal Biota and Sediments - The presence or absence of indicator species and bottom condition will be determined by remote video ordiver generated video.
Field teams of not less than 2 trained staff and/or volunteers will be responsible for each component.
iming:
The preferred time for sampling is August and September, but no later than October 31.
Equipment:
a) Subtidal Sampling - camcorder, underwater camera, AC source, computer with movie application. Diver, boat, and Underwater camera or
remote underwate camera used from a kayak or canoe.
b) Intertidal Biota - Boots, Clipboard and data sheets.
c) Sediments & Water -ORP/pH/temperature meter; test Strips, bottles and kits for: sulphides, salnity, dissolved oxygen, phosphates, ammo-
nia, nitrite, nitrate, bacteria; gloves and data sheets.
. Study Zones
4. Study Sites
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Field Data Sheet
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ZONE JONE J - Buchanan Hill to Calais Golf
Club - Station Locations
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ONE J - Station 1
ocation: This station is located at The Cove above the Inter-
national Bridge in St. Stephen.
iming: This station should be visited between 2 hours to low
water and 2 hours after low water.
ccess: Park in the Parking area just south of the strip park.
his is probably public parking, but permission should be
sought from the residents of the adjacent house. Alternate Park-
ng exists in the Park at the top of Bucanan Hill. Access to the
shore is at the Kiosk overlooking the Cove.
ampling Requirements
a) Biota
b) Sedimentsc) Water
ite Data
2003 2004
Total Coliform >20000 >20000
E.coli 800 85
005 2006 2007
Total Coliform 20000 5660
E.coli 85 283
Nitrate 5
Nitrite 0
Phosphates 5 15
Ammonia 0.25
Temperature 6.1 16.34
pH .95 6.27
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003 004
Total Coliform 42 000
E.coli 42 000
Nitrate .5
Nitrite
Phosphates 5
Ammonia .4
Temperature 4.1
pH .09
Redox 31.1
2005 006 007
Total Coliform 040
E.coli 2
NitrateNitrite
Phosphates
Ammonia .25
Temperature 6.84
pH .98
ZONE J - Station 2
Location: his station is located adjacent to Pizza Delight and the
public wharf.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the public parking area across the stree behind the
library. Access to the shore is gained beside the wharf.
Sampling Requirements
a) Biota
b) Sediments
c) Water
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2003 2004
Total Coliform > 20 000 > 20 000
E.coli > 20 000 6 520
2005 2006 2007
Total Coliform >20 000 560
E.coli >20 000 8
Nitrate 0
Nitrite 0
Phosphates 30Ammonia .25
Temperature 23.2 7.1
pH 5.1 .3
ZONE J - Station 2A
Location: his station is located behind the Clarke Building on
Milltown Blvd.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the Parking Lot behind the Clarke Building.
Sampling Requirements
a) Biota
b) Sediments
c) Water
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Site Data
002 2003 2004
Total Coliform 200 000 31 000
E.coli 6 500 9 000
005 2006 2007
Total Coliform TNTC
E.coli TNTC
Nitrate 1
Nitrite 0
Phosphates 15
Ammonia 0
Temperature 18.44
pH 6.83
ZONE J - Station 2B
Location: his station is located in front of the green picnic kiosk
located on Budd Ave.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the public parking area across the stree behind the
library, on Budd Avenue. Access to the shore is gained at the kiosk.
Sampling Requirements
a) Biota
b) Sediments
c) Water
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2003 2004
otal Coliform 42 000
.coli 10
Nitrate 0
Nitrite 0
hosphates 0
mmonia 0.2
emperature 10
pH 7.07
edox -125.1
2005 2006 2007
otal Coliform 5920
.coli 296
Nitrate 0Nitrite 0
hosphates 5
mmonia 0.25
emperature 19.36
pH 5.96
ZONE J - Station 3
Location: his station is located at the southwest corner of the
seniors complex parking lot
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the seniors complex parking area
Sampling Requirements
a) Biota
b) Sediments
c) Water
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ZONE J - Station 4
Location: his station is located where the Dennis Stream ows into
the St. Croix.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area outside of the warehouse, adjacent to the
bridge, please do not block doors.
Sampling Requirements
a) Biota
b) Sediments
c) Water
2002 2003 004Total Coliform 10 820
E.coli 10 100
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pH
Redox
2005 2006 007
Total Coliform 80
E.coli 49
Nitrate 0
Nitrite 0
Phosphates
Ammonia 0Temperature 20.72
pH .85
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ONE I
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ZONE I - Station 5
Location: his station is located at The Narrows. Take the Donald
Road off the Ledge Road.Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.
Sampling Requirements
a) Biota
b) Sediments
c) Water
ite Data
2003 2004
Total Coliform 260
E.coli 10
Nitrate 0
Nitrite 0.1
Phosphates 5Ammonia 0.25
Temperature
pH
Redox
005 2006 2007
Total Coliform 2000 1820
E.coli 7 91
Nitrate 0
Nitrite 0
Phosphates 0 5
Ammonia 0
Temperature 7.9 19.60
pH .4 7.3
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ZONE H
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ZONE H - Station 6
Location: his station is located at the Ledge Loop.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.
Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data
003 2004
Total Coliform
E.coli
Nitrate
Nitrite .15
Phosphates
Ammonia .5
Temperature .6
pH .53
Redox 54.1
2005 006 2007
Total Coliform >2000 780
E.coli 782 39
Nitrate 0
Nitrite 0
Phosphates 0 5
Ammonia
Temperature 7.8 7.06
pH .2 .13
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ONE G
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ite Data2003 2004
Total Coliform 840
E.coli 110
Nitrate 0.5
Nitrite 0.07
Phosphates 5
Ammonia 0.12
Temperature 10.4
pH 6.72
Redox 6.42
2005 2006 2007
Total Coliform 660
E.coli 33
Nitrate 0
Nitrite 0
Phosphates 5Ammonia 0
Temperature 18.62
pH 7.05
ZONE G - Station 7
Location: his station is located across the highway from Minute
Muf er.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the parking lot at Minute Muf er.Sampling Requirements
a) Biota
b) Sediments
c) Water
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ZONE G - Station 8
Location: his station is located at the beach by the Oak Bay Camp-
ground.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data
003 2004
Total Coliform 00
E.coli 10
Nitrate
Nitrite .7
Phosphates
Ammonia .15
Temperature .5pH .83
Redox 16.8
2005 006 2007
Total Coliform 9 440
E.coli 20
Nitrate 0
Nitrite 0
Phosphates 0 5
Ammonia
Temperature 22 8.49
pH 7.8 .77
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ZONE F
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ZONE F - Station 9
Location: his station is located at the bridge over the Waweig River
at Gilmans Corner.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.
Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data
003 2004
Total Coliform
E.coli
Nitrate
Nitrite
Phosphates
Ammonia
Temperature
pHRedox
2005 006 2007
Total Coliform 00
E.coli 8
Nitrate
Nitrite
Phosphates
Ammonia .25
Temperature 6.46
pH .53
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ZONE E
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ZONE E - Station 10
Location: his station is located at the Ganong Nature Park at Todds
Point.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.Sampling Requirements
a) Biota
b) Sediments
c) Water
ite Data2003 2004
Total Coliform 60
E.coli
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ZONE D
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ZONE D - Station 12
Location: his station is located at the Island View Campground at
Sandy Point.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.Sampling Requirements
a) Biota
b) Sediments
c) Water
ite Data
2003 004
otal Coliform
.coli
Nitrate .5
Nitrite .12
hosphates 2
Ammonia .12
emperature 0.8
pH .06
edox 244.1
2005 2006 007
otal Coliform 271 4920
.coli 22 246
Nitrate 0 2
Nitrite 0
hosphates 15
Ammonia
emperature 15 7.70
pH .1 .69
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ONE C
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ZONE C - Station 13
Location: his station is located at the Garcelons in Johnson Cove.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area.
Sampling Requirementsa) Biota
b) Sediments
c) Water
ite Data
2003 2004
Total Coliform 50
E.coli >10
2005 2006 2007
Total Coliform 04
E.coli
Nitrate
Nitrite
Phosphates 0
Ammonia
Temperature 6.6
pH .7
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ONE B
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ZONE B - Station 14
Location: his station is located at the furthest end of the main build-
ing on your right at the St. Andrews Biological Station on Brandy
Cove Rd.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: Park in the area designated for parking
Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data003 2004
Total Coliform 20 504
E.coli 10 11
Nitrate .5
Nitrite .07
Phosphates
Ammonia .2
Temperature 2.6
pH .78
Redox 9.1
2005 006 2007
Total Coliform 090
E.coli
Nitrate
Nitrite
Phosphates 0Ammonia
Temperature 5.9
pH .9
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ONE A
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ZONE A - Station 16
Location: Heading into St Andrews from St Stephen, this station is
located by turning right at the Block house onto Joes Point Road.
Continue on Joes Point Road unitl you cross a little bridge. The
bridge is at this location.
Timing: This location should be visited between 2 hours to low waterand 2 hours after low water.
Access: Be careful where you park. There is no real safe place to
park, so pull over to the side of the road and leave hazard lights on.
Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data003 2004
Total Coliform 070 1400
E.coli 0 300
Nitrate .5
Nitrite
Phosphates 0
Ammonia .12
Temperature 1.8
pH .75
Redox 23.3
2005 006 2007
Total Coliform 820
E.coli 91
Nitrate
Nitrite
Phosphates 5Ammonia
Temperature 5.42
pH .11
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ZONE A - Station 15
Location: his station is located at the furthest point east in St An-
drews. Enter on the main road of St Andrews and drive until you are
adjacent to the Kiwanis Campground.
Timing: This location should be visited between 2 hours to low water
and 2 hours after low water.
Access: There is available parking on the side of the road.
Sampling Requirements
a) Biota
b) Sediments
c) Water
Site Data
003 2004
Total Coliform 504
E.coli 11
2005 006 2007
Total Coliform >2000 040E.coli 3 0
Nitrate
Nitrite
Phosphates 0 0
Ammonia
Temperature 26 6.12
pH .5 .41