Date post: | 18-Dec-2014 |
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Computational Modelling of Epithelial to Mesenchymal Transition
Tariq Abdulla1, Ryan Imms1, Jean-Louis Dillenseger2,3,
Jean-Marc Schleich2,3, Ron Summers1
1.Dept Electronic & Electrical Engineering, Loughborough University, UK
2. LTSI, University of Rennes 1, France
3. INSERM, U642, Rennes, F-35000, France
http://www-staff.lboro.ac.uk/~lsrs1
Outline
• Introduction to the team
• Heart Development
• Multiscale Modelling
• EMT Simulations
International Team
Heart Development
How … from …To …
Primitive heart tube Heart at the end of development
Epithelial to Mesenchymal Transition
Notch Delta
Muscular Septum
Membranous Septum
Nature
Multiscale Modelling
Modelling Scales
Hunter P, Robbins P, Noble D (2002) The IUPS human physiome project.Eur J Physiol 445 1-9
EMT Simulations
In vitro EMT
Wildtype Notch1 BMP2
L. Luna-zurita et al. “Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation,” The Journal of Clinical Investigation, vol. 120, 2010.
CPM Model
22
',))'(()),(())'(()),(( )()()1( VvSsJE vs
xxxxxx
Compucell3D
Mitosis Simulations
Mitosis Simulations
How does an increase in mitosis prevent EMT from happening?
2D Separation
No Mitosis No MitosisMitosis Mitosis
3D Invasion
Thank you for your attention
Any questions?
Validation Step
Our simulations are not validated but we intend to do this.
To illustrate how this can be done, here is an existing example of a
validated simulation.
Somitogenesis